AT3G63450

Zinc finger (CCCH-type) family protein RNA recognition motif (RRM)-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0001189
Physical Location & Seq
Reverse (-)
23426556 .. 23429356
2801 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G63450.6

Sequence Viewer

Length: 1215 bp
ATGGATGTGTACGAAGCAACTAGGATCGTGCTCTCTAGAATCCAGAACTTAGACCCAGCTAATGCTTCCAAGATCATAGGTCTTCTCCTCCTTCAGGATCACGGTGAGAAGGAGATGATAAGGTTAGCTTTCGGGCCTCAGAATCTCCTTCATTCTGTCATAGCCAAGGCCAAAAAGGACCTCGAAGAGCGGCTTTCTTTCTTGACTCCGAGAACCGCCGAAGATTTTGAGTCCGATTTAGGGTTCGGATGGGGTCACCGCAAAGAGGTGCTTAGGTCCAACTCTGTTCCGCCGAGACTTGCCAATCAGTTCACCGGTTATCCATTCTCCCCCAAAGGTGTCAACTTGCAACAGAGTGAAGCCCAAAGAGCTGCTGCTTTGATGATGGGCGATGACTTGCACAAATTGGGAATATGGAGGCCAGAGAGGATCGACCTCTCGGCCACGGCCTGCCCGGCGTCTAGACAGATCTATCTGACTTTCCCGGCTGACAGTATCTTCAGGGAAGAAGATGTTTCCGACTACTTCAGTACGTTTGGGCCAGTTCAGGACGTGAGGATACCTTACCAGCAGAAGAGGATGTTTGGGTTCGTGACCTTCATGTACCCAGAGACTGTGAAGAGCATTCTGGCCAAAGGGAACCCTCACTTTGTGTGTCATTCCAGGGTTCTTGTTAAGCCTTACAAGGAGAAAGGCAAAGTCCCAGACAAGACTAAGCCATCCAGGGACATTATGGATTTCCAGCTTGGGGGGAGGGCTTTTCATGAAGATCTGCTGTGGAAAAGGAGGTTTGAAGAACGAGCTCTTGAGCTGCAGAGCACAAGGCTGATGAATCTGCAGCTTCTCGACGTCGAGAAACAGTTTCAACTCAATTTCGACCAAACACTACTTGTTTCTCCACGACTAGTTTCAAACAATCAGAGAGTGTGCACAAAAGAGAATGATGAGGATACAATCAAACTTCCAGAGAGCTTGGAGGATGATAGGCTGGTGGATAGCCCAATTGTATCACCGAAACACCATTTTCTCGAGTGTGGGGTAGCTGCAGAAACAAAGGGTTCAGGCTTGTCGTCGCCATCTTTTGATCAGGATGAGTCTAGTACAGGTACATTGAAAGAGCCTCTCAAGTCCTTGAAGTGCCAAATGCCGAGGTTATCAATGATGGGGATAACACAGGGCAGCAGCGGACCAACAACGTGTCGCGTTGGGATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1 Torus zf-CCCH

Protein Analysis

404

Amino Acids

45.67

Weight (kDa)

7.12

Isoelectric Point (pI)

