Rmu_sc0006626.1_g000004

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006626.1
OG0015798
Physical Location & Seq
Reverse (-)
10678 .. 17333
6656 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006626.1_g000004.1.cds

Sequence Viewer

Length: 3117 bp
atgagggagaggatggaggactacttgagaaaaaacttcgacgtcgaaccaaaacgaccttccgacgaggccttgaggcgctggagacaggccgtttccgtcgtcaagaaccctcgccgccgcttccgcatggtcgctgatctcgccaagcgcgccgaggatgataaaaagcgcaagaaactccagacttctattcccttctcagccatctacttttctttgtgtaacacagctggtaataaagaagctggatacagtctttccaaagaagttagtgatgctggttttggcatttcacctgatgagcttgcatcctttgcgcgaacccatgataccaagggtgtggaagcacgaggaggagtagcaggactagcaggagatatatcagtatccttgaaagaaggcgttgtttcaagcaagatacctgttaggcaaaacatatatggcctcaaccgatttgtggagaagccttctaaaggtttttggatgtttgtctgggaagccatacaagatttaactctaatcatccttatggtttctgctgtggtctctattggcgttggcattgcaactgaagggtggccaaagggtatgtatgatgggttgggtataatattgagcatcttattggttgtgatggtcactgcaatcagtgactacaggcaatctttgcaatttaaagaactggataaggaaaaaaaaaatataaagattcaggtaactagagatggacgcagacagaaggtttctatctacgatttggtggttggagatattgttcatctttcaattggagaccaagttccagctgatggccttctcatatcaggccacagcttacaaattgatgaatcaagcttgtcaggtgagagcgagccagtggaaataaatcaaaagagaccttttattcttggaggaaccaaagtgcaagatggctctggtaaaatgctagttacatctgttggtatgaggactgaatggggacgactaatggttactctgagtgaaggaggagacaatgagacaccactgcaggtgaaactcaatggtgttgctaccattattggaaaaattggtttggcttttgcagctgtcacatttgtagttctgactacgaggtacttagttaacaagggaacgcaccataacatgactaactggtctatgaaagatgcatcagcacttttgagttacttttctactgcagttatcataatagttgttgctgttccagaggggcttcctctggcagtgacactgagtcttgcctttgcaatgaaaaagctaatgggtgacaaggcacttgtgaggcatctttcagcatgtgagacgatgggctctgctagttgtatttgcacagataagacgggaaccttgacaacaaatcatatggtggtgaataagatatggatatgtgacgaagccaagactataacgagtggtcagaacaaagatgtattgaagtcagtttctgaaaaagtttataacattcttttgcagtctatctttctgaatacaacctcagaggtagtaaaaggggaagatgggaaaaatactgttataggtactccaacagagatggctatagtagaatttggcatgcttctgggaggtgattttaaatcttttagtgaggagtataagatagttatggtcgaaccattcaattcaatcagaaaaaagatgtcggttcaagtggctcttcctggtagtgatatgtatcgagcattctgcaaaggagcatcagaaattattttaggaatgtgtgacaaggttgtcaatactgatggtgaagctatgcctttgtctgaggagcagagaaagaaaatatctgatgtcataaatggttttgcttgtgaagctttaagaactctatgcgtagctttcaaggatatagaggacccttctagtgcaaaaagtattcctgaagataaatatacattaatagctgttgtgggaattaaggatcctgtgcgccctggcgtgagggaagccgtgaagacttgtttagatgctgggattactgtacggatggtcactggtgataatatcaatacagctaaagccatagctaaagagtgtggcattttgacagaagatggtttagctatagaagggccagatttccgaaaaatgagccaacaggagatggcagaaattatcccaaaactgcaggtaatggcacgatccttgcctttggacaagcacactttagtgaagcagttgaggaatgtgcataaagaagtagtggcagtaactggtgatgggactaatgatgctcctgctttgcacgaggcagacattggactcgctatgggtatagccggaacagaggttgcaaaagaaaatgctgatgttatcattatggatgacaactttacaagtatagtgaatgttgcaagatggggtcgttcagtttatatcaacattcaaaagtttgttcagttccagttaacggttaatattgtcgctctgatgctcaatttcgtatccgcatgcgtctcaggatcagctcctctcactgctgtccagatgctttgggtaaacttgattatggatactcttggtgctttggcactggctacagagccaccgcatgatggccttatgaaaagaccaccagttggaaggaatattaaattcatcactgggatcatgtggaggaatattatcggtcagagtatctatcaaattgctgtccttctggttcttaatttttgtgggaaacgtcttttaaaactcaccggtccaaatgcaagttccattctcaagaccttcatattcaactgttttgtattttgccaggtattcaacgagattaacagccgtgatatggagaaaatcaatgtgttcagagggatgtttaatagttatactttcatgatagtgatgatctgcacagtagttttccagatcataatagttgaatttttgggcacttttgctcaaactgtgccactgagctggaagttgtggttagcaagcgtcttgattggagccgtaagtttaccggtttctgttgtcttgaagtgcattcctgtatcattaaggaaacaagctgctaaggatcacagtgatatccacgaacctcttctccgtggccctgagctggcttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009705 GO:0010035 GO:0010941 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055081 GO:0055085 GO:0060548 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098542 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase Hydrolase_3

Protein Analysis

1038

Amino Acids

113.77

Weight (kDa)

8.59

Isoelectric Point (pI)

38.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015798)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01980 FvH4_7g01980
malus_domestica MD02G1298100.v1.1 MD07G1026000.v1.1
prunus_persica Prupe.2G026200_v2.0.a1
pyrus_communis pycom07g01980
rosa_chinensis RchiOBHm_Chr1g0318981
rosa_laevigata RLG00000030512
rosa_multiflora Rmu_sc0003503.1_g000001 Rmu_sc0006626.1_g000004
rosa_roxburghii Rroxscaffold_4G00328710
rosa_rugosa Rorug01G0026100 Rorug01G0026200
rosa_samantha Rh1AG038000
rosa_wichuraiana Rw1G002930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1485
AarI CACCTGC 1 cut(s) 1024
AasI GACNNNNNNGTC 2 cut(s) 1159, 1775
AatII GACGTC 1 cut(s) 45
Acc36I ACCTGC 2 cut(s) 1024, 2164
AccB7I CCANNNNNTGG 2 cut(s) 812, 2624
AccII CGCG 2 cut(s) 153, 322
