RLG00000028797

DnaJ central domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0015685
Physical Location & Seq
Forward (+)
28141106 .. 28146632
5527 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028797

Sequence Viewer

Length: 1461 bp
ATGGGAGAAGGATTGGATCTTGGAGAAAGCTCTAAGAAATCAAAAGACAAAGAGAACATGAGGAAGAAGAAGAAATCAGAAGCTCAGATCGGAAAAGAAAGCATCGGCCTATCTTCCGATTTCAAACCCATAATCCCCGCCCTGTCGTTGTTGCACCCTTCATCTTTCGCCTTCTTCCGCCTCCACCGCCGCTTCTTCTTATTCCTTTCTCTTCCCTTCTTCTACCTCCACTTCCAAAGTAGCAGTAGTATCGAAGTGCTTCAATCATGTCAAGATGAGCTCAAGAAGGCATATAAGAAAGCTGCTATCAAGAATCATCCTGACAAAGGTGGAGATCCTGAAAAGTTCAAGGAGTTAGGTCAAGCTTATGAAGTTCTTAATGATCCAGAAAAGAGAGAAGTCTATGACCAATATGGTGAAGATGGTCTTAAAGACGGACCAGGACGTGGTGGTCCCTCACATAATCCATGTGATATATTTGAAACGTTTTTGAATCCACGTTATCGTACACATGTAAGAAGGCAGAAACAAGGTGAAGATGTGGTCCATACCTTAAAGGTTTCTTTGGAGGACTTGTATAATGGCACAACAAAGAAACTCTCTCTCTCTAGGAATATTTTGTGTCCCAAATGTAAAGGGAAAGGCTCAAAGAGCGGAGCAACAGGGAGCTGTTACGGATGCCGTGGTACTGGAATGAAGGTTTCAACACGGTCAGTTGGATTGGGCATGTTTCAACAGATGCAACATCTCTGCCCTGACTGCCGCGGCTCTGGTGAGGTAATCAGTGATAGAGATAAATGCCCACCATGCAAAGGAAACAAGATTAGTCACGAAAAGAAGGTGCTGGATGTGCACATAGAGAAGGGAATGGAGCATGGACAGAAAATTGTCTTTGAGGGACAGGCTGATGAAGCTCCTGATACAATTACCGGGGATATTGTTATCATAGTGCAACTCAAGGAGCACACCAAGTTCAAGCGGAAGTTTGATGATCTTTACATAGAACACACACTCAGTTTAACTGAGGCTCTGTGTGGGTTTCAGTTTGTGTTGACTCATCTTGATGGCAGGCCGCTTCTGGTCAAATCAAATCCTGGAGAAGTCATGAAGCCTGGTCAATCCAAAGCAATCAATGATGAGGGCATGCCGCATCACAAAAGGCCATTCATAAAGGGCATGCTTTTCATCCATTTCAACGTAGAATTTCCAGATTCTGGGATTCTTTCCCCTGATCAATGCCGAAATATAGCGACAGTACTAACACCCAAGCCAAGCAAGAAGCATTTGACTGACATGGAGCTTGATGAGTGTGAGGAAACCACTATGCATGATGTCAATATTGAGGATGAGATGAGGCGCAAGTCAAAACAACAGTACCGGGAGGCATATGATGATGATGATGATGACGATGATGAAGATGATGATGACCCCATGCCACGGGTGCAGTGTGCCCAGCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
DnaJ DnaJ_C DnaJ_CXXCXGXG

Protein Analysis

487

Amino Acids

54.93

Weight (kDa)

6.46

Isoelectric Point (pI)

