Rh1AG038000

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0015798
Physical Location & Seq
Forward (+)
6650330 .. 6658019
7690 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG038000.1

Sequence Viewer

Length: 2961 bp
ATGCTCCTATTGATTGTCCAGGAAAAGATACGAGTAGCTCTCTATGTGCAGAAAGCAGCATTGCAATTCATCGATGCTGGTAATAAAGAAGCTGGATACAGTCTTTCCAAAGAAGTTAGTGATGCTGGTTTTGGCATTTCACCTGATGAGCTTGCATCCTTTGCGCGAACCCATGATACCAAGGGTGTGGAAGCACGAGGAGGAGTAGCAGGACTAGCAGGAGATATATCAGTATCCTTGAAAGAAGGCGTTGTTTCAAGCAAGATACCTGTTAGGCAAAACATATATGGCCTCAACCGATTTGTGGAGAAGCCTTCTAAAGGTTTTTGGATGTTTGTCTGGGAAGCCATACAAGATTTAACTCTAATCATCCTTATGGTTTCTGCTGTGGTCTCTATTGGCGTTGGCATTGCAACTGAAGGGTGGCCAAAGGGTATGTATGATGGGTTGGGTATAATATTGAGCATCTTATTGGTTGTGATGGTCACTGCAATCAGTGACTACAGGCAATCTTTGCAATTTAAAGAACTGGATAAGGAGAAAAAAAATATAAAGATTCAGGTAACTAGAGATGGACGCAGACAGAAGGTTTCTATCTACGATTTGGTGGTTGGAGATATTGTTCATCTTTCAATTGGAGACCAAGTTCCAGCTGATGGCCTTCTCATATCAGGCCATAGCTTACAAATTGATGAATCAAGCTTGTCAGGTGAGAGCGAGCCAGTGGACATAAATCAAAAGAGACCTTTTATTCTTGGAGGAACCAAAGTGCAAGATGGCTCTGGTAAAATGCTAGTTACATCTGTTGGTATGAGAACTGAATGGGGACGACTAATGGTTACTCTGAGTGAAGGAGGAGACAATGAGACACCACTGCAGGTGAAACTCAATGGTGTTGCTACCATTATTGGAAAAATTGGTTTGGCTTTTGCAGCTGTCACATTTGTAGTTCTGACTACGAGGTACTTAGTTAACAAGGGAACGCACCATAACATGACTAACTGGTCTATGAAAGATGCATCAGCACTTTTGAGTTACTTTTCTACTGCAGTTATCATAATAGTTGTTGCTGTTCCAGAGGGGCTTCCTCTGGCAGTGACACTGAGTCTTGCCTTTGCAATGAAAAAGCTAATGGGTGACAAGGCACTTGTGAGGCATCTTTCAGCATGTGAGACGATGGGCTCTGCTAGTTGTATTTGCACAGATAAGACGGGAACCTTGACAACAAATCATATGGTGGTGAATAAGATATGGATATGTGACGAAGCCAAGACTATAACGAGTGGTCAGAACAAAGATGTATTGAAGTCAGTTTCTGAAAAAGTTTATAACCTTCTTTTGCAGTCCATCTTTCTGAATACAACCTCAGAGGTAGTAAAAGGGGAAGATGGGAAAAATACTGTTATAGGTACTCCAACAGAGATGGCTATAGTAGAATTTGGCATGCTTCTGGGAGGTGATTTTAAATCTTTTAGTGAGGAGTATAAGATAGTTATGGTCGAACCATTCAATTCAATCAGAAAAAAGATGTCGGTTCAAGTGGCTCTTCCTGGTAGTGATATGTGTCGAGCATTCTGCAAAGGAGCATCAGAAATTATTTTAGGAATGTGTGACAAGGTTGTCAATACTGATGGTGAAGCTATGCCTTTGTCTGAGGAGCAGAGAAAGAAAATATCTGATGTCATAAATGGTTTTGCTTGTGAAGCTTTAAGAACTCTATGCGTAGCTTTCAAGGATATAGAGGACCCTTCTAGTGCAAAAAGTATTCCTGAAGATAAATATACATTAATAGCTGTTGTGGGAATTAAGGATCCTGTGCGCCCTGGCGTGAGGGAAGCCGTGAAGACTTGTTTAGATGCTGGGATTACTGTACGGATGGTCACCGGTGATAATATCAATACAGCTAAAGCCATAGCTAAAGAGTGTGGCATTTTGACAGAAGATGGTTTAGCTATAGAAGGGCCAGATTTCCAAAAAATGAGCCAACAGGAGATGGCAGAAATAATCCCAAAACTGCAGGTAATGGCACGATCCTTGCCTTTGGACAAGCACACTTTAGTGAAGCAGTTGAGGAATGTGCATAAAGAAGTAGTGGCAGTAACTGGTGATGGGACTAATGATGCTCCTGCTTTGCACGAGGCAGACATTGGACTCGCTATGGGTATAGCCGGAACAGAGGTTGCAAAAGAAAATGCTGATGTTATCATTATGGATGACAACTTTACAAGTATAGTGAATGTTGCAAGATGGGGTCGTTCAGTTTATATCAACATTCAAAAGTTTGTTCAGTTCCAGTTAACGGTTAATATTGTCGCTCTGATGCTCAATTTCGTATCAGCATGCGTCTCAGGATCAGCTCCTCTCACTGCTGTCCAGATGCTTTGGGTAAACTTGATTATGGATACTCTTGGCGCTTTGGCACTGGCTACAGAGCCACCGCATGATGGCCTTATGAAAAGACCACCAGTTGGAAGGAATATTAAATTCATCACTGGGATCATGTGGAGGAATATTATCGGTCAGAGTATCTATCAAATTGCTGTCCTTCTGGTTCTTAATTTTTGTGGGAAACGTCTTTTAAAACTCACCAGTCCAAATGCAAGTTCCATTCTCAAGACCTTCATATTCAACTGTTTTGTATTTTGCCAGGTATTCAACGAGATTAACAGCCGTGATATGGAGAAAATCAATGTGTTCAGAGGGATGTTTAATAGTTATACTTTCATGATAGTGATGATCTGCACAGTAGTTTTCCAGATCATAATAGTTGAATTTTTGGGCACTTTTGCTCAAACTGTGCCACTGAGCTGGAAGTTGTGGTTAGCAAGCGTCTTGATTGGAGCCGTAAGTTTACCAGTTGCTGTTGTCTTGAAGTGCATTCCTGTATCATTAAGGAAACAAGCTGCTAAGGATCACAGTGATATCCACGAACCTCTTCTCCGTGGCCCTGAGCTGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009705 GO:0010035 GO:0010941 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055081 GO:0055085 GO:0060548 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098542 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase Hydrolase_3

Protein Analysis

986

Amino Acids

107.26

Weight (kDa)

6.9

Isoelectric Point (pI)

35.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cation_ATPase_N PF00690 66 - 132 2.1e-10 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 185 - 279 1e-22 P-type ATPase actuator domain
Cation_ATPase PF13246 443 - 608 2.9e-33 P-type ATPase, cytoplasmic domain N
Hydrolase PF00702 593 - 715 7e-13 haloacid dehalogenase-like hydrolase
Cation_ATPase_C PF00689 786 - 959 5.6e-41 Cation transporting ATPase, C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015798)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01980 FvH4_7g01980
malus_domestica MD02G1298100.v1.1 MD07G1026000.v1.1
prunus_persica Prupe.2G026200_v2.0.a1
pyrus_communis pycom07g01980
rosa_chinensis RchiOBHm_Chr1g0318981
rosa_laevigata RLG00000030512
rosa_multiflora Rmu_sc0003503.1_g000001 Rmu_sc0006626.1_g000004
