pycom07g02110

Myosin heavy chain kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0014503
Physical Location & Seq
Reverse (-)
1713913 .. 1715214
1302 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g02110.1

Sequence Viewer

Length: 1302 bp
ATGTTTCATCCAATGAATTCCACTCTGTTAGAAAACAAACGTCTCTCAACAACTCCCTTGCTCTCTGCACCAGCAACAAGCACCAGCAGCAGCACCAGCAGCGAGGCCGATGACAGCCCGCCGGATTCAGGCCGCTTTTCGCTCCAAGATCATGTCAAGTATCAATTCCCCTGCGTACCCTTATCCAGGTCCTTCTCGTATCGATCGTTGGCGGTGCTCCCTGGACATGTAGGCTCGGTTTCGTGCTTGGCCTTGTGTGGTGAATTCATCCTCAGCGCCTCACAAGGCAAAGACATCATAGTCTGGCAACAGCCGGATTTAAGGCCTTTCACGAAATTCGGACAAGGCGATGGCAGCGTCAAGGCGGTTGTTGCTGTCGGGAACAAGGTGTTTACAGCGCACCAGGACAGCAGGATCAGGGTTTGGAAGGTTTCGCGGAGGTCGGAGAATGTGTTTAGGCTTGTCGACACGCTTCCAACAACGAAAGACTATTTGGGTAAGTTCATGAAGCAGAGTAATTATGTCCAAACTCGGAGACATCACAAGCGATTGTGGATCGAACACGCGGATAGTATATCGTGTTTGGCTTTCCATAACGAGTTGATTTACTCTGGTTCATGGGACAAGACTCTGAAGGTGTGGAGAATCTCGGATTTGAAGTGCGTTGAGTCGATTAAGGCTCACGACGACGCAATCAACGGGTTGGTGGCAAGTAAAGGGACTGTGTATTCTGCTTCTGCAGATGGGAAAATCAAAGCTTGGGGAAAAGAGGGGAAGAAGAAGAGCACACATTGCTTGAAGGGTATTCTGGAGGGGCACAAGGATGTTTCGCTTAACTCCGTGATCGTTTCAGAGGATGGGAGATGGGTTTATGGAGGTGGCTCCGACGGGTTTGTGATGGGATGGGAAGCGAGTAATAGCAATACTAATGGTGGTGATGTTCAAAACAAACTTGTGGTAAGTTGGAAGCTGGTTTGCGAAACAAAAGCACATGGGATGGCGGTTTTGTCGATGTGTTTGATGGGGGAGATGCTGTGCAGCGGCTCGGCGGATAAGAGCATCGGCATATGGAAGAGAGAGGCTTTCGGTAAGCTATGTAAAGTTGGGGTAATTAGCGGCCATGAAGGACCAGTGAAATGCTTGCAGGCAGCACCAAATAACGTCGGCGGTGGATTCATGTTGTACAGTGGAGGACTTGATAAGAGCTTGAGAGTTTGGTGGGTTGCAAAACCCTCTAAACCCAGAGAGGAAGATACCAATTCAGTAAAAAGTTCAGAGCAATTTCCCATTATTTTGTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

434

Amino Acids

47.38

Weight (kDa)

9.12

Isoelectric Point (pI)

38.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 73 - 306 9.9e-18 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 125 - 275 1.4e-06 WDHD1 first WD40 domain
WDR55 PF24796 175 - 386 1.7e-06 WDR55
Beta-prop_WDR3_1st PF25173 186 - 303 2.7e-10 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 187 - 303 6e-08 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 187 - 308 1.6e-07 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 191 - 304 1.3e-09 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 197 - 306 2.1e-09 WDR3 second beta-propeller domain
