Rroxscaffold_4G00328710

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0015798
Physical Location & Seq
Reverse (-)
61789760 .. 61796471
6712 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00328710.1

Sequence Viewer

Length: 2565 bp
ATGAGGGAGAGGATGGAGGACTACTTGAGAAAAAACTTCGACGTCGAACCAAAACGACCTTCCGAGGAGGCCTTGAGGCGCTGGAGACAGGCCGTCTCCGTCGTCAAGAACCCTCGCCGCCGCTTCCGCATGGTCGCGGATCTCGCCAAGCGCGCCGAGGACGATAAAAAGCGCAAGAAACTCCAGGGAGATGTACGTGTTGTTCATTTCTTAATCGAAGCGGTGTTCATGGGTGCAACCACCGGAGATATATTAACATTTCTTGAAATGATCGAGTTGCATAGAGAAAAGATACGAGTAGCTCTCTATGTGCAGAAAGCAGCATTGCAATTCATCGATGCTGGTAATAAAGAAGCTGGATACAGTCTTTCCAAAGAAGTTAGTGATGCTGGTTTTGGCATTTCACCTGATGAGCTTGCATCCTTTGCGCGAACCCATGATACCAAGGGTGTGGAAGCACGAGGAGGAGTAGCAGGACTAGCAGGAGATATATCAGTATCCTTGAAAGAAGGCGTTGTTTCAAGCAAGATACCTGTTAGAAAAAACATATATGGCCTCAACCGATTTGTAGAGAAGCCTTCTAAAGGTTTTTGGATGTTTGTCTGGGAAGCCATACAAGATTTAACTCTAATCATCCTTATGGTTTCTGCTGTGGTCTCTATTGGCGTTGGCATTGCAACTGAAGGGTGGCCAAAGGGTATGTATGATGGGTTGGGTATAATATTGAGCATCTTATTGGTTGTGATGGTCACTGCAGTAACTAGAGATGGACGCAGACAGAAGGTTTCTATCTACGATTTGGTGGTTGGAGATATTGTTCATCTTTCAATTGGAGACCAAGTTCCAGCTGATGGCCTTCTTATATCAGGCCACAGCTTACAAATTGATGAATCAAGCTTGTCAGTTACATCTGTTGGTATGAGGACTGAATGGGGACGACTAATGGTTACTCTGAGTGAAGGAGGAGACAATGAGACACCACTGCAGGTGAAACTCAATGGTGTTGCAACCATTATTGGAAAAATTGGTTTGGCTTTTGCAGCTGTCACATTTAGGGGGCTTCCTCTGGCAGTGACACTGAGTCTTGCCTTTGCAATGAAAAAGCTAATGGGTGACAAGGCACTTGTGAGGCATCTTTCAGCATGTGAGACAATGGGCTCTGCTAGTTGCATTTGCACAGATAAGACAGGAACCTTGACAACAAATCATATGTCTATCTTTCTGAATACAACCTCAGAGGTAGTAAAAGGGGAAGATGGGAAAAATACTGTTATAGGTACTCCAACAGAGATGGCTATAGTAGAATTTGGCATGCTTCTGGGAGGTGATTTTAAATCTTTTAGTGAGGAGTATAAGATAGTCATGGTCGAACCATTCAATTCAATCAAAAAAAAGATGTCGGTTCAAGTGGCTCTTCCTGGTACTGATATGTGTCGAGCATTCTGCAAAGGAGCATCAGAAATTATTTTAGGAATGTGTGACAAGGTTGTCAATACTGATGGTGAAGCTATGCCTTTGTCTGAGGAGCAGAGAAAGAAAATATCCGATGTCATAAATGCTGTTGTGGGAATTAAGGATCCTGTGCGCCCTGCCGTGAGGGAAGCCGTGAAGACTTGTTTAGATGCTGGGATTACTGTACGGATGGTCACTGGTGATAATATCAATACAGCTAAAGCCATAGCTAAAAGAGTGGTAATGGCACGATCCTTGCCTTTGGACAAGCACACTTTAGTGAAGCAGTTGAGGAATGTGCATAAAGAAGTAGTGGCAGTAACTGGTGATGGGACTAATGATGCTCCTGCTTTGCACGAGGCAGACATTGGACTCGCTATGGGTATAGCCGGAACAGAGGTTGCAAAAGAAAATGCTGATGTTATCATTATGGATGACAACTTTACAAGTATAGTGAATGTTGCAAGATGGGGTCGTTCAGTTTATATCAACATTCAAAAAGATCAGCTCCTCTCACTGCTGTCCCAGATGCTTTGGGTAAACTTGATTATGGATACTCTTGGTGCTTTGGCACTGGCTACAGAGCCACCGCATGATGGCCTTATGAAAAGACCACCAGTTGGAAGGAATATTAAATTCATCACTGGGATCATGTGGAGGAATATTATCGGTCAGAGTATCTATCAAATTGCTGTCCTTCTGGTTCTTAATTTTTGTGGGAAACGTCTTTTAAAACTCACTGGTCCAAATGCAAGTTCCACTCTCAAGACCTTCATATTCAACTGTTTTGTATTTTGCCAGGTATTCAACGAGATTAACAGCCGTGATATGGAGAAAATCAATGTCTTCAGAGGGATGTTTAATAGTTATACTTTCATGATAGTGATGATCTGCACAGTAGTTTTCCAGATCATAATAGTTGAATTTTTGGGCACTTTTGCTCAAACTGTGCCACTGAGCTGGAAGTTGTGGTTAGCAAGCGTCTTGATTGGAGCCGTAAGTTTACCGGTTGCTGTTGTCTTGAAGTGCATTCCTGTATCATTAAGGAAACAAGCTGCTAAGGATCACAGTGATATCCACGAACCTCTTCTCCGTGGCCCTGAGCTGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009705 GO:0010035 GO:0010941 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055081 GO:0055085 GO:0060548 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098542 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase Hydrolase_3

Protein Analysis

854

Amino Acids

93.56

Weight (kDa)

8.89

Isoelectric Point (pI)

35.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaATP_NAI PF12515 9 - 53 5.7e-22 Ca2+-ATPase N terminal autoinhibitory domain
Cation_ATPase_N PF00690 154 - 220 4.4e-10 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 251 - 300 8.9e-11 P-type ATPase actuator domain
Hydrolase PF00702 390 - 606 1.7e-13 haloacid dehalogenase-like hydrolase
Cation_ATPase PF13246 406 - 519 3.5e-20 P-type ATPase, cytoplasmic domain N
Cation_ATPase_C PF00689 660 - 827 2.9e-38 Cation transporting ATPase, C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015798)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01980 FvH4_7g01980
malus_domestica MD02G1298100.v1.1 MD07G1026000.v1.1
prunus_persica Prupe.2G026200_v2.0.a1
pyrus_communis pycom07g01980
rosa_chinensis RchiOBHm_Chr1g0318981
rosa_laevigata RLG00000030512
rosa_multiflora Rmu_sc0003503.1_g000001 Rmu_sc0006626.1_g000004
rosa_roxburghii Rroxscaffold_4G00328710
rosa_rugosa Rorug01G0026100 Rorug01G0026200
rosa_samantha Rh1AG038000
rosa_wichuraiana Rw1G002930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 976
AasI GACNNNNNNGTC 1 cut(s) 1487
AatII GACGTC 1 cut(s) 45
Acc36I ACCTGC 1 cut(s) 976
AccB7I CCANNNNNTGG 2 cut(s) 851, 2072
AccII CGCG 3 cut(s) 137, 153, 430
AciI CCGC 6 cut(s) 118, 121, 127, 137, 221, 2042
