Rroxscaffold_2G00118430

Belongs to the pyrroline-5-carboxylate reductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0018872
Physical Location & Seq
Forward (+)
49966184 .. 49973070
6887 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00118430.1

Sequence Viewer

Length: 327 bp
ATGAGAGAGACATCGGCCAGTGAAGAAAGAGCAGCTCGGGAGAATTCCAGCTCGAGCTATGTAGCTTCTCGGCCTCCTAGCGCCTTCCACGGGGCATTACTCGTTATGCAATTTCCATTTATTCTTCTTGGATTCCTTGTATTAGGAGCAGCATCTATGGCTACTAGTTCTGGGAAGCATCCAGGTCAGCTAAAAGATGATGTAACATCACCTGGTGGAACTACCATTACTGGCATTCATGAGCTGGAGAAGGGTGGATTTCGTGGAATATTGATGAATGCAGTTGTTGCTGCTGCCAGGCGCAGCCGAGAACTTTCACAGAACTAG

Protein Analysis

108

Amino Acids

11.39

Weight (kDa)

9.51

Isoelectric Point (pI)

45.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
P5CR_dimer PF14748 46 - 105 9.2e-20 Pyrroline-5-carboxylate reductase dimerisation
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018872)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g06311
rosa_chinensis RchiOBHm_Chr2g0125021
rosa_multiflora Rmu_sc0001251.1_g000011 Rmu_sc0018900.1_g000003
rosa_roxburghii Rroxscaffold_2G00118400 Rroxscaffold_2G00118410 Rroxscaffold_2G00118430 Rroxscaffold_2G00118750
rosa_samantha Rh2DG340200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 15
AcsI RAATTY 1 cut(s) 43
AdeI CACNNNGTG 1 cut(s) 215
AfiI CCNNNNNNNGG 1 cut(s) 90
AhlI ACTAGT 1 cut(s) 164
AjnI CCWGG 3 cut(s) 181, 211, 296
AluBI AGCT 6 cut(s) 35, 51, 57, 65, 190, 244
AluI AGCT 6 cut(s) 35, 51, 57, 65, 190, 244
Alw26I GTCTC 1 cut(s) 2
Ama87I CYCGRG 2 cut(s) 36, 52
AoxI GGCC 2 cut(s) 15, 71
ApeKI GCWGC 5 cut(s) 32, 149, 290, 293, 303
ApoI RAATTY 1 cut(s) 43
AspLEI GCGC 2 cut(s) 83, 303
AsuHPI GGTGA 1 cut(s) 201
AvaI CYCGRG 2 cut(s) 36, 52
BbvI GCAGC 5 cut(s) 44, 161, 277, 280, 315
BciT130I CCWGG 3 cut(s) 183, 213, 298
BcoDI GTCTC 1 cut(s) 2
BcuI ACTAGT 1 cut(s) 164
BfaI CTAG 3 cut(s) 78, 165, 325
BfoI RGCGCY 1 cut(s) 84
BisI GCNGC 5 cut(s) 33, 150, 291, 294, 304
BlsI GCNGC 5 cut(s) 34, 151, 292, 295, 305
Bme1390I CCNGG 3 cut(s) 183, 213, 298
BmeT110I CYCGRG 2 cut(s) 36, 52
BmrFI CCNGG 3 cut(s) 183, 213, 298
BmsI GCATC 2 cut(s) 161, 187
BpmI CTGGAG 1 cut(s) 266
BsaJI CCNNGG 1 cut(s) 88
Bsc4I CCNNNNNNNGG 1 cut(s) 90
Bse1I ACTGG 2 cut(s) 18, 235
BseBI CCWGG 3 cut(s) 183, 213, 298
BseDI CCNNGG 1 cut(s) 88
BseGI GGATG 1 cut(s) 178
BseLI CCNNNNNNNGG 1 cut(s) 90
BseNI ACTGG 2 cut(s) 18, 235
BseXI GCAGC 5 cut(s) 44, 161, 277, 280, 315
BshFI GGCC 2 cut(s) 17, 73
BsiHKCI CYCGRG 2 cut(s) 36, 52
BslI CCNNNNNNNGG 1 cut(s) 90
BsmAI GTCTC 1 cut(s) 2
BsmI GAATGC 2 cut(s) 234, 283
BsnI GGCC 2 cut(s) 17, 73
BsoBI CYCGRG 2 cut(s) 36, 52
BspANI GGCC 2 cut(s) 17, 73
BspHI TCATGA 1 cut(s) 238
BsrI ACTGG 2 cut(s) 18, 235
BssECI CCNNGG 1 cut(s) 88
Bst2UI CCWGG 3 cut(s) 183, 213, 298
BstAPI GCANNNNNTGC 1 cut(s) 287
BstDSI CCRYGG 1 cut(s) 88
