MD07G1111000.v1.1

Mitochondrial GTPase that catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A- site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0012306
Physical Location & Seq
Forward (+)
12767673 .. 12774556
6884 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1111000.v1.1.491

Sequence Viewer

Length: 2277 bp
ATGACGCGCTTCCCTCCACCCTCCACGCCACGCCTCCTTTACACCCTCTACAGATCTAGAACGGCGCCGTCTCCTCCTTCCCCGTCCCCATCGGCAGCTTCTTCCCTCCTCATCGGCTCCTTCCACTTCCGTCAATTCTCCTCCGGCAACCTCGCGCGCGCCAAGGAGGACAAGGAGCCATGGTGGAAGGACTCCATGGACAAGCTTCGGAACATCGGAATCTCGGCCCACATCGACTCCGGCAAGACCACTCTCACCGAGAGAGTTCTGTTTTACACCGGGAAGATCCACGAGATTCACGAGGTTCGAGGCAGAGACGGCGTGGGTGCGAAAATGGATTCCATGGATTTGGAGAGGGAGAAGGGGATCACCATTCAGTCTGCTGCCACTTACTGTACTTGGAATGGCTATCAGATTAACATAATTGACACCCCAGGTCACGTTGATTTCACCATCGAGGTTGAGAGAGCTCTGCGTGTCCTTGATGGTGCCATTCTTGTCCTTTGTAGTGTTGGTGGAGTGCAAAGTCAGTCAATTACTGTTGATCGGCAAATGAAAAGATACGAGGTTCCAAGAATTGCATTTATAAACAAACTTGACCGAATGGGAGCAGATCCCTGGAAAGTTCTGAATCAGGCACGGGCTAAGCTCCGCCATCACAGTGCTGCCATGCAAGTTCCAATTGGGTTGGAAGAAGATTTTAAGGGTCTTATTGACCTTGTGCAGATGAAAGCTTACTATTTTCATGGTCCCAATGGTGAAAACATTGTTACTGAAGAAGTTCCTGCTGATATGGAGGAGTTCGTCACAGAAAAGAGGCGTGAGCTAATTGAAGTTGTATCTGAAGTTGATGACAAACTAGCTGAAGCATTCCTTGATGATGAGCCCATATCACCTACTGATCTTGAGGAAGCTGTGCGGAGGGCTACTGTAGCAAGGAAATTTATCCCCGTGTTCATGGGTAGTGCATTTAAAAACAAGGGTGTACAGCCACTTTTGAATGCTGTTCTTAATTATTTGCCTTGTCCGACTGAAGTCAGTAACTATGCTCTTGACCAGACTAAGGATGAAGAGAAGGTTACATTGGGTGGAACTCCGGATGGGCCTCTTGTAGCATTGGCTTTCAAATTGGAGGAAGGGCGTTTTGGTCAGTTAACATATCTAAGAATCTATGAAGGTGTCATTCGGAAGGGTGATTTTATTTTTAACATTAATACCGGTAAAAGGATTAAGGTTCCTCGTTTGGTTCGGATGCATTCCAATGAGATGGAGGATATTCAAGAGGCACATGCTGGACAAATCGTTGCTGTTTTTGGCGTGGACTGTGCATCAGGAGATACCTTTACTGATGGGTTGGTTAAATATACAATGACTTCTATGAGTGTCCCTGAGCCAGTGATGTCGTTAGCTGTTCAACCAGTTTCAAAAGATTCTGGAGGACAATTTTCAAAGGCTTTGAATCGTTTTCAGAAAGAGGATCCAACATTTCGTGTTGGCTTGGATCCTGAGAGCGGACAGACAATCATCTCTGGGATGGGAGAGCTGCATTTGGACATTTATGTTGAACGCATTCGGAGAGAATATAAGGTTGATGCCACTGTTGGAAAGCCTCGTGTTAACTTCAGAGAGACTGTTACTCAGCGGGCTGAGTTTGATTATTTACATAAGAAGCAATCTGGAGGACAAGGTCAATATGGGCGGGTATGTGGGTATATTGAACCACTCCCTGCTGACTCACAGACCAAGTTTGAATTTGAAAACATTATTGTGGGACAAGCTATACCATCAAATTTTATTCCAGCTATTGAGAAGGGTTTCAAAGAAGCAGCCAATTCGGGTTCATTAATTGGACATCCAGTTGAGCATGTTCGTATTGTATTAACCGATGGCGCTTCCCATGCCGTGGACTCCAGTGAACTTGCCTTTAAATTAGCTGCAATATATGCATTTAGAAAGTGCTATACGGCGGCAAGACCAGTGATATTGGAGCCTGTTATGCTGGTGGAATTGAAAGTACCTATAGAGTTTCAGGGTACTGTTGCTGGCGATCTTAACAAGAGAAAAGGTATCATTATTGGAAATGACCAGGAAGGAGATGACTCTGTTATTACTGCCCAAGTCCCTCTGAACAACATGTTTGGGTATTCGACTTCTCTTCGGTCAATGACTCAGGGAAAAGGCGAATTCACAATGGAGTACAAAGAGCACTCGCCGGTTTCTAATGATGTGCAGGCGCAACTGGTCAAGAACTACAAGGGCACCACTGCTGCTGAATAG

Protein Analysis

759

Amino Acids

84.2

Weight (kDa)

5.92

Isoelectric Point (pI)

40.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 68 - 341 8.2e-61 Elongation factor Tu GTP binding domain
EF-G_D2 PF22042 382 - 450 5.9e-10 Elongation factor G domain 2
GTP_EFTU_D2 PF03144 383 - 449 2.3e-13 Elongation factor Tu domain 2
EFG_III PF14492 462 - 536 2e-29 Elongation Factor G, domain III
EFG_IV PF03764 537 - 657 7.7e-38 Elongation factor G, domain IV
EFG_C PF00679 661 - 746 3.4e-26 Elongation factor G C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012306)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G45332 AT1G45332 AT2G45030
fragaria_vesca FvH4_7g10500 FvH4_7g10500 FvH4_7g10500
malus_domestica MD07G1111000.v1.1
prunus_persica Prupe.2G134500_v2.0.a1
pyrus_communis pycom07g09580
rosa_chinensis RchiOBHm_Chr1g0348291
rosa_laevigata RLG00000028669
rosa_multiflora Rmu_sc0027364.1_g000002
rosa_roxburghii Rroxscaffold_4G00307040
rosa_rugosa Rorug01G0194100
rosa_samantha Rh1AG211300 Rh1BG177800 Rh1CG195700 Rh1DG207300
rosa_wichuraiana Rw1G017850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 587
AccB1I GGYRCC 3 cut(s) 64, 488, 2258
AccB7I CCANNNNNTGG 1 cut(s) 1903
AccBSI CCGCTC 1 cut(s) 1512
AccII CGCG 4 cut(s) 7, 155, 157, 159
AccIII TCCGGA 1 cut(s) 1096
AciI CCGC 6 cut(s) 652, 919, 1512, 1642, 1699, 1967
AclWI GGATC 7 cut(s) 280, 374, 608, 1472, 1485, 1496, 1509
AcsI RAATTY 4 cut(s) 941, 1751, 1789, 2183
AcuI CTGAAG 5 cut(s) 795, 864, 885, 1053, 1606
AcyI GRCGYC 1 cut(s) 65
AfaI GTAC 5 cut(s) 397, 987, 2016, 2035, 2198
AfiI CCNNNNNNNGG 4 cut(s) 1063, 1224, 1511, 1903
AflIII ACRYGT 1 cut(s) 2133
AgeI ACCGGT 1 cut(s) 1217
AjnI CCWGG 3 cut(s) 433, 617, 2085
AjuI GAANNNNNNNTTGG 4 cut(s) 1067, 1099, 1128, 1160
Alw21I GWGCWC 2 cut(s) 472, 2208
