Prupe.2G027900_v2.0.a1

Myosin heavy chain kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0014503
Physical Location & Seq
Reverse (-)
2894674 .. 2896536
1863 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G027900.2

Sequence Viewer

Length: 1281 bp
ATGTTTCATCAAATCAGCTCCACTCTATTGGAAAACAAACGCCTCTCAATTCCTTTGCTCTCTGCCTCAGCAAGAAGCCCTACTAGCAGCATTAGCAGCAACAGCAGAAGCACAAGCAGCGAGGTAGATGAAAGCCCGCGGGATTCAGGCCATTTCGAGCTCCAAGATCATGTCAAGTGTCAATCTCCATGTGTACCCTTTTCGGGGTCTTTCTCGTATCGATCATTGGCGGTGCTCTCTGGCCATGTAGGCTCAGTTTCATGCTTGGCCTTATGTGGGGAATTCATTCTCAGTGCTTCACAAGGCAAAGACATCATAGTGTGGCAACAGCCTGATTTGAGGCCTTTCACCAAGTTTGGACAAGGTGATGGCTCTGTCAAGGCACTTGTCACCGTTGGGAACAAGGTCTTTACAGCTCACCAGGATAGCAGGATTAGAGTTTGGAAGGTTTCACGGCGATCGGAGAATGTGTTTAGGCTTGTTGATACACTTCCCACAACTAAAGATTATTTGGGGAAGTTCATGAAGCAGAGCAATTATGTCCAAATTCGGAGACATCACAAGCGATTGTGGATCGAACACGCCGATAGTATATCTTGTTTGGCCTTCTATAACGGCTTGATTTACTCTGGATCATGGGATAAGACTCTTAAGGTGTGGAGGATCTCGGATTTGAAGTGCATAGAATCCATCAAGGCTCATGATGATGCTATTAATGGGCTGGTGGCATGTAATGGGATTGTGTATTCTGCTTCTGCAGATGGAAAAATTAAAGCTTGGGGAAAAGAGGGGAAGAGCTCACATTGTTTGAAGGGTATTTTGGAGGGTCATAAGGATGTTTCACTTAATTCTGTGATCGTTTCTGAGGATGGTAGATGGGTTTATGGAGGCTGTTCGGATGGGTTTGTGATGGGATGGCAAGGGAGTTGTGGTGATGTTCAAAATCAAATTGAGAGCTGGAAGTTGGTTTGTGAAACAAAAGCACATCAAATGGCTGTTTTGTCTATGTGTTTGATGGGGGAGATGTTGTGCAGTGGCTCAGCTGATAAGAGCATTGGCATATGGAAGAGAGAGGCTTTTGGCAAGCTTTGTAAAGTTGGGATCATTAGTGGCCATGAGGGGCCAGTTAAGTGCTTGCAGGCATCACCAAATAATGTTGGTGGAGGTTTCATGTTGTATAGTGGAGGCCTTGACAAGAGCTTAAGGGTTTGGTGGGTTCCCAAGCCCTCTAATAATAGAGCAGAGGAAACCACAGAGGAAAAGTCCATCATCTTATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

427

Amino Acids

46.8

Weight (kDa)

8.76

Isoelectric Point (pI)