rosa_roxburghii Rroxscaffold_4G00328710
rosa_rugosa Rorug01G0026100 Rorug01G0026200
rosa_samantha Rh1AG038000
rosa_wichuraiana Rw1G002930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1329
AarI CACCTGC 1 cut(s) 868
AasI GACNNNNNNGTC 2 cut(s) 1003, 1619
Acc36I ACCTGC 2 cut(s) 868, 2008
AccB7I CCANNNNNTGG 2 cut(s) 656, 2468
AccII CGCG 1 cut(s) 166
AciI CCGC 1 cut(s) 2438
AclWI GGATC 6 cut(s) 1805, 1818, 2025, 2359, 2504, 2919
AcoI YGGCCR 1 cut(s) 425
AcsI RAATTY 3 cut(s) 1436, 2483, 2771
AcuI CTGAAG 2 cut(s) 438, 1791
AfaI GTAC 3 cut(s) 965, 1411, 1872
AfiI CCNNNNNNNGG 8 cut(s) 304, 320, 656, 2299, 2444, 2468, 2677, 2953
AgeI ACCGGT 1 cut(s) 1883
AhdI GACNNNNNGTC 1 cut(s) 1104
AjnI CCWGG 4 cut(s) 18, 1549, 1822, 2646
AjuI GAANNNNNNNTTGG 2 cut(s) 2587, 2619
AleI CACNNNNGTG 1 cut(s) 2057
AloI GAACNNNNNNTCC 6 cut(s) 606, 630, 638, 662, 2922, 2954
Alw26I GTCTC 7 cut(s) 397, 633, 736, 852, 860, 1166, 2350
AlwI GGATC 6 cut(s) 1805, 1818, 2025, 2359, 2504, 2919
AlwNI CAGNNNCTG 3 cut(s) 1316, 2102, 2861
AoxI GGCC 7 cut(s) 289, 425, 658, 673, 1961, 2446, 2944
ApeKI GCWGC 3 cut(s) 56, 932, 2903
ApoI RAATTY 3 cut(s) 1436, 2483, 2771
AseI ATTAAT 1 cut(s) 1787
AsiGI ACCGGT 1 cut(s) 1883
Asp700I GAANNNNTTC 1 cut(s) 2934
AspLEI GCGC 3 cut(s) 166, 1821, 2414
AspS9I GGNCC 3 cut(s) 1744, 1961, 2945
AvaII GGWCC 1 cut(s) 1744
BaeGI GKGCMC 1 cut(s) 2783
BalI TGGCCA 1 cut(s) 427
BamHI GGATCC 1 cut(s) 1810
BanII GRGCYC 1 cut(s) 1184
BauI CACGAG 2 cut(s) 195, 2135
BbsI GAAGAC 1 cut(s) 1850
BbvI GCAGC 3 cut(s) 68, 944, 2890
BceAI ACGGC 3 cut(s) 1823, 2655, 2828
BcgI CGANNNNNNTGC 2 cut(s) 1557, 1591
BciT130I CCWGG 4 cut(s) 20, 1551, 1824, 2648
BciVI GTATCC 3 cut(s) 89, 244, 2395
BcoDI GTCTC 7 cut(s) 397, 633, 736, 852, 860, 1166, 2350
BfaI CTAG 5 cut(s) 215, 567, 794, 1188, 1752
BfmI CTRYAG 7 cut(s) 502, 875, 1047, 1428, 1953, 2015, 2427
BfoI RGCGCY 1 cut(s) 2415
BfuAI ACCTGC 2 cut(s) 868, 2008
BfuI GTATCC 3 cut(s) 89, 244, 2395
BisI GCNGC 3 cut(s) 57, 933, 2904
BlsI GCNGC 3 cut(s) 58, 934, 2905
Bme1390I CCNGG 4 cut(s) 20, 1551, 1824, 2648
Bme18I GGWCC 1 cut(s) 1744
BmeRI GACNNNNNGTC 1 cut(s) 1104
BmgT120I GGNCC 3 cut(s) 1744, 1961, 2945
BmiI GGNNCC 5 cut(s) 763, 1216, 1746, 1812, 2842