46.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 60 - 105 4.7e-07 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 852
AccBSI CCGCTC 1 cut(s) 190
AccII CGCG 1 cut(s) 1203
AciI CCGC 5 cut(s) 190, 216, 259, 290, 1185
AclWI GGATC 3 cut(s) 32, 105, 437
AcoI YGGCCR 2 cut(s) 441, 630
AcuI CTGAAG 3 cut(s) 77, 484, 511
AcyI GRCGYC 2 cut(s) 458, 849
AdeI CACNNNGTG 1 cut(s) 652
AfaI GTAC 5 cut(s) 11, 532, 605, 1102, 1108
AfiI CCNNNNNNNGG 4 cut(s) 94, 240, 265, 748
AflIII ACRYGT 1 cut(s) 1196
AgeI ACCGGT 1 cut(s) 314
AgsI TTSAA 5 cut(s) 794, 866, 912, 1114, 1135
AhlI ACTAGT 1 cut(s) 904
AjiI CACGTC 1 cut(s) 553
AjnI CCWGG 2 cut(s) 662, 722
AluBI AGCT 9 cut(s) 59, 128, 371, 745, 803, 811, 841, 972, 1043
AluI AGCT 9 cut(s) 59, 128, 371, 745, 803, 811, 841, 972, 1043
Alw21I GWGCWC 4 cut(s) 33, 805, 821, 932
Alw26I GTCTC 2 cut(s) 289, 605
Alw44I GTGCAC 1 cut(s) 928
AlwI GGATC 3 cut(s) 32, 105, 437
AlwNI CAGNNNCTG 1 cut(s) 614
Ama87I CYCGRG 1 cut(s) 1028
AoxI GGCC 7 cut(s) 134, 168, 419, 441, 447, 539, 630
ApaLI GTGCAC 1 cut(s) 928
ApeKI GCWGC 7 cut(s) 371, 374, 811, 838, 1043, 1179, 1182
AsiGI ACCGGT 1 cut(s) 314
AspS9I GGNCC 5 cut(s) 134, 178, 276, 539, 1187
AsuC2I CCSGG 2 cut(s) 455, 485
AsuHPI GGTGA 4 cut(s) 116, 248, 304, 1002
AvaI CYCGRG 1 cut(s) 1028
AvaII GGWCC 3 cut(s) 178, 276, 1187
BaeGI GKGCMC 1 cut(s) 932
BalI TGGCCA 1 cut(s) 632
BanII GRGCYC 1 cut(s) 805
BbsI GAAGAC 1 cut(s) 74
Bbv12I GWGCWC 4 cut(s) 33, 805, 821, 932
BbvI GCAGC 7 cut(s) 358, 361, 798, 850, 1030, 1191, 1194
BccI CCATC 5 cut(s) 243, 379, 727, 1084, 1156
BceAI ACGGC 1 cut(s) 462
BciT130I CCWGG 2 cut(s) 664, 724
BciVI GTATCC 2 cut(s) 552, 943
BclI TGATCA 1 cut(s) 1084
BcnI CCSGG 2 cut(s) 455, 485
BcoDI GTCTC 2 cut(s) 289, 605
BcuI ACTAGT 1 cut(s) 904
BfaI CTAG 5 cut(s) 21, 36, 462, 905, 1098
BfmI CTRYAG 3 cut(s) 812, 836, 1044
BfuI GTATCC 2 cut(s) 552, 943
BglI GCCNNNNNGGC 1 cut(s) 455
BglII AGATCT 2 cut(s) 468, 769
BisI GCNGC 8 cut(s) 191, 372, 375, 812, 839, 1044, 1180, 1183
BlsI GCNGC 8 cut(s) 192, 373, 376, 813, 840, 1045, 1181, 1184
Bme1390I CCNGG 4 cut(s) 455, 485, 664, 724
Bme18I GGWCC 3 cut(s) 178, 276, 1187
BmeT110I CYCGRG 1 cut(s) 1028
BmgBI CACGTC 1 cut(s) 553
BmgT120I GGNCC 5 cut(s) 134, 178, 276, 539, 1187
BmiI GGNNCC 1 cut(s) 641
