RLG00000030512

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0015798
Physical Location & Seq
Reverse (-)
62694780 .. 62701499
6720 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030512

Sequence Viewer

Length: 3126 bp
ATGAGGGAGAGGATGGAGGACTACTTGAGAAAAAACTTCGAAGTCGAACCAAAACGACCTTCCGACGAGGCCTTGAGGCGCTGGAGACAGGCCGTCTCCGTCGTCAAGAACCCTCGCCGCCGCTTCCGCATGGTCGCTGATCTCGCCAAGCGCGCCGAGGACGATAAAAAGCGCAAGAAACTCCAGGAAAAGATACGAGTAGCTCTCTATGTGCAGAAAGCAGCATTGCAATTCATCGATGCTGGTAATAAAGAAGCTGGATACAGTCTTTCCAAAGAAGTTAGTAATGCTGGTTTTGGCATTTCACCTGATGAGCTTGCCTCCTTTGCGCGAACCCATGATACCAAGGGTGTGGAAGCACGAGGAGGAGTAGCAGGACTAGCAGGAGATATATCAGTATCCTTGAAAGAAGGCGTTGTTTCAAGCAAGATACCTGTTAGGCAAAACATATATGGCCTTAACCGATTTGTGGAGAAGCCTTCTAAAGGTTTTTGGATGTTTGTCTGGGAAGCCATACAAGATTTAACTCTAATCATCCTTATGGTTTCTGCTGTGGTCTCTATTGGAGTTGGCATTGCAACTGAAGGGTGGCCAAAGGGTATGTATGATGGGTTGGGTATAATATTGAGCATCTTATTGGTTGTGATGGTCACTGCAATCAGTGACTACAGGCAATCTTTGCAATTTAAAGAACTGGATAAGGAGAAAAAAAATATAAAGATTCAGGTAACTAGAGATGGACGCAGACAGAAGGTTTCTATCTACGATTTGGTGGTTGGAGATATTGTTCATCTTTCAATTGGAGACCAAGTTCCAGCTGATGGCCTTCTTATATCAGGCCACAGCTTACAAATTGATGAATCAAGCTTGTCAGGTGAGAGCGAGCCAGTGGACATAAATCAAAATAGACCTTTTATTCTTGGAGGAACCAAAGTGCAAGATGGCTCTGGTAAAATGCTAGTTACATCTGTTGGTATGAGGACTGAATGGGGACGACTAATGGTTACTCTGAGTGAAGGAGGAGACAATGAGACACCACTGCAGGTGAAACTCAATGGTGTTGCAACCATTATTGGAAAAATTGGTTTGGCTTTTGCAGCTGTCACATTTGTAGTTCTGACTACGAGGTACTTAGTTAACAAGGGAACGCACCATAACATGACTAACTGGTCTATGAAAGATGCATCTGCACTTTTGAGTTACTTTTCTACTGCAGTTATCATAATAGTTGTTGCTGTTCCAGAGGGGCTTCCTCTGGCAGTGACACTGAGTCTTGCCTTTGCAATGAAAAAGCTAATGGGTGACAAGGCACTTGTGAGGCATCTTTCAGCATGTGAGACGATGGGCTCCGCTAGTTGTATTTGCACAGATAAGACGGGAACCTTGACAACAAATCATATGGTGGTGAATAAGATATGGATCTGTGACGAAGCCAAGACTATAACGAGTGCTCAGAACAAAGATGTATTGAAGTCAGTTTCTGAAAAGGTTTATAACATTCTTTTGCAGTCTATCTTTCTGAATACAACCTCAGAGGTAGTAAAAGGGGAAGATGGGAAAAATACTGTTATAGGTACTCCAACAGAGATGGCTATAGTAGAATTTGGCATGCTTCTGGGAGGTGATTTTAAATCTTTTAGTGAGGAGTATAAGATAGTTATGGTCGAACCATTCAATTCAATCAGAAAAAAGATGTCGGTTCAAGTGGCTCGTCCTGGTAGTGATATGTGTCGAGCATTCTGCAAAGGAGCATCGGAAATTATTTTAGGAATGTGTGACAAGGTTGTCAATACTGATGGTGAAGCTATGCCTTTGTCTGAGGAGCAGAGAAAGAAAATATCTGATGTCATAAATGGTTTTGCTTGTGAAGCTTTAAGAACTCTATGCGTAGCGTTCAAGGATATAGAGGACCCTTCTAGTGCAAAAAGTATTCCTGAAGATAAATATACATTAATAGCCGTTGTGGGAATTAAGGATCCTGTGCGCCCTGCCGTGAGGGAAGCCGTGAAGACTTGTTTAGATGCTGGGATTACTGTACGGATGGTCACTGGTGATAATATCAATACAGCTAAAGCCATAGCTAAAGAGTGTGGCATTTTGACAGAAGATGGTTTAGCTATAGAGGGGCCAGATTTCAGAAAAATGAGCCAACAGGAGATGGCAGAAATAATCCCAAAACTGCAGGTGATGGCACGATCCTTGCCTTTGGACAAGCACACTTTAGTGAAGCAGTTGAGGAATGTGCATAAAGAAGTAGTGGCAGTCACTGGTGATGGGACTAATGATGCTCCTGCTTTGCACGAGGCAGACATTGGACTCGCTATGGGTATAGCCGGAACAGAGGTTGCAAAAGAAAATGCTGATGTTATCATTATGGATGACAACTTTACAAGTATAGTGAATGTTGCAAGATGGGGTCGTTCAGTTTATATCAACATTCAAAAGTTTGTTCAGTTCCAGTTAACGGTTAATATTGTCGCTCTGATGCTCAATTTCGTATCAGCATGCGTCTCAGGATCAGCTCCTCTCACTGCTGTCCAGATGCTTTGGGTAAACTTGATTATGGATACGCTTGGCGCTTTGGCACTGGCTACAGAGCCACCGCATGATGGCCTTATGAAAAGACCACCAGTTGGAAGGAATATTAAATTCATCACTGGGATCATGTGGAGGAATATTATCGGTCAGAGTATCTATCAAATTGCTGTCCTTCTGGTTCTTAATTTTTGTGGGAAACGTCTTTTAAAACTCACTGGTCCAAATGCAAGTTCCACTCTCAAGACCTTCATATTCAACTGTTTTGTATTTTGCCAGGTATTCAACGAGATTAACAGCCGTGATATGGAGAAAATCAATGTGTTCAGAGGGATGTTTAATAGTTATACTTTCATGATAGTGATGATCTGCACGGTAGTTTTCCAGATCATAATAGTTGAATTTTTGGGCACTTTTGCTCAAACTGTGCCACTGAGCTGGAAGTTGTGGTTAGCAAGCGTCTTGATTGGAGCCGTAAGTTTACCGGTTTCTGTTGTCTTGAAGTGCATTCCTGTATCATTAAGGAAACAAGCTGCTGAGGATCACAGTGATATCCACGAACCTCTTCTCCGTGGCCCTGAGCTGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009705 GO:0010035 GO:0010941 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055081 GO:0055085 GO:0060548 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098542 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase Hydrolase_3

Protein Analysis

1042

Amino Acids

114.2

Weight (kDa)

8.59

Isoelectric Point (pI)

38.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaATP_NAI PF12515 9 - 53 2.4e-20 Ca2+-ATPase N terminal autoinhibitory domain
Cation_ATPase_N PF00690 121 - 187 2.3e-10 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 240 - 334 2.3e-22 P-type ATPase actuator domain
Cation_ATPase PF13246 500 - 663 9.3e-33 P-type ATPase, cytoplasmic domain N
Hydrolase PF00702 648 - 770 1.6e-11 haloacid dehalogenase-like hydrolase
Cation_ATPase_C PF00689 841 - 1013 3.1e-41 Cation transporting ATPase, C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015798)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01980 FvH4_7g01980
malus_domestica MD02G1298100.v1.1 MD07G1026000.v1.1
prunus_persica Prupe.2G026200_v2.0.a1
pyrus_communis pycom07g01980
rosa_chinensis RchiOBHm_Chr1g0318981
rosa_laevigata RLG00000030512
rosa_multiflora Rmu_sc0003503.1_g000001 Rmu_sc0006626.1_g000004
rosa_roxburghii Rroxscaffold_4G00328710
rosa_rugosa Rorug01G0026100 Rorug01G0026200
