MD07G1026000.v1.1

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0015798
Physical Location & Seq
Forward (+)
2199109 .. 2206996
7888 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1026000.v1.1.491

Sequence Viewer

Length: 3126 bp
ATGAGAGAGAAAATGGAGGAATACTTGCGGACGCACTTCGACGTCGAACCCAAACGGCCGTCCGAGGAGGCCCTGAAGCGGTGGAGATCCGCGGTTTCCATTGTCAAGAATCCTACCCGAAGGTTCCGCATGGTCGCTGATCTCGCCAAGCGCGCCGAGGACGAGCGGAAGCGCAAGAACCTCCAGGAAAAGATACGTGTAGCTCTTTACGTACAAAAAGCGGCATTGCAATTCATCGATGCTGGCAATCGTGGGGGATATAATCTTTCCAAAGAAGTAAAAGATGCTGGTTTTGGCATTGATCCTGAAGAAATTGCATCCTTTGCTCGATCTCATGATACGAAGGGTTTGGAAGGACACGGAGGAGTATCAGGATTAGCAGGAGAGGTTTCTGTATCGCTGAAAGATGGTGTTGTTTCAAGCAAGATACCTATTAGGCAGAATATATTTGGCTTAAACCGATATGCAGAGAAACCTTCTAAAGGTTTCTTGATGTTTCTCTGGGAAGCCTTACAAGATTTAACTTTAATTATCCTCATGGTTGCTGCTGCGGTCTCTATAGGTGTTGGAATTGCAACTGAAGGAATGCCGAAAGGTATGTATGATGGCTTGGGAATAATAGTTAGCATATTGTTAGTAGTGTTGGTGACTGCAATTAGCGACTACAAGCAGTCACTGCAATTTAAAGAACTGGACAAGGAGAAGAAAAACATAATGGTTCAGGTGACTAGAGACGGACGTAGACAGAAGGTCTCTATCTACGATTTGGTGGTTGGAGAAATTGTTCATCTTTCAATCGGTGATCAAGTTCCAGCTGATGGTATTTTCATATCAGGCCACAGCTTACAAGTCGACGAATCAAGCTTGTCAGGCGAGAGTGAGCCAGTGGACATAAATCAAGATAGACCTTTTCTTCTTGCAGGAACCAAAGTGCAAGATGGTTCTGGTAAAATGCTGGTGACATCAGTTGGTATGAGGACCGAATGGGGACGACTAATGGTTACTCTGAGTGAAGGGGGAGACAATGAGACACCACTGCAGGTGAAACTTAATGGAGTTGCAACCATTATCGGAAAAATTGGTTTGGCTTTCGCGGTGCTGACATTTATAGTTCTGATTTCAAGGTTTTTAGTAAACAAGGCGGTTCACCATCACATGAAAGACTGGTCTTCCAAAGATGCACAAATGCTTTTGAATTACTTTTCGACTGCCGTCATTATAATTGTTGTTGCAGTCCCAGAAGGGCTACCTCTGGCAGTGACATTAAGTCTTGCCTTTGCAATGAAAAAACTCATGTCGGATAAAGCACTTGTGAGGCATCTCTCTGCATGTGAGACAATGGGTTCTGCTACTTGTATTTGCACAGATAAAACTGGAACCTTGACAACAAATCATATGGTTGTGAACAAGATATGGGTCTGTAACGAAACCAAGACTATAACGAGTAATGAGAACAATGACGTGTTGAAGTCAGCTTCTGAAGGAGTACATAACCTCCTTTTACAGTCCATCTTTCTGAATACAAGCGCGGAGGTAGTCAAGGGGGAAGATGGAAAGAATAGCATTATGGGGACTCCAACAGAGACGGCGATAATTGAATTTGGAATGCTTCTGGGAGGTGATTTTAAATCTTACAGTGAGGATTATAAGATACTTACGGTTGAACCATTCAATTCAGTCAGGAAAAAGATGTTGGTGCTAGTGGCTCTACCTGATGGAGGAAAGAAACGAGCATTCTGCAAAGGAGCATCAGAAATAATCTTAGAAATGTGTGATAATGTTGTTGGCACTGATGGTGAAGCTGTGCCTCTATCAGACGAGCAAAGAACGAATATCTCAGATATCATAAATGGTTTTGCTTGTGAAGCTTTAAGAACTCTATGCTTAGCTTTCAAGAATATAGATGATTCCTCTAGTGAAAACAGTATTCCTGACGATAAATATACGTTAATAGCTGTTGTTGGAATCAAGGATCCTGTGCGTCCAGGAGTGAGGGAAGCAGTGAAGACTTGTTTAGATGCTGGGATTACTGTAAGGATGGTCACCGGTGACAATATTAATACGGCTAAAGCCATAGCTACGGAGTGTGGTATTTTGACAGAAGGTGGTTTGGCTATAGAAGGGCCAGATTTCCGAAATAAGAGCTCACAGGAGATGGCGGAAATAATTCCGAAACTGCAGGTAATGGCTCGATCCTTGCCTTTGGACAAGCACACCTTGGTGAAACAGTTGAGGAATGAGCTTAGAGAAGTTGTGGCAGTAACTGGTGATGGGACGAATGACGCTCCTGCGCTGCACGAGGCAGACATCGGACTTGCCATGGGTATTGCGGGAACGGAGGTTGCGAAAGAGAATGCCGATGTTATCATAATGGATGACAACTTTACAAGCATAGTGAACGTTGCAAGATGGGGTCGGGCGGTTTACATCAACATTCAAAAGTTTGTACAGTTCCAACTAACAGTTAACATCGCAGCTCTGATGCTCAATTTCGTTTCAGCATGCGTCTCAGGATCCGCGCCTCTCACGGCTGTCCAGATGCTTTGGGTGAACTTGATCATGGATACTCTTGGTGCTTTGGCGCTGGCTACGGAGCCACCAAACGAGGGTCTAATGAAAAGACCACCGGTCGGAAGGAACGTCAAGTTCATAACCGGGATCATGTGGAGGAACATTGTTGGCCAGAGTATCTACCAAATTGCTGTCCTTCTGGTTCTCAAGTTTTGCGGACTCCGGCTTTTGAAGCTAACTGGTTCCGATGCAAATTCCGTTCTCAATACCGTCATATTCAACTCTTTTGTGTTTTGCCAGGTATTCAACGAGATAAACAGCCGTGATATGGAGAAGATCAATGTGTTCAGTGGCATGTTCAGCAGTTACACCTTCATGATGGTGATGATCTCCACCGTTGTTTTCCAGATCATCATCGTTGAATTCTTGGGAAAGTTTGCACAAACCGTGCCACTGAGCTGGGAGTTGTGGTTAACAAGCGTCTTGATCGGCGCTGTAAGTTTGCTCGTCGCTGTCGTCGTCAAGTTCATTCCTGTTTCTGTCATGAAACAACAAGCTCCTCGGGATCACGAGGATGTCTACGAACCTCTCCTCCGTGGCCCTGACAATGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0001101 GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006950 GO:0006952 GO:0008150 GO:0008324 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009705 GO:0010035 GO:0010941 GO:0012505 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0031090 GO:0031224 GO:0031226 GO:0034220 GO:0042221 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0042742 GO:0043067 GO:0043069 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048519 GO:0048523 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055081 GO:0055085 GO:0060548 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098542 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098805 GO:0099131 GO:0099132 GO:1901700
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CaATP_NAI Cation_ATPase Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase Hydrolase_3

Protein Analysis

1042

Amino Acids

113.78

Weight (kDa)

6.08

Isoelectric Point (pI)

37.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CaATP_NAI PF12515 11 - 53 2e-20 Ca2+-ATPase N terminal autoinhibitory domain
Cation_ATPase_N PF00690 120 - 186 1.1e-10 Cation transporter/ATPase, N-terminus
E1-E2_ATPase PF00122 238 - 333 4.4e-22 P-type ATPase actuator domain
Cation_ATPase PF13246 500 - 662 5.8e-35 P-type ATPase, cytoplasmic domain N
Hydrolase PF00702 647 - 769 7.9e-12 haloacid dehalogenase-like hydrolase
Cation_ATPase_C PF00689 840 - 1014 5e-42 Cation transporting ATPase, C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015798)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g01980 FvH4_7g01980
malus_domestica MD02G1298100.v1.1 MD07G1026000.v1.1