Beta-prop_TEP1_2nd PF25047 197 - 308 1.1e-07 TEP-1 second beta-propeller
Beta-prop_WDR5 PF25175 270 - 410 5.6e-09 WDR5 beta-propeller domain
Beta-prop_WDR3_1st PF25173 270 - 408 5.6e-07 WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd PF25047 293 - 414 5.1e-08 TEP-1 second beta-propeller
Beta-prop_THOC3 PF25174 340 - 413 9.5e-07 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014503)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51930
fragaria_vesca FvH4_7g02030
malus_domestica MD02G1296500.v1.1 MD07G1027800.v1.1
prunus_persica Prupe.2G027900_v2.0.a1 Prupe.2G027900_v2.0.a1
pyrus_communis pycom02g24950 pycom07g02110
rosa_chinensis RchiOBHm_Chr1g0319521
rosa_laevigata RLG00000030475
rosa_multiflora Rmu_sc0002539.1_g000012
rosa_roxburghii Rroxscaffold_4G00328310
rosa_samantha Rh1AG040100 Rh1BG037000 Rh1CG041100 Rh1DG028800
rosa_wichuraiana Rw1G003240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 299
AccI GTMKAC 1 cut(s) 465
AccII CGCG 2 cut(s) 436, 566
AclWI GGATC 2 cut(s) 422, 563
AcoI YGGCCR 1 cut(s) 1117
AcsI RAATTY 3 cut(s) 16, 263, 335
AcuI CTGAAG 1 cut(s) 653
AfaI GTAC 2 cut(s) 177, 1184
AfiI CCNNNNNNNGG 2 cut(s) 128, 186
AflIII ACRYGT 1 cut(s) 226
AgsI TTSAA 3 cut(s) 658, 799, 944
AjnI CCWGG 3 cut(s) 185, 220, 402
AjuI GAANNNNNNNTTGG 2 cut(s) 476, 508
AluBI AGCT 4 cut(s) 758, 970, 1093, 1206
AluI AGCT 4 cut(s) 758, 970, 1093, 1206
Alw21I GWGCWC 2 cut(s) 219, 788
Alw26I GTCTC 2 cut(s) 47, 529
AlwI GGATC 2 cut(s) 422, 563
AoxI GGCC 5 cut(s) 105, 130, 249, 323, 1117
ApeKI GCWGC 6 cut(s) 87, 90, 99, 354, 1038, 1148
ApoI RAATTY 3 cut(s) 16, 263, 335
AspLEI GCGC 2 cut(s) 278, 400
AspS9I GGNCC 2 cut(s) 189, 1127
AsuHPI GGTGA 2 cut(s) 272, 947
AvaII GGWCC 2 cut(s) 189, 1127
BaeGI GKGCMC 1 cut(s) 819
Bbv12I GWGCWC 2 cut(s) 219, 788
BbvCI CCTCAGC 1 cut(s) 272
BbvI GCAGC 6 cut(s) 99, 102, 111, 366, 1050, 1160
BccI CCATC 8 cut(s) 344, 737, 851, 858, 892, 897, 991, 1015
BciT130I CCWGG 3 cut(s) 187, 222, 404
BcoDI GTCTC 2 cut(s) 47, 529
BfaI CTAG 1 cut(s) 1300
