pycom07g01970

zinc finger CCCH domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0001189
Physical Location & Seq
Forward (+)
1572086 .. 1574978
2893 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g01970.7

Sequence Viewer

Length: 2142 bp
ATGGATTCCTACGAAGCTACAAGAATTGTGTTCTCAAGGATCCAAAATTTGGACCCAGAAAATGCTTCAAAAATCATGGGTCTACTTCTGATACAAGACCATGGGGAGAAGGAAATGATAAGGCTTGCTTTTGGGCCTGAAGCTTTTCTCCACTCTGTCATTCTCAAGGCAAGGAAGGAGCTGGGAGTGCCCTTATCATCGAACTCTCCTACTCCTTCCACCCCTCCTTCTCCTTCCCCCTTCACAAACCCCATCGCTATCTCCAGGCAGAACTCTTGCTCTTCCGCCTCGAGGCTCTCTCTCACTATCCCAAACCCTTCTTCCTCCGCTTCCTGGGCTGCTCTCTCCGAGCTCAGAAACCAAGAAGAAATAAACAGCTTTTATGGAGGAGTTCCAACTGATGTGATGGATGAGTTTCAGCTCCAAGAGCAGCTCTCCTTCCTTAACGACGGCTGTCCACCACCAATGGTGGGACCCAAGTCGCCTGATTTTTTCTACCCTCAGTCCGACTTGTCTTCCAGTCCGAGTCCCACCACCGCTTCTGATCCGAACCTTTTTCCCTCCTTCCACGGCTCTGCTAATTGGGGTGGTGGTGGGCCGCTCCACCGACGAAGTTGCTCAGTCAGCGACGTGAGCTTGGAGGACCCCAACTCAGGGTTTGGGTGGAAACCCTGCTTGTATTTCGCCAGAGGCTACTGCAAGAACGGAGCCAGCTGCAGATTCTTGCACGGCGGCGGGCTCGGAGACGCCGTCGTCGACGGCGGTGGACAGGTGGTTGTTGGGTCGCCGAGCAAGCTTGAGATGACGGACCACGAAGCACTTCTTAGATCTAAAACCGCTCAGCAGCAGAGACTTGCTGCTGCTTCTCAGCTCATGGCGGCGTCAGCCAACTCCTTCCCGTACTCCTCGAAGTGCATGAACTTCCTTCTTCAGCAGCAACAAACTGACGCCCAAAGGGCTGCAGCAGCTGCCTTGATGATGGGTGATGATATGCACAAATTCAGCCGATCCCGCCTCGAAAGATCTGATTTTTCGATGAATGGCGGGGCGGGGATTGTGAACCCAGCTTCAAGGCAGATATACCTGACTTTCCCGGCCGATAGCACTTTCAGAGAGGAAGATGTCTCAAACTATTTCAGCATGTACGGGCCGGTTCAAGACGTGCGAATCCCGTACCAGCAGAAGAGGATGTTTGGATTCGTTACATTCATTTACCCGGAGACGGTGAAGCTCATCTTGGCCAAAGGGAACCCTCATTTTGTTTGTGATGCAAGAGTGCTTGTCAAGCCTTACAAGGAGAAGGGCAAAGTTCATGAAAAGAAACAACAGCAACAAGGGGAAAGGGGAGATTTGTCCCCCTGTGGTACACCTACTGGGCTGGATGCTAGAGACCCTTATGATCTCCAACTAGGACCGCGGATGTTTTACAATCCTCAAGATATGCTGTGGAGGAGGAAGCTGGAGGAGCAAGCTGAGTTGCAGCAAGCACTTGAAATTCAAAGTCGAAGACTGATGGGCCTGCAGCTACTTGATGTCAAGAAGCATCACCACTGTGCGCTTTCTGCCAGCAGTCCGATTCACTCCCCTACCCAGTCTCCCAACATGTTCAATCAAAACCTGATGCATCCTTCGGTTCACAGCGGCCAAGAAGTTCCCCACGATAACTGTTCTTCTCTGATGCCACACATGACTCCAGCAGTGGATGCCGATCAGCTGCTTAAGGAAGCAGCTGCCGCTGTTCTAGGTGAAGGTTTAATTGCCAACAATGACGAAAATGGCAGTGGTAGCCGAAGTGGCAATAGTAGTGGCAAGGAGACCCCCCATGATGAGATTGGTGACTTGCTCGAAGGCTTGGAGCACAATCTTCCCGATAGTCCTTTCGCATCTCCAACAAAGCCCGCTGGAGAGTACACATTGTCCGCCTTCCCCAATGGGACTAGCGAGGCCAATGGTTCGGATGCCCCAGCTGCAACCGCTAATATTAACGTGGCAAATTCCCTTCTTCCAGCGTCTTCCACCCTGGACGTGGCATCCTTCAAGTCTTTCAACTGCCAAATTCCACGGTTGAACTCCGGCCACGGAGCCATAGGGATGTACGCCGGCACAGGAGCGGGGCCAAAATGCCCGGTTGGAACTTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1 Torus zf-CCCH

Protein Analysis

714

Amino Acids

76.97

Weight (kDa)

5.87

Isoelectric Point (pI)

55.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 752
AbsI CCTCGAGG 1 cut(s) 289
AccB7I CCANNNNNTGG 1 cut(s) 49
AccBSI CCGCTC 3 cut(s) 601, 839, 2111
AccI GTMKAC 2 cut(s) 82, 756
AccII CGCG 1 cut(s) 1417
AclWI GGATC 4 cut(s) 34, 47, 539, 1002
AcoI YGGCCR 4 cut(s) 1095, 1239, 1642, 2074
AcsI RAATTY 5 cut(s) 46, 998, 1494, 1993, 2055
AcuI CTGAAG 2 cut(s) 159, 914
AcyI GRCGYC 3 cut(s) 747, 881, 948
AfaI GTAC 6 cut(s) 902, 1145, 1175, 1366, 1910, 2096
AfiI CCNNNNNNNGG 8 cut(s) 49, 291, 333, 470, 653, 654, 1222, 2026
AflII CTTAAG 1 cut(s) 1718
AflIII ACRYGT 1 cut(s) 1602
AgsI TTSAA 9 cut(s) 69, 1071, 1157, 1493, 1499, 1609, 2038, 2047, 2068
AjiI CACGTC 3 cut(s) 631, 1162, 2026
AjnI CCWGG 3 cut(s) 263, 332, 2019
AjuI GAANNNNNNNTTGG 4 cut(s) 304, 336, 1220, 1252
AleI CACNNNNGTG 1 cut(s) 1551
Alw21I GWGCWC 2 cut(s) 354, 1860
Alw26I GTCTC 7 cut(s) 738, 844, 1129, 1214, 1383, 1599, 1808
AlwI GGATC 4 cut(s) 34, 47, 539, 1002
AlwNI CAGNNNCTG 1 cut(s) 968
Ama87I CYCGRG 1 cut(s) 289
ApoI RAATTY 5 cut(s) 46, 998, 1494, 1993, 2055
Asp700I GAANNNNTTC 2 cut(s) 144, 819
AspLEI GCGC 1 cut(s) 1558
AsuC2I CCSGG 3 cut(s) 1094, 1217, 2126
AsuHPI GGTGA 5 cut(s) 995, 1237, 1538, 1757, 1847
AvaI CYCGRG 1 cut(s) 289
AvaII GGWCC 5 cut(s) 52, 473, 643, 808, 1412
BaeGI GKGCMC 1 cut(s) 192
BalI TGGCCA 1 cut(s) 1241
BamHI GGATCC 1 cut(s) 39
BanII GRGCYC 2 cut(s) 354, 741
BbsI GAAGAC 3 cut(s) 507, 1513, 2004
Bbv12I GWGCWC 2 cut(s) 354, 1860
BccI CCATC 4 cut(s) 260, 400, 973, 1507
BceAI ACGGC 5 cut(s) 466, 586, 734, 745, 775
BcgI CGANNNNNNTGC 2 cut(s) 597, 631
