Rw1G003240

Myosin heavy chain kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0014503
Physical Location & Seq
Reverse (-)
5959333 .. 5960982
1650 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G003240.1

Sequence Viewer

Length: 1284 bp
ATGTTTCATCCCATGACACTTTCAGAAAACAAACGCCTCTCAGTTACTCCCTTACTCTCTGCCACAGCAGCAAGCACCAGCAGCATCAGCAGCACAAGCAGCGAGGCCTCCGAAGAAAGCCCGCCGACCTCAGCACGTTTCGAGCTCCAGGATATCAAGTACCATCATTTTCCATGCACGCACTTATCCGGGTCCTTCTCCTACCGATCACTGGCTGTGCTTGCCGGCCATAAAGGCTCGATTTCGTGCCTGTCCTTATGTGGAGAGTTCATTCTCAGTGCTTCACAAGGCAAAGACATCATCGTGTGGCAGCAGCCTGATTTGAGGCCTTTCACCAAGTTCGGACAAGGCGATGGCTCGGTGAAGGCGGTTGTGACCGTTGGGAACAAGGTCTTTACGGCGCATCAAGACAGCAGGATCAGAGTTTGGAAGGTTTCGCGGAGTTCAGAGAATGTGTTTAAGCTTGTGGACACACTGCCTACAACGAAAGACTATTTGGGGAAGTTCATGAAGCAAAGCAATTATGTGCAAACGAGGAGGCACCACAAGAAGTTGTGGATTGAACATGCTGATAGTATCTCTTGTCTGGCTTTCTATAATGGGTTGATTTACTCTGGATCGTGGGATAAGACCCTTAAGGTGTGGAGAGTTTCGGATTTGAAGTGCGTGGAGTCGATTAAGGCTCATGATGATGCCATTAATGGGTTGGTGGCAAGTAAAGGGATCGTGTATTCTGCTTCTGCCGATGGAAAGATCAAGGCTTGGGGAAAAGAGGGGAAGAGCTCCCATTGCTTGAAGGGTATTTTGGAGGGTCATAAGGATGTTTCGTTCAATTCGGTGATTGTTTCAGAGGATGGGAGATGGGTTTATGGAGGCGGTTCCGATGGGTTTGTGATGGGATGGGAAGTGTGTACTAGTGGTGGTGATCAAGTTGCGAGCTGGAAGTTGGTTTCCGAAACAAAAGCGCATCAAATGGCAGTGTTGTCGATGTGTTTGATGGGGGAGATGTTGTGTAGTGGATCAGCAGATAAGAGTATTGGTATATGGAAGAGAGAGGCTTTTGGGAAGCTTTCCAAAATTGGGGTTATTAGTGGCCATGAAGGGCCAGTCAAGTGCTTGCAAGCATCGCCGAATAGTGTTGGTGGTGGTTTTATGTTGTATAGTGGAGGCCTTGACAAGACCTTAAGAGTTTGGTGGGTTTCCAAGCCCTCCACAACACAGAAGAGTACAGAGGAAAAGTCCATCATCTTGAGCGAGAAATTCTACAAAGTCTCTGTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

427

Amino Acids

46.67

Weight (kDa)

9.1

Isoelectric Point (pI)