prunus_persica Prupe.2G026200_v2.0.a1
pyrus_communis pycom07g01980
rosa_chinensis RchiOBHm_Chr1g0318981
rosa_laevigata RLG00000030512
rosa_multiflora Rmu_sc0003503.1_g000001 Rmu_sc0006626.1_g000004
rosa_roxburghii Rroxscaffold_4G00328710
rosa_rugosa Rorug01G0026100 Rorug01G0026200
rosa_samantha Rh1AG038000
rosa_wichuraiana Rw1G002930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1220, 1647
AarI CACCTGC 1 cut(s) 1030
AatII GACGTC 1 cut(s) 45
Acc36I ACCTGC 2 cut(s) 1030, 2170
AccB7I CCANNNNNTGG 1 cut(s) 818
AccBSI CCGCTC 1 cut(s) 166
AccI GTMKAC 3 cut(s) 742, 852, 3090
AccII CGCG 5 cut(s) 92, 153, 1094, 1529, 2519
AclI AACGTT 1 cut(s) 2400
AclWI GGATC 9 cut(s) 81, 296, 1967, 1980, 2187, 2508, 2521, 2666, 3084
AcoI YGGCCR 2 cut(s) 56, 2680
AcsI RAATTY 3 cut(s) 1598, 2764, 2933
AcuI CTGAAG 4 cut(s) 95, 327, 600, 1500
AcyI GRCGYC 1 cut(s) 42
AfaI GTAC 3 cut(s) 213, 1488, 2448
AfiI CCNNNNNNNGG 7 cut(s) 78, 482, 818, 1718, 2606, 2630, 2839
AflIII ACRYGT 2 cut(s) 196, 1461
AgeI ACCGGT 2 cut(s) 2045, 2626
AhdI GACNNNNNGTC 3 cut(s) 749, 1266, 2627
AjiI CACGTC 1 cut(s) 1462
AjnI CCWGG 3 cut(s) 183, 1984, 2808
AjuI GAANNNNNNNTTGG 2 cut(s) 1676, 1708
AleI CACNNNNGTG 1 cut(s) 2219
AloI GAACNNNNNNTCC 4 cut(s) 768, 800, 3087, 3119
Alw21I GWGCWC 1 cut(s) 2147
Alw26I GTCTC 8 cut(s) 559, 726, 757, 1014, 1022, 1328, 1577, 2512
AlwI GGATC 9 cut(s) 81, 296, 1967, 1980, 2187, 2508, 2521, 2666, 3084
AlwNI CAGNNNCTG 3 cut(s) 676, 1478, 2264
Ama87I CYCGRG 1 cut(s) 3072
AoxI GGCC 6 cut(s) 56, 69, 835, 2123, 2680, 3109
ApeKI GCWGC 4 cut(s) 545, 548, 2293, 2474
ApoI RAATTY 3 cut(s) 1598, 2764, 2933
AseI ATTAAT 1 cut(s) 2058
AsiGI ACCGGT 2 cut(s) 2045, 2626
Asp700I GAANNNNTTC 2 cut(s) 783, 2166
AspLEI GCGC 8 cut(s) 153, 155, 174, 1529, 2293, 2521, 2584, 3005
AspS9I GGNCC 4 cut(s) 70, 978, 2123, 3110
AsuC2I CCSGG 1 cut(s) 2656
AvaI CYCGRG 1 cut(s) 3072
AvaII GGWCC 1 cut(s) 978
BalI TGGCCA 1 cut(s) 2682
BamHI GGATCC 2 cut(s) 1972, 2513
BanII GRGCYC 1 cut(s) 2147
BauI CACGAG 2 cut(s) 2297, 3080
BbsI GAAGAC 2 cut(s) 1161, 2012
Bbv12I GWGCWC 1 cut(s) 2147
BbvI GCAGC 4 cut(s) 532, 535, 2280, 2486
BceAI ACGGC 7 cut(s) 43, 71, 1196, 1602, 2079, 2544, 2817
BcgI CGANNNNNNTGC 2 cut(s) 1719, 1753
BciT130I CCWGG 3 cut(s) 185, 1986, 2810
BciVI GTATCC 1 cut(s) 2557
BclI TGATCA 2 cut(s) 802, 2556
BcnI CCSGG 1 cut(s) 2656
BcoDI GTCTC 8 cut(s) 559, 726, 757, 1014, 1022, 1328, 1577, 2512
BfaI CTAG 3 cut(s) 729, 1700, 1914
BfmI CTRYAG 4 cut(s) 558, 1037, 2115, 2177
BfoI RGCGCY 2 cut(s) 2585, 3006