39.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 91 - 136 3.3e-15 DnaJ domain
DnaJ_C PF01556 182 - 403 1.5e-36 DnaJ C terminal domain
DnaJ_CXXCXGXG PF00684 208 - 274 1e-14 DnaJ central domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015685)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g09580
malus_domestica MD07G1100100.v1.1
prunus_persica Prupe.2G123700_v2.0.a1 Prupe.2G123700_v2.0.a1 Prupe.2G123700_v2.0.a1
pyrus_communis pycom07g08230
rosa_chinensis RchiOBHm_Chr1g0346511
rosa_laevigata RLG00000028797
rosa_multiflora Rmu_sc0001734.1_g000001
rosa_roxburghii Rroxscaffold_4G00308490
rosa_rugosa Rorug01G0180500 Rorug01G0180600
rosa_samantha Rh1BG166200 Rh1CG184500 Rh1DG197100
rosa_wichuraiana Rw1G016750

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 450
AccB7I CCANNNNNTGG 2 cut(s) 446, 1214
AccBSI CCGCTC 1 cut(s) 654
AccII CGCG 1 cut(s) 765
AclI AACGTT 1 cut(s) 485
AclWI GGATC 3 cut(s) 24, 329, 377
AcsI RAATTY 1 cut(s) 1202
AfaI GTAC 4 cut(s) 508, 688, 1257, 1376
AfiI CCNNNNNNNGG 5 cut(s) 326, 446, 812, 1214, 1437
AflIII ACRYGT 1 cut(s) 511
AgsI TTSAA 9 cut(s) 124, 263, 349, 482, 493, 705, 734, 976, 1195
AjiI CACGTC 1 cut(s) 446
AjnI CCWGG 3 cut(s) 439, 1093, 1111
AluBI AGCT 8 cut(s) 30, 83, 280, 302, 365, 669, 914, 1300
AluI AGCT 8 cut(s) 30, 83, 280, 302, 365, 669, 914, 1300
Alw21I GWGCWC 3 cut(s) 282, 855, 966
Alw44I GTGCAC 1 cut(s) 851
AlwI GGATC 3 cut(s) 24, 329, 377
AlwNI CAGNNNCTG 1 cut(s) 1214
AoxI GGCC 3 cut(s) 106, 1070, 1160
ApaLI GTGCAC 1 cut(s) 851
ApeKI GCWGC 1 cut(s) 302
ApoI RAATTY 1 cut(s) 1202
Asp700I GAANNNNTTC 1 cut(s) 258
AspLEI GCGC 1 cut(s) 1359
AspS9I GGNCC 3 cut(s) 437, 452, 544
AsuC2I CCSGG 2 cut(s) 931, 1379
AsuHPI GGTGA 3 cut(s) 428, 545, 785
AvaII GGWCC 3 cut(s) 437, 452, 544
BaeGI GKGCMC 2 cut(s) 855, 1453
BaeI ACNNNNGTAYC 2 cut(s) 1358, 1391
BanII GRGCYC 1 cut(s) 282
Bbv12I GWGCWC 3 cut(s) 282, 855, 966
BbvI GCAGC 1 cut(s) 289
BccI CCATC 2 cut(s) 416, 1058
BceAI ACGGC 1 cut(s) 666
BcgI CGANNNNNNTGC 2 cut(s) 232, 266
BciT130I CCWGG 3 cut(s) 441, 1095, 1113
BclI TGATCA 1 cut(s) 1231
BcnI CCSGG 2 cut(s) 931, 1379
