MD07G1027800.v1.1

Myosin heavy chain kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0014503
Physical Location & Seq
Reverse (-)
2322648 .. 2324054
1407 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1027800.v1.1.491

Sequence Viewer

Length: 1407 bp
ATGTATATGTCCCTAATCTTTCCACCAAGAAATCCGTGTTCCCTAATCCGCTCCTCTCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTGTGTGACCATGTTTCATCCAATGAATTCCACTCTGTTAGAAAACAAACGTCTCTCAACAACTCCCTTGCTCTCTGCCTCAGCAACAAGCACCAGCAGCAGCACGAGCAGCGAGGCCGATGGCAGCCCGCCGGATTCAGGCCGCTTTTCGCTCCAAGATCATGTCAAGTATCAATTCCCCTGCGTACCCTTATCCGGGTCCTTCTCGTATCGATCGTTGGCGGTGCTCCCCGGACATGTAGGCTCGGTTTCGTGCTTGGCCTTGTGTGGTGAATTCATCCTCAGCGCCTCACAAGGCAAAGACATCATAGTCTGGCAACAGCCGGATTTAAGGCCTTTCACGAAATTCGGACAAGGCGATGGCAGCGTCAAGGCGGTTGTTGCTGTCGGGAACAAGGTTTTTACAGCGCACCAGGACAGCAGGATCAGGGTTTGGAAGGTTTCGCGGAGGTCGGAGAATGTCTTTAGGCTTGTCGACACGCTTCCCACAACGAAAGACTATTTGGGGAAGTTCATGAAGCAGAGTAACTATGTCCAAACTCGGAGACATCACAAGCGACTGTGGATCGAACACGCGGATAGTATATCGTGTTTGGCTTTCCATAACGAGTTGATTTACTCTGGTTCATGGGACAAGACGCTCAAGGTGTGGAGGATCTCGGATTTGAAGTGCGTAGAGTCGATTAAGGCTCACGACGACGCAATCAACGGCTTGGTGGCAAGTAAAGGGACTGTGTATTCTGCTTCTGCAGATGGGAAAATCAAAGCTTGGGGAAGAGAGGGGAAGAAGAAGAGCACACATTGTTTGAAGGGTATTCTGGAGGGGCACAAGGATGTTTCGCTTAACTCCGTGATCGTTTCAGAGGATGGGAGATGGGTTTATGGGGGCGGCTCTGACGGGTTTGTGATGGGATGGGAAGCGAGTAATTGCAGTACTAATGGTGGTGATGTTCAAAACAAACTTGTGGTAAGTTGGAAGCTGGTTTGCGAAACAAAAGCGCATGGGATGGCGGTTTTGTCGATGTGTTTGATGGGGGAGATGCTGTGCAGCGGCTCGGCGGATAAGAGCATCGGCATATGGAAGAGGGAGGCTTTCGGTAAGCTATGTAAAGTTGGGGTAATTAGCGGCCATGAAGGACCAGTGAAGTGCTTGCAGGCAGCACCAAATAACGTCGGTGGCGGATTCATGTTGTACAGTGGAGGACTTGATAAGAGATTGAGAGTTTGGTGGGTTGCAAAACCTTCTAAAACCAGAGAGGAAGATACCAATTCAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

469

Amino Acids

50.97

Weight (kDa)

9.23

Isoelectric Point (pI)

38.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 119 - 317 8.2e-17 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 171 - 323 1.3e-06 WDHD1 first WD40 domain
WDR55 PF24796 225 - 429 2.6e-06 WDR55
Beta-prop_WDR3_1st PF25173 232 - 349 1.8e-10 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 233 - 349 4.8e-08 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 233 - 354 2.2e-07 CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd PF25047 234 - 307 3.9e-07 TEP-1 second beta-propeller
