Rroxscaffold_2G00118410

Belongs to the pyrroline-5-carboxylate reductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0018872
Physical Location & Seq
Forward (+)
49880042 .. 49888709
8668 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00118410.1

Sequence Viewer

Length: 279 bp
ATGGCCGGAAACTGGGGAATTTTGCCGGGGAAGCAAACTGCTACAGTATTAGGAGCAGCATCTATGGCTACTAGTTCTGGGAAGCATCCAGGTCAGCTAAAAGATGATGTAACATCACCTGGTGGAACTACCATTACCGGCATTCATGAGCTGGAGAAGGGTGGATTTGGTGGAATATTGATGAATGCGATCGTTGCTGCTGCTGGCGCAGCCGAGAACTTTCACAGAACTAGTACCTTGAAAATGGCACCAAATGAAGACATGGCTTTCCATGAATGA

Protein Analysis

92

Amino Acids

9.35

Weight (kDa)

6.03

Isoelectric Point (pI)

26.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
P5CR_dimer PF14748 14 - 72 1.2e-17 Pyrroline-5-carboxylate reductase dimerisation
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018872)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g06311
rosa_chinensis RchiOBHm_Chr2g0125021
rosa_multiflora Rmu_sc0001251.1_g000011 Rmu_sc0018900.1_g000003
rosa_roxburghii Rroxscaffold_2G00118400 Rroxscaffold_2G00118410 Rroxscaffold_2G00118430 Rroxscaffold_2G00118750
rosa_samantha Rh2DG340200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 247
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 18
AdeI CACNNNGTG 1 cut(s) 122
AfaI GTAC 1 cut(s) 235
AfiI CCNNNNNNNGG 1 cut(s) 12
AgsI TTSAA 1 cut(s) 241
AhlI ACTAGT 2 cut(s) 71, 230
AjnI CCWGG 2 cut(s) 88, 118
AluBI AGCT 2 cut(s) 97, 151
AluI AGCT 2 cut(s) 97, 151
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 4 cut(s) 56, 197, 200, 209
ApoI RAATTY 1 cut(s) 18
AspLEI GCGC 1 cut(s) 209
AsuC2I CCSGG 1 cut(s) 27
AsuHPI GGTGA 1 cut(s) 108
BanI GGYRCC 1 cut(s) 247
BbsI GAAGAC 1 cut(s) 264
BbvI GCAGC 4 cut(s) 68, 184, 187, 221
BciT130I CCWGG 2 cut(s) 90, 120
BcnI CCSGG 1 cut(s) 27
BcuI ACTAGT 2 cut(s) 71, 230
BfaI CTAG 2 cut(s) 72, 231
BfmI CTRYAG 1 cut(s) 42
BisI GCNGC 4 cut(s) 57, 198, 201, 210
BlsI GCNGC 4 cut(s) 58, 199, 202, 211
Bme1390I CCNGG 3 cut(s) 27, 90, 120
BmiI GGNNCC 1 cut(s) 249
BmrFI CCNGG 3 cut(s) 27, 90, 120
BmrI ACTGGG 1 cut(s) 22
BmsI GCATC 2 cut(s) 68, 94
BmuI ACTGGG 1 cut(s) 22
BpiI GAAGAC 1 cut(s) 264
BpmI CTGGAG 1 cut(s) 173
BpuMI CCSGG 1 cut(s) 27
BsaJI CCNNGG 1 cut(s) 26
Bsc4I CCNNNNNNNGG 1 cut(s) 12
Bse118I RCCGGY 1 cut(s) 137
Bse1I ACTGG 1 cut(s) 17
BseBI CCWGG 2 cut(s) 90, 120
BseDI CCNNGG 1 cut(s) 26
BseGI GGATG 1 cut(s) 85
BseLI CCNNNNNNNGG 1 cut(s) 12
BseNI ACTGG 1 cut(s) 17
BseXI GCAGC 4 cut(s) 68, 184, 187, 221
Bsh1285I CGRYCG 1 cut(s) 192
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 247
BsiEI CGRYCG 1 cut(s) 192
BsiSI CCGG 3 cut(s) 6, 26, 138
BslI CCNNNNNNNGG 1 cut(s) 12
BsmI GAATGC 2 cut(s) 141, 190
BsnI GGCC 1 cut(s) 5
Bsp143I GATC 1 cut(s) 189
BspANI GGCC 1 cut(s) 5
BspHI TCATGA 1 cut(s) 145
BspLI GGNNCC 1 cut(s) 249
BspT107I GGYRCC 1 cut(s) 247
BsrFI RCCGGY 1 cut(s) 137
BsrI ACTGG 1 cut(s) 17
BssAI RCCGGY 1 cut(s) 137
BssECI CCNNGG 1 cut(s) 26
BssMI GATC 1 cut(s) 189
Bst2UI CCWGG 2 cut(s) 90, 120
Bst4CI ACNGT 1 cut(s) 46
BstC8I GCNNGC 1 cut(s) 205
