MD02G1296500.v1.1

Myosin heavy chain kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0014503
Physical Location & Seq
Forward (+)
35175049 .. 35176353
1305 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1296500.v1.1.491

Sequence Viewer

Length: 1305 bp
ATGTTTCATCCAATGAATTCTACTCTGTTAGAAAACAAGCGTCTCTCAACAAGTCCCTTGCTCTCTTCCTCAGCAGCAAGCACCACCAGAAGCAGTAGCACGACCCACGAGACTGATGAAAGCCCGCGGGATCCAGGCCCCTTTTCACTTCAAGATCACGTTAAGTATCAATTTCCCTGCATACCCTTATTGGGGTCCTTCTCGTATCGATCGTTGGCGGTGCTCTCCGGACACGTAGGTTCGGTTTCGTGTCTAGCCTTGTGTGGTGAATTCATCCTCAGCGCCTCACAAGGCAAAGACATCATAGTTTGGCAACAGCCGGATTTTAGTCCGTTCATGAAATTCGGACAAGGCGATGGAAGCGTCAAGGCGGTTGTTGCTGTCGGAAACAAGGTGTTTACAGCGCACCAGGACAGCAAGATCAGGGTTTGGAAGGTTTCGCGGAGGTCGGAGAATGTGTTTAGGCTTGTCGACACGCTTCCCACAACAAAAGACTATTTGGGGAAGTTCATGAAGCAAAGTAATTATGTCCAAACTCGAAGACATCACAAGCGGTTGTGGATCGAACATGCAGATAGTATATCGTGTTTGGCTTTCCATAACGAGTTGATTTACTCTGGATCATGGGACAAGACGCTCAAGGTGTGGAGGATTTCGGATTTGAAGTGCGTAGAGTCGATTAAGGCTCATGACGATGCAATCAACGGGTTGGTGGCAAGTAAAGGGATTGTGTATTCGGCTTCTGCAGATGGGAAAATCAAAGCTTGGGGAAAAGAGGGGAAGAAGAGCACACACTGTTTGAAGGGTATTTTGCAGGGGCATAATGATGTTTCGCTTAACTCCGTGATCGTTTCTGAGGATGAGAGATGGGTTTATGGAGGTGGCTCGGACGGGTTTGTGATGGGATGGGAAGCAAGTAGTTGTAGCACTTCAAATGGTGGTGATGTTCAAAACCAACTTGAGGTGAGTTGGAAGCTGGTTTGCGAAACAAAAGCGCATCGAATGGCGGTTTTGTCAATGTGTTTGATGGGGGAGATGTTGTGCAGCGGCTCGGCGGATAAGAGCATAGGCATTTGGAAGATGGAGGCTTTTGGTAAGCTTTGTAAATTTGGGGTGATTAGCGGCCATGAAGGGCCAGTGAAGTGCTTGCAGGCAGCAACATATAATGTCGGCGGTGGATTCATGTTGTATAGTGGAGGACTTGATAAGAGCTTGAGAGTTTGGTGGGTTGCAAAACCCTCTAAAACCAGAGAGGAAGATACCAATTCAGCAAAAAGTTCAGAGGAATTTCCCATTGTTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

435

Amino Acids

47.67

Weight (kDa)

8.7

Isoelectric Point (pI)

