FvH4_7g09580

DnaJ central domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0015685
Physical Location & Seq
Reverse (-)
9201282 .. 9205472
4191 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g09580.t2

Sequence Viewer

Length: 1263 bp
ATGTTTCGCCATGCTGCGAGGAACAGTGATAACACCAAGTATTATGAGGTCCTTGGTGTCCCCAAAACTGCAAGTCAAGATGAGCTGAAGAAGGCTTATAAGAAAGCTGCTATCAAGAATCATCCTGATAAAGGCGGAGATCCTGAGAAGTTCAAGGAGTTAGGTCAAGCTTATGAAGTTCTTAATGATCCAGAGAAGAGACAAGTCTATGACCGATATGGTGAAGATGGTCTTAAAGATGGACCAGGACCAGGAGATGGTCGTCCCTCACATAATCCATTTGATATATTTGAAACGTTTTTGAATCCACGTCATCGTACACATGTAAGAAGGCAGAAAAAAGGTGAAGATGTGATCCATACCTTGAAGGTTTCTCTGGAGGAATTGTATAATAGCACAATAAAGAAACTCTCTCTCTCTAGGACTATTTTGTGTCCCAAATGTAAAGGGAAAGGCTCAAAGAGCGGGGCAACTGGGAGATGTTATGGATGCCATGGCACTGGAATGAAGGTTTCAACACAGTCAATTGGATTGGGCATGTTCCAACAGATGCAACATCTCTGCCCTGACTGTCGCGGCTCAGGTGAGGTAATCAGTGATAGAGATAAATGCCCACCATGCAAAGGAAACAAGATTACACAGGAAAAGAAGGTGCTGGATGTGCACATAGAGAAGGGAATGGAGCATGGACATAAAATTGTCTTTCAGGGACAGGCTGATGAAGCTCCTGATACAATTACCGGGGATATTGTAATCATAGTGCAACTCAAGGAGCACACCAAGTTTAAGCGGAAGTTTGATGATCTTTACGTAGAACACACTCTCAGTTTAACTGAGTCTCTGTGTGGGTTTCAGTTTGTGTTGACCCATCTTGATGGCAGGCAGCTTCTGGTCAAAACAAATCCTGGAGAAGTCATGAAACCTGGTCAATCGAAAGCAATCAATGATGAGGGCATGCCTCATCACCAACGGCCATTTATAAAAGGAATGCTTTACATCCATTTCAATGTAGAATTTCCAGATTCTGGGATTCTTTCCCCTGATCAATGCCGAAATTTAGCGAGAGTACTAACACCTAACCCAAGCGAGAAGCATCTGACTGACATGGAGCTTGATGAGTGCGAGGAAACCACCATGCATGATGTCAATATTGAGGATGAGATGAGGCGCAAGTCAAGACAACAGTACCGGGAGGCATATGACGACAACAACGACGATGATGATGATGACCCCATGCCACGGGTGCAGTGTGCCCAGCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
DnaJ DnaJ_C DnaJ_CXXCXGXG

Protein Analysis

421

Amino Acids

47.66

Weight (kDa)

6.56

Isoelectric Point (pI)