Alw26I GTCTC 3 cut(s) 75, 309, 1622
AlwI GGATC 7 cut(s) 280, 374, 608, 1472, 1485, 1496, 1509
Aor13HI TCCGGA 1 cut(s) 1096
AoxI GGCC 2 cut(s) 225, 1103
ApeKI GCWGC 7 cut(s) 95, 383, 665, 1543, 1826, 1934, 2267
ApoI RAATTY 4 cut(s) 941, 1751, 1789, 2183
AseI ATTAAT 2 cut(s) 1212, 1844
AsiGI ACCGGT 1 cut(s) 1217
Asp700I GAANNNNTTC 3 cut(s) 780, 1569, 1814
AspLEI GCGC 7 cut(s) 9, 67, 157, 159, 161, 1892, 2236
AspS9I GGNCC 3 cut(s) 226, 749, 1103
AsuC2I CCSGG 1 cut(s) 280
AsuHPI GGTGA 6 cut(s) 247, 361, 442, 770, 885, 1205
AvaII GGWCC 1 cut(s) 749
BaeGI GKGCMC 1 cut(s) 2261
BamHI GGATCC 2 cut(s) 1477, 1501
BanI GGYRCC 3 cut(s) 64, 488, 2258
BanII GRGCYC 2 cut(s) 472, 888
BauI CACGAG 3 cut(s) 290, 299, 1611
Bbv12I GWGCWC 2 cut(s) 472, 2208
BbvI GCAGC 7 cut(s) 107, 370, 652, 1530, 1838, 1921, 2254
BceAI ACGGC 5 cut(s) 52, 78, 334, 1886, 1980
BcgI CGANNNNNNTGC 2 cut(s) 1815, 1849
BciT130I CCWGG 3 cut(s) 435, 619, 2087
BcnI CCSGG 1 cut(s) 280
BcoDI GTCTC 3 cut(s) 75, 309, 1622
BfaI CTAG 2 cut(s) 57, 860
BfmI CTRYAG 3 cut(s) 49, 930, 2019
BfoI RGCGCY 2 cut(s) 68, 1893
BglII AGATCT 1 cut(s) 53
BisI GCNGC 8 cut(s) 96, 384, 666, 1544, 1827, 1935, 1968, 2268
BlpI GCTNAGC 1 cut(s) 645
BlsI GCNGC 8 cut(s) 97, 385, 667, 1545, 1828, 1936, 1969, 2269
Bme1390I CCNGG 4 cut(s) 280, 435, 619, 2087
Bme18I GGWCC 1 cut(s) 749
BmgT120I GGNCC 3 cut(s) 226, 749, 1103
BmrFI CCNGG 4 cut(s) 280, 435, 619, 2087
BmsI GCATC 3 cut(s) 1242, 1337, 1582
BoxI GACNNNNGTC 1 cut(s) 1034
BpmI CTGGAG 3 cut(s) 1455, 1698, 1894
Bpu10I CCTNAGC 1 cut(s) 1389
Bpu1102I GCTNAGC 1 cut(s) 645
BpuEI CTTGAG 1 cut(s) 926
BpuMI CCSGG 1 cut(s) 280
BsaHI GRCGYC 1 cut(s) 65
BsaJI CCNNGG 7 cut(s) 162, 179, 195, 342, 433, 617, 1902
BsaWI WCCGGW 2 cut(s) 1096, 1217
BsaXI ACNNNNNCTCC 4 cut(s) 167, 197, 221, 251
Bsc4I CCNNNNNNNGG 4 cut(s) 1063, 1224, 1511, 1903
Bse118I RCCGGY 2 cut(s) 1217, 2212
Bse1I ACTGG 6 cut(s) 1394, 1418, 1856, 1911, 1976, 2244
BseAI TCCGGA 1 cut(s) 1096
BseBI CCWGG 3 cut(s) 435, 619, 2087
BseDI CCNNGG 7 cut(s) 162, 179, 195, 342, 433, 617, 1902
BseGI GGATG 5 cut(s) 1072, 1105, 1257, 1539, 1852
BseLI CCNNNNNNNGG 4 cut(s) 1063, 1224, 1511, 1903
BseMII CTCAG 5 cut(s) 1380, 1497, 1638, 1652, 2183
