Rh1AG040100

Myosin heavy chain kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0014503
Physical Location & Seq
Reverse (-)
7082556 .. 7084341
1786 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG040100.1

Sequence Viewer

Length: 1275 bp
ATGTTTCATCCCATGACTCTTTCAGAAAACAAACGCCTCTCAGTAACTCCCTTACTCTCTGCCACAGCAGCAAGCACCAGCAGCACAAGCAGCGAGGCCTCCGAAGAAAGCCCGCCTACCTCAGCACGTTTCGAGCTCCAGGATATCAAGTACCATCATTTTCCATGCACGCACTTATCCGGGTCCTTCTCCTACCGATCACTGGCTGTGCTTGCCGGCCATAAAGGCTCGATTTCGTGCCTGTCCTTATGTGGAGAGTTCATTCTCAGTGCTTCACAAGGCAAAGACATCATCGTGTGGCAGCAGCCTGATTTGAGGCCTTTCACCAAGTTCGGACAAGGCGATGGCTCGGTGAAGGCGGTTGTGACCGTTGGGAACAAGGTCTTTACGGCGCATCAAGACAGCAGGATCAGAGTTTGGAAGGTTTCGCGGAGTTCAGAGAATGTGTTTAAGCTTGTGGACACACTGCCTACAACGAAAGACTATTTGGGGAAGTTCATGAAGCAAAGCAATTATGTGCAAACGAGGAGGCACCACAAGAAGTTGTGGATTGAACATGCCGATAGTATCTCTTGTCTGGCTTTCTATAATGGGTTGATTTACTCTGGATCGTGGGATAAGACCCTTAAGGTGTGGCGAGTTTCGGATTTGAAGTGCGTGGAGTCGATTAAGGCTCATGATGATGCCATTAATGGGTTGGTGGCAAGTAAAGGGATCGTGTATTCTGCTTCTGCCGATGGAAAGATCAAGGCTTGGGGAAAAGAGGGGAAGAGCTCCCATTGCTTGAAGGGTATTTTGGAGGGTCATAAGGATGTTTCGTTCAATTCGGTGATTGTTTCAGAGGATGGGAGATGGGTTTATGGAGGCGGTTCCGATGGGTTTGTGATGGGATGGGAAGTGTGTACTAGTGGTGGTGATCAAGTTGCGAGCTGGAAGTTGGTTTCCGAAACAAAAGCGCATCAAATGGCAGTGTTGTCAATGTGTTTGATGGGGGAGATGTTGTGTAGTGGATCAGCAGATAAGAGTATTGGTATATGGAAGAGAGAGGCTTTTGGGAAGCTTTCCAAAATTGGGGTTATTAGCGGCCATGAAGGGCCAGTCAAGTGCTTGCAAGCATCGCCGAATAGTGTTGGTGGTGGTTTTATGTTGTATAGTGGAGGCCTTGACAAGACCTTAAGAGTCTGGTGGGTTTCCAAGCCCTCCACAACACAGAAGAGTACAGAGGAAAAGTCCATCATCTTGAGCGAGAAATTCTACAAAGTCTCTGTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

424

Amino Acids

46.38

Weight (kDa)

9.1

Isoelectric Point (pI)

39.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_SCAP PF24017 65 - 252 3e-07 SCAP Beta-propeller
Beta-prop_WDR5 PF25175 71 - 273 6.1e-18 WDR5 beta-propeller domain
WDR55 PF24796 172 - 372 6.7e-09 WDR55
Beta-prop_TEP1_2nd PF25047 183 - 257 3e-06 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 184 - 300 3.8e-11 WDR3 first beta-propeller domain
WD40_CDC20-Fz PF24807 185 - 324 6.8e-07 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 189 - 407 3.3e-17 Prp19 WD40 domain