BmrFI CCNGG 4 cut(s) 455, 485, 664, 724
BpiI GAAGAC 1 cut(s) 74
Bpu10I CCTNAGC 1 cut(s) 272
BpuEI CTTGAG 2 cut(s) 827, 1109
BpuMI CCSGG 2 cut(s) 455, 485
BsaHI GRCGYC 2 cut(s) 458, 849
BsaJI CCNNGG 5 cut(s) 165, 444, 663, 723, 1148
BsaWI WCCGGW 1 cut(s) 314
Bsc4I CCNNNNNNNGG 4 cut(s) 94, 240, 265, 748
Bse118I RCCGGY 1 cut(s) 314
Bse1I ACTGG 1 cut(s) 542
BseBI CCWGG 2 cut(s) 664, 724
BseDI CCNNGG 5 cut(s) 165, 444, 663, 723, 1148
BseGI GGATG 6 cut(s) 10, 254, 585, 719, 985, 1096
BseLI CCNNNNNNNGG 4 cut(s) 94, 240, 265, 748
BseMII CTCAG 1 cut(s) 152
BseNI ACTGG 1 cut(s) 542
BseRI GAGGAG 1 cut(s) 77
BseSI GKGCMC 1 cut(s) 932
BseXI GCAGC 7 cut(s) 358, 361, 798, 850, 1030, 1191, 1194
BseYI CCCAGC 1 cut(s) 55
Bsh1236I CGCG 1 cut(s) 1203
BshFI GGCC 7 cut(s) 136, 170, 421, 443, 449, 541, 632
BshTI ACCGGT 1 cut(s) 314
BsiHKAI GWGCWC 4 cut(s) 33, 805, 821, 932
BsiHKCI CYCGRG 1 cut(s) 1028
BsiSI CCGG 3 cut(s) 315, 455, 485
BslFI GGGAC 2 cut(s) 686, 740
BslI CCNNNNNNNGG 4 cut(s) 94, 240, 265, 748
BsmAI GTCTC 2 cut(s) 289, 605
BsmFI GGGAC 2 cut(s) 686, 740
BsmI GAATGC 1 cut(s) 624
BsnI GGCC 7 cut(s) 136, 170, 421, 443, 449, 541, 632
BsoBI CYCGRG 1 cut(s) 1028
Bsp1286I GDGCHC 4 cut(s) 33, 805, 821, 932
Bsp143I GATC 8 cut(s) 24, 72, 97, 429, 468, 769, 1084, 1209
BspACI CCGC 5 cut(s) 190, 216, 259, 290, 1185
BspANI GGCC 7 cut(s) 136, 170, 421, 443, 449, 541, 632
BspCNI CTCAG 1 cut(s) 151
BspFNI CGCG 1 cut(s) 1203
BspHI TCATGA 1 cut(s) 763
BspLI GGNNCC 1 cut(s) 641
BspMAI CTGCAG 3 cut(s) 816, 840, 1048
BspPI GGATC 3 cut(s) 32, 105, 437
BspQI GCTCTTC 2 cut(s) 180, 614
BsrBI CCGCTC 1 cut(s) 190
BsrFI RCCGGY 1 cut(s) 314
BsrI ACTGG 1 cut(s) 542
BssAI RCCGGY 1 cut(s) 314
BssECI CCNNGG 5 cut(s) 165, 444, 663, 723, 1148
BssMI GATC 8 cut(s) 24, 72, 97, 429, 468, 769, 1084, 1209
BssNI GRCGYC 2 cut(s) 458, 849
BssT1I CCWWGG 1 cut(s) 165
Bst2UI CCWGG 2 cut(s) 664, 724
Bst4CI ACNGT 4 cut(s) 104, 494, 616, 861
Bst6I CTCTTC 3 cut(s) 180, 569, 614
BstACI GRCGYC 2 cut(s) 458, 849
BstC8I GCNNGC 1 cut(s) 451
BstDEI CTNAG 4 cut(s) 49, 138, 272, 714
BstDSI CCRYGG 1 cut(s) 444
BstEII GGTNACC 1 cut(s) 254
BstENI CCTNNNNNAGG 1 cut(s) 92
BstF5I GGATG 6 cut(s) 10, 254, 585, 719, 985, 1096
BstFNI CGCG 1 cut(s) 1203
BstKTI GATC 8 cut(s) 27, 75, 100, 432, 471, 772, 1087, 1212
