Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb7

Orthogroup ID

OG0012306

Physical Location & Seq

Genomic Coordinates Forward (+)

9897368 .. 9906136

Gene Structure 8769 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_7g10500.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2241 bp

ATGCCGCGCTTCCCGCGCTTCGCCACCACACGCCTCCTCTACTCCCTCCACGCCTCCAAAACGACGCCGTCCCCTTCCCCTTCCTCCTCCTCGCTCCTCCTCGGCTCGTTCCACCTCCGCCAATTCTCCGCCGGCAACAGCCTCGCGCGCGCCAAGGACGACAAGGACGTATGGTGGACGGAGGTCATGGCGAAGCTCCGCAACATCGGAATCTCGGCGCATATCGACTCCGGCAAGACGACTCTCACCGAGAGGATTCTATACTACACCGGAAAGATCCACGAGATTCACGAGGTCCGGGGACGCGACGGCGTCGGCGCTAAAATGGACTCCATGGATTTGGAGCGGGAGAAGGGAATTACCATTCAGTCTGCGGCGACTTACTGTACTTGGAATGGTTATCAGATTAACATAATTGACACCCCTGGTCACGTTGATTTCACCATTGAGGTTGAGAGAGCTTTGCGTGTTCTTGATGGAGCCATCCTTGTCCTTTGTAGCGTTGGTGGTGTACAGAGTCAGTCAATTACTGTTGATCGGCAAATGAAAAGATATGAGGTTCCAAGAATAGCATTTATAAACAAACTTGATCGAATGGGAGCAGATCCATGGAAAGTTTTGAATCAGATGAGGTCGAAGCTCCGGCATCATGCTGCTGCATTGCAACTTCCTATTGGGTTGGAAGATGATTTTAAGGGTCTCATTGATCTTGTGCAGTTAAAAGCGTTGTATTTTCACGGGTCTAGCGGTCAGGAAATTGTCATTGAAGATATTCCTTCTGATATGGAGGTGTTAGTCGCAGAAAAAAGGCGTGAGCTAATTGAAATTGTATCCGAAGTTGATGACCAACTTGCTGAAGCATTTCTTGAAGATGTGCCTATTACATCTACTGATCTGGAGGAAGCTATACGAAGGGCTACCATTGCAAGGAAATTTATACCTGTGTTCATGGGCAGTGCATTTAAAAACAAGGGTGTACAACCACTTTTGAATGGCGTACTTGGTTATTTGCCTTGTCCAACTGAAGTCAGTAACTATGCTCTTGACCAGACTAAGGATGAAGAGAAGGTTATATTAGGTGGAACTCCAGATGGACCTCTTGTAGCATTAGCCTTTAAATTGGAGGAAGGGCGTTTTGGTCAGTTAACCTTTCTAAGAATCTATGAAGGTGTTATACGGAAGGGTGATTTCATTTTTAACATTAACACTGGCAAGAAGCTTAAGGTTCCTCGCTTGGTCCGGATGCATTCCGATGAGATGGAGGATATTCAAGAGGCGCATGCTGGGCAAATAGTTGCTGTATTTGGTGTGGATTGTGCATCAGGAGATACATTTACTGATGGTTCAGTTAAATATACCATGACTTCTATGAATGTCCCTGAGCCAGTGATGTCATTAGCAGTTCAGCCAGTTTCAAAAGATTCAGGAGGCCAAAAAGAAGATCCTACCTTCCGAGTTGGCTTGGACCGTGAGAGTGGACAGACAATCATATCGGGAATGGGAGAGCTGCATTTGGAAGTTTATGTTGAACGCATTCGGAGAGAATATAAGGTTGATGCAACTGTTGGAAAGCCTCGTGTAAACTTCAGAGAGACTGTTACTCAACGTGCCGACTTTGATTATTTACATAAAAAGCAATCTGGAGGACAGGGTCAATATGGGCGAGTAACTGGATTTATCGAACCACTGCCTGCAGGATCAATCACAAAGTTTGAATTTGAAAACAGTATTGTGGGACAAGCTATACCATCAAATTTTATCCCAGCCATTGAGAAGGGTTTCAAAGAAGCAGCCAATTCGGGCTCATTAATTGGCCATCCAGTTGAAAATGTTCGCATTGTTTTAACTGATGGTGCTTCCCATGCTGTGGATTCTAGTGAACTTGCCTTCAAATTAGCTGCAATATATGCCTTTAGAAAGTGCTATACGGTAGCAAAGCCGGTGATACTGGAGCCTGTTATGTTGGTGGAGTTGAAAGTACCTACAGAATTTCAGGGTACTGTTGCTGGTGATATTAACAAGAGGAAAGGTGTTATTATTGGAAACGATCAGGAGGGGGATGACTCTGTTATTACAGCCCAAGTCCCTTTGAACAATATGTTTGGCTATTCCACATCTCTTCGATCGATGACACAGGGAAAAGGTGAATTCACAATGGAGTACAAAGAGCATTCTCCCGTTTCTCATGATGTGCAGGCGCAGTTAGTAAACAACTACAAGGGACCTAATGCCACTGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003676 GO:0003723 GO:0003746 GO:0003824 GO:0003924 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006412 GO:0006414 GO:0006518 GO:0006807 GO:0008135 GO:0008144 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019538 GO:0030554 GO:0032543 GO:0032553 GO:0032555 GO:0032559 GO:0034641 GO:0034645 GO:0035639 GO:0036094 GO:0042221 GO:0043043 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0050896 GO:0070125 GO:0071704 GO:0097159 GO:0097367 GO:0140053 GO:1901265 GO:1901363 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EFG_C EFG_II EFG_IV GTP_EFTU GTP_EFTU_D2