BmrFI CCNGG 4 cut(s) 20, 1551, 1824, 2648
BmrI ACTGGG 1 cut(s) 2502
BmuI ACTGGG 1 cut(s) 2502
BpiI GAAGAC 1 cut(s) 1850
BplI GAGNNNNNCTC 2 cut(s) 24, 56
Bpu10I CCTNAGC 2 cut(s) 2907, 2949
BpuEI CTTGAG 1 cut(s) 2597
Bsa29I ATCGAT 1 cut(s) 72
BsaI GGTCTC 3 cut(s) 397, 633, 736
BsaJI CCNNGG 3 cut(s) 180, 1822, 2941
BsaWI WCCGGW 1 cut(s) 1883
BsaXI ACNNNNNCTCC 6 cut(s) 606, 630, 636, 660, 2922, 2952
Bsc4I CCNNNNNNNGG 8 cut(s) 304, 320, 656, 2299, 2444, 2468, 2677, 2953
Bse118I RCCGGY 1 cut(s) 1883
Bse3DI GCAATG 3 cut(s) 59, 408, 1125
BseBI CCWGG 4 cut(s) 20, 1551, 1824, 2648
BseCI ATCGAT 1 cut(s) 72
BseDI CCNNGG 3 cut(s) 180, 1822, 2941
BseGI GGATG 6 cut(s) 155, 336, 369, 1881, 2218, 2709
BseLI CCNNNNNNNGG 8 cut(s) 304, 320, 656, 2299, 2444, 2468, 2677, 2953
BseMI GCAATG 3 cut(s) 59, 408, 1125
BseMII CTCAG 7 cut(s) 836, 1094, 1380, 1644, 2361, 2795, 2940
BseRI GAGGAG 6 cut(s) 213, 216, 870, 1493, 1670, 2349
BseSI GKGCMC 1 cut(s) 2783
BseXI GCAGC 3 cut(s) 68, 944, 2890
BseYI CCCAGC 1 cut(s) 1859
BsgI GTGCAG 2 cut(s) 68, 2725
Bsh1236I CGCG 1 cut(s) 166
BshFI GGCC 7 cut(s) 291, 427, 660, 675, 1963, 2448, 2946
BshTI ACCGGT 1 cut(s) 1883
BshVI ATCGAT 1 cut(s) 72
BsiSI CCGG 2 cut(s) 1884, 2169
BslFI GGGAC 2 cut(s) 840, 2125
BslI CCNNNNNNNGG 8 cut(s) 304, 320, 656, 2299, 2444, 2468, 2677, 2953
BsmAI GTCTC 7 cut(s) 397, 633, 736, 852, 860, 1166, 2350
BsmBI CGTCTC 2 cut(s) 1166, 2350
BsmFI GGGAC 2 cut(s) 840, 2125
BsmI GAATGC 2 cut(s) 1571, 2877
BsnI GGCC 7 cut(s) 291, 427, 660, 675, 1963, 2448, 2946
Bso31I GGTCTC 3 cut(s) 397, 633, 736
Bsp1286I GDGCHC 2 cut(s) 1184, 2783
Bsp143I GATC 7 cut(s) 1810, 2030, 2351, 2496, 2736, 2757, 2911
BspACI CCGC 1 cut(s) 2438
BspANI GGCC 7 cut(s) 291, 427, 660, 675, 1963, 2448, 2946
BspCNI CTCAG 7 cut(s) 837, 1095, 1379, 1645, 2360, 2796, 2941
BspDI ATCGAT 1 cut(s) 72
BspFNI CGCG 1 cut(s) 166
BspHI TCATGA 1 cut(s) 2724
BspLI GGNNCC 5 cut(s) 763, 1216, 1746, 1812, 2842
BspMAI CTGCAG 3 cut(s) 879, 1051, 2019
BspMI ACCTGC 2 cut(s) 868, 2008
BspPI GGATC 6 cut(s) 1805, 1818, 2025, 2359, 2504, 2919
BspQI GCTCTTC 1 cut(s) 1551
BspTNI GGTCTC 3 cut(s) 397, 633, 736
BsrDI GCAATG 3 cut(s) 59, 408, 1125
BsrFI RCCGGY 1 cut(s) 1883