Beta-prop_WDR3_2nd PF25172 197 - 299 2.2e-08 WDR3 second beta-propeller domain
Beta-prop_THOC3 PF25174 198 - 303 9.1e-08 THOC3 beta-propeller domain
Beta-prop_EML PF23409 249 - 372 4.1e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR5 PF25175 267 - 405 9.5e-11 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 287 - 402 4.6e-11 TEP-1 second beta-propeller
WD40_CDC20-Fz PF24807 311 - 413 3.1e-07 CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st PF25173 312 - 415 3.9e-07 WDR3 first beta-propeller domain
Beta-prop_THOC3 PF25174 325 - 411 1.4e-08 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014503)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51930
fragaria_vesca FvH4_7g02030
malus_domestica MD02G1296500.v1.1 MD07G1027800.v1.1
prunus_persica Prupe.2G027900_v2.0.a1 Prupe.2G027900_v2.0.a1
pyrus_communis pycom02g24950 pycom07g02110
rosa_chinensis RchiOBHm_Chr1g0319521
rosa_laevigata RLG00000030475
rosa_multiflora Rmu_sc0002539.1_g000012
rosa_roxburghii Rroxscaffold_4G00328310
rosa_samantha Rh1AG040100 Rh1BG037000 Rh1CG041100 Rh1DG028800
rosa_wichuraiana Rw1G003240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 531
AccII CGCG 1 cut(s) 430
AciI CCGC 5 cut(s) 113, 359, 430, 867, 1083
AclWI GGATC 4 cut(s) 416, 616, 722, 1018
AcoI YGGCCR 2 cut(s) 217, 1084
AcsI RAATTY 1 cut(s) 1250
AfaI GTAC 3 cut(s) 152, 904, 1219
AfiI CCNNNNNNNGG 3 cut(s) 202, 693, 1071
AflII CTTAAG 2 cut(s) 626, 1174
AgsI TTSAA 4 cut(s) 554, 652, 787, 823
AhlI ACTAGT 1 cut(s) 905
AjnI CCWGG 1 cut(s) 138
AjuI GAANNNNNNNTTGG 4 cut(s) 470, 502, 779, 811
AloI GAACNNNNNNTCC 2 cut(s) 803, 835
AluBI AGCT 5 cut(s) 136, 454, 774, 930, 1060
AluI AGCT 5 cut(s) 136, 454, 774, 930, 1060
Alw21I GWGCWC 2 cut(s) 138, 776
Alw26I GTCTC 1 cut(s) 1267
AlwI GGATC 4 cut(s) 416, 616, 722, 1018
AoxI GGCC 6 cut(s) 96, 217, 317, 1084, 1094, 1159
ApeKI GCWGC 5 cut(s) 68, 81, 90, 301, 304
ApoI RAATTY 1 cut(s) 1250
AseI ATTAAT 1 cut(s) 690
AspLEI GCGC 2 cut(s) 394, 958
AspS9I GGNCC 2 cut(s) 183, 1094
AsuC2I CCSGG 1 cut(s) 181
AsuHPI GGTGA 4 cut(s) 316, 364, 841, 926
AvaII GGWCC 1 cut(s) 183
BanI GGYRCC 1 cut(s) 531