BciT130I CCWGG 3 cut(s) 265, 334, 2021
BcnI CCSGG 3 cut(s) 1094, 1217, 2126
BcoDI GTCTC 7 cut(s) 738, 844, 1129, 1214, 1383, 1599, 1808
BfaI CTAG 4 cut(s) 1386, 1409, 1742, 1938
BfmI CTRYAG 3 cut(s) 715, 960, 1520
BfrI CTTAAG 1 cut(s) 1718
BglI GCCNNNNNGGC 2 cut(s) 956, 1794
BglII AGATCT 2 cut(s) 827, 1022
BlpI GCTNAGC 1 cut(s) 840
Bme1390I CCNGG 6 cut(s) 265, 334, 1094, 1217, 2021, 2126
Bme18I GGWCC 5 cut(s) 52, 473, 643, 808, 1412
BmeT110I CYCGRG 1 cut(s) 289
BmgBI CACGTC 3 cut(s) 631, 1162, 2026
BmiI GGNNCC 9 cut(s) 41, 54, 474, 475, 645, 709, 1250, 2083, 2115
BmrFI CCNGG 6 cut(s) 265, 334, 1094, 1217, 2021, 2126
BmrI ACTGGG 2 cut(s) 1383, 1585
BmuI ACTGGG 2 cut(s) 1383, 1585
BoxI GACNNNNGTC 2 cut(s) 453, 478
BpiI GAAGAC 3 cut(s) 507, 1513, 2004
BplI GAGNNNNNCTC 4 cut(s) 283, 315, 419, 451
BpmI CTGGAG 4 cut(s) 247, 1481, 1677, 1923
Bpu1102I GCTNAGC 1 cut(s) 840
BpuEI CTTGAG 4 cut(s) 19, 149, 818, 1419
BpuMI CCSGG 3 cut(s) 1094, 1217, 2126
BsaBI GATNNNNATC 1 cut(s) 1707
BsaHI GRCGYC 3 cut(s) 747, 881, 948
BsaI GGTCTC 2 cut(s) 1383, 1808
BsaJI CCNNGG 7 cut(s) 100, 333, 568, 1415, 2019, 2060, 2077
BsaXI ACNNNNNCTCC 4 cut(s) 735, 765, 1579, 1609
Bsc4I CCNNNNNNNGG 8 cut(s) 49, 291, 333, 470, 653, 654, 1222, 2026
Bse118I RCCGGY 2 cut(s) 1150, 2099
Bse1I ACTGG 3 cut(s) 519, 1378, 1591
Bse8I GATNNNNATC 1 cut(s) 1707
BseBI CCWGG 3 cut(s) 265, 334, 2021
BseDI CCNNGG 7 cut(s) 100, 333, 568, 1415, 2019, 2060, 2077
BseGI GGATG 9 cut(s) 415, 1194, 1387, 1425, 1624, 1708, 1963, 2030, 2097
BseJI GATNNNNATC 1 cut(s) 1707
BseLI CCNNNNNNNGG 8 cut(s) 49, 291, 333, 470, 653, 654, 1222, 2026
BseMII CTCAG 7 cut(s) 367, 515, 633, 666, 854, 881, 1464
BseNI ACTGG 3 cut(s) 519, 1378, 1591
BseRI GAGGAG 4 cut(s) 402, 895, 1465, 1478
BseSI GKGCMC 1 cut(s) 192
BseX3I CGGCCG 1 cut(s) 1095
BseYI CCCAGC 3 cut(s) 181, 1063, 1963
Bsh1236I CGCG 1 cut(s) 1417
Bsh1285I CGRYCG 1 cut(s) 1098
BsiEI CGRYCG 1 cut(s) 1098
BsiHKAI GWGCWC 2 cut(s) 354, 1860
BsiHKCI CYCGRG 1 cut(s) 289
BsiSI CCGG 6 cut(s) 1094, 1151, 1217, 2073, 2100, 2126
BslFI GGGAC 4 cut(s) 486, 513, 1339, 1948
