RLG00000030475

Myosin heavy chain kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0014503
Physical Location & Seq
Forward (+)
62202789 .. 62204033
1245 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030475

Sequence Viewer

Length: 1245 bp
ATGACTCTTTCAGAAAACAAACGTCTCTCTGTTACTCCCTTACTCTCTGCCACAGCAGCAAGCACCAGCAGCATCAGCAGCACAAGCAGCGAGGCCTCCGAAGAAAGCCCGCCGACCTCAGCACGTTTCGAGCTCCAGGATATCAAGTACCATCATTTTCCATGCACGCACTTATCCGGGTCCTTCTCCTACCGATCACTGGCTGTGCTTGCCGGCCATAAAGGCTCGATTTCGTGCCTGTCCTTATGTGGAGAGTTCATCCTCAGTGCTTCACAAGGTAAAGACATCATTGTGTGGCAGCAGCCTGATTTGAGGCCTTTCACCAAGTTCGGACAAGGCGATGGCTCGGTGAAGGCGGTTGTGACCGTTGGGAACAAGGTCTTTACGGCGCATCAAGACAGCAGGATCAGAGTTTGGAAGGTTTCGCGGAGTTCAGAGAATGTGTTTAAGCTTGTTGACACACTGCCTACAACGAAAGACTATTTGGGAAAGTTCATGAAGCAAAGCAATTATGTGCAAACGAGGAGGCACCACAAGAAGTTGTGGATTGAACATGCTGATAGTATCTCTTGTCTGGCTTTCTATAATGGGTTGATTTACTCTGGATCGTGGGATAAGACCCTTAAGGTGTGGAGGGTTTCGGATTTGAAGTGCGTAGAATCGATTAAGGCTCATGATGATGCCATTAATGGGTTGGTGGCAAGTAAAGGGATCGTGTATTCTGCTTCTGCCGATGGAAAGATCAAGGCTTGGGGAAAAGAGGGGAAGAGCTCCCATTGCTTGAAGGGTATTTTGGAGGGTCATAAGGATGTTTCGTTCAATTCGGTGATTGTTTCCGAGGATGGGAGATGGGTTTATGGAGGCGGTTCGGATGGGTTTGTGATGGGATGGGAAGTGTGTACTAGTGGTGGTGATCAAGTTGCGAGTTGGAAGTTGGTTTGCGAAACAAAAGCGCATCAAATGGCAGTGTTGTCAATGTGTTTGATGGGGGAGATGTTGTGTAGTGGATCAGCAGATAAGAGTATTGGTATATGGAAGAGAGAGGCTTTTGGGAAGCTTTCCAAAATTGGGGTTATTAGTGGCCATGAAGGGCCAGTCAGGTGCTTGCAAGCATCGCCGAAGAGAGTTGGTGGTGGTTTTATGTTGTATAGTGGAGGCCTTGACAAGAGCTTAAGAGTTTGGTGGGTTTCCAAGCACTCCACAACACAGAAGAGTACAGAGGAAAAATCCATCATCTTGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

415

Amino Acids

45.18

Weight (kDa)

9.15

Isoelectric Point (pI)

39.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_SCAP PF24017 64 - 251 2.9e-07 SCAP Beta-propeller
Beta-prop_WDR5 PF25175 70 - 272 5.4e-18 WDR5 beta-propeller domain
WDR55 PF24796 171 - 370 6.2e-08 WDR55
Beta-prop_WDR3_1st PF25173 183 - 299 3.6e-11 WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd PF25047 183 - 256 2.9e-06 TEP-1 second beta-propeller
WD40_CDC20-Fz PF24807 184 - 302 1.3e-06 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 185 - 299 4.8e-09 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 196 - 298 2.1e-08 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 197 - 302 8e-08 THOC3 beta-propeller domain