BfmI CTRYAG 1 cut(s) 738
BfoI RGCGCY 1 cut(s) 279
BisI GCNGC 9 cut(s) 88, 91, 100, 133, 355, 1039, 1042, 1117, 1149
BlsI GCNGC 9 cut(s) 89, 92, 101, 134, 356, 1040, 1043, 1118, 1150
Bme1390I CCNGG 3 cut(s) 187, 222, 404
Bme18I GGWCC 2 cut(s) 189, 1127
BmgT120I GGNCC 2 cut(s) 189, 1127
BmiI GGNNCC 1 cut(s) 883
BmrFI CCNGG 3 cut(s) 187, 222, 404
BmsI GCATC 2 cut(s) 1020, 1068
BpmI CTGGAG 1 cut(s) 830
Bpu10I CCTNAGC 1 cut(s) 272
BpuEI CTTGAG 1 cut(s) 1228
Bsa29I ATCGAT 1 cut(s) 202
BsaJI CCNNGG 1 cut(s) 220
BsaXI ACNNNNNCTCC 4 cut(s) 437, 467, 1019, 1049
Bsc4I CCNNNNNNNGG 2 cut(s) 128, 186
Bse1I ACTGG 1 cut(s) 1130
Bse3DI GCAATG 1 cut(s) 790
BseBI CCWGG 3 cut(s) 187, 222, 404
BseCI ATCGAT 1 cut(s) 202
BseDI CCNNGG 1 cut(s) 220
BseGI GGATG 6 cut(s) 7, 267, 829, 862, 908, 1002
BseLI CCNNNNNNNGG 2 cut(s) 128, 186
BseMI GCAATG 1 cut(s) 790
BseMII CTCAG 1 cut(s) 286
BseNI ACTGG 1 cut(s) 1130
BseSI GKGCMC 1 cut(s) 819
BseXI GCAGC 6 cut(s) 99, 102, 111, 366, 1050, 1160
BsgI GTGCAG 2 cut(s) 51, 1057
Bsh1236I CGCG 2 cut(s) 436, 566
Bsh1285I CGRYCG 1 cut(s) 206
BshFI GGCC 5 cut(s) 107, 132, 251, 325, 1119
BshVI ATCGAT 1 cut(s) 202
BsiEI CGRYCG 1 cut(s) 206
BsiHKAI GWGCWC 2 cut(s) 219, 788
BsiSI CCGG 2 cut(s) 122, 314
BslFI GGGAC 2 cut(s) 635, 733
BslI CCNNNNNNNGG 2 cut(s) 128, 186
BsmAI GTCTC 2 cut(s) 47, 529
BsmBI CGTCTC 1 cut(s) 47
BsmFI GGGAC 2 cut(s) 635, 733
BsnI GGCC 5 cut(s) 107, 132, 251, 325, 1119
Bsp1286I GDGCHC 3 cut(s) 219, 788, 819
Bsp1407I TGTACA 1 cut(s) 1182
Bsp143I GATC 5 cut(s) 148, 203, 414, 555, 843
BspANI GGCC 5 cut(s) 107, 132, 251, 325, 1119
BspCNI CTCAG 1 cut(s) 285
BspDI ATCGAT 1 cut(s) 202
BspFNI CGCG 2 cut(s) 436, 566
BspHI TCATGA 1 cut(s) 504
BspLI GGNNCC 1 cut(s) 883
BspMAI CTGCAG 1 cut(s) 742
BspPI GGATC 2 cut(s) 422, 563
BspQI GCTCTTC 1 cut(s) 776
BsrDI GCAATG 1 cut(s) 790
BsrGI TGTACA 1 cut(s) 1182
BsrI ACTGG 1 cut(s) 1130
BssECI CCNNGG 1 cut(s) 220
BssMI GATC 5 cut(s) 148, 203, 414, 555, 843
Bst2UI CCWGG 3 cut(s) 187, 222, 404
Bst4CI ACNGT 2 cut(s) 724, 1187
Bst6I CTCTTC 2 cut(s) 776, 1067
BstAPI GCANNNNNTGC 1 cut(s) 792
BstAUI TGTACA 1 cut(s) 1182
BstC8I GCNNGC 3 cut(s) 119, 1142, 1146
