Rh2DG340200

Belongs to the pyrroline-5-carboxylate reductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0018872
Physical Location & Seq
Reverse (-)
43999613 .. 44003865
4253 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG340200.1

Sequence Viewer

Length: 333 bp
ATGGTTATTACGCAACATCATTGTGATGTAATGAAGATTCTCTTTGCTCTTGCCTTAATGGGGAAGGCCCCCAAACAAGCAAATGCTCTCTCTAAGTTGTTTATGCACGCCAAAACAAAATGTTGGGTCTCTGGCATTTGCAAGGTATTAGGAGCAGCATCTATGGCTACTAGTTCTGGGAAGCATCCAGGTCAGCTTAAAGATGATGTAACATCACCTGGTGGAACTACCATTACTGGCATTCATGAGCTGGAGAAGGGTGGATTTCGTGGAATATTGATGAATGCAGTCGTTGCTGCTGCCAGGCGCAGCCGAGAACTTTCACAGAACTAG

Protein Analysis

110

Amino Acids

11.67

Weight (kDa)

9.88

Isoelectric Point (pI)

36.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
P5CR_dimer PF14748 49 - 107 1.3e-19 Pyrroline-5-carboxylate reductase dimerisation
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018872)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g06311
rosa_chinensis RchiOBHm_Chr2g0125021
rosa_multiflora Rmu_sc0001251.1_g000011 Rmu_sc0018900.1_g000003
rosa_roxburghii Rroxscaffold_2G00118400 Rroxscaffold_2G00118410 Rroxscaffold_2G00118430 Rroxscaffold_2G00118750
rosa_samantha Rh2DG340200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AdeI CACNNNGTG 1 cut(s) 221
AfiI CCNNNNNNNGG 1 cut(s) 60
AhlI ACTAGT 1 cut(s) 170
AjnI CCWGG 3 cut(s) 187, 217, 302
AluBI AGCT 2 cut(s) 196, 250
AluI AGCT 2 cut(s) 196, 250
Alw26I GTCTC 1 cut(s) 133
AoxI GGCC 1 cut(s) 66
ApeKI GCWGC 4 cut(s) 155, 296, 299, 309
AspLEI GCGC 1 cut(s) 309
AspS9I GGNCC 1 cut(s) 67
AsuHPI GGTGA 1 cut(s) 207
BbvI GCAGC 4 cut(s) 167, 283, 286, 321
BciT130I CCWGG 3 cut(s) 189, 219, 304
BcoDI GTCTC 1 cut(s) 133
BcuI ACTAGT 1 cut(s) 170
BfaI CTAG 2 cut(s) 171, 331
BisI GCNGC 4 cut(s) 156, 297, 300, 310
BlsI GCNGC 4 cut(s) 157, 298, 301, 311
Bme1390I CCNGG 3 cut(s) 189, 219, 304
BmgT120I GGNCC 1 cut(s) 67
BmiI GGNNCC 1 cut(s) 69
BmrFI CCNGG 3 cut(s) 189, 219, 304
BmsI GCATC 2 cut(s) 167, 193
BpmI CTGGAG 1 cut(s) 272
BsaI GGTCTC 1 cut(s) 133
Bsc4I CCNNNNNNNGG 1 cut(s) 60
Bse1I ACTGG 1 cut(s) 241
BseBI CCWGG 3 cut(s) 189, 219, 304
BseGI GGATG 1 cut(s) 184
BseLI CCNNNNNNNGG 1 cut(s) 60
BseNI ACTGG 1 cut(s) 241
BseXI GCAGC 4 cut(s) 167, 283, 286, 321
BshFI GGCC 1 cut(s) 68
BslI CCNNNNNNNGG 1 cut(s) 60
BsmAI GTCTC 1 cut(s) 133
BsmI GAATGC 2 cut(s) 240, 289
BsnI GGCC 1 cut(s) 68
Bso31I GGTCTC 1 cut(s) 133
BspANI GGCC 1 cut(s) 68
BspHI TCATGA 1 cut(s) 244
BspLI GGNNCC 1 cut(s) 69
BspTNI GGTCTC 1 cut(s) 133
BsrI ACTGG 1 cut(s) 241
Bst2UI CCWGG 3 cut(s) 189, 219, 304
BstAPI GCANNNNNTGC 1 cut(s) 293
BstC8I GCNNGC 1 cut(s) 108
BstDEI CTNAG 1 cut(s) 93
BstF5I GGATG 1 cut(s) 184
BstHHI GCGC 1 cut(s) 309