AclWI GGATC 6 cut(s) 147, 1571, 1584, 1698, 2108, 2523
AcoI YGGCCR 1 cut(s) 689
AcsI RAATTY 3 cut(s) 1304, 2087, 2375
AcuI CTGAAG 2 cut(s) 702, 2284
AcyI GRCGYC 1 cut(s) 42
AfaI GTAC 4 cut(s) 195, 1279, 1423, 1638
AfiI CCNNNNNNNGG 6 cut(s) 584, 851, 2048, 2072, 2281, 2557
AflIII ACRYGT 1 cut(s) 196
AgeI ACCGGT 1 cut(s) 2458
AhdI GACNNNNNGTC 2 cut(s) 92, 1080
AjnI CCWGG 3 cut(s) 183, 1417, 2250
AjuI GAANNNNNNNTTGG 4 cut(s) 43, 75, 2191, 2223
AleI CACNNNNGTG 1 cut(s) 1730
AloI GAACNNNNNNTCC 6 cut(s) 801, 825, 833, 857, 2526, 2558
Alw26I GTCTC 7 cut(s) 79, 100, 661, 828, 960, 968, 1142
AlwI GGATC 6 cut(s) 147, 1571, 1584, 1698, 2108, 2523
AlwNI CAGNNNCTG 1 cut(s) 1775
AoxI GGCC 8 cut(s) 69, 90, 553, 689, 853, 868, 2050, 2548
ApeKI GCWGC 3 cut(s) 320, 1040, 2507
ApoI RAATTY 3 cut(s) 1304, 2087, 2375
ArsI GACNNNNNNTTYG 2 cut(s) 1196, 1228
AsiGI ACCGGT 1 cut(s) 2458
Asp700I GAANNNNTTC 1 cut(s) 2538
AspLEI GCGC 6 cut(s) 81, 153, 155, 174, 430, 1587
AspS9I GGNCC 2 cut(s) 2195, 2549
AsuHPI GGTGA 7 cut(s) 396, 1000, 1124, 1337, 1514, 1664, 1790
AvaII GGWCC 1 cut(s) 2195
BaeGI GKGCMC 1 cut(s) 2387
BalI TGGCCA 1 cut(s) 691
BamHI GGATCC 1 cut(s) 1576
BanII GRGCYC 1 cut(s) 1160
BauI CACGAG 2 cut(s) 459, 1808
BbsI GAAGAC 2 cut(s) 1616, 2290
BbvI GCAGC 3 cut(s) 332, 1052, 2494
BceAI ACGGC 5 cut(s) 77, 1577, 1589, 2259, 2432
BcgI CGANNNNNNTGC 2 cut(s) 1425, 1459
BciT130I CCWGG 3 cut(s) 185, 1419, 2252
BciVI GTATCC 3 cut(s) 353, 508, 1999
BcoDI GTCTC 7 cut(s) 79, 100, 661, 828, 960, 968, 1142
BfaI CTAG 3 cut(s) 479, 762, 1164
BfmI CTRYAG 4 cut(s) 753, 983, 1296, 2031
BfoI RGCGCY 1 cut(s) 82
BfuAI ACCTGC 1 cut(s) 976
BfuI GTATCC 3 cut(s) 353, 508, 1999
BisI GCNGC 5 cut(s) 118, 121, 321, 1041, 2508
BlsI GCNGC 5 cut(s) 119, 122, 322, 1042, 2509
Bme1390I CCNGG 3 cut(s) 185, 1419, 2252
Bme18I GGWCC 1 cut(s) 2195
BmeRI GACNNNNNGTC 2 cut(s) 92, 1080
BmgT120I GGNCC 2 cut(s) 2195, 2549
BmiI GGNNCC 3 cut(s) 1192, 1578, 2446
BmrFI CCNGG 3 cut(s) 185, 1419, 2252
BmrI ACTGGG 1 cut(s) 2106
BmsI GCATC 9 cut(s) 328, 376, 428, 738, 1141, 1463, 1612, 1783, 1971
BmuI ACTGGG 1 cut(s) 2106
BpiI GAAGAC 2 cut(s) 1616, 2290
BplI GAGNNNNNCTC 2 cut(s) 288, 320
BpmI CTGGAG 2 cut(s) 103, 167
Bpu10I CCTNAGC 2 cut(s) 2511, 2553