AciI CCGC 5 cut(s) 118, 121, 127, 2493, 2594
AclWI GGATC 6 cut(s) 1961, 1974, 2181, 2515, 2660, 3075
AcoI YGGCCR 1 cut(s) 581
AcsI RAATTY 3 cut(s) 1592, 2639, 2927
AcuI CTGAAG 2 cut(s) 594, 1947
AcyI GRCGYC 1 cut(s) 42
AfaI GTAC 3 cut(s) 1121, 1567, 2028
AfiI CCNNNNNNNGG 8 cut(s) 460, 476, 812, 2455, 2600, 2624, 2833, 3109
AgeI ACCGGT 2 cut(s) 2744, 3010
AhdI GACNNNNNGTC 1 cut(s) 1260
AjnI CCWGG 3 cut(s) 1705, 1978, 2802
AjuI GAANNNNNNNTTGG 4 cut(s) 43, 75, 2743, 2775
AleI CACNNNNGTG 1 cut(s) 2213
AloI GAACNNNNNNTCC 6 cut(s) 762, 786, 794, 818, 3078, 3110
Alw26I GTCTC 8 cut(s) 79, 553, 789, 892, 1008, 1016, 1322, 2506
AlwI GGATC 6 cut(s) 1961, 1974, 2181, 2515, 2660, 3075
AlwNI CAGNNNCTG 2 cut(s) 1472, 2258
AoxI GGCC 9 cut(s) 69, 90, 445, 581, 814, 829, 2117, 2602, 3100
ApeKI GCWGC 2 cut(s) 1088, 3059
ApoI RAATTY 3 cut(s) 1592, 2639, 2927
AseI ATTAAT 1 cut(s) 1943
AsiGI ACCGGT 2 cut(s) 2744, 3010
Asp700I GAANNNNTTC 1 cut(s) 3090
AspLEI GCGC 6 cut(s) 81, 153, 155, 174, 322, 1977
AspS9I GGNCC 4 cut(s) 1900, 2117, 2747, 3101
AvaII GGWCC 2 cut(s) 1900, 2747
BaeGI GKGCMC 1 cut(s) 2939
BalI TGGCCA 1 cut(s) 583
BamHI GGATCC 1 cut(s) 1966
BanII GRGCYC 1 cut(s) 1340
BauI CACGAG 2 cut(s) 351, 2291
BbsI GAAGAC 1 cut(s) 2006
BbvI GCAGC 2 cut(s) 1100, 3046
BceAI ACGGC 4 cut(s) 77, 1979, 2811, 2984
BcgI CGANNNNNNTGC 2 cut(s) 1713, 1747
BciT130I CCWGG 3 cut(s) 1707, 1980, 2804
BciVI GTATCC 4 cut(s) 245, 400, 2500, 2551
BcoDI GTCTC 8 cut(s) 79, 553, 789, 892, 1008, 1016, 1322, 2506
BfaI CTAG 5 cut(s) 371, 723, 950, 1344, 1908
BfmI CTRYAG 7 cut(s) 658, 1031, 1203, 1584, 2109, 2171, 2583
BfoI RGCGCY 1 cut(s) 82
BfuAI ACCTGC 2 cut(s) 1024, 2164
BfuI GTATCC 4 cut(s) 245, 400, 2500, 2551
BisI GCNGC 4 cut(s) 118, 121, 1089, 3060
BlsI GCNGC 4 cut(s) 119, 122, 1090, 3061
Bme1390I CCNGG 3 cut(s) 1707, 1980, 2804
Bme18I GGWCC 2 cut(s) 1900, 2747
BmeRI GACNNNNNGTC 1 cut(s) 1260
BmgT120I GGNCC 4 cut(s) 1900, 2117, 2747, 3101
BmiI GGNNCC 5 cut(s) 919, 1372, 1902, 1968, 2998
BmrFI CCNGG 3 cut(s) 1707, 1980, 2804
BmrI ACTGGG 1 cut(s) 2658
BmuI ACTGGG 1 cut(s) 2658
BpiI GAAGAC 1 cut(s) 2006
BpmI CTGGAG 2 cut(s) 103, 167
Bpu10I CCTNAGC 2 cut(s) 3063, 3105
BpuEI CTTGAG 3 cut(s) 46, 94, 2753
BsaBI GATNNNNATC 1 cut(s) 1719
BsaHI GRCGYC 1 cut(s) 42