BssAI RCCGGY 1 cut(s) 1883
BssECI CCNNGG 3 cut(s) 180, 1822, 2941
BssMI GATC 7 cut(s) 1810, 2030, 2351, 2496, 2736, 2757, 2911
BssSI CACGAG 2 cut(s) 195, 2135
BssT1I CCWWGG 1 cut(s) 180
Bst2BI CACGAG 2 cut(s) 195, 2135
Bst2UI CCWGG 4 cut(s) 20, 1551, 1824, 2648
Bst4CI ACNGT 8 cut(s) 101, 1402, 1870, 2302, 2633, 2746, 2797, 2918
Bst6I CTCTTC 2 cut(s) 1551, 2940
BstAPI GCANNNNNTGC 2 cut(s) 161, 514
BstC8I GCNNGC 6 cut(s) 153, 719, 1445, 2341, 2827, 2955
BstDEI CTNAG 9 cut(s) 845, 967, 1103, 1366, 1653, 2347, 2804, 2907, 2949
BstDSI CCRYGG 1 cut(s) 2941
BstEII GGTNACC 1 cut(s) 1879
BstENI CCTNNNNNAGG 1 cut(s) 318
BstF5I GGATG 6 cut(s) 155, 336, 369, 1881, 2218, 2709
BstFNI CGCG 1 cut(s) 166
BstH2I RGCGCY 1 cut(s) 2415
BstHHI GCGC 3 cut(s) 166, 1821, 2414
BstKTI GATC 7 cut(s) 1813, 2033, 2354, 2499, 2739, 2760, 2914
BstMAI GTCTC 7 cut(s) 397, 633, 736, 852, 860, 1166, 2350
BstMBI GATC 7 cut(s) 1810, 2030, 2351, 2496, 2736, 2757, 2911
BstMWI GCNNNNNNNGC 5 cut(s) 161, 215, 514, 932, 1703
BstNI CCWGG 4 cut(s) 20, 1551, 1824, 2648
BstNSI RCATGY 3 cut(s) 1170, 1447, 2343
BstPI GGTNACC 1 cut(s) 1879
BstSCI CCNGG 4 cut(s) 18, 1549, 1822, 2646
BstSFI CTRYAG 7 cut(s) 502, 875, 1047, 1428, 1953, 2015, 2427
BstSLI GKGCMC 1 cut(s) 2783
BstUI CGCG 1 cut(s) 166
BstV1I GCAGC 3 cut(s) 68, 944, 2890
BstV2I GAAGAC 1 cut(s) 1850
BstX2I RGATCY 1 cut(s) 1810
BstXI CCANNNNNNTGG 2 cut(s) 187, 2808
BstYI RGATCY 1 cut(s) 1810
Bsu15I ATCGAT 1 cut(s) 72
BsuI GTATCC 3 cut(s) 89, 244, 2395
BsuRI GGCC 7 cut(s) 291, 427, 660, 675, 1963, 2448, 2946
BsuTUI ATCGAT 1 cut(s) 72
BtgI CCRYGG 1 cut(s) 2941
BtsCI GGATG 6 cut(s) 155, 336, 369, 1881, 2218, 2709
BtsI GCAGTG 4 cut(s) 486, 872, 1101, 2364
BveI ACCTGC 2 cut(s) 868, 2008
Cac8I GCNNGC 6 cut(s) 153, 719, 1445, 2341, 2827, 2955
CaiI CAGNNNCTG 3 cut(s) 1316, 2102, 2861
CciI TCATGA 1 cut(s) 2724
CfoI GCGC 3 cut(s) 166, 1821, 2414
Cfr10I RCCGGY 1 cut(s) 1883
Cfr13I GGNCC 3 cut(s) 1744, 1961, 2945
ClaI ATCGAT 1 cut(s) 72
CseI GACGC 3 cut(s) 585, 2332, 2818
Csp6I GTAC 3 cut(s) 964, 1410, 1871
CspAI ACCGGT 1 cut(s) 1883
CviAII CATG 8 cut(s) 173, 994, 1167, 1444, 2340, 2441, 2500, 2725
CviQI GTAC 3 cut(s) 964, 1410, 1871