BpuEI CTTGAG 3 cut(s) 46, 94, 2201
Bsa29I ATCGAT 1 cut(s) 336
BsaAI YACGTR 1 cut(s) 197
BsaHI GRCGYC 1 cut(s) 42
BsaI GGTCTC 2 cut(s) 661, 828
BsaJI CCNNGG 5 cut(s) 63, 156, 184, 444, 2545
BsaWI WCCGGW 2 cut(s) 242, 2458
BsaXI ACNNNNNCTCC 6 cut(s) 801, 825, 831, 855, 2526, 2556
Bsc4I CCNNNNNNNGG 6 cut(s) 584, 851, 2048, 2072, 2281, 2557
Bse118I RCCGGY 1 cut(s) 2458
Bse1I ACTGG 6 cut(s) 1654, 1780, 2031, 2069, 2101, 2197
Bse3DI GCAATG 3 cut(s) 323, 672, 1101
BseBI CCWGG 3 cut(s) 185, 1419, 2252
BseCI ATCGAT 1 cut(s) 336
BseDI CCNNGG 5 cut(s) 63, 156, 184, 444, 2545
BseGI GGATG 7 cut(s) 18, 419, 600, 633, 1647, 1891, 2313
BseLI CCNNNNNNNGG 6 cut(s) 584, 851, 2048, 2072, 2281, 2557
BseMI GCAATG 3 cut(s) 323, 672, 1101
BseMII CTCAG 6 cut(s) 944, 1070, 1248, 1512, 2399, 2544
BseNI ACTGG 6 cut(s) 1654, 1780, 2031, 2069, 2101, 2197
BsePI GCGCGC 1 cut(s) 151
BseRI GAGGAG 7 cut(s) 80, 477, 480, 978, 1361, 1538, 1952
BseSI GKGCMC 1 cut(s) 2387
BseXI GCAGC 3 cut(s) 332, 1052, 2494
BseYI CCCAGC 1 cut(s) 1625
BsgI GTGCAG 2 cut(s) 332, 2329
Bsh1236I CGCG 3 cut(s) 137, 153, 430
BshFI GGCC 8 cut(s) 71, 92, 555, 691, 855, 870, 2052, 2550
BshTI ACCGGT 1 cut(s) 2458
BshVI ATCGAT 1 cut(s) 336
BsiSI CCGG 3 cut(s) 243, 1842, 2459
BslFI GGGAC 3 cut(s) 948, 1798, 1960
BslI CCNNNNNNNGG 6 cut(s) 584, 851, 2048, 2072, 2281, 2557
BsmAI GTCTC 7 cut(s) 79, 100, 661, 828, 960, 968, 1142
BsmBI CGTCTC 1 cut(s) 100
BsmFI GGGAC 3 cut(s) 948, 1798, 1960
BsmI GAATGC 2 cut(s) 1439, 2481
BsnI GGCC 8 cut(s) 71, 92, 555, 691, 855, 870, 2052, 2550
Bso31I GGTCTC 2 cut(s) 661, 828
Bsp1286I GDGCHC 2 cut(s) 1160, 2387
Bsp143I GATC 9 cut(s) 139, 270, 1576, 1703, 1954, 2100, 2340, 2361, 2515
BspACI CCGC 6 cut(s) 118, 121, 127, 137, 221, 2042
BspANI GGCC 8 cut(s) 71, 92, 555, 691, 855, 870, 2052, 2550
BspCNI CTCAG 6 cut(s) 945, 1071, 1247, 1513, 2400, 2545
BspDI ATCGAT 1 cut(s) 336
BspFNI CGCG 3 cut(s) 137, 153, 430
BspHI TCATGA 1 cut(s) 2328
BspLI GGNNCC 3 cut(s) 1192, 1578, 2446
BspMAI CTGCAG 2 cut(s) 757, 987
BspMI ACCTGC 1 cut(s) 976
BspPI GGATC 6 cut(s) 147, 1571, 1584, 1698, 2108, 2523
BspQI GCTCTTC 1 cut(s) 1419
BspTNI GGTCTC 2 cut(s) 661, 828
BsrDI GCAATG 3 cut(s) 323, 672, 1101
BsrFI RCCGGY 1 cut(s) 2458