WD40_Prp19 PF24814 237 - 452 1.1e-13 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 243 - 352 2.8e-09 WDR3 second beta-propeller domain
Beta-prop_WDR5 PF25175 316 - 456 9e-09 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 369 - 458 1.3e-06 TEP-1 second beta-propeller
Beta-prop_THOC3 PF25174 386 - 462 8.6e-07 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014503)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51930
fragaria_vesca FvH4_7g02030
malus_domestica MD02G1296500.v1.1 MD07G1027800.v1.1
prunus_persica Prupe.2G027900_v2.0.a1 Prupe.2G027900_v2.0.a1
pyrus_communis pycom02g24950 pycom07g02110
rosa_chinensis RchiOBHm_Chr1g0319521
rosa_laevigata RLG00000030475
rosa_multiflora Rmu_sc0002539.1_g000012
rosa_roxburghii Rroxscaffold_4G00328310
rosa_samantha Rh1AG040100 Rh1BG037000 Rh1CG041100 Rh1DG028800
rosa_wichuraiana Rw1G003240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 437
AccBSI CCGCTC 1 cut(s) 51
AccI GTMKAC 1 cut(s) 603
AccII CGCG 2 cut(s) 574, 704
AclWI GGATC 3 cut(s) 560, 701, 791
AcoI YGGCCR 1 cut(s) 1255
AcsI RAATTY 3 cut(s) 154, 401, 473
AfaI GTAC 3 cut(s) 315, 1063, 1322
AfiI CCNNNNNNNGG 3 cut(s) 266, 323, 324
AflIII ACRYGT 1 cut(s) 364
AgsI TTSAA 3 cut(s) 796, 937, 1082
AjnI CCWGG 1 cut(s) 540
AjuI GAANNNNNNNTTGG 2 cut(s) 614, 646
AluBI AGCT 3 cut(s) 896, 1108, 1231
AluI AGCT 3 cut(s) 896, 1108, 1231
Alw21I GWGCWC 2 cut(s) 357, 926
Alw26I GTCTC 2 cut(s) 185, 667
AlwI GGATC 3 cut(s) 560, 701, 791
AoxI GGCC 5 cut(s) 243, 268, 387, 461, 1255
ApeKI GCWGC 7 cut(s) 225, 228, 237, 252, 492, 1176, 1286
ApoI RAATTY 3 cut(s) 154, 401, 473
AspLEI GCGC 3 cut(s) 416, 538, 1129
AspS9I GGNCC 2 cut(s) 327, 1265
AsuC2I CCSGG 2 cut(s) 325, 360
AsuHPI GGTGA 2 cut(s) 410, 1085
AvaII GGWCC 2 cut(s) 327, 1265
BaeGI GKGCMC 1 cut(s) 957
BauI CACGAG 1 cut(s) 232
Bbv12I GWGCWC 2 cut(s) 357, 926
BbvCI CCTCAGC 2 cut(s) 208, 410
BbvI GCAGC 7 cut(s) 237, 240, 249, 264, 504, 1188, 1298
BccI CCATC 9 cut(s) 242, 482, 875, 989, 996, 1030, 1035, 1129, 1153