47.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014503)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G51930
fragaria_vesca FvH4_7g02030
malus_domestica MD02G1296500.v1.1 MD07G1027800.v1.1
prunus_persica Prupe.2G027900_v2.0.a1 Prupe.2G027900_v2.0.a1
pyrus_communis pycom02g24950 pycom07g02110
rosa_chinensis RchiOBHm_Chr1g0319521
rosa_laevigata RLG00000030475
rosa_multiflora Rmu_sc0002539.1_g000012
rosa_roxburghii Rroxscaffold_4G00328310
rosa_samantha Rh1AG040100 Rh1BG037000 Rh1CG041100 Rh1DG028800
rosa_wichuraiana Rw1G003240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 139
AciI CCGC 3 cut(s) 137, 139, 230
AclWI GGATC 4 cut(s) 581, 640, 671, 1109
AcoI YGGCCR 2 cut(s) 241, 1111
AcsI RAATTY 2 cut(s) 281, 546
AfaI GTAC 1 cut(s) 195
AfiI CCNNNNNNNGG 3 cut(s) 203, 204, 276
AflII CTTAAG 2 cut(s) 650, 1201
AgsI TTSAA 3 cut(s) 676, 811, 941
AjnI CCWGG 1 cut(s) 420
AjuI GAANNNNNNNTTGG 2 cut(s) 803, 835
AluBI AGCT 9 cut(s) 18, 160, 416, 776, 798, 957, 1043, 1087, 1200
AluI AGCT 9 cut(s) 18, 160, 416, 776, 798, 957, 1043, 1087, 1200
Alw21I GWGCWC 3 cut(s) 162, 237, 800
Alw26I GTCTC 1 cut(s) 547
AlwI GGATC 4 cut(s) 581, 640, 671, 1109
AoxI GGCC 8 cut(s) 148, 241, 267, 341, 603, 1111, 1121, 1186
ApeKI GCWGC 3 cut(s) 87, 96, 117
ApoI RAATTY 2 cut(s) 281, 546
AseI ATTAAT 1 cut(s) 714
Asp700I GAANNNNTTC 1 cut(s) 285
AspS9I GGNCC 1 cut(s) 1121
AsuHPI GGTGA 6 cut(s) 340, 377, 382, 410, 944, 1137
BalI TGGCCA 2 cut(s) 243, 1113
BanII GRGCYC 2 cut(s) 162, 800
Bbv12I GWGCWC 3 cut(s) 162, 237, 800
BbvCI CCTCAGC 1 cut(s) 67
BbvI GCAGC 3 cut(s) 99, 108, 129
BceAI ACGGC 2 cut(s) 470, 631
BciT130I CCWGG 1 cut(s) 422
BcoDI GTCTC 1 cut(s) 547
BfaI CTAG 2 cut(s) 84, 1279
BfmI CTRYAG 1 cut(s) 756
BfrI CTTAAG 2 cut(s) 650, 1201
BglI GCCNNNNNGGC 1 cut(s) 249
BisI GCNGC 3 cut(s) 88, 97, 118
BlpI GCTNAGC 1 cut(s) 1039
BlsI GCNGC 3 cut(s) 89, 98, 119
Bme1390I CCNGG 1 cut(s) 422
BmgT120I GGNCC 1 cut(s) 1121
BmiI GGNNCC 2 cut(s) 1122, 1218
BmrFI CCNGG 1 cut(s) 422
BmsI GCATC 2 cut(s) 697, 1151
Bpu10I CCTNAGC 1 cut(s) 67
Bpu1102I GCTNAGC 1 cut(s) 1039
Bsa29I ATCGAT 1 cut(s) 220
BsaJI CCNNGG 1 cut(s) 137
BsaXI ACNNNNNCTCC 6 cut(s) 455, 485, 916, 946, 1013, 1043
Bsc4I CCNNNNNNNGG 3 cut(s) 203, 204, 276
Bse1I ACTGG 1 cut(s) 1124
BseBI CCWGG 1 cut(s) 422
BseCI ATCGAT 1 cut(s) 220
BseDI CCNNGG 1 cut(s) 137
