MD13G1017100.v1.1

zinc finger CCCH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0001189
Physical Location & Seq
Reverse (-)
1111783 .. 1112916
1134 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1017100.v1.1.491

Sequence Viewer

Length: 1134 bp
ATGGAGGACCCCAACTCAGGGTTTGGGTGGAAACCCTGCCTGTATTTCGCCAGAAGCTACTGCAAGAACGGAGCCAGCTGCAGGTTCTTGCACAGCGGTGGGCTAGGAGACGCCGTCGTCGACGGCAGAGGACAGGTGGTTGTTGGGTCGCCGAGCAAGCTGGAGATGATGGACCATGAAGCGCTTCTCAGATCTAAAACCGCTCAGCAGCAGAGGCTTGCTGCTGCTTCTCAGCTTATGGCAGCGTCAGCCAATTCCTTTCCTTACTCCTCCAAGTGCATGAACTTCCTACTTCAGCAGCAACAAACTGATTCCCAAAGGGCTGCGGCAGCTTCCTTGATGATGGGTGATGATATGCACAAGTTCAGCCGATCCCGCCTCGAAAGATCCGATTTTTCGATGAATGGTGGGGCGGGGATTGTGAACCCGGCTTCAAGGCAGATATATTTGACTTTCCCGGCAAATAGCACTTTCAGAGAGGAAGATGTCTCAAACTATTTCAGCATGTACGGGCCGGTCCAAGACGTGAGAATCCCATACCAGCAGAAGAGGATGTTTGGATTCGTTACATTCTTGTACCCGGAGACGGTGAAGCTGATTTTGGCCAAAGGGAACCCTCATTTTGTTTGTGATGCTAGAGTGCTTGTCAAGCCTTACAAGGAGAAGGGCAAAGCTCATAAAAAGAAACAACAGCAACAAGTGGAAAGAGGAGATTTGTCTCCCTGTGGTACACCTACTGGGCTGGATGGTAGAGACCCTTACGATCTCCAGCTAGGAGCAAAGATGTTTTACAATTCTCAAGACATGCTGTGGAGGAGGAAGCTGGAGGAGCAAGCTGAGTTGCAAGCACTTGAAATTCAAAGTCGAAGACTGATGGGCCTGCAGCTACATGATGTCAAGAAGCATCACCACCACGCTCTTTCTGCCAGCAGTCCGATTCACTCCCCTACCCAGTCTCCCAACATGTTCAGTCGAAACCTAGTGCTTCATTCGGTTCACAACGGCCAAGAAGTTCACCACGATAACTGTTTTTCTCCGATGCCACACTTGACTCCAGCAGTGGATGCTGATCAGTTGCTCAAGGAAGCAGCTGCTGCTGTTCTAGGTAAAGATATGATGGCCAACAACGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1 Torus zf-CCCH

Protein Analysis

378

Amino Acids

42.14

Weight (kDa)

8.84

Isoelectric Point (pI)