BfaI CTAG 1 cut(s) 609
BisI GCNGC 6 cut(s) 190, 303, 763, 766, 1073, 1148
BlsI GCNGC 6 cut(s) 191, 304, 764, 767, 1074, 1149
BmcAI AGTACT 1 cut(s) 1257
Bme1390I CCNGG 5 cut(s) 441, 931, 1095, 1113, 1379
Bme18I GGWCC 3 cut(s) 437, 452, 544
BmgBI CACGTC 1 cut(s) 446
BmgT120I GGNCC 3 cut(s) 437, 452, 544
BmiI GGNNCC 1 cut(s) 454
BmrFI CCNGG 5 cut(s) 441, 931, 1095, 1113, 1379
BmsI GCATC 4 cut(s) 111, 668, 729, 1159
BpmI CTGGAG 1 cut(s) 1116
BpuEI CTTGAG 2 cut(s) 266, 941
BpuMI CCSGG 2 cut(s) 931, 1379
BsaJI CCNNGG 4 cut(s) 682, 763, 930, 1436
BsaXI ACNNNNNCTCC 2 cut(s) 560, 590
Bsc4I CCNNNNNNNGG 5 cut(s) 326, 446, 812, 1214, 1437
Bse1I ACTGG 1 cut(s) 694
BseBI CCWGG 3 cut(s) 441, 1095, 1113
BseDI CCNNGG 4 cut(s) 682, 763, 930, 1436
BseGI GGATG 5 cut(s) 316, 683, 853, 1185, 1351
BseLI CCNNNNNNNGG 5 cut(s) 326, 446, 812, 1214, 1437
BseMII CTCAG 3 cut(s) 98, 1014, 1027
BseNI ACTGG 1 cut(s) 694
BseSI GKGCMC 2 cut(s) 855, 1453
BseXI GCAGC 1 cut(s) 289
BseYI CCCAGC 1 cut(s) 1452
Bsh1236I CGCG 1 cut(s) 765
BshFI GGCC 3 cut(s) 108, 1072, 1162
BsiHKAI GWGCWC 3 cut(s) 282, 855, 966
BsiSI CCGG 2 cut(s) 930, 1378
BslFI GGGAC 3 cut(s) 438, 609, 912
BslI CCNNNNNNNGG 5 cut(s) 326, 446, 812, 1214, 1437
BsmFI GGGAC 3 cut(s) 438, 609, 912
BsnI GGCC 3 cut(s) 108, 1072, 1162
Bsp1286I GDGCHC 4 cut(s) 282, 855, 966, 1453
Bsp143I GATC 6 cut(s) 16, 87, 334, 382, 991, 1231
BspANI GGCC 3 cut(s) 108, 1072, 1162
BspCNI CTCAG 3 cut(s) 97, 1015, 1026
BspFNI CGCG 1 cut(s) 765
BspHI TCATGA 1 cut(s) 1104
BspLI GGNNCC 1 cut(s) 454
BspPI GGATC 3 cut(s) 24, 329, 377
BsrBI CCGCTC 1 cut(s) 654
BsrI ACTGG 1 cut(s) 694
BssECI CCNNGG 4 cut(s) 682, 763, 930, 1436
BssMI GATC 6 cut(s) 16, 87, 334, 382, 991, 1231
Bst2UI CCWGG 3 cut(s) 441, 1095, 1113
Bst4CI ACNGT 3 cut(s) 711, 1255, 1374
Bst6I CTCTTC 1 cut(s) 216
BstC8I GCNNGC 3 cut(s) 1070, 1145, 1178
BstDEI CTNAG 4 cut(s) 33, 84, 1013, 1023
BstDSI CCRYGG 3 cut(s) 682, 763, 1436
BstENI CCTNNNNNAGG 1 cut(s) 324
BstF5I GGATG 5 cut(s) 316, 683, 853, 1185, 1351
BstFNI CGCG 1 cut(s) 765
BstHHI GCGC 1 cut(s) 1359
BstKTI GATC 6 cut(s) 19, 90, 337, 385, 994, 1234