BseNI ACTGG 6 cut(s) 1394, 1418, 1856, 1911, 1976, 2244
BsePI GCGCGC 2 cut(s) 155, 157
BseRI GAGGAG 4 cut(s) 63, 98, 130, 812
BseSI GKGCMC 1 cut(s) 2261
BseXI GCAGC 7 cut(s) 107, 370, 652, 1530, 1838, 1921, 2254
BsgI GTGCAG 2 cut(s) 743, 2249
Bsh1236I CGCG 4 cut(s) 7, 155, 157, 159
BshFI GGCC 2 cut(s) 227, 1105
BshNI GGYRCC 3 cut(s) 64, 488, 2258
BshTI ACCGGT 1 cut(s) 1217
BsiHKAI GWGCWC 2 cut(s) 472, 2208
BsiSI CCGG 6 cut(s) 144, 240, 279, 1097, 1218, 2213
BslFI GGGAC 5 cut(s) 70, 735, 1370, 1785, 2105
BslI CCNNNNNNNGG 4 cut(s) 1063, 1224, 1511, 1903
BsmAI GTCTC 3 cut(s) 75, 309, 1622
BsmBI CGTCTC 2 cut(s) 75, 309
BsmFI GGGAC 5 cut(s) 70, 735, 1370, 1785, 2105
BsmI GAATGC 4 cut(s) 869, 1006, 1255, 1569
BsnI GGCC 2 cut(s) 227, 1105
Bsp1286I GDGCHC 4 cut(s) 472, 888, 2208, 2261
Bsp13I TCCGGA 1 cut(s) 1096
Bsp1407I TGTACA 1 cut(s) 985
Bsp143I GATC 9 cut(s) 53, 285, 366, 544, 613, 901, 1477, 1501, 2047
Bsp1720I GCTNAGC 1 cut(s) 645
Bsp19I CCATGG 3 cut(s) 179, 195, 342
BspACI CCGC 6 cut(s) 652, 919, 1512, 1642, 1699, 1967
BspANI GGCC 2 cut(s) 227, 1105
BspCNI CTCAG 5 cut(s) 1381, 1498, 1639, 1651, 2182
BspEI TCCGGA 1 cut(s) 1096
BspFNI CGCG 4 cut(s) 7, 155, 157, 159
BspPI GGATC 7 cut(s) 280, 374, 608, 1472, 1485, 1496, 1509
BspT107I GGYRCC 3 cut(s) 64, 488, 2258
BsrBI CCGCTC 1 cut(s) 1512
BsrFI RCCGGY 2 cut(s) 1217, 2212
BsrGI TGTACA 1 cut(s) 985
BsrI ACTGG 6 cut(s) 1394, 1418, 1856, 1911, 1976, 2244
BssAI RCCGGY 2 cut(s) 1217, 2212
BssECI CCNNGG 7 cut(s) 162, 179, 195, 342, 433, 617, 1902
BssHII GCGCGC 2 cut(s) 155, 157
BssMI GATC 9 cut(s) 53, 285, 366, 544, 613, 901, 1477, 1501, 2047
BssNI GRCGYC 1 cut(s) 65
BssSI CACGAG 3 cut(s) 290, 299, 1611
BssT1I CCWWGG 4 cut(s) 162, 179, 195, 342
Bst2BI CACGAG 3 cut(s) 290, 299, 1611
Bst2UI CCWGG 3 cut(s) 435, 619, 2087
Bst4CI ACNGT 8 cut(s) 395, 541, 662, 931, 1325, 1600, 1633, 2038
Bst6I CTCTTC 2 cut(s) 1065, 2160
BstACI GRCGYC 1 cut(s) 65
BstAPI GCANNNNNTGC 1 cut(s) 1943
BstAUI TGTACA 1 cut(s) 985
BstC8I GCNNGC 5 cut(s) 157, 159, 1644, 2044, 2232
BstDEI CTNAG 8 cut(s) 645, 1062, 1163, 1389, 1506, 1638, 1647, 2169
BstDSI CCRYGG 4 cut(s) 179, 195, 342, 1902
BstF5I GGATG 5 cut(s) 1072, 1105, 1257, 1539, 1852
BstFNI CGCG 4 cut(s) 7, 155, 157, 159