57.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 116
Acc36I ACCTGC 1 cut(s) 72
AccBSI CCGCTC 1 cut(s) 203
AccI GTMKAC 1 cut(s) 120
AciI CCGC 5 cut(s) 96, 201, 326, 376, 413
AclWI GGATC 2 cut(s) 366, 381
AcoI YGGCCR 3 cut(s) 603, 1003, 1119
AcsI RAATTY 1 cut(s) 855
AcuI CTGAAG 1 cut(s) 278
AcyI GRCGYC 1 cut(s) 111
AfaI GTAC 3 cut(s) 509, 578, 730
AfeI AGCGCT 1 cut(s) 183
AfiI CCNNNNNNNGG 4 cut(s) 17, 18, 81, 586
AflIII ACRYGT 1 cut(s) 963
AgsI TTSAA 3 cut(s) 435, 854, 860
AjiI CACGTC 1 cut(s) 526
AjuI GAANNNNNNNTTGG 2 cut(s) 584, 616
AleI CACNNNNGTG 1 cut(s) 96
Alw26I GTCTC 6 cut(s) 102, 493, 578, 723, 747, 960
AlwI GGATC 2 cut(s) 366, 381
AlwNI CAGNNNCTG 1 cut(s) 1094
Aor51HI AGCGCT 1 cut(s) 183
AoxI GGCC 5 cut(s) 512, 603, 877, 1003, 1119
ApoI RAATTY 1 cut(s) 855
Asp700I GAANNNNTTC 1 cut(s) 183
AspLEI GCGC 1 cut(s) 184
AspS9I GGNCC 5 cut(s) 7, 172, 512, 517, 877
AsuC2I CCSGG 3 cut(s) 428, 458, 581
AsuHPI GGTGA 4 cut(s) 359, 601, 899, 1007
AvaII GGWCC 3 cut(s) 7, 172, 517
BalI TGGCCA 2 cut(s) 605, 1121
BbsI GAAGAC 1 cut(s) 874
BccI CCATC 5 cut(s) 163, 337, 740, 868, 1111
BceAI ACGGC 3 cut(s) 98, 139, 1018
BclI TGATCA 1 cut(s) 1069
BcnI CCSGG 3 cut(s) 428, 458, 581
BcoDI GTCTC 6 cut(s) 102, 493, 578, 723, 747, 960
BfaI CTAG 5 cut(s) 104, 636, 773, 980, 1103
BfmI CTRYAG 2 cut(s) 79, 881
BfoI RGCGCY 1 cut(s) 185
BfuAI ACCTGC 1 cut(s) 72
BglII AGATCT 1 cut(s) 191
BlpI GCTNAGC 1 cut(s) 204
Bme1390I CCNGG 3 cut(s) 428, 458, 581
Bme18I GGWCC 3 cut(s) 7, 172, 517
BmgBI CACGTC 1 cut(s) 526
BmgT120I GGNCC 5 cut(s) 7, 172, 512, 517, 877
BmiI GGNNCC 3 cut(s) 9, 73, 614
BmrFI CCNGG 3 cut(s) 428, 458, 581
BmrI ACTGGG 2 cut(s) 747, 946
BmsI GCATC 4 cut(s) 622, 913, 1029, 1054
BmuI ACTGGG 2 cut(s) 747, 946
BpiI GAAGAC 1 cut(s) 874
BpmI CTGGAG 4 cut(s) 182, 752, 845, 1038
Bpu1102I GCTNAGC 1 cut(s) 204
BpuEI CTTGAG 2 cut(s) 783, 1064
BpuMI CCSGG 3 cut(s) 428, 458, 581
BsaBI GATNNNNATC 1 cut(s) 1068
BsaHI GRCGYC 1 cut(s) 111
BsaI GGTCTC 1 cut(s) 747
BsaXI ACNNNNNCTCC 4 cut(s) 99, 129, 940, 970
Bsc4I CCNNNNNNNGG 4 cut(s) 17, 18, 81, 586
Bse118I RCCGGY 1 cut(s) 514
Bse1I ACTGG 2 cut(s) 742, 952
Bse8I GATNNNNATC 1 cut(s) 1068
BseGI GGATG 3 cut(s) 558, 751, 1069
BseJI GATNNNNATC 1 cut(s) 1068
BseLI CCNNNNNNNGG 4 cut(s) 17, 18, 81, 586