39.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_SCAP PF24017 68 - 255 3.2e-07 SCAP Beta-propeller
Beta-prop_WDR5 PF25175 74 - 276 6.2e-18 WDR5 beta-propeller domain
WDR55 PF24796 175 - 375 6.7e-09 WDR55
Beta-prop_TEP1_2nd PF25047 186 - 260 3.1e-06 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 187 - 303 3.9e-11 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 188 - 327 6.9e-07 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 192 - 410 3.4e-17 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 200 - 302 2.2e-08 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 201 - 306 8.8e-08 THOC3 beta-propeller domain
Beta-prop_EML PF23409 252 - 376 2.9e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR5 PF25175 270 - 408 9.6e-11 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 290 - 405 4.6e-11 TEP-1 second beta-propeller
WD40_CDC20-Fz PF24807 314 - 416 3.2e-07 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 315 - 418 3.9e-07 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 328 - 414 1.5e-08 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014503)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51930
fragaria_vesca FvH4_7g02030
malus_domestica MD02G1296500.v1.1 MD07G1027800.v1.1
prunus_persica Prupe.2G027900_v2.0.a1 Prupe.2G027900_v2.0.a1
pyrus_communis pycom02g24950 pycom07g02110
rosa_chinensis RchiOBHm_Chr1g0319521
rosa_laevigata RLG00000030475
rosa_multiflora Rmu_sc0002539.1_g000012
rosa_roxburghii Rroxscaffold_4G00328310
rosa_samantha Rh1AG040100 Rh1BG037000 Rh1CG041100 Rh1DG028800
rosa_wichuraiana Rw1G003240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 540
AccII CGCG 1 cut(s) 439
AciI CCGC 4 cut(s) 122, 368, 439, 876
AclWI GGATC 4 cut(s) 425, 625, 731, 1027
AcoI YGGCCR 2 cut(s) 226, 1093
AcsI RAATTY 1 cut(s) 1259
AfaI GTAC 3 cut(s) 161, 913, 1228
AfiI CCNNNNNNNGG 3 cut(s) 211, 702, 1080
AflII CTTAAG 2 cut(s) 635, 1183
AgsI TTSAA 4 cut(s) 563, 661, 796, 832
AhlI ACTAGT 1 cut(s) 914
AjnI CCWGG 1 cut(s) 147
AjuI GAANNNNNNNTTGG 4 cut(s) 479, 511, 788, 820
AloI GAACNNNNNNTCC 2 cut(s) 812, 844
AluBI AGCT 5 cut(s) 145, 463, 783, 939, 1069
AluI AGCT 5 cut(s) 145, 463, 783, 939, 1069
Alw21I GWGCWC 2 cut(s) 147, 785
Alw26I GTCTC 1 cut(s) 1276
AlwI GGATC 4 cut(s) 425, 625, 731, 1027
AoxI GGCC 6 cut(s) 105, 226, 326, 1093, 1103, 1168
ApeKI GCWGC 6 cut(s) 68, 81, 90, 99, 310, 313
ApoI RAATTY 1 cut(s) 1259
AseI ATTAAT 1 cut(s) 699
AspLEI GCGC 2 cut(s) 403, 967
AspS9I GGNCC 2 cut(s) 192, 1103
AsuC2I CCSGG 1 cut(s) 190
AsuHPI GGTGA 4 cut(s) 325, 373, 850, 935
AvaII GGWCC 1 cut(s) 192
BalI TGGCCA 1 cut(s) 1095
BanI GGYRCC 1 cut(s) 540
BanII GRGCYC 2 cut(s) 147, 785
Bbv12I GWGCWC 2 cut(s) 147, 785
BbvCI CCTCAGC 1 cut(s) 130
BbvI GCAGC 6 cut(s) 80, 93, 102, 111, 322, 325