BstDEI CTNAG 1 cut(s) 272
BstENI CCTNNNNNAGG 1 cut(s) 184
BstF5I GGATG 6 cut(s) 7, 267, 829, 862, 908, 1002
BstFNI CGCG 2 cut(s) 436, 566
BstH2I RGCGCY 1 cut(s) 279
BstHHI GCGC 2 cut(s) 278, 400
BstKTI GATC 5 cut(s) 151, 206, 417, 558, 846
BstMAI GTCTC 2 cut(s) 47, 529
BstMBI GATC 5 cut(s) 148, 203, 414, 555, 843
BstMCI CGRYCG 1 cut(s) 206
BstMWI GCNNNNNNNGC 6 cut(s) 87, 96, 99, 354, 371, 792
BstNI CCWGG 3 cut(s) 187, 222, 404
BstNSI RCATGY 1 cut(s) 230
BstSCI CCNGG 3 cut(s) 185, 220, 402
BstSFI CTRYAG 1 cut(s) 738
BstSLI GKGCMC 1 cut(s) 819
BstUI CGCG 2 cut(s) 436, 566
BstV1I GCAGC 6 cut(s) 99, 102, 111, 366, 1050, 1160
Bsu15I ATCGAT 1 cut(s) 202
BsuRI GGCC 5 cut(s) 107, 132, 251, 325, 1119
BsuTUI ATCGAT 1 cut(s) 202
BtgZI GCGATG 1 cut(s) 363
BtsCI GGATG 6 cut(s) 7, 267, 829, 862, 908, 1002
BtsIMutI CAGTG 2 cut(s) 1137, 1192
Cac8I GCNNGC 3 cut(s) 119, 1142, 1146
CciI TCATGA 1 cut(s) 504
CfoI GCGC 2 cut(s) 278, 400
Cfr13I GGNCC 2 cut(s) 189, 1127
ClaI ATCGAT 1 cut(s) 202
CseI GACGC 2 cut(s) 346, 698
Csp6I GTAC 2 cut(s) 176, 1183
CviAII CATG 7 cut(s) 152, 227, 505, 618, 992, 1121, 1177
CviQI GTAC 2 cut(s) 176, 1183
DdeI CTNAG 1 cut(s) 272
DpnI GATC 5 cut(s) 150, 205, 416, 557, 845
DpnII GATC 5 cut(s) 148, 203, 414, 555, 843
DrdI GACNNNNNNGTC 1 cut(s) 299
DseDI GACNNNNNNGTC 1 cut(s) 299
EaeI YGGCCR 1 cut(s) 1117
Eam1104I CTCTTC 2 cut(s) 776, 1067
EarI CTCTTC 2 cut(s) 776, 1067
EciI GGCGGA 1 cut(s) 1064
Eco147I AGGCCT 1 cut(s) 325
Eco47I GGWCC 2 cut(s) 189, 1127
Eco57I CTGAAG 1 cut(s) 653
EcoNI CCTNNNNNAGG 1 cut(s) 184
EcoO109I RGGNCCY 1 cut(s) 189
EcoRI GAATTC 2 cut(s) 16, 263
EcoRII CCWGG 3 cut(s) 185, 220, 402
Esp3I CGTCTC 1 cut(s) 47
FaeI CATG 7 cut(s) 155, 230, 508, 621, 995, 1124, 1180
FaqI GGGAC 2 cut(s) 635, 733
FatI CATG 7 cut(s) 151, 226, 504, 617, 991, 1120, 1176
FauI CCCGC 1 cut(s) 126
FauNDI CATATG 1 cut(s) 1067
FblI GTMKAC 1 cut(s) 465
Fnu4HI GCNGC 9 cut(s) 88, 91, 100, 133, 355, 1039, 1042, 1117, 1149
FokI GGATG 5 cut(s) 254, 836, 869, 915, 1009
Fsp4HI GCNGC 9 cut(s) 88, 91, 100, 133, 355, 1039, 1042, 1117, 1149
FspBI CTAG 1 cut(s) 1300
GlaI GCGC 2 cut(s) 277, 399