38.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR5 PF25175 75 - 273 5.5e-17 WDR5 beta-propeller domain
Beta-prop_SCAP PF24017 79 - 257 8.2e-06 SCAP Beta-propeller
WD40_WDHD1_1st PF24817 127 - 362 2.2e-08 WDHD1 first WD40 domain
WDR55 PF24796 177 - 389 2.3e-07 WDR55
Beta-prop_THOC3 PF25174 187 - 383 9.3e-14 THOC3 beta-propeller domain
Beta-prop_WDR3_1st PF25173 188 - 304 3.5e-11 WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd PF25047 189 - 303 4e-07 TEP-1 second beta-propeller
WD40_CDC20-Fz PF24807 189 - 309 4e-07 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 193 - 420 2.4e-15 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 198 - 306 1.3e-08 WDR3 second beta-propeller domain
Beta-prop_WDR5 PF25175 244 - 412 3.6e-12 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 321 - 414 1.2e-07 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 325 - 411 1.3e-06 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 338 - 423 7.1e-07 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014503)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51930
fragaria_vesca FvH4_7g02030
malus_domestica MD02G1296500.v1.1 MD07G1027800.v1.1
prunus_persica Prupe.2G027900_v2.0.a1 Prupe.2G027900_v2.0.a1
pyrus_communis pycom02g24950 pycom07g02110
rosa_chinensis RchiOBHm_Chr1g0319521
rosa_laevigata RLG00000030475
rosa_multiflora Rmu_sc0002539.1_g000012
rosa_roxburghii Rroxscaffold_4G00328310
rosa_samantha Rh1AG040100 Rh1BG037000 Rh1CG041100 Rh1DG028800
rosa_wichuraiana Rw1G003240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 471
AccII CGCG 2 cut(s) 127, 442
AccIII TCCGGA 1 cut(s) 227
AclWI GGATC 4 cut(s) 125, 138, 569, 628
AcoI YGGCCR 1 cut(s) 1123
AcsI RAATTY 5 cut(s) 16, 269, 341, 1106, 1286
AfiI CCNNNNNNNGG 3 cut(s) 191, 192, 961
AflIII ACRYGT 1 cut(s) 232
AgsI TTSAA 5 cut(s) 152, 664, 802, 933, 950
AjnI CCWGG 2 cut(s) 133, 408
AluBI AGCT 4 cut(s) 764, 976, 1099, 1212
AluI AGCT 4 cut(s) 764, 976, 1099, 1212
Alw21I GWGCWC 2 cut(s) 225, 791
Alw26I GTCTC 2 cut(s) 47, 104
AlwI GGATC 4 cut(s) 125, 138, 569, 628
Aor13HI TCCGGA 1 cut(s) 227
AoxI GGCC 3 cut(s) 136, 1123, 1133
ApeKI GCWGC 3 cut(s) 74, 1044, 1154
ApoI RAATTY 5 cut(s) 16, 269, 341, 1106, 1286
AspLEI GCGC 3 cut(s) 284, 406, 997
AspS9I GGNCC 3 cut(s) 137, 195, 1133
AsuHPI GGTGA 4 cut(s) 278, 953, 976, 1126
AvaII GGWCC 1 cut(s) 195
BamHI GGATCC 1 cut(s) 130
BauI CACGAG 1 cut(s) 107
BbsI GAAGAC 1 cut(s) 547
Bbv12I GWGCWC 2 cut(s) 225, 791
BbvCI CCTCAGC 2 cut(s) 70, 278
BbvI GCAGC 3 cut(s) 86, 1056, 1166
BccI CCATC 7 cut(s) 350, 743, 861, 895, 900, 1021, 1075
BciT130I CCWGG 2 cut(s) 135, 410
BcoDI GTCTC 2 cut(s) 47, 104
BfaI CTAG 1 cut(s) 254
BfmI CTRYAG 1 cut(s) 744
BfoI RGCGCY 1 cut(s) 285