BstH2I RGCGCY 2 cut(s) 68, 1893
BstHHI GCGC 7 cut(s) 9, 67, 157, 159, 161, 1892, 2236
BstKTI GATC 9 cut(s) 56, 288, 369, 547, 616, 904, 1480, 1504, 2050
BstMAI GTCTC 3 cut(s) 75, 309, 1622
BstMBI GATC 9 cut(s) 53, 285, 366, 544, 613, 901, 1477, 1501, 2047
BstMWI GCNNNNNNNGC 5 cut(s) 318, 932, 1898, 1943, 1996
BstNI CCWGG 3 cut(s) 435, 619, 2087
BstNSI RCATGY 3 cut(s) 1292, 1868, 2137
BstPAI GACNNNNGTC 1 cut(s) 1034
BstSCI CCNGG 4 cut(s) 278, 433, 617, 2085
BstSFI CTRYAG 3 cut(s) 49, 930, 2019
BstSLI GKGCMC 1 cut(s) 2261
BstUI CGCG 4 cut(s) 7, 155, 157, 159
BstV1I GCAGC 7 cut(s) 107, 370, 652, 1530, 1838, 1921, 2254
BstX2I RGATCY 5 cut(s) 53, 285, 613, 1477, 1501
BstXI CCANNNNNNTGG 2 cut(s) 349, 1267
BstYI RGATCY 5 cut(s) 53, 285, 613, 1477, 1501
BsuRI GGCC 2 cut(s) 227, 1105
BtgI CCRYGG 4 cut(s) 179, 195, 342, 1902
BtsCI GGATG 5 cut(s) 1072, 1105, 1257, 1539, 1852
BtsI GCAGTG 1 cut(s) 2262
BtsIMutI CAGTG 6 cut(s) 667, 1401, 1596, 1918, 1983, 2262
Cac8I GCNNGC 5 cut(s) 157, 159, 1644, 2044, 2232
CfoI GCGC 7 cut(s) 9, 67, 157, 159, 161, 1892, 2236
Cfr10I RCCGGY 2 cut(s) 1217, 2212
Cfr13I GGNCC 3 cut(s) 226, 749, 1103
CseI GACGC 1 cut(s) 13
Csp6I GTAC 5 cut(s) 396, 986, 2015, 2034, 2197
CspAI ACCGGT 1 cut(s) 1217
CviQI GTAC 5 cut(s) 396, 986, 2015, 2034, 2197
DdeI CTNAG 8 cut(s) 645, 1062, 1163, 1389, 1506, 1638, 1647, 2169
DinI GGCGCC 1 cut(s) 66
DpnI GATC 9 cut(s) 55, 287, 368, 546, 615, 903, 1479, 1503, 2049
DpnII GATC 9 cut(s) 53, 285, 366, 544, 613, 901, 1477, 1501, 2047
DraI TTTAAA 2 cut(s) 973, 1927
Eam1104I CTCTTC 2 cut(s) 1065, 2160
EarI CTCTTC 2 cut(s) 1065, 2160
EciI GGCGGA 1 cut(s) 641
Ecl136II GAGCTC 1 cut(s) 470
Eco130I CCWWGG 4 cut(s) 162, 179, 195, 342
Eco24I GRGCYC 2 cut(s) 472, 888
Eco47I GGWCC 1 cut(s) 749
Eco53kI GAGCTC 1 cut(s) 470
Eco57I CTGAAG 5 cut(s) 795, 864, 885, 1053, 1606
EcoICRI GAGCTC 1 cut(s) 470
EcoRI GAATTC 1 cut(s) 2183
EcoRII CCWGG 3 cut(s) 433, 617, 2085
EcoT14I CCWWGG 4 cut(s) 162, 179, 195, 342
EcoT22I ATGCAT 2 cut(s) 1257, 1948
EcoT38I GRGCYC 2 cut(s) 472, 888
EgeI GGCGCC 1 cut(s) 66
EheI GGCGCC 1 cut(s) 66
ErhI CCWWGG 4 cut(s) 162, 179, 195, 342
Esp3I CGTCTC 2 cut(s) 75, 309
FalI AAGNNNNNCTT 2 cut(s) 858, 890
FaqI GGGAC 5 cut(s) 70, 735, 1370, 1785, 2105