BceAI ACGGC 1 cut(s) 853
BciT130I CCWGG 1 cut(s) 542
BcnI CCSGG 2 cut(s) 325, 360
BcoDI GTCTC 2 cut(s) 185, 667
BfmI CTRYAG 1 cut(s) 876
BfoI RGCGCY 1 cut(s) 417
BmcAI AGTACT 1 cut(s) 1063
Bme1390I CCNGG 3 cut(s) 325, 360, 542
Bme18I GGWCC 2 cut(s) 327, 1265
BmgT120I GGNCC 2 cut(s) 327, 1265
BmiI GGNNCC 1 cut(s) 328
BmrFI CCNGG 3 cut(s) 325, 360, 542
BmsI GCATC 2 cut(s) 1158, 1206
BpmI CTGGAG 1 cut(s) 968
Bpu10I CCTNAGC 2 cut(s) 208, 410
BpuEI CTTGAG 1 cut(s) 755
BpuMI CCSGG 2 cut(s) 325, 360
Bsa29I ATCGAT 1 cut(s) 340
BsaJI CCNNGG 1 cut(s) 358
BsaXI ACNNNNNCTCC 2 cut(s) 1157, 1187
Bsc4I CCNNNNNNNGG 3 cut(s) 266, 323, 324
Bse1I ACTGG 1 cut(s) 1268
BseBI CCWGG 1 cut(s) 542
BseCI ATCGAT 1 cut(s) 340
BseDI CCNNGG 1 cut(s) 358
BseGI GGATG 6 cut(s) 145, 405, 967, 1000, 1046, 1140
BseLI CCNNNNNNNGG 3 cut(s) 266, 323, 324
BseMII CTCAG 2 cut(s) 222, 424
BseNI ACTGG 1 cut(s) 1268
BseRI GAGGAG 2 cut(s) 43, 48
BseSI GKGCMC 1 cut(s) 957
BseXI GCAGC 7 cut(s) 237, 240, 249, 264, 504, 1188, 1298
BsgI GTGCAG 1 cut(s) 1195
Bsh1236I CGCG 2 cut(s) 574, 704
Bsh1285I CGRYCG 1 cut(s) 344
BshFI GGCC 5 cut(s) 245, 270, 389, 463, 1257
BshVI ATCGAT 1 cut(s) 340
BsiEI CGRYCG 1 cut(s) 344
BsiHKAI GWGCWC 2 cut(s) 357, 926
BsiSI CCGG 4 cut(s) 260, 324, 360, 452
BslFI GGGAC 2 cut(s) 773, 871
BslI CCNNNNNNNGG 3 cut(s) 266, 323, 324
BsmAI GTCTC 2 cut(s) 185, 667
BsmBI CGTCTC 1 cut(s) 185
BsmFI GGGAC 2 cut(s) 773, 871
BsnI GGCC 5 cut(s) 245, 270, 389, 463, 1257
Bsp1286I GDGCHC 3 cut(s) 357, 926, 957
Bsp1407I TGTACA 1 cut(s) 1320
Bsp143I GATC 6 cut(s) 286, 341, 552, 693, 783, 981
BspANI GGCC 5 cut(s) 245, 270, 389, 463, 1257
BspCNI CTCAG 2 cut(s) 221, 423
BspDI ATCGAT 1 cut(s) 340
BspFNI CGCG 2 cut(s) 574, 704
BspHI TCATGA 1 cut(s) 642
BspLI GGNNCC 1 cut(s) 328
BspMAI CTGCAG 1 cut(s) 880
BspPI GGATC 3 cut(s) 560, 701, 791
BspQI GCTCTTC 1 cut(s) 914
BsrBI CCGCTC 1 cut(s) 51
BsrGI TGTACA 1 cut(s) 1320
BsrI ACTGG 1 cut(s) 1268
BssECI CCNNGG 1 cut(s) 358
BssMI GATC 6 cut(s) 286, 341, 552, 693, 783, 981
BssSI CACGAG 1 cut(s) 232
Bst2BI CACGAG 1 cut(s) 232
Bst2UI CCWGG 1 cut(s) 542
Bst4CI ACNGT 3 cut(s) 690, 862, 1325
