FvH4_7g02030

Myosin heavy chain kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0014503
Physical Location & Seq
Forward (+)
2397647 .. 2399458
1812 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g02030.t1

Sequence Viewer

Length: 1251 bp
ATGTTTCATCATCCCATGATGACTCTCTCAGAAAACAAACGTCTCTCAGTCACACCCTTGCTCTCTGCAGCCTCATCAGCAACAACAACAAGCAGCGACGCCTCCGACGATAGCCCGCCGGCCTCGGCACGTTTCGAGATCAGCTACCATCAGTTTCCATGCACGGCGCACTTATTATCCGGGTCCTTCTTCTCCTATCGATCACTGGCTGTCCTTTCCGGCCACAAAGGCTCCATTTCGTGCCTGTCTTTATGCGGAGAGTTCATCCTCAGTGCTTCACAAGGCAAAGACATCATCGTGTGGCAGCAGCCTGATCTGAGGCCTTTCACAAAGTTCGGACAAGGCGATGGCTCGGTGAAGGCGGTTGTGACCGTTGGGAACAAGGTCTTCACGGCGCACCAAGACAGCAGGATCAGAGTTTGGAAGGTTTCGCGGAGCTCGGAGAATGTGTTTAGGCTTGTCGATACTCTGCCTACAACGAAAGATTATTTGGGGAAGTTCATGAAGCAAAGCAACTATGTTCAAACGAGGAGGCATCACAAGAGGTTGTGGATTGAACATGCTGATAGTATTTCTTGTCTGGCTTTCTATAATGGGTTGGTTTACTCTGGATCTTGGGATAAGACCCTTAAGGTGTGGAGGGTTTCGGATTTGAAGTGCGTGGAGTCGATCAAGGCTCATGACGATGCCATTAATGGGTTGGCTGCAAGTAAAGGGATTGTGTATTCTGCTTCTGCTGATGGAAAAATCAAGGCTTGGGGAAAAGAAGGCAAGAGCTCACATTGCTTGAAGGGTATCTTGGAGGGTCATAAGGATGTTTCGTTTAACTCGGTGGTGGTTTCAGAGGATGGGAGATGGGTCTATGGAGGCGGTTCCGATGGATTTGTGATGGGGTGGGAAGGGTGTACAAATGGTGGTGATCAAATTGGGAGTTGGAAGTTGGTTTGTGAAACAAAAGCGCATCAAATGGCAGTGTTGTCGATGTGTTTGATGGGGGAGATGTTGTGTAGTGGATCAGCAGATAAGAGTATAGGTATCTGGAAGAGAGAAGCTTTTGGGAAGCTTTCCAAAATTGGGGTTATTAGCGGCCATGAAGGGCCAGTCAAGTGTTTGCAAGCATCGCCGAATTGTGTCGGTGGTGGTTTCATGCTGTATAGTGGAGGCCTTGACAAGAGCTTAAGAGTTTGGTGGGTTTCCAAACCCTCCACAACACAGACGAGTACAGAGGAGAAGTCTGTCATGTTGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

417

Amino Acids

45.28

Weight (kDa)

8.95

Isoelectric Point (pI)

36.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 72 - 274 2.8e-18 WDR5 beta-propeller domain
Beta-prop_SCAP PF24017 73 - 254 7.2e-07 SCAP Beta-propeller
WD40_WDHD1_1st PF24817 124 - 267 7.3e-06 WDHD1 first WD40 domain
WDR55 PF24796 175 - 399 1.2e-07 WDR55
Beta-prop_WDR3_1st PF25173 185 - 300 4.6e-11 WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd PF25047 186 - 258 4.3e-06 TEP-1 second beta-propeller
WD40_CDC20-Fz PF24807 186 - 324 3.8e-07 CDC20/Fizzy WD40 domain
Beta-prop_EML PF23409 187 - 372 4.9e-09 Echinoderm microtubule-associated protein first beta-propeller