BsrI ACTGG 6 cut(s) 1654, 1780, 2031, 2069, 2101, 2197
BssAI RCCGGY 1 cut(s) 2458
BssECI CCNNGG 5 cut(s) 63, 156, 184, 444, 2545
BssHII GCGCGC 1 cut(s) 151
BssMI GATC 9 cut(s) 139, 270, 1576, 1703, 1954, 2100, 2340, 2361, 2515
BssNI GRCGYC 1 cut(s) 42
BssSI CACGAG 2 cut(s) 459, 1808
BssT1I CCWWGG 1 cut(s) 444
Bst2BI CACGAG 2 cut(s) 459, 1808
Bst2UI CCWGG 3 cut(s) 185, 1419, 2252
Bst4CI ACNGT 7 cut(s) 365, 1270, 1636, 2237, 2350, 2401, 2522
Bst6I CTCTTC 2 cut(s) 1419, 2544
BstACI GRCGYC 1 cut(s) 42
BstAPI GCANNNNNTGC 1 cut(s) 425
BstBAI YACGTR 1 cut(s) 197
BstC8I GCNNGC 5 cut(s) 153, 417, 1313, 2431, 2559
BstDEI CTNAG 7 cut(s) 953, 1079, 1234, 1521, 2408, 2511, 2553
BstDSI CCRYGG 1 cut(s) 2545
BstENI CCTNNNNNAGG 1 cut(s) 582
BstF5I GGATG 7 cut(s) 18, 419, 600, 633, 1647, 1891, 2313
BstFNI CGCG 3 cut(s) 137, 153, 430
BstH2I RGCGCY 1 cut(s) 82
BstHHI GCGC 6 cut(s) 81, 153, 155, 174, 430, 1587
BstKTI GATC 9 cut(s) 142, 273, 1579, 1706, 1957, 2103, 2343, 2364, 2518
BstMAI GTCTC 7 cut(s) 79, 100, 661, 828, 960, 968, 1142
BstMBI GATC 9 cut(s) 139, 270, 1576, 1703, 1954, 2100, 2340, 2361, 2515
BstMWI GCNNNNNNNGC 6 cut(s) 126, 143, 152, 425, 479, 1040
BstNI CCWGG 3 cut(s) 185, 1419, 2252
BstNSI RCATGY 2 cut(s) 1146, 1315
BstSCI CCNGG 3 cut(s) 183, 1417, 2250
BstSFI CTRYAG 4 cut(s) 753, 983, 1296, 2031
BstSLI GKGCMC 1 cut(s) 2387
BstUI CGCG 3 cut(s) 137, 153, 430
BstV1I GCAGC 3 cut(s) 332, 1052, 2494
BstV2I GAAGAC 2 cut(s) 1616, 2290
BstX2I RGATCY 2 cut(s) 139, 1576
BstXI CCANNNNNNTGG 2 cut(s) 451, 2412
BstYI RGATCY 2 cut(s) 139, 1576
Bsu15I ATCGAT 1 cut(s) 336
BsuI GTATCC 3 cut(s) 353, 508, 1999
BsuRI GGCC 8 cut(s) 71, 92, 555, 691, 855, 870, 2052, 2550
BsuTUI ATCGAT 1 cut(s) 336
BtgI CCRYGG 1 cut(s) 2545
BtsCI GGATG 7 cut(s) 18, 419, 600, 633, 1647, 1891, 2313
BtsI GCAGTG 4 cut(s) 750, 980, 1077, 1967
BveI ACCTGC 1 cut(s) 976
Cac8I GCNNGC 5 cut(s) 153, 417, 1313, 2431, 2559
CaiI CAGNNNCTG 1 cut(s) 1775
CciI TCATGA 1 cut(s) 2328
CfoI GCGC 6 cut(s) 81, 153, 155, 174, 430, 1587
Cfr10I RCCGGY 1 cut(s) 2458
Cfr13I GGNCC 2 cut(s) 2195, 2549
ClaI ATCGAT 1 cut(s) 336
CseI GACGC 2 cut(s) 780, 2422
Csp6I GTAC 4 cut(s) 194, 1278, 1422, 1637
CspAI ACCGGT 1 cut(s) 2458
CviAII CATG 9 cut(s) 130, 229, 437, 1143, 1312, 1363, 2045, 2104, 2329