BisI GCNGC 5 cut(s) 75, 1045, 1048, 1123, 1155
BlsI GCNGC 5 cut(s) 76, 1046, 1049, 1124, 1156
Bme1390I CCNGG 2 cut(s) 135, 410
Bme18I GGWCC 1 cut(s) 195
BmgT120I GGNCC 3 cut(s) 137, 195, 1133
BmiI GGNNCC 3 cut(s) 132, 139, 196
BmrFI CCNGG 2 cut(s) 135, 410
BmsI GCATC 2 cut(s) 685, 1006
BpiI GAAGAC 1 cut(s) 547
Bpu10I CCTNAGC 2 cut(s) 70, 278
BpuEI CTTGAG 3 cut(s) 623, 980, 1234
Bsa29I ATCGAT 1 cut(s) 208
BsaAI YACGTR 1 cut(s) 235
BsaJI CCNNGG 1 cut(s) 125
BsaWI WCCGGW 1 cut(s) 227
BsaXI ACNNNNNCTCC 4 cut(s) 443, 473, 1025, 1055
Bsc4I CCNNNNNNNGG 3 cut(s) 191, 192, 961
Bse1I ACTGG 1 cut(s) 1136
BseAI TCCGGA 1 cut(s) 227
BseBI CCWGG 2 cut(s) 135, 410
BseCI ATCGAT 1 cut(s) 208
BseDI CCNNGG 1 cut(s) 125
BseGI GGATG 4 cut(s) 7, 273, 865, 911
BseLI CCNNNNNNNGG 3 cut(s) 191, 192, 961
BseMII CTCAG 3 cut(s) 84, 292, 846
BseNI ACTGG 1 cut(s) 1136
BseXI GCAGC 3 cut(s) 86, 1056, 1166
BsgI GTGCAG 1 cut(s) 1063
Bsh1236I CGCG 2 cut(s) 127, 442
Bsh1285I CGRYCG 1 cut(s) 212
BshFI GGCC 3 cut(s) 138, 1125, 1135
BshVI ATCGAT 1 cut(s) 208
BsiEI CGRYCG 1 cut(s) 212
BsiHKAI GWGCWC 2 cut(s) 225, 791
BsiSI CCGG 2 cut(s) 228, 320
BslFI GGGAC 2 cut(s) 39, 641
BslI CCNNNNNNNGG 3 cut(s) 191, 192, 961
BsmAI GTCTC 2 cut(s) 47, 104
BsmBI CGTCTC 1 cut(s) 47
BsmFI GGGAC 2 cut(s) 39, 641
BsnI GGCC 3 cut(s) 138, 1125, 1135
Bsp1286I GDGCHC 2 cut(s) 225, 791
Bsp13I TCCGGA 1 cut(s) 227
Bsp143I GATC 7 cut(s) 130, 154, 209, 420, 561, 620, 846
BspANI GGCC 3 cut(s) 138, 1125, 1135
BspCNI CTCAG 3 cut(s) 83, 291, 847
BspDI ATCGAT 1 cut(s) 208
BspEI TCCGGA 1 cut(s) 227
BspFNI CGCG 2 cut(s) 127, 442
BspHI TCATGA 3 cut(s) 336, 510, 688
BspLI GGNNCC 3 cut(s) 132, 139, 196
BspMAI CTGCAG 1 cut(s) 748
BspPI GGATC 4 cut(s) 125, 138, 569, 628
BspQI GCTCTTC 1 cut(s) 779
BsrI ACTGG 1 cut(s) 1136
BssECI CCNNGG 1 cut(s) 125
BssMI GATC 7 cut(s) 130, 154, 209, 420, 561, 620, 846
BssSI CACGAG 1 cut(s) 107
Bst2BI CACGAG 1 cut(s) 107
Bst2UI CCWGG 2 cut(s) 135, 410
Bst4CI ACNGT 1 cut(s) 797
Bst6I CTCTTC 2 cut(s) 70, 779
BstBAI YACGTR 1 cut(s) 235
BstC8I GCNNGC 4 cut(s) 79, 125, 1148, 1152
BstDEI CTNAG 3 cut(s) 70, 278, 855
BstDSI CCRYGG 1 cut(s) 125
BstF5I GGATG 4 cut(s) 7, 273, 865, 911
BstFNI CGCG 2 cut(s) 127, 442
BstH2I RGCGCY 1 cut(s) 285
BstHHI GCGC 3 cut(s) 284, 406, 997
BstKTI GATC 7 cut(s) 133, 157, 212, 423, 564, 623, 849
BstMAI GTCTC 2 cut(s) 47, 104
BstMBI GATC 7 cut(s) 130, 154, 209, 420, 561, 620, 846
BstMCI CGRYCG 1 cut(s) 212
BstMWI GCNNNNNNNGC 2 cut(s) 360, 377
BstNI CCWGG 2 cut(s) 135, 410
BstNSI RCATGY 1 cut(s) 572
BstSCI CCNGG 2 cut(s) 133, 408
BstSFI CTRYAG 1 cut(s) 744
BstUI CGCG 2 cut(s) 127, 442
BstV1I GCAGC 3 cut(s) 86, 1056, 1166
BstV2I GAAGAC 1 cut(s) 547
BstX2I RGATCY 1 cut(s) 130