BseGI GGATG 4 cut(s) 841, 874, 904, 920
BseLI CCNNNNNNNGG 3 cut(s) 203, 204, 276
BseMII CTCAG 5 cut(s) 81, 267, 304, 855, 1053
BseNI ACTGG 1 cut(s) 1124
BseXI GCAGC 3 cut(s) 99, 108, 129
BsgI GTGCAG 1 cut(s) 1051
Bsh1236I CGCG 1 cut(s) 139
Bsh1285I CGRYCG 1 cut(s) 461
BshFI GGCC 8 cut(s) 150, 243, 269, 343, 605, 1113, 1123, 1188
BshVI ATCGAT 1 cut(s) 220
BsiEI CGRYCG 1 cut(s) 461
BsiHKAI GWGCWC 3 cut(s) 162, 237, 800
BslI CCNNNNNNNGG 3 cut(s) 203, 204, 276
BsmAI GTCTC 1 cut(s) 547
BsnI GGCC 8 cut(s) 150, 243, 269, 343, 605, 1113, 1123, 1188
Bsp1286I GDGCHC 3 cut(s) 162, 237, 800
Bsp143I GATC 8 cut(s) 166, 221, 458, 573, 632, 663, 855, 1101
Bsp1720I GCTNAGC 1 cut(s) 1039
BspACI CCGC 3 cut(s) 137, 139, 230
BspANI GGCC 8 cut(s) 150, 243, 269, 343, 605, 1113, 1123, 1188
BspCNI CTCAG 5 cut(s) 80, 266, 303, 856, 1052
BspDI ATCGAT 1 cut(s) 220
BspFNI CGCG 1 cut(s) 139
BspHI TCATGA 2 cut(s) 522, 700
BspLI GGNNCC 2 cut(s) 1122, 1218
BspMAI CTGCAG 1 cut(s) 760
BspPI GGATC 4 cut(s) 581, 640, 671, 1109
BspQI GCTCTTC 1 cut(s) 788
BspTI CTTAAG 2 cut(s) 650, 1201
BsrI ACTGG 1 cut(s) 1124
BssECI CCNNGG 1 cut(s) 137
BssMI GATC 8 cut(s) 166, 221, 458, 573, 632, 663, 855, 1101
Bst2UI CCWGG 1 cut(s) 422
Bst4CI ACNGT 1 cut(s) 394
Bst6I CTCTTC 2 cut(s) 788, 1061
BstAFI CTTAAG 2 cut(s) 650, 1201
BstC8I GCNNGC 4 cut(s) 137, 1085, 1136, 1140
BstDEI CTNAG 5 cut(s) 67, 253, 290, 864, 1039
BstDSI CCRYGG 1 cut(s) 137
BstF5I GGATG 4 cut(s) 841, 874, 904, 920
BstFNI CGCG 1 cut(s) 139
BstKTI GATC 8 cut(s) 169, 224, 461, 576, 635, 666, 858, 1104
BstMAI GTCTC 1 cut(s) 547
BstMBI GATC 8 cut(s) 166, 221, 458, 573, 632, 663, 855, 1101
BstMCI CGRYCG 1 cut(s) 461
BstMWI GCNNNNNNNGC 6 cut(s) 84, 93, 96, 102, 117, 249
BstNI CCWGG 1 cut(s) 422
BstNSI RCATGY 1 cut(s) 732
BstSCI CCNGG 1 cut(s) 420
BstSFI CTRYAG 1 cut(s) 756
BstUI CGCG 1 cut(s) 139
BstV1I GCAGC 3 cut(s) 99, 108, 129
BstX2I RGATCY 1 cut(s) 663
BstXI CCANNNNNNTGG 1 cut(s) 28
BstYI RGATCY 1 cut(s) 663
Bsu15I ATCGAT 1 cut(s) 220
BsuRI GGCC 8 cut(s) 150, 243, 269, 343, 605, 1113, 1123, 1188
BsuTUI ATCGAT 1 cut(s) 220
BtgI CCRYGG 1 cut(s) 137
BtsCI GGATG 4 cut(s) 841, 874, 904, 920
BtsI GCAGTG 1 cut(s) 1039
BtsIMutI CAGTG 2 cut(s) 298, 1039
Cac8I GCNNGC 4 cut(s) 137, 1085, 1136, 1140
CciI TCATGA 2 cut(s) 522, 700
Cfr13I GGNCC 1 cut(s) 1121
Cfr42I CCGCGG 1 cut(s) 140