BanII GRGCYC 2 cut(s) 138, 776
Bbv12I GWGCWC 2 cut(s) 138, 776
BbvCI CCTCAGC 1 cut(s) 121
BbvI GCAGC 5 cut(s) 80, 93, 102, 313, 316
BceAI ACGGC 1 cut(s) 405
BciT130I CCWGG 1 cut(s) 140
BclI TGATCA 1 cut(s) 916
BcnI CCSGG 1 cut(s) 181
BcoDI GTCTC 1 cut(s) 1267
BcuI ACTAGT 1 cut(s) 905
BfaI CTAG 1 cut(s) 906
BfrI CTTAAG 2 cut(s) 626, 1174
BglI GCCNNNNNGGC 1 cut(s) 225
BisI GCNGC 6 cut(s) 69, 82, 91, 302, 305, 1084
BlsI GCNGC 6 cut(s) 70, 83, 92, 303, 306, 1085
Bme1390I CCNGG 2 cut(s) 140, 181
Bme18I GGWCC 1 cut(s) 183
BmgT120I GGNCC 2 cut(s) 183, 1094
BmiI GGNNCC 3 cut(s) 184, 533, 871
BmrFI CCNGG 2 cut(s) 140, 181
BmsI GCATC 4 cut(s) 403, 673, 967, 1124
BpmI CTGGAG 1 cut(s) 122
Bpu10I CCTNAGC 1 cut(s) 121
BpuEI CTTGAG 1 cut(s) 1261
BpuMI CCSGG 1 cut(s) 181
BsaXI ACNNNNNCTCC 2 cut(s) 986, 1016
Bsc4I CCNNNNNNNGG 3 cut(s) 202, 693, 1071
Bse118I RCCGGY 1 cut(s) 215
Bse1I ACTGG 2 cut(s) 207, 1097
Bse3DI GCAATG 1 cut(s) 778
BseBI CCWGG 1 cut(s) 140
BseGI GGATG 4 cut(s) 7, 817, 850, 896
BseLI CCNNNNNNNGG 3 cut(s) 202, 693, 1071
BseMI GCAATG 1 cut(s) 778
BseMII CTCAG 3 cut(s) 54, 135, 280
BseNI ACTGG 2 cut(s) 207, 1097
BseRI GAGGAG 1 cut(s) 541
BseXI GCAGC 5 cut(s) 80, 93, 102, 313, 316
Bsh1236I CGCG 1 cut(s) 430
BshFI GGCC 6 cut(s) 98, 219, 319, 1086, 1096, 1161
BshNI GGYRCC 1 cut(s) 531
BsiHKAI GWGCWC 2 cut(s) 138, 776
BsiSI CCGG 2 cut(s) 180, 216
BslI CCNNNNNNNGG 3 cut(s) 202, 693, 1071
BsmAI GTCTC 1 cut(s) 1267
BsnI GGCC 6 cut(s) 98, 219, 319, 1086, 1096, 1161
Bsp1286I GDGCHC 2 cut(s) 138, 776
Bsp143I GATC 7 cut(s) 197, 408, 608, 714, 744, 916, 1010
BspACI CCGC 5 cut(s) 113, 359, 430, 867, 1083
BspANI GGCC 6 cut(s) 98, 219, 319, 1086, 1096, 1161
BspCNI CTCAG 3 cut(s) 53, 134, 279
BspFNI CGCG 1 cut(s) 430
BspHI TCATGA 2 cut(s) 498, 676
BspLI GGNNCC 3 cut(s) 184, 533, 871
BspPI GGATC 4 cut(s) 416, 616, 722, 1018
BspQI GCTCTTC 1 cut(s) 764
BspT107I GGYRCC 1 cut(s) 531
BspTI CTTAAG 2 cut(s) 626, 1174
BsrDI GCAATG 1 cut(s) 778
BsrFI RCCGGY 1 cut(s) 215
BsrI ACTGG 2 cut(s) 207, 1097
BssAI RCCGGY 1 cut(s) 215
BssMI GATC 7 cut(s) 197, 408, 608, 714, 744, 916, 1010
Bst2UI CCWGG 1 cut(s) 140
