AT3G51950

Zinc finger (CCCH-type) family protein RNA recognition motif (RRM)-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0001189
Physical Location & Seq
Reverse (-)
19277594 .. 19281393
3800 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G51950.3

Sequence Viewer

Length: 1623 bp
ATGGATGGATATGAAGCTACTAGGATTGTGCTCTCTAGAATCCAAAGCTTAGACCCTGAAAACGCATCAAAGATCATGGGTCTTCTCCTTCTTCAAGATCACGGTGAAAAAGAGATGATAAGGCTAGCTTTTGGTCCAGAGACTCTTGTTCACTCTGTTATAGTGAAAGCCAAGAAAGAGTTAGGTCTCATGAACTGTTCAAGGTCTCCGTGGAGTCATCAAGATGAGTTGATTAGCCCTAAGAACAACCGTGGCTCTTCACTCAATCCAGCTTCTTTGCCCTTTTACGCTAATGGAGGAAGATCTTCTAGGGATTTAACCAACGATTTCGAGCTCATGGATGATATGAACTCCAGAAGTACTGATTTTTTGGGCTCTGTGCATGCGAGAAGCGGTAGCTGCGTTTTGGACGGTTTAGGGTATGGTGGTGATTCTGATTTAGGGTTTGGAGGTGTGCCCTGTTCTTACTTCGCTAGAGGCTTCTGCAAAAACGGAGCTAGCTGCAGATTCGTCCACAGTGATGGAGGAGCTGATTTGGTTGGCTCCCCAAGCAGAATCGAGCTTCTTAGGTCTAACTCGGTACCCCCAAGACTTGCTCACCACTTCATGACTCGCTCTTCTCTCCCTTCTTTTTCAACTAAAGGTGTTAACTTGCAGCAAAACGATGTTCAAAGAGCTGCTGCTGCTTTGATGATAGGAGATGAATTGCAGAAGCTTGGAAGATGGAGACCTGAAAGGATTGATCTTTCTGCTATGGCTTGTCCAGCTTCAAGACAGATCTATCTGACATTCCCTGCCGACAGTAGGTTCAGGGAGGAAGATGTGTCCAATTACTTCAGTACTTTTGGACCAGTTCAAGATGTGAGGATACCATATCAGCAAAAGAGAATGTTTGGTTTTGTGACATTTGTGTACCCTGAGACTGTTAAGAGCATTCTCGCCAAAGGGAATCCTCACTTTGTGTGTGATTCCAGAGTTCTTGTCAAGCCTTACAAGGAGAAAGGCAAAGTCCCTGACAAATACAGAACTAACCAAACAACAGAGCGAGAACTGTCCCCAACAGGCCTTGATTCTAGCCCTAGGGACGTTCTAGGAGGGAGAGGGTTTTATAACAACACTCAAGATGTGTTGTGGAGGAGTAAGTTTGAAGAAGAGATTCTTGAACTTCAGAGCAGAAGGCTGATGAATCTGCAGCTTCTTGACGTCAAGAAGCATTTCCAACTCAATTCCCCTACCAACATTCACTCTCCGAATCCTTTCAGCCAATCACTTATATCTCCACGCCCATTGTCCGTGATCAAGAGAGAGTATGATGGAGGAGAGAAAGGGAAAGGAAGTTCTAAAGAAGGATCTGATGATGATACAATGAATCTACCAGAGAGGTTGGAGGATAGCTTGCCAGATAGTCCGTTTGCATCGCCCGCTCATCATTTGCTTCTGTTTGCTGATTCTGCTGACAATAACGGATCGGATCTGTGGTCGCCTTCTTCTGATAATGATGATAATTCTACTCCTTCTACACTCTCCGACTCCTTCAACTCTTTCAACTACCAAATGCCAAGGTTACCGGCGATTGGAATGTTACCCGGTAGGGGTGGACCAACCTGTCGTGTTGGGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1 Torus zf-CCCH

Protein Analysis

540

Amino Acids

59.79

Weight (kDa)

6.48

Isoelectric Point (pI)