rosa_samantha Rh1AG038000
rosa_wichuraiana Rw1G002930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1494
AarI CACCTGC 2 cut(s) 1033, 2173
AasI GACNNNNNNGTC 2 cut(s) 1168, 1784
Acc36I ACCTGC 2 cut(s) 1033, 2173
AccB7I CCANNNNNTGG 2 cut(s) 821, 2633
AccII CGCG 2 cut(s) 153, 331
AciI CCGC 5 cut(s) 118, 121, 127, 1350, 2603
AclWI GGATC 7 cut(s) 1427, 1970, 1983, 2190, 2524, 2669, 3084
AcoI YGGCCR 1 cut(s) 590
AcsI RAATTY 3 cut(s) 1601, 2648, 2936
AcuI CTGAAG 2 cut(s) 603, 1956
AfaI GTAC 3 cut(s) 1130, 1576, 2037
AfiI CCNNNNNNNGG 8 cut(s) 469, 485, 821, 2464, 2609, 2633, 2842, 3118
AgeI ACCGGT 1 cut(s) 3019
AhdI GACNNNNNGTC 2 cut(s) 92, 1269
AjnI CCWGG 3 cut(s) 183, 1714, 2811
AjuI GAANNNNNNNTTGG 4 cut(s) 43, 75, 2752, 2784
AleI CACNNNNGTG 1 cut(s) 2222
AloI GAACNNNNNNTCC 6 cut(s) 771, 795, 803, 827, 3087, 3119
Alw21I GWGCWC 1 cut(s) 1453
Alw26I GTCTC 8 cut(s) 79, 100, 562, 798, 1017, 1025, 1331, 2515
AlwI GGATC 7 cut(s) 1427, 1970, 1983, 2190, 2524, 2669, 3084
AlwNI CAGNNNCTG 2 cut(s) 1481, 2267
AoxI GGCC 9 cut(s) 69, 90, 454, 590, 823, 838, 2126, 2611, 3109
ApeKI GCWGC 3 cut(s) 221, 1097, 3068
ApoI RAATTY 3 cut(s) 1601, 2648, 2936
AseI ATTAAT 1 cut(s) 1952
AsiGI ACCGGT 1 cut(s) 3019
Asp700I GAANNNNTTC 1 cut(s) 3099
AspLEI GCGC 7 cut(s) 81, 153, 155, 174, 331, 1986, 2579
AspS9I GGNCC 4 cut(s) 1909, 2126, 2756, 3110
AsuII TTCGAA 1 cut(s) 39
AvaII GGWCC 2 cut(s) 1909, 2756
BaeGI GKGCMC 1 cut(s) 2948
BalI TGGCCA 1 cut(s) 592
BamHI GGATCC 1 cut(s) 1975
BanII GRGCYC 1 cut(s) 1349
BauI CACGAG 2 cut(s) 360, 2300
BbsI GAAGAC 1 cut(s) 2015
Bbv12I GWGCWC 1 cut(s) 1453
BbvCI CCTCAGC 1 cut(s) 3072
BbvI GCAGC 3 cut(s) 233, 1109, 3055
BceAI ACGGC 6 cut(s) 77, 1943, 1976, 1988, 2820, 2993
BcgI CGANNNNNNTGC 2 cut(s) 1722, 1756
BciT130I CCWGG 3 cut(s) 185, 1716, 2813
BciVI GTATCC 3 cut(s) 254, 409, 2560
BcoDI GTCTC 8 cut(s) 79, 100, 562, 798, 1017, 1025, 1331, 2515
BfaI CTAG 5 cut(s) 380, 732, 959, 1353, 1917
BfmI CTRYAG 7 cut(s) 667, 1040, 1212, 1593, 2118, 2180, 2592
BfoI RGCGCY 2 cut(s) 82, 2580
BfuAI ACCTGC 2 cut(s) 1033, 2173
BfuI GTATCC 3 cut(s) 254, 409, 2560
BisI GCNGC 5 cut(s) 118, 121, 222, 1098, 3069
BlsI GCNGC 5 cut(s) 119, 122, 223, 1099, 3070
Bme1390I CCNGG 3 cut(s) 185, 1716, 2813
Bme18I GGWCC 2 cut(s) 1909, 2756
BmeRI GACNNNNNGTC 2 cut(s) 92, 1269
BmgT120I GGNCC 4 cut(s) 1909, 2126, 2756, 3110