WD40_Prp19 PF24814 190 - 408 4.3e-16 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 198 - 300 1.2e-08 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 200 - 299 1.7e-07 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 268 - 405 1.4e-09 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 288 - 403 1.8e-10 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 313 - 412 7.3e-07 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 314 - 406 5.5e-06 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 326 - 412 5.6e-08 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014503)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51930
fragaria_vesca FvH4_7g02030
malus_domestica MD02G1296500.v1.1 MD07G1027800.v1.1
prunus_persica Prupe.2G027900_v2.0.a1 Prupe.2G027900_v2.0.a1
pyrus_communis pycom02g24950 pycom07g02110
rosa_chinensis RchiOBHm_Chr1g0319521
rosa_laevigata RLG00000030475
rosa_multiflora Rmu_sc0002539.1_g000012
rosa_roxburghii Rroxscaffold_4G00328310
rosa_samantha Rh1AG040100 Rh1BG037000 Rh1CG041100 Rh1DG028800
rosa_wichuraiana Rw1G003240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 433
AciI CCGC 6 cut(s) 116, 255, 362, 433, 870, 1086
AclWI GGATC 3 cut(s) 419, 619, 1021
AcoI YGGCCR 2 cut(s) 220, 1087
AcyI GRCGYC 1 cut(s) 99
AfaI GTAC 2 cut(s) 907, 1222
AfiI CCNNNNNNNGG 2 cut(s) 696, 1074
AflII CTTAAG 2 cut(s) 629, 1177
AgsI TTSAA 4 cut(s) 524, 557, 655, 790
AjuI GAANNNNNNNTTGG 4 cut(s) 473, 505, 782, 814
AluBI AGCT 7 cut(s) 144, 438, 777, 1052, 1063, 1176, 1248
AluI AGCT 7 cut(s) 144, 438, 777, 1052, 1063, 1176, 1248
Alw21I GWGCWC 2 cut(s) 440, 779
Alw26I GTCTC 1 cut(s) 47
AlwI GGATC 3 cut(s) 419, 619, 1021
AoxI GGCC 6 cut(s) 120, 220, 320, 1087, 1097, 1162
ApeKI GCWGC 5 cut(s) 68, 93, 304, 307, 704
AseI ATTAAT 1 cut(s) 693
AspLEI GCGC 3 cut(s) 169, 397, 961
AspS9I GGNCC 2 cut(s) 183, 1097
AsuC2I CCSGG 1 cut(s) 181
AsuHPI GGTGA 2 cut(s) 367, 929
AvaII GGWCC 1 cut(s) 183
BanII GRGCYC 2 cut(s) 440, 779
BbsI GAAGAC 1 cut(s) 379
Bbv12I GWGCWC 2 cut(s) 440, 779
BbvI GCAGC 5 cut(s) 80, 105, 316, 319, 691
BccI CCATC 8 cut(s) 156, 341, 734, 842, 849, 872, 883, 985
BceAI ACGGC 2 cut(s) 180, 408