BceAI ACGGC 1 cut(s) 414
BcgI CGANNNNNNTGC 2 cut(s) 966, 1000
BciT130I CCWGG 1 cut(s) 149
BclI TGATCA 1 cut(s) 925
BcnI CCSGG 1 cut(s) 190
BcoDI GTCTC 1 cut(s) 1276
BcuI ACTAGT 1 cut(s) 914
BfaI CTAG 1 cut(s) 915
BfrI CTTAAG 2 cut(s) 635, 1183
BglI GCCNNNNNGGC 1 cut(s) 234
BisI GCNGC 6 cut(s) 69, 82, 91, 100, 311, 314
BlsI GCNGC 6 cut(s) 70, 83, 92, 101, 312, 315
Bme1390I CCNGG 2 cut(s) 149, 190
Bme18I GGWCC 1 cut(s) 192
BmgT120I GGNCC 2 cut(s) 192, 1103
BmiI GGNNCC 3 cut(s) 193, 542, 880
BmrFI CCNGG 2 cut(s) 149, 190
BmsI GCATC 5 cut(s) 93, 412, 682, 976, 1133
BpmI CTGGAG 1 cut(s) 131
Bpu10I CCTNAGC 1 cut(s) 130
BpuEI CTTGAG 1 cut(s) 1270
BpuMI CCSGG 1 cut(s) 190
BsaXI ACNNNNNCTCC 2 cut(s) 995, 1025
Bsc4I CCNNNNNNNGG 3 cut(s) 211, 702, 1080
Bse118I RCCGGY 1 cut(s) 224
Bse1I ACTGG 2 cut(s) 216, 1106
Bse3DI GCAATG 1 cut(s) 787
BseBI CCWGG 1 cut(s) 149
BseGI GGATG 4 cut(s) 7, 826, 859, 905
BseLI CCNNNNNNNGG 3 cut(s) 211, 702, 1080
BseMI GCAATG 1 cut(s) 787
BseMII CTCAG 3 cut(s) 54, 144, 289
BseNI ACTGG 2 cut(s) 216, 1106
BseRI GAGGAG 1 cut(s) 550
BseXI GCAGC 6 cut(s) 80, 93, 102, 111, 322, 325
Bsh1236I CGCG 1 cut(s) 439
BshFI GGCC 6 cut(s) 107, 228, 328, 1095, 1105, 1170
BshNI GGYRCC 1 cut(s) 540
BsiHKAI GWGCWC 2 cut(s) 147, 785
BsiSI CCGG 2 cut(s) 189, 225
BslI CCNNNNNNNGG 3 cut(s) 211, 702, 1080
BsmAI GTCTC 1 cut(s) 1276
BsnI GGCC 6 cut(s) 107, 228, 328, 1095, 1105, 1170
Bsp1286I GDGCHC 2 cut(s) 147, 785
Bsp143I GATC 7 cut(s) 206, 417, 617, 723, 753, 925, 1019
BspACI CCGC 4 cut(s) 122, 368, 439, 876
BspANI GGCC 6 cut(s) 107, 228, 328, 1095, 1105, 1170
BspCNI CTCAG 3 cut(s) 53, 143, 288
BspFNI CGCG 1 cut(s) 439
BspHI TCATGA 2 cut(s) 507, 685
BspLI GGNNCC 3 cut(s) 193, 542, 880
BspPI GGATC 4 cut(s) 425, 625, 731, 1027
BspQI GCTCTTC 1 cut(s) 773
BspT107I GGYRCC 1 cut(s) 540
BspTI CTTAAG 2 cut(s) 635, 1183
BsrDI GCAATG 1 cut(s) 787
BsrFI RCCGGY 1 cut(s) 224
BsrI ACTGG 2 cut(s) 216, 1106
BssAI RCCGGY 1 cut(s) 224
BssMI GATC 7 cut(s) 206, 417, 617, 723, 753, 925, 1019
Bst2UI CCWGG 1 cut(s) 149
Bst4CI ACNGT 1 cut(s) 379
Bst6I CTCTTC 3 cut(s) 773, 1043, 1217
BstAFI CTTAAG 2 cut(s) 635, 1183
BstC8I GCNNGC 8 cut(s) 73, 122, 179, 222, 226, 937, 1118, 1122
BstDEI CTNAG 3 cut(s) 40, 130, 275
BstF5I GGATG 4 cut(s) 7, 826, 859, 905
BstFNI CGCG 1 cut(s) 439
BstHHI GCGC 2 cut(s) 403, 967
BstKTI GATC 7 cut(s) 209, 420, 620, 726, 756, 928, 1022
BstMAI GTCTC 1 cut(s) 1276
BstMBI GATC 7 cut(s) 206, 417, 617, 723, 753, 925, 1019
BstNI CCWGG 1 cut(s) 149
BstNSI RCATGY 1 cut(s) 569
BstSCI CCNGG 2 cut(s) 147, 188
BstUI CGCG 1 cut(s) 439
BstV1I GCAGC 6 cut(s) 80, 93, 102, 111, 322, 325
BsuRI GGCC 6 cut(s) 107, 228, 328, 1095, 1105, 1170
BtgZI GCGATG 2 cut(s) 366, 1110
BtsCI GGATG 4 cut(s) 7, 826, 859, 905
BtsI GCAGTG 2 cut(s) 473, 984