BstMBI GATC 6 cut(s) 16, 87, 334, 382, 991, 1231
BstMWI GCNNNNNNNGC 7 cut(s) 186, 651, 759, 807, 850, 911, 1441
BstNI CCWGG 3 cut(s) 441, 1095, 1113
BstNSI RCATGY 4 cut(s) 515, 730, 1147, 1180
BstSCI CCNGG 5 cut(s) 439, 929, 1093, 1111, 1377
BstSLI GKGCMC 2 cut(s) 855, 1453
BstUI CGCG 1 cut(s) 765
BstV1I GCAGC 1 cut(s) 289
BstX2I RGATCY 2 cut(s) 16, 334
BstYI RGATCY 2 cut(s) 16, 334
BsuRI GGCC 3 cut(s) 108, 1072, 1162
BtgI CCRYGG 3 cut(s) 682, 763, 1436
BtrI CACGTC 1 cut(s) 446
BtsCI GGATG 5 cut(s) 316, 683, 853, 1185, 1351
BtsI GCAGTG 1 cut(s) 1451
BtsIMutI CAGTG 2 cut(s) 790, 1451
Cac8I GCNNGC 3 cut(s) 1070, 1145, 1178
CaiI CAGNNNCTG 1 cut(s) 1214
CciI TCATGA 1 cut(s) 1104
CfoI GCGC 1 cut(s) 1359
Cfr13I GGNCC 3 cut(s) 437, 452, 544
Cfr42I CCGCGG 1 cut(s) 766
Csp6I GTAC 4 cut(s) 507, 687, 1256, 1375
CviQI GTAC 4 cut(s) 507, 687, 1256, 1375
DdeI CTNAG 4 cut(s) 33, 84, 1013, 1023
DpnI GATC 6 cut(s) 18, 89, 336, 384, 993, 1233
DpnII GATC 6 cut(s) 16, 87, 334, 382, 991, 1231
DrdI GACNNNNNNGTC 1 cut(s) 450
DseDI GACNNNNNNGTC 1 cut(s) 450
Eam1104I CTCTTC 1 cut(s) 216
EarI CTCTTC 1 cut(s) 216
EciI GGCGGA 1 cut(s) 167
Ecl136II GAGCTC 1 cut(s) 280
Eco24I GRGCYC 1 cut(s) 282
Eco47I GGWCC 3 cut(s) 437, 452, 544
Eco53kI GAGCTC 1 cut(s) 280
EcoICRI GAGCTC 1 cut(s) 280
EcoNI CCTNNNNNAGG 1 cut(s) 324
EcoRII CCWGG 3 cut(s) 439, 1093, 1111
EcoT22I ATGCAT 1 cut(s) 1329
EcoT38I GRGCYC 1 cut(s) 282
FalI AAGNNNNNCTT 2 cut(s) 411, 443
FaqI GGGAC 3 cut(s) 438, 609, 912
FauI CCCGC 1 cut(s) 145
FauNDI CATATG 1 cut(s) 1387
FbaI TGATCA 1 cut(s) 1231
Fnu4HI GCNGC 6 cut(s) 190, 303, 763, 766, 1073, 1148
FokI GGATG 5 cut(s) 303, 690, 860, 1172, 1358
FriOI GRGCYC 1 cut(s) 282
Fsp4HI GCNGC 6 cut(s) 190, 303, 763, 766, 1073, 1148
FspBI CTAG 1 cut(s) 609
GlaI GCGC 1 cut(s) 1358
GluI GCNGC 6 cut(s) 190, 303, 763, 766, 1073, 1148
GsaI CCCAGC 1 cut(s) 1456
GsuI CTGGAG 1 cut(s) 1116
HaeIII GGCC 3 cut(s) 108, 1072, 1162
HapII CCGG 2 cut(s) 930, 1378
HhaI GCGC 1 cut(s) 1359
Hin6I GCGC 1 cut(s) 1357
HinP1I GCGC 1 cut(s) 1357
HincII GTYRAC 1 cut(s) 1053
HindII GTYRAC 1 cut(s) 1053
HindIII AAGCTT 1 cut(s) 363