BsaI GGTCTC 3 cut(s) 553, 789, 892
BsaJI CCNNGG 4 cut(s) 156, 336, 1978, 3097
BsaWI WCCGGW 2 cut(s) 2744, 3010
BsaXI ACNNNNNCTCC 6 cut(s) 762, 786, 792, 816, 3078, 3108
Bsc4I CCNNNNNNNGG 8 cut(s) 460, 476, 812, 2455, 2600, 2624, 2833, 3109
Bse118I RCCGGY 2 cut(s) 2744, 3010
Bse1I ACTGG 9 cut(s) 690, 878, 1163, 2044, 2263, 2449, 2583, 2621, 2653
Bse3DI GCAATG 2 cut(s) 564, 1281
Bse8I GATNNNNATC 1 cut(s) 1719
BseBI CCWGG 3 cut(s) 1707, 1980, 2804
BseDI CCNNGG 4 cut(s) 156, 336, 1978, 3097
BseGI GGATG 8 cut(s) 18, 166, 311, 492, 525, 2037, 2374, 2865
BseJI GATNNNNATC 1 cut(s) 1719
BseLI CCNNNNNNNGG 8 cut(s) 460, 476, 812, 2455, 2600, 2624, 2833, 3109
BseMI GCAATG 2 cut(s) 564, 1281
BseMII CTCAG 8 cut(s) 216, 992, 1250, 1536, 1800, 2517, 2951, 3096
BseNI ACTGG 9 cut(s) 690, 878, 1163, 2044, 2263, 2449, 2583, 2621, 2653
BsePI GCGCGC 1 cut(s) 151
BseRI GAGGAG 6 cut(s) 369, 372, 1026, 1649, 1826, 2505
BseSI GKGCMC 1 cut(s) 2939
BseXI GCAGC 2 cut(s) 1100, 3046
BseYI CCCAGC 1 cut(s) 2015
BsgI GTGCAG 1 cut(s) 2881
Bsh1236I CGCG 2 cut(s) 153, 322
BshFI GGCC 9 cut(s) 71, 92, 447, 583, 816, 831, 2119, 2604, 3102
BshTI ACCGGT 2 cut(s) 2744, 3010
BsiSI CCGG 3 cut(s) 2325, 2745, 3011
BslFI GGGAC 2 cut(s) 996, 2281
BslI CCNNNNNNNGG 8 cut(s) 460, 476, 812, 2455, 2600, 2624, 2833, 3109
BsmAI GTCTC 8 cut(s) 79, 553, 789, 892, 1008, 1016, 1322, 2506
BsmBI CGTCTC 2 cut(s) 1322, 2506
BsmFI GGGAC 2 cut(s) 996, 2281
BsmI GAATGC 2 cut(s) 1727, 3033
BsnI GGCC 9 cut(s) 71, 92, 447, 583, 816, 831, 2119, 2604, 3102
Bso31I GGTCTC 3 cut(s) 553, 789, 892
Bsp1286I GDGCHC 2 cut(s) 1340, 2939
Bsp143I GATC 8 cut(s) 139, 1966, 2186, 2507, 2652, 2892, 2913, 3067
BspACI CCGC 5 cut(s) 118, 121, 127, 2493, 2594
BspANI GGCC 9 cut(s) 71, 92, 447, 583, 816, 831, 2119, 2604, 3102
BspCNI CTCAG 8 cut(s) 215, 993, 1251, 1535, 1801, 2516, 2952, 3097
BspFNI CGCG 2 cut(s) 153, 322
BspHI TCATGA 1 cut(s) 2880
BspLI GGNNCC 5 cut(s) 919, 1372, 1902, 1968, 2998
BspMAI CTGCAG 3 cut(s) 1035, 1207, 2175
BspMI ACCTGC 2 cut(s) 1024, 2164
BspPI GGATC 6 cut(s) 1961, 1974, 2181, 2515, 2660, 3075
BspQI GCTCTTC 1 cut(s) 1707
BspTNI GGTCTC 3 cut(s) 553, 789, 892
BsrDI GCAATG 2 cut(s) 564, 1281
BsrFI RCCGGY 2 cut(s) 2744, 3010
BsrI ACTGG 9 cut(s) 690, 878, 1163, 2044, 2263, 2449, 2583, 2621, 2653
