RLG00000034773

zinc finger CCCH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0001189
Physical Location & Seq
Reverse (-)
54240809 .. 54243728
2920 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034773

Sequence Viewer

Length: 1830 bp
ATGGATTCATACGAAGCTACCAAGATCGTTTTCTCAAGGATCCAGAGCTTGGATTCAGAAAATGCATCAAAAATCATGGGGTACCTTTTGATTCAAGACCAGGGTGAGAAGGAGATGATACGCTTGGCATTTGGACCAGACACTCTGCTTCACAATCTCATCATCAATGCCAAAACCCAACTGGGTCTTGTTCACTCCAAAACTCCATCTTTCAACCCCATTTCCAGGCCCAACTCACTTTCTCTTTCATCTTCTCCAACCTCAAATCTTTGGAGCCTCAGCAACCCCATTAGTCCCAGCTCCACTTGTTCACCTACTTATGCCAATGTTGTTAGCAAAAACACTAACCTTGCTTCTGGGTCTTTATCGCCATCCATCAATGACTCTTCTAGTGCTACTGAGCTTGTTGACAACTTCCAGTTTTCTTTCCTCAATGATCCGAAGACGGACGAGGAGTTCTTCGATGGAGGCCCTGAAGGTGTTTATGGTAATTCCTCGATGAACAAGCAGAGCTACTCTGCTCACTGCGGGTGTGCTTTTGGTTCTGAAGATGTGAATTCCGGGCCCGGATGGAAACCATGTTCGTATTTTGCAAGAGGGTTCTGTAAAAATGGAAGTAGTTGCCGTTTTCTCCACAATGATGGCTTGCCTGGAAATGTCAGTATGCTTGAGCAGTGTGAAGATTTGCTCAGATCCAATGCTGCTGCCCAGCAACACAAACTTGCTGCAGTGTCACAGTTCATGTCCAACGGTTCTTCTTTGCCTTACAACAGGTGTCTCAACTTTCTTATGCAACAACAAAATGACACCCAAAGGTCATTGATGATGGGAGAAGAGCTTCACAAGTTTGGGAGATACCAACATGAGAGAAATGATATTTCGAGTTTGGGTGGAAATGTAATTCCCAGTTCAAGGCAGATCTACTTGACATTCCCAGCTGATAGTACATTTAGAGAAGAAGATGTCTCTAATTATTTCAACATGTTTGGACCTGTGCAAGATGTTAGGATTCCATACCAGCAGAAGAGAATGTTTGGGTTCGTTACATTTGTATATTCAGAAACTGTGAGGATCATTTTGGCCAAAGGGAACCCTCATTTTGTATGTGATTCTCGGGTGCTTGTGAAGCCTTATAAGGAGAAGGGGAAAATACCAGACAAGAAGCTACACCAACATTTGGAGAGGGGAGACTATTCTCTGTGTTCAAGCCTATCTGGGCTTGATTCTAGGGAGCCTTATGACTTCAATCTTGGATCAAGAATGTATTACAGTACCCAAGAGATGCTGTTGCGAAGGAAATTAGAGGACCAAGCTAACTTGGAGCAAGCTATTGAACTCCAAGAAAGAAGACTCATGAATATTCAACTTCTAGGCCTCAAGAATAACAATCATCATCAATACTATCAAGGTCTATCAGGAGGATCTCCATTTCCCTCCCCAACGTTATCACATTCACCTAAAAATCAACCCCTCGCAGATGTCTCAGAAGCCTCAGTCATTTCCCAAAGTTATGCTTTAGATGGTGAACAGCAAGAAGAGGAGAAGAGTGGCAAGGACCAATCCAATGCTGAAGAGTGTGACCTCCATCAAAGTGTGGAGCACATTTTCCCTGATAACCTGTTTGCTTCACCAGCAGTAGCTGGAGACCACATAAATCTCAGGTTTTCTTTTGGGTATGGAACCACTGGAATGCAACTAAGAAAGTTGAGCATGGCCGGTAATGGGGTAACTCAGACTTTTTGCTGGGAAACAATTTGGGAGCCAGAAAATCATACCCTCATCAAAGAAACTCGTTGGACAAGATCTAAGCAAGATCACCATCATCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1 zf-CCCH