BstYI RGATCY 1 cut(s) 130
Bsu15I ATCGAT 1 cut(s) 208
BsuRI GGCC 3 cut(s) 138, 1125, 1135
BsuTUI ATCGAT 1 cut(s) 208
BtgI CCRYGG 1 cut(s) 125
BtgZI GCGATG 1 cut(s) 369
BtsCI GGATG 4 cut(s) 7, 273, 865, 911
BtsIMutI CAGTG 2 cut(s) 793, 1143
Cac8I GCNNGC 4 cut(s) 79, 125, 1148, 1152
CciI TCATGA 3 cut(s) 336, 510, 688
CfoI GCGC 3 cut(s) 284, 406, 997
Cfr13I GGNCC 3 cut(s) 137, 195, 1133
Cfr42I CCGCGG 1 cut(s) 128
ClaI ATCGAT 1 cut(s) 208
CseI GACGC 3 cut(s) 29, 352, 643
CviAII CATG 7 cut(s) 337, 511, 569, 624, 689, 1127, 1183
DdeI CTNAG 3 cut(s) 70, 278, 855
DpnI GATC 7 cut(s) 132, 156, 211, 422, 563, 622, 848
DpnII GATC 7 cut(s) 130, 154, 209, 420, 561, 620, 846
EaeI YGGCCR 1 cut(s) 1123
Eam1104I CTCTTC 2 cut(s) 70, 779
EarI CTCTTC 2 cut(s) 70, 779
EciI GGCGGA 1 cut(s) 1070
Eco47I GGWCC 1 cut(s) 195
EcoO109I RGGNCCY 2 cut(s) 137, 195
EcoRI GAATTC 2 cut(s) 16, 269
EcoRII CCWGG 2 cut(s) 133, 408
Esp3I CGTCTC 1 cut(s) 47
FaeI CATG 7 cut(s) 340, 514, 572, 627, 692, 1130, 1186
FaqI GGGAC 2 cut(s) 39, 641
FatI CATG 7 cut(s) 336, 510, 568, 623, 688, 1126, 1182
FauI CCCGC 2 cut(s) 120, 132
FblI GTMKAC 1 cut(s) 471
Fnu4HI GCNGC 5 cut(s) 75, 1045, 1048, 1123, 1155
FokI GGATG 3 cut(s) 260, 872, 918
Fsp4HI GCNGC 5 cut(s) 75, 1045, 1048, 1123, 1155
FspBI CTAG 1 cut(s) 254
GlaI GCGC 3 cut(s) 283, 405, 996
GluI GCNGC 5 cut(s) 75, 1045, 1048, 1123, 1155
HaeII RGCGCY 1 cut(s) 285
HaeIII GGCC 3 cut(s) 138, 1125, 1135
HapII CCGG 2 cut(s) 228, 320
HgaI GACGC 3 cut(s) 29, 352, 643
HhaI GCGC 3 cut(s) 284, 406, 997
Hin1II CATG 7 cut(s) 340, 514, 572, 627, 692, 1130, 1186
Hin6I GCGC 3 cut(s) 282, 404, 995
HinP1I GCGC 3 cut(s) 282, 404, 995
HincII GTYRAC 1 cut(s) 472
HindII GTYRAC 1 cut(s) 472
HindIII AAGCTT 2 cut(s) 762, 1097
HinfI GANTC 2 cut(s) 674, 1179
HpaII CCGG 2 cut(s) 228, 320
HphI GGTGA 4 cut(s) 278, 953, 976, 1126
Hpy166II GTNNAC 2 cut(s) 399, 472
Hpy188I TCNGA 7 cut(s) 347, 386, 451, 658, 856, 889, 1282
Hpy188III TCNNGA 6 cut(s) 152, 228, 337, 511, 618, 689
Hpy8I GTNNAC 2 cut(s) 399, 472
HpyAV CCTTC 4 cut(s) 208, 427, 796, 1124
HpyCH4III ACNGT 1 cut(s) 797
HpyCH4IV ACGT 2 cut(s) 159, 234
HpyCH4V TGCA 8 cut(s) 180, 572, 698, 746, 814, 1044, 1150, 1232
HpyF10VI GCNNNNNNNGC 2 cut(s) 360, 377
HpyF3I CTNAG 3 cut(s) 70, 278, 855
HpySE526I ACGT 2 cut(s) 159, 234
Hsp92II CATG 7 cut(s) 340, 514, 572, 627, 692, 1130, 1186
HspAI GCGC 3 cut(s) 282, 404, 995
Kpn2I TCCGGA 1 cut(s) 227
KspI CCGCGG 1 cut(s) 128
Kzo9I GATC 7 cut(s) 130, 154, 209, 420, 561, 620, 846
LguI GCTCTTC 1 cut(s) 779
Lsp1109I GCAGC 3 cut(s) 86, 1056, 1166
LweI GCATC 2 cut(s) 685, 1006
MaeI CTAG 1 cut(s) 254
MaeII ACGT 2 cut(s) 159, 234
MalI GATC 7 cut(s) 132, 156, 211, 422, 563, 622, 848
