FvH4_7g06311

Belongs to the pyrroline-5-carboxylate reductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0018872
Physical Location & Seq
Reverse (-)
6399967 .. 6400958
992 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g06311.t1

Sequence Viewer

Length: 249 bp
ATGGAGGAGTTGCTGCAGGTCTACCGCGAGCTAGAACTTGCATTGGGTCTAGCATCTCAAACTGTATTAGGAGCAGCATCTATGGCTACTAGTTCTAGGAAACATCCAGGTCAGCTAAAAGATGATGTAACATCACCTGGTGGAACCATTACTGGCATTCATGAGCTGGAGAAGGGTGGATTTCGTGGAATATTGATGAATGCAGTCGTTGCTGCTGCCAGGCGCAGCCGAGAACTTTCACAGAACTAG

Protein Analysis

83

Amino Acids

8.73

Weight (kDa)

6.72

Isoelectric Point (pI)

42.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
P5CR_dimer PF14748 12 - 79 3.3e-20 Pyrroline-5-carboxylate reductase dimerisation
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018872)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g06311
rosa_chinensis RchiOBHm_Chr2g0125021
rosa_multiflora Rmu_sc0001251.1_g000011 Rmu_sc0018900.1_g000003
rosa_roxburghii Rroxscaffold_2G00118400 Rroxscaffold_2G00118410 Rroxscaffold_2G00118430 Rroxscaffold_2G00118750
rosa_samantha Rh2DG340200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 7
AccI GTMKAC 1 cut(s) 21
AccII CGCG 1 cut(s) 27
AciI CCGC 1 cut(s) 25
AdeI CACNNNGTG 1 cut(s) 140
AhlI ACTAGT 1 cut(s) 89
AjnI CCWGG 3 cut(s) 106, 136, 218
AluBI AGCT 3 cut(s) 31, 115, 166
AluI AGCT 3 cut(s) 31, 115, 166
ApeKI GCWGC 5 cut(s) 13, 74, 212, 215, 225
AspLEI GCGC 1 cut(s) 225
AsuHPI GGTGA 1 cut(s) 126
BbvI GCAGC 4 cut(s) 86, 199, 202, 237
BciT130I CCWGG 3 cut(s) 108, 138, 220
BcuI ACTAGT 1 cut(s) 89
BfaI CTAG 5 cut(s) 32, 50, 90, 96, 247
BfmI CTRYAG 1 cut(s) 14
BfuAI ACCTGC 1 cut(s) 7
BisI GCNGC 5 cut(s) 14, 75, 213, 216, 226
BlsI GCNGC 5 cut(s) 15, 76, 214, 217, 227
Bme1390I CCNGG 3 cut(s) 108, 138, 220
BmiI GGNNCC 1 cut(s) 145
BmrFI CCNGG 3 cut(s) 108, 138, 220
BmsI GCATC 2 cut(s) 62, 86
BpmI CTGGAG 1 cut(s) 188
Bse1I ACTGG 1 cut(s) 157
BseBI CCWGG 3 cut(s) 108, 138, 220
BseGI GGATG 1 cut(s) 103
BseNI ACTGG 1 cut(s) 157
BseRI GAGGAG 1 cut(s) 20
BseXI GCAGC 4 cut(s) 86, 199, 202, 237
Bsh1236I CGCG 1 cut(s) 27
BsmI GAATGC 2 cut(s) 156, 205
BspACI CCGC 1 cut(s) 25
BspFNI CGCG 1 cut(s) 27
BspHI TCATGA 1 cut(s) 160
BspLI GGNNCC 1 cut(s) 145
BspMAI CTGCAG 1 cut(s) 18
BspMI ACCTGC 1 cut(s) 7
BsrI ACTGG 1 cut(s) 157
Bst2UI CCWGG 3 cut(s) 108, 138, 220
Bst4CI ACNGT 1 cut(s) 64
BstAPI GCANNNNNTGC 1 cut(s) 209
BstC8I GCNNGC 1 cut(s) 29
BstF5I GGATG 1 cut(s) 103
BstFNI CGCG 1 cut(s) 27