Protein Analysis

610

Amino Acids

68.29

Weight (kDa)

5.97

Isoelectric Point (pI)

54.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PABC_AtC3H46 PF23182 1 - 65 2e-34 AtC3H46 PABC domain
zf-CCCH PF00642 193 - 213 8.1e-06 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1134
Acc65I GGTACC 1 cut(s) 81
AccB1I GGYRCC 1 cut(s) 81
AccB7I CCANNNNNTGG 2 cut(s) 49, 1177
AciI CCGC 1 cut(s) 528
AclI AACGTT 1 cut(s) 1442
AclWI GGATC 7 cut(s) 34, 47, 431, 687, 1079, 1261, 1429
AcoI YGGCCR 2 cut(s) 1080, 1713
AcsI RAATTY 1 cut(s) 556
AcuI CTGAAG 3 cut(s) 495, 567, 1590
AfaI GTAC 3 cut(s) 83, 946, 1273
AfiI CCNNNNNNNGG 5 cut(s) 49, 225, 912, 1177, 1722
AflIII ACRYGT 1 cut(s) 981
AgsI TTSAA 8 cut(s) 95, 214, 912, 979, 1206, 1246, 1334, 1364
AjnI CCWGG 3 cut(s) 99, 224, 649
AjuI GAANNNNNNNTTGG 2 cut(s) 14, 46
AloI GAACNNNNNNTCC 4 cut(s) 440, 472, 565, 597
Alw21I GWGCWC 1 cut(s) 1602
Alw26I GTCTC 5 cut(s) 782, 970, 1182, 1486, 1638
AlwI GGATC 7 cut(s) 34, 47, 431, 687, 1079, 1261, 1429
AlwNI CAGNNNCTG 2 cut(s) 1064, 1640
Ama87I CYCGRG 1 cut(s) 1113
AoxI GGCC 6 cut(s) 227, 469, 563, 1080, 1372, 1713
ApaI GGGCCC 1 cut(s) 567
ApeKI GCWGC 3 cut(s) 701, 704, 725
ApoI RAATTY 1 cut(s) 556
Asp700I GAANNNNTTC 1 cut(s) 837
Asp718I GGTACC 1 cut(s) 81
AspS9I GGNCC 8 cut(s) 134, 228, 470, 563, 564, 989, 1306, 1555
AsuC2I CCSGG 2 cut(s) 562, 567
AsuHPI GGTGA 6 cut(s) 116, 303, 1446, 1535, 1620, 1808
AvaI CYCGRG 1 cut(s) 1113
AvaII GGWCC 4 cut(s) 134, 989, 1306, 1555
BaeGI GKGCMC 1 cut(s) 567
BalI TGGCCA 1 cut(s) 1082
BamHI GGATCC 1 cut(s) 39
BanI GGYRCC 1 cut(s) 81
BanII GRGCYC 1 cut(s) 567
BbsI GAAGAC 2 cut(s) 449, 1354
Bbv12I GWGCWC 1 cut(s) 1602
BbvCI CCTCAGC 1 cut(s) 278
BbvI GCAGC 3 cut(s) 688, 691, 712
BceAI ACGGC 1 cut(s) 609
BciT130I CCWGG 3 cut(s) 101, 226, 651
BcnI CCSGG 2 cut(s) 562, 567
BcoDI GTCTC 5 cut(s) 782, 970, 1182, 1486, 1638
BfaI CTAG 3 cut(s) 390, 1227, 1370
BfmI CTRYAG 1 cut(s) 726
BglII AGATCT 2 cut(s) 918, 1802
BisI GCNGC 3 cut(s) 702, 705, 726
BlsI GCNGC 3 cut(s) 703, 706, 727
Bme1390I CCNGG 5 cut(s) 101, 226, 562, 567, 651
Bme18I GGWCC 4 cut(s) 134, 989, 1306, 1555
BmeT110I CYCGRG 1 cut(s) 1113
BmgT120I GGNCC 8 cut(s) 134, 228, 470, 563, 564, 989, 1306, 1555