BslI CCNNNNNNNGG 8 cut(s) 49, 291, 333, 470, 653, 654, 1222, 2026
BsmAI GTCTC 7 cut(s) 738, 844, 1129, 1214, 1383, 1599, 1808
BsmBI CGTCTC 2 cut(s) 738, 1214
BsmFI GGGAC 4 cut(s) 486, 513, 1339, 1948
Bso31I GGTCTC 2 cut(s) 1383, 1808
BsoBI CYCGRG 1 cut(s) 289
Bsp1286I GDGCHC 4 cut(s) 192, 354, 741, 1860
Bsp143I GATC 7 cut(s) 39, 544, 827, 1007, 1022, 1399, 1708
Bsp1720I GCTNAGC 1 cut(s) 840
Bsp19I CCATGG 1 cut(s) 100
BspCNI CTCAG 7 cut(s) 366, 514, 632, 665, 853, 880, 1465
BspFNI CGCG 1 cut(s) 1417
BspHI TCATGA 1 cut(s) 1312
BspLI GGNNCC 9 cut(s) 41, 54, 474, 475, 645, 709, 1250, 2083, 2115
BspMAI CTGCAG 3 cut(s) 719, 964, 1524
BspPI GGATC 4 cut(s) 34, 47, 539, 1002
BspQI GCTCTTC 1 cut(s) 286
BspTI CTTAAG 1 cut(s) 1718
BspTNI GGTCTC 2 cut(s) 1383, 1808
BsrBI CCGCTC 3 cut(s) 601, 839, 2111
BsrFI RCCGGY 2 cut(s) 1150, 2099
BsrI ACTGG 3 cut(s) 519, 1378, 1591
BssAI RCCGGY 2 cut(s) 1150, 2099
BssECI CCNNGG 7 cut(s) 100, 333, 568, 1415, 2019, 2060, 2077
BssMI GATC 7 cut(s) 39, 544, 827, 1007, 1022, 1399, 1708
BssNI GRCGYC 3 cut(s) 747, 881, 948
BssT1I CCWWGG 1 cut(s) 100
Bst2UI CCWGG 3 cut(s) 265, 334, 2021
Bst4CI ACNGT 4 cut(s) 1225, 1553, 1667, 2064
Bst6I CTCTTC 2 cut(s) 286, 1178
BstACI GRCGYC 3 cut(s) 747, 881, 948
BstAFI CTTAAG 1 cut(s) 1718
BstAPI GCANNNNNTGC 2 cut(s) 968, 1703
BstDEI CTNAG 8 cut(s) 353, 501, 619, 652, 824, 840, 867, 1473
BstDSI CCRYGG 5 cut(s) 100, 568, 1415, 2060, 2077
BstF5I GGATG 9 cut(s) 415, 1194, 1387, 1425, 1624, 1708, 1963, 2030, 2097
BstFNI CGCG 1 cut(s) 1417
BstHHI GCGC 1 cut(s) 1558
BstKTI GATC 7 cut(s) 42, 547, 830, 1010, 1025, 1402, 1711
BstMAI GTCTC 7 cut(s) 738, 844, 1129, 1214, 1383, 1599, 1808
BstMBI GATC 7 cut(s) 39, 544, 827, 1007, 1022, 1399, 1708
BstMCI CGRYCG 1 cut(s) 1098
BstNI CCWGG 3 cut(s) 265, 334, 2021
BstNSI RCATGY 2 cut(s) 1144, 1606
BstPAI GACNNNNGTC 2 cut(s) 453, 478
BstSCI CCNGG 6 cut(s) 263, 332, 1092, 1215, 2019, 2124
BstSFI CTRYAG 3 cut(s) 715, 960, 1520
BstSLI GKGCMC 1 cut(s) 192
BstUI CGCG 1 cut(s) 1417
BstV2I GAAGAC 3 cut(s) 507, 1513, 2004