BcgI CGANNNNNNTGC 2 cut(s) 960, 994
BclI TGATCA 1 cut(s) 919
BcnI CCSGG 1 cut(s) 181
BcoDI GTCTC 1 cut(s) 47
BfaI CTAG 1 cut(s) 1249
BfmI CTRYAG 1 cut(s) 66
BfrI CTTAAG 2 cut(s) 629, 1177
BglI GCCNNNNNGGC 1 cut(s) 228
BisI GCNGC 6 cut(s) 69, 94, 305, 308, 705, 1087
BlsI GCNGC 6 cut(s) 70, 95, 306, 309, 706, 1088
Bme1390I CCNGG 1 cut(s) 181
Bme18I GGWCC 1 cut(s) 183
BmgT120I GGNCC 2 cut(s) 183, 1097
BmiI GGNNCC 3 cut(s) 184, 232, 874
BmrFI CCNGG 1 cut(s) 181
BmsI GCATC 4 cut(s) 544, 676, 970, 1127
BpiI GAAGAC 1 cut(s) 379
BpuMI CCSGG 1 cut(s) 181
Bsa29I ATCGAT 1 cut(s) 199
BsaHI GRCGYC 1 cut(s) 99
BsaJI CCNNGG 1 cut(s) 123
BsaXI ACNNNNNCTCC 6 cut(s) 215, 245, 434, 464, 989, 1019
Bsc4I CCNNNNNNNGG 2 cut(s) 696, 1074
Bse118I RCCGGY 1 cut(s) 118
Bse1I ACTGG 2 cut(s) 210, 1100
Bse3DI GCAATG 1 cut(s) 781
BseCI ATCGAT 1 cut(s) 199
BseDI CCNNGG 1 cut(s) 123
BseGI GGATG 4 cut(s) 10, 264, 820, 853
BseLI CCNNNNNNNGG 2 cut(s) 696, 1074
BseMI GCAATG 1 cut(s) 781
BseMII CTCAG 4 cut(s) 42, 60, 283, 308
BseNI ACTGG 2 cut(s) 210, 1100
BseRI GAGGAG 2 cut(s) 544, 1241
BseXI GCAGC 5 cut(s) 80, 105, 316, 319, 691
Bsh1236I CGCG 1 cut(s) 433
BshFI GGCC 6 cut(s) 122, 222, 322, 1089, 1099, 1164
BshVI ATCGAT 1 cut(s) 199
BsiHKAI GWGCWC 2 cut(s) 440, 779
BsiSI CCGG 3 cut(s) 119, 180, 219
BslI CCNNNNNNNGG 2 cut(s) 696, 1074
BsmAI GTCTC 1 cut(s) 47
BsmBI CGTCTC 1 cut(s) 47
BsnI GGCC 6 cut(s) 122, 222, 322, 1089, 1099, 1164
Bsp1286I GDGCHC 2 cut(s) 440, 779
Bsp1407I TGTACA 1 cut(s) 905
Bsp143I GATC 8 cut(s) 138, 200, 313, 411, 611, 669, 919, 1013
BspACI CCGC 6 cut(s) 116, 255, 362, 433, 870, 1086
BspANI GGCC 6 cut(s) 122, 222, 322, 1089, 1099, 1164
BspCNI CTCAG 4 cut(s) 41, 59, 282, 309
BspDI ATCGAT 1 cut(s) 199
BspFNI CGCG 1 cut(s) 433
BspHI TCATGA 2 cut(s) 501, 679
BspLI GGNNCC 3 cut(s) 184, 232, 874
BspMAI CTGCAG 1 cut(s) 70
BspPI GGATC 3 cut(s) 419, 619, 1021
BspTI CTTAAG 2 cut(s) 629, 1177
BsrDI GCAATG 1 cut(s) 781
BsrFI RCCGGY 1 cut(s) 118
BsrGI TGTACA 1 cut(s) 905
BsrI ACTGG 2 cut(s) 210, 1100
BssAI RCCGGY 1 cut(s) 118
BssECI CCNNGG 1 cut(s) 123
BssMI GATC 8 cut(s) 138, 200, 313, 411, 611, 669, 919, 1013
BssNI GRCGYC 1 cut(s) 99
Bst4CI ACNGT 1 cut(s) 373