BseMII CTCAG 5 cut(s) 30, 202, 218, 245, 828
BseNI ACTGG 2 cut(s) 742, 952
BseRI GAGGAG 4 cut(s) 259, 723, 829, 842
BshFI GGCC 5 cut(s) 514, 605, 879, 1005, 1121
BsiSI CCGG 4 cut(s) 428, 458, 515, 581
BslI CCNNNNNNNGG 4 cut(s) 17, 18, 81, 586
BsmAI GTCTC 6 cut(s) 102, 493, 578, 723, 747, 960
BsmBI CGTCTC 2 cut(s) 102, 578
BsnI GGCC 5 cut(s) 514, 605, 879, 1005, 1121
Bso31I GGTCTC 1 cut(s) 747
Bsp143I GATC 5 cut(s) 191, 371, 386, 763, 1069
Bsp1720I GCTNAGC 1 cut(s) 204
BspACI CCGC 5 cut(s) 96, 201, 326, 376, 413
BspANI GGCC 5 cut(s) 514, 605, 879, 1005, 1121
BspCNI CTCAG 5 cut(s) 29, 201, 217, 244, 829
BspLI GGNNCC 3 cut(s) 9, 73, 614
BspMAI CTGCAG 2 cut(s) 83, 885
BspMI ACCTGC 1 cut(s) 72
BspPI GGATC 2 cut(s) 366, 381
BspTNI GGTCTC 1 cut(s) 747
BsrBI CCGCTC 1 cut(s) 203
BsrFI RCCGGY 1 cut(s) 514
BsrI ACTGG 2 cut(s) 742, 952
BssAI RCCGGY 1 cut(s) 514
BssMI GATC 5 cut(s) 191, 371, 386, 763, 1069
BssNI GRCGYC 1 cut(s) 111
Bst4CI ACNGT 2 cut(s) 589, 1028
Bst6I CTCTTC 1 cut(s) 542
BstACI GRCGYC 1 cut(s) 111
BstAPI GCANNNNNTGC 2 cut(s) 1064, 1094
BstC8I GCNNGC 7 cut(s) 76, 158, 219, 834, 846, 881, 928
BstDEI CTNAG 5 cut(s) 16, 188, 204, 231, 837
BstF5I GGATG 3 cut(s) 558, 751, 1069
BstH2I RGCGCY 1 cut(s) 185
BstHHI GCGC 1 cut(s) 184
BstKTI GATC 5 cut(s) 194, 374, 389, 766, 1072
BstMAI GTCTC 6 cut(s) 102, 493, 578, 723, 747, 960
BstMBI GATC 5 cut(s) 191, 371, 386, 763, 1069
BstNSI RCATGY 3 cut(s) 508, 808, 967
BstSCI CCNGG 3 cut(s) 426, 456, 579
BstSFI CTRYAG 2 cut(s) 79, 881
BstV2I GAAGAC 1 cut(s) 874
BstX2I RGATCY 2 cut(s) 191, 386
BstYI RGATCY 2 cut(s) 191, 386
BsuRI GGCC 5 cut(s) 514, 605, 879, 1005, 1121
BtrI CACGTC 1 cut(s) 526
BtsCI GGATG 3 cut(s) 558, 751, 1069
BtsI GCAGTG 1 cut(s) 1065
BtsIMutI CAGTG 1 cut(s) 1065
BveI ACCTGC 1 cut(s) 72
Cac8I GCNNGC 7 cut(s) 76, 158, 219, 834, 846, 881, 928
CaiI CAGNNNCTG 1 cut(s) 1094
CfoI GCGC 1 cut(s) 184
Cfr10I RCCGGY 1 cut(s) 514
Cfr13I GGNCC 5 cut(s) 7, 172, 512, 517, 877
CseI GACGC 2 cut(s) 119, 234
Csp6I GTAC 3 cut(s) 508, 577, 729
CviAII CATG 6 cut(s) 176, 280, 505, 805, 890, 964
CviQI GTAC 3 cut(s) 508, 577, 729
DdeI CTNAG 5 cut(s) 16, 188, 204, 231, 837
DpnI GATC 5 cut(s) 193, 373, 388, 765, 1071
DpnII GATC 5 cut(s) 191, 371, 386, 763, 1069