Beta-prop_EML PF23409 247 - 372 7.1e-07 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR5 PF25175 266 - 402 2.8e-10 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 286 - 399 1.6e-10 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 309 - 412 3.9e-07 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 311 - 410 1.2e-06 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 324 - 410 3.3e-08 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0014503)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51930
fragaria_vesca FvH4_7g02030
malus_domestica MD02G1296500.v1.1 MD07G1027800.v1.1
prunus_persica Prupe.2G027900_v2.0.a1 Prupe.2G027900_v2.0.a1
pyrus_communis pycom02g24950 pycom07g02110
rosa_chinensis RchiOBHm_Chr1g0319521
rosa_laevigata RLG00000030475
rosa_multiflora Rmu_sc0002539.1_g000012
rosa_roxburghii Rroxscaffold_4G00328310
rosa_samantha Rh1AG040100 Rh1BG037000 Rh1CG041100 Rh1DG028800
rosa_wichuraiana Rw1G003240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 528
AccII CGCG 1 cut(s) 427
AciI CCGC 4 cut(s) 110, 356, 427, 864
AclWI GGATC 4 cut(s) 413, 613, 719, 1015
AcoI YGGCCR 2 cut(s) 214, 1081
AfaI GTAC 3 cut(s) 149, 901, 1216
AfiI CCNNNNNNNGG 4 cut(s) 199, 690, 843, 1068
AflII CTTAAG 2 cut(s) 623, 1171
AgsI TTSAA 4 cut(s) 551, 649, 784, 820
AhlI ACTAGT 1 cut(s) 902
AjnI CCWGG 1 cut(s) 135
AjuI GAANNNNNNNTTGG 4 cut(s) 467, 499, 776, 808
AloI GAACNNNNNNTCC 2 cut(s) 800, 832
AluBI AGCT 6 cut(s) 133, 451, 771, 1057, 1170, 1242
AluI AGCT 6 cut(s) 133, 451, 771, 1057, 1170, 1242
Alw21I GWGCWC 2 cut(s) 135, 773
Alw26I GTCTC 1 cut(s) 29
AlwI GGATC 4 cut(s) 413, 613, 719, 1015
AoxI GGCC 6 cut(s) 93, 214, 314, 1081, 1091, 1156
ApeKI GCWGC 6 cut(s) 56, 69, 78, 87, 298, 301
AseI ATTAAT 1 cut(s) 687
AspLEI GCGC 2 cut(s) 391, 955
AspS9I GGNCC 2 cut(s) 180, 1091
AsuC2I CCSGG 1 cut(s) 178
AsuHPI GGTGA 4 cut(s) 313, 361, 838, 923
AvaII GGWCC 1 cut(s) 180
BalI TGGCCA 1 cut(s) 1083
BanI GGYRCC 1 cut(s) 528
BanII GRGCYC 2 cut(s) 135, 773
Bbv12I GWGCWC 2 cut(s) 135, 773
BbvCI CCTCAGC 1 cut(s) 118