Bst4CI ACNGT 1 cut(s) 370
Bst6I CTCTTC 3 cut(s) 764, 1034, 1208
BstAFI CTTAAG 2 cut(s) 626, 1174
BstC8I GCNNGC 8 cut(s) 73, 113, 170, 213, 217, 928, 1109, 1113
BstDEI CTNAG 3 cut(s) 40, 121, 266
BstF5I GGATG 4 cut(s) 7, 817, 850, 896
BstFNI CGCG 1 cut(s) 430
BstHHI GCGC 2 cut(s) 394, 958
BstKTI GATC 7 cut(s) 200, 411, 611, 717, 747, 919, 1013
BstMAI GTCTC 1 cut(s) 1267
BstMBI GATC 7 cut(s) 197, 408, 608, 714, 744, 916, 1010
BstMWI GCNNNNNNNGC 8 cut(s) 68, 81, 87, 90, 212, 225, 780, 1117
BstNI CCWGG 1 cut(s) 140
BstNSI RCATGY 1 cut(s) 560
BstSCI CCNGG 2 cut(s) 138, 179
BstUI CGCG 1 cut(s) 430
BstV1I GCAGC 5 cut(s) 80, 93, 102, 313, 316
BsuRI GGCC 6 cut(s) 98, 219, 319, 1086, 1096, 1161
BtgZI GCGATG 2 cut(s) 357, 1101
BtsCI GGATG 4 cut(s) 7, 817, 850, 896
BtsI GCAGTG 2 cut(s) 464, 975
BtsIMutI CAGTG 4 cut(s) 200, 274, 464, 975
Cac8I GCNNGC 8 cut(s) 73, 113, 170, 213, 217, 928, 1109, 1113
CciI TCATGA 2 cut(s) 498, 676
CfoI GCGC 2 cut(s) 394, 958
Cfr10I RCCGGY 1 cut(s) 215
Cfr13I GGNCC 2 cut(s) 183, 1094
Csp6I GTAC 3 cut(s) 151, 903, 1218
CviAII CATG 6 cut(s) 13, 165, 499, 557, 677, 1088
CviQI GTAC 3 cut(s) 151, 903, 1218
DdeI CTNAG 3 cut(s) 40, 121, 266
DpnI GATC 7 cut(s) 199, 410, 610, 716, 746, 918, 1012
DpnII GATC 7 cut(s) 197, 408, 608, 714, 744, 916, 1010
EaeI YGGCCR 2 cut(s) 217, 1084
Eam1104I CTCTTC 3 cut(s) 764, 1034, 1208
EarI CTCTTC 3 cut(s) 764, 1034, 1208
Ecl136II GAGCTC 2 cut(s) 136, 774
Eco147I AGGCCT 3 cut(s) 98, 319, 1161
Eco24I GRGCYC 2 cut(s) 138, 776
Eco32I GATATC 1 cut(s) 145
Eco47I GGWCC 1 cut(s) 183
Eco53kI GAGCTC 2 cut(s) 136, 774
EcoICRI GAGCTC 2 cut(s) 136, 774
EcoO109I RGGNCCY 1 cut(s) 183
EcoRII CCWGG 1 cut(s) 138
EcoRV GATATC 1 cut(s) 145
EcoT38I GRGCYC 2 cut(s) 138, 776
FaeI CATG 6 cut(s) 16, 168, 502, 560, 680, 1091
FatI CATG 6 cut(s) 12, 164, 498, 556, 676, 1087
FauI CCCGC 1 cut(s) 120
FbaI TGATCA 1 cut(s) 916
Fnu4HI GCNGC 6 cut(s) 69, 82, 91, 302, 305, 1084
FokI GGATG 3 cut(s) 824, 857, 903
FriOI GRGCYC 2 cut(s) 138, 776
Fsp4HI GCNGC 6 cut(s) 69, 82, 91, 302, 305, 1084
FspBI CTAG 1 cut(s) 906
GlaI GCGC 2 cut(s) 393, 957
GluI GCNGC 6 cut(s) 69, 82, 91, 302, 305, 1084