Protein Analysis

747

Amino Acids

82.48

Weight (kDa)

5.88

Isoelectric Point (pI)

37.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GTP_EFTU	PF00009	65 - 338	8.9e-61	Elongation factor Tu GTP binding domain
EF-G_D2	PF22042	379 - 447	1.4e-10	Elongation factor G domain 2
GTP_EFTU_D2	PF03144	380 - 446	8.7e-14	Elongation factor Tu domain 2
EFG_III	PF14492	459 - 533	3.2e-29	Elongation Factor G, domain III
EFG_IV	PF03764	534 - 654	1.3e-37	Elongation factor G, domain IV
EFG_C	PF00679	658 - 743	7.8e-26	Elongation factor G C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0012306)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G45332 AT1G45332 AT2G45030
fragaria_vesca	FvH4_7g10500 FvH4_7g10500 FvH4_7g10500
malus_domestica	MD07G1111000.v1.1
prunus_persica	Prupe.2G134500_v2.0.a1
pyrus_communis	pycom07g09580
rosa_chinensis	RchiOBHm_Chr1g0348291
rosa_laevigata	RLG00000028669
rosa_multiflora	Rmu_sc0027364.1_g000002
rosa_roxburghii	Rroxscaffold_4G00307040
rosa_rugosa	Rorug01G0194100
rosa_samantha	Rh1AG211300 Rh1BG177800 Rh1CG195700 Rh1DG207300
rosa_wichuraiana	Rw1G017850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	578
AccB7I	CCANNNNNTGG	1 cut(s)	1867
AccBSI	CCGCTC	1 cut(s)	346
AccII	CGCG	6 cut(s)	7, 16, 146, 148, 150, 306
AccIII	TCCGGA	1 cut(s)	1239
AciI	CCGC	8 cut(s)	5, 14, 118, 129, 199, 346, 374, 747
AclWI	GGATC	4 cut(s)	271, 599, 1436, 1705
AcoI	YGGCCR	1 cut(s)	1813
AcsI	RAATTY	5 cut(s)	932, 1715, 1753, 1988, 2147
AcuI	CTGAAG	3 cut(s)	876, 1044, 1570
AcyI	GRCGYC	2 cut(s)	65, 312
AfaI	GTAC	7 cut(s)	388, 513, 978, 999, 1980, 1999, 2162
AfiI	CCNNNNNNNGG	3 cut(s)	927, 1054, 1867
AflII	CTTAAG	1 cut(s)	1220
AjnI	CCWGG	1 cut(s)	424
AjuI	GAANNNNNNNTTGG	2 cut(s)	1119, 1151
AluBI	AGCT	9 cut(s)	196, 461, 640, 817, 905, 1219, 1507, 1742, 1898
AluI	AGCT	9 cut(s)	196, 461, 640, 817, 905, 1219, 1507, 1742, 1898
Alw26I	GTCTC	2 cut(s)	704, 1586
AlwI	GGATC	4 cut(s)	271, 599, 1436, 1705
Aor13HI	TCCGGA	1 cut(s)	1239
AoxI	GGCC	2 cut(s)	1429, 1813
ApeKI	GCWGC	5 cut(s)	653, 656, 1507, 1790, 1898
ApoI	RAATTY	5 cut(s)	932, 1715, 1753, 1988, 2147
