Rorug01G0026100

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001189
Physical Location & Seq
Forward (+)
4466605 .. 4470331
3727 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0026100.1

Sequence Viewer

Length: 759 bp
ATGATGAGGGACGTGTCGACCTGCAACACGTACGATTACGGAGACGCCCAATACTGGGACGCCCGGTACGTCCAAGAAGGTGACTCCTTATTCGACTGGTACCAGCGCTACTCTGCTCTCCGCCCTTTCATCCGCAACTTCATTCCCCTCACCTCCTCTCCTGTTCTCGTCGTCGGCTCCGGCAATGCCGTTATGTCCGAGGACATGGCGAAAGATGGATATGAAGACATAATGAACATTGACATCTCATCAGTGGCTATCAACCTCATGAAGACCAGAAACAAGCACATCCCTCAGTTGAAATACAAGCAAATGGATGTGAGGGATATGAGCTTCTTCCCAGATGAATCCTTTGACGGTATTATTGATAAAGGAACTCTTGATTCATTGATGTGTGGCAGTGATGCTCCGATTAGTGCTGCCCGAATGCTAGGGGAAGTGAGTAGGCTTCTTAAACCTGGAGGGGTCTATATTTTGATAACTTATGGCGATCCTAAAGTTAGAATGCCTCATTTGAGCCGGCCAGTGTACGACTGGAATACTGTTCTGTATATCCAACCCAGACCAGGATTCCAGAGGCCTGAAGGCTGCAGTTCCTCAAGGAAGTCATATTTGGAACCTGTTCCTATCACCGAGAACGGTTCCCTTCCAGCTGAGCTTGTTATGGAAGATCCAGATTCACACTTCATTTATGTGTGTAAAAAGAAGGATGCCAAGGAATTACTTTCACTATATAGATTGAGAATTGATGATTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009705 GO:0010035 GO:0010941 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055081 GO:0055085 GO:0060548 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098542 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase Hydrolase_3

Protein Analysis

252

Amino Acids

28.74

Weight (kDa)

5.18

Isoelectric Point (pI)