DrdI GACNNNNNNGTC 1 cut(s) 116
DseDI GACNNNNNNGTC 1 cut(s) 116
EaeI YGGCCR 3 cut(s) 603, 1003, 1119
Eam1104I CTCTTC 1 cut(s) 542
EarI CTCTTC 1 cut(s) 542
Eco31I GGTCTC 1 cut(s) 747
Eco47I GGWCC 3 cut(s) 7, 172, 517
Eco47III AGCGCT 1 cut(s) 183
Eco57I CTGAAG 1 cut(s) 278
EcoO109I RGGNCCY 1 cut(s) 7
Esp3I CGTCTC 2 cut(s) 102, 578
FaeI CATG 6 cut(s) 179, 283, 508, 808, 893, 967
FatI CATG 6 cut(s) 175, 279, 504, 804, 889, 963
FauI CCCGC 2 cut(s) 383, 406
FbaI TGATCA 1 cut(s) 1069
FblI GTMKAC 1 cut(s) 120
FokI GGATG 3 cut(s) 565, 758, 1076
FspBI CTAG 5 cut(s) 104, 636, 773, 980, 1103
GlaI GCGC 1 cut(s) 183
GsuI CTGGAG 4 cut(s) 182, 752, 845, 1038
HaeII RGCGCY 1 cut(s) 185
HaeIII GGCC 5 cut(s) 514, 605, 879, 1005, 1121
HapII CCGG 4 cut(s) 428, 458, 515, 581
HgaI GACGC 2 cut(s) 119, 234
HhaI GCGC 1 cut(s) 184
Hin1I GRCGYC 1 cut(s) 111
Hin1II CATG 6 cut(s) 179, 283, 508, 808, 893, 967
Hin6I GCGC 1 cut(s) 182
HinP1I GCGC 1 cut(s) 182
HincII GTYRAC 1 cut(s) 121
HindII GTYRAC 1 cut(s) 121
HinfI GANTC 5 cut(s) 311, 531, 561, 937, 1051
HpaII CCGG 4 cut(s) 428, 458, 515, 581
HphI GGTGA 4 cut(s) 359, 601, 899, 1007
Hpy166II GTNNAC 5 cut(s) 121, 424, 731, 997, 1015
Hpy188I TCNGA 5 cut(s) 191, 391, 476, 936, 1038
Hpy188III TCNNGA 2 cut(s) 800, 898
Hpy8I GTNNAC 5 cut(s) 121, 424, 731, 997, 1015
Hpy99I CGWCG 3 cut(s) 119, 122, 125
HpyAV CCTTC 1 cut(s) 658
HpyCH4III ACNGT 2 cut(s) 589, 1028
HpyCH4IV ACGT 1 cut(s) 525
HpyCH4V TGCA 7 cut(s) 63, 81, 91, 279, 358, 844, 883
HpyF3I CTNAG 5 cut(s) 16, 188, 204, 231, 837
HpySE526I ACGT 1 cut(s) 525
Hsp92I GRCGYC 1 cut(s) 111
Hsp92II CATG 6 cut(s) 179, 283, 508, 808, 893, 967
HspAI GCGC 1 cut(s) 182
Ksp22I TGATCA 1 cut(s) 1069
Kzo9I GATC 5 cut(s) 191, 371, 386, 763, 1069
LmnI GCTCC 3 cut(s) 71, 776, 829
LweI GCATC 4 cut(s) 622, 913, 1029, 1054
MaeI CTAG 5 cut(s) 104, 636, 773, 980, 1103
MaeII ACGT 1 cut(s) 525
MaeIII GTNAC 1 cut(s) 565
MalI GATC 5 cut(s) 193, 373, 388, 765, 1071
MbiI CCGCTC 1 cut(s) 203
MboI GATC 5 cut(s) 191, 371, 386, 763, 1069
MboII GAAGA 3 cut(s) 494, 559, 879
MflI RGATCY 2 cut(s) 191, 386
MlsI TGGCCA 2 cut(s) 605, 1121
MluCI AATT 3 cut(s) 253, 793, 855
MluNI TGGCCA 2 cut(s) 605, 1121
MlyI GAGTC 1 cut(s) 1045
Mox20I TGGCCA 2 cut(s) 605, 1121
MroXI GAANNNNTTC 1 cut(s) 183