BmiI GGNNCC 7 cut(s) 928, 1348, 1381, 1911, 1977, 2127, 3007
BmrFI CCNGG 3 cut(s) 185, 1716, 2813
BmrI ACTGGG 1 cut(s) 2667
BmuI ACTGGG 1 cut(s) 2667
BpiI GAAGAC 1 cut(s) 2015
BplI GAGNNNNNCTC 4 cut(s) 189, 221, 305, 337
BpmI CTGGAG 2 cut(s) 103, 167
Bpu10I CCTNAGC 2 cut(s) 3072, 3114
Bpu14I TTCGAA 1 cut(s) 39
BpuEI CTTGAG 3 cut(s) 46, 94, 2762
Bsa29I ATCGAT 1 cut(s) 237
BsaBI GATNNNNATC 1 cut(s) 1418
BsaI GGTCTC 2 cut(s) 562, 798
BsaJI CCNNGG 3 cut(s) 156, 345, 3106
BsaWI WCCGGW 1 cut(s) 3019
BsaXI ACNNNNNCTCC 6 cut(s) 771, 795, 801, 825, 3087, 3117
Bsc4I CCNNNNNNNGG 8 cut(s) 469, 485, 821, 2464, 2609, 2633, 2842, 3118
Bse118I RCCGGY 1 cut(s) 3019
Bse3DI GCAATG 3 cut(s) 224, 573, 1290
Bse8I GATNNNNATC 1 cut(s) 1418
BseBI CCWGG 3 cut(s) 185, 1716, 2813
BseCI ATCGAT 1 cut(s) 237
BseDI CCNNGG 3 cut(s) 156, 345, 3106
BseGI GGATG 6 cut(s) 18, 501, 534, 2046, 2383, 2874
BseJI GATNNNNATC 1 cut(s) 1418
BseLI CCNNNNNNNGG 8 cut(s) 469, 485, 821, 2464, 2609, 2633, 2842, 3118
BseMI GCAATG 3 cut(s) 224, 573, 1290
BseMII CTCAG 9 cut(s) 1001, 1259, 1466, 1545, 1809, 2526, 2960, 3063, 3105
BsePI GCGCGC 1 cut(s) 151
BseRI GAGGAG 6 cut(s) 378, 381, 1035, 1658, 1835, 2514
BseSI GKGCMC 1 cut(s) 2948
BseXI GCAGC 3 cut(s) 233, 1109, 3055
BseYI CCCAGC 1 cut(s) 2024
BsgI GTGCAG 3 cut(s) 233, 1173, 2890
Bsh1236I CGCG 2 cut(s) 153, 331
BshFI GGCC 9 cut(s) 71, 92, 456, 592, 825, 840, 2128, 2613, 3111
BshTI ACCGGT 1 cut(s) 3019
BshVI ATCGAT 1 cut(s) 237
BsiHKAI GWGCWC 1 cut(s) 1453
BsiSI CCGG 2 cut(s) 2334, 3020
BslFI GGGAC 2 cut(s) 1005, 2290
BslI CCNNNNNNNGG 8 cut(s) 469, 485, 821, 2464, 2609, 2633, 2842, 3118
BsmAI GTCTC 8 cut(s) 79, 100, 562, 798, 1017, 1025, 1331, 2515
BsmBI CGTCTC 3 cut(s) 100, 1331, 2515
BsmFI GGGAC 2 cut(s) 1005, 2290
BsmI GAATGC 2 cut(s) 1736, 3042
BsnI GGCC 9 cut(s) 71, 92, 456, 592, 825, 840, 2128, 2613, 3111
Bso31I GGTCTC 2 cut(s) 562, 798
Bsp119I TTCGAA 1 cut(s) 39
Bsp1286I GDGCHC 3 cut(s) 1349, 1453, 2948
Bsp143I GATC 9 cut(s) 139, 1419, 1975, 2195, 2516, 2661, 2901, 2922, 3076
BspACI CCGC 5 cut(s) 118, 121, 127, 1350, 2603
BspANI GGCC 9 cut(s) 71, 92, 456, 592, 825, 840, 2128, 2613, 3111
BspCNI CTCAG 9 cut(s) 1002, 1260, 1465, 1544, 1810, 2525, 2961, 3064, 3106
BspDI ATCGAT 1 cut(s) 237
BspFNI CGCG 2 cut(s) 153, 331
BspHI TCATGA 1 cut(s) 2889
BspLI GGNNCC 7 cut(s) 928, 1348, 1381, 1911, 1977, 2127, 3007