BfuAI ACCTGC 2 cut(s) 1030, 2170
BfuI GTATCC 1 cut(s) 2557
BisI GCNGC 5 cut(s) 222, 546, 549, 2294, 2475
BlpI GCTNAGC 1 cut(s) 1885
BlsI GCNGC 5 cut(s) 223, 547, 550, 2295, 2476
Bme1390I CCNGG 4 cut(s) 185, 1986, 2656, 2810
Bme18I GGWCC 1 cut(s) 978
BmeRI GACNNNNNGTC 3 cut(s) 749, 1266, 2627
BmeT110I CYCGRG 1 cut(s) 3072
BmgBI CACGTC 1 cut(s) 1462
BmgT120I GGNCC 4 cut(s) 70, 978, 2123, 3110
BmiI GGNNCC 7 cut(s) 125, 925, 1378, 1974, 2515, 2595, 2755
BmrFI CCNGG 4 cut(s) 185, 1986, 2656, 2810
BoxI GACNNNNGTC 1 cut(s) 1211
BpiI GAAGAC 2 cut(s) 1161, 2012
BpmI CTGGAG 1 cut(s) 167
Bpu1102I GCTNAGC 1 cut(s) 1885
BpuEI CTTGAG 1 cut(s) 2702
BpuMI CCSGG 1 cut(s) 2656
Bsa29I ATCGAT 1 cut(s) 237
BsaAI YACGTR 2 cut(s) 197, 211
BsaBI GATNNNNATC 1 cut(s) 2924
BsaHI GRCGYC 1 cut(s) 42
BsaI GGTCTC 2 cut(s) 559, 757
BsaJI CCNNGG 7 cut(s) 63, 90, 156, 2217, 2319, 3071, 3106
BsaWI WCCGGW 2 cut(s) 2045, 2626
BsaXI ACNNNNNCTCC 8 cut(s) 768, 798, 1479, 1509, 2966, 2996, 3087, 3117
Bsc4I CCNNNNNNNGG 7 cut(s) 78, 482, 818, 1718, 2606, 2630, 2839
Bse118I RCCGGY 2 cut(s) 2045, 2626
Bse1I ACTGG 6 cut(s) 696, 884, 1169, 1378, 2269, 2755
Bse3DI GCAATG 2 cut(s) 224, 1287
Bse8I GATNNNNATC 1 cut(s) 2924
BseBI CCWGG 3 cut(s) 185, 1986, 2810
BseCI ATCGAT 1 cut(s) 237
BseDI CCNNGG 7 cut(s) 63, 90, 156, 2217, 2319, 3071, 3106
BseGI GGATG 4 cut(s) 317, 2043, 2380, 3091
BseJI GATNNNNATC 1 cut(s) 2924
BseLI CCNNNNNNNGG 7 cut(s) 78, 482, 818, 1718, 2606, 2630, 2839
BseMI GCAATG 2 cut(s) 224, 1287
BseMII CTCAG 4 cut(s) 998, 1851, 2523, 2957
BseNI ACTGG 6 cut(s) 696, 884, 1169, 1378, 2269, 2755
BsePI GCGCGC 1 cut(s) 151
BseRI GAGGAG 4 cut(s) 80, 378, 3060, 3092
BseX3I CGGCCG 1 cut(s) 56
BseXI GCAGC 4 cut(s) 532, 535, 2280, 2486
BseYI CCCAGC 2 cut(s) 2021, 2970
BsgI GTGCAG 1 cut(s) 2279
Bsh1236I CGCG 5 cut(s) 92, 153, 1094, 1529, 2519
Bsh1285I CGRYCG 2 cut(s) 59, 2631
BshFI GGCC 6 cut(s) 58, 71, 837, 2125, 2682, 3111
BshTI ACCGGT 2 cut(s) 2045, 2626
BshVI ATCGAT 1 cut(s) 237
BsiEI CGRYCG 2 cut(s) 59, 2631
BsiHKAI GWGCWC 1 cut(s) 2147
BsiHKCI CYCGRG 1 cut(s) 3072
BsiSI CCGG 4 cut(s) 2046, 2627, 2655, 2734
BslFI GGGAC 4 cut(s) 1002, 1220, 1585, 2287
BslI CCNNNNNNNGG 7 cut(s) 78, 482, 818, 1718, 2606, 2630, 2839
BsmAI GTCTC 8 cut(s) 559, 726, 757, 1014, 1022, 1328, 1577, 2512
BsmBI CGTCTC 3 cut(s) 726, 1577, 2512
BsmFI GGGAC 4 cut(s) 1002, 1220, 1585, 2287
BsmI GAATGC 4 cut(s) 591, 1611, 1733, 2359
BsnI GGCC 6 cut(s) 58, 71, 837, 2125, 2682, 3111
Bso31I GGTCTC 2 cut(s) 559, 757
BsoBI CYCGRG 1 cut(s) 3072