BstHHI GCGC 1 cut(s) 225
BstMWI GCNNNNNNNGC 2 cut(s) 83, 209
BstNI CCWGG 3 cut(s) 108, 138, 220
BstSCI CCNGG 3 cut(s) 106, 136, 218
BstSFI CTRYAG 1 cut(s) 14
BstUI CGCG 1 cut(s) 27
BstV1I GCAGC 4 cut(s) 86, 199, 202, 237
BtsCI GGATG 1 cut(s) 103
BveI ACCTGC 1 cut(s) 7
Cac8I GCNNGC 1 cut(s) 29
CciI TCATGA 1 cut(s) 160
CfoI GCGC 1 cut(s) 225
CsiI ACCWGGT 1 cut(s) 136
CviAII CATG 1 cut(s) 161
CviJI RGCY 5 cut(s) 31, 86, 115, 166, 228
CviKI_1 RGCY 5 cut(s) 31, 86, 115, 166, 228
DraIII CACNNNGTG 1 cut(s) 140
EcoRII CCWGG 3 cut(s) 106, 136, 218
FaeI CATG 1 cut(s) 164
FaiI YATR 2 cut(s) 83, 162
FatI CATG 1 cut(s) 160
FblI GTMKAC 1 cut(s) 21
Fnu4HI GCNGC 5 cut(s) 14, 75, 213, 216, 226
FokI GGATG 1 cut(s) 90
Fsp4HI GCNGC 5 cut(s) 14, 75, 213, 216, 226
FspBI CTAG 5 cut(s) 32, 50, 90, 96, 247
GlaI GCGC 1 cut(s) 224
GluI GCNGC 5 cut(s) 14, 75, 213, 216, 226
GsuI CTGGAG 1 cut(s) 188
HhaI GCGC 1 cut(s) 225
Hin1II CATG 1 cut(s) 164
Hin6I GCGC 1 cut(s) 223
HinP1I GCGC 1 cut(s) 223
HphI GGTGA 1 cut(s) 126
Hpy166II GTNNAC 1 cut(s) 22
Hpy188III TCNNGA 1 cut(s) 161
Hpy8I GTNNAC 1 cut(s) 22
HpyAV CCTTC 1 cut(s) 166
HpyCH4III ACNGT 1 cut(s) 64
HpyCH4V TGCA 3 cut(s) 16, 41, 203
HpyF10VI GCNNNNNNNGC 2 cut(s) 83, 209
Hsp92II CATG 1 cut(s) 164
HspAI GCGC 1 cut(s) 223
LmnI GCTCC 1 cut(s) 71
LpnPI CCDG 9 cut(s) 2, 93, 120, 123, 138, 150, 152, 205, 232
Lsp1109I GCAGC 4 cut(s) 86, 199, 202, 237
LweI GCATC 2 cut(s) 62, 86
MabI ACCWGGT 1 cut(s) 136
MaeI CTAG 5 cut(s) 32, 50, 90, 96, 247
MaeIII GTNAC 1 cut(s) 127
MspR9I CCNGG 3 cut(s) 108, 138, 220
Mva1269I GAATGC 2 cut(s) 156, 205
MvaI CCWGG 3 cut(s) 108, 138, 220
MvnI CGCG 1 cut(s) 27
MwoI GCNNNNNNNGC 2 cut(s) 83, 209
NlaIII CATG 1 cut(s) 164
NlaIV GGNNCC 1 cut(s) 145
PagI TCATGA 1 cut(s) 160
PctI GAATGC 2 cut(s) 156, 205
PkrI GCNGC 5 cut(s) 15, 76, 214, 217, 227
Psp6I CCWGG 3 cut(s) 106, 136, 218
PspGI CCWGG 3 cut(s) 106, 136, 218
PspN4I GGNNCC 1 cut(s) 145
PstI CTGCAG 1 cut(s) 18
SatI GCNGC 5 cut(s) 14, 75, 213, 216, 226
ScrFI CCNGG 3 cut(s) 108, 138, 220
SetI ASST 6 cut(s) 21, 33, 112, 117, 139, 168
SexAI ACCWGGT 1 cut(s) 136
SfaNI GCATC 2 cut(s) 62, 86
SfcI CTRYAG 1 cut(s) 14
SpeI ACTAGT 1 cut(s) 89
SsiI CCGC 1 cut(s) 25
SspI AATATT 1 cut(s) 192
SspMI CTAG 5 cut(s) 32, 50, 90, 96, 247
StyD4I CCNGG 3 cut(s) 106, 136, 218
TaaI ACNGT 1 cut(s) 64
TseI GCWGC 5 cut(s) 13, 74, 212, 215, 225
TspDTI ATGAA 2 cut(s) 149, 212
XmiI GTMKAC 1 cut(s) 21
XspI CTAG 5 cut(s) 32, 50, 90, 96, 247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.