BbvI GCAGC 6 cut(s) 68, 81, 90, 99, 310, 313
BceAI ACGGC 1 cut(s) 402
BciT130I CCWGG 1 cut(s) 137
BclI TGATCA 1 cut(s) 913
BcnI CCSGG 1 cut(s) 178
BcoDI GTCTC 1 cut(s) 29
BcuI ACTAGT 1 cut(s) 902
BfaI CTAG 2 cut(s) 903, 1243
BfrI CTTAAG 2 cut(s) 623, 1171
BglI GCCNNNNNGGC 1 cut(s) 222
BisI GCNGC 6 cut(s) 57, 70, 79, 88, 299, 302
BlsI GCNGC 6 cut(s) 58, 71, 80, 89, 300, 303
Bme1390I CCNGG 2 cut(s) 137, 178
Bme18I GGWCC 1 cut(s) 180
BmgT120I GGNCC 2 cut(s) 180, 1091
BmiI GGNNCC 2 cut(s) 181, 530
BmrFI CCNGG 2 cut(s) 137, 178
BmsI GCATC 5 cut(s) 81, 400, 670, 964, 1121
BpmI CTGGAG 1 cut(s) 119
Bpu10I CCTNAGC 1 cut(s) 118
BpuMI CCSGG 1 cut(s) 178
Bsa29I ATCGAT 1 cut(s) 662
BsaJI CCNNGG 1 cut(s) 837
BsaXI ACNNNNNCTCC 2 cut(s) 983, 1013
Bsc4I CCNNNNNNNGG 4 cut(s) 199, 690, 843, 1068
Bse118I RCCGGY 1 cut(s) 212
Bse1I ACTGG 2 cut(s) 204, 1094
Bse3DI GCAATG 1 cut(s) 775
BseBI CCWGG 1 cut(s) 137
BseCI ATCGAT 1 cut(s) 662
BseDI CCNNGG 1 cut(s) 837
BseGI GGATG 5 cut(s) 258, 814, 847, 877, 893
BseLI CCNNNNNNNGG 4 cut(s) 199, 690, 843, 1068
BseMI GCAATG 1 cut(s) 775
BseMII CTCAG 2 cut(s) 132, 277
BseNI ACTGG 2 cut(s) 204, 1094
BseRI GAGGAG 1 cut(s) 538
BseXI GCAGC 6 cut(s) 68, 81, 90, 99, 310, 313
Bsh1236I CGCG 1 cut(s) 427
BshFI GGCC 6 cut(s) 95, 216, 316, 1083, 1093, 1158
BshNI GGYRCC 1 cut(s) 528
BshVI ATCGAT 1 cut(s) 662
BsiHKAI GWGCWC 2 cut(s) 135, 773
BsiSI CCGG 2 cut(s) 177, 213
BslI CCNNNNNNNGG 4 cut(s) 199, 690, 843, 1068
BsmAI GTCTC 1 cut(s) 29
BsmBI CGTCTC 1 cut(s) 29
BsnI GGCC 6 cut(s) 95, 216, 316, 1083, 1093, 1158
Bsp1286I GDGCHC 2 cut(s) 135, 773
Bsp143I GATC 7 cut(s) 194, 405, 605, 711, 741, 913, 1007
BspACI CCGC 4 cut(s) 110, 356, 427, 864
BspANI GGCC 6 cut(s) 95, 216, 316, 1083, 1093, 1158
BspCNI CTCAG 2 cut(s) 131, 276
BspDI ATCGAT 1 cut(s) 662
BspFNI CGCG 1 cut(s) 427
BspHI TCATGA 2 cut(s) 495, 673
BspLI GGNNCC 2 cut(s) 181, 530
BspPI GGATC 4 cut(s) 413, 613, 719, 1015
BspQI GCTCTTC 1 cut(s) 761
BspT107I GGYRCC 1 cut(s) 528
BspTI CTTAAG 2 cut(s) 623, 1171
BsrDI GCAATG 1 cut(s) 775
BsrFI RCCGGY 1 cut(s) 212
BsrI ACTGG 2 cut(s) 204, 1094
BssAI RCCGGY 1 cut(s) 212
BssECI CCNNGG 1 cut(s) 837