BssAI RCCGGY 2 cut(s) 2744, 3010
BssECI CCNNGG 4 cut(s) 156, 336, 1978, 3097
BssHII GCGCGC 1 cut(s) 151
BssMI GATC 8 cut(s) 139, 1966, 2186, 2507, 2652, 2892, 2913, 3067
BssNI GRCGYC 1 cut(s) 42
BssSI CACGAG 2 cut(s) 351, 2291
BssT1I CCWWGG 1 cut(s) 336
Bst2BI CACGAG 2 cut(s) 351, 2291
Bst2UI CCWGG 3 cut(s) 1707, 1980, 2804
Bst4CI ACNGT 8 cut(s) 257, 1558, 2026, 2458, 2789, 2902, 2953, 3074
Bst6I CTCTTC 2 cut(s) 1707, 3096
BstACI GRCGYC 1 cut(s) 42
BstAPI GCANNNNNTGC 2 cut(s) 317, 670
BstC8I GCNNGC 7 cut(s) 153, 309, 875, 1601, 2497, 2983, 3111
BstDSI CCRYGG 1 cut(s) 3097
BstENI CCTNNNNNAGG 1 cut(s) 474
BstF5I GGATG 8 cut(s) 18, 166, 311, 492, 525, 2037, 2374, 2865
BstFNI CGCG 2 cut(s) 153, 322
BstH2I RGCGCY 1 cut(s) 82
BstHHI GCGC 6 cut(s) 81, 153, 155, 174, 322, 1977
BstKTI GATC 8 cut(s) 142, 1969, 2189, 2510, 2655, 2895, 2916, 3070
BstMAI GTCTC 8 cut(s) 79, 553, 789, 892, 1008, 1016, 1322, 2506
BstMBI GATC 8 cut(s) 139, 1966, 2186, 2507, 2652, 2892, 2913, 3067
BstMWI GCNNNNNNNGC 8 cut(s) 126, 143, 152, 317, 371, 670, 1088, 1859
BstNI CCWGG 3 cut(s) 1707, 1980, 2804
BstNSI RCATGY 3 cut(s) 1326, 1603, 2499
BstSCI CCNGG 3 cut(s) 1705, 1978, 2802
BstSFI CTRYAG 7 cut(s) 658, 1031, 1203, 1584, 2109, 2171, 2583
BstSLI GKGCMC 1 cut(s) 2939
BstUI CGCG 2 cut(s) 153, 322
BstV1I GCAGC 2 cut(s) 1100, 3046
BstV2I GAAGAC 1 cut(s) 2006
BstX2I RGATCY 1 cut(s) 1966
BstXI CCANNNNNNTGG 2 cut(s) 343, 2964
BstYI RGATCY 1 cut(s) 1966
BsuI GTATCC 4 cut(s) 245, 400, 2500, 2551
BsuRI GGCC 9 cut(s) 71, 92, 447, 583, 816, 831, 2119, 2604, 3102
BtgI CCRYGG 1 cut(s) 3097
BtsCI GGATG 8 cut(s) 18, 166, 311, 492, 525, 2037, 2374, 2865
BtsI GCAGTG 4 cut(s) 642, 1028, 1257, 2520
BveI ACCTGC 2 cut(s) 1024, 2164
Cac8I GCNNGC 7 cut(s) 153, 309, 875, 1601, 2497, 2983, 3111
CaiI CAGNNNCTG 2 cut(s) 1472, 2258
CciI TCATGA 1 cut(s) 2880
CfoI GCGC 6 cut(s) 81, 153, 155, 174, 322, 1977
Cfr10I RCCGGY 2 cut(s) 2744, 3010
Cfr13I GGNCC 4 cut(s) 1900, 2117, 2747, 3101
CseI GACGC 3 cut(s) 741, 2488, 2974
Csp6I GTAC 3 cut(s) 1120, 1566, 2027
CspAI ACCGGT 2 cut(s) 2744, 3010
CviAII CATG 9 cut(s) 130, 329, 1150, 1323, 1600, 2496, 2597, 2656, 2881
CviQI GTAC 3 cut(s) 1120, 1566, 2027
DpnI GATC 8 cut(s) 141, 1968, 2188, 2509, 2654, 2894, 2915, 3069