BstX2I RGATCY 3 cut(s) 39, 827, 1022
BstYI RGATCY 3 cut(s) 39, 827, 1022
BstZI CGGCCG 1 cut(s) 1095
BtgI CCRYGG 5 cut(s) 100, 568, 1415, 2060, 2077
BtgZI GCGATG 1 cut(s) 238
BtrI CACGTC 3 cut(s) 631, 1162, 2026
BtsCI GGATG 9 cut(s) 415, 1194, 1387, 1425, 1624, 1708, 1963, 2030, 2097
BtsI GCAGTG 2 cut(s) 1704, 1786
BtsIMutI CAGTG 3 cut(s) 1549, 1704, 1786
CaiI CAGNNNCTG 1 cut(s) 968
CciI TCATGA 1 cut(s) 1312
CfoI GCGC 1 cut(s) 1558
Cfr10I RCCGGY 2 cut(s) 1150, 2099
Cfr42I CCGCGG 1 cut(s) 1418
CseI GACGC 4 cut(s) 755, 870, 956, 1998
Csp6I GTAC 6 cut(s) 901, 1144, 1174, 1365, 1909, 2095
CspCI CAANNNNNGTGG 2 cut(s) 1786, 1821
CviAII CATG 9 cut(s) 76, 101, 874, 916, 1141, 1313, 1603, 1687, 1823
CviQI GTAC 6 cut(s) 901, 1144, 1174, 1365, 1909, 2095
DdeI CTNAG 8 cut(s) 353, 501, 619, 652, 824, 840, 867, 1473
DpnI GATC 7 cut(s) 41, 546, 829, 1009, 1024, 1401, 1710
DpnII GATC 7 cut(s) 39, 544, 827, 1007, 1022, 1399, 1708
DrdI GACNNNNNNGTC 1 cut(s) 752
DseDI GACNNNNNNGTC 1 cut(s) 752
EaeI YGGCCR 4 cut(s) 1095, 1239, 1642, 2074
EagI CGGCCG 1 cut(s) 1095
Eam1104I CTCTTC 2 cut(s) 286, 1178
EarI CTCTTC 2 cut(s) 286, 1178
EciI GGCGGA 2 cut(s) 274, 1909
Ecl136II GAGCTC 1 cut(s) 352
EclXI CGGCCG 1 cut(s) 1095
Eco130I CCWWGG 1 cut(s) 100
Eco24I GRGCYC 2 cut(s) 354, 741
Eco31I GGTCTC 2 cut(s) 1383, 1808
Eco47I GGWCC 5 cut(s) 52, 473, 643, 808, 1412
Eco52I CGGCCG 1 cut(s) 1095
Eco53kI GAGCTC 1 cut(s) 352
Eco57I CTGAAG 2 cut(s) 159, 914
Eco88I CYCGRG 1 cut(s) 289
EcoICRI GAGCTC 1 cut(s) 352
EcoO109I RGGNCCY 2 cut(s) 473, 643
EcoRII CCWGG 3 cut(s) 263, 332, 2019
EcoT14I CCWWGG 1 cut(s) 100
EcoT22I ATGCAT 1 cut(s) 1626
EcoT38I GRGCYC 2 cut(s) 354, 741
ErhI CCWWGG 1 cut(s) 100
Esp3I CGTCTC 2 cut(s) 738, 1214
FaeI CATG 9 cut(s) 79, 104, 877, 919, 1144, 1316, 1606, 1690, 1826
FalI AAGNNNNNCTT 6 cut(s) 112, 144, 807, 839, 1220, 1252
FaqI GGGAC 4 cut(s) 486, 513, 1339, 1948
FatI CATG 9 cut(s) 75, 100, 873, 915, 1140, 1312, 1602, 1686, 1822
FauI CCCGC 6 cut(s) 728, 1019, 1037, 1042, 1906, 2104
FblI GTMKAC 2 cut(s) 82, 756