34.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 13 - 71 7.1e-23 DnaJ domain
DnaJ_C PF01556 120 - 306 3.3e-25 DnaJ C terminal domain
DnaJ_CXXCXGXG PF00684 145 - 211 2e-15 DnaJ central domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015685)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g09580
malus_domestica MD07G1100100.v1.1
prunus_persica Prupe.2G123700_v2.0.a1 Prupe.2G123700_v2.0.a1 Prupe.2G123700_v2.0.a1
pyrus_communis pycom07g08230
rosa_chinensis RchiOBHm_Chr1g0346511
rosa_laevigata RLG00000028797
rosa_multiflora Rmu_sc0001734.1_g000001
rosa_roxburghii Rroxscaffold_4G00308490
rosa_rugosa Rorug01G0180500 Rorug01G0180600
rosa_samantha Rh1BG166200 Rh1CG184500 Rh1DG197100
rosa_wichuraiana Rw1G016750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 99, 980
AccB7I CCANNNNNTGG 2 cut(s) 257, 1025
AccBSI CCGCTC 1 cut(s) 465
AccII CGCG 1 cut(s) 576
AciI CCGC 4 cut(s) 135, 465, 576, 790
AclI AACGTT 1 cut(s) 296
AclWI GGATC 3 cut(s) 134, 182, 349
AcoI YGGCCR 1 cut(s) 971
AcsI RAATTY 2 cut(s) 1013, 1054
AcuI CTGAAG 1 cut(s) 107
AfaI GTAC 3 cut(s) 319, 1068, 1187
AfiI CCNNNNNNNGG 6 cut(s) 131, 251, 257, 623, 1025, 1239
AflIII ACRYGT 1 cut(s) 322
AgsI TTSAA 6 cut(s) 154, 293, 304, 367, 516, 1006
AjiI CACGTC 1 cut(s) 311
AjnI CCWGG 4 cut(s) 244, 250, 904, 922
AluBI AGCT 6 cut(s) 85, 107, 170, 725, 886, 1111
AluI AGCT 6 cut(s) 85, 107, 170, 725, 886, 1111
Alw21I GWGCWC 2 cut(s) 666, 777
Alw26I GTCTC 2 cut(s) 193, 843
Alw44I GTGCAC 1 cut(s) 662
AlwI GGATC 3 cut(s) 134, 182, 349
AlwNI CAGNNNCTG 2 cut(s) 889, 1025
AoxI GGCC 1 cut(s) 971
ApaLI GTGCAC 1 cut(s) 662
ApeKI GCWGC 3 cut(s) 14, 107, 883
ApoI RAATTY 2 cut(s) 1013, 1054
AspLEI GCGC 1 cut(s) 1170
AspS9I GGNCC 3 cut(s) 49, 242, 248
AsuC2I CCSGG 2 cut(s) 742, 1190
AsuHPI GGTGA 4 cut(s) 233, 356, 596, 956
AvaII GGWCC 3 cut(s) 49, 242, 248
BaeGI GKGCMC 2 cut(s) 666, 1255
BaeI ACNNNNGTAYC 2 cut(s) 1169, 1202
Bbv12I GWGCWC 2 cut(s) 666, 777
BbvI GCAGC 2 cut(s) 94, 895
BccI CCATC 5 cut(s) 221, 233, 251, 869, 876
BceAI ACGGC 1 cut(s) 986
BciT130I CCWGG 4 cut(s) 246, 252, 906, 924
BclI TGATCA 1 cut(s) 1042
BcnI CCSGG 2 cut(s) 742, 1190
BcoDI GTCTC 2 cut(s) 193, 843
BfaI CTAG 1 cut(s) 420
BisI GCNGC 4 cut(s) 15, 108, 577, 884
BlsI GCNGC 4 cut(s) 16, 109, 578, 885
BmcAI AGTACT 1 cut(s) 1068
Bme1390I CCNGG 6 cut(s) 246, 252, 742, 906, 924, 1190
Bme18I GGWCC 3 cut(s) 49, 242, 248
BmgBI CACGTC 1 cut(s) 311
BmgT120I GGNCC 3 cut(s) 49, 242, 248
BmrFI CCNGG 6 cut(s) 246, 252, 742, 906, 924, 1190
BmrI ACTGGG 1 cut(s) 483
BmsI GCATC 3 cut(s) 479, 540, 1102
BmuI ACTGGG 1 cut(s) 483
BpmI CTGGAG 2 cut(s) 398, 927
Bpu10I CCTNAGC 1 cut(s) 580
BpuEI CTTGAG 1 cut(s) 752