GluI GCNGC 9 cut(s) 88, 91, 100, 133, 355, 1039, 1042, 1117, 1149
GsuI CTGGAG 1 cut(s) 830
HaeII RGCGCY 1 cut(s) 279
HaeIII GGCC 5 cut(s) 107, 132, 251, 325, 1119
HapII CCGG 2 cut(s) 122, 314
HgaI GACGC 2 cut(s) 346, 698
HhaI GCGC 2 cut(s) 278, 400
Hin1II CATG 7 cut(s) 155, 230, 508, 621, 995, 1124, 1180
Hin6I GCGC 2 cut(s) 276, 398
HinP1I GCGC 2 cut(s) 276, 398
HincII GTYRAC 1 cut(s) 466
HindII GTYRAC 1 cut(s) 466
HindIII AAGCTT 1 cut(s) 756
HinfI GANTC 5 cut(s) 125, 628, 645, 668, 1173
HpaII CCGG 2 cut(s) 122, 314
HphI GGTGA 2 cut(s) 272, 947
Hpy166II GTNNAC 2 cut(s) 393, 466
Hpy188I TCNGA 8 cut(s) 341, 445, 534, 633, 652, 853, 886, 1276
Hpy188III TCNNGA 5 cut(s) 331, 379, 505, 683, 809
Hpy8I GTNNAC 2 cut(s) 393, 466
Hpy99I CGWCG 4 cut(s) 689, 692, 890, 1166
HpyAV CCTTC 5 cut(s) 202, 421, 628, 793, 1118
HpyCH4III ACNGT 2 cut(s) 724, 1187
HpyCH4IV ACGT 2 cut(s) 40, 1161
HpyCH4V TGCA 5 cut(s) 68, 740, 1038, 1144, 1226
HpyF10VI GCNNNNNNNGC 6 cut(s) 87, 96, 99, 354, 371, 792
HpyF3I CTNAG 1 cut(s) 272
HpySE526I ACGT 2 cut(s) 40, 1161
Hsp92II CATG 7 cut(s) 155, 230, 508, 621, 995, 1124, 1180
HspAI GCGC 2 cut(s) 276, 398
Kzo9I GATC 5 cut(s) 148, 203, 414, 555, 843
LguI GCTCTTC 1 cut(s) 776
LmnI GCTCC 3 cut(s) 147, 222, 887
Lsp1109I GCAGC 6 cut(s) 99, 102, 111, 366, 1050, 1160
LweI GCATC 2 cut(s) 1020, 1068
MaeI CTAG 1 cut(s) 1300
MaeII ACGT 2 cut(s) 40, 1161
MalI GATC 5 cut(s) 150, 205, 416, 557, 845
MboI GATC 5 cut(s) 148, 203, 414, 555, 843
MboII GAAGA 5 cut(s) 787, 790, 793, 1084, 1262
MhlI GDGCHC 3 cut(s) 219, 788, 819
MluCI AATT 8 cut(s) 16, 164, 263, 335, 517, 1110, 1258, 1280
MlyI GAGTC 2 cut(s) 622, 677
MmeI TCCRAC 4 cut(s) 423, 500, 909, 944
MseI TTAA 3 cut(s) 320, 675, 834
MslI CAYNNNNRTG 1 cut(s) 822
MspA1I CMGCKG 1 cut(s) 1041
MspI CCGG 2 cut(s) 122, 314
MspR9I CCNGG 3 cut(s) 187, 222, 404
MvaI CCWGG 3 cut(s) 187, 222, 404
MvnI CGCG 2 cut(s) 436, 566
MwoI GCNNNNNNNGC 6 cut(s) 87, 96, 99, 354, 371, 792
NdeI CATATG 1 cut(s) 1067
NdeII GATC 5 cut(s) 148, 203, 414, 555, 843
NlaIII CATG 7 cut(s) 155, 230, 508, 621, 995, 1124, 1180
NlaIV GGNNCC 1 cut(s) 883
NmeAIII GCCGAG 1 cut(s) 1025