DdeI CTNAG 9 cut(s) 845, 967, 1103, 1366, 1653, 2347, 2804, 2907, 2949
DpnI GATC 7 cut(s) 1812, 2032, 2353, 2498, 2738, 2759, 2913
DpnII GATC 7 cut(s) 1810, 2030, 2351, 2496, 2736, 2757, 2911
DraI TTTAAA 3 cut(s) 523, 1465, 2580
DrdI GACNNNNNNGTC 2 cut(s) 1003, 1619
DriI GACNNNNNGTC 1 cut(s) 1104
DseDI GACNNNNNNGTC 2 cut(s) 1003, 1619
EaeI YGGCCR 1 cut(s) 425
Eam1104I CTCTTC 2 cut(s) 1551, 2940
Eam1105I GACNNNNNGTC 1 cut(s) 1104
EarI CTCTTC 2 cut(s) 1551, 2940
Eco130I CCWWGG 1 cut(s) 180
Eco24I GRGCYC 1 cut(s) 1184
Eco31I GGTCTC 3 cut(s) 397, 633, 736
Eco32I GATATC 1 cut(s) 2923
Eco47I GGWCC 1 cut(s) 1744
Eco57I CTGAAG 2 cut(s) 438, 1791
Eco91I GGTNACC 1 cut(s) 1879
EcoNI CCTNNNNNAGG 1 cut(s) 318
EcoO109I RGGNCCY 1 cut(s) 1744
EcoO65I GGTNACC 1 cut(s) 1879
EcoRII CCWGG 4 cut(s) 18, 1549, 1822, 2646
EcoRV GATATC 1 cut(s) 2923
EcoT14I CCWWGG 1 cut(s) 180
EcoT22I ATGCAT 1 cut(s) 1021
EcoT38I GRGCYC 1 cut(s) 1184
ErhI CCWWGG 1 cut(s) 180
Esp3I CGTCTC 2 cut(s) 1166, 2350
FaeI CATG 8 cut(s) 176, 997, 1170, 1447, 2343, 2444, 2503, 2728
FalI AAGNNNNNCTT 2 cut(s) 1530, 1562
FaqI GGGAC 2 cut(s) 840, 2125
FatI CATG 8 cut(s) 172, 993, 1166, 1443, 2339, 2440, 2499, 2724
FauNDI CATATG 1 cut(s) 1233
Fnu4HI GCNGC 3 cut(s) 57, 933, 2904
FokI GGATG 6 cut(s) 142, 343, 356, 1888, 2225, 2716
FriOI GRGCYC 1 cut(s) 1184
Fsp4HI GCNGC 3 cut(s) 57, 933, 2904
FspBI CTAG 5 cut(s) 215, 567, 794, 1188, 1752
GlaI GCGC 3 cut(s) 165, 1820, 2413
GluI GCNGC 3 cut(s) 57, 933, 2904
GsaI CCCAGC 1 cut(s) 1863
HaeII RGCGCY 1 cut(s) 2415
HaeIII GGCC 7 cut(s) 291, 427, 660, 675, 1963, 2448, 2946
HapII CCGG 2 cut(s) 1884, 2169
HgaI GACGC 3 cut(s) 585, 2332, 2818
HhaI GCGC 3 cut(s) 166, 1821, 2414
Hin1II CATG 8 cut(s) 176, 997, 1170, 1447, 2343, 2444, 2503, 2728
Hin6I GCGC 3 cut(s) 164, 1819, 2412
HinP1I GCGC 3 cut(s) 164, 1819, 2412
HincII GTYRAC 2 cut(s) 973, 2298
HindII GTYRAC 2 cut(s) 973, 2298
HindIII AAGCTT 2 cut(s) 700, 1704
HinfI GANTC 4 cut(s) 556, 695, 1105, 2151
HpaI GTTAAC 2 cut(s) 973, 2298
HpaII CCGG 2 cut(s) 1884, 2169
Hpy166II GTNNAC 5 cut(s) 727, 973, 2298, 2389, 2852
Hpy188III TCNNGA 9 cut(s) 1076, 1769, 2349, 2374, 2614, 2725, 2755, 2833, 2869
Hpy8I GTNNAC 5 cut(s) 727, 973, 2298, 2389, 2852