BspMAI CTGCAG 3 cut(s) 1044, 1216, 2184
BspMI ACCTGC 2 cut(s) 1033, 2173
BspPI GGATC 7 cut(s) 1427, 1970, 1983, 2190, 2524, 2669, 3084
BspT104I TTCGAA 1 cut(s) 39
BspTNI GGTCTC 2 cut(s) 562, 798
BsrDI GCAATG 3 cut(s) 224, 573, 1290
BsrFI RCCGGY 1 cut(s) 3019
BssAI RCCGGY 1 cut(s) 3019
BssECI CCNNGG 3 cut(s) 156, 345, 3106
BssHII GCGCGC 1 cut(s) 151
BssMI GATC 9 cut(s) 139, 1419, 1975, 2195, 2516, 2661, 2901, 2922, 3076
BssSI CACGAG 2 cut(s) 360, 2300
BssT1I CCWWGG 1 cut(s) 345
Bst2BI CACGAG 2 cut(s) 360, 2300
Bst2UI CCWGG 3 cut(s) 185, 1716, 2813
Bst4CI ACNGT 8 cut(s) 266, 1567, 2035, 2467, 2798, 2911, 2962, 3083
Bst6I CTCTTC 1 cut(s) 3105
BstAPI GCANNNNNTGC 1 cut(s) 679
BstBI TTCGAA 1 cut(s) 39
BstC8I GCNNGC 7 cut(s) 153, 318, 884, 1610, 2506, 2992, 3120
BstDSI CCRYGG 1 cut(s) 3106
BstENI CCTNNNNNAGG 1 cut(s) 483
BstF5I GGATG 6 cut(s) 18, 501, 534, 2046, 2383, 2874
BstFNI CGCG 2 cut(s) 153, 331
BstH2I RGCGCY 2 cut(s) 82, 2580
BstHHI GCGC 7 cut(s) 81, 153, 155, 174, 331, 1986, 2579
BstKTI GATC 9 cut(s) 142, 1422, 1978, 2198, 2519, 2664, 2904, 2925, 3079
BstMAI GTCTC 8 cut(s) 79, 100, 562, 798, 1017, 1025, 1331, 2515
BstMBI GATC 9 cut(s) 139, 1419, 1975, 2195, 2516, 2661, 2901, 2922, 3076
BstMWI GCNNNNNNNGC 8 cut(s) 126, 143, 152, 326, 380, 679, 1097, 1868
BstNI CCWGG 3 cut(s) 185, 1716, 2813
BstNSI RCATGY 3 cut(s) 1335, 1612, 2508
BstSCI CCNGG 3 cut(s) 183, 1714, 2811
BstSFI CTRYAG 7 cut(s) 667, 1040, 1212, 1593, 2118, 2180, 2592
BstSLI GKGCMC 1 cut(s) 2948
BstUI CGCG 2 cut(s) 153, 331
BstV1I GCAGC 3 cut(s) 233, 1109, 3055
BstV2I GAAGAC 1 cut(s) 2015
BstX2I RGATCY 2 cut(s) 1419, 1975
BstXI CCANNNNNNTGG 2 cut(s) 352, 2973
BstYI RGATCY 2 cut(s) 1419, 1975
Bsu15I ATCGAT 1 cut(s) 237
BsuI GTATCC 3 cut(s) 254, 409, 2560
BsuRI GGCC 9 cut(s) 71, 92, 456, 592, 825, 840, 2128, 2613, 3111
BsuTUI ATCGAT 1 cut(s) 237
BtgI CCRYGG 1 cut(s) 3106
BtsCI GGATG 6 cut(s) 18, 501, 534, 2046, 2383, 2874
BtsI GCAGTG 4 cut(s) 651, 1037, 1266, 2529
BveI ACCTGC 2 cut(s) 1033, 2173
Cac8I GCNNGC 7 cut(s) 153, 318, 884, 1610, 2506, 2992, 3120
CaiI CAGNNNCTG 2 cut(s) 1481, 2267
CciI TCATGA 1 cut(s) 2889
CfoI GCGC 7 cut(s) 81, 153, 155, 174, 331, 1986, 2579
Cfr10I RCCGGY 1 cut(s) 3019
Cfr13I GGNCC 4 cut(s) 1909, 2126, 2756, 3110
ClaI ATCGAT 1 cut(s) 237
CseI GACGC 3 cut(s) 750, 2497, 2983
Csp6I GTAC 3 cut(s) 1129, 1575, 2036