BmiI GGNNCC 8 cut(s) 41, 83, 275, 565, 1091, 1233, 1681, 1761
BmrFI CCNGG 5 cut(s) 101, 226, 562, 567, 651
BmrI ACTGGG 2 cut(s) 191, 900
BmsI GCATC 2 cut(s) 74, 1272
BmuI ACTGGG 2 cut(s) 191, 900
BpiI GAAGAC 2 cut(s) 449, 1354
BpmI CTGGAG 1 cut(s) 1662
Bpu10I CCTNAGC 1 cut(s) 278
BpuEI CTTGAG 3 cut(s) 19, 689, 1361
BpuMI CCSGG 2 cut(s) 562, 567
BsaBI GATNNNNATC 1 cut(s) 1818
BsaI GGTCTC 1 cut(s) 1638
BsaJI CCNNGG 1 cut(s) 100
Bsc4I CCNNNNNNNGG 5 cut(s) 49, 225, 912, 1177, 1722
Bse118I RCCGGY 1 cut(s) 1715
Bse1I ACTGG 4 cut(s) 186, 418, 906, 1690
Bse8I GATNNNNATC 1 cut(s) 1818
BseBI CCWGG 3 cut(s) 101, 226, 651
BseDI CCNNGG 1 cut(s) 100
BseGI GGATG 2 cut(s) 371, 575
BseJI GATNNNNATC 1 cut(s) 1818
BseLI CCNNNNNNNGG 5 cut(s) 49, 225, 912, 1177, 1722
BseMII CTCAG 7 cut(s) 292, 390, 703, 1497, 1506, 1672, 1745
BseNI ACTGG 4 cut(s) 186, 418, 906, 1690
BseRI GAGGAG 2 cut(s) 467, 1553
BseSI GKGCMC 1 cut(s) 567
BseXI GCAGC 3 cut(s) 688, 691, 712
BseYI CCCAGC 4 cut(s) 296, 708, 934, 1743
BshFI GGCC 6 cut(s) 229, 471, 565, 1082, 1374, 1715
BshNI GGYRCC 1 cut(s) 81
BsiHKAI GWGCWC 1 cut(s) 1602
BsiHKCI CYCGRG 1 cut(s) 1113
BsiSI CCGG 3 cut(s) 561, 567, 1716
BslFI GGGAC 1 cut(s) 279
BslI CCNNNNNNNGG 5 cut(s) 49, 225, 912, 1177, 1722
BsmAI GTCTC 5 cut(s) 782, 970, 1182, 1486, 1638
BsmFI GGGAC 1 cut(s) 279
BsmI GAATGC 1 cut(s) 1695
BsnI GGCC 6 cut(s) 229, 471, 565, 1082, 1374, 1715
Bso31I GGTCTC 1 cut(s) 1638
BsoBI CYCGRG 1 cut(s) 1113
Bsp120I GGGCCC 1 cut(s) 563
Bsp1286I GDGCHC 2 cut(s) 567, 1602
BspACI CCGC 1 cut(s) 528
BspANI GGCC 6 cut(s) 229, 471, 565, 1082, 1374, 1715
BspCNI CTCAG 7 cut(s) 291, 391, 702, 1496, 1505, 1671, 1744
BspHI TCATGA 1 cut(s) 1353
BspLI GGNNCC 8 cut(s) 41, 83, 275, 565, 1091, 1233, 1681, 1761
BspMAI CTGCAG 1 cut(s) 730
BspPI GGATC 7 cut(s) 34, 47, 431, 687, 1079, 1261, 1429
BspQI GCTCTTC 1 cut(s) 828
BspT107I GGYRCC 1 cut(s) 81
BspTNI GGTCTC 1 cut(s) 1638
BsrFI RCCGGY 1 cut(s) 1715
BsrI ACTGG 4 cut(s) 186, 418, 906, 1690
BssAI RCCGGY 1 cut(s) 1715
BssECI CCNNGG 1 cut(s) 100
Bst2UI CCWGG 3 cut(s) 101, 226, 651
Bst4CI ACNGT 4 cut(s) 738, 752, 1066, 1271
Bst6I CTCTTC 6 cut(s) 391, 828, 1019, 1530, 1538, 1566