BtsIMutI CAGTG 4 cut(s) 209, 283, 473, 984
Cac8I GCNNGC 8 cut(s) 73, 122, 179, 222, 226, 937, 1118, 1122
CciI TCATGA 2 cut(s) 507, 685
CfoI GCGC 2 cut(s) 403, 967
Cfr10I RCCGGY 1 cut(s) 224
Cfr13I GGNCC 2 cut(s) 192, 1103
Csp6I GTAC 3 cut(s) 160, 912, 1227
CviAII CATG 6 cut(s) 13, 174, 508, 566, 686, 1097
CviQI GTAC 3 cut(s) 160, 912, 1227
DdeI CTNAG 3 cut(s) 40, 130, 275
DpnI GATC 7 cut(s) 208, 419, 619, 725, 755, 927, 1021
DpnII GATC 7 cut(s) 206, 417, 617, 723, 753, 925, 1019
EaeI YGGCCR 2 cut(s) 226, 1093
Eam1104I CTCTTC 3 cut(s) 773, 1043, 1217
EarI CTCTTC 3 cut(s) 773, 1043, 1217
Ecl136II GAGCTC 2 cut(s) 145, 783
Eco147I AGGCCT 3 cut(s) 107, 328, 1170
Eco24I GRGCYC 2 cut(s) 147, 785
Eco32I GATATC 1 cut(s) 154
Eco47I GGWCC 1 cut(s) 192
Eco53kI GAGCTC 2 cut(s) 145, 783
EcoICRI GAGCTC 2 cut(s) 145, 783
EcoO109I RGGNCCY 1 cut(s) 192
EcoRII CCWGG 1 cut(s) 147
EcoRV GATATC 1 cut(s) 154
EcoT38I GRGCYC 2 cut(s) 147, 785
FaeI CATG 6 cut(s) 16, 177, 511, 569, 689, 1100
FatI CATG 6 cut(s) 12, 173, 507, 565, 685, 1096
FauI CCCGC 1 cut(s) 129
FbaI TGATCA 1 cut(s) 925
Fnu4HI GCNGC 6 cut(s) 69, 82, 91, 100, 311, 314
FokI GGATG 3 cut(s) 833, 866, 912
FriOI GRGCYC 2 cut(s) 147, 785
Fsp4HI GCNGC 6 cut(s) 69, 82, 91, 100, 311, 314
FspBI CTAG 1 cut(s) 915
GlaI GCGC 2 cut(s) 402, 966
GluI GCNGC 6 cut(s) 69, 82, 91, 100, 311, 314
GsuI CTGGAG 1 cut(s) 131
HaeIII GGCC 6 cut(s) 107, 228, 328, 1095, 1105, 1170
HapII CCGG 2 cut(s) 189, 225
HhaI GCGC 2 cut(s) 403, 967
Hin1II CATG 6 cut(s) 16, 177, 511, 569, 689, 1100
Hin6I GCGC 2 cut(s) 401, 965
HinP1I GCGC 2 cut(s) 401, 965
HindIII AAGCTT 2 cut(s) 461, 1067
HinfI GANTC 1 cut(s) 671
HpaII CCGG 2 cut(s) 189, 225
HphI GGTGA 4 cut(s) 325, 373, 850, 935
Hpy166II GTNNAC 2 cut(s) 469, 912
Hpy188I TCNGA 9 cut(s) 25, 112, 344, 422, 448, 655, 850, 883, 955
Hpy188III TCNNGA 5 cut(s) 407, 508, 615, 686, 1249
Hpy8I GTNNAC 2 cut(s) 469, 912
HpyAV CCTTC 5 cut(s) 205, 358, 424, 790, 1094
HpyCH4III ACNGT 1 cut(s) 379
HpyCH4IV ACGT 1 cut(s) 136
HpyCH4V TGCA 3 cut(s) 177, 529, 1120
HpyF3I CTNAG 3 cut(s) 40, 130, 275
HpySE526I ACGT 1 cut(s) 136
Hsp92II CATG 6 cut(s) 16, 177, 511, 569, 689, 1100
HspAI GCGC 2 cut(s) 401, 965
KroI GCCGGC 1 cut(s) 224
KroNI GCCGGC 1 cut(s) 226
Ksp22I TGATCA 1 cut(s) 925
Kzo9I GATC 7 cut(s) 206, 417, 617, 723, 753, 925, 1019
LguI GCTCTTC 1 cut(s) 773
LmnI GCTCC 2 cut(s) 150, 788
Lsp1109I GCAGC 6 cut(s) 80, 93, 102, 111, 322, 325
LweI GCATC 5 cut(s) 93, 412, 682, 976, 1133
MaeI CTAG 1 cut(s) 915
MaeII ACGT 1 cut(s) 136
MaeIII GTNAC 2 cut(s) 43, 373
MalI GATC 7 cut(s) 208, 419, 619, 725, 755, 927, 1021
MboI GATC 7 cut(s) 206, 417, 617, 723, 753, 925, 1019
MboII GAAGA 4 cut(s) 125, 790, 1060, 1234
MhlI GDGCHC 2 cut(s) 147, 785
MlsI TGGCCA 1 cut(s) 1095
MluCI AATT 4 cut(s) 520, 832, 1077, 1259