CspAI ACCGGT 1 cut(s) 3019
CviAII CATG 9 cut(s) 130, 338, 1159, 1332, 1609, 2505, 2606, 2665, 2890
CviQI GTAC 3 cut(s) 1129, 1575, 2036
DpnI GATC 9 cut(s) 141, 1421, 1977, 2197, 2518, 2663, 2903, 2924, 3078
DpnII GATC 9 cut(s) 139, 1419, 1975, 2195, 2516, 2661, 2901, 2922, 3076
DraI TTTAAA 3 cut(s) 688, 1630, 2745
DrdI GACNNNNNNGTC 2 cut(s) 1168, 1784
DriI GACNNNNNGTC 2 cut(s) 92, 1269
DseDI GACNNNNNNGTC 2 cut(s) 1168, 1784
EaeI YGGCCR 1 cut(s) 590
Eam1104I CTCTTC 1 cut(s) 3105
Eam1105I GACNNNNNGTC 2 cut(s) 92, 1269
EarI CTCTTC 1 cut(s) 3105
Eco130I CCWWGG 1 cut(s) 345
Eco147I AGGCCT 1 cut(s) 71
Eco24I GRGCYC 1 cut(s) 1349
Eco31I GGTCTC 2 cut(s) 562, 798
Eco32I GATATC 1 cut(s) 3088
Eco47I GGWCC 2 cut(s) 1909, 2756
Eco57I CTGAAG 2 cut(s) 603, 1956
EcoNI CCTNNNNNAGG 1 cut(s) 483
EcoO109I RGGNCCY 1 cut(s) 1909
EcoRII CCWGG 3 cut(s) 183, 1714, 2811
EcoRV GATATC 1 cut(s) 3088
EcoT14I CCWWGG 1 cut(s) 345
EcoT22I ATGCAT 1 cut(s) 1186
EcoT38I GRGCYC 1 cut(s) 1349
ErhI CCWWGG 1 cut(s) 345
Esp3I CGTCTC 3 cut(s) 100, 1331, 2515
FaeI CATG 9 cut(s) 133, 341, 1162, 1335, 1612, 2508, 2609, 2668, 2893
FaqI GGGAC 2 cut(s) 1005, 2290
FatI CATG 9 cut(s) 129, 337, 1158, 1331, 1608, 2504, 2605, 2664, 2889
FauNDI CATATG 1 cut(s) 1398
Fnu4HI GCNGC 5 cut(s) 118, 121, 222, 1098, 3069
FokI GGATG 6 cut(s) 25, 508, 521, 2053, 2390, 2881
FriOI GRGCYC 1 cut(s) 1349
Fsp4HI GCNGC 5 cut(s) 118, 121, 222, 1098, 3069
FspBI CTAG 5 cut(s) 380, 732, 959, 1353, 1917
GlaI GCGC 7 cut(s) 80, 152, 154, 173, 330, 1985, 2578
GluI GCNGC 5 cut(s) 118, 121, 222, 1098, 3069
GsaI CCCAGC 1 cut(s) 2028
GsuI CTGGAG 2 cut(s) 103, 167
HaeII RGCGCY 2 cut(s) 82, 2580
HaeIII GGCC 9 cut(s) 71, 92, 456, 592, 825, 840, 2128, 2613, 3111
HapII CCGG 2 cut(s) 2334, 3020
HgaI GACGC 3 cut(s) 750, 2497, 2983
HhaI GCGC 7 cut(s) 81, 153, 155, 174, 331, 1986, 2579
Hin1II CATG 9 cut(s) 133, 341, 1162, 1335, 1612, 2508, 2609, 2668, 2893
Hin6I GCGC 7 cut(s) 79, 151, 153, 172, 329, 1984, 2577
HinP1I GCGC 7 cut(s) 79, 151, 153, 172, 329, 1984, 2577
HincII GTYRAC 2 cut(s) 1138, 2463
HindII GTYRAC 2 cut(s) 1138, 2463
HindIII AAGCTT 2 cut(s) 865, 1869
HinfI GANTC 4 cut(s) 721, 860, 1270, 2316
HpaI GTTAAC 2 cut(s) 1138, 2463
HpaII CCGG 2 cut(s) 2334, 3020
Hpy166II GTNNAC 5 cut(s) 892, 1138, 2463, 2554, 3017
Hpy8I GTNNAC 5 cut(s) 892, 1138, 2463, 2554, 3017