BpuMI CCSGG 2 cut(s) 742, 1190
BsaAI YACGTR 1 cut(s) 811
BsaJI CCNNGG 4 cut(s) 52, 493, 741, 1238
BsaXI ACNNNNNCTCC 2 cut(s) 371, 401
Bsc4I CCNNNNNNNGG 6 cut(s) 131, 251, 257, 623, 1025, 1239
Bse1I ACTGG 2 cut(s) 478, 505
BseBI CCWGG 4 cut(s) 246, 252, 906, 924
BseDI CCNNGG 4 cut(s) 52, 493, 741, 1238
BseGI GGATG 5 cut(s) 121, 494, 664, 996, 1162
BseLI CCNNNNNNNGG 6 cut(s) 131, 251, 257, 623, 1025, 1239
BseMII CTCAG 4 cut(s) 135, 594, 825, 838
BseNI ACTGG 2 cut(s) 478, 505
BseSI GKGCMC 2 cut(s) 666, 1255
BseXI GCAGC 2 cut(s) 94, 895
BseYI CCCAGC 1 cut(s) 1254
Bsh1236I CGCG 1 cut(s) 576
BshFI GGCC 1 cut(s) 973
BsiHKAI GWGCWC 2 cut(s) 666, 777
BsiSI CCGG 2 cut(s) 741, 1189
BslFI GGGAC 4 cut(s) 44, 249, 420, 723
BslI CCNNNNNNNGG 6 cut(s) 131, 251, 257, 623, 1025, 1239
BsmAI GTCTC 2 cut(s) 193, 843
BsmFI GGGAC 4 cut(s) 44, 249, 420, 723
BsmI GAATGC 1 cut(s) 993
BsnI GGCC 1 cut(s) 973
Bsp1286I GDGCHC 3 cut(s) 666, 777, 1255
Bsp143I GATC 5 cut(s) 139, 187, 354, 802, 1042
Bsp19I CCATGG 1 cut(s) 493
BspACI CCGC 4 cut(s) 135, 465, 576, 790
BspANI GGCC 1 cut(s) 973
BspCNI CTCAG 4 cut(s) 136, 593, 826, 837
BspFNI CGCG 1 cut(s) 576
BspHI TCATGA 1 cut(s) 915
BspPI GGATC 3 cut(s) 134, 182, 349
BsrBI CCGCTC 1 cut(s) 465
BsrI ACTGG 2 cut(s) 478, 505
BssECI CCNNGG 4 cut(s) 52, 493, 741, 1238
BssMI GATC 5 cut(s) 139, 187, 354, 802, 1042
BssT1I CCWWGG 2 cut(s) 52, 493
Bst2UI CCWGG 4 cut(s) 246, 252, 906, 924
Bst4CI ACNGT 4 cut(s) 26, 522, 572, 1185
Bst6I CTCTTC 1 cut(s) 191
BstBAI YACGTR 1 cut(s) 811
BstC8I GCNNGC 2 cut(s) 881, 956
BstDEI CTNAG 4 cut(s) 144, 580, 824, 834
BstDSI CCRYGG 2 cut(s) 493, 1238
BstENI CCTNNNNNAGG 1 cut(s) 129
BstF5I GGATG 5 cut(s) 121, 494, 664, 996, 1162
BstFNI CGCG 1 cut(s) 576
BstHHI GCGC 1 cut(s) 1170
BstKTI GATC 5 cut(s) 142, 190, 357, 805, 1045
BstMAI GTCTC 2 cut(s) 193, 843
BstMBI GATC 5 cut(s) 139, 187, 354, 802, 1042
BstMWI GCNNNNNNNGC 5 cut(s) 462, 618, 661, 722, 1243
BstNI CCWGG 4 cut(s) 246, 252, 906, 924
BstNSI RCATGY 3 cut(s) 326, 541, 958
BstSCI CCNGG 6 cut(s) 244, 250, 740, 904, 922, 1188
BstSLI GKGCMC 2 cut(s) 666, 1255
BstSNI TACGTA 1 cut(s) 811
BstUI CGCG 1 cut(s) 576
BstV1I GCAGC 2 cut(s) 94, 895
BstX2I RGATCY 1 cut(s) 139
BstXI CCANNNNNNTGG 2 cut(s) 500, 875
BstYI RGATCY 1 cut(s) 139
BsuRI GGCC 1 cut(s) 973
BtgI CCRYGG 2 cut(s) 493, 1238
BtrI CACGTC 1 cut(s) 311
BtsCI GGATG 5 cut(s) 121, 494, 664, 996, 1162
BtsI GCAGTG 1 cut(s) 1253
BtsIMutI CAGTG 4 cut(s) 31, 498, 601, 1253
Cac8I GCNNGC 2 cut(s) 881, 956
CaiI CAGNNNCTG 2 cut(s) 889, 1025
CciI TCATGA 1 cut(s) 915
CfoI GCGC 1 cut(s) 1170
Cfr13I GGNCC 3 cut(s) 49, 242, 248
CsiI ACCWGGT 1 cut(s) 922
Csp6I GTAC 3 cut(s) 318, 1067, 1186
CviQI GTAC 3 cut(s) 318, 1067, 1186