BstC8I GCNNGC 2 cut(s) 647, 1326
BstDEI CTNAG 9 cut(s) 278, 399, 689, 1483, 1492, 1658, 1697, 1731, 1806
BstF5I GGATG 2 cut(s) 371, 575
BstMAI GTCTC 5 cut(s) 782, 970, 1182, 1486, 1638
BstMWI GCNNNNNNNGC 2 cut(s) 1126, 1631
BstNI CCWGG 3 cut(s) 101, 226, 651
BstNSI RCATGY 1 cut(s) 985
BstSCI CCNGG 5 cut(s) 99, 224, 560, 565, 649
BstSFI CTRYAG 1 cut(s) 726
BstSLI GKGCMC 1 cut(s) 567
BstV1I GCAGC 3 cut(s) 688, 691, 712
BstV2I GAAGAC 2 cut(s) 449, 1354
BstX2I RGATCY 5 cut(s) 39, 692, 918, 1421, 1802
BstXI CCANNNNNNTGG 1 cut(s) 641
BstYI RGATCY 5 cut(s) 39, 692, 918, 1421, 1802
BsuRI GGCC 6 cut(s) 229, 471, 565, 1082, 1374, 1715
BtsCI GGATG 2 cut(s) 371, 575
BtsI GCAGTG 3 cut(s) 523, 680, 735
BtsIMutI CAGTG 4 cut(s) 523, 680, 735, 1683
Cac8I GCNNGC 2 cut(s) 647, 1326
CaiI CAGNNNCTG 2 cut(s) 1064, 1640
CciI TCATGA 1 cut(s) 1353
Cfr10I RCCGGY 1 cut(s) 1715
Cfr13I GGNCC 8 cut(s) 134, 228, 470, 563, 564, 989, 1306, 1555
Csp6I GTAC 3 cut(s) 82, 945, 1272
CviAII CATG 7 cut(s) 76, 579, 742, 863, 982, 1354, 1711
CviQI GTAC 3 cut(s) 82, 945, 1272
DdeI CTNAG 9 cut(s) 278, 399, 689, 1483, 1492, 1658, 1697, 1731, 1806
EaeI YGGCCR 2 cut(s) 1080, 1713
Eam1104I CTCTTC 6 cut(s) 391, 828, 1019, 1530, 1538, 1566
EarI CTCTTC 6 cut(s) 391, 828, 1019, 1530, 1538, 1566
Eco147I AGGCCT 1 cut(s) 1374
Eco24I GRGCYC 1 cut(s) 567
Eco31I GGTCTC 1 cut(s) 1638
Eco47I GGWCC 4 cut(s) 134, 989, 1306, 1555
Eco57I CTGAAG 3 cut(s) 495, 567, 1590
Eco88I CYCGRG 1 cut(s) 1113
EcoO109I RGGNCCY 1 cut(s) 470
EcoRI GAATTC 1 cut(s) 556
EcoRII CCWGG 3 cut(s) 99, 224, 649
EcoT22I ATGCAT 1 cut(s) 67
EcoT38I GRGCYC 1 cut(s) 567
FaeI CATG 7 cut(s) 79, 582, 745, 866, 985, 1357, 1714
FalI AAGNNNNNCTT 2 cut(s) 1498, 1530
FaqI GGGAC 1 cut(s) 279
FatI CATG 7 cut(s) 75, 578, 741, 862, 981, 1353, 1710
FauI CCCGC 1 cut(s) 521
Fnu4HI GCNGC 3 cut(s) 702, 705, 726
FokI GGATG 2 cut(s) 358, 582
FriOI GRGCYC 1 cut(s) 567
Fsp4HI GCNGC 3 cut(s) 702, 705, 726
FspBI CTAG 3 cut(s) 390, 1227, 1370
GluI GCNGC 3 cut(s) 702, 705, 726
GsaI CCCAGC 4 cut(s) 300, 712, 938, 1747
GsuI CTGGAG 1 cut(s) 1662
HaeIII GGCC 6 cut(s) 229, 471, 565, 1082, 1374, 1715
HapII CCGG 3 cut(s) 561, 567, 1716
Hin1II CATG 7 cut(s) 79, 582, 745, 866, 985, 1357, 1714