Bst6I CTCTTC 3 cut(s) 898, 914, 1205
BstAUI TGTACA 1 cut(s) 1320
BstC8I GCNNGC 3 cut(s) 257, 1280, 1284
BstDEI CTNAG 2 cut(s) 208, 410
BstF5I GGATG 6 cut(s) 145, 405, 967, 1000, 1046, 1140
BstFNI CGCG 2 cut(s) 574, 704
BstH2I RGCGCY 1 cut(s) 417
BstHHI GCGC 3 cut(s) 416, 538, 1129
BstKTI GATC 6 cut(s) 289, 344, 555, 696, 786, 984
BstMAI GTCTC 2 cut(s) 185, 667
BstMBI GATC 6 cut(s) 286, 341, 552, 693, 783, 981
BstMCI CGRYCG 1 cut(s) 344
BstMWI GCNNNNNNNGC 5 cut(s) 225, 234, 237, 492, 509
BstNI CCWGG 1 cut(s) 542
BstNSI RCATGY 1 cut(s) 368
BstSCI CCNGG 3 cut(s) 323, 358, 540
BstSFI CTRYAG 1 cut(s) 876
BstSLI GKGCMC 1 cut(s) 957
BstUI CGCG 2 cut(s) 574, 704
BstV1I GCAGC 7 cut(s) 237, 240, 249, 264, 504, 1188, 1298
BstX2I RGATCY 1 cut(s) 783
BstYI RGATCY 1 cut(s) 783
Bsu15I ATCGAT 1 cut(s) 340
BsuRI GGCC 5 cut(s) 245, 270, 389, 463, 1257
BsuTUI ATCGAT 1 cut(s) 340
BtgZI GCGATG 1 cut(s) 501
BtsCI GGATG 6 cut(s) 145, 405, 967, 1000, 1046, 1140
BtsIMutI CAGTG 2 cut(s) 1275, 1330
Cac8I GCNNGC 3 cut(s) 257, 1280, 1284
CciI TCATGA 1 cut(s) 642
CfoI GCGC 3 cut(s) 416, 538, 1129
Cfr13I GGNCC 2 cut(s) 327, 1265
ClaI ATCGAT 1 cut(s) 340
CseI GACGC 3 cut(s) 484, 775, 836
Csp6I GTAC 3 cut(s) 314, 1062, 1321
CviAII CATG 8 cut(s) 139, 290, 365, 643, 756, 1130, 1259, 1315
CviQI GTAC 3 cut(s) 314, 1062, 1321
DdeI CTNAG 2 cut(s) 208, 410
DpnI GATC 6 cut(s) 288, 343, 554, 695, 785, 983
DpnII GATC 6 cut(s) 286, 341, 552, 693, 783, 981
DrdI GACNNNNNNGTC 1 cut(s) 437
DseDI GACNNNNNNGTC 1 cut(s) 437
EaeI YGGCCR 1 cut(s) 1255
Eam1104I CTCTTC 3 cut(s) 898, 914, 1205
EarI CTCTTC 3 cut(s) 898, 914, 1205
EciI GGCGGA 2 cut(s) 1202, 1323
Eco147I AGGCCT 1 cut(s) 463
Eco47I GGWCC 2 cut(s) 327, 1265
EcoO109I RGGNCCY 1 cut(s) 327
EcoRI GAATTC 2 cut(s) 154, 401
EcoRII CCWGG 1 cut(s) 540
Esp3I CGTCTC 1 cut(s) 185
FaeI CATG 8 cut(s) 142, 293, 368, 646, 759, 1133, 1262, 1318
FaqI GGGAC 2 cut(s) 773, 871
FatI CATG 8 cut(s) 138, 289, 364, 642, 755, 1129, 1258, 1314
FauI CCCGC 1 cut(s) 264
FauNDI CATATG 1 cut(s) 1205
FblI GTMKAC 1 cut(s) 603
FokI GGATG 6 cut(s) 132, 392, 974, 1007, 1053, 1147
GlaI GCGC 3 cut(s) 415, 537, 1128
GsuI CTGGAG 1 cut(s) 968