BstMAI GTCTC 2 cut(s) 289, 605
BstMBI GATC 8 cut(s) 24, 72, 97, 429, 468, 769, 1084, 1209
BstMWI GCNNNNNNNGC 2 cut(s) 368, 455
BstNI CCWGG 2 cut(s) 664, 724
BstPI GGTNACC 1 cut(s) 254
BstSCI CCNGG 4 cut(s) 453, 483, 662, 722
BstSFI CTRYAG 3 cut(s) 812, 836, 1044
BstSLI GKGCMC 1 cut(s) 932
BstUI CGCG 1 cut(s) 1203
BstV1I GCAGC 7 cut(s) 358, 361, 798, 850, 1030, 1191, 1194
BstV2I GAAGAC 1 cut(s) 74
BstX2I RGATCY 3 cut(s) 468, 769, 1209
BstYI RGATCY 3 cut(s) 468, 769, 1209
BsuI GTATCC 2 cut(s) 552, 943
BsuRI GGCC 7 cut(s) 136, 170, 421, 443, 449, 541, 632
BtgI CCRYGG 1 cut(s) 444
BtgZI GCGATG 1 cut(s) 405
BtrI CACGTC 1 cut(s) 553
BtsCI GGATG 6 cut(s) 10, 254, 585, 719, 985, 1096
Cac8I GCNNGC 1 cut(s) 451
CaiI CAGNNNCTG 1 cut(s) 614
CciI TCATGA 1 cut(s) 763
Cfr10I RCCGGY 1 cut(s) 314
Cfr13I GGNCC 5 cut(s) 134, 178, 276, 539, 1187
CseI GACGC 1 cut(s) 447
Csp6I GTAC 5 cut(s) 10, 531, 604, 1101, 1107
CspAI ACCGGT 1 cut(s) 314
CviAII CATG 2 cut(s) 601, 764
CviQI GTAC 5 cut(s) 10, 531, 604, 1101, 1107
DdeI CTNAG 4 cut(s) 49, 138, 272, 714
DpnI GATC 8 cut(s) 26, 74, 99, 431, 470, 771, 1086, 1211
DpnII GATC 8 cut(s) 24, 72, 97, 429, 468, 769, 1084, 1209
DraIII CACNNNGTG 1 cut(s) 652
EaeI YGGCCR 2 cut(s) 441, 630
Eam1104I CTCTTC 3 cut(s) 180, 569, 614
EarI CTCTTC 3 cut(s) 180, 569, 614
EciI GGCGGA 1 cut(s) 279
Ecl136II GAGCTC 1 cut(s) 803
Eco130I CCWWGG 1 cut(s) 165
Eco24I GRGCYC 1 cut(s) 805
Eco47I GGWCC 3 cut(s) 178, 276, 1187
Eco53kI GAGCTC 1 cut(s) 803
Eco57I CTGAAG 3 cut(s) 77, 484, 511
Eco88I CYCGRG 1 cut(s) 1028
Eco91I GGTNACC 1 cut(s) 254
EcoICRI GAGCTC 1 cut(s) 803
EcoNI CCTNNNNNAGG 1 cut(s) 92
EcoO109I RGGNCCY 1 cut(s) 178
EcoO65I GGTNACC 1 cut(s) 254
EcoRII CCWGG 2 cut(s) 662, 722
EcoT14I CCWWGG 1 cut(s) 165
EcoT38I GRGCYC 1 cut(s) 805
ErhI CCWWGG 1 cut(s) 165
FaeI CATG 2 cut(s) 604, 767
FaiI YATR 6 cut(s) 77, 161, 415, 602, 734, 765
FalI AAGNNNNNCTT 6 cut(s) 112, 144, 177, 209, 255, 287
FaqI GGGAC 2 cut(s) 686, 740
FatI CATG 2 cut(s) 600, 763
FbaI TGATCA 1 cut(s) 1084
Fnu4HI GCNGC 8 cut(s) 191, 372, 375, 812, 839, 1044, 1180, 1183
FokI GGATG 6 cut(s) 17, 261, 592, 706, 992, 1103
FriOI GRGCYC 1 cut(s) 805
Fsp4HI GCNGC 8 cut(s) 191, 372, 375, 812, 839, 1044, 1180, 1183
FspBI CTAG 5 cut(s) 21, 36, 462, 905, 1098