BssMI GATC 7 cut(s) 194, 405, 605, 711, 741, 913, 1007
Bst2UI CCWGG 1 cut(s) 137
Bst4CI ACNGT 1 cut(s) 367
Bst6I CTCTTC 4 cut(s) 761, 1031, 1115, 1205
BstAFI CTTAAG 2 cut(s) 623, 1171
BstC8I GCNNGC 7 cut(s) 61, 110, 167, 210, 214, 1106, 1110
BstDEI CTNAG 2 cut(s) 118, 263
BstF5I GGATG 5 cut(s) 258, 814, 847, 877, 893
BstFNI CGCG 1 cut(s) 427
BstHHI GCGC 2 cut(s) 391, 955
BstKTI GATC 7 cut(s) 197, 408, 608, 714, 744, 916, 1010
BstMAI GTCTC 1 cut(s) 29
BstMBI GATC 7 cut(s) 194, 405, 605, 711, 741, 913, 1007
BstNI CCWGG 1 cut(s) 137
BstNSI RCATGY 1 cut(s) 557
BstSCI CCNGG 2 cut(s) 135, 176
BstUI CGCG 1 cut(s) 427
BstV1I GCAGC 6 cut(s) 68, 81, 90, 99, 310, 313
Bsu15I ATCGAT 1 cut(s) 662
BsuRI GGCC 6 cut(s) 95, 216, 316, 1083, 1093, 1158
BsuTUI ATCGAT 1 cut(s) 662
BtgZI GCGATG 2 cut(s) 354, 1098
BtsCI GGATG 5 cut(s) 258, 814, 847, 877, 893
BtsI GCAGTG 2 cut(s) 461, 972
BtsIMutI CAGTG 4 cut(s) 197, 271, 461, 972
Cac8I GCNNGC 7 cut(s) 61, 110, 167, 210, 214, 1106, 1110
CciI TCATGA 2 cut(s) 495, 673
CfoI GCGC 2 cut(s) 391, 955
Cfr10I RCCGGY 1 cut(s) 212
Cfr13I GGNCC 2 cut(s) 180, 1091
ClaI ATCGAT 1 cut(s) 662
Csp6I GTAC 3 cut(s) 148, 900, 1215
CviAII CATG 5 cut(s) 162, 496, 554, 674, 1085
CviQI GTAC 3 cut(s) 148, 900, 1215
DdeI CTNAG 2 cut(s) 118, 263
DpnI GATC 7 cut(s) 196, 407, 607, 713, 743, 915, 1009
DpnII GATC 7 cut(s) 194, 405, 605, 711, 741, 913, 1007
EaeI YGGCCR 2 cut(s) 214, 1081
Eam1104I CTCTTC 4 cut(s) 761, 1031, 1115, 1205
EarI CTCTTC 4 cut(s) 761, 1031, 1115, 1205
Ecl136II GAGCTC 2 cut(s) 133, 771
Eco147I AGGCCT 3 cut(s) 95, 316, 1158
Eco24I GRGCYC 2 cut(s) 135, 773
Eco32I GATATC 1 cut(s) 142
Eco47I GGWCC 1 cut(s) 180
Eco53kI GAGCTC 2 cut(s) 133, 771
EcoICRI GAGCTC 2 cut(s) 133, 771
EcoO109I RGGNCCY 1 cut(s) 180
EcoRII CCWGG 1 cut(s) 135
EcoRV GATATC 1 cut(s) 142
EcoT38I GRGCYC 2 cut(s) 135, 773
Esp3I CGTCTC 1 cut(s) 29
FaeI CATG 5 cut(s) 165, 499, 557, 677, 1088
FatI CATG 5 cut(s) 161, 495, 553, 673, 1084
FauI CCCGC 1 cut(s) 117
FbaI TGATCA 1 cut(s) 913
Fnu4HI GCNGC 6 cut(s) 57, 70, 79, 88, 299, 302
FokI GGATG 5 cut(s) 245, 821, 854, 884, 900
FriOI GRGCYC 2 cut(s) 135, 773
Fsp4HI GCNGC 6 cut(s) 57, 70, 79, 88, 299, 302