BstMAI GTCTC 1 cut(s) 133
BstMWI GCNNNNNNNGC 2 cut(s) 164, 293
BstNI CCWGG 3 cut(s) 189, 219, 304
BstSCI CCNGG 3 cut(s) 187, 217, 302
BstV1I GCAGC 4 cut(s) 167, 283, 286, 321
BsuRI GGCC 1 cut(s) 68
BtsCI GGATG 1 cut(s) 184
Cac8I GCNNGC 1 cut(s) 108
CciI TCATGA 1 cut(s) 244
CfoI GCGC 1 cut(s) 309
Cfr13I GGNCC 1 cut(s) 67
CsiI ACCWGGT 1 cut(s) 217
CviAII CATG 1 cut(s) 245
CviJI RGCY 5 cut(s) 68, 167, 196, 250, 312
CviKI_1 RGCY 5 cut(s) 68, 167, 196, 250, 312
DdeI CTNAG 1 cut(s) 93
DraIII CACNNNGTG 1 cut(s) 221
Eco31I GGTCTC 1 cut(s) 133
EcoO109I RGGNCCY 1 cut(s) 67
EcoRII CCWGG 3 cut(s) 187, 217, 302
FaeI CATG 1 cut(s) 248
FaiI YATR 3 cut(s) 104, 164, 246
FalI AAGNNNNNCTT 2 cut(s) 26, 58
FatI CATG 1 cut(s) 244
Fnu4HI GCNGC 4 cut(s) 156, 297, 300, 310
FokI GGATG 1 cut(s) 171
Fsp4HI GCNGC 4 cut(s) 156, 297, 300, 310
FspBI CTAG 2 cut(s) 171, 331
GlaI GCGC 1 cut(s) 308
GluI GCNGC 4 cut(s) 156, 297, 300, 310
GsuI CTGGAG 1 cut(s) 272
HaeIII GGCC 1 cut(s) 68
HhaI GCGC 1 cut(s) 309
Hin1II CATG 1 cut(s) 248
Hin6I GCGC 1 cut(s) 307
HinP1I GCGC 1 cut(s) 307
HinfI GANTC 1 cut(s) 37
HphI GGTGA 1 cut(s) 207
Hpy188III TCNNGA 1 cut(s) 245
HpyAV CCTTC 2 cut(s) 58, 250
HpyCH4V TGCA 3 cut(s) 106, 141, 287
HpyF10VI GCNNNNNNNGC 2 cut(s) 164, 293
HpyF3I CTNAG 1 cut(s) 93
Hsp92II CATG 1 cut(s) 248
HspAI GCGC 1 cut(s) 307
LmnI GCTCC 1 cut(s) 152
Lsp1109I GCAGC 4 cut(s) 167, 283, 286, 321
LweI GCATC 2 cut(s) 167, 193
MabI ACCWGGT 1 cut(s) 217
MaeI CTAG 2 cut(s) 171, 331
MaeIII GTNAC 1 cut(s) 208
MboII GAAGA 1 cut(s) 46
MseI TTAA 2 cut(s) 56, 198
MslI CAYNNNNRTG 2 cut(s) 21, 24
MspR9I CCNGG 3 cut(s) 189, 219, 304
Mva1269I GAATGC 2 cut(s) 240, 289
MvaI CCWGG 3 cut(s) 189, 219, 304
MwoI GCNNNNNNNGC 2 cut(s) 164, 293
NlaIII CATG 1 cut(s) 248
NlaIV GGNNCC 1 cut(s) 69
PagI TCATGA 1 cut(s) 244
PctI GAATGC 2 cut(s) 240, 289
PfeI GAWTC 1 cut(s) 37
PkrI GCNGC 4 cut(s) 157, 298, 301, 311
Psp6I CCWGG 3 cut(s) 187, 217, 302
PspGI CCWGG 3 cut(s) 187, 217, 302
PspN4I GGNNCC 1 cut(s) 69
PspPI GGNCC 1 cut(s) 67
PsrI GAACNNNNNNTAC 2 cut(s) 217, 249
RseI CAYNNNNRTG 2 cut(s) 21, 24
SaqAI TTAA 2 cut(s) 56, 198
SatI GCNGC 4 cut(s) 156, 297, 300, 310
Sau96I GGNCC 1 cut(s) 67
ScrFI CCNGG 3 cut(s) 189, 219, 304
SetI ASST 5 cut(s) 147, 193, 198, 220, 252
SexAI ACCWGGT 1 cut(s) 217
SfaNI GCATC 2 cut(s) 167, 193
SmiMI CAYNNNNRTG 2 cut(s) 21, 24
SpeI ACTAGT 1 cut(s) 170
SspI AATATT 1 cut(s) 276
SspMI CTAG 2 cut(s) 171, 331
StyD4I CCNGG 3 cut(s) 187, 217, 302
TfiI GAWTC 1 cut(s) 37
Tru1I TTAA 2 cut(s) 56, 198
Tru9I TTAA 2 cut(s) 56, 198
TseI GCWGC 4 cut(s) 155, 296, 299, 309
TspDTI ATGAA 3 cut(s) 47, 233, 296
XspI CTAG 2 cut(s) 171, 331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.