AseI	ATTAAT	1 cut(s)	1808
Asp700I	GAANNNNTTC	5 cut(s)	771, 861, 1533, 1778, 1830
AspLEI	GCGC	9 cut(s)	9, 18, 148, 150, 152, 220, 320, 1279, 2200
AspS9I	GGNCC	5 cut(s)	295, 1094, 1237, 1465, 2222
AsuC2I	CCSGG	1 cut(s)	299
AsuHPI	GGTGA	6 cut(s)	238, 433, 1196, 1954, 2021, 2156
AvaII	GGWCC	5 cut(s)	295, 1094, 1237, 1465, 2222
BalI	TGGCCA	1 cut(s)	1815
BanII	GRGCYC	1 cut(s)	1805
BarI	GAAGNNNNNNTAC	2 cut(s)	1158, 1190
BauI	CACGAG	3 cut(s)	281, 290, 1575
BbvI	GCAGC	5 cut(s)	640, 643, 1494, 1802, 1885
BccI	CCATC	8 cut(s)	470, 491, 1085, 1252, 1334, 1756, 1824, 1844
BceAI	ACGGC	2 cut(s)	52, 325
BcgI	CGANNNNNNTGC	4 cut(s)	124, 158, 1779, 1813
BciT130I	CCWGG	1 cut(s)	426
BciVI	GTATCC	1 cut(s)	841
BcnI	CCSGG	1 cut(s)	299
BcoDI	GTCTC	2 cut(s)	704, 1586
BfaI	CTAG	2 cut(s)	744, 1875
BfmI	CTRYAG	2 cut(s)	1692, 1983
BfoI	RGCGCY	1 cut(s)	321
BfrI	CTTAAG	1 cut(s)	1220
BfuI	GTATCC	1 cut(s)	841
BisI	GCNGC	7 cut(s)	5, 375, 654, 657, 1508, 1791, 1899
BlsI	GCNGC	7 cut(s)	6, 376, 655, 658, 1509, 1792, 1900
Bme1390I	CCNGG	2 cut(s)	299, 426
Bme18I	GGWCC	5 cut(s)	295, 1094, 1237, 1465, 2222
BmgT120I	GGNCC	5 cut(s)	295, 1094, 1237, 1465, 2222
BmiI	GGNNCC	5 cut(s)	481, 561, 1227, 1953, 2223
BmrFI	CCNGG	2 cut(s)	299, 426
BmsI	GCATC	4 cut(s)	655, 1233, 1328, 1546
BoxI	GACNNNNGTC	1 cut(s)	182
BpmI	CTGGAG	4 cut(s)	917, 1071, 1662, 1970
Bpu10I	CCTNAGC	1 cut(s)	1380
BpuMI	CCSGG	1 cut(s)	299
Bsa29I	ATCGAT	1 cut(s)	2126
BsaHI	GRCGYC	2 cut(s)	65, 312
BsaI	GGTCTC	1 cut(s)	704
BsaJI	CCNNGG	6 cut(s)	100, 153, 298, 333, 424, 608
BsaWI	WCCGGW	2 cut(s)	269, 1239
Bsc4I	CCNNNNNNNGG	3 cut(s)	927, 1054, 1867
Bse118I	RCCGGY	2 cut(s)	131, 1939
Bse1I	ACTGG	6 cut(s)	1213, 1385, 1409, 1675, 1820, 1953
Bse3DI	GCAATG	2 cut(s)	659, 921
BseAI	TCCGGA	1 cut(s)	1239
BseBI	CCWGG	1 cut(s)	426
BseCI	ATCGAT	1 cut(s)	2126
BseDI	CCNNGG	6 cut(s)	100, 153, 298, 333, 424, 608
BseGI	GGATG	5 cut(s)	483, 1063, 1248, 1816, 2065
BseLI	CCNNNNNNNGG	3 cut(s)	927, 1054, 1867
BseMI	GCAATG	2 cut(s)	659, 921
BseMII	CTCAG	1 cut(s)	1371
BseNI	ACTGG	6 cut(s)	1213, 1385, 1409, 1675, 1820, 1953
BsePI	GCGCGC	2 cut(s)	146, 148