Bst6I CTCTTC 1 cut(s) 1037
BstACI GRCGYC 1 cut(s) 99
BstAFI CTTAAG 2 cut(s) 629, 1177
BstAUI TGTACA 1 cut(s) 905
BstC8I GCNNGC 3 cut(s) 116, 120, 1116
BstDEI CTNAG 4 cut(s) 28, 46, 269, 317
BstF5I GGATG 4 cut(s) 10, 264, 820, 853
BstFNI CGCG 1 cut(s) 433
BstHHI GCGC 3 cut(s) 169, 397, 961
BstKTI GATC 8 cut(s) 141, 203, 316, 414, 614, 672, 922, 1016
BstMAI GTCTC 1 cut(s) 47
BstMBI GATC 8 cut(s) 138, 200, 313, 411, 611, 669, 919, 1013
BstMWI GCNNNNNNNGC 4 cut(s) 77, 228, 783, 1120
BstNSI RCATGY 1 cut(s) 563
BstSCI CCNGG 1 cut(s) 179
BstSFI CTRYAG 1 cut(s) 66
BstUI CGCG 1 cut(s) 433
BstV1I GCAGC 5 cut(s) 80, 105, 316, 319, 691
BstV2I GAAGAC 1 cut(s) 379
BstX2I RGATCY 1 cut(s) 611
BstYI RGATCY 1 cut(s) 611
Bsu15I ATCGAT 1 cut(s) 199
BsuRI GGCC 6 cut(s) 122, 222, 322, 1089, 1099, 1164
BsuTUI ATCGAT 1 cut(s) 199
BtgZI GCGATG 2 cut(s) 360, 1104
BtsCI GGATG 4 cut(s) 10, 264, 820, 853
BtsI GCAGTG 1 cut(s) 978
BtsIMutI CAGTG 3 cut(s) 203, 277, 978
Cac8I GCNNGC 3 cut(s) 116, 120, 1116
CciI TCATGA 2 cut(s) 501, 679
CfoI GCGC 3 cut(s) 169, 397, 961
Cfr10I RCCGGY 1 cut(s) 118
Cfr13I GGNCC 2 cut(s) 183, 1097
ClaI ATCGAT 1 cut(s) 199
CseI GACGC 1 cut(s) 107
Csp6I GTAC 2 cut(s) 906, 1221
CviAII CATG 8 cut(s) 16, 159, 502, 560, 680, 1091, 1147, 1240
CviQI GTAC 2 cut(s) 906, 1221
DdeI CTNAG 4 cut(s) 28, 46, 269, 317
DpnI GATC 8 cut(s) 140, 202, 315, 413, 613, 671, 921, 1015
DpnII GATC 8 cut(s) 138, 200, 313, 411, 611, 669, 919, 1013
EaeI YGGCCR 2 cut(s) 220, 1087
Eam1104I CTCTTC 1 cut(s) 1037
EarI CTCTTC 1 cut(s) 1037
Ecl136II GAGCTC 2 cut(s) 438, 777
Eco147I AGGCCT 2 cut(s) 322, 1164
Eco24I GRGCYC 2 cut(s) 440, 779
Eco47I GGWCC 1 cut(s) 183
Eco53kI GAGCTC 2 cut(s) 438, 777
EcoICRI GAGCTC 2 cut(s) 438, 777
EcoO109I RGGNCCY 1 cut(s) 183
EcoT38I GRGCYC 2 cut(s) 440, 779
Esp3I CGTCTC 1 cut(s) 47
FaeI CATG 8 cut(s) 19, 162, 505, 563, 683, 1094, 1150, 1243
FalI AAGNNNNNCTT 2 cut(s) 782, 814
FatI CATG 8 cut(s) 15, 158, 501, 559, 679, 1090, 1146, 1239
FauI CCCGC 1 cut(s) 123
FbaI TGATCA 1 cut(s) 919
Fnu4HI GCNGC 6 cut(s) 69, 94, 305, 308, 705, 1087
FokI GGATG 3 cut(s) 251, 827, 860
FriOI GRGCYC 2 cut(s) 440, 779
Fsp4HI GCNGC 6 cut(s) 69, 94, 305, 308, 705, 1087