GluI GCNGC 8 cut(s) 191, 372, 375, 812, 839, 1044, 1180, 1183
GsaI CCCAGC 1 cut(s) 59
HaeIII GGCC 7 cut(s) 136, 170, 421, 443, 449, 541, 632
HapII CCGG 3 cut(s) 315, 455, 485
HgaI GACGC 1 cut(s) 447
Hin1I GRCGYC 2 cut(s) 458, 849
Hin1II CATG 2 cut(s) 604, 767
HincII GTYRAC 1 cut(s) 343
HindII GTYRAC 1 cut(s) 343
HinfI GANTC 6 cut(s) 39, 142, 205, 230, 832, 1094
HpaII CCGG 3 cut(s) 315, 455, 485
HphI GGTGA 4 cut(s) 116, 248, 304, 1002
Hpy166II GTNNAC 4 cut(s) 10, 312, 343, 930
Hpy188I TCNGA 7 cut(s) 141, 210, 235, 248, 477, 520, 921
Hpy8I GTNNAC 4 cut(s) 10, 312, 343, 930
Hpy99I CGWCG 3 cut(s) 851, 854, 1075
HpyAV CCTTC 4 cut(s) 101, 103, 158, 607
HpyCH4III ACNGT 4 cut(s) 104, 494, 616, 861
HpyCH4IV ACGT 4 cut(s) 533, 552, 849, 1196
HpyCH4V TGCA 6 cut(s) 349, 400, 814, 838, 930, 1046
HpyF10VI GCNNNNNNNGC 2 cut(s) 368, 455
HpyF3I CTNAG 4 cut(s) 49, 138, 272, 714
HpySE526I ACGT 4 cut(s) 533, 552, 849, 1196
Hsp92I GRCGYC 2 cut(s) 458, 849
Hsp92II CATG 2 cut(s) 604, 767
Ksp22I TGATCA 1 cut(s) 1084
Kzo9I GATC 8 cut(s) 24, 72, 97, 429, 468, 769, 1084, 1209
LguI GCTCTTC 2 cut(s) 180, 614
Lsp1109I GCAGC 7 cut(s) 358, 361, 798, 850, 1030, 1191, 1194
MaeI CTAG 5 cut(s) 21, 36, 462, 905, 1098
MaeII ACGT 4 cut(s) 533, 552, 849, 1196
MaeIII GTNAC 2 cut(s) 254, 592
MalI GATC 8 cut(s) 26, 74, 99, 431, 470, 771, 1086, 1211
MbiI CCGCTC 1 cut(s) 190
MboI GATC 8 cut(s) 24, 72, 97, 429, 468, 769, 1084, 1209
MfeI CAATTG 1 cut(s) 1002
MflI RGATCY 3 cut(s) 468, 769, 1209
MhlI GDGCHC 4 cut(s) 33, 805, 821, 932
MlsI TGGCCA 1 cut(s) 632
MluCI AATT 3 cut(s) 404, 871, 1002
MluNI TGGCCA 1 cut(s) 632
MlyI GAGTC 3 cut(s) 199, 239, 1103
MmeI TCCRAC 2 cut(s) 303, 543
Mox20I TGGCCA 1 cut(s) 632
MscI TGGCCA 1 cut(s) 632
MseI TTAA 1 cut(s) 675
Msp20I TGGCCA 1 cut(s) 632
MspA1I CMGCKG 1 cut(s) 1185
MspI CCGG 3 cut(s) 315, 455, 485
MspR9I CCNGG 4 cut(s) 455, 485, 664, 724
MunI CAATTG 1 cut(s) 1002
Mva1269I GAATGC 1 cut(s) 624
MvaI CCWGG 2 cut(s) 664, 724
MvnI CGCG 1 cut(s) 1203
MwoI GCNNNNNNNGC 2 cut(s) 368, 455
NciI CCSGG 2 cut(s) 455, 485
NdeII GATC 8 cut(s) 24, 72, 97, 429, 468, 769, 1084, 1209
NlaIII CATG 2 cut(s) 604, 767
NlaIV GGNNCC 1 cut(s) 641
NmeAIII GCCGAG 3 cut(s) 318, 419, 1173
NmuCI GTSAC 2 cut(s) 254, 592
PaeR7I CTCGAG 1 cut(s) 1028
PagI TCATGA 1 cut(s) 763
PciSI GCTCTTC 2 cut(s) 180, 614