FauI CCCGC 2 cut(s) 1635, 1692
Fnu4HI GCNGC 8 cut(s) 96, 384, 666, 1544, 1827, 1935, 1968, 2268
FokI GGATG 5 cut(s) 1079, 1112, 1264, 1546, 1839
FriOI GRGCYC 2 cut(s) 472, 888
Fsp4HI GCNGC 8 cut(s) 96, 384, 666, 1544, 1827, 1935, 1968, 2268
FspBI CTAG 2 cut(s) 57, 860
GlaI GCGC 7 cut(s) 8, 66, 156, 158, 160, 1891, 2235
GluI GCNGC 8 cut(s) 96, 384, 666, 1544, 1827, 1935, 1968, 2268
GsuI CTGGAG 3 cut(s) 1455, 1698, 1894
HaeII RGCGCY 2 cut(s) 68, 1893
HaeIII GGCC 2 cut(s) 227, 1105
HapII CCGG 6 cut(s) 144, 240, 279, 1097, 1218, 2213
HgaI GACGC 1 cut(s) 13
HhaI GCGC 7 cut(s) 9, 67, 157, 159, 161, 1892, 2236
Hin1I GRCGYC 1 cut(s) 65
Hin6I GCGC 7 cut(s) 7, 65, 155, 157, 159, 1890, 2234
HinP1I GCGC 7 cut(s) 7, 65, 155, 157, 159, 1890, 2234
HincII GTYRAC 2 cut(s) 1155, 1618
HindII GTYRAC 2 cut(s) 1155, 1618
HindIII AAGCTT 2 cut(s) 203, 732
HpaI GTTAAC 2 cut(s) 1155, 1618
HpaII CCGG 6 cut(s) 144, 240, 279, 1097, 1218, 2213
HphI GGTGA 6 cut(s) 247, 361, 442, 770, 885, 1205
Hpy166II GTNNAC 6 cut(s) 986, 1155, 1321, 1618, 1906, 1916
Hpy8I GTNNAC 6 cut(s) 986, 1155, 1321, 1618, 1906, 1916
HpyCH4III ACNGT 8 cut(s) 395, 541, 662, 931, 1325, 1600, 1633, 2038
HpyCH4IV ACGT 1 cut(s) 441
HpyF10VI GCNNNNNNNGC 5 cut(s) 318, 932, 1898, 1943, 1996
HpyF3I CTNAG 8 cut(s) 645, 1062, 1163, 1389, 1506, 1638, 1647, 2169
HpySE526I ACGT 1 cut(s) 441
Hsp92I GRCGYC 1 cut(s) 65
HspAI GCGC 7 cut(s) 7, 65, 155, 157, 159, 1890, 2234
KasI GGCGCC 1 cut(s) 64
Kpn2I TCCGGA 1 cut(s) 1096
KspAI GTTAAC 2 cut(s) 1155, 1618
Kzo9I GATC 9 cut(s) 53, 285, 366, 544, 613, 901, 1477, 1501, 2047
LmnI GCTCC 5 cut(s) 122, 175, 608, 654, 1987
Lsp1109I GCAGC 7 cut(s) 107, 370, 652, 1530, 1838, 1921, 2254
LweI GCATC 3 cut(s) 1242, 1337, 1582
MaeI CTAG 2 cut(s) 57, 860
MaeII ACGT 1 cut(s) 441
MaeIII GTNAC 6 cut(s) 437, 769, 805, 1040, 1078, 1633
MalI GATC 9 cut(s) 55, 287, 368, 546, 615, 903, 1479, 1503, 2049
MauBI CGCGCGCG 1 cut(s) 155
MbiI CCGCTC 1 cut(s) 1512
MboI GATC 9 cut(s) 53, 285, 366, 544, 613, 901, 1477, 1501, 2047
MboII GAAGA 7 cut(s) 93, 295, 704, 707, 788, 1082, 2147
MfeI CAATTG 1 cut(s) 681
MflI RGATCY 5 cut(s) 53, 285, 613, 1477, 1501
MhlI GDGCHC 4 cut(s) 472, 888, 2208, 2261