HinfI GANTC 5 cut(s) 313, 493, 1054, 1211, 1219
HpaII CCGG 2 cut(s) 930, 1378
HphI GGTGA 3 cut(s) 428, 545, 785
Hpy166II GTNNAC 3 cut(s) 509, 853, 1053
Hpy188I TCNGA 4 cut(s) 79, 87, 92, 118
Hpy8I GTNNAC 3 cut(s) 509, 853, 1053
HpyAV CCTTC 8 cut(s) 168, 181, 226, 280, 513, 691, 832, 856
HpyCH4III ACNGT 3 cut(s) 711, 1255, 1374
HpyCH4IV ACGT 4 cut(s) 445, 485, 499, 1197
HpyCH4V TGCA 7 cut(s) 154, 742, 810, 853, 952, 1327, 1444
HpyF10VI GCNNNNNNNGC 7 cut(s) 186, 651, 759, 807, 850, 911, 1441
HpyF3I CTNAG 4 cut(s) 33, 84, 1013, 1023
HpySE526I ACGT 4 cut(s) 445, 485, 499, 1197
HspAI GCGC 1 cut(s) 1357
Ksp22I TGATCA 1 cut(s) 1231
KspI CCGCGG 1 cut(s) 766
Kzo9I GATC 6 cut(s) 16, 87, 334, 382, 991, 1231
LmnI GCTCC 6 cut(s) 656, 666, 871, 919, 961, 1297
Lsp1109I GCAGC 1 cut(s) 289
LweI GCATC 4 cut(s) 111, 668, 729, 1159
MaeI CTAG 1 cut(s) 609
MaeII ACGT 4 cut(s) 445, 485, 499, 1197
MaeIII GTNAC 2 cut(s) 671, 827
MalI GATC 6 cut(s) 18, 89, 336, 384, 993, 1233
MbiI CCGCTC 1 cut(s) 654
MboI GATC 6 cut(s) 16, 87, 334, 382, 991, 1231
MflI RGATCY 2 cut(s) 16, 334
MhlI GDGCHC 4 cut(s) 282, 855, 966, 1453
MluCI AATT 3 cut(s) 885, 924, 1202
MlyI GAGTC 1 cut(s) 1048
MmeI TCCRAC 1 cut(s) 697
Mph1103I ATGCAT 1 cut(s) 1329
MroXI GAANNNNTTC 1 cut(s) 258
MseI TTAA 4 cut(s) 378, 429, 554, 1019
MslI CAYNNNNRTG 1 cut(s) 1062
MspA1I CMGCKG 1 cut(s) 765
MspI CCGG 2 cut(s) 930, 1378
MspR9I CCNGG 5 cut(s) 441, 931, 1095, 1113, 1379
MvaI CCWGG 3 cut(s) 441, 1095, 1113
MvnI CGCG 1 cut(s) 765
MwoI GCNNNNNNNGC 7 cut(s) 186, 651, 759, 807, 850, 911, 1441
NciI CCSGG 2 cut(s) 931, 1379
NdeI CATATG 1 cut(s) 1387
NdeII GATC 6 cut(s) 16, 87, 334, 382, 991, 1231
NlaIV GGNNCC 1 cut(s) 454
NmuCI GTSAC 1 cut(s) 827
NsiI ATGCAT 1 cut(s) 1329
NspI RCATGY 4 cut(s) 515, 730, 1147, 1180
PaeI GCATGC 2 cut(s) 1147, 1180
PagI TCATGA 1 cut(s) 1104
PciI ACATGT 1 cut(s) 511
PdmI GAANNNNTTC 1 cut(s) 258
PfeI GAWTC 4 cut(s) 313, 493, 1211, 1219
PflMI CCANNNNNTGG 2 cut(s) 446, 1214
PfoI TCCNGGA 1 cut(s) 1093
PkrI GCNGC 6 cut(s) 191, 304, 764, 767, 1074, 1149
PleI GAGTC 1 cut(s) 1048
PpsI GAGTC 1 cut(s) 1048
PscI ACATGT 1 cut(s) 511