PctI GAATGC 1 cut(s) 624
PfeI GAWTC 3 cut(s) 39, 142, 832
PinAI ACCGGT 1 cut(s) 314
PkrI GCNGC 8 cut(s) 192, 373, 376, 813, 840, 1045, 1181, 1184
PleI GAGTC 3 cut(s) 199, 238, 1102
PpsI GAGTC 3 cut(s) 199, 238, 1102
PpuMI RGGWCCY 1 cut(s) 178
Psp124BI GAGCTC 1 cut(s) 805
Psp5II RGGWCCY 1 cut(s) 178
Psp6I CCWGG 2 cut(s) 662, 722
PspEI GGTNACC 1 cut(s) 254
PspFI CCCAGC 1 cut(s) 55
PspGI CCWGG 2 cut(s) 662, 722
PspN4I GGNNCC 1 cut(s) 641
PspPI GGNCC 5 cut(s) 134, 178, 276, 539, 1187
PspPPI RGGWCCY 1 cut(s) 178
PstI CTGCAG 3 cut(s) 816, 840, 1048
PstNI CAGNNNCTG 1 cut(s) 614
PsuI RGATCY 3 cut(s) 468, 769, 1209
RsaI GTAC 5 cut(s) 11, 532, 605, 1102, 1108
RsaNI GTAC 5 cut(s) 10, 531, 604, 1101, 1107
SacI GAGCTC 1 cut(s) 805
SapI GCTCTTC 2 cut(s) 180, 614
SaqAI TTAA 1 cut(s) 675
SatI GCNGC 8 cut(s) 191, 372, 375, 812, 839, 1044, 1180, 1183
Sau3AI GATC 8 cut(s) 24, 72, 97, 429, 468, 769, 1084, 1209
Sau96I GGNCC 5 cut(s) 134, 178, 276, 539, 1187
SchI GAGTC 3 cut(s) 199, 239, 1103
ScrFI CCNGG 4 cut(s) 455, 485, 664, 724
SduI GDGCHC 4 cut(s) 33, 805, 821, 932
SfcI CTRYAG 3 cut(s) 812, 836, 1044
Sfr274I CTCGAG 1 cut(s) 1028
SinI GGWCC 3 cut(s) 178, 276, 1187
SlaI CTCGAG 1 cut(s) 1028
SmlI CTYRAG 3 cut(s) 806, 1028, 1124
SmoI CTYRAG 3 cut(s) 806, 1028, 1124
SpeI ACTAGT 1 cut(s) 904
Sse9I AATT 3 cut(s) 404, 871, 1002
SsiI CCGC 5 cut(s) 190, 216, 259, 290, 1185
SspMI CTAG 5 cut(s) 21, 36, 462, 905, 1098
SstI GAGCTC 1 cut(s) 805
StyD4I CCNGG 4 cut(s) 453, 483, 662, 722
StyI CCWWGG 1 cut(s) 165
TaaI ACNGT 4 cut(s) 104, 494, 616, 861
TaiI ACGT 4 cut(s) 536, 555, 852, 1199
TaqI TCGA 6 cut(s) 183, 432, 846, 852, 876, 1029
TasI AATT 3 cut(s) 404, 871, 1002
TatI WGTACW 1 cut(s) 1100
TauI GCSGC 1 cut(s) 193
TfiI GAWTC 3 cut(s) 39, 142, 832
Tru1I TTAA 1 cut(s) 675
Tru9I TTAA 1 cut(s) 675
TseFI GTSAC 2 cut(s) 254, 592
TseI GCWGC 7 cut(s) 371, 374, 811, 838, 1043, 1179, 1182
Tsp45I GTSAC 2 cut(s) 254, 592
TspDTI ATGAA 5 cut(s) 140, 589, 752, 780, 845
VneI GTGCAC 1 cut(s) 928
VpaK11BI GGWCC 3 cut(s) 178, 276, 1187
XagI CCTNNNNNAGG 1 cut(s) 92
XbaI TCTAGA 2 cut(s) 35, 461
XcmI CCANNNNNNNNNTGG 1 cut(s) 730
XhoI CTCGAG 1 cut(s) 1028
XspI CTAG 5 cut(s) 21, 36, 462, 905, 1098
ZraI GACGTC 1 cut(s) 850
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.