Hpy99I CGWCG 2 cut(s) 68, 104
HpyCH4III ACNGT 8 cut(s) 266, 1567, 2035, 2467, 2798, 2911, 2962, 3083
HpyCH4IV ACGT 1 cut(s) 2737
HpyF10VI GCNNNNNNNGC 8 cut(s) 126, 143, 152, 326, 380, 679, 1097, 1868
HpySE526I ACGT 1 cut(s) 2737
Hsp92II CATG 9 cut(s) 133, 341, 1162, 1335, 1612, 2508, 2609, 2668, 2893
HspAI GCGC 7 cut(s) 79, 151, 153, 172, 329, 1984, 2577
KspAI GTTAAC 2 cut(s) 1138, 2463
Kzo9I GATC 9 cut(s) 139, 1419, 1975, 2195, 2516, 2661, 2901, 2922, 3076
LmnI GCTCC 6 cut(s) 1352, 1748, 1822, 2293, 2527, 3005
Lsp1109I GCAGC 3 cut(s) 233, 1109, 3055
MaeI CTAG 5 cut(s) 380, 732, 959, 1353, 1917
MaeII ACGT 1 cut(s) 2737
MalI GATC 9 cut(s) 141, 1421, 1977, 2197, 2518, 2663, 2903, 2924, 3078
MboI GATC 9 cut(s) 139, 1419, 1975, 2195, 2516, 2661, 2901, 2922, 3076
MboII GAAGA 5 cut(s) 1562, 1949, 2020, 2117, 3092
MfeI CAATTG 1 cut(s) 798
MflI RGATCY 2 cut(s) 1419, 1975
MhlI GDGCHC 3 cut(s) 1349, 1453, 2948
MlsI TGGCCA 1 cut(s) 592
MluNI TGGCCA 1 cut(s) 592
MlyI GAGTC 2 cut(s) 1279, 2310
MmeI TCCRAC 4 cut(s) 87, 757, 1604, 2614
Mox20I TGGCCA 1 cut(s) 592
Mph1103I ATGCAT 1 cut(s) 1186
MroXI GAANNNNTTC 1 cut(s) 3099
MscI TGGCCA 1 cut(s) 592
MslI CAYNNNNRTG 3 cut(s) 539, 2222, 2894
Msp20I TGGCCA 1 cut(s) 592
MspA1I CMGCKG 2 cut(s) 818, 1100
MspI CCGG 2 cut(s) 2334, 3020
MspR9I CCNGG 3 cut(s) 185, 1716, 2813
MunI CAATTG 1 cut(s) 798
Mva1269I GAATGC 2 cut(s) 1736, 3042
MvaI CCWGG 3 cut(s) 185, 1716, 2813
MvnI CGCG 2 cut(s) 153, 331
MwoI GCNNNNNNNGC 8 cut(s) 126, 143, 152, 326, 380, 679, 1097, 1868
NdeI CATATG 1 cut(s) 1398
NdeII GATC 9 cut(s) 139, 1419, 1975, 2195, 2516, 2661, 2901, 2922, 3076
NlaIII CATG 9 cut(s) 133, 341, 1162, 1335, 1612, 2508, 2609, 2668, 2893
NlaIV GGNNCC 7 cut(s) 928, 1348, 1381, 1911, 1977, 2127, 3007
NmeAIII GCCGAG 1 cut(s) 181
NmuCI GTSAC 9 cut(s) 649, 662, 1102, 1261, 1301, 1424, 1775, 2044, 2263
NsiI ATGCAT 1 cut(s) 1186
NspI RCATGY 3 cut(s) 1335, 1612, 2508
NspV TTCGAA 1 cut(s) 39
OliI CACNNNNGTG 1 cut(s) 2222
PaeI GCATGC 2 cut(s) 1612, 2508
PagI TCATGA 1 cut(s) 2889
PaqCI CACCTGC 2 cut(s) 1033, 2173
PauI GCGCGC 1 cut(s) 151
PceI AGGCCT 1 cut(s) 71
PctI GAATGC 2 cut(s) 1736, 3042
PdmI GAANNNNTTC 1 cut(s) 3099
PfeI GAWTC 2 cut(s) 721, 860
PflMI CCANNNNNTGG 2 cut(s) 821, 2633
PfoI TCCNGGA 1 cut(s) 183
PinAI ACCGGT 1 cut(s) 3019
PkrI GCNGC 5 cut(s) 119, 122, 223, 1099, 3070
PleI GAGTC 2 cut(s) 1278, 2310