FspBI CTAG 1 cut(s) 1249
GlaI GCGC 3 cut(s) 168, 396, 960
GluI GCNGC 6 cut(s) 69, 94, 305, 308, 705, 1087
HaeIII GGCC 6 cut(s) 122, 222, 322, 1089, 1099, 1164
HapII CCGG 3 cut(s) 119, 180, 219
HgaI GACGC 1 cut(s) 107
HhaI GCGC 3 cut(s) 169, 397, 961
Hin1I GRCGYC 1 cut(s) 99
Hin1II CATG 8 cut(s) 19, 162, 505, 563, 683, 1094, 1150, 1243
Hin6I GCGC 3 cut(s) 167, 395, 959
HinP1I GCGC 3 cut(s) 167, 395, 959
HindIII AAGCTT 2 cut(s) 1050, 1061
HinfI GANTC 2 cut(s) 22, 665
HpaII CCGG 3 cut(s) 119, 180, 219
HphI GGTGA 2 cut(s) 367, 929
Hpy166II GTNNAC 2 cut(s) 604, 906
Hpy188I TCNGA 9 cut(s) 31, 106, 318, 338, 416, 442, 649, 844, 877
Hpy188III TCNNGA 5 cut(s) 136, 502, 609, 680, 1039
Hpy8I GTNNAC 2 cut(s) 604, 906
Hpy99I CGWCG 2 cut(s) 101, 110
HpyAV CCTTC 7 cut(s) 196, 352, 418, 761, 784, 893, 1088
HpyCH4III ACNGT 1 cut(s) 373
HpyCH4IV ACGT 2 cut(s) 40, 130
HpyCH4V TGCA 4 cut(s) 68, 162, 707, 1114
HpyF10VI GCNNNNNNNGC 4 cut(s) 77, 228, 783, 1120
HpyF3I CTNAG 4 cut(s) 28, 46, 269, 317
HpySE526I ACGT 2 cut(s) 40, 130
Hsp92I GRCGYC 1 cut(s) 99
Hsp92II CATG 8 cut(s) 19, 162, 505, 563, 683, 1094, 1150, 1243
HspAI GCGC 3 cut(s) 167, 395, 959
KroI GCCGGC 1 cut(s) 118
KroNI GCCGGC 1 cut(s) 120
Ksp22I TGATCA 1 cut(s) 919
Kzo9I GATC 8 cut(s) 138, 200, 313, 411, 611, 669, 919, 1013
LmnI GCTCC 2 cut(s) 236, 435
Lsp1109I GCAGC 5 cut(s) 80, 105, 316, 319, 691
LweI GCATC 4 cut(s) 544, 676, 970, 1127
MaeI CTAG 1 cut(s) 1249
MaeII ACGT 2 cut(s) 40, 130
MaeIII GTNAC 2 cut(s) 49, 367
MalI GATC 8 cut(s) 140, 202, 315, 413, 613, 671, 921, 1015
MboI GATC 8 cut(s) 138, 200, 313, 411, 611, 669, 919, 1013
MboII GAAGA 3 cut(s) 181, 379, 1054
MflI RGATCY 1 cut(s) 611
MhlI GDGCHC 2 cut(s) 440, 779
MluCI AATT 3 cut(s) 924, 1071, 1126
MlyI GAGTC 2 cut(s) 16, 674
MmeI TCCRAC 2 cut(s) 129, 914
MroNI GCCGGC 1 cut(s) 118
MseI TTAA 4 cut(s) 630, 693, 825, 1178
MslI CAYNNNNRTG 3 cut(s) 296, 684, 813
MspCI CTTAAG 2 cut(s) 629, 1177
MspI CCGG 3 cut(s) 119, 180, 219
MspR9I CCNGG 1 cut(s) 181
MvnI CGCG 1 cut(s) 433
MwoI GCNNNNNNNGC 4 cut(s) 77, 228, 783, 1120
NaeI GCCGGC 1 cut(s) 120
NciI CCSGG 1 cut(s) 181
NdeII GATC 8 cut(s) 138, 200, 313, 411, 611, 669, 919, 1013
NgoMIV GCCGGC 1 cut(s) 118