59.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PABC_AtC3H46 PF23182 1 - 64 1.7e-33 AtC3H46 PABC domain
zf-CCCH PF00642 151 - 173 1.9e-06 Zinc finger C-x8-C-x5-C-x3-H type (and similar)
RRM_1 PF00076 271 - 317 1.6e-06 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1110
AasI GACNNNNNNGTC 1 cut(s) 1605
AatII GACGTC 1 cut(s) 1206
Acc65I GGTACC 1 cut(s) 580
AccB1I GGYRCC 1 cut(s) 580
AccBSI CCGCTC 1 cut(s) 1424
AciI CCGC 2 cut(s) 393, 1422
AclWI GGATC 3 cut(s) 1357, 1474, 1479
AcuI CTGAAG 2 cut(s) 820, 1151
AcyI GRCGYC 1 cut(s) 1203
AdeI CACNNNGTG 1 cut(s) 961
AfaI GTAC 4 cut(s) 361, 582, 841, 914
AfiI CCNNNNNNNGG 3 cut(s) 804, 1574, 1592
AloI GAACNNNNNNTCC 2 cut(s) 1321, 1353
Alw21I GWGCWC 2 cut(s) 33, 336
Alw26I GTCTC 5 cut(s) 134, 191, 210, 721, 914
AlwI GGATC 3 cut(s) 1357, 1474, 1479
AoxI GGCC 1 cut(s) 1063
ApeKI GCWGC 7 cut(s) 399, 501, 655, 677, 680, 683, 1192
Asp700I GAANNNNTTC 4 cut(s) 304, 1155, 1214, 1256
Asp718I GGTACC 1 cut(s) 580
AspA2I CCTAGG 1 cut(s) 1079
AspS9I GGNCC 3 cut(s) 134, 848, 1598
AsuC2I CCSGG 1 cut(s) 1587
AsuHPI GGTGA 3 cut(s) 116, 440, 590
AsuNHI GCTAGC 2 cut(s) 124, 497
AvaII GGWCC 3 cut(s) 134, 848, 1598
AvrII CCTAGG 1 cut(s) 1079
BaeGI GKGCMC 1 cut(s) 459
BanI GGYRCC 1 cut(s) 580
BanII GRGCYC 2 cut(s) 336, 377
BbsI GAAGAC 1 cut(s) 74
Bbv12I GWGCWC 2 cut(s) 33, 336
BbvI GCAGC 7 cut(s) 386, 488, 664, 667, 667, 670, 1204
BccI CCATC 3 cut(s) 515, 717, 1307
BciVI GTATCC 1 cut(s) 861
BclI TGATCA 1 cut(s) 1296
BcnI CCSGG 1 cut(s) 1587
BcoDI GTCTC 5 cut(s) 134, 191, 210, 721, 914
BfaI CTAG 9 cut(s) 21, 36, 125, 309, 474, 498, 1074, 1080, 1091
BfmI CTRYAG 2 cut(s) 502, 1190
BfuI GTATCC 1 cut(s) 861
BglII AGATCT 2 cut(s) 302, 777
BisI GCNGC 7 cut(s) 400, 502, 656, 678, 681, 684, 1193
BlnI CCTAGG 1 cut(s) 1079
BlsI GCNGC 7 cut(s) 401, 503, 657, 679, 682, 685, 1194
BmcAI AGTACT 2 cut(s) 361, 841
Bme1390I CCNGG 1 cut(s) 1587
Bme18I GGWCC 3 cut(s) 134, 848, 1598
BmgT120I GGNCC 3 cut(s) 134, 848, 1598
BmiI GGNNCC 2 cut(s) 544, 582
BmrFI CCNGG 1 cut(s) 1587
BmsI GCATC 2 cut(s) 74, 1424
BmtI GCTAGC 2 cut(s) 128, 501
BpiI GAAGAC 1 cut(s) 74
BpmI CTGGAG 1 cut(s) 337
BpuEI CTTGAG 1 cut(s) 1104
BpuMI CCSGG 1 cut(s) 1587
BsaHI GRCGYC 1 cut(s) 1203