DpnII GATC 8 cut(s) 139, 1966, 2186, 2507, 2652, 2892, 2913, 3067
DraI TTTAAA 3 cut(s) 679, 1621, 2736
DrdI GACNNNNNNGTC 2 cut(s) 1159, 1775
DriI GACNNNNNGTC 1 cut(s) 1260
DseDI GACNNNNNNGTC 2 cut(s) 1159, 1775
EaeI YGGCCR 1 cut(s) 581
Eam1104I CTCTTC 2 cut(s) 1707, 3096
Eam1105I GACNNNNNGTC 1 cut(s) 1260
EarI CTCTTC 2 cut(s) 1707, 3096
Eco130I CCWWGG 1 cut(s) 336
Eco147I AGGCCT 1 cut(s) 71
Eco24I GRGCYC 1 cut(s) 1340
Eco31I GGTCTC 3 cut(s) 553, 789, 892
Eco32I GATATC 1 cut(s) 3079
Eco47I GGWCC 2 cut(s) 1900, 2747
Eco57I CTGAAG 2 cut(s) 594, 1947
EcoNI CCTNNNNNAGG 1 cut(s) 474
EcoO109I RGGNCCY 1 cut(s) 1900
EcoRII CCWGG 3 cut(s) 1705, 1978, 2802
EcoRV GATATC 1 cut(s) 3079
EcoT14I CCWWGG 1 cut(s) 336
EcoT22I ATGCAT 1 cut(s) 1177
EcoT38I GRGCYC 1 cut(s) 1340
ErhI CCWWGG 1 cut(s) 336
Esp3I CGTCTC 2 cut(s) 1322, 2506
FaeI CATG 9 cut(s) 133, 332, 1153, 1326, 1603, 2499, 2600, 2659, 2884
FalI AAGNNNNNCTT 2 cut(s) 1686, 1718
FaqI GGGAC 2 cut(s) 996, 2281
FatI CATG 9 cut(s) 129, 328, 1149, 1322, 1599, 2495, 2596, 2655, 2880
FauNDI CATATG 1 cut(s) 1389
Fnu4HI GCNGC 4 cut(s) 118, 121, 1089, 3060
FokI GGATG 8 cut(s) 25, 173, 298, 499, 512, 2044, 2381, 2872
FriOI GRGCYC 1 cut(s) 1340
Fsp4HI GCNGC 4 cut(s) 118, 121, 1089, 3060
FspBI CTAG 5 cut(s) 371, 723, 950, 1344, 1908
GlaI GCGC 6 cut(s) 80, 152, 154, 173, 321, 1976
GluI GCNGC 4 cut(s) 118, 121, 1089, 3060
GsaI CCCAGC 1 cut(s) 2019
GsuI CTGGAG 2 cut(s) 103, 167
HaeII RGCGCY 1 cut(s) 82
HaeIII GGCC 9 cut(s) 71, 92, 447, 583, 816, 831, 2119, 2604, 3102
HapII CCGG 3 cut(s) 2325, 2745, 3011
HgaI GACGC 3 cut(s) 741, 2488, 2974
HhaI GCGC 6 cut(s) 81, 153, 155, 174, 322, 1977
Hin1I GRCGYC 1 cut(s) 42
Hin1II CATG 9 cut(s) 133, 332, 1153, 1326, 1603, 2499, 2600, 2659, 2884
Hin6I GCGC 6 cut(s) 79, 151, 153, 172, 320, 1975
HinP1I GCGC 6 cut(s) 79, 151, 153, 172, 320, 1975
HincII GTYRAC 2 cut(s) 1129, 2454
HindII GTYRAC 2 cut(s) 1129, 2454
HindIII AAGCTT 2 cut(s) 856, 1860
HinfI GANTC 4 cut(s) 712, 851, 1261, 2307
HpaI GTTAAC 2 cut(s) 1129, 2454
HpaII CCGG 3 cut(s) 2325, 2745, 3011
Hpy166II GTNNAC 4 cut(s) 1129, 2454, 2545, 3008
Hpy8I GTNNAC 4 cut(s) 1129, 2454, 2545, 3008
Hpy99I CGWCG 4 cut(s) 44, 47, 68, 104
HpyCH4III ACNGT 8 cut(s) 257, 1558, 2026, 2458, 2789, 2902, 2953, 3074
HpyCH4IV ACGT 2 cut(s) 42, 2728