FokI GGATG 9 cut(s) 422, 1201, 1394, 1432, 1611, 1715, 1970, 2017, 2104
FriOI GRGCYC 2 cut(s) 354, 741
FspBI CTAG 4 cut(s) 1386, 1409, 1742, 1938
GlaI GCGC 1 cut(s) 1557
GsaI CCCAGC 3 cut(s) 185, 1067, 1967
GsuI CTGGAG 4 cut(s) 247, 1481, 1677, 1923
HapII CCGG 6 cut(s) 1094, 1151, 1217, 2073, 2100, 2126
HgaI GACGC 4 cut(s) 755, 870, 956, 1998
HhaI GCGC 1 cut(s) 1558
Hin1I GRCGYC 3 cut(s) 747, 881, 948
Hin1II CATG 9 cut(s) 79, 104, 877, 919, 1144, 1316, 1606, 1690, 1826
Hin6I GCGC 1 cut(s) 1556
HinP1I GCGC 1 cut(s) 1556
HincII GTYRAC 1 cut(s) 757
HindII GTYRAC 1 cut(s) 757
HindIII AAGCTT 2 cut(s) 141, 794
HinfI GANTC 7 cut(s) 5, 526, 720, 1167, 1197, 1576, 1690
HpaII CCGG 6 cut(s) 1094, 1151, 1217, 2073, 2100, 2126
HphI GGTGA 5 cut(s) 995, 1237, 1538, 1757, 1847
Hpy166II GTNNAC 8 cut(s) 83, 458, 757, 767, 1060, 1367, 1636, 1911
Hpy188III TCNNGA 5 cut(s) 1157, 1313, 1436, 1537, 1868
Hpy8I GTNNAC 8 cut(s) 83, 458, 757, 767, 1060, 1367, 1636, 1911
Hpy99I CGWCG 6 cut(s) 452, 612, 632, 755, 758, 761
HpyCH4III ACNGT 4 cut(s) 1225, 1553, 1667, 2064
HpyCH4IV ACGT 4 cut(s) 630, 1161, 1986, 2025
HpyF3I CTNAG 8 cut(s) 353, 501, 619, 652, 824, 840, 867, 1473
HpySE526I ACGT 4 cut(s) 630, 1161, 1986, 2025
Hsp92I GRCGYC 3 cut(s) 747, 881, 948
Hsp92II CATG 9 cut(s) 79, 104, 877, 919, 1144, 1316, 1606, 1690, 1826
HspAI GCGC 1 cut(s) 1556
KflI GGGWCCC 1 cut(s) 473
KroI GCCGGC 1 cut(s) 2099
KroNI GCCGGC 1 cut(s) 2101
KspI CCGCGG 1 cut(s) 1418
Kzo9I GATC 7 cut(s) 39, 544, 827, 1007, 1022, 1399, 1708
LguI GCTCTTC 1 cut(s) 286
LmnI GCTCC 8 cut(s) 178, 426, 606, 707, 1465, 1855, 2081, 2108
MaeI CTAG 4 cut(s) 1386, 1409, 1742, 1938
MaeII ACGT 4 cut(s) 630, 1161, 1986, 2025
MaeIII GTNAC 2 cut(s) 1201, 1835
MalI GATC 7 cut(s) 41, 546, 829, 1009, 1024, 1401, 1710
MbiI CCGCTC 3 cut(s) 601, 839, 2111
MboI GATC 7 cut(s) 39, 544, 827, 1007, 1022, 1399, 1708
MflI RGATCY 3 cut(s) 39, 827, 1022
MhlI GDGCHC 4 cut(s) 192, 354, 741, 1860
MlsI TGGCCA 1 cut(s) 1241
MluCI AATT 8 cut(s) 24, 46, 580, 998, 1494, 1755, 1993, 2055
MluNI TGGCCA 1 cut(s) 1241
MlyI GAGTC 2 cut(s) 535, 1684