BsaI GGTCTC 3 cut(s) 191, 210, 721
BsaJI CCNNGG 4 cut(s) 209, 250, 1079, 1559
Bsc4I CCNNNNNNNGG 3 cut(s) 804, 1574, 1592
Bse118I RCCGGY 1 cut(s) 1567
Bse1I ACTGG 1 cut(s) 851
BseDI CCNNGG 4 cut(s) 209, 250, 1079, 1559
BseGI GGATG 2 cut(s) 10, 346
BseLI CCNNNNNNNGG 3 cut(s) 804, 1574, 1592
BseMII CTCAG 1 cut(s) 909
BseNI ACTGG 1 cut(s) 851
BseRI GAGGAG 3 cut(s) 540, 1150, 1332
BseSI GKGCMC 1 cut(s) 459
BseXI GCAGC 7 cut(s) 386, 488, 664, 667, 667, 670, 1204
BshFI GGCC 1 cut(s) 1065
BshNI GGYRCC 1 cut(s) 580
BsiHKAI GWGCWC 2 cut(s) 33, 336
BsiSI CCGG 2 cut(s) 1568, 1587
BslFI GGGAC 3 cut(s) 995, 1039, 1097
BslI CCNNNNNNNGG 3 cut(s) 804, 1574, 1592
BsmAI GTCTC 5 cut(s) 134, 191, 210, 721, 914
BsmFI GGGAC 3 cut(s) 995, 1039, 1097
BsmI GAATGC 1 cut(s) 933
BsnI GGCC 1 cut(s) 1065
Bso31I GGTCTC 3 cut(s) 191, 210, 721
Bsp1286I GDGCHC 4 cut(s) 33, 336, 377, 459
Bsp143I GATC 9 cut(s) 72, 97, 302, 742, 777, 1296, 1349, 1466, 1471
BspACI CCGC 2 cut(s) 393, 1422
BspANI GGCC 1 cut(s) 1065
BspCNI CTCAG 1 cut(s) 910
BspHI TCATGA 2 cut(s) 189, 606
BspLI GGNNCC 2 cut(s) 544, 582
BspMAI CTGCAG 2 cut(s) 506, 1194
BspOI GCTAGC 2 cut(s) 128, 501
BspPI GGATC 3 cut(s) 1357, 1474, 1479
BspQI GCTCTTC 2 cut(s) 262, 622
BspT107I GGYRCC 1 cut(s) 580
BspTNI GGTCTC 3 cut(s) 191, 210, 721
BsrBI CCGCTC 1 cut(s) 1424
BsrFI RCCGGY 1 cut(s) 1567
BsrI ACTGG 1 cut(s) 851
BssAI RCCGGY 1 cut(s) 1567
BssECI CCNNGG 4 cut(s) 209, 250, 1079, 1559
BssMI GATC 9 cut(s) 72, 97, 302, 742, 777, 1296, 1349, 1466, 1471
BssNI GRCGYC 1 cut(s) 1203
BssT1I CCWWGG 2 cut(s) 1079, 1559
Bst4CI ACNGT 8 cut(s) 104, 197, 251, 413, 518, 803, 925, 1053
Bst6I CTCTTC 3 cut(s) 262, 622, 1146
BstACI GRCGYC 1 cut(s) 1203
BstC8I GCNNGC 5 cut(s) 126, 384, 499, 1397, 1422
BstDEI CTNAG 4 cut(s) 49, 240, 566, 918
BstDSI CCRYGG 2 cut(s) 209, 250
BstEII GGTNACC 1 cut(s) 1563
BstF5I GGATG 2 cut(s) 10, 346
BstKTI GATC 9 cut(s) 75, 100, 305, 745, 780, 1299, 1352, 1469, 1474
BstMAI GTCTC 5 cut(s) 134, 191, 210, 721, 914
BstMBI GATC 9 cut(s) 72, 97, 302, 742, 777, 1296, 1349, 1466, 1471
BstMWI GCNNNNNNNGC 6 cut(s) 399, 549, 683, 764, 1421, 1451
BstNSI RCATGY 1 cut(s) 386
BstPI GGTNACC 1 cut(s) 1563
BstSCI CCNGG 1 cut(s) 1585