MluNI TGGCCA 1 cut(s) 1095
MlyI GAGTC 1 cut(s) 680
Mox20I TGGCCA 1 cut(s) 1095
MroNI GCCGGC 1 cut(s) 224
MscI TGGCCA 1 cut(s) 1095
MseI TTAA 5 cut(s) 459, 636, 678, 699, 1184
MslI CAYNNNNRTG 3 cut(s) 302, 690, 819
Msp20I TGGCCA 1 cut(s) 1095
MspCI CTTAAG 2 cut(s) 635, 1183
MspI CCGG 2 cut(s) 189, 225
MspR9I CCNGG 2 cut(s) 149, 190
MvaI CCWGG 1 cut(s) 149
MvnI CGCG 1 cut(s) 439
NaeI GCCGGC 1 cut(s) 226
NciI CCSGG 1 cut(s) 190
NdeII GATC 7 cut(s) 206, 417, 617, 723, 753, 925, 1019
NgoMIV GCCGGC 1 cut(s) 224
NlaIII CATG 6 cut(s) 16, 177, 511, 569, 689, 1100
NlaIV GGNNCC 3 cut(s) 193, 542, 880
NmuCI GTSAC 1 cut(s) 373
NspI RCATGY 1 cut(s) 569
PagI TCATGA 2 cut(s) 507, 685
PceI AGGCCT 3 cut(s) 107, 328, 1170
PciSI GCTCTTC 1 cut(s) 773
PcsI WCGNNNNNNNCGW 1 cut(s) 348
PdiI GCCGGC 1 cut(s) 226
PfoI TCCNGGA 1 cut(s) 147
PkrI GCNGC 6 cut(s) 70, 83, 92, 101, 312, 315
PleI GAGTC 1 cut(s) 679
PpsI GAGTC 1 cut(s) 679
PpuMI RGGWCCY 1 cut(s) 192
PshBI ATTAAT 1 cut(s) 699
Psp124BI GAGCTC 2 cut(s) 147, 785
Psp5II RGGWCCY 1 cut(s) 192
Psp6I CCWGG 1 cut(s) 147
PspGI CCWGG 1 cut(s) 147
PspN4I GGNNCC 3 cut(s) 193, 542, 880
PspPI GGNCC 2 cut(s) 192, 1103
PspPPI RGGWCCY 1 cut(s) 192
RsaI GTAC 3 cut(s) 161, 913, 1228
RsaNI GTAC 3 cut(s) 160, 912, 1227
RseI CAYNNNNRTG 3 cut(s) 302, 690, 819
SacI GAGCTC 2 cut(s) 147, 785
SapI GCTCTTC 1 cut(s) 773
SaqAI TTAA 5 cut(s) 459, 636, 678, 699, 1184
SatI GCNGC 6 cut(s) 69, 82, 91, 100, 311, 314
Sau3AI GATC 7 cut(s) 206, 417, 617, 723, 753, 925, 1019
Sau96I GGNCC 2 cut(s) 192, 1103
SchI GAGTC 1 cut(s) 680
ScrFI CCNGG 2 cut(s) 149, 190
SduI GDGCHC 2 cut(s) 147, 785
SfaNI GCATC 5 cut(s) 93, 412, 682, 976, 1133
SinI GGWCC 1 cut(s) 192
SmiMI CAYNNNNRTG 3 cut(s) 302, 690, 819
SmlI CTYRAG 3 cut(s) 635, 1183, 1249
SmoI CTYRAG 3 cut(s) 635, 1183, 1249
SpeI ACTAGT 1 cut(s) 914
Sse9I AATT 4 cut(s) 520, 832, 1077, 1259
SseBI AGGCCT 3 cut(s) 107, 328, 1170
SsiI CCGC 4 cut(s) 122, 368, 439, 876
SspMI CTAG 1 cut(s) 915
SstI GAGCTC 2 cut(s) 147, 785
StuI AGGCCT 3 cut(s) 107, 328, 1170
StyD4I CCNGG 2 cut(s) 147, 188
TaaI ACNGT 1 cut(s) 379
TaiI ACGT 1 cut(s) 139
TaqI TCGA 4 cut(s) 141, 239, 674, 986
TasI AATT 4 cut(s) 520, 832, 1077, 1259
TatI WGTACW 2 cut(s) 911, 1226
Tru1I TTAA 5 cut(s) 459, 636, 678, 699, 1184
Tru9I TTAA 5 cut(s) 459, 636, 678, 699, 1184
TscAI CASTG 4 cut(s) 216, 283, 480, 984
TseFI GTSAC 1 cut(s) 373
TseI GCWGC 6 cut(s) 68, 81, 90, 99, 310, 313
Tsp45I GTSAC 1 cut(s) 373
TspDTI ATGAA 4 cut(s) 259, 496, 524, 1113
TspRI CASTG 4 cut(s) 216, 283, 480, 984
Vha464I CTTAAG 2 cut(s) 635, 1183
VpaK11BI GGWCC 1 cut(s) 192
VspI ATTAAT 1 cut(s) 699
XapI RAATTY 1 cut(s) 1259
XceI RCATGY 1 cut(s) 569
XcmI CCANNNNNNNNNTGG 1 cut(s) 703
XspI CTAG 1 cut(s) 915
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.