Psp124BI GAGCTC 1 cut(s) 282
Psp1406I AACGTT 1 cut(s) 485
Psp6I CCWGG 3 cut(s) 439, 1093, 1111
PspFI CCCAGC 1 cut(s) 1452
PspGI CCWGG 3 cut(s) 439, 1093, 1111
PspN4I GGNNCC 1 cut(s) 454
PspPI GGNCC 3 cut(s) 437, 452, 544
PstNI CAGNNNCTG 1 cut(s) 1214
PsuI RGATCY 2 cut(s) 16, 334
RsaI GTAC 4 cut(s) 508, 688, 1257, 1376
RsaNI GTAC 4 cut(s) 507, 687, 1256, 1375
RseI CAYNNNNRTG 1 cut(s) 1062
SacI GAGCTC 1 cut(s) 282
SacII CCGCGG 1 cut(s) 766
SaqAI TTAA 4 cut(s) 378, 429, 554, 1019
SatI GCNGC 6 cut(s) 190, 303, 763, 766, 1073, 1148
Sau3AI GATC 6 cut(s) 16, 87, 334, 382, 991, 1231
Sau96I GGNCC 3 cut(s) 437, 452, 544
ScaI AGTACT 1 cut(s) 1257
SchI GAGTC 1 cut(s) 1048
ScrFI CCNGG 5 cut(s) 441, 931, 1095, 1113, 1379
SduI GDGCHC 4 cut(s) 282, 855, 966, 1453
SfaNI GCATC 4 cut(s) 111, 668, 729, 1159
Sfr303I CCGCGG 1 cut(s) 766
SgrBI CCGCGG 1 cut(s) 766
SinI GGWCC 3 cut(s) 437, 452, 544
SmiMI CAYNNNNRTG 1 cut(s) 1062
SmlI CTYRAG 2 cut(s) 281, 956
SmoI CTYRAG 2 cut(s) 281, 956
SphI GCATGC 2 cut(s) 1147, 1180
Sse9I AATT 3 cut(s) 885, 924, 1202
SspI AATATT 2 cut(s) 616, 1339
SspMI CTAG 1 cut(s) 609
SstI GAGCTC 1 cut(s) 282
StyD4I CCNGG 5 cut(s) 439, 929, 1093, 1111, 1377
TaaI ACNGT 3 cut(s) 711, 1255, 1374
TaiI ACGT 4 cut(s) 448, 488, 502, 1200
TaqI TCGA 1 cut(s) 252
TasI AATT 3 cut(s) 885, 924, 1202
TatI WGTACW 1 cut(s) 1255
TauI GCSGC 5 cut(s) 192, 765, 768, 1075, 1150
TfiI GAWTC 4 cut(s) 313, 493, 1211, 1219
Tru1I TTAA 4 cut(s) 378, 429, 554, 1019
Tru9I TTAA 4 cut(s) 378, 429, 554, 1019
TscAI CASTG 2 cut(s) 790, 1451
TseFI GTSAC 1 cut(s) 827
TseI GCWGC 1 cut(s) 302
Tsp45I GTSAC 1 cut(s) 827
TspDTI ATGAA 8 cut(s) 150, 384, 710, 924, 1121, 1156, 1174, 1428
TspGWI ACGGA 2 cut(s) 450, 690
TspRI CASTG 2 cut(s) 790, 1451
Van91I CCANNNNNTGG 2 cut(s) 446, 1214
VneI GTGCAC 1 cut(s) 851
VpaK11BI GGWCC 3 cut(s) 437, 452, 544
XagI CCTNNNNNAGG 1 cut(s) 324
XapI RAATTY 1 cut(s) 1202
XceI RCATGY 4 cut(s) 515, 730, 1147, 1180
XmnI GAANNNNTTC 1 cut(s) 258
XspI CTAG 1 cut(s) 609
ZrmI AGTACT 1 cut(s) 1257
Zsp2I ATGCAT 1 cut(s) 1329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.