BseRI	GAGGAG	5 cut(s)	26, 76, 79, 86, 89
BseXI	GCAGC	5 cut(s)	640, 643, 1494, 1802, 1885
BseYI	CCCAGC	2 cut(s)	1283, 1762
BsgI	GTGCAG	2 cut(s)	734, 2213
Bsh1236I	CGCG	6 cut(s)	7, 16, 146, 148, 150, 306
Bsh1285I	CGRYCG	1 cut(s)	2126
BshFI	GGCC	2 cut(s)	1431, 1815
BshVI	ATCGAT	1 cut(s)	2126
BsiEI	CGRYCG	1 cut(s)	2126
BsiSI	CCGG	7 cut(s)	132, 231, 270, 298, 643, 1240, 1940
BslFI	GGGAC	6 cut(s)	55, 315, 1361, 1749, 2069, 2235
BslI	CCNNNNNNNGG	3 cut(s)	927, 1054, 1867
BsmAI	GTCTC	2 cut(s)	704, 1586
BsmFI	GGGAC	6 cut(s)	55, 315, 1361, 1749, 2069, 2235
BsmI	GAATGC	3 cut(s)	1246, 1533, 2170
BsnI	GGCC	2 cut(s)	1431, 1815
Bso31I	GGTCTC	1 cut(s)	704
Bsp1286I	GDGCHC	1 cut(s)	1805
Bsp13I	TCCGGA	1 cut(s)	1239
Bsp1407I	TGTACA	2 cut(s)	511, 976
Bsp19I	CCATGG	2 cut(s)	333, 608
BspACI	CCGC	8 cut(s)	5, 14, 118, 129, 199, 346, 374, 747
BspANI	GGCC	2 cut(s)	1431, 1815
BspCNI	CTCAG	1 cut(s)	1372
BspDI	ATCGAT	1 cut(s)	2126
BspEI	TCCGGA	1 cut(s)	1239
BspFNI	CGCG	6 cut(s)	7, 16, 146, 148, 150, 306
BspHI	TCATGA	1 cut(s)	2185
BspLI	GGNNCC	5 cut(s)	481, 561, 1227, 1953, 2223
BspMAI	CTGCAG	1 cut(s)	1696
BspPI	GGATC	4 cut(s)	271, 599, 1436, 1705
BspTI	CTTAAG	1 cut(s)	1220
BspTNI	GGTCTC	1 cut(s)	704
BsrBI	CCGCTC	1 cut(s)	346
BsrDI	GCAATG	2 cut(s)	659, 921
BsrFI	RCCGGY	2 cut(s)	131, 1939
BsrGI	TGTACA	2 cut(s)	511, 976
BsrI	ACTGG	6 cut(s)	1213, 1385, 1409, 1675, 1820, 1953
BssAI	RCCGGY	2 cut(s)	131, 1939
BssECI	CCNNGG	6 cut(s)	100, 153, 298, 333, 424, 608
BssHII	GCGCGC	2 cut(s)	146, 148
BssNI	GRCGYC	2 cut(s)	65, 312
BssSI	CACGAG	3 cut(s)	281, 290, 1575
BssT1I	CCWWGG	3 cut(s)	153, 333, 608
Bst2BI	CACGAG	3 cut(s)	281, 290, 1575
Bst2UI	CCWGG	1 cut(s)	426
Bst4CI	ACNGT	8 cut(s)	386, 532, 1469, 1564, 1597, 1727, 1930, 2002
Bst6I	CTCTTC	2 cut(s)	1056, 2124
BstACI	GRCGYC	2 cut(s)	65, 312
BstAFI	CTTAAG	1 cut(s)	1220
BstAPI	GCANNNNNTGC	1 cut(s)	1907
BstAUI	TGTACA	2 cut(s)	511, 976
BstC8I	GCNNGC	6 cut(s)	133, 148, 150, 1281, 1692, 2196
BstDEI	CTNAG	3 cut(s)	1053, 1154, 1380
BstDSI	CCRYGG	2 cut(s)	333, 608
BstF5I	GGATG	5 cut(s)	483, 1063, 1248, 1816, 2065