MscI TGGCCA 2 cut(s) 605, 1121
MslI CAYNNNNRTG 1 cut(s) 96
Msp20I TGGCCA 2 cut(s) 605, 1121
MspA1I CMGCKG 3 cut(s) 78, 96, 1091
MspI CCGG 4 cut(s) 428, 458, 515, 581
MspR9I CCNGG 3 cut(s) 428, 458, 581
NciI CCSGG 3 cut(s) 428, 458, 581
NdeII GATC 5 cut(s) 191, 371, 386, 763, 1069
NlaIII CATG 6 cut(s) 179, 283, 508, 808, 893, 967
NlaIV GGNNCC 3 cut(s) 9, 73, 614
NmeAIII GCCGAG 1 cut(s) 177
NspI RCATGY 3 cut(s) 508, 808, 967
OliI CACNNNNGTG 1 cut(s) 96
PciI ACATGT 1 cut(s) 963
PcsI WCGNNNNNNNCGW 2 cut(s) 117, 387
PdmI GAANNNNTTC 1 cut(s) 183
PfeI GAWTC 4 cut(s) 311, 531, 561, 937
PflFI GACNNNGTC 1 cut(s) 113
PleI GAGTC 1 cut(s) 1045
PpsI GAGTC 1 cut(s) 1045
PpuMI RGGWCCY 1 cut(s) 7
PscI ACATGT 1 cut(s) 963
Psp5II RGGWCCY 1 cut(s) 7
PspN4I GGNNCC 3 cut(s) 9, 73, 614
PspPI GGNCC 5 cut(s) 7, 172, 512, 517, 877
PspPPI RGGWCCY 1 cut(s) 7
PstI CTGCAG 2 cut(s) 83, 885
PstNI CAGNNNCTG 1 cut(s) 1094
PsuI RGATCY 2 cut(s) 191, 386
PsyI GACNNNGTC 1 cut(s) 113
PvuII CAGCTG 2 cut(s) 78, 1091
RsaI GTAC 3 cut(s) 509, 578, 730
RsaNI GTAC 3 cut(s) 508, 577, 729
RseI CAYNNNNRTG 1 cut(s) 96
SalI GTCGAC 1 cut(s) 119
Sau3AI GATC 5 cut(s) 191, 371, 386, 763, 1069
Sau96I GGNCC 5 cut(s) 7, 172, 512, 517, 877
SchI GAGTC 1 cut(s) 1045
ScrFI CCNGG 3 cut(s) 428, 458, 581
SfaNI GCATC 4 cut(s) 622, 913, 1029, 1054
SfcI CTRYAG 2 cut(s) 79, 881
SgrDI CGTCGACG 1 cut(s) 119
SinI GGWCC 3 cut(s) 7, 172, 517
SmiMI CAYNNNNRTG 1 cut(s) 96
SmlI CTYRAG 2 cut(s) 798, 1079
SmoI CTYRAG 2 cut(s) 798, 1079
Sse9I AATT 3 cut(s) 253, 793, 855
SsiI CCGC 5 cut(s) 96, 201, 326, 376, 413
SspMI CTAG 5 cut(s) 104, 636, 773, 980, 1103
StyD4I CCNGG 3 cut(s) 426, 456, 579
TaaI ACNGT 2 cut(s) 589, 1028
TaiI ACGT 1 cut(s) 528
TaqI TCGA 5 cut(s) 120, 381, 398, 865, 973
TasI AATT 3 cut(s) 253, 793, 855
TauI GCSGC 1 cut(s) 329
TfiI GAWTC 4 cut(s) 311, 531, 561, 937
TscAI CASTG 1 cut(s) 1065
TspDTI ATGAA 4 cut(s) 192, 296, 416, 977
TspGWI ACGGA 1 cut(s) 84
TspRI CASTG 1 cut(s) 1065
Tth111I GACNNNGTC 1 cut(s) 113
VpaK11BI GGWCC 3 cut(s) 7, 172, 517
XapI RAATTY 1 cut(s) 855
XceI RCATGY 3 cut(s) 508, 808, 967
XmiI GTMKAC 1 cut(s) 120
XmnI GAANNNNTTC 1 cut(s) 183
XspI CTAG 5 cut(s) 104, 636, 773, 980, 1103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.