CviQI GTAC 4 cut(s) 194, 1278, 1422, 1637
DdeI CTNAG 7 cut(s) 953, 1079, 1234, 1521, 2408, 2511, 2553
DpnI GATC 9 cut(s) 141, 272, 1578, 1705, 1956, 2102, 2342, 2363, 2517
DpnII GATC 9 cut(s) 139, 270, 1576, 1703, 1954, 2100, 2340, 2361, 2515
DraI TTTAAA 2 cut(s) 1333, 2184
DrdI GACNNNNNNGTC 1 cut(s) 1487
DriI GACNNNNNGTC 2 cut(s) 92, 1080
DseDI GACNNNNNNGTC 1 cut(s) 1487
EaeI YGGCCR 1 cut(s) 689
Eam1104I CTCTTC 2 cut(s) 1419, 2544
Eam1105I GACNNNNNGTC 2 cut(s) 92, 1080
EarI CTCTTC 2 cut(s) 1419, 2544
Eco130I CCWWGG 1 cut(s) 444
Eco147I AGGCCT 1 cut(s) 71
Eco24I GRGCYC 1 cut(s) 1160
Eco31I GGTCTC 2 cut(s) 661, 828
Eco32I GATATC 1 cut(s) 2527
Eco47I GGWCC 1 cut(s) 2195
Eco57I CTGAAG 2 cut(s) 702, 2284
EcoNI CCTNNNNNAGG 1 cut(s) 582
EcoRII CCWGG 3 cut(s) 183, 1417, 2250
EcoRV GATATC 1 cut(s) 2527
EcoT14I CCWWGG 1 cut(s) 444
EcoT38I GRGCYC 1 cut(s) 1160
ErhI CCWWGG 1 cut(s) 444
Esp3I CGTCTC 1 cut(s) 100
FaeI CATG 9 cut(s) 133, 232, 440, 1146, 1315, 1366, 2048, 2107, 2332
FalI AAGNNNNNCTT 2 cut(s) 1398, 1430
FaqI GGGAC 3 cut(s) 948, 1798, 1960
FatI CATG 9 cut(s) 129, 228, 436, 1142, 1311, 1362, 2044, 2103, 2328
FauNDI CATATG 1 cut(s) 1209
Fnu4HI GCNGC 5 cut(s) 118, 121, 321, 1041, 2508
FokI GGATG 7 cut(s) 25, 406, 607, 620, 1654, 1898, 2320
FriOI GRGCYC 1 cut(s) 1160
Fsp4HI GCNGC 5 cut(s) 118, 121, 321, 1041, 2508
FspBI CTAG 3 cut(s) 479, 762, 1164
GlaI GCGC 6 cut(s) 80, 152, 154, 173, 429, 1586
GluI GCNGC 5 cut(s) 118, 121, 321, 1041, 2508
GsaI CCCAGC 1 cut(s) 1629
GsuI CTGGAG 2 cut(s) 103, 167
HaeII RGCGCY 1 cut(s) 82
HaeIII GGCC 8 cut(s) 71, 92, 555, 691, 855, 870, 2052, 2550
HapII CCGG 3 cut(s) 243, 1842, 2459
HgaI GACGC 2 cut(s) 780, 2422
HhaI GCGC 6 cut(s) 81, 153, 155, 174, 430, 1587
Hin1I GRCGYC 1 cut(s) 42
Hin1II CATG 9 cut(s) 133, 232, 440, 1146, 1315, 1366, 2048, 2107, 2332
Hin6I GCGC 6 cut(s) 79, 151, 153, 172, 428, 1585
HinP1I GCGC 6 cut(s) 79, 151, 153, 172, 428, 1585
HindIII AAGCTT 1 cut(s) 895
HinfI GANTC 3 cut(s) 890, 1081, 1824
HpaII CCGG 3 cut(s) 243, 1842, 2459
HphI GGTGA 7 cut(s) 396, 1000, 1124, 1337, 1514, 1664, 1790
Hpy166II GTNNAC 2 cut(s) 1993, 2456
Hpy188I TCNGA 9 cut(s) 64, 954, 1224, 1237, 1459, 1522, 1546, 2127, 2303
Hpy188III TCNNGA 7 cut(s) 106, 263, 2218, 2329, 2359, 2437, 2473