FspBI CTAG 2 cut(s) 903, 1243
GlaI GCGC 2 cut(s) 390, 954
GluI GCNGC 6 cut(s) 57, 70, 79, 88, 299, 302
GsuI CTGGAG 1 cut(s) 119
HaeIII GGCC 6 cut(s) 95, 216, 316, 1083, 1093, 1158
HapII CCGG 2 cut(s) 177, 213
HhaI GCGC 2 cut(s) 391, 955
Hin1II CATG 5 cut(s) 165, 499, 557, 677, 1088
Hin6I GCGC 2 cut(s) 389, 953
HinP1I GCGC 2 cut(s) 389, 953
HincII GTYRAC 1 cut(s) 457
HindII GTYRAC 1 cut(s) 457
HindIII AAGCTT 2 cut(s) 449, 1055
HinfI GANTC 2 cut(s) 4, 659
HpaII CCGG 2 cut(s) 177, 213
HphI GGTGA 4 cut(s) 313, 361, 838, 923
Hpy166II GTNNAC 2 cut(s) 457, 900
Hpy188I TCNGA 8 cut(s) 13, 100, 332, 410, 436, 643, 838, 871
Hpy188III TCNNGA 5 cut(s) 395, 496, 603, 674, 1237
Hpy8I GTNNAC 2 cut(s) 457, 900
HpyAV CCTTC 5 cut(s) 193, 346, 412, 778, 1082
HpyCH4III ACNGT 1 cut(s) 367
HpyCH4IV ACGT 2 cut(s) 22, 124
HpyCH4V TGCA 3 cut(s) 165, 517, 1108
HpyF3I CTNAG 2 cut(s) 118, 263
HpySE526I ACGT 2 cut(s) 22, 124
Hsp92II CATG 5 cut(s) 165, 499, 557, 677, 1088
HspAI GCGC 2 cut(s) 389, 953
KroI GCCGGC 1 cut(s) 212
KroNI GCCGGC 1 cut(s) 214
Ksp22I TGATCA 1 cut(s) 913
Kzo9I GATC 7 cut(s) 194, 405, 605, 711, 741, 913, 1007
LguI GCTCTTC 1 cut(s) 761
LmnI GCTCC 2 cut(s) 138, 776
Lsp1109I GCAGC 6 cut(s) 68, 81, 90, 99, 310, 313
LweI GCATC 5 cut(s) 81, 400, 670, 964, 1121
MaeI CTAG 2 cut(s) 903, 1243
MaeII ACGT 2 cut(s) 22, 124
MaeIII GTNAC 2 cut(s) 31, 361
MalI GATC 7 cut(s) 196, 407, 607, 713, 743, 915, 1009
MboI GATC 7 cut(s) 194, 405, 605, 711, 741, 913, 1007
MboII GAAGA 5 cut(s) 113, 778, 1048, 1132, 1222
MhlI GDGCHC 2 cut(s) 135, 773
MlsI TGGCCA 1 cut(s) 1083
MluCI AATT 3 cut(s) 508, 820, 1065
MluNI TGGCCA 1 cut(s) 1083
MmeI TCCRAC 1 cut(s) 908
Mox20I TGGCCA 1 cut(s) 1083
MroNI GCCGGC 1 cut(s) 212
MscI TGGCCA 1 cut(s) 1083
MseI TTAA 5 cut(s) 447, 624, 666, 687, 1172
MslI CAYNNNNRTG 3 cut(s) 290, 678, 807
Msp20I TGGCCA 1 cut(s) 1083
MspCI CTTAAG 2 cut(s) 623, 1171
MspI CCGG 2 cut(s) 177, 213
MspR9I CCNGG 2 cut(s) 137, 178
MvaI CCWGG 1 cut(s) 137
MvnI CGCG 1 cut(s) 427
NaeI GCCGGC 1 cut(s) 214
NciI CCSGG 1 cut(s) 178
NdeII GATC 7 cut(s) 194, 405, 605, 711, 741, 913, 1007
NgoMIV GCCGGC 1 cut(s) 212
NlaIII CATG 5 cut(s) 165, 499, 557, 677, 1088