Bsp1286I GDGCHC 1 cut(s) 2147
Bsp1407I TGTACA 1 cut(s) 2446
Bsp1720I GCTNAGC 1 cut(s) 1885
Bsp19I CCATGG 1 cut(s) 2319
BspANI GGCC 6 cut(s) 58, 71, 837, 2125, 2682, 3111
BspCNI CTCAG 4 cut(s) 999, 1850, 2522, 2958
BspDI ATCGAT 1 cut(s) 237
BspFNI CGCG 5 cut(s) 92, 153, 1094, 1529, 2519
BspHI TCATGA 3 cut(s) 334, 2886, 3054
BspLI GGNNCC 7 cut(s) 125, 925, 1378, 1974, 2515, 2595, 2755
BspMAI CTGCAG 2 cut(s) 1041, 2181
BspMI ACCTGC 2 cut(s) 1030, 2170
BspPI GGATC 9 cut(s) 81, 296, 1967, 1980, 2187, 2508, 2521, 2666, 3084
BspTNI GGTCTC 2 cut(s) 559, 757
BsrBI CCGCTC 1 cut(s) 166
BsrDI GCAATG 2 cut(s) 224, 1287
BsrFI RCCGGY 2 cut(s) 2045, 2626
BsrGI TGTACA 1 cut(s) 2446
BsrI ACTGG 6 cut(s) 696, 884, 1169, 1378, 2269, 2755
BssAI RCCGGY 2 cut(s) 2045, 2626
BssECI CCNNGG 7 cut(s) 63, 90, 156, 2217, 2319, 3071, 3106
BssHII GCGCGC 1 cut(s) 151
BssNI GRCGYC 1 cut(s) 42
BssSI CACGAG 2 cut(s) 2297, 3080
BssT1I CCWWGG 2 cut(s) 2217, 2319
Bst2BI CACGAG 2 cut(s) 2297, 3080
Bst2UI CCWGG 3 cut(s) 185, 1986, 2810
BstACI GRCGYC 1 cut(s) 42
BstAPI GCANNNNNTGC 2 cut(s) 323, 676
BstAUI TGTACA 1 cut(s) 2446
BstBAI YACGTR 2 cut(s) 197, 211
BstC8I GCNNGC 4 cut(s) 153, 244, 2503, 2586
BstDEI CTNAG 7 cut(s) 1007, 1762, 1837, 1885, 2243, 2509, 2966
BstDSI CCRYGG 3 cut(s) 90, 2319, 3106
BstEII GGTNACC 1 cut(s) 2041
BstENI CCTNNNNNAGG 2 cut(s) 480, 1716
BstF5I GGATG 4 cut(s) 317, 2043, 2380, 3091
BstFNI CGCG 5 cut(s) 92, 153, 1094, 1529, 2519
BstH2I RGCGCY 2 cut(s) 2585, 3006
BstHHI GCGC 8 cut(s) 153, 155, 174, 1529, 2293, 2521, 2584, 3005
BstMAI GTCTC 8 cut(s) 559, 726, 757, 1014, 1022, 1328, 1577, 2512
BstMCI CGRYCG 2 cut(s) 59, 2631
BstMWI GCNNNNNNNGC 8 cut(s) 143, 152, 323, 676, 870, 1865, 2743, 2871
BstNI CCWGG 3 cut(s) 185, 1986, 2810
BstNSI RCATGY 3 cut(s) 1332, 2505, 2869
BstPAI GACNNNNGTC 1 cut(s) 1211
BstPI GGTNACC 1 cut(s) 2041
BstSCI CCNGG 4 cut(s) 183, 1984, 2654, 2808
BstSFI CTRYAG 4 cut(s) 558, 1037, 2115, 2177
BstSNI TACGTA 1 cut(s) 211
BstUI CGCG 5 cut(s) 92, 153, 1094, 1529, 2519
BstV1I GCAGC 4 cut(s) 532, 535, 2280, 2486
BstV2I GAAGAC 2 cut(s) 1161, 2012
BstX2I RGATCY 3 cut(s) 86, 1972, 2513
BstXI CCANNNNNNTGG 1 cut(s) 2970
BstYI RGATCY 3 cut(s) 86, 1972, 2513
BstZI CGGCCG 1 cut(s) 56
Bsu15I ATCGAT 1 cut(s) 237
BsuI GTATCC 1 cut(s) 2557
BsuRI GGCC 6 cut(s) 58, 71, 837, 2125, 2682, 3111
BsuTUI ATCGAT 1 cut(s) 237
BtgI CCRYGG 3 cut(s) 90, 2319, 3106
BtgZI GCGATG 1 cut(s) 2455
BtrI CACGTC 1 cut(s) 1462
BtsCI GGATG 4 cut(s) 317, 2043, 2380, 3091
BtsI GCAGTG 4 cut(s) 674, 1034, 1263, 2007