HaeII RGCGCY 1 cut(s) 417
HaeIII GGCC 5 cut(s) 245, 270, 389, 463, 1257
HapII CCGG 4 cut(s) 260, 324, 360, 452
HgaI GACGC 3 cut(s) 484, 775, 836
HhaI GCGC 3 cut(s) 416, 538, 1129
Hin1II CATG 8 cut(s) 142, 293, 368, 646, 759, 1133, 1262, 1318
Hin6I GCGC 3 cut(s) 414, 536, 1127
HinP1I GCGC 3 cut(s) 414, 536, 1127
HincII GTYRAC 1 cut(s) 604
HindII GTYRAC 1 cut(s) 604
HindIII AAGCTT 1 cut(s) 894
HinfI GANTC 3 cut(s) 263, 806, 1311
HpaII CCGG 4 cut(s) 260, 324, 360, 452
HphI GGTGA 2 cut(s) 410, 1085
Hpy166II GTNNAC 1 cut(s) 604
Hpy188I TCNGA 6 cut(s) 479, 583, 672, 790, 991, 1024
Hpy188III TCNNGA 5 cut(s) 469, 517, 643, 821, 947
Hpy8I GTNNAC 1 cut(s) 604
Hpy99I CGWCG 3 cut(s) 827, 830, 1304
HpyAV CCTTC 5 cut(s) 340, 559, 931, 1256, 1380
HpyCH4III ACNGT 3 cut(s) 690, 862, 1325
HpyCH4IV ACGT 2 cut(s) 178, 1299
HpyCH4V TGCA 5 cut(s) 878, 1059, 1176, 1282, 1364
HpyF10VI GCNNNNNNNGC 5 cut(s) 225, 234, 237, 492, 509
HpyF3I CTNAG 2 cut(s) 208, 410
HpySE526I ACGT 2 cut(s) 178, 1299
Hsp92II CATG 8 cut(s) 142, 293, 368, 646, 759, 1133, 1262, 1318
HspAI GCGC 3 cut(s) 414, 536, 1127
Kzo9I GATC 6 cut(s) 286, 341, 552, 693, 783, 981
LguI GCTCTTC 1 cut(s) 914
LmnI GCTCC 3 cut(s) 56, 285, 360
Lsp1109I GCAGC 7 cut(s) 237, 240, 249, 264, 504, 1188, 1298
LweI GCATC 2 cut(s) 1158, 1206
MaeII ACGT 2 cut(s) 178, 1299
MaeIII GTNAC 2 cut(s) 133, 653
MalI GATC 6 cut(s) 288, 343, 554, 695, 785, 983
MbiI CCGCTC 1 cut(s) 51
MboI GATC 6 cut(s) 286, 341, 552, 693, 783, 981
MboII GAAGA 6 cut(s) 915, 925, 928, 931, 1222, 1400
MflI RGATCY 1 cut(s) 783
MhlI GDGCHC 3 cut(s) 357, 926, 957
MluCI AATT 7 cut(s) 154, 302, 401, 473, 1054, 1248, 1396
MlyI GAGTC 1 cut(s) 815
MmeI TCCRAC 2 cut(s) 561, 1082
MseI TTAA 3 cut(s) 458, 813, 972
MslI CAYNNNNRTG 1 cut(s) 960
MspA1I CMGCKG 1 cut(s) 1179
MspI CCGG 4 cut(s) 260, 324, 360, 452
MspR9I CCNGG 3 cut(s) 325, 360, 542
MvaI CCWGG 1 cut(s) 542
MvnI CGCG 2 cut(s) 574, 704
MwoI GCNNNNNNNGC 5 cut(s) 225, 234, 237, 492, 509
NciI CCSGG 2 cut(s) 325, 360
NdeI CATATG 1 cut(s) 1205
NdeII GATC 6 cut(s) 286, 341, 552, 693, 783, 981
NlaIII CATG 8 cut(s) 142, 293, 368, 646, 759, 1133, 1262, 1318