HpyCH4III ACNGT 8 cut(s) 101, 1402, 1870, 2302, 2633, 2746, 2797, 2918
HpyCH4IV ACGT 1 cut(s) 2572
HpyF10VI GCNNNNNNNGC 5 cut(s) 161, 215, 514, 932, 1703
HpyF3I CTNAG 9 cut(s) 845, 967, 1103, 1366, 1653, 2347, 2804, 2907, 2949
HpySE526I ACGT 1 cut(s) 2572
Hsp92II CATG 8 cut(s) 176, 997, 1170, 1447, 2343, 2444, 2503, 2728
HspAI GCGC 3 cut(s) 164, 1819, 2412
KspAI GTTAAC 2 cut(s) 973, 2298
Kzo9I GATC 7 cut(s) 1810, 2030, 2351, 2496, 2736, 2757, 2911
LguI GCTCTTC 1 cut(s) 1551
LmnI GCTCC 6 cut(s) 9, 1583, 1657, 2128, 2362, 2840
Lsp1109I GCAGC 3 cut(s) 68, 944, 2890
MaeI CTAG 5 cut(s) 215, 567, 794, 1188, 1752
MaeII ACGT 1 cut(s) 2572
MalI GATC 7 cut(s) 1812, 2032, 2353, 2498, 2738, 2759, 2913
MboI GATC 7 cut(s) 1810, 2030, 2351, 2496, 2736, 2757, 2911
MboII GAAGA 6 cut(s) 1397, 1538, 1784, 1855, 1952, 2927
MfeI CAATTG 1 cut(s) 633
MflI RGATCY 1 cut(s) 1810
MhlI GDGCHC 2 cut(s) 1184, 2783
MlsI TGGCCA 1 cut(s) 427
MluNI TGGCCA 1 cut(s) 427
MlyI GAGTC 2 cut(s) 1114, 2145
MmeI TCCRAC 3 cut(s) 592, 1439, 2449
Mox20I TGGCCA 1 cut(s) 427
Mph1103I ATGCAT 1 cut(s) 1021
MroXI GAANNNNTTC 1 cut(s) 2934
MscI TGGCCA 1 cut(s) 427
MslI CAYNNNNRTG 3 cut(s) 374, 2057, 2729
Msp20I TGGCCA 1 cut(s) 427
MspA1I CMGCKG 2 cut(s) 653, 935
MspI CCGG 2 cut(s) 1884, 2169
MspR9I CCNGG 4 cut(s) 20, 1551, 1824, 2648
MunI CAATTG 1 cut(s) 633
Mva1269I GAATGC 2 cut(s) 1571, 2877
MvaI CCWGG 4 cut(s) 20, 1551, 1824, 2648
MvnI CGCG 1 cut(s) 166
MwoI GCNNNNNNNGC 5 cut(s) 161, 215, 514, 932, 1703
NdeI CATATG 1 cut(s) 1233
NdeII GATC 7 cut(s) 1810, 2030, 2351, 2496, 2736, 2757, 2911
NlaIII CATG 8 cut(s) 176, 997, 1170, 1447, 2343, 2444, 2503, 2728
NlaIV GGNNCC 5 cut(s) 763, 1216, 1746, 1812, 2842
NmuCI GTSAC 8 cut(s) 484, 497, 937, 1096, 1136, 1259, 1610, 1879
NsiI ATGCAT 1 cut(s) 1021
NspI RCATGY 3 cut(s) 1170, 1447, 2343
OliI CACNNNNGTG 1 cut(s) 2057
PaeI GCATGC 2 cut(s) 1447, 2343
PagI TCATGA 1 cut(s) 2724
PaqCI CACCTGC 1 cut(s) 868
PciSI GCTCTTC 1 cut(s) 1551
PctI GAATGC 2 cut(s) 1571, 2877
PdmI GAANNNNTTC 1 cut(s) 2934
PfeI GAWTC 2 cut(s) 556, 695
PflMI CCANNNNNTGG 2 cut(s) 656, 2468
PfoI TCCNGGA 1 cut(s) 18
PinAI ACCGGT 1 cut(s) 1883
PkrI GCNGC 3 cut(s) 58, 934, 2905
PleI GAGTC 2 cut(s) 1113, 2145