44.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_31 PF13847 54 - 163 9.4e-11 Methyltransferase domain
Methyltransf_25 PF13649 55 - 155 3.3e-11 Methyltransferase domain
Methyltransf_11 PF08241 57 - 158 3e-13 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 29
Acc65I GGTACC 1 cut(s) 99
AccB1I GGYRCC 1 cut(s) 99
AccI GTMKAC 1 cut(s) 17
AciI CCGC 2 cut(s) 121, 133
AclWI GGATC 2 cut(s) 485, 665
AcoI YGGCCR 1 cut(s) 521
AcuI CTGAAG 1 cut(s) 603
AcyI GRCGYC 2 cut(s) 45, 60
AfaI GTAC 4 cut(s) 32, 68, 101, 530
AfeI AGCGCT 1 cut(s) 107
AfiI CCNNNNNNNGG 3 cut(s) 54, 55, 566
AflIII ACRYGT 2 cut(s) 12, 27
AgsI TTSAA 1 cut(s) 301
AjiI CACGTC 1 cut(s) 13
AjnI CCWGG 2 cut(s) 457, 565
AjuI GAANNNNNNNTTGG 2 cut(s) 596, 628
AluBI AGCT 3 cut(s) 333, 653, 658
AluI AGCT 3 cut(s) 333, 653, 658
Alw26I GTCTC 1 cut(s) 36
AlwI GGATC 2 cut(s) 485, 665
Aor51HI AGCGCT 1 cut(s) 107
AoxI GGCC 2 cut(s) 521, 578
ApeKI GCWGC 2 cut(s) 419, 588
ArsI GACNNNNNNTTYG 2 cut(s) 74, 106
Asp700I GAANNNNTTC 1 cut(s) 621
Asp718I GGTACC 1 cut(s) 99
AspLEI GCGC 1 cut(s) 108
AsuC2I CCSGG 1 cut(s) 64
AsuHPI GGTGA 3 cut(s) 92, 142, 622
BanI GGYRCC 1 cut(s) 99
BbsI GAAGAC 2 cut(s) 231, 278
BbvI GCAGC 2 cut(s) 406, 575
BccI CCATC 1 cut(s) 209
BceAI ACGGC 1 cut(s) 173
BciT130I CCWGG 2 cut(s) 459, 567
BcnI CCSGG 1 cut(s) 64
BcoDI GTCTC 1 cut(s) 36
BfaI CTAG 1 cut(s) 431
BfmI CTRYAG 1 cut(s) 589
BfoI RGCGCY 1 cut(s) 109
BfuAI ACCTGC 1 cut(s) 29
BisI GCNGC 2 cut(s) 420, 589
BlpI GCTNAGC 1 cut(s) 654
BlsI GCNGC 2 cut(s) 421, 590
Bme1390I CCNGG 3 cut(s) 64, 459, 567
BmgBI CACGTC 1 cut(s) 13
BmiI GGNNCC 4 cut(s) 101, 178, 618, 643
BmrFI CCNGG 3 cut(s) 64, 459, 567
BmrI ACTGGG 1 cut(s) 64
BmsI GCATC 2 cut(s) 394, 700
BmuI ACTGGG 1 cut(s) 64
BpiI GAAGAC 2 cut(s) 231, 278
BpmI CTGGAG 1 cut(s) 480
Bpu1102I GCTNAGC 1 cut(s) 654
BpuEI CTTGAG 1 cut(s) 583
BpuMI CCSGG 1 cut(s) 64
BsaAI YACGTR 1 cut(s) 30
BsaHI GRCGYC 2 cut(s) 45, 60
BsaJI CCNNGG 2 cut(s) 198, 714
BsaXI ACNNNNNCTCC 2 cut(s) 142, 172
Bsc4I CCNNNNNNNGG 3 cut(s) 54, 55, 566
Bse118I RCCGGY 1 cut(s) 519
Bse1I ACTGG 4 cut(s) 59, 101, 524, 539
Bse3DI GCAATG 1 cut(s) 190
BseBI CCWGG 2 cut(s) 459, 567
BseDI CCNNGG 2 cut(s) 198, 714
BseGI GGATG 4 cut(s) 129, 288, 322, 715
BseLI CCNNNNNNNGG 3 cut(s) 54, 55, 566
BseMI GCAATG 1 cut(s) 190
BseMII CTCAG 2 cut(s) 308, 645
BseNI ACTGG 4 cut(s) 59, 101, 524, 539
BseRI GAGGAG 1 cut(s) 145
BseXI GCAGC 2 cut(s) 406, 575
BshFI GGCC 2 cut(s) 523, 580
BshNI GGYRCC 1 cut(s) 99
BsiSI CCGG 3 cut(s) 64, 180, 520
BsiWI CGTACG 1 cut(s) 30
BslFI GGGAC 2 cut(s) 23, 71
BslI CCNNNNNNNGG 3 cut(s) 54, 55, 566
BsmAI GTCTC 1 cut(s) 36
BsmBI CGTCTC 1 cut(s) 36
BsmFI GGGAC 2 cut(s) 23, 71
BsmI GAATGC 2 cut(s) 432, 510
BsnI GGCC 2 cut(s) 523, 580
Bsp143I GATC 2 cut(s) 490, 670
Bsp1720I GCTNAGC 1 cut(s) 654
BspACI CCGC 2 cut(s) 121, 133
BspANI GGCC 2 cut(s) 523, 580