BstFNI	CGCG	6 cut(s)	7, 16, 146, 148, 150, 306
BstH2I	RGCGCY	1 cut(s)	321
BstHHI	GCGC	9 cut(s)	9, 18, 148, 150, 152, 220, 320, 1279, 2200
BstMAI	GTCTC	2 cut(s)	704, 1586
BstMCI	CGRYCG	1 cut(s)	2126
BstMWI	GCNNNNNNNGC	7 cut(s)	13, 15, 147, 923, 1285, 1862, 1907
BstNI	CCWGG	1 cut(s)	426
BstNSI	RCATGY	1 cut(s)	1283
BstPAI	GACNNNNGTC	1 cut(s)	182
BstSCI	CCNGG	2 cut(s)	297, 424
BstSFI	CTRYAG	2 cut(s)	1692, 1983
BstUI	CGCG	6 cut(s)	7, 16, 146, 148, 150, 306
BstV1I	GCAGC	5 cut(s)	640, 643, 1494, 1802, 1885
BstX2I	RGATCY	3 cut(s)	276, 604, 1441
BstXI	CCANNNNNNTGG	1 cut(s)	340
BstYI	RGATCY	3 cut(s)	276, 604, 1441
Bsu15I	ATCGAT	1 cut(s)	2126
BsuI	GTATCC	1 cut(s)	841
BsuRI	GGCC	2 cut(s)	1431, 1815
BsuTUI	ATCGAT	1 cut(s)	2126
BtgI	CCRYGG	2 cut(s)	333, 608
BtsCI	GGATG	5 cut(s)	483, 1063, 1248, 1816, 2065
BtsI	GCAGTG	2 cut(s)	961, 1685
BtsIMutI	CAGTG	5 cut(s)	961, 1206, 1392, 1685, 2232
Cac8I	GCNNGC	6 cut(s)	133, 148, 150, 1281, 1692, 2196
CciI	TCATGA	1 cut(s)	2185
CfoI	GCGC	9 cut(s)	9, 18, 148, 150, 152, 220, 320, 1279, 2200
Cfr10I	RCCGGY	2 cut(s)	131, 1939
Cfr13I	GGNCC	5 cut(s)	295, 1094, 1237, 1465, 2222
ClaI	ATCGAT	1 cut(s)	2126
CseI	GACGC	3 cut(s)	73, 301, 312
Csp6I	GTAC	7 cut(s)	387, 512, 977, 998, 1979, 1998, 2161
CviAII	CATG	9 cut(s)	187, 334, 609, 650, 949, 1280, 1360, 1862, 2186
CviQI	GTAC	7 cut(s)	387, 512, 977, 998, 1979, 1998, 2161
DdeI	CTNAG	3 cut(s)	1053, 1154, 1380
DraI	TTTAAA	2 cut(s)	964, 1117
EaeI	YGGCCR	1 cut(s)	1813
Eam1104I	CTCTTC	2 cut(s)	1056, 2124
EarI	CTCTTC	2 cut(s)	1056, 2124
EciI	GGCGGA	2 cut(s)	107, 118
Eco130I	CCWWGG	3 cut(s)	153, 333, 608
Eco24I	GRGCYC	1 cut(s)	1805
Eco31I	GGTCTC	1 cut(s)	704
Eco47I	GGWCC	5 cut(s)	295, 1094, 1237, 1465, 2222
Eco57I	CTGAAG	3 cut(s)	876, 1044, 1570
EcoO109I	RGGNCCY	1 cut(s)	2222
EcoRI	GAATTC	1 cut(s)	2147
EcoRII	CCWGG	1 cut(s)	424
EcoT14I	CCWWGG	3 cut(s)	153, 333, 608
EcoT22I	ATGCAT	1 cut(s)	1248
EcoT38I	GRGCYC	1 cut(s)	1805
ErhI	CCWWGG	3 cut(s)	153, 333, 608
FaeI	CATG	9 cut(s)	190, 337, 612, 653, 952, 1283, 1363, 1865, 2189
FalI	AAGNNNNNCTT	2 cut(s)	849, 881
FaqI	GGGAC	6 cut(s)	55, 315, 1361, 1749, 2069, 2235