DdeI CTNAG 4 cut(s) 144, 580, 824, 834
DpnI GATC 5 cut(s) 141, 189, 356, 804, 1044
DpnII GATC 5 cut(s) 139, 187, 354, 802, 1042
EaeI YGGCCR 1 cut(s) 971
Eam1104I CTCTTC 1 cut(s) 191
EarI CTCTTC 1 cut(s) 191
EciI GGCGGA 1 cut(s) 150
Eco105I TACGTA 1 cut(s) 811
Eco130I CCWWGG 2 cut(s) 52, 493
Eco47I GGWCC 3 cut(s) 49, 242, 248
Eco57I CTGAAG 1 cut(s) 107
EcoNI CCTNNNNNAGG 1 cut(s) 129
EcoO109I RGGNCCY 1 cut(s) 49
EcoRII CCWGG 4 cut(s) 244, 250, 904, 922
EcoT14I CCWWGG 2 cut(s) 52, 493
EcoT22I ATGCAT 1 cut(s) 1140
ErhI CCWWGG 2 cut(s) 52, 493
FalI AAGNNNNNCTT 4 cut(s) 216, 248, 975, 1007
FaqI GGGAC 4 cut(s) 44, 249, 420, 723
FauI CCCGC 1 cut(s) 458
FauNDI CATATG 1 cut(s) 1198
FbaI TGATCA 1 cut(s) 1042
Fnu4HI GCNGC 4 cut(s) 15, 108, 577, 884
FokI GGATG 5 cut(s) 108, 501, 671, 983, 1169
Fsp4HI GCNGC 4 cut(s) 15, 108, 577, 884
FspBI CTAG 1 cut(s) 420
GlaI GCGC 1 cut(s) 1169
GluI GCNGC 4 cut(s) 15, 108, 577, 884
GsaI CCCAGC 1 cut(s) 1258
GsuI CTGGAG 2 cut(s) 398, 927
HaeIII GGCC 1 cut(s) 973
HapII CCGG 2 cut(s) 741, 1189
HhaI GCGC 1 cut(s) 1170
Hin6I GCGC 1 cut(s) 1168
HinP1I GCGC 1 cut(s) 1168
HincII GTYRAC 1 cut(s) 864
HindII GTYRAC 1 cut(s) 864
HindIII AAGCTT 1 cut(s) 168
HinfI GANTC 5 cut(s) 118, 304, 836, 1022, 1030
HpaII CCGG 2 cut(s) 741, 1189
HphI GGTGA 4 cut(s) 233, 356, 596, 956
Hpy166II GTNNAC 3 cut(s) 320, 664, 864
Hpy188I TCNGA 1 cut(s) 1098
Hpy8I GTNNAC 3 cut(s) 320, 664, 864
Hpy99I CGWCG 1 cut(s) 1217
HpyAV CCTTC 6 cut(s) 85, 324, 361, 502, 643, 667
HpyCH4III ACNGT 4 cut(s) 26, 522, 572, 1185
HpyCH4IV ACGT 3 cut(s) 296, 310, 810
HpyCH4V TGCA 7 cut(s) 71, 553, 621, 664, 763, 1138, 1246
HpyF10VI GCNNNNNNNGC 5 cut(s) 462, 618, 661, 722, 1243
HpyF3I CTNAG 4 cut(s) 144, 580, 824, 834
HpySE526I ACGT 3 cut(s) 296, 310, 810
HspAI GCGC 1 cut(s) 1168
Ksp22I TGATCA 1 cut(s) 1042
Kzo9I GATC 5 cut(s) 139, 187, 354, 802, 1042
LmnI GCTCC 4 cut(s) 682, 730, 772, 1108
Lsp1109I GCAGC 2 cut(s) 94, 895
LweI GCATC 3 cut(s) 479, 540, 1102
MabI ACCWGGT 1 cut(s) 922
MaeI CTAG 1 cut(s) 420
MaeII ACGT 3 cut(s) 296, 310, 810
MalI GATC 5 cut(s) 141, 189, 356, 804, 1044
MbiI CCGCTC 1 cut(s) 465
MboI GATC 5 cut(s) 139, 187, 354, 802, 1042
MboII GAAGA 4 cut(s) 100, 208, 236, 359
MfeI CAATTG 1 cut(s) 525
MflI RGATCY 1 cut(s) 139
MhlI GDGCHC 3 cut(s) 666, 777, 1255
MluCI AATT 6 cut(s) 383, 525, 696, 735, 1013, 1054
MlyI GAGTC 1 cut(s) 845
MmeI TCCRAC 1 cut(s) 568
Mph1103I ATGCAT 1 cut(s) 1140
MseI TTAA 4 cut(s) 183, 234, 786, 830
MslI CAYNNNNRTG 3 cut(s) 503, 873, 1005
MspI CCGG 2 cut(s) 741, 1189
MspR9I CCNGG 6 cut(s) 246, 252, 742, 906, 924, 1190
MunI CAATTG 1 cut(s) 525
Mva1269I GAATGC 1 cut(s) 993
MvaI CCWGG 4 cut(s) 246, 252, 906, 924