Mly113I GGCGCC 1 cut(s) 65
MlyI GAGTC 6 cut(s) 185, 230, 1727, 1901, 2093, 2161
MmeI TCCRAC 4 cut(s) 669, 1052, 1505, 1582
Mph1103I ATGCAT 2 cut(s) 1257, 1948
MroI TCCGGA 1 cut(s) 1096
MroXI GAANNNNTTC 3 cut(s) 780, 1569, 1814
MslI CAYNNNNRTG 3 cut(s) 660, 1260, 1766
MspA1I CMGCKG 1 cut(s) 1642
MspI CCGG 6 cut(s) 144, 240, 279, 1097, 1218, 2213
MspR9I CCNGG 4 cut(s) 280, 435, 619, 2087
MunI CAATTG 1 cut(s) 681
Mva1269I GAATGC 4 cut(s) 869, 1006, 1255, 1569
MvaI CCWGG 3 cut(s) 435, 619, 2087
MvnI CGCG 4 cut(s) 7, 155, 157, 159
MwoI GCNNNNNNNGC 5 cut(s) 318, 932, 1898, 1943, 1996
NarI GGCGCC 1 cut(s) 65
NciI CCSGG 1 cut(s) 280
NcoI CCATGG 3 cut(s) 179, 195, 342
NdeII GATC 9 cut(s) 53, 285, 366, 544, 613, 901, 1477, 1501, 2047
NmeAIII GCCGAG 1 cut(s) 203
NmuCI GTSAC 2 cut(s) 437, 805
NsiI ATGCAT 2 cut(s) 1257, 1948
NspI RCATGY 3 cut(s) 1292, 1868, 2137
PauI GCGCGC 2 cut(s) 155, 157
PciI ACATGT 1 cut(s) 2133
PcsI WCGNNNNNNNCGW 1 cut(s) 297
PctI GAATGC 4 cut(s) 869, 1006, 1255, 1569
PdmI GAANNNNTTC 3 cut(s) 780, 1569, 1814
PfeI GAWTC 7 cut(s) 219, 295, 338, 631, 1167, 1430, 1459
PflFI GACNNNGTC 1 cut(s) 1686
PflMI CCANNNNNTGG 1 cut(s) 1903
PinAI ACCGGT 1 cut(s) 1217
PkrI GCNGC 8 cut(s) 97, 385, 667, 1545, 1828, 1936, 1969, 2269
PleI GAGTC 6 cut(s) 185, 230, 1727, 1901, 2093, 2161
PluTI GGCGCC 1 cut(s) 68
PpsI GAGTC 6 cut(s) 185, 230, 1727, 1901, 2093, 2161
PscI ACATGT 1 cut(s) 2133
PshAI GACNNNNGTC 1 cut(s) 1034
PshBI ATTAAT 2 cut(s) 1212, 1844
PsiI TTATAA 1 cut(s) 587
Psp124BI GAGCTC 1 cut(s) 472
Psp6I CCWGG 3 cut(s) 433, 617, 2085
PspGI CCWGG 3 cut(s) 433, 617, 2085
PspPI GGNCC 3 cut(s) 226, 749, 1103
PsuI RGATCY 5 cut(s) 53, 285, 613, 1477, 1501
PsyI GACNNNGTC 1 cut(s) 1686
PteI GCGCGC 2 cut(s) 155, 157
RsaI GTAC 5 cut(s) 397, 987, 2016, 2035, 2198
RsaNI GTAC 5 cut(s) 396, 986, 2015, 2034, 2197
RseI CAYNNNNRTG 3 cut(s) 660, 1260, 1766
SacI GAGCTC 1 cut(s) 472
SatI GCNGC 8 cut(s) 96, 384, 666, 1544, 1827, 1935, 1968, 2268
Sau3AI GATC 9 cut(s) 53, 285, 366, 544, 613, 901, 1477, 1501, 2047
Sau96I GGNCC 3 cut(s) 226, 749, 1103
SchI GAGTC 6 cut(s) 185, 230, 1727, 1901, 2093, 2161
ScrFI CCNGG 4 cut(s) 280, 435, 619, 2087
SduI GDGCHC 4 cut(s) 472, 888, 2208, 2261
SfaNI GCATC 3 cut(s) 