PpsI GAGTC 2 cut(s) 1113, 2145
PpuMI RGGWCCY 1 cut(s) 1744
PshBI ATTAAT 1 cut(s) 1787
PsiI TTATAA 1 cut(s) 1329
Psp5II RGGWCCY 1 cut(s) 1744
Psp6I CCWGG 4 cut(s) 18, 1549, 1822, 2646
PspEI GGTNACC 1 cut(s) 1879
PspFI CCCAGC 1 cut(s) 1859
PspGI CCWGG 4 cut(s) 18, 1549, 1822, 2646
PspN4I GGNNCC 5 cut(s) 763, 1216, 1746, 1812, 2842
PspPI GGNCC 3 cut(s) 1744, 1961, 2945
PspPPI RGGWCCY 1 cut(s) 1744
PsrI GAACNNNNNNTAC 2 cut(s) 160, 192
PstI CTGCAG 3 cut(s) 879, 1051, 2019
PstNI CAGNNNCTG 3 cut(s) 1316, 2102, 2861
PsuI RGATCY 1 cut(s) 1810
PvuII CAGCTG 2 cut(s) 653, 935
RsaI GTAC 3 cut(s) 965, 1411, 1872
RsaNI GTAC 3 cut(s) 964, 1410, 1871
RseI CAYNNNNRTG 3 cut(s) 374, 2057, 2729
SapI GCTCTTC 1 cut(s) 1551
SatI GCNGC 3 cut(s) 57, 933, 2904
Sau3AI GATC 7 cut(s) 1810, 2030, 2351, 2496, 2736, 2757, 2911
Sau96I GGNCC 3 cut(s) 1744, 1961, 2945
SchI GAGTC 2 cut(s) 1114, 2145
ScrFI CCNGG 4 cut(s) 20, 1551, 1824, 2648
SduI GDGCHC 2 cut(s) 1184, 2783
SfcI CTRYAG 7 cut(s) 502, 875, 1047, 1428, 1953, 2015, 2427
SgrAI CRCCGGYG 1 cut(s) 1883
SinI GGWCC 1 cut(s) 1744
SmiMI CAYNNNNRTG 3 cut(s) 374, 2057, 2729
SmlI CTYRAG 1 cut(s) 2612
SmoI CTYRAG 1 cut(s) 2612
SphI GCATGC 2 cut(s) 1447, 2343
SsiI CCGC 1 cut(s) 2438
SspI AATATT 4 cut(s) 459, 2308, 2479, 2512
SspMI CTAG 5 cut(s) 215, 567, 794, 1188, 1752
StyD4I CCNGG 4 cut(s) 18, 1549, 1822, 2646
StyI CCWWGG 1 cut(s) 180
TaaI ACNGT 8 cut(s) 101, 1402, 1870, 2302, 2633, 2746, 2797, 2918
TaiI ACGT 1 cut(s) 2575
TaqI TCGA 3 cut(s) 72, 1500, 1567
TaqII GACCGA 1 cut(s) 2507
TfiI GAWTC 2 cut(s) 556, 695
TseFI GTSAC 8 cut(s) 484, 497, 937, 1096, 1136, 1259, 1610, 1879
TseI GCWGC 3 cut(s) 56, 932, 2903
Tsp45I GTSAC 8 cut(s) 484, 497, 937, 1096, 1136, 1259, 1610, 1879
TspDTI ATGAA 9 cut(s) 58, 614, 708, 1025, 1136, 2468, 2476, 2611, 2713
TspGWI ACGGA 2 cut(s) 1888, 2930
Van91I CCANNNNNTGG 2 cut(s) 656, 2468
VpaK11BI GGWCC 1 cut(s) 1744
VspI ATTAAT 1 cut(s) 1787
XagI CCTNNNNNAGG 1 cut(s) 318
XapI RAATTY 3 cut(s) 1436, 2483, 2771
XceI RCATGY 3 cut(s) 1170, 1447, 2343
XmnI GAANNNNTTC 1 cut(s) 2934
XspI CTAG 5 cut(s) 215, 567, 794, 1188, 1752
Zsp2I ATGCAT 1 cut(s) 1021
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.