ClaI ATCGAT 1 cut(s) 220
Csp6I GTAC 1 cut(s) 194
CviQI GTAC 1 cut(s) 194
DdeI CTNAG 5 cut(s) 67, 253, 290, 864, 1039
DpnI GATC 8 cut(s) 168, 223, 460, 575, 634, 665, 857, 1103
DpnII GATC 8 cut(s) 166, 221, 458, 573, 632, 663, 855, 1101
EaeI YGGCCR 2 cut(s) 241, 1111
Eam1104I CTCTTC 2 cut(s) 788, 1061
EarI CTCTTC 2 cut(s) 788, 1061
Ecl136II GAGCTC 2 cut(s) 160, 798
Eco147I AGGCCT 2 cut(s) 343, 1188
Eco24I GRGCYC 2 cut(s) 162, 800
Eco53kI GAGCTC 2 cut(s) 160, 798
EcoICRI GAGCTC 2 cut(s) 160, 798
EcoRI GAATTC 1 cut(s) 281
EcoRII CCWGG 1 cut(s) 420
EcoT38I GRGCYC 2 cut(s) 162, 800
FauI CCCGC 2 cut(s) 132, 144
FauNDI CATATG 1 cut(s) 1061
Fnu4HI GCNGC 3 cut(s) 88, 97, 118
FokI GGATG 4 cut(s) 848, 881, 911, 927
FriOI GRGCYC 2 cut(s) 162, 800
Fsp4HI GCNGC 3 cut(s) 88, 97, 118
FspBI CTAG 2 cut(s) 84, 1279
GluI GCNGC 3 cut(s) 88, 97, 118
HaeIII GGCC 8 cut(s) 150, 243, 269, 343, 605, 1113, 1123, 1188
HindIII AAGCTT 2 cut(s) 774, 1085
HinfI GANTC 3 cut(s) 143, 646, 686
HphI GGTGA 6 cut(s) 340, 377, 382, 410, 944, 1137
Hpy166II GTNNAC 1 cut(s) 194
Hpy188I TCNGA 5 cut(s) 463, 552, 670, 865, 898
Hpy188III TCNNGA 3 cut(s) 523, 630, 701
Hpy8I GTNNAC 1 cut(s) 194
HpyAV CCTTC 3 cut(s) 439, 616, 805
HpyCH4III ACNGT 1 cut(s) 394
HpyCH4V TGCA 4 cut(s) 681, 758, 1032, 1138
HpyF10VI GCNNNNNNNGC 6 cut(s) 84, 93, 96, 102, 117, 249
HpyF3I CTNAG 5 cut(s) 67, 253, 290, 864, 1039
KspI CCGCGG 1 cut(s) 140
Kzo9I GATC 8 cut(s) 166, 221, 458, 573, 632, 663, 855, 1101
LguI GCTCTTC 1 cut(s) 788
LmnI GCTCC 2 cut(s) 23, 165
Lsp1109I GCAGC 3 cut(s) 99, 108, 129
LweI GCATC 2 cut(s) 697, 1151
MaeI CTAG 2 cut(s) 84, 1279
MaeIII GTNAC 1 cut(s) 388
MalI GATC 8 cut(s) 168, 223, 460, 575, 634, 665, 857, 1103
MboI GATC 8 cut(s) 166, 221, 458, 573, 632, 663, 855, 1101
MboII GAAGA 2 cut(s) 805, 1078
MflI RGATCY 1 cut(s) 663
MhlI GDGCHC 3 cut(s) 162, 237, 800
MlsI TGGCCA 2 cut(s) 243, 1113
MluCI AATT 7 cut(s) 48, 281, 535, 546, 768, 847, 948
MluNI TGGCCA 2 cut(s) 243, 1113
MlyI GAGTC 1 cut(s) 640
Mox20I TGGCCA 2 cut(s) 243, 1113
MroXI GAANNNNTTC 1 cut(s) 285
MscI TGGCCA 2 cut(s) 243, 1113
MseI TTAA 6 cut(s) 651, 714, 771, 846, 1128, 1202
MslI CAYNNNNRTG 3 cut(s) 317, 705, 834
Msp20I TGGCCA 2 cut(s) 243, 1113
MspA1I CMGCKG 2 cut(s) 139, 1043
MspCI CTTAAG 2 cut(s) 650, 1201
MspR9I CCNGG 1 cut(s) 422
MvaI CCWGG 1 cut(s) 422