BstSFI CTRYAG 2 cut(s) 502, 1190
BstSLI GKGCMC 1 cut(s) 459
BstV1I GCAGC 7 cut(s) 386, 488, 664, 667, 667, 670, 1204
BstV2I GAAGAC 1 cut(s) 74
BstX2I RGATCY 4 cut(s) 302, 777, 1349, 1471
BstXI CCANNNNNNTGG 1 cut(s) 521
BstYI RGATCY 4 cut(s) 302, 777, 1349, 1471
BsuI GTATCC 1 cut(s) 861
BsuRI GGCC 1 cut(s) 1065
BtgI CCRYGG 2 cut(s) 209, 250
BtgZI GCGATG 1 cut(s) 1401
BtsCI GGATG 2 cut(s) 10, 346
BtsIMutI CAGTG 1 cut(s) 523
Cac8I GCNNGC 5 cut(s) 126, 384, 499, 1397, 1422
CciI TCATGA 2 cut(s) 189, 606
Cfr10I RCCGGY 1 cut(s) 1567
Cfr13I GGNCC 3 cut(s) 134, 848, 1598
Csp6I GTAC 4 cut(s) 360, 581, 840, 913
CspCI CAANNNNNGTGG 2 cut(s) 1269, 1304
CviAII CATG 5 cut(s) 76, 190, 337, 383, 607
CviQI GTAC 4 cut(s) 360, 581, 840, 913
DdeI CTNAG 4 cut(s) 49, 240, 566, 918
DpnI GATC 9 cut(s) 74, 99, 304, 744, 779, 1298, 1351, 1468, 1473
DpnII GATC 9 cut(s) 72, 97, 302, 742, 777, 1296, 1349, 1466, 1471
DraIII CACNNNGTG 1 cut(s) 961
DrdI GACNNNNNNGTC 1 cut(s) 1605
DseDI GACNNNNNNGTC 1 cut(s) 1605
Eam1104I CTCTTC 3 cut(s) 262, 622, 1146
EarI CTCTTC 3 cut(s) 262, 622, 1146
Ecl136II GAGCTC 1 cut(s) 334
Eco130I CCWWGG 2 cut(s) 1079, 1559
Eco147I AGGCCT 1 cut(s) 1065
Eco24I GRGCYC 2 cut(s) 336, 377
Eco31I GGTCTC 3 cut(s) 191, 210, 721
Eco47I GGWCC 3 cut(s) 134, 848, 1598
Eco53kI GAGCTC 1 cut(s) 334
Eco57I CTGAAG 2 cut(s) 820, 1151
Eco91I GGTNACC 1 cut(s) 1563
EcoICRI GAGCTC 1 cut(s) 334
EcoO65I GGTNACC 1 cut(s) 1563
EcoT14I CCWWGG 2 cut(s) 1079, 1559
EcoT38I GRGCYC 2 cut(s) 336, 377
ErhI CCWWGG 2 cut(s) 1079, 1559
FaeI CATG 5 cut(s) 79, 193, 340, 386, 610
FalI AAGNNNNNCTT 4 cut(s) 112, 144, 1143, 1175
FaqI GGGAC 3 cut(s) 995, 1039, 1097
FatI CATG 5 cut(s) 75, 189, 336, 382, 606
FauI CCCGC 1 cut(s) 1429
FbaI TGATCA 1 cut(s) 1296
Fnu4HI GCNGC 7 cut(s) 400, 502, 656, 678, 681, 684, 1193
FokI GGATG 2 cut(s) 17, 353
FriOI GRGCYC 2 cut(s) 336, 377
Fsp4HI GCNGC 7 cut(s) 400, 502, 656, 678, 681, 684, 1193
FspBI CTAG 9 cut(s) 21, 36, 125, 309, 474, 498, 1074, 1080, 1091
GluI GCNGC 7 cut(s) 400, 502, 656, 678, 681, 684, 1193
GsuI CTGGAG 1 cut(s) 337
HaeIII GGCC 1 cut(s) 1065
HapII CCGG 2 cut(s) 1568, 1587
Hin1I GRCGYC 1 cut(s) 1203
Hin1II CATG 5 cut(s) 79, 193, 340, 386, 610
HincII GTYRAC 1 cut(s) 649