NspI RCATGY 1 cut(s) 230
PagI TCATGA 1 cut(s) 504
PceI AGGCCT 1 cut(s) 325
PciI ACATGT 1 cut(s) 226
PciSI GCTCTTC 1 cut(s) 776
PcsI WCGNNNNNNNCGW 2 cut(s) 203, 345
PfeI GAWTC 3 cut(s) 125, 645, 1173
PkrI GCNGC 9 cut(s) 89, 92, 101, 134, 356, 1040, 1043, 1118, 1150
Ple19I CGATCG 1 cut(s) 206
PleI GAGTC 2 cut(s) 622, 676
PpsI GAGTC 2 cut(s) 622, 676
PpuMI RGGWCCY 1 cut(s) 189
PscI ACATGT 1 cut(s) 226
Psp5II RGGWCCY 1 cut(s) 189
Psp6I CCWGG 3 cut(s) 185, 220, 402
PspGI CCWGG 3 cut(s) 185, 220, 402
PspN4I GGNNCC 1 cut(s) 883
PspPI GGNCC 2 cut(s) 189, 1127
PspPPI RGGWCCY 1 cut(s) 189
PstI CTGCAG 1 cut(s) 742
PvuI CGATCG 1 cut(s) 206
RsaI GTAC 2 cut(s) 177, 1184
RsaNI GTAC 2 cut(s) 176, 1183
RseI CAYNNNNRTG 1 cut(s) 822
SalI GTCGAC 1 cut(s) 464
SapI GCTCTTC 1 cut(s) 776
SaqAI TTAA 3 cut(s) 320, 675, 834
SatI GCNGC 9 cut(s) 88, 91, 100, 133, 355, 1039, 1042, 1117, 1149
Sau3AI GATC 5 cut(s) 148, 203, 414, 555, 843
Sau96I GGNCC 2 cut(s) 189, 1127
SchI GAGTC 2 cut(s) 622, 677
ScrFI CCNGG 3 cut(s) 187, 222, 404
SduI GDGCHC 3 cut(s) 219, 788, 819
SfaNI GCATC 2 cut(s) 1020, 1068
SfcI CTRYAG 1 cut(s) 738
SinI GGWCC 2 cut(s) 189, 1127
SmiMI CAYNNNNRTG 1 cut(s) 822
SmlI CTYRAG 1 cut(s) 1207
SmoI CTYRAG 1 cut(s) 1207
Sse9I AATT 8 cut(s) 16, 164, 263, 335, 517, 1110, 1258, 1280
SseBI AGGCCT 1 cut(s) 325
SspMI CTAG 1 cut(s) 1300
StuI AGGCCT 1 cut(s) 325
StyD4I CCNGG 3 cut(s) 185, 220, 402
TaaI ACNGT 2 cut(s) 724, 1187
TaiI ACGT 2 cut(s) 43, 1164
TaqI TCGA 5 cut(s) 202, 465, 558, 671, 1010
TasI AATT 8 cut(s) 16, 164, 263, 335, 517, 1110, 1258, 1280
TatI WGTACW 1 cut(s) 1182
TauI GCSGC 3 cut(s) 135, 1044, 1119
TfiI GAWTC 3 cut(s) 125, 645, 1173
Tru1I TTAA 3 cut(s) 320, 675, 834
Tru9I TTAA 3 cut(s) 320, 675, 834
TscAI CASTG 2 cut(s) 1137, 1192
TseI GCWGC 6 cut(s) 87, 90, 99, 354, 1038, 1148
TspDTI ATGAA 7 cut(s) 29, 256, 493, 521, 606, 1137, 1165
TspGWI ACGGA 1 cut(s) 829
TspRI CASTG 2 cut(s) 1137, 1192
VpaK11BI GGWCC 2 cut(s) 189, 1127
XagI CCTNNNNNAGG 1 cut(s) 184
XapI RAATTY 3 cut(s) 16, 263, 335
XceI RCATGY 1 cut(s) 230
XmiI GTMKAC 1 cut(s) 465
XspI CTAG 1 cut(s) 1300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.