MvnI CGCG 1 cut(s) 139
MwoI GCNNNNNNNGC 6 cut(s) 84, 93, 96, 102, 117, 249
NdeI CATATG 1 cut(s) 1061
NdeII GATC 8 cut(s) 166, 221, 458, 573, 632, 663, 855, 1101
NlaIV GGNNCC 2 cut(s) 1122, 1218
NmuCI GTSAC 1 cut(s) 388
NspI RCATGY 1 cut(s) 732
PagI TCATGA 2 cut(s) 522, 700
PceI AGGCCT 2 cut(s) 343, 1188
PciSI GCTCTTC 1 cut(s) 788
PcsI WCGNNNNNNNCGW 1 cut(s) 582
PdmI GAANNNNTTC 1 cut(s) 285
PfeI GAWTC 2 cut(s) 143, 686
PkrI GCNGC 3 cut(s) 89, 98, 119
Ple19I CGATCG 1 cut(s) 461
PleI GAGTC 1 cut(s) 640
PpsI GAGTC 1 cut(s) 640
PshBI ATTAAT 1 cut(s) 714
Psp124BI GAGCTC 2 cut(s) 162, 800
Psp6I CCWGG 1 cut(s) 420
PspGI CCWGG 1 cut(s) 420
PspN4I GGNNCC 2 cut(s) 1122, 1218
PspPI GGNCC 1 cut(s) 1121
PstI CTGCAG 1 cut(s) 760
PsuI RGATCY 1 cut(s) 663
PvuI CGATCG 1 cut(s) 461
PvuII CAGCTG 1 cut(s) 1043
RsaI GTAC 1 cut(s) 195
RsaNI GTAC 1 cut(s) 194
RseI CAYNNNNRTG 3 cut(s) 317, 705, 834
SacI GAGCTC 2 cut(s) 162, 800
SacII CCGCGG 1 cut(s) 140
SapI GCTCTTC 1 cut(s) 788
SaqAI TTAA 6 cut(s) 651, 714, 771, 846, 1128, 1202
SatI GCNGC 3 cut(s) 88, 97, 118
Sau3AI GATC 8 cut(s) 166, 221, 458, 573, 632, 663, 855, 1101
Sau96I GGNCC 1 cut(s) 1121
SchI GAGTC 1 cut(s) 640
ScrFI CCNGG 1 cut(s) 422
SduI GDGCHC 3 cut(s) 162, 237, 800
SfaNI GCATC 2 cut(s) 697, 1151
SfcI CTRYAG 1 cut(s) 756
Sfr303I CCGCGG 1 cut(s) 140
SgrBI CCGCGG 1 cut(s) 140
SmiMI CAYNNNNRTG 3 cut(s) 317, 705, 834
SmlI CTYRAG 2 cut(s) 650, 1201
SmoI CTYRAG 2 cut(s) 650, 1201
Sse9I AATT 7 cut(s) 48, 281, 535, 546, 768, 847, 948
SseBI AGGCCT 2 cut(s) 343, 1188
SsiI CCGC 3 cut(s) 137, 139, 230
SspMI CTAG 2 cut(s) 84, 1279
SstI GAGCTC 2 cut(s) 162, 800
StuI AGGCCT 2 cut(s) 343, 1188
StyD4I CCNGG 1 cut(s) 420
TaaI ACNGT 1 cut(s) 394
TaqI TCGA 3 cut(s) 156, 220, 576
TasI AATT 7 cut(s) 48, 281, 535, 546, 768, 847, 948
TfiI GAWTC 2 cut(s) 143, 686
Tru1I TTAA 6 cut(s) 651, 714, 771, 846, 1128, 1202
Tru9I TTAA 6 cut(s) 651, 714, 771, 846, 1128, 1202
TscAI CASTG 2 cut(s) 298, 1039
TseFI GTSAC 1 cut(s) 388
TseI GCWGC 3 cut(s) 87, 96, 117
Tsp45I GTSAC 1 cut(s) 388
TspDTI ATGAA 6 cut(s) 144, 249, 274, 511, 539, 1159
TspRI CASTG 2 cut(s) 298, 1039
Vha464I CTTAAG 2 cut(s) 650, 1201
VspI ATTAAT 1 cut(s) 714
XapI RAATTY 2 cut(s) 281, 546
XceI RCATGY 1 cut(s) 732
XmnI GAANNNNTTC 1 cut(s) 285
XspI CTAG 2 cut(s) 84, 1279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.