FatI	CATG	9 cut(s)	186, 333, 608, 649, 948, 1279, 1359, 1861, 2185
FauI	CCCGC	2 cut(s)	21, 339
Fnu4HI	GCNGC	7 cut(s)	5, 375, 654, 657, 1508, 1791, 1899
FokI	GGATG	5 cut(s)	470, 1070, 1255, 1803, 2072
FriOI	GRGCYC	1 cut(s)	1805
Fsp4HI	GCNGC	7 cut(s)	5, 375, 654, 657, 1508, 1791, 1899
FspBI	CTAG	2 cut(s)	744, 1875
GlaI	GCGC	9 cut(s)	8, 17, 147, 149, 151, 219, 319, 1278, 2199
GluI	GCNGC	7 cut(s)	5, 375, 654, 657, 1508, 1791, 1899
GsaI	CCCAGC	2 cut(s)	1287, 1766
GsuI	CTGGAG	4 cut(s)	917, 1071, 1662, 1970
HaeII	RGCGCY	1 cut(s)	321
HaeIII	GGCC	2 cut(s)	1431, 1815
HapII	CCGG	7 cut(s)	132, 231, 270, 298, 643, 1240, 1940
HgaI	GACGC	3 cut(s)	73, 301, 312
HhaI	GCGC	9 cut(s)	9, 18, 148, 150, 152, 220, 320, 1279, 2200
Hin1I	GRCGYC	2 cut(s)	65, 312
Hin1II	CATG	9 cut(s)	190, 337, 612, 653, 952, 1283, 1363, 1865, 2189
Hin6I	GCGC	9 cut(s)	7, 16, 146, 148, 150, 218, 318, 1277, 2198
HinP1I	GCGC	9 cut(s)	7, 16, 146, 148, 150, 218, 318, 1277, 2198
HincII	GTYRAC	1 cut(s)	1146
HindII	GTYRAC	1 cut(s)	1146
HindIII	AAGCTT	1 cut(s)	1217
HpaI	GTTAAC	1 cut(s)	1146
HpaII	CCGG	7 cut(s)	132, 231, 270, 298, 643, 1240, 1940
HphI	GGTGA	6 cut(s)	238, 433, 1196, 1954, 2021, 2156
Hpy166II	GTNNAC	8 cut(s)	177, 512, 977, 1146, 1478, 1582, 1880, 2209
Hpy188I	TCNGA	9 cut(s)	209, 405, 627, 781, 835, 1252, 1454, 1539, 1589
Hpy8I	GTNNAC	8 cut(s)	177, 512, 977, 1146, 1478, 1582, 1880, 2209
Hpy99I	CGWCG	3 cut(s)	67, 311, 317
HpyCH4III	ACNGT	8 cut(s)	386, 532, 1469, 1564, 1597, 1727, 1930, 2002
HpyCH4IV	ACGT	3 cut(s)	168, 432, 1606
HpyF10VI	GCNNNNNNNGC	7 cut(s)	13, 15, 147, 923, 1285, 1862, 1907
HpyF3I	CTNAG	3 cut(s)	1053, 1154, 1380
HpySE526I	ACGT	3 cut(s)	168, 432, 1606
Hsp92I	GRCGYC	2 cut(s)	65, 312
Hsp92II	CATG	9 cut(s)	190, 337, 612, 653, 952, 1283, 1363, 1865, 2189
HspAI	GCGC	9 cut(s)	7, 16, 146, 148, 150, 218, 318, 1277, 2198
Kpn2I	TCCGGA	1 cut(s)	1239
KroI	GCCGGC	1 cut(s)	131
KroNI	GCCGGC	1 cut(s)	133
KspAI	GTTAAC	1 cut(s)	1146
LmnI	GCTCC	7 cut(s)	99, 201, 343, 479, 599, 645, 1951
Lsp1109I	GCAGC	5 cut(s)	640, 643, 1494, 1802, 1885