HincII GTYRAC 1 cut(s) 409
HindII GTYRAC 1 cut(s) 409
HinfI GANTC 8 cut(s) 5, 53, 91, 383, 1009, 1109, 1223, 1350
HpaII CCGG 3 cut(s) 561, 567, 1716
HphI GGTGA 6 cut(s) 116, 303, 1446, 1535, 1620, 1808
Hpy166II GTNNAC 4 cut(s) 193, 311, 409, 1526
Hpy188I TCNGA 7 cut(s) 58, 441, 547, 692, 1060, 1486, 1734
Hpy188III TCNNGA 6 cut(s) 43, 95, 1257, 1354, 1378, 1416
Hpy8I GTNNAC 4 cut(s) 193, 311, 409, 1526
HpyAV CCTTC 4 cut(s) 103, 470, 1135, 1287
HpyCH4III ACNGT 4 cut(s) 738, 752, 1066, 1271
HpyCH4IV ACGT 1 cut(s) 1442
HpyCH4V TGCA 6 cut(s) 65, 593, 728, 793, 997, 1693
HpyF10VI GCNNNNNNNGC 2 cut(s) 1126, 1631
HpyF3I CTNAG 9 cut(s) 278, 399, 689, 1483, 1492, 1658, 1697, 1731, 1806
HpySE526I ACGT 1 cut(s) 1442
Hsp92II CATG 7 cut(s) 79, 582, 745, 866, 985, 1357, 1714
KpnI GGTACC 1 cut(s) 85
LguI GCTCTTC 1 cut(s) 828
LmnI GCTCC 6 cut(s) 273, 305, 1231, 1321, 1597, 1759
Lsp1109I GCAGC 3 cut(s) 688, 691, 712
LweI GCATC 2 cut(s) 74, 1272
MaeI CTAG 3 cut(s) 390, 1227, 1370
MaeII ACGT 1 cut(s) 1442
MaeIII GTNAC 4 cut(s) 732, 1042, 1577, 1726
MflI RGATCY 5 cut(s) 39, 692, 918, 1421, 1802
MhlI GDGCHC 2 cut(s) 567, 1602
MlsI TGGCCA 1 cut(s) 1082
MluCI AATT 6 cut(s) 490, 556, 900, 970, 1298, 1752
MluNI TGGCCA 1 cut(s) 1082
MlyI GAGTC 2 cut(s) 377, 1344
MmeI TCCRAC 3 cut(s) 281, 771, 1775
Mox20I TGGCCA 1 cut(s) 1082
Mph1103I ATGCAT 1 cut(s) 67
MroXI GAANNNNTTC 1 cut(s) 837
MscI TGGCCA 1 cut(s) 1082
MseI TTAA 1 cut(s) 1828
MslI CAYNNNNRTG 3 cut(s) 639, 1590, 1688
Msp20I TGGCCA 1 cut(s) 1082
MspA1I CMGCKG 1 cut(s) 938
MspI CCGG 3 cut(s) 561, 567, 1716
MspR9I CCNGG 5 cut(s) 101, 226, 562, 567, 651
Mva1269I GAATGC 1 cut(s) 1695
MvaI CCWGG 3 cut(s) 101, 226, 651
MwoI GCNNNNNNNGC 2 cut(s) 1126, 1631
NciI CCSGG 2 cut(s) 562, 567
NlaIII CATG 7 cut(s) 79, 582, 745, 866, 985, 1357, 1714
NlaIV GGNNCC 8 cut(s) 41, 83, 275, 565, 1091, 1233, 1681, 1761
NmuCI GTSAC 2 cut(s) 732, 1577
NsiI ATGCAT 1 cut(s) 67
NspI RCATGY 1 cut(s) 985
PagI TCATGA 1 cut(s) 1353
PceI AGGCCT 1 cut(s) 1374
PciI ACATGT 1 cut(s) 981
PciSI GCTCTTC 1 cut(s) 828
PctI GAATGC 1 cut(s) 1695
PdmI GAANNNNTTC 1 cut(s) 837
PfeI GAWTC 6 cut(s) 5, 53, 91, 1009, 1109, 1223
PflMI CCANNNNNTGG 2 cut(s) 49, 1177