BtsIMutI CAGTG 9 cut(s) 674, 891, 1034, 1263, 1642, 1788, 2007, 2866, 2963
BveI ACCTGC 2 cut(s) 1030, 2170
Cac8I GCNNGC 4 cut(s) 153, 244, 2503, 2586
CaiI CAGNNNCTG 3 cut(s) 676, 1478, 2264
CciI TCATGA 3 cut(s) 334, 2886, 3054
CfoI GCGC 8 cut(s) 153, 155, 174, 1529, 2293, 2521, 2584, 3005
Cfr10I RCCGGY 2 cut(s) 2045, 2626
Cfr13I GGNCC 4 cut(s) 70, 978, 2123, 3110
Cfr42I CCGCGG 1 cut(s) 93
ClaI ATCGAT 1 cut(s) 237
CseI GACGC 5 cut(s) 40, 1970, 2291, 2494, 2980
Csp6I GTAC 3 cut(s) 212, 1487, 2447
CspAI ACCGGT 2 cut(s) 2045, 2626
CviQI GTAC 3 cut(s) 212, 1487, 2447
DdeI CTNAG 7 cut(s) 1007, 1762, 1837, 1885, 2243, 2509, 2966
DraI TTTAAA 2 cut(s) 685, 1627
DriI GACNNNNNGTC 3 cut(s) 749, 1266, 2627
EaeI YGGCCR 2 cut(s) 56, 2680
EagI CGGCCG 1 cut(s) 56
Eam1105I GACNNNNNGTC 3 cut(s) 749, 1266, 2627
EciI GGCGGA 1 cut(s) 2174
Ecl136II GAGCTC 1 cut(s) 2145
EclXI CGGCCG 1 cut(s) 56
Eco105I TACGTA 1 cut(s) 211
Eco130I CCWWGG 2 cut(s) 2217, 2319
Eco24I GRGCYC 1 cut(s) 2147
Eco31I GGTCTC 2 cut(s) 559, 757
Eco32I GATATC 1 cut(s) 1843
Eco47I GGWCC 1 cut(s) 978
Eco52I CGGCCG 1 cut(s) 56
Eco53kI GAGCTC 1 cut(s) 2145
Eco57I CTGAAG 4 cut(s) 95, 327, 600, 1500
Eco88I CYCGRG 1 cut(s) 3072
Eco91I GGTNACC 1 cut(s) 2041
EcoICRI GAGCTC 1 cut(s) 2145
EcoNI CCTNNNNNAGG 2 cut(s) 480, 1716
EcoO109I RGGNCCY 1 cut(s) 70
EcoO65I GGTNACC 1 cut(s) 2041
EcoRI GAATTC 1 cut(s) 2933
EcoRII CCWGG 3 cut(s) 183, 1984, 2808
EcoRV GATATC 1 cut(s) 1843
EcoT14I CCWWGG 2 cut(s) 2217, 2319
EcoT22I ATGCAT 1 cut(s) 3124
EcoT38I GRGCYC 1 cut(s) 2147
ErhI CCWWGG 2 cut(s) 2217, 2319
Esp3I CGTCTC 3 cut(s) 726, 1577, 2512
FalI AAGNNNNNCTT 2 cut(s) 2201, 2233
FaqI GGGAC 4 cut(s) 1002, 1220, 1585, 2287
FauI CCCGC 1 cut(s) 2323
FauNDI CATATG 1 cut(s) 1395
FbaI TGATCA 2 cut(s) 802, 2556
FblI GTMKAC 3 cut(s) 742, 852, 3090
Fnu4HI GCNGC 5 cut(s) 222, 546, 549, 2294, 2475
FokI GGATG 4 cut(s) 304, 2050, 2387, 3098
FriOI GRGCYC 1 cut(s) 2147
Fsp4HI GCNGC 5 cut(s) 222, 546, 549, 2294, 2475
FspBI CTAG 3 cut(s) 729, 1700, 1914
GlaI GCGC 8 cut(s) 152, 154, 173, 1528, 2292, 2520, 2583, 3004
GluI GCNGC 5 cut(s) 222, 546, 549, 2294, 2475
GsaI CCCAGC 2 cut(s) 2025, 2974
GsuI CTGGAG 1 cut(s) 167
HaeII RGCGCY 2 cut(s) 2585, 3006
HaeIII GGCC 6 cut(s) 58, 71, 837, 2125, 2682, 3111
HapII CCGG 4 cut(s) 2046, 2627, 2655, 2734
HgaI GACGC 5 cut(s) 40, 1970, 2291, 2494, 2980
HhaI GCGC 8 cut(s) 153, 155, 174, 1529, 2293, 2521, 2584, 3005
Hin1I GRCGYC 1 cut(s) 42
Hin6I GCGC 8 cut(s) 151, 153, 172, 1527, 2291, 2519, 2582, 3003