BstF5I GGATG 1 cut(s) 85
BstHHI GCGC 1 cut(s) 209
BstKTI GATC 1 cut(s) 192
BstMBI GATC 1 cut(s) 189
BstMCI CGRYCG 1 cut(s) 192
BstMWI GCNNNNNNNGC 5 cut(s) 31, 65, 194, 206, 209
BstNI CCWGG 2 cut(s) 90, 120
BstSCI CCNGG 3 cut(s) 25, 88, 118
BstSFI CTRYAG 1 cut(s) 42
BstV1I GCAGC 4 cut(s) 68, 184, 187, 221
BstV2I GAAGAC 1 cut(s) 264
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 1 cut(s) 85
Cac8I GCNNGC 1 cut(s) 205
CciI TCATGA 1 cut(s) 145
CfoI GCGC 1 cut(s) 209
Cfr10I RCCGGY 1 cut(s) 137
CsiI ACCWGGT 1 cut(s) 118
Csp6I GTAC 1 cut(s) 234
CviAII CATG 3 cut(s) 146, 262, 272
CviJI RGCY 6 cut(s) 5, 68, 97, 151, 212, 266
CviKI_1 RGCY 6 cut(s) 5, 68, 97, 151, 212, 266
CviQI GTAC 1 cut(s) 234
DpnI GATC 1 cut(s) 191
DpnII GATC 1 cut(s) 189
DraIII CACNNNGTG 1 cut(s) 122
EaeI YGGCCR 1 cut(s) 3
EcoRII CCWGG 2 cut(s) 88, 118
FaeI CATG 3 cut(s) 149, 265, 275
FaiI YATR 4 cut(s) 65, 147, 263, 273
FatI CATG 3 cut(s) 145, 261, 271
Fnu4HI GCNGC 4 cut(s) 57, 198, 201, 210
FokI GGATG 1 cut(s) 72
Fsp4HI GCNGC 4 cut(s) 57, 198, 201, 210
FspBI CTAG 2 cut(s) 72, 231
GlaI GCGC 1 cut(s) 208
GluI GCNGC 4 cut(s) 57, 198, 201, 210
GsuI CTGGAG 1 cut(s) 173
HaeIII GGCC 1 cut(s) 5
HapII CCGG 3 cut(s) 6, 26, 138
HhaI GCGC 1 cut(s) 209
Hin1II CATG 3 cut(s) 149, 265, 275
Hin6I GCGC 1 cut(s) 207
HinP1I GCGC 1 cut(s) 207
HpaII CCGG 3 cut(s) 6, 26, 138
HphI GGTGA 1 cut(s) 108
Hpy188III TCNNGA 1 cut(s) 146
HpyAV CCTTC 1 cut(s) 151
HpyCH4III ACNGT 1 cut(s) 46
HpyF10VI GCNNNNNNNGC 5 cut(s) 31, 65, 194, 206, 209
Hsp92II CATG 3 cut(s) 149, 265, 275
HspAI GCGC 1 cut(s) 207
Kzo9I GATC 1 cut(s) 189
LmnI GCTCC 1 cut(s) 53
Lsp1109I GCAGC 4 cut(s) 68, 184, 187, 221
LweI GCATC 2 cut(s) 68, 94
MabI ACCWGGT 1 cut(s) 118
MaeI CTAG 2 cut(s) 72, 231
MaeIII GTNAC 1 cut(s) 109
MalI GATC 1 cut(s) 191
MboI GATC 1 cut(s) 189
MboII GAAGA 1 cut(s) 269
MluCI AATT 1 cut(s) 18
MspI CCGG 3 cut(s) 6, 26, 138
MspR9I CCNGG 3 cut(s) 27, 90, 120
Mva1269I GAATGC 2 cut(s) 141, 190
MvaI CCWGG 2 cut(s) 90, 120
MwoI GCNNNNNNNGC 5 cut(s) 31, 65, 194, 206, 209
NciI CCSGG 1 cut(s) 27
NdeII GATC 1 cut(s) 189
NlaIII CATG 3 cut(s) 149, 265, 275
NlaIV GGNNCC 1 cut(s) 249
NmeAIII GCCGAG 1 cut(s) 238
PagI TCATGA 1 cut(s) 145
PctI GAATGC 2 cut(s) 141, 190
PkrI GCNGC 4 cut(s) 58, 199, 202, 211
Ple19I CGATCG 1 cut(s) 192
Psp6I CCWGG 2 cut(s) 88, 118
PspGI CCWGG 2 cut(s) 88, 118
PspN4I GGNNCC 1 cut(s) 249
PsrI GAACNNNNNNTAC 2 cut(s) 118, 150
PvuI CGATCG 1 cut(s) 192
RsaI GTAC 1 cut(s) 235
RsaNI GTAC 1 cut(s) 234
SatI GCNGC 4 cut(s) 57, 198, 201, 210
Sau3AI GATC 1 cut(s) 189
ScrFI CCNGG 3 cut(s) 27, 90, 120
SetI ASST 5 cut(s) 94, 99, 121, 153, 239
SexAI ACCWGGT 1 cut(s) 118
SfaNI GCATC 2 cut(s) 68, 94
SfcI CTRYAG 1 cut(s) 42
SpeI ACTAGT 2 cut(s) 71, 230
Sse9I AATT 1 cut(s) 18
SspI AATATT 1 cut(s) 177
SspMI CTAG 2 cut(s) 72, 231
StyD4I CCNGG 3 cut(s) 25, 88, 118
TaaI ACNGT 1 cut(s) 46
TasI AATT 1 cut(s) 18
TseI GCWGC 4 cut(s) 56, 197, 200, 209
TspDTI ATGAA 3 cut(s) 134, 197, 270
XapI RAATTY 1 cut(s) 18
XspI CTAG 2 cut(s) 72, 231
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.