NlaIV GGNNCC 1 cut(s) 328
NmeAIII GCCGAG 1 cut(s) 1163
NmuCI GTSAC 1 cut(s) 133
NspI RCATGY 1 cut(s) 368
PagI TCATGA 1 cut(s) 642
PceI AGGCCT 1 cut(s) 463
PciI ACATGT 1 cut(s) 364
PciSI GCTCTTC 1 cut(s) 914
PcsI WCGNNNNNNNCGW 2 cut(s) 341, 483
PfeI GAWTC 2 cut(s) 263, 1311
Ple19I CGATCG 1 cut(s) 344
PleI GAGTC 1 cut(s) 814
PpsI GAGTC 1 cut(s) 814
PpuMI RGGWCCY 1 cut(s) 327
PscI ACATGT 1 cut(s) 364
Psp5II RGGWCCY 1 cut(s) 327
Psp6I CCWGG 1 cut(s) 540
PspGI CCWGG 1 cut(s) 540
PspN4I GGNNCC 1 cut(s) 328
PspPI GGNCC 2 cut(s) 327, 1265
PspPPI RGGWCCY 1 cut(s) 327
PstI CTGCAG 1 cut(s) 880
PsuI RGATCY 1 cut(s) 783
PvuI CGATCG 1 cut(s) 344
RsaI GTAC 3 cut(s) 315, 1063, 1322
RsaNI GTAC 3 cut(s) 314, 1062, 1321
RseI CAYNNNNRTG 1 cut(s) 960
SalI GTCGAC 1 cut(s) 602
SapI GCTCTTC 1 cut(s) 914
SaqAI TTAA 3 cut(s) 458, 813, 972
Sau3AI GATC 6 cut(s) 286, 341, 552, 693, 783, 981
Sau96I GGNCC 2 cut(s) 327, 1265
ScaI AGTACT 1 cut(s) 1063
SchI GAGTC 1 cut(s) 815
ScrFI CCNGG 3 cut(s) 325, 360, 542
SduI GDGCHC 3 cut(s) 357, 926, 957
SfaNI GCATC 2 cut(s) 1158, 1206
SfcI CTRYAG 1 cut(s) 876
SinI GGWCC 2 cut(s) 327, 1265
SmiMI CAYNNNNRTG 1 cut(s) 960
SmlI CTYRAG 1 cut(s) 770
SmoI CTYRAG 1 cut(s) 770
Sse9I AATT 7 cut(s) 154, 302, 401, 473, 1054, 1248, 1396
SseBI AGGCCT 1 cut(s) 463
StuI AGGCCT 1 cut(s) 463
StyD4I CCNGG 3 cut(s) 323, 358, 540
TaaI ACNGT 3 cut(s) 690, 862, 1325
TaiI ACGT 2 cut(s) 181, 1302
TaqI TCGA 5 cut(s) 340, 603, 696, 809, 1148
TasI AATT 7 cut(s) 154, 302, 401, 473, 1054, 1248, 1396
TatI WGTACW 2 cut(s) 1061, 1320
TauI GCSGC 4 cut(s) 273, 1020, 1182, 1257
TfiI GAWTC 2 cut(s) 263, 1311
Tru1I TTAA 3 cut(s) 458, 813, 972
Tru9I TTAA 3 cut(s) 458, 813, 972
TscAI CASTG 2 cut(s) 1275, 1330
TseFI GTSAC 1 cut(s) 133
TseI GCWGC 7 cut(s) 225, 228, 237, 252, 492, 1176, 1286
Tsp45I GTSAC 1 cut(s) 133
TspDTI ATGAA 8 cut(s) 134, 167, 394, 631, 659, 744, 1275, 1303
TspGWI ACGGA 2 cut(s) 24, 967
TspRI CASTG 2 cut(s) 1275, 1330
VpaK11BI GGWCC 2 cut(s) 327, 1265
XapI RAATTY 3 cut(s) 154, 401, 473
XceI RCATGY 1 cut(s) 368
XmiI GTMKAC 1 cut(s) 603
ZrmI AGTACT 1 cut(s) 1063
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.