Hpy8I GTNNAC 2 cut(s) 1993, 2456
Hpy99I CGWCG 3 cut(s) 44, 47, 104
HpyCH4III ACNGT 7 cut(s) 365, 1270, 1636, 2237, 2350, 2401, 2522
HpyCH4IV ACGT 3 cut(s) 42, 196, 2176
HpyF10VI GCNNNNNNNGC 6 cut(s) 126, 143, 152, 425, 479, 1040
HpyF3I CTNAG 7 cut(s) 953, 1079, 1234, 1521, 2408, 2511, 2553
HpySE526I ACGT 3 cut(s) 42, 196, 2176
Hsp92I GRCGYC 1 cut(s) 42
Hsp92II CATG 9 cut(s) 133, 232, 440, 1146, 1315, 1366, 2048, 2107, 2332
HspAI GCGC 6 cut(s) 79, 151, 153, 172, 428, 1585
Kzo9I GATC 9 cut(s) 139, 270, 1576, 1703, 1954, 2100, 2340, 2361, 2515
LguI GCTCTTC 1 cut(s) 1419
LmnI GCTCC 5 cut(s) 1451, 1525, 1801, 1965, 2444
Lsp1109I GCAGC 3 cut(s) 332, 1052, 2494
LweI GCATC 9 cut(s) 328, 376, 428, 738, 1141, 1463, 1612, 1783, 1971
MaeI CTAG 3 cut(s) 479, 762, 1164
MaeII ACGT 3 cut(s) 42, 196, 2176
MalI GATC 9 cut(s) 141, 272, 1578, 1705, 1956, 2102, 2342, 2363, 2517
MboI GATC 9 cut(s) 139, 270, 1576, 1703, 1954, 2100, 2340, 2361, 2515
MboII GAAGA 5 cut(s) 1265, 1406, 1621, 2290, 2531
MfeI CAATTG 1 cut(s) 828
MflI RGATCY 2 cut(s) 139, 1576
MhlI GDGCHC 2 cut(s) 1160, 2387
MlsI TGGCCA 1 cut(s) 691
MluNI TGGCCA 1 cut(s) 691
MlyI GAGTC 2 cut(s) 1090, 1818
MmeI TCCRAC 3 cut(s) 787, 1307, 2053
Mox20I TGGCCA 1 cut(s) 691
MroXI GAANNNNTTC 1 cut(s) 2538
MscI TGGCCA 1 cut(s) 691
MslI CAYNNNNRTG 3 cut(s) 638, 1730, 2333
Msp20I TGGCCA 1 cut(s) 691
MspA1I CMGCKG 2 cut(s) 848, 1043
MspI CCGG 3 cut(s) 243, 1842, 2459
MspR9I CCNGG 3 cut(s) 185, 1419, 2252
MunI CAATTG 1 cut(s) 828
Mva1269I GAATGC 2 cut(s) 1439, 2481
MvaI CCWGG 3 cut(s) 185, 1419, 2252
MvnI CGCG 3 cut(s) 137, 153, 430
MwoI GCNNNNNNNGC 6 cut(s) 126, 143, 152, 425, 479, 1040
NdeI CATATG 1 cut(s) 1209
NdeII GATC 9 cut(s) 139, 270, 1576, 1703, 1954, 2100, 2340, 2361, 2515
NlaIII CATG 9 cut(s) 133, 232, 440, 1146, 1315, 1366, 2048, 2107, 2332
NlaIV GGNNCC 3 cut(s) 1192, 1578, 2446
NmeAIII GCCGAG 1 cut(s) 181
NmuCI GTSAC 6 cut(s) 748, 1045, 1072, 1112, 1478, 1645
NspI RCATGY 2 cut(s) 1146, 1315
OliI CACNNNNGTG 1 cut(s) 1730
PaeI GCATGC 1 cut(s) 1315
PagI TCATGA 1 cut(s) 2328
PaqCI CACCTGC 1 cut(s) 976
PauI GCGCGC 1 cut(s) 151
PceI AGGCCT 1 cut(s) 71
PciSI GCTCTTC 1 cut(s) 1419
PctI GAATGC 2 cut(s) 1439, 2481
PdmI GAANNNNTTC 1 cut(s) 2538