HindII GTYRAC 1 cut(s) 649
HindIII AAGCTT 2 cut(s) 46, 713
HpaI GTTAAC 1 cut(s) 649
HpaII CCGG 2 cut(s) 1568, 1587
HphI GGTGA 3 cut(s) 116, 440, 590
Hpy166II GTNNAC 5 cut(s) 151, 514, 649, 913, 1598
Hpy188I TCNGA 8 cut(s) 436, 786, 1170, 1251, 1354, 1471, 1492, 1528
Hpy8I GTNNAC 5 cut(s) 151, 514, 649, 913, 1598
HpyAV CCTTC 7 cut(s) 98, 636, 1170, 1340, 1494, 1524, 1543
HpyCH4III ACNGT 8 cut(s) 104, 197, 251, 413, 518, 803, 925, 1053
HpyCH4IV ACGT 2 cut(s) 1086, 1203
HpyCH4V TGCA 7 cut(s) 382, 486, 504, 655, 709, 1192, 1415
HpyF10VI GCNNNNNNNGC 6 cut(s) 399, 549, 683, 764, 1421, 1451
HpyF3I CTNAG 4 cut(s) 49, 240, 566, 918
HpySE526I ACGT 2 cut(s) 1086, 1203
Hsp92I GRCGYC 1 cut(s) 1203
Hsp92II CATG 5 cut(s) 79, 193, 340, 386, 610
KpnI GGTACC 1 cut(s) 584
Ksp22I TGATCA 1 cut(s) 1296
KspAI GTTAAC 1 cut(s) 649
Kzo9I GATC 9 cut(s) 72, 97, 302, 742, 777, 1296, 1349, 1466, 1471
LguI GCTCTTC 2 cut(s) 262, 622
LmnI GCTCC 3 cut(s) 494, 527, 548
Lsp1109I GCAGC 7 cut(s) 386, 488, 664, 667, 667, 670, 1204
LweI GCATC 2 cut(s) 74, 1424
MaeI CTAG 9 cut(s) 21, 36, 125, 309, 474, 498, 1074, 1080, 1091
MaeII ACGT 2 cut(s) 1086, 1203
MaeIII GTNAC 3 cut(s) 901, 1563, 1581
MalI GATC 9 cut(s) 74, 99, 304, 744, 779, 1298, 1351, 1468, 1473
MbiI CCGCTC 1 cut(s) 1424
MboI GATC 9 cut(s) 72, 97, 302, 742, 777, 1296, 1349, 1466, 1471
MflI RGATCY 4 cut(s) 302, 777, 1349, 1471
MhlI GDGCHC 4 cut(s) 33, 336, 377, 459
MluCI AATT 4 cut(s) 704, 829, 1225, 1504
MlyI GAGTC 4 cut(s) 136, 223, 604, 1523
MmeI TCCRAC 3 cut(s) 1243, 1365, 1551
MroXI GAANNNNTTC 4 cut(s) 304, 1155, 1214, 1256
MseI TTAA 3 cut(s) 317, 648, 927
MslI CAYNNNNRTG 2 cut(s) 222, 519
MspI CCGG 2 cut(s) 1568, 1587
MspR9I CCNGG 1 cut(s) 1587
Mva1269I GAATGC 1 cut(s) 933
MwoI GCNNNNNNNGC 6 cut(s) 399, 549, 683, 764, 1421, 1451
NciI CCSGG 1 cut(s) 1587
NdeII GATC 9 cut(s) 72, 97, 302, 742, 777, 1296, 1349, 1466, 1471
NheI GCTAGC 2 cut(s) 124, 497
NlaIII CATG 5 cut(s) 79, 193, 340, 386, 610
NlaIV GGNNCC 2 cut(s) 544, 582
NmuCI GTSAC 1 cut(s) 901
NspI RCATGY 1 cut(s) 386
PaeI GCATGC 1 cut(s) 386
PagI TCATGA 2 cut(s) 189, 606
PceI AGGCCT 1 cut(s) 1065
PciSI GCTCTTC 2 cut(s) 262, 622
PctI GAATGC 1 cut(s) 933
PdmI GAANNNNTTC 4 cut(s) 304, 1155, 1214, 1256