LweI	GCATC	4 cut(s)	655, 1233, 1328, 1546
MaeI	CTAG	2 cut(s)	744, 1875
MaeII	ACGT	3 cut(s)	168, 432, 1606
MaeIII	GTNAC	4 cut(s)	428, 1031, 1597, 1666
MauBI	CGCGCGCG	1 cut(s)	146
MbiI	CCGCTC	1 cut(s)	346
MboII	GAAGA	6 cut(s)	695, 779, 881, 1073, 1451, 2111
MflI	RGATCY	3 cut(s)	276, 604, 1441
MhlI	GDGCHC	1 cut(s)	1805
MlsI	TGGCCA	1 cut(s)	1815
MluNI	TGGCCA	1 cut(s)	1815
MlyI	GAGTC	5 cut(s)	221, 235, 323, 526, 2057
MmeI	TCCRAC	3 cut(s)	660, 1043, 1546
Mox20I	TGGCCA	1 cut(s)	1815
Mph1103I	ATGCAT	1 cut(s)	1248
MroI	TCCGGA	1 cut(s)	1239
MroNI	GCCGGC	1 cut(s)	131
MroXI	GAANNNNTTC	5 cut(s)	771, 861, 1533, 1778, 1830
MscI	TGGCCA	1 cut(s)	1815
MslI	CAYNNNNRTG	1 cut(s)	1251
Msp20I	TGGCCA	1 cut(s)	1815
MspCI	CTTAAG	1 cut(s)	1220
MspI	CCGG	7 cut(s)	132, 231, 270, 298, 643, 1240, 1940
MspR9I	CCNGG	2 cut(s)	299, 426
Mva1269I	GAATGC	3 cut(s)	1246, 1533, 2170
MvaI	CCWGG	1 cut(s)	426
MvnI	CGCG	6 cut(s)	7, 16, 146, 148, 150, 306
MwoI	GCNNNNNNNGC	7 cut(s)	13, 15, 147, 923, 1285, 1862, 1907
NaeI	GCCGGC	1 cut(s)	133
NciI	CCSGG	1 cut(s)	299
NcoI	CCATGG	2 cut(s)	333, 608
NgoMIV	GCCGGC	1 cut(s)	131
NlaIII	CATG	9 cut(s)	190, 337, 612, 653, 952, 1283, 1363, 1865, 2189
NlaIV	GGNNCC	5 cut(s)	481, 561, 1227, 1953, 2223
NmeAIII	GCCGAG	2 cut(s)	81, 194
NmuCI	GTSAC	1 cut(s)	428
NsiI	ATGCAT	1 cut(s)	1248
NspI	RCATGY	1 cut(s)	1283
PaeI	GCATGC	1 cut(s)	1283
PagI	TCATGA	1 cut(s)	2185
PauI	GCGCGC	2 cut(s)	146, 148
PcsI	WCGNNNNNNNCGW	2 cut(s)	165, 288
PctI	GAATGC	3 cut(s)	1246, 1533, 2170
PdiI	GCCGGC	1 cut(s)	133
PdmI	GAANNNNTTC	5 cut(s)	771, 861, 1533, 1778, 1830
PfeI	GAWTC	7 cut(s)	210, 256, 286, 622, 1158, 1421, 1871
PflFI	GACNNNGTC	3 cut(s)	67, 311, 1650
PflMI	CCANNNNNTGG	1 cut(s)	1867
PkrI	GCNGC	7 cut(s)	6, 376, 655, 658, 1509, 1792, 1900
Ple19I	CGATCG	1 cut(s)	2126
PleI	GAGTC	5 cut(s)	221, 235, 323, 525, 2057
PpsI	GAGTC	5 cut(s)	221, 235, 323, 525, 2057
PpuMI	RGGWCCY	1 cut(s)	2222
PshAI	GACNNNNGTC	1 cut(s)	182
PshBI	ATTAAT	1 cut(s)	1808
PsiI	TTATAA	1 cut(s)	578
Psp5II	RGGWCCY	1 cut(s)	2222
Psp6I	CCWGG	1 cut(s)	424
PspFI	CCCAGC	2 cut(s)	1283, 1762
PspGI	CCWGG	1 cut(s)	424