BspCNI CTCAG 2 cut(s) 307, 646
BspHI TCATGA 1 cut(s) 267
BspLI GGNNCC 4 cut(s) 101, 178, 618, 643
BspMAI CTGCAG 1 cut(s) 593
BspMI ACCTGC 1 cut(s) 29
BspPI GGATC 2 cut(s) 485, 665
BspT107I GGYRCC 1 cut(s) 99
BsrDI GCAATG 1 cut(s) 190
BsrFI RCCGGY 1 cut(s) 519
BsrI ACTGG 4 cut(s) 59, 101, 524, 539
BssAI RCCGGY 1 cut(s) 519
BssECI CCNNGG 2 cut(s) 198, 714
BssMI GATC 2 cut(s) 490, 670
BssNI GRCGYC 2 cut(s) 45, 60
BssT1I CCWWGG 1 cut(s) 714
Bst2UI CCWGG 2 cut(s) 459, 567
Bst4CI ACNGT 3 cut(s) 359, 544, 641
BstACI GRCGYC 2 cut(s) 45, 60
BstBAI YACGTR 1 cut(s) 30
BstC8I GCNNGC 1 cut(s) 521
BstDEI CTNAG 2 cut(s) 294, 654
BstF5I GGATG 4 cut(s) 129, 288, 322, 715
BstH2I RGCGCY 1 cut(s) 109
BstHHI GCGC 1 cut(s) 108
BstKTI GATC 2 cut(s) 493, 673
BstMAI GTCTC 1 cut(s) 36
BstMBI GATC 2 cut(s) 490, 670
BstNI CCWGG 2 cut(s) 459, 567
BstSCI CCNGG 3 cut(s) 62, 457, 565
BstSFI CTRYAG 1 cut(s) 589
BstV1I GCAGC 2 cut(s) 406, 575
BstV2I GAAGAC 2 cut(s) 231, 278
BstX2I RGATCY 1 cut(s) 670
BstYI RGATCY 1 cut(s) 670
BsuRI GGCC 2 cut(s) 523, 580
BtrI CACGTC 1 cut(s) 13
BtsCI GGATG 4 cut(s) 129, 288, 322, 715
BtsI GCAGTG 1 cut(s) 406
BtsIMutI CAGTG 3 cut(s) 258, 406, 531
BveI ACCTGC 1 cut(s) 29
Cac8I GCNNGC 1 cut(s) 521
CciI TCATGA 1 cut(s) 267
CfoI GCGC 1 cut(s) 108
Cfr10I RCCGGY 1 cut(s) 519
CseI GACGC 2 cut(s) 53, 68
Csp6I GTAC 4 cut(s) 31, 67, 100, 529
CviAII CATG 2 cut(s) 205, 268
CviQI GTAC 4 cut(s) 31, 67, 100, 529
DdeI CTNAG 2 cut(s) 294, 654
DpnI GATC 2 cut(s) 492, 672
DpnII GATC 2 cut(s) 490, 670
EaeI YGGCCR 1 cut(s) 521
EciI GGCGGA 1 cut(s) 110
Eco130I CCWWGG 1 cut(s) 714
Eco147I AGGCCT 1 cut(s) 580
Eco47III AGCGCT 1 cut(s) 107
Eco57I CTGAAG 1 cut(s) 603
EcoRII CCWGG 2 cut(s) 457, 565
EcoT14I CCWWGG 1 cut(s) 714
ErhI CCWWGG 1 cut(s) 714
Esp3I CGTCTC 1 cut(s) 36
FaeI CATG 2 cut(s) 208, 271
FalI AAGNNNNNCTT 2 cut(s) 363, 395
FaqI GGGAC 2 cut(s) 23, 71
FatI CATG 2 cut(s) 204, 267
FblI GTMKAC 1 cut(s) 17
Fnu4HI GCNGC 2 cut(s) 420, 589
FokI GGATG 4 cut(s) 116, 275, 329, 722
Fsp4HI GCNGC 2 cut(s) 420, 589
FspBI CTAG 1 cut(s) 431
GlaI GCGC 1 cut(s) 107
GluI GCNGC 2 cut(s) 420, 589
GsuI CTGGAG 1 cut(s) 480
HaeII RGCGCY 1 cut(s) 109
HaeIII GGCC 2 cut(s) 523, 580
HapII CCGG 3 cut(s) 64, 180, 520
HgaI GACGC 2 cut(s) 53, 68
HhaI GCGC 1 cut(s) 108
Hin1I GRCGYC 2 cut(s) 45, 60
Hin1II CATG 2 cut(s) 208, 271
Hin6I GCGC 1 cut(s) 106
HinP1I GCGC 1 cut(s) 106
HincII GTYRAC 1 cut(s) 18
HindII GTYRAC 1 cut(s) 18
HinfI GANTC 5 cut(s) 83, 347, 383, 570, 677
HpaII CCGG 3 cut(s) 64, 180, 520
HphI GGTGA 3 cut(s) 92, 142, 622
Hpy166II GTNNAC 2 cut(s) 18, 529
Hpy188I TCNGA 2 cut(s) 199, 411
Hpy188III TCNNGA 4 cut(s) 268, 380, 574, 674
Hpy8I GTNNAC 2 cut(s) 18, 529
Hpy99I CGWCG 2 cut(s) 173, 176
HpyAV CCTTC 4 cut(s) 71, 578, 656, 700
HpyCH4III ACNGT 3 cut(s) 359, 544, 641
HpyCH4IV ACGT 3 cut(s) 12, 29, 69
HpyCH4V TGCA 2 cut(s) 24, 591