GsuI CTGGAG 1 cut(s) 122
HaeIII GGCC 6 cut(s) 98, 219, 319, 1086, 1096, 1161
HapII CCGG 2 cut(s) 180, 216
HhaI GCGC 2 cut(s) 394, 958
Hin1II CATG 6 cut(s) 16, 168, 502, 560, 680, 1091
Hin6I GCGC 2 cut(s) 392, 956
HinP1I GCGC 2 cut(s) 392, 956
HindIII AAGCTT 2 cut(s) 452, 1058
HinfI GANTC 3 cut(s) 16, 662, 1179
HpaII CCGG 2 cut(s) 180, 216
HphI GGTGA 4 cut(s) 316, 364, 841, 926
Hpy166II GTNNAC 2 cut(s) 460, 903
Hpy188I TCNGA 9 cut(s) 25, 103, 335, 413, 439, 646, 841, 874, 946
Hpy188III TCNNGA 5 cut(s) 398, 499, 606, 677, 1240
Hpy8I GTNNAC 2 cut(s) 460, 903
HpyAV CCTTC 5 cut(s) 196, 349, 415, 781, 1085
HpyCH4III ACNGT 1 cut(s) 370
HpyCH4IV ACGT 1 cut(s) 127
HpyCH4V TGCA 3 cut(s) 168, 520, 1111
HpyF10VI GCNNNNNNNGC 8 cut(s) 68, 81, 87, 90, 212, 225, 780, 1117
HpyF3I CTNAG 3 cut(s) 40, 121, 266
HpySE526I ACGT 1 cut(s) 127
Hsp92II CATG 6 cut(s) 16, 168, 502, 560, 680, 1091
HspAI GCGC 2 cut(s) 392, 956
KroI GCCGGC 1 cut(s) 215
KroNI GCCGGC 1 cut(s) 217
Ksp22I TGATCA 1 cut(s) 916
Kzo9I GATC 7 cut(s) 197, 408, 608, 714, 744, 916, 1010
LguI GCTCTTC 1 cut(s) 764
LmnI GCTCC 2 cut(s) 141, 779
Lsp1109I GCAGC 5 cut(s) 80, 93, 102, 313, 316
LweI GCATC 4 cut(s) 403, 673, 967, 1124
MaeI CTAG 1 cut(s) 906
MaeII ACGT 1 cut(s) 127
MaeIII GTNAC 2 cut(s) 43, 364
MalI GATC 7 cut(s) 199, 410, 610, 716, 746, 918, 1012
MboI GATC 7 cut(s) 197, 408, 608, 714, 744, 916, 1010
MboII GAAGA 4 cut(s) 116, 781, 1051, 1225
MhlI GDGCHC 2 cut(s) 138, 776
MluCI AATT 4 cut(s) 511, 823, 1068, 1250
MlyI GAGTC 3 cut(s) 10, 671, 1188
MroNI GCCGGC 1 cut(s) 215
MseI TTAA 5 cut(s) 450, 627, 669, 690, 1175
MslI CAYNNNNRTG 3 cut(s) 293, 681, 810
MspCI CTTAAG 2 cut(s) 626, 1174
MspI CCGG 2 cut(s) 180, 216
MspR9I CCNGG 2 cut(s) 140, 181
MvaI CCWGG 1 cut(s) 140
MvnI CGCG 1 cut(s) 430
MwoI GCNNNNNNNGC 8 cut(s) 68, 81, 87, 90, 212, 225, 780, 1117
NaeI GCCGGC 1 cut(s) 217
NciI CCSGG 1 cut(s) 181
NdeII GATC 7 cut(s) 197, 408, 608, 714, 744, 916, 1010
NgoMIV GCCGGC 1 cut(s) 215
NlaIII CATG 6 cut(s) 16, 168, 502, 560, 680, 1091
NlaIV GGNNCC 3 cut(s) 184, 533, 871
NmuCI GTSAC 1 cut(s) 364
NspI RCATGY 1 cut(s) 560
PagI TCATGA 2 cut(s) 498, 676