PkrI GCNGC 3 cut(s) 703, 706, 727
PleI GAGTC 2 cut(s) 377, 1344
PpsI GAGTC 2 cut(s) 377, 1344
PscI ACATGT 1 cut(s) 981
PsiI TTATAA 1 cut(s) 1134
Psp1406I AACGTT 1 cut(s) 1442
Psp6I CCWGG 3 cut(s) 99, 224, 649
PspFI CCCAGC 4 cut(s) 296, 708, 934, 1743
PspGI CCWGG 3 cut(s) 99, 224, 649
PspN4I GGNNCC 8 cut(s) 41, 83, 275, 565, 1091, 1233, 1681, 1761
PspOMI GGGCCC 1 cut(s) 563
PspPI GGNCC 8 cut(s) 134, 228, 470, 563, 564, 989, 1306, 1555
PstI CTGCAG 1 cut(s) 730
PstNI CAGNNNCTG 2 cut(s) 1064, 1640
PsuI RGATCY 5 cut(s) 39, 692, 918, 1421, 1802
PvuII CAGCTG 1 cut(s) 938
RsaI GTAC 3 cut(s) 83, 946, 1273
RsaNI GTAC 3 cut(s) 82, 945, 1272
RseI CAYNNNNRTG 3 cut(s) 639, 1590, 1688
SapI GCTCTTC 1 cut(s) 828
SaqAI TTAA 1 cut(s) 1828
SatI GCNGC 3 cut(s) 702, 705, 726
Sau96I GGNCC 8 cut(s) 134, 228, 470, 563, 564, 989, 1306, 1555
SchI GAGTC 2 cut(s) 377, 1344
ScrFI CCNGG 5 cut(s) 101, 226, 562, 567, 651
SduI GDGCHC 2 cut(s) 567, 1602
SfaNI GCATC 2 cut(s) 74, 1272
SfcI CTRYAG 1 cut(s) 726
SinI GGWCC 4 cut(s) 134, 989, 1306, 1555
SmiMI CAYNNNNRTG 3 cut(s) 639, 1590, 1688
SmlI CTYRAG 3 cut(s) 34, 668, 1376
SmoI CTYRAG 3 cut(s) 34, 668, 1376
Sse9I AATT 6 cut(s) 490, 556, 900, 970, 1298, 1752
SseBI AGGCCT 1 cut(s) 1374
SsiI CCGC 1 cut(s) 528
SspI AATATT 1 cut(s) 1360
SspMI CTAG 3 cut(s) 390, 1227, 1370
StuI AGGCCT 1 cut(s) 1374
StyD4I CCNGG 5 cut(s) 99, 224, 560, 565, 649
TaaI ACNGT 4 cut(s) 738, 752, 1066, 1271
TaiI ACGT 1 cut(s) 1445
TaqI TCGA 3 cut(s) 462, 497, 881
TasI AATT 6 cut(s) 490, 556, 900, 970, 1298, 1752
TatI WGTACW 1 cut(s) 944
TfiI GAWTC 6 cut(s) 5, 53, 91, 1009, 1109, 1223
Tru1I TTAA 1 cut(s) 1828
Tru9I TTAA 1 cut(s) 1828
TscAI CASTG 4 cut(s) 530, 680, 735, 1690
TseFI GTSAC 2 cut(s) 732, 1577
TseI GCWGC 3 cut(s) 701, 704, 725
Tsp45I GTSAC 2 cut(s) 732, 1577
TspDTI ATGAA 4 cut(s) 237, 515, 730, 1370
TspGWI ACGGA 1 cut(s) 461
TspRI CASTG 4 cut(s) 530, 680, 735, 1690
Van91I CCANNNNNTGG 2 cut(s) 49, 1177
VpaK11BI GGWCC 4 cut(s) 134, 989, 1306, 1555
XapI RAATTY 1 cut(s) 556
XceI RCATGY 1 cut(s) 985
XcmI CCANNNNNNNNNTGG 1 cut(s) 178
XmnI GAANNNNTTC 1 cut(s) 837
XspI CTAG 3 cut(s) 390, 1227, 1370
Zsp2I ATGCAT 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.