HpyF10VI GCNNNNNNNGC 8 cut(s) 126, 143, 152, 317, 371, 670, 1088, 1859
HpySE526I ACGT 2 cut(s) 42, 2728
Hsp92I GRCGYC 1 cut(s) 42
Hsp92II CATG 9 cut(s) 133, 332, 1153, 1326, 1603, 2499, 2600, 2659, 2884
HspAI GCGC 6 cut(s) 79, 151, 153, 172, 320, 1975
KspAI GTTAAC 2 cut(s) 1129, 2454
Kzo9I GATC 8 cut(s) 139, 1966, 2186, 2507, 2652, 2892, 2913, 3067
LguI GCTCTTC 1 cut(s) 1707
LmnI GCTCC 5 cut(s) 1739, 1813, 2284, 2518, 2996
Lsp1109I GCAGC 2 cut(s) 1100, 3046
MaeI CTAG 5 cut(s) 371, 723, 950, 1344, 1908
MaeII ACGT 2 cut(s) 42, 2728
MalI GATC 8 cut(s) 141, 1968, 2188, 2509, 2654, 2894, 2915, 3069
MboI GATC 8 cut(s) 139, 1966, 2186, 2507, 2652, 2892, 2913, 3067
MboII GAAGA 6 cut(s) 1553, 1694, 1940, 2011, 2108, 3083
MfeI CAATTG 1 cut(s) 789
MflI RGATCY 1 cut(s) 1966
MhlI GDGCHC 2 cut(s) 1340, 2939
MlsI TGGCCA 1 cut(s) 583
MluNI TGGCCA 1 cut(s) 583
MlyI GAGTC 2 cut(s) 1270, 2301
MmeI TCCRAC 4 cut(s) 87, 748, 1595, 2605
Mox20I TGGCCA 1 cut(s) 583
Mph1103I ATGCAT 1 cut(s) 1177
MroXI GAANNNNTTC 1 cut(s) 3090
MscI TGGCCA 1 cut(s) 583
MslI CAYNNNNRTG 3 cut(s) 530, 2213, 2885
Msp20I TGGCCA 1 cut(s) 583
MspA1I CMGCKG 3 cut(s) 233, 809, 1091
MspI CCGG 3 cut(s) 2325, 2745, 3011
MspR9I CCNGG 3 cut(s) 1707, 1980, 2804
MunI CAATTG 1 cut(s) 789
Mva1269I GAATGC 2 cut(s) 1727, 3033
MvaI CCWGG 3 cut(s) 1707, 1980, 2804
MvnI CGCG 2 cut(s) 153, 322
MwoI GCNNNNNNNGC 8 cut(s) 126, 143, 152, 317, 371, 670, 1088, 1859
NdeI CATATG 1 cut(s) 1389
NdeII GATC 8 cut(s) 139, 1966, 2186, 2507, 2652, 2892, 2913, 3067
NlaIII CATG 9 cut(s) 133, 332, 1153, 1326, 1603, 2499, 2600, 2659, 2884
NlaIV GGNNCC 5 cut(s) 919, 1372, 1902, 1968, 2998
NmeAIII GCCGAG 1 cut(s) 181
NmuCI GTSAC 8 cut(s) 640, 653, 1093, 1252, 1292, 1415, 1766, 2035
NsiI ATGCAT 1 cut(s) 1177
NspI RCATGY 3 cut(s) 1326, 1603, 2499
OliI CACNNNNGTG 1 cut(s) 2213
PaeI GCATGC 2 cut(s) 1603, 2499
PagI TCATGA 1 cut(s) 2880
PaqCI CACCTGC 1 cut(s) 1024
PauI GCGCGC 1 cut(s) 151
PceI AGGCCT 1 cut(s) 71
PciSI GCTCTTC 1 cut(s) 1707
PctI GAATGC 2 cut(s) 1727, 3033
PdmI GAANNNNTTC 1 cut(s) 3090
PfeI GAWTC 2 cut(s) 712, 851
PflMI CCANNNNNTGG 2 cut(s) 812, 2624
PinAI ACCGGT 2 cut(s) 2744, 3010
PkrI GCNGC 4 cut(s) 119, 122, 1090, 3061
PleI GAGTC 2 cut(s) 1269, 2301
PpsI GAGTC 2 cut(s) 1269, 2301
PpuMI RGGWCCY 1 cut(s) 1900