MvnI CGCG 1 cut(s) 576
MwoI GCNNNNNNNGC 5 cut(s) 462, 618, 661, 722, 1243
NciI CCSGG 2 cut(s) 742, 1190
NcoI CCATGG 1 cut(s) 493
NdeI CATATG 1 cut(s) 1198
NdeII GATC 5 cut(s) 139, 187, 354, 802, 1042
NsiI ATGCAT 1 cut(s) 1140
NspI RCATGY 3 cut(s) 326, 541, 958
PaeI GCATGC 1 cut(s) 958
PagI TCATGA 1 cut(s) 915
PciI ACATGT 1 cut(s) 322
PcsI WCGNNNNNNNCGW 1 cut(s) 1209
PctI GAATGC 1 cut(s) 993
PfeI GAWTC 4 cut(s) 118, 304, 1022, 1030
PflMI CCANNNNNTGG 2 cut(s) 257, 1025
PfoI TCCNGGA 1 cut(s) 904
PkrI GCNGC 4 cut(s) 16, 109, 578, 885
PleI GAGTC 1 cut(s) 844
PpsI GAGTC 1 cut(s) 844
Ppu21I YACGTR 1 cut(s) 811
PpuMI RGGWCCY 1 cut(s) 49
PscI ACATGT 1 cut(s) 322
PsiI TTATAA 2 cut(s) 99, 980
Psp1406I AACGTT 1 cut(s) 296
Psp5II RGGWCCY 1 cut(s) 49
Psp6I CCWGG 4 cut(s) 244, 250, 904, 922
PspFI CCCAGC 1 cut(s) 1254
PspGI CCWGG 4 cut(s) 244, 250, 904, 922
PspPI GGNCC 3 cut(s) 49, 242, 248
PspPPI RGGWCCY 1 cut(s) 49
PstNI CAGNNNCTG 2 cut(s) 889, 1025
PsuI RGATCY 1 cut(s) 139
RsaI GTAC 3 cut(s) 319, 1068, 1187
RsaNI GTAC 3 cut(s) 318, 1067, 1186
RseI CAYNNNNRTG 3 cut(s) 503, 873, 1005
SaqAI TTAA 4 cut(s) 183, 234, 786, 830
SatI GCNGC 4 cut(s) 15, 108, 577, 884
Sau3AI GATC 5 cut(s) 139, 187, 354, 802, 1042
Sau96I GGNCC 3 cut(s) 49, 242, 248
ScaI AGTACT 1 cut(s) 1068
SchI GAGTC 1 cut(s) 845
ScrFI CCNGG 6 cut(s) 246, 252, 742, 906, 924, 1190
SduI GDGCHC 3 cut(s) 666, 777, 1255
SexAI ACCWGGT 1 cut(s) 922
SfaNI GCATC 3 cut(s) 479, 540, 1102
SinI GGWCC 3 cut(s) 49, 242, 248
SmiMI CAYNNNNRTG 3 cut(s) 503, 873, 1005
SmlI CTYRAG 1 cut(s) 767
SmoI CTYRAG 1 cut(s) 767
SnaBI TACGTA 1 cut(s) 811
SphI GCATGC 1 cut(s) 958
Sse9I AATT 6 cut(s) 383, 525, 696, 735, 1013, 1054
SsiI CCGC 4 cut(s) 135, 465, 576, 790
SspI AATATT 1 cut(s) 1150
SspMI CTAG 1 cut(s) 420
StyD4I CCNGG 6 cut(s) 244, 250, 740, 904, 922, 1188
StyI CCWWGG 2 cut(s) 52, 493
TaaI ACNGT 4 cut(s) 26, 522, 572, 1185
TaiI ACGT 3 cut(s) 299, 313, 813
TaqI TCGA 1 cut(s) 932
TaqII GACCGA 1 cut(s) 228
TasI AATT 6 cut(s) 383, 525, 696, 735, 1013, 1054
TatI WGTACW 1 cut(s) 1066
TauI GCSGC 1 cut(s) 579
TfiI GAWTC 4 cut(s) 118, 304, 1022, 1030
Tru1I TTAA 4 cut(s) 183, 234, 786, 830
Tru9I TTAA 4 cut(s) 183, 234, 786, 830
TscAI CASTG 4 cut(s) 31, 505, 601, 1253
TseI GCWGC 3 cut(s) 14, 107, 883
TspDTI ATGAA 4 cut(s) 189, 521, 735, 932
TspRI CASTG 4 cut(s) 31, 505, 601, 1253
Van91I CCANNNNNTGG 2 cut(s) 257, 1025
VneI GTGCAC 1 cut(s) 662
VpaK11BI GGWCC 3 cut(s) 49, 242, 248
XagI CCTNNNNNAGG 1 cut(s) 129
XapI RAATTY 2 cut(s) 1013, 1054
XceI RCATGY 3 cut(s) 326, 541, 958
XspI CTAG 1 cut(s) 420
ZrmI AGTACT 1 cut(s) 1068
Zsp2I ATGCAT 1 cut(s) 1140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.