MmeI TCCRAC 5 cut(s) 419, 531, 1429, 1913, 2110
Mox20I TGGCCA 1 cut(s) 1241
Mph1103I ATGCAT 1 cut(s) 1626
MroNI GCCGGC 1 cut(s) 2099
MroXI GAANNNNTTC 2 cut(s) 144, 819
MscI TGGCCA 1 cut(s) 1241
MseI TTAA 4 cut(s) 444, 1719, 1754, 1983
MslI CAYNNNNRTG 2 cut(s) 1551, 2090
Msp20I TGGCCA 1 cut(s) 1241
MspA1I CMGCKG 9 cut(s) 714, 968, 1417, 1641, 1714, 1730, 1736, 1901, 1967
MspCI CTTAAG 1 cut(s) 1718
MspI CCGG 6 cut(s) 1094, 1151, 1217, 2073, 2100, 2126
MspR9I CCNGG 6 cut(s) 265, 334, 1094, 1217, 2021, 2126
MvaI CCWGG 3 cut(s) 265, 334, 2021
MvnI CGCG 1 cut(s) 1417
NaeI GCCGGC 1 cut(s) 2101
NciI CCSGG 3 cut(s) 1094, 1217, 2126
NcoI CCATGG 1 cut(s) 100
NdeII GATC 7 cut(s) 39, 544, 827, 1007, 1022, 1399, 1708
NgoMIV GCCGGC 1 cut(s) 2099
NlaIII CATG 9 cut(s) 79, 104, 877, 919, 1144, 1316, 1606, 1690, 1826
NlaIV GGNNCC 9 cut(s) 41, 54, 474, 475, 645, 709, 1250, 2083, 2115
NmeAIII GCCGAG 1 cut(s) 813
NmuCI GTSAC 1 cut(s) 1835
NsiI ATGCAT 1 cut(s) 1626
NspI RCATGY 2 cut(s) 1144, 1606
OliI CACNNNNGTG 1 cut(s) 1551
PaeR7I CTCGAG 1 cut(s) 289
PagI TCATGA 1 cut(s) 1312
PciI ACATGT 1 cut(s) 1602
PciSI GCTCTTC 1 cut(s) 286
PcsI WCGNNNNNNNCGW 2 cut(s) 747, 753
PdiI GCCGGC 1 cut(s) 2101
PdmI GAANNNNTTC 2 cut(s) 144, 819
PfeI GAWTC 5 cut(s) 5, 720, 1167, 1197, 1576
PflFI GACNNNGTC 1 cut(s) 749
PflMI CCANNNNNTGG 1 cut(s) 49
PleI GAGTC 2 cut(s) 534, 1684
PpsI GAGTC 2 cut(s) 534, 1684
PpuMI RGGWCCY 2 cut(s) 473, 643
PscI ACATGT 1 cut(s) 1602
PshAI GACNNNNGTC 2 cut(s) 453, 478
Psp124BI GAGCTC 1 cut(s) 354
Psp5II RGGWCCY 2 cut(s) 473, 643
Psp6I CCWGG 3 cut(s) 263, 332, 2019
PspFI CCCAGC 3 cut(s) 181, 1063, 1963
PspGI CCWGG 3 cut(s) 263, 332, 2019
PspN4I GGNNCC 9 cut(s) 41, 54, 474, 475, 645, 709, 1250, 2083, 2115
PspPPI RGGWCCY 2 cut(s) 473, 643
PspXI VCTCGAGB 1 cut(s) 289
PstI CTGCAG 3 cut(s) 719, 964, 1524
PstNI CAGNNNCTG 1 cut(s) 968
PsuI RGATCY 3 cut(s) 39, 827, 1022
PsyI GACNNNGTC 1 cut(s) 749
PvuII CAGCTG 5 cut(s) 714, 968, 1714, 1730, 1967
RsaI GTAC 6 cut(s) 902, 1145, 1175, 1366, 1910, 2096
RsaNI GTAC 6 cut(s) 901, 1144, 1174, 1365, 1909, 2095