HinP1I GCGC 8 cut(s) 151, 153, 172, 1527, 2291, 2519, 2582, 3003
HincII GTYRAC 3 cut(s) 853, 2467, 2985
HindII GTYRAC 3 cut(s) 853, 2467, 2985
HindIII AAGCTT 2 cut(s) 862, 1866
HinfI GANTC 6 cut(s) 109, 857, 1573, 1907, 1965, 2730
HpaI GTTAAC 2 cut(s) 2467, 2985
HpaII CCGG 4 cut(s) 2046, 2627, 2655, 2734
Hpy99I CGWCG 5 cut(s) 44, 47, 857, 3023, 3032
HpyCH4IV ACGT 8 cut(s) 42, 196, 210, 739, 1461, 1946, 2400, 2640
HpyF10VI GCNNNNNNNGC 8 cut(s) 143, 152, 323, 676, 870, 1865, 2743, 2871
HpyF3I CTNAG 7 cut(s) 1007, 1762, 1837, 1885, 2243, 2509, 2966
HpySE526I ACGT 8 cut(s) 42, 196, 210, 739, 1461, 1946, 2400, 2640
Hsp92I GRCGYC 1 cut(s) 42
HspAI GCGC 8 cut(s) 151, 153, 172, 1527, 2291, 2519, 2582, 3003
Ksp22I TGATCA 2 cut(s) 802, 2556
KspAI GTTAAC 2 cut(s) 2467, 2985
KspI CCGCGG 1 cut(s) 93
LmnI GCTCC 4 cut(s) 1745, 2290, 2593, 3073
Lsp1109I GCAGC 4 cut(s) 532, 535, 2280, 2486
MaeI CTAG 3 cut(s) 729, 1700, 1914
MaeII ACGT 8 cut(s) 42, 196, 210, 739, 1461, 1946, 2400, 2640
MbiI CCGCTC 1 cut(s) 166
MboII GAAGA 7 cut(s) 320, 715, 905, 1161, 1559, 2017, 2857
MflI RGATCY 3 cut(s) 86, 1972, 2513
MhlI GDGCHC 1 cut(s) 2147
MlsI TGGCCA 1 cut(s) 2682
MluNI TGGCCA 1 cut(s) 2682
MlyI GAGTC 2 cut(s) 1567, 2724
MmeI TCCRAC 7 cut(s) 547, 754, 1278, 1601, 1942, 2479, 2611
Mox20I TGGCCA 1 cut(s) 2682
Mph1103I ATGCAT 1 cut(s) 3124
MroXI GAANNNNTTC 2 cut(s) 783, 2166
MscI TGGCCA 1 cut(s) 2682
MslI CAYNNNNRTG 4 cut(s) 2219, 2885, 2891, 3084
Msp20I TGGCCA 1 cut(s) 2682
MspA1I CMGCKG 2 cut(s) 92, 815
MspI CCGG 4 cut(s) 2046, 2627, 2655, 2734
MspR9I CCNGG 4 cut(s) 185, 1986, 2656, 2810
Mva1269I GAATGC 4 cut(s) 591, 1611, 1733, 2359
MvaI CCWGG 3 cut(s) 185, 1986, 2810
MvnI CGCG 5 cut(s) 92, 153, 1094, 1529, 2519
MwoI GCNNNNNNNGC 8 cut(s) 143, 152, 323, 676, 870, 1865, 2743, 2871
NciI CCSGG 1 cut(s) 2656
NcoI CCATGG 1 cut(s) 2319
NdeI CATATG 1 cut(s) 1395
NlaIV GGNNCC 7 cut(s) 125, 925, 1378, 1974, 2515, 2595, 2755
NmeAIII GCCGAG 1 cut(s) 181
NmuCI GTSAC 7 cut(s) 646, 672, 724, 958, 1258, 2041, 2048
NsiI ATGCAT 1 cut(s) 3124
NspI RCATGY 3 cut(s) 1332, 2505, 2869
OliI CACNNNNGTG 1 cut(s) 2219
PaeI GCATGC 1 cut(s) 2505
PagI TCATGA 3 cut(s) 334, 2886, 3054
PaqCI CACCTGC 1 cut(s) 1030
PauI GCGCGC 1 cut(s) 151
PcsI WCGNNNNNNNCGW 4 cut(s) 2342, 2637, 3024, 3027
PctI GAATGC 4 cut(s) 591, 1611, 1733, 2359
PdmI GAANNNNTTC 2 cut(s) 783, 2166
PfeI GAWTC 4 cut(s) 109, 857, 1907, 1965
PflMI CCANNNNNTGG 1 cut(s) 818
PfoI TCCNGGA 2 cut(s) 183, 1984
PinAI ACCGGT 2 cut(s) 2045, 2626
PkrI GCNGC 5 cut(s) 223, 547, 550, 2295, 2476