PpsI GAGTC 2 cut(s) 1278, 2310
PpuMI RGGWCCY 1 cut(s) 1909
PshBI ATTAAT 1 cut(s) 1952
PsiI TTATAA 1 cut(s) 1494
Psp5II RGGWCCY 1 cut(s) 1909
Psp6I CCWGG 3 cut(s) 183, 1714, 2811
PspFI CCCAGC 1 cut(s) 2024
PspGI CCWGG 3 cut(s) 183, 1714, 2811
PspN4I GGNNCC 7 cut(s) 928, 1348, 1381, 1911, 1977, 2127, 3007
PspPI GGNCC 4 cut(s) 1909, 2126, 2756, 3110
PspPPI RGGWCCY 1 cut(s) 1909
PsrI GAACNNNNNNTAC 2 cut(s) 325, 357
PstI CTGCAG 3 cut(s) 1044, 1216, 2184
PstNI CAGNNNCTG 2 cut(s) 1481, 2267
PsuI RGATCY 2 cut(s) 1419, 1975
PteI GCGCGC 1 cut(s) 151
PvuII CAGCTG 2 cut(s) 818, 1100
RsaI GTAC 3 cut(s) 1130, 1576, 2037
RsaNI GTAC 3 cut(s) 1129, 1575, 2036
RseI CAYNNNNRTG 3 cut(s) 539, 2222, 2894
SatI GCNGC 5 cut(s) 118, 121, 222, 1098, 3069
Sau3AI GATC 9 cut(s) 139, 1419, 1975, 2195, 2516, 2661, 2901, 2922, 3076
Sau96I GGNCC 4 cut(s) 1909, 2126, 2756, 3110
SchI GAGTC 2 cut(s) 1279, 2310
ScrFI CCNGG 3 cut(s) 185, 1716, 2813
SduI GDGCHC 3 cut(s) 1349, 1453, 2948
SfcI CTRYAG 7 cut(s) 667, 1040, 1212, 1593, 2118, 2180, 2592
SfuI TTCGAA 1 cut(s) 39
SinI GGWCC 2 cut(s) 1909, 2756
SmiMI CAYNNNNRTG 3 cut(s) 539, 2222, 2894
SmlI CTYRAG 3 cut(s) 25, 73, 2777
SmoI CTYRAG 3 cut(s) 25, 73, 2777
SphI GCATGC 2 cut(s) 1612, 2508
SseBI AGGCCT 1 cut(s) 71
SsiI CCGC 5 cut(s) 118, 121, 127, 1350, 2603
SspI AATATT 4 cut(s) 624, 2473, 2644, 2677
SspMI CTAG 5 cut(s) 380, 732, 959, 1353, 1917
StuI AGGCCT 1 cut(s) 71
StyD4I CCNGG 3 cut(s) 183, 1714, 2811
StyI CCWWGG 1 cut(s) 345
TaaI ACNGT 8 cut(s) 266, 1567, 2035, 2467, 2798, 2911, 2962, 3083
TaiI ACGT 1 cut(s) 2740
TaqI TCGA 5 cut(s) 39, 45, 237, 1665, 1732
TaqII GACCGA 1 cut(s) 2672
TauI GCSGC 2 cut(s) 120, 123
TfiI GAWTC 2 cut(s) 721, 860
TseFI GTSAC 9 cut(s) 649, 662, 1102, 1261, 1301, 1424, 1775, 2044, 2263
TseI GCWGC 3 cut(s) 221, 1097, 3068
Tsp45I GTSAC 9 cut(s) 649, 662, 1102, 1261, 1301, 1424, 1775, 2044, 2263
TspDTI ATGAA 9 cut(s) 223, 779, 873, 1190, 1301, 2633, 2641, 2776, 2878
TspGWI ACGGA 3 cut(s) 88, 2053, 3095
Van91I CCANNNNNTGG 2 cut(s) 821, 2633
VpaK11BI GGWCC 2 cut(s) 1909, 2756
VspI ATTAAT 1 cut(s) 1952
XagI CCTNNNNNAGG 1 cut(s) 483
XapI RAATTY 3 cut(s) 1601, 2648, 2936
XceI RCATGY 3 cut(s) 1335, 1612, 2508
XmnI GAANNNNTTC 1 cut(s) 3099
XspI CTAG 5 cut(s) 380, 732, 959, 1353, 1917
Zsp2I ATGCAT 1 cut(s) 1186
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.