PspN4I	GGNNCC	5 cut(s)	481, 561, 1227, 1953, 2223
PspPI	GGNCC	5 cut(s)	295, 1094, 1237, 1465, 2222
PspPPI	RGGWCCY	1 cut(s)	2222
PstI	CTGCAG	1 cut(s)	1696
PsuI	RGATCY	3 cut(s)	276, 604, 1441
PsyI	GACNNNGTC	3 cut(s)	67, 311, 1650
PteI	GCGCGC	2 cut(s)	146, 148
PvuI	CGATCG	1 cut(s)	2126
RsaI	GTAC	7 cut(s)	388, 513, 978, 999, 1980, 1999, 2162
RsaNI	GTAC	7 cut(s)	387, 512, 977, 998, 1979, 1998, 2161
RseI	CAYNNNNRTG	1 cut(s)	1251
SatI	GCNGC	7 cut(s)	5, 375, 654, 657, 1508, 1791, 1899
Sau96I	GGNCC	5 cut(s)	295, 1094, 1237, 1465, 2222
SbfI	CCTGCAGG	1 cut(s)	1696
SchI	GAGTC	5 cut(s)	221, 235, 323, 526, 2057
ScrFI	CCNGG	2 cut(s)	299, 426
SdaI	CCTGCAGG	1 cut(s)	1696
SduI	GDGCHC	1 cut(s)	1805
SfaNI	GCATC	4 cut(s)	655, 1233, 1328, 1546
SfcI	CTRYAG	2 cut(s)	1692, 1983
SinI	GGWCC	5 cut(s)	295, 1094, 1237, 1465, 2222
SmiMI	CAYNNNNRTG	1 cut(s)	1251
SmlI	CTYRAG	1 cut(s)	1220
SmoI	CTYRAG	1 cut(s)	1220
SphI	GCATGC	1 cut(s)	1283
Sse8387I	CCTGCAGG	1 cut(s)	1696
SsiI	CCGC	8 cut(s)	5, 14, 118, 129, 199, 346, 374, 747
SspMI	CTAG	2 cut(s)	744, 1875
StyD4I	CCNGG	2 cut(s)	297, 424
StyI	CCWWGG	3 cut(s)	153, 333, 608
TaaI	ACNGT	8 cut(s)	386, 532, 1469, 1564, 1597, 1727, 1930, 2002
TaiI	ACGT	3 cut(s)	171, 435, 1609
TaqI	TCGA	6 cut(s)	225, 592, 635, 1680, 2122, 2126
TatI	WGTACW	4 cut(s)	386, 511, 976, 2160
TauI	GCSGC	2 cut(s)	7, 377
TfiI	GAWTC	7 cut(s)	210, 256, 286, 622, 1158, 1421, 1871
TscAI	CASTG	5 cut(s)	961, 1213, 1392, 1692, 2239
TseFI	GTSAC	1 cut(s)	428
TseI	GCWGC	5 cut(s)	653, 656, 1507, 1790, 1898
Tsp45I	GTSAC	1 cut(s)	428
TspDTI	ATGAA	6 cut(s)	560, 937, 1074, 1179, 1180, 1385
TspGWI	ACGGA	2 cut(s)	194, 1192
TspRI	CASTG	5 cut(s)	961, 1213, 1392, 1692, 2239
Tth111I	GACNNNGTC	3 cut(s)	67, 311, 1650
Van91I	CCANNNNNTGG	1 cut(s)	1867
Vha464I	CTTAAG	1 cut(s)	1220
VpaK11BI	GGWCC	5 cut(s)	295, 1094, 1237, 1465, 2222
VspI	ATTAAT	1 cut(s)	1808
XapI	RAATTY	5 cut(s)	932, 1715, 1753, 1988, 2147
XceI	RCATGY	1 cut(s)	1283
XmnI	GAANNNNTTC	5 cut(s)	771, 861, 1533, 1778, 1830
XspI	CTAG	2 cut(s)	744, 1875
Zsp2I	ATGCAT	1 cut(s)	1248

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.