BstF5I GGATG 1 cut(s) 178
BstH2I RGCGCY 1 cut(s) 84
BstHHI GCGC 2 cut(s) 83, 303
BstMAI GTCTC 1 cut(s) 2
BstMWI GCNNNNNNNGC 2 cut(s) 158, 287
BstNI CCWGG 3 cut(s) 183, 213, 298
BstSCI CCNGG 3 cut(s) 181, 211, 296
BstV1I GCAGC 5 cut(s) 44, 161, 277, 280, 315
BsuRI GGCC 2 cut(s) 17, 73
BtgI CCRYGG 1 cut(s) 88
BtsCI GGATG 1 cut(s) 178
BtsIMutI CAGTG 1 cut(s) 25
CciI TCATGA 1 cut(s) 238
CfoI GCGC 2 cut(s) 83, 303
CsiI ACCWGGT 1 cut(s) 211
CviAII CATG 1 cut(s) 239
DraIII CACNNNGTG 1 cut(s) 215
EaeI YGGCCR 1 cut(s) 15
Eco88I CYCGRG 2 cut(s) 36, 52
EcoRI GAATTC 1 cut(s) 43
EcoRII CCWGG 3 cut(s) 181, 211, 296
FaeI CATG 1 cut(s) 242
FaiI YATR 4 cut(s) 60, 107, 158, 240
FatI CATG 1 cut(s) 238
Fnu4HI GCNGC 5 cut(s) 33, 150, 291, 294, 304
FokI GGATG 1 cut(s) 165
Fsp4HI GCNGC 5 cut(s) 33, 150, 291, 294, 304
FspBI CTAG 3 cut(s) 78, 165, 325
GlaI GCGC 2 cut(s) 82, 302
GluI GCNGC 5 cut(s) 33, 150, 291, 294, 304
GsuI CTGGAG 1 cut(s) 266
HaeII RGCGCY 1 cut(s) 84
HaeIII GGCC 2 cut(s) 17, 73
HhaI GCGC 2 cut(s) 83, 303
Hin1II CATG 1 cut(s) 242
Hin6I GCGC 2 cut(s) 81, 301
HinP1I GCGC 2 cut(s) 81, 301
HinfI GANTC 1 cut(s) 132
HphI GGTGA 1 cut(s) 201
Hpy188III TCNNGA 2 cut(s) 38, 239
HpyAV CCTTC 2 cut(s) 94, 244
HpyCH4V TGCA 2 cut(s) 109, 281
HpyF10VI GCNNNNNNNGC 2 cut(s) 158, 287
Hsp92II CATG 1 cut(s) 242
HspAI GCGC 2 cut(s) 81, 301
LmnI GCTCC 1 cut(s) 146
Lsp1109I GCAGC 5 cut(s) 44, 161, 277, 280, 315
LweI GCATC 2 cut(s) 161, 187
MabI ACCWGGT 1 cut(s) 211
MaeI CTAG 3 cut(s) 78, 165, 325
MaeIII GTNAC 1 cut(s) 202
MboII GAAGA 2 cut(s) 35, 116
MluCI AATT 2 cut(s) 43, 110
MnlI CCTC 1 cut(s) 84
MspR9I CCNGG 3 cut(s) 183, 213, 298
Mva1269I GAATGC 2 cut(s) 234, 283
MvaI CCWGG 3 cut(s) 183, 213, 298
MwoI GCNNNNNNNGC 2 cut(s) 158, 287
NlaIII CATG 1 cut(s) 242
NmeAIII GCCGAG 1 cut(s) 49
PaeR7I CTCGAG 1 cut(s) 52
PagI TCATGA 1 cut(s) 238
PctI GAATGC 2 cut(s) 234, 283
PfeI GAWTC 1 cut(s) 132
PkrI GCNGC 5 cut(s) 34, 151, 292, 295, 305
Psp6I CCWGG 3 cut(s) 181, 211, 296
PspGI CCWGG 3 cut(s) 181, 211, 296
PspXI VCTCGAGB 1 cut(s) 52
PsrI GAACNNNNNNTAC 2 cut(s) 211, 243
SatI GCNGC 5 cut(s) 33, 150, 291, 294, 304
ScrFI CCNGG 3 cut(s) 183, 213, 298
SetI ASST 8 cut(s) 37, 53, 59, 67, 187, 192, 214, 246
SexAI ACCWGGT 1 cut(s) 211
SfaNI GCATC 2 cut(s) 161, 187
Sfr274I CTCGAG 1 cut(s) 52
SlaI CTCGAG 1 cut(s) 52
SmlI CTYRAG 1 cut(s) 52
SmoI CTYRAG 1 cut(s) 52
SpeI ACTAGT 1 cut(s) 164
Sse9I AATT 2 cut(s) 43, 110
SspI AATATT 1 cut(s) 270
SspMI CTAG 3 cut(s) 78, 165, 325
StyD4I CCNGG 3 cut(s) 181, 211, 296
TaqI TCGA 1 cut(s) 53
TasI AATT 2 cut(s) 43, 110
TfiI GAWTC 1 cut(s) 132
TscAI CASTG 1 cut(s) 25
TseI GCWGC 5 cut(s) 32, 149, 290, 293, 303
TspDTI ATGAA 2 cut(s) 227, 290
TspRI CASTG 1 cut(s) 25
XapI RAATTY 1 cut(s) 43
XhoI CTCGAG 1 cut(s) 52
XspI CTAG 3 cut(s) 78, 165, 325
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.