PceI AGGCCT 3 cut(s) 98, 319, 1161
PciSI GCTCTTC 1 cut(s) 764
PcsI WCGNNNNNNNCGW 1 cut(s) 339
PdiI GCCGGC 1 cut(s) 217
PfoI TCCNGGA 1 cut(s) 138
PkrI GCNGC 6 cut(s) 70, 83, 92, 303, 306, 1085
PleI GAGTC 3 cut(s) 10, 670, 1187
PpsI GAGTC 3 cut(s) 10, 670, 1187
PpuMI RGGWCCY 1 cut(s) 183
PshBI ATTAAT 1 cut(s) 690
Psp124BI GAGCTC 2 cut(s) 138, 776
Psp5II RGGWCCY 1 cut(s) 183
Psp6I CCWGG 1 cut(s) 138
PspGI CCWGG 1 cut(s) 138
PspN4I GGNNCC 3 cut(s) 184, 533, 871
PspPI GGNCC 2 cut(s) 183, 1094
PspPPI RGGWCCY 1 cut(s) 183
RsaI GTAC 3 cut(s) 152, 904, 1219
RsaNI GTAC 3 cut(s) 151, 903, 1218
RseI CAYNNNNRTG 3 cut(s) 293, 681, 810
SacI GAGCTC 2 cut(s) 138, 776
SapI GCTCTTC 1 cut(s) 764
SaqAI TTAA 5 cut(s) 450, 627, 669, 690, 1175
SatI GCNGC 6 cut(s) 69, 82, 91, 302, 305, 1084
Sau3AI GATC 7 cut(s) 197, 408, 608, 714, 744, 916, 1010
Sau96I GGNCC 2 cut(s) 183, 1094
SchI GAGTC 3 cut(s) 10, 671, 1188
ScrFI CCNGG 2 cut(s) 140, 181
SduI GDGCHC 2 cut(s) 138, 776
SfaNI GCATC 4 cut(s) 403, 673, 967, 1124
SinI GGWCC 1 cut(s) 183
SmiMI CAYNNNNRTG 3 cut(s) 293, 681, 810
SmlI CTYRAG 3 cut(s) 626, 1174, 1240
SmoI CTYRAG 3 cut(s) 626, 1174, 1240
SpeI ACTAGT 1 cut(s) 905
Sse9I AATT 4 cut(s) 511, 823, 1068, 1250
SseBI AGGCCT 3 cut(s) 98, 319, 1161
SsiI CCGC 5 cut(s) 113, 359, 430, 867, 1083
SspMI CTAG 1 cut(s) 906
SstI GAGCTC 2 cut(s) 138, 776
StuI AGGCCT 3 cut(s) 98, 319, 1161
StyD4I CCNGG 2 cut(s) 138, 179
TaaI ACNGT 1 cut(s) 370
TaiI ACGT 1 cut(s) 130
TaqI TCGA 3 cut(s) 132, 230, 665
TasI AATT 4 cut(s) 511, 823, 1068, 1250
TatI WGTACW 2 cut(s) 902, 1217
TauI GCSGC 1 cut(s) 1086
Tru1I TTAA 5 cut(s) 450, 627, 669, 690, 1175
Tru9I TTAA 5 cut(s) 450, 627, 669, 690, 1175
TscAI CASTG 4 cut(s) 207, 274, 471, 975
TseFI GTSAC 1 cut(s) 364
TseI GCWGC 5 cut(s) 68, 81, 90, 301, 304
Tsp45I GTSAC 1 cut(s) 364
TspDTI ATGAA 4 cut(s) 250, 487, 515, 1104
TspRI CASTG 4 cut(s) 207, 274, 471, 975
Vha464I CTTAAG 2 cut(s) 626, 1174
VpaK11BI GGWCC 1 cut(s) 183
VspI ATTAAT 1 cut(s) 690
XapI RAATTY 1 cut(s) 1250
XceI RCATGY 1 cut(s) 560
XcmI CCANNNNNNNNNTGG 1 cut(s) 694
XspI CTAG 1 cut(s) 906
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.