MboI GATC 7 cut(s) 130, 154, 209, 420, 561, 620, 846
MboII GAAGA 6 cut(s) 57, 552, 793, 796, 1090, 1268
MflI RGATCY 1 cut(s) 130
MhlI GDGCHC 2 cut(s) 225, 791
MluCI AATT 8 cut(s) 16, 170, 269, 341, 523, 1106, 1264, 1286
MlyI GAGTC 1 cut(s) 683
MmeI TCCRAC 3 cut(s) 364, 429, 950
MroI TCCGGA 1 cut(s) 227
MseI TTAA 3 cut(s) 162, 681, 837
MslI CAYNNNNRTG 2 cut(s) 693, 825
MspA1I CMGCKG 2 cut(s) 127, 1047
MspI CCGG 2 cut(s) 228, 320
MspR9I CCNGG 2 cut(s) 135, 410
MvaI CCWGG 2 cut(s) 135, 410
MvnI CGCG 2 cut(s) 127, 442
MwoI GCNNNNNNNGC 2 cut(s) 360, 377
NdeII GATC 7 cut(s) 130, 154, 209, 420, 561, 620, 846
NlaIII CATG 7 cut(s) 340, 514, 572, 627, 692, 1130, 1186
NlaIV GGNNCC 3 cut(s) 132, 139, 196
NmeAIII GCCGAG 1 cut(s) 1031
NspI RCATGY 1 cut(s) 572
PagI TCATGA 3 cut(s) 336, 510, 688
PciSI GCTCTTC 1 cut(s) 779
PcsI WCGNNNNNNNCGW 2 cut(s) 209, 351
PfeI GAWTC 1 cut(s) 1179
PkrI GCNGC 5 cut(s) 76, 1046, 1049, 1124, 1156
Ple19I CGATCG 1 cut(s) 212
PleI GAGTC 1 cut(s) 682
PpsI GAGTC 1 cut(s) 682
Ppu21I YACGTR 1 cut(s) 235
PpuMI RGGWCCY 1 cut(s) 195
Psp5II RGGWCCY 1 cut(s) 195
Psp6I CCWGG 2 cut(s) 133, 408
PspGI CCWGG 2 cut(s) 133, 408
PspN4I GGNNCC 3 cut(s) 132, 139, 196
PspPI GGNCC 3 cut(s) 137, 195, 1133
PspPPI RGGWCCY 1 cut(s) 195
PstI CTGCAG 1 cut(s) 748
PsuI RGATCY 1 cut(s) 130
PvuI CGATCG 1 cut(s) 212
RseI CAYNNNNRTG 2 cut(s) 693, 825
SacII CCGCGG 1 cut(s) 128
SalI GTCGAC 1 cut(s) 470
SapI GCTCTTC 1 cut(s) 779
SaqAI TTAA 3 cut(s) 162, 681, 837
SatI GCNGC 5 cut(s) 75, 1045, 1048, 1123, 1155
Sau3AI GATC 7 cut(s) 130, 154, 209, 420, 561, 620, 846
Sau96I GGNCC 3 cut(s) 137, 195, 1133
SchI GAGTC 1 cut(s) 683
ScrFI CCNGG 2 cut(s) 135, 410
SduI GDGCHC 2 cut(s) 225, 791
SfaNI GCATC 2 cut(s) 685, 1006
SfcI CTRYAG 1 cut(s) 744
Sfr303I CCGCGG 1 cut(s) 128
SgrBI CCGCGG 1 cut(s) 128
SinI GGWCC 1 cut(s) 195
SmiMI CAYNNNNRTG 2 cut(s) 693, 825
SmlI CTYRAG 3 cut(s) 638, 959, 1213
SmoI CTYRAG 3 cut(s) 638, 959, 1213
Sse9I AATT 8 cut(s) 16, 170, 269, 341, 523, 1106, 1264, 1286
SspMI CTAG 1 cut(s) 254
StyD4I CCNGG 2 cut(s) 133, 408
TaaI ACNGT 1 cut(s) 797
TaiI ACGT 2 cut(s) 162, 237
TaqI TCGA 6 cut(s) 208, 471, 538, 564, 677, 1000
TasI AATT 8 cut(s) 16, 170, 269, 341, 523, 1106, 1264, 1286
TauI GCSGC 2 cut(s) 1050, 1125
TfiI GAWTC 1 cut(s) 1179
Tru1I TTAA 3 cut(s) 162, 681, 837
Tru9I TTAA 3 cut(s) 162, 681, 837
TscAI CASTG 2 cut(s) 800, 1143
TseI GCWGC 3 cut(s) 74, 1044, 1154
TspDTI ATGAA 9 cut(s) 29, 132, 262, 325, 353, 499, 527, 1143, 1171
TspGWI ACGGA 2 cut(s) 321, 832
TspRI CASTG 2 cut(s) 800, 1143
VpaK11BI GGWCC 1 cut(s) 195
XapI RAATTY 5 cut(s) 16, 269, 341, 1106, 1286
XceI RCATGY 1 cut(s) 572
XmiI GTMKAC 1 cut(s) 471
XspI CTAG 1 cut(s) 254
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.