NlaIII CATG 8 cut(s) 19, 162, 505, 563, 683, 1094, 1150, 1243
NlaIV GGNNCC 3 cut(s) 184, 232, 874
NmeAIII GCCGAG 1 cut(s) 104
NmuCI GTSAC 2 cut(s) 49, 367
NspI RCATGY 1 cut(s) 563
PagI TCATGA 2 cut(s) 501, 679
PceI AGGCCT 2 cut(s) 322, 1164
PcsI WCGNNNNNNNCGW 2 cut(s) 105, 342
PdiI GCCGGC 1 cut(s) 120
PkrI GCNGC 6 cut(s) 70, 95, 306, 309, 706, 1088
PleI GAGTC 2 cut(s) 16, 673
PpsI GAGTC 2 cut(s) 16, 673
PpuMI RGGWCCY 1 cut(s) 183
PshBI ATTAAT 1 cut(s) 693
Psp124BI GAGCTC 2 cut(s) 440, 779
Psp5II RGGWCCY 1 cut(s) 183
PspN4I GGNNCC 3 cut(s) 184, 232, 874
PspPI GGNCC 2 cut(s) 183, 1097
PspPPI RGGWCCY 1 cut(s) 183
PstI CTGCAG 1 cut(s) 70
PsuI RGATCY 1 cut(s) 611
RsaI GTAC 2 cut(s) 907, 1222
RsaNI GTAC 2 cut(s) 906, 1221
RseI CAYNNNNRTG 3 cut(s) 296, 684, 813
SacI GAGCTC 2 cut(s) 440, 779
SaqAI TTAA 4 cut(s) 630, 693, 825, 1178
SatI GCNGC 6 cut(s) 69, 94, 305, 308, 705, 1087
Sau3AI GATC 8 cut(s) 138, 200, 313, 411, 611, 669, 919, 1013
Sau96I GGNCC 2 cut(s) 183, 1097
SchI GAGTC 2 cut(s) 16, 674
ScrFI CCNGG 1 cut(s) 181
SduI GDGCHC 2 cut(s) 440, 779
SfaNI GCATC 4 cut(s) 544, 676, 970, 1127
SfcI CTRYAG 1 cut(s) 66
SinI GGWCC 1 cut(s) 183
SmiMI CAYNNNNRTG 3 cut(s) 296, 684, 813
SmlI CTYRAG 2 cut(s) 629, 1177
SmoI CTYRAG 2 cut(s) 629, 1177
Sse9I AATT 3 cut(s) 924, 1071, 1126
SseBI AGGCCT 2 cut(s) 322, 1164
SsiI CCGC 6 cut(s) 116, 255, 362, 433, 870, 1086
SspMI CTAG 1 cut(s) 1249
SstI GAGCTC 2 cut(s) 440, 779
StuI AGGCCT 2 cut(s) 322, 1164
StyD4I CCNGG 1 cut(s) 179
TaaI ACNGT 1 cut(s) 373
TaiI ACGT 2 cut(s) 43, 133
TaqI TCGA 5 cut(s) 135, 199, 462, 668, 980
TasI AATT 3 cut(s) 924, 1071, 1126
TatI WGTACW 2 cut(s) 905, 1220
TauI GCSGC 1 cut(s) 1089
Tru1I TTAA 4 cut(s) 630, 693, 825, 1178
Tru9I TTAA 4 cut(s) 630, 693, 825, 1178
TscAI CASTG 3 cut(s) 210, 277, 978
TseFI GTSAC 2 cut(s) 49, 367
TseI GCWGC 5 cut(s) 68, 93, 304, 307, 704
Tsp45I GTSAC 2 cut(s) 49, 367
TspDTI ATGAA 5 cut(s) 253, 490, 518, 1107, 1135
TspRI CASTG 3 cut(s) 210, 277, 978
Vha464I CTTAAG 2 cut(s) 629, 1177
VpaK11BI GGWCC 1 cut(s) 183
VspI ATTAAT 1 cut(s) 693
XceI RCATGY 1 cut(s) 563
XcmI CCANNNNNNNNNTGG 1 cut(s) 697
XspI CTAG 1 cut(s) 1249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.