PleI GAGTC 2 cut(s) 1567, 2724
PpsI GAGTC 2 cut(s) 1567, 2724
Ppu21I YACGTR 2 cut(s) 197, 211
PshAI GACNNNNGTC 1 cut(s) 1211
PshBI ATTAAT 1 cut(s) 2058
PsiI TTATAA 2 cut(s) 1220, 1647
Psp124BI GAGCTC 1 cut(s) 2147
Psp1406I AACGTT 1 cut(s) 2400
Psp6I CCWGG 3 cut(s) 183, 1984, 2808
PspEI GGTNACC 1 cut(s) 2041
PspFI CCCAGC 2 cut(s) 2021, 2970
PspGI CCWGG 3 cut(s) 183, 1984, 2808
PspN4I GGNNCC 7 cut(s) 125, 925, 1378, 1974, 2515, 2595, 2755
PspPI GGNCC 4 cut(s) 70, 978, 2123, 3110
PstI CTGCAG 2 cut(s) 1041, 2181
PstNI CAGNNNCTG 3 cut(s) 676, 1478, 2264
PsuI RGATCY 3 cut(s) 86, 1972, 2513
PteI GCGCGC 1 cut(s) 151
PvuII CAGCTG 1 cut(s) 815
RsaI GTAC 3 cut(s) 213, 1488, 2448
RsaNI GTAC 3 cut(s) 212, 1487, 2447
RseI CAYNNNNRTG 4 cut(s) 2219, 2885, 2891, 3084
SacI GAGCTC 1 cut(s) 2147
SacII CCGCGG 1 cut(s) 93
SalI GTCGAC 1 cut(s) 851
SatI GCNGC 5 cut(s) 222, 546, 549, 2294, 2475
Sau96I GGNCC 4 cut(s) 70, 978, 2123, 3110
SchI GAGTC 2 cut(s) 1567, 2724
ScrFI CCNGG 4 cut(s) 185, 1986, 2656, 2810
SduI GDGCHC 1 cut(s) 2147
SfcI CTRYAG 4 cut(s) 558, 1037, 2115, 2177
Sfr303I CCGCGG 1 cut(s) 93
SgrAI CRCCGGYG 1 cut(s) 2045
SgrBI CCGCGG 1 cut(s) 93
SinI GGWCC 1 cut(s) 978
SmiMI CAYNNNNRTG 4 cut(s) 2219, 2885, 2891, 3084
SmlI CTYRAG 1 cut(s) 2717
SmoI CTYRAG 1 cut(s) 2717
SnaBI TACGTA 1 cut(s) 211
SphI GCATGC 1 cut(s) 2505
SspI AATATT 1 cut(s) 2056
SspMI CTAG 3 cut(s) 729, 1700, 1914
SstI GAGCTC 1 cut(s) 2147
StyD4I CCNGG 4 cut(s) 183, 1984, 2654, 2808
StyI CCWWGG 2 cut(s) 2217, 2319
TaiI ACGT 8 cut(s) 45, 199, 213, 742, 1464, 1949, 2403, 2643
TaqI TCGA 7 cut(s) 39, 45, 237, 328, 852, 1205, 2191
TaqII GACCGA 1 cut(s) 995
TatI WGTACW 2 cut(s) 1486, 2446
TauI GCSGC 1 cut(s) 224
TfiI GAWTC 4 cut(s) 109, 857, 1907, 1965
TscAI CASTG 9 cut(s) 681, 891, 1041, 1263, 1642, 1795, 2007, 2866, 2970
TseFI GTSAC 7 cut(s) 646, 672, 724, 958, 1258, 2041, 2048
TseI GCWGC 4 cut(s) 545, 548, 2293, 2474
Tsp45I GTSAC 7 cut(s) 646, 672, 724, 958, 1258, 2041, 2048
TspGWI ACGGA 7 cut(s) 375, 750, 2096, 2351, 2606, 2758, 3095
TspRI CASTG 9 cut(s) 681, 891, 1041, 1263, 1642, 1795, 2007, 2866, 2970
Van91I CCANNNNNTGG 1 cut(s) 818
VpaK11BI GGWCC 1 cut(s) 978
VspI ATTAAT 1 cut(s) 2058
XagI CCTNNNNNAGG 2 cut(s) 480, 1716
XapI RAATTY 3 cut(s) 1598, 2764, 2933
XceI RCATGY 3 cut(s) 1332, 2505, 2869
XmiI GTMKAC 3 cut(s) 742, 852, 3090
XmnI GAANNNNTTC 2 cut(s) 783, 2166
XspI CTAG 3 cut(s) 729, 1700, 1914
ZraI GACGTC 1 cut(s) 43
Zsp2I ATGCAT 1 cut(s) 3124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.