PfeI GAWTC 1 cut(s) 890
PflMI CCANNNNNTGG 2 cut(s) 851, 2072
PinAI ACCGGT 1 cut(s) 2458
PkrI GCNGC 5 cut(s) 119, 122, 322, 1042, 2509
PleI GAGTC 2 cut(s) 1089, 1818
PpsI GAGTC 2 cut(s) 1089, 1818
Ppu21I YACGTR 1 cut(s) 197
Psp6I CCWGG 3 cut(s) 183, 1417, 2250
PspFI CCCAGC 1 cut(s) 1625
PspGI CCWGG 3 cut(s) 183, 1417, 2250
PspN4I GGNNCC 3 cut(s) 1192, 1578, 2446
PspPI GGNCC 2 cut(s) 2195, 2549
PsrI GAACNNNNNNTAC 4 cut(s) 186, 218, 424, 456
PstI CTGCAG 2 cut(s) 757, 987
PstNI CAGNNNCTG 1 cut(s) 1775
PsuI RGATCY 2 cut(s) 139, 1576
PteI GCGCGC 1 cut(s) 151
PvuII CAGCTG 2 cut(s) 848, 1043
RsaI GTAC 4 cut(s) 195, 1279, 1423, 1638
RsaNI GTAC 4 cut(s) 194, 1278, 1422, 1637
RseI CAYNNNNRTG 3 cut(s) 638, 1730, 2333
SapI GCTCTTC 1 cut(s) 1419
SatI GCNGC 5 cut(s) 118, 121, 321, 1041, 2508
Sau3AI GATC 9 cut(s) 139, 270, 1576, 1703, 1954, 2100, 2340, 2361, 2515
Sau96I GGNCC 2 cut(s) 2195, 2549
SchI GAGTC 2 cut(s) 1090, 1818
ScrFI CCNGG 3 cut(s) 185, 1419, 2252
SduI GDGCHC 2 cut(s) 1160, 2387
SfaNI GCATC 9 cut(s) 328, 376, 428, 738, 1141, 1463, 1612, 1783, 1971
SfcI CTRYAG 4 cut(s) 753, 983, 1296, 2031
SinI GGWCC 1 cut(s) 2195
SmiMI CAYNNNNRTG 3 cut(s) 638, 1730, 2333
SmlI CTYRAG 3 cut(s) 25, 73, 2216
SmoI CTYRAG 3 cut(s) 25, 73, 2216
SphI GCATGC 1 cut(s) 1315
SseBI AGGCCT 1 cut(s) 71
SsiI CCGC 6 cut(s) 118, 121, 127, 137, 221, 2042
SspI AATATT 3 cut(s) 723, 2083, 2116
SspMI CTAG 3 cut(s) 479, 762, 1164
StuI AGGCCT 1 cut(s) 71
StyD4I CCNGG 3 cut(s) 183, 1417, 2250
StyI CCWWGG 1 cut(s) 444
TaaI ACNGT 7 cut(s) 365, 1270, 1636, 2237, 2350, 2401, 2522
TaiI ACGT 3 cut(s) 45, 199, 2179
TaqI TCGA 7 cut(s) 39, 45, 216, 273, 336, 1368, 1435
TaqII GACCGA 1 cut(s) 2111
TauI GCSGC 2 cut(s) 120, 123
TfiI GAWTC 1 cut(s) 890
TseFI GTSAC 6 cut(s) 748, 1045, 1072, 1112, 1478, 1645
TseI GCWGC 3 cut(s) 320, 1040, 2507
Tsp45I GTSAC 6 cut(s) 748, 1045, 1072, 1112, 1478, 1645
TspGWI ACGGA 3 cut(s) 88, 1654, 2534
Van91I CCANNNNNTGG 2 cut(s) 851, 2072
VpaK11BI GGWCC 1 cut(s) 2195
XagI CCTNNNNNAGG 1 cut(s) 582
XapI RAATTY 3 cut(s) 1304, 2087, 2375
XceI RCATGY 2 cut(s) 1146, 1315
XmnI GAANNNNTTC 1 cut(s) 2538
XspI CTAG 3 cut(s) 479, 762, 1164
ZraI GACGTC 1 cut(s) 43
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.