HpyF3I CTNAG 2 cut(s) 294, 654
HpySE526I ACGT 3 cut(s) 12, 29, 69
Hsp92I GRCGYC 2 cut(s) 45, 60
Hsp92II CATG 2 cut(s) 208, 271
HspAI GCGC 1 cut(s) 106
KpnI GGTACC 1 cut(s) 103
KroI GCCGGC 1 cut(s) 519
KroNI GCCGGC 1 cut(s) 521
Kzo9I GATC 2 cut(s) 490, 670
LmnI GCTCC 2 cut(s) 182, 412
Lsp1109I GCAGC 2 cut(s) 406, 575
LweI GCATC 2 cut(s) 394, 700
MaeI CTAG 1 cut(s) 431
MaeII ACGT 3 cut(s) 12, 29, 69
MaeIII GTNAC 1 cut(s) 80
MalI GATC 2 cut(s) 492, 672
MboI GATC 2 cut(s) 490, 670
MboII GAAGA 4 cut(s) 236, 283, 328, 680
MflI RGATCY 1 cut(s) 670
MluCI AATT 2 cut(s) 719, 744
MlyI GAGTC 1 cut(s) 77
MmeI TCCRAC 1 cut(s) 580
MroNI GCCGGC 1 cut(s) 519
MroXI GAANNNNTTC 1 cut(s) 621
MseI TTAA 1 cut(s) 453
MslI CAYNNNNRTG 2 cut(s) 391, 692
MspA1I CMGCKG 1 cut(s) 653
MspI CCGG 3 cut(s) 64, 180, 520
MspR9I CCNGG 3 cut(s) 64, 459, 567
Mva1269I GAATGC 2 cut(s) 432, 510
MvaI CCWGG 2 cut(s) 459, 567
NaeI GCCGGC 1 cut(s) 521
NciI CCSGG 1 cut(s) 64
NdeII GATC 2 cut(s) 490, 670
NgoMIV GCCGGC 1 cut(s) 519
NlaIII CATG 2 cut(s) 208, 271
NlaIV GGNNCC 4 cut(s) 101, 178, 618, 643
NmuCI GTSAC 1 cut(s) 80
PagI TCATGA 1 cut(s) 267
PceI AGGCCT 1 cut(s) 580
PcsI WCGNNNNNNNCGW 1 cut(s) 66
PctI GAATGC 2 cut(s) 432, 510
PdiI GCCGGC 1 cut(s) 521
PdmI GAANNNNTTC 1 cut(s) 621
PfeI GAWTC 4 cut(s) 347, 383, 570, 677
Pfl23II CGTACG 1 cut(s) 30
PkrI GCNGC 2 cut(s) 421, 590
PleI GAGTC 1 cut(s) 77
PpsI GAGTC 1 cut(s) 77
Ppu21I YACGTR 1 cut(s) 30
Psp6I CCWGG 2 cut(s) 457, 565
PspGI CCWGG 2 cut(s) 457, 565
PspLI CGTACG 1 cut(s) 30
PspN4I GGNNCC 4 cut(s) 101, 178, 618, 643
PstI CTGCAG 1 cut(s) 593
PsuI RGATCY 1 cut(s) 670
PvuII CAGCTG 1 cut(s) 653
RsaI GTAC 4 cut(s) 32, 68, 101, 530
RsaNI GTAC 4 cut(s) 31, 67, 100, 529
RseI CAYNNNNRTG 2 cut(s) 391, 692
SalI GTCGAC 1 cut(s) 16
SaqAI TTAA 1 cut(s) 453
SatI GCNGC 2 cut(s) 420, 589
Sau3AI GATC 2 cut(s) 490, 670
SchI GAGTC 1 cut(s) 77
ScrFI CCNGG 3 cut(s) 64, 459, 567
SfaNI GCATC 2 cut(s) 394, 700
SfcI CTRYAG 1 cut(s) 589
SmiMI CAYNNNNRTG 2 cut(s) 391, 692
SmlI CTYRAG 1 cut(s) 598
SmoI CTYRAG 1 cut(s) 598
Sse9I AATT 2 cut(s) 719, 744
SseBI AGGCCT 1 cut(s) 580
SsiI CCGC 2 cut(s) 121, 133
SspMI CTAG 1 cut(s) 431
StuI AGGCCT 1 cut(s) 580
StyD4I CCNGG 3 cut(s) 62, 457, 565
StyI CCWWGG 1 cut(s) 714
TaaI ACNGT 3 cut(s) 359, 544, 641
TaiI ACGT 3 cut(s) 15, 32, 72
TaqI TCGA 2 cut(s) 17, 93
TasI AATT 2 cut(s) 719, 744
TfiI GAWTC 4 cut(s) 347, 383, 570, 677
Tru1I TTAA 1 cut(s) 453
Tru9I TTAA 1 cut(s) 453
TscAI CASTG 3 cut(s) 258, 406, 531
TseFI GTSAC 1 cut(s) 80
TseI GCWGC 2 cut(s) 419, 588
Tsp45I GTSAC 1 cut(s) 80
TspDTI ATGAA 8 cut(s) 118, 130, 237, 248, 284, 360, 375, 676
TspGWI ACGGA 1 cut(s) 54
TspRI CASTG 3 cut(s) 258, 406, 531
XcmI CCANNNNNNNNNTGG 1 cut(s) 531
XmiI GTMKAC 1 cut(s) 17
XmnI GAANNNNTTC 1 cut(s) 621
XspI CTAG 1 cut(s) 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.