RseI CAYNNNNRTG 2 cut(s) 1551, 2090
SacI GAGCTC 1 cut(s) 354
SacII CCGCGG 1 cut(s) 1418
SalI GTCGAC 1 cut(s) 755
SapI GCTCTTC 1 cut(s) 286
SaqAI TTAA 4 cut(s) 444, 1719, 1754, 1983
Sau3AI GATC 7 cut(s) 39, 544, 827, 1007, 1022, 1399, 1708
SchI GAGTC 2 cut(s) 535, 1684
ScrFI CCNGG 6 cut(s) 265, 334, 1094, 1217, 2021, 2126
SduI GDGCHC 4 cut(s) 192, 354, 741, 1860
SfcI CTRYAG 3 cut(s) 715, 960, 1520
Sfr274I CTCGAG 1 cut(s) 289
Sfr303I CCGCGG 1 cut(s) 1418
SgrBI CCGCGG 1 cut(s) 1418
SgrDI CGTCGACG 1 cut(s) 755
SinI GGWCC 5 cut(s) 52, 473, 643, 808, 1412
SlaI CTCGAG 1 cut(s) 289
SmiMI CAYNNNNRTG 2 cut(s) 1551, 2090
SmlI CTYRAG 6 cut(s) 34, 164, 289, 797, 1434, 1718
SmoI CTYRAG 6 cut(s) 34, 164, 289, 797, 1434, 1718
Sse9I AATT 8 cut(s) 24, 46, 580, 998, 1494, 1755, 1993, 2055
SspI AATATT 1 cut(s) 1981
SspMI CTAG 4 cut(s) 1386, 1409, 1742, 1938
SstI GAGCTC 1 cut(s) 354
StyD4I CCNGG 6 cut(s) 263, 332, 1092, 1215, 2019, 2124
StyI CCWWGG 1 cut(s) 100
TaaI ACNGT 4 cut(s) 1225, 1553, 1667, 2064
TaiI ACGT 4 cut(s) 633, 1164, 1989, 2028
TaqI TCGA 8 cut(s) 200, 290, 756, 908, 1017, 1034, 1504, 1845
TasI AATT 8 cut(s) 24, 46, 580, 998, 1494, 1755, 1993, 2055
TatI WGTACW 1 cut(s) 1908
TauI GCSGC 5 cut(s) 601, 735, 881, 1644, 1736
TfiI GAWTC 5 cut(s) 5, 720, 1167, 1197, 1576
Tru1I TTAA 4 cut(s) 444, 1719, 1754, 1983
Tru9I TTAA 4 cut(s) 444, 1719, 1754, 1983
TscAI CASTG 3 cut(s) 1556, 1704, 1786
TseFI GTSAC 1 cut(s) 1835
Tsp45I GTSAC 1 cut(s) 1835
TspDTI ATGAA 5 cut(s) 932, 1052, 1198, 1301, 1329
TspGWI ACGGA 3 cut(s) 720, 821, 2094
TspRI CASTG 3 cut(s) 1556, 1704, 1786
Tth111I GACNNNGTC 1 cut(s) 749
Van91I CCANNNNNTGG 1 cut(s) 49
Vha464I CTTAAG 1 cut(s) 1718
VpaK11BI GGWCC 5 cut(s) 52, 473, 643, 808, 1412
XapI RAATTY 5 cut(s) 46, 998, 1494, 1993, 2055
XceI RCATGY 2 cut(s) 1144, 1606
XcmI CCANNNNNNNNNTGG 2 cut(s) 1829, 2023
XhoI CTCGAG 1 cut(s) 289
XmiI GTMKAC 2 cut(s) 82, 756
XmnI GAANNNNTTC 2 cut(s) 144, 819
XspI CTAG 4 cut(s) 1386, 1409, 1742, 1938
Zsp2I ATGCAT 1 cut(s) 1626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.