PshBI ATTAAT 1 cut(s) 1943
PsiI TTATAA 1 cut(s) 1485
Psp5II RGGWCCY 1 cut(s) 1900
Psp6I CCWGG 3 cut(s) 1705, 1978, 2802
PspFI CCCAGC 1 cut(s) 2015
PspGI CCWGG 3 cut(s) 1705, 1978, 2802
PspN4I GGNNCC 5 cut(s) 919, 1372, 1902, 1968, 2998
PspPI GGNCC 4 cut(s) 1900, 2117, 2747, 3101
PspPPI RGGWCCY 1 cut(s) 1900
PsrI GAACNNNNNNTAC 2 cut(s) 316, 348
PstI CTGCAG 3 cut(s) 1035, 1207, 2175
PstNI CAGNNNCTG 2 cut(s) 1472, 2258
PsuI RGATCY 1 cut(s) 1966
PteI GCGCGC 1 cut(s) 151
PvuII CAGCTG 3 cut(s) 233, 809, 1091
RsaI GTAC 3 cut(s) 1121, 1567, 2028
RsaNI GTAC 3 cut(s) 1120, 1566, 2027
RseI CAYNNNNRTG 3 cut(s) 530, 2213, 2885
SapI GCTCTTC 1 cut(s) 1707
SatI GCNGC 4 cut(s) 118, 121, 1089, 3060
Sau3AI GATC 8 cut(s) 139, 1966, 2186, 2507, 2652, 2892, 2913, 3067
Sau96I GGNCC 4 cut(s) 1900, 2117, 2747, 3101
SchI GAGTC 2 cut(s) 1270, 2301
ScrFI CCNGG 3 cut(s) 1707, 1980, 2804
SduI GDGCHC 2 cut(s) 1340, 2939
SfcI CTRYAG 7 cut(s) 658, 1031, 1203, 1584, 2109, 2171, 2583
SinI GGWCC 2 cut(s) 1900, 2747
SmiMI CAYNNNNRTG 3 cut(s) 530, 2213, 2885
SmlI CTYRAG 3 cut(s) 25, 73, 2768
SmoI CTYRAG 3 cut(s) 25, 73, 2768
SphI GCATGC 2 cut(s) 1603, 2499
SseBI AGGCCT 1 cut(s) 71
SsiI CCGC 5 cut(s) 118, 121, 127, 2493, 2594
SspI AATATT 4 cut(s) 615, 2464, 2635, 2668
SspMI CTAG 5 cut(s) 371, 723, 950, 1344, 1908
StuI AGGCCT 1 cut(s) 71
StyD4I CCNGG 3 cut(s) 1705, 1978, 2802
StyI CCWWGG 1 cut(s) 336
TaaI ACNGT 8 cut(s) 257, 1558, 2026, 2458, 2789, 2902, 2953, 3074
TaiI ACGT 2 cut(s) 45, 2731
TaqI TCGA 4 cut(s) 39, 45, 1656, 1723
TaqII GACCGA 1 cut(s) 2663
TauI GCSGC 2 cut(s) 120, 123
TfiI GAWTC 2 cut(s) 712, 851
TseFI GTSAC 8 cut(s) 640, 653, 1093, 1252, 1292, 1415, 1766, 2035
TseI GCWGC 2 cut(s) 1088, 3059
Tsp45I GTSAC 8 cut(s) 640, 653, 1093, 1252, 1292, 1415, 1766, 2035
TspDTI ATGAA 8 cut(s) 770, 864, 1181, 1292, 2624, 2632, 2767, 2869
TspGWI ACGGA 3 cut(s) 88, 2044, 3086
Van91I CCANNNNNTGG 2 cut(s) 812, 2624
VpaK11BI GGWCC 2 cut(s) 1900, 2747
VspI ATTAAT 1 cut(s) 1943
XagI CCTNNNNNAGG 1 cut(s) 474
XapI RAATTY 3 cut(s) 1592, 2639, 2927
XceI RCATGY 3 cut(s) 1326, 1603, 2499
XmnI GAANNNNTTC 1 cut(s) 3090
XspI CTAG 5 cut(s) 371, 723, 950, 1344, 1908
ZraI GACGTC 1 cut(s) 43
Zsp2I ATGCAT 1 cut(s) 1177
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.