NlaIV GGNNCC 2 cut(s) 181, 530
NmuCI GTSAC 1 cut(s) 361
NspI RCATGY 1 cut(s) 557
PagI TCATGA 2 cut(s) 495, 673
PceI AGGCCT 3 cut(s) 95, 316, 1158
PciSI GCTCTTC 1 cut(s) 761
PcsI WCGNNNNNNNCGW 1 cut(s) 336
PdiI GCCGGC 1 cut(s) 214
PfeI GAWTC 1 cut(s) 659
PfoI TCCNGGA 1 cut(s) 135
PkrI GCNGC 6 cut(s) 58, 71, 80, 89, 300, 303
PpuMI RGGWCCY 1 cut(s) 180
PshBI ATTAAT 1 cut(s) 687
Psp124BI GAGCTC 2 cut(s) 135, 773
Psp5II RGGWCCY 1 cut(s) 180
Psp6I CCWGG 1 cut(s) 135
PspGI CCWGG 1 cut(s) 135
PspN4I GGNNCC 2 cut(s) 181, 530
PspPI GGNCC 2 cut(s) 180, 1091
PspPPI RGGWCCY 1 cut(s) 180
RsaI GTAC 3 cut(s) 149, 901, 1216
RsaNI GTAC 3 cut(s) 148, 900, 1215
RseI CAYNNNNRTG 3 cut(s) 290, 678, 807
SacI GAGCTC 2 cut(s) 135, 773
SapI GCTCTTC 1 cut(s) 761
SaqAI TTAA 5 cut(s) 447, 624, 666, 687, 1172
SatI GCNGC 6 cut(s) 57, 70, 79, 88, 299, 302
Sau3AI GATC 7 cut(s) 194, 405, 605, 711, 741, 913, 1007
Sau96I GGNCC 2 cut(s) 180, 1091
ScrFI CCNGG 2 cut(s) 137, 178
SduI GDGCHC 2 cut(s) 135, 773
SfaNI GCATC 5 cut(s) 81, 400, 670, 964, 1121
SinI GGWCC 1 cut(s) 180
SmiMI CAYNNNNRTG 3 cut(s) 290, 678, 807
SmlI CTYRAG 3 cut(s) 623, 1171, 1237
SmoI CTYRAG 3 cut(s) 623, 1171, 1237
SpeI ACTAGT 1 cut(s) 902
Sse9I AATT 3 cut(s) 508, 820, 1065
SseBI AGGCCT 3 cut(s) 95, 316, 1158
SsiI CCGC 4 cut(s) 110, 356, 427, 864
SspMI CTAG 2 cut(s) 903, 1243
SstI GAGCTC 2 cut(s) 135, 773
StuI AGGCCT 3 cut(s) 95, 316, 1158
StyD4I CCNGG 2 cut(s) 135, 176
TaaI ACNGT 1 cut(s) 367
TaiI ACGT 2 cut(s) 25, 127
TaqI TCGA 3 cut(s) 129, 227, 662
TasI AATT 3 cut(s) 508, 820, 1065
TatI WGTACW 2 cut(s) 899, 1214
TfiI GAWTC 1 cut(s) 659
Tru1I TTAA 5 cut(s) 447, 624, 666, 687, 1172
Tru9I TTAA 5 cut(s) 447, 624, 666, 687, 1172
TscAI CASTG 4 cut(s) 204, 271, 468, 972
TseFI GTSAC 1 cut(s) 361
TseI GCWGC 6 cut(s) 56, 69, 78, 87, 298, 301
Tsp45I GTSAC 1 cut(s) 361
TspDTI ATGAA 4 cut(s) 247, 484, 512, 1101
TspRI CASTG 4 cut(s) 204, 271, 468, 972
Vha464I CTTAAG 2 cut(s) 623, 1171
VpaK11BI GGWCC 1 cut(s) 180
VspI ATTAAT 1 cut(s) 687
XceI RCATGY 1 cut(s) 557
XcmI CCANNNNNNNNNTGG 1 cut(s) 691
XspI CTAG 2 cut(s) 903, 1243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.