PkrI GCNGC 7 cut(s) 401, 503, 657, 679, 682, 685, 1194
PleI GAGTC 4 cut(s) 136, 222, 604, 1523
PpsI GAGTC 4 cut(s) 136, 222, 604, 1523
PsiI TTATAA 1 cut(s) 1110
Psp124BI GAGCTC 1 cut(s) 336
PspEI GGTNACC 1 cut(s) 1563
PspN4I GGNNCC 2 cut(s) 544, 582
PspPI GGNCC 3 cut(s) 134, 848, 1598
PstI CTGCAG 2 cut(s) 506, 1194
PsuI RGATCY 4 cut(s) 302, 777, 1349, 1471
RsaI GTAC 4 cut(s) 361, 582, 841, 914
RsaNI GTAC 4 cut(s) 360, 581, 840, 913
RseI CAYNNNNRTG 2 cut(s) 222, 519
SacI GAGCTC 1 cut(s) 336
SapI GCTCTTC 2 cut(s) 262, 622
SaqAI TTAA 3 cut(s) 317, 648, 927
SatI GCNGC 7 cut(s) 400, 502, 656, 678, 681, 684, 1193
Sau3AI GATC 9 cut(s) 72, 97, 302, 742, 777, 1296, 1349, 1466, 1471
Sau96I GGNCC 3 cut(s) 134, 848, 1598
ScaI AGTACT 2 cut(s) 361, 841
SchI GAGTC 4 cut(s) 136, 223, 604, 1523
ScrFI CCNGG 1 cut(s) 1587
SduI GDGCHC 4 cut(s) 33, 336, 377, 459
SfaNI GCATC 2 cut(s) 74, 1424
SfcI CTRYAG 2 cut(s) 502, 1190
SinI GGWCC 3 cut(s) 134, 848, 1598
SmiMI CAYNNNNRTG 2 cut(s) 222, 519
SmlI CTYRAG 1 cut(s) 1119
SmoI CTYRAG 1 cut(s) 1119
SphI GCATGC 1 cut(s) 386
Sse9I AATT 4 cut(s) 704, 829, 1225, 1504
SseBI AGGCCT 1 cut(s) 1065
SsiI CCGC 2 cut(s) 393, 1422
SspMI CTAG 9 cut(s) 21, 36, 125, 309, 474, 498, 1074, 1080, 1091
SstI GAGCTC 1 cut(s) 336
StuI AGGCCT 1 cut(s) 1065
StyD4I CCNGG 1 cut(s) 1585
StyI CCWWGG 2 cut(s) 1079, 1559
TaaI ACNGT 8 cut(s) 104, 197, 251, 413, 518, 803, 925, 1053
TaiI ACGT 2 cut(s) 1089, 1206
TaqI TCGA 2 cut(s) 330, 558
TasI AATT 4 cut(s) 704, 829, 1225, 1504
TatI WGTACW 2 cut(s) 359, 839
Tru1I TTAA 3 cut(s) 317, 648, 927
Tru9I TTAA 3 cut(s) 317, 648, 927
TscAI CASTG 1 cut(s) 523
TseFI GTSAC 1 cut(s) 901
TseI GCWGC 7 cut(s) 399, 501, 655, 677, 680, 683, 1192
Tsp45I GTSAC 1 cut(s) 901
TspDTI ATGAA 7 cut(s) 27, 206, 362, 595, 717, 1199, 1382
TspGWI ACGGA 5 cut(s) 198, 507, 1282, 1398, 1479
TspRI CASTG 1 cut(s) 523
VpaK11BI GGWCC 3 cut(s) 134, 848, 1598
XbaI TCTAGA 1 cut(s) 35
XceI RCATGY 1 cut(s) 386
XmaJI CCTAGG 1 cut(s) 1079
XmnI GAANNNNTTC 4 cut(s) 304, 1155, 1214, 1256
XspI CTAG 9 cut(s) 21, 36, 125, 309, 474, 498, 1074, 1080, 1091
ZraI GACGTC 1 cut(s) 1204
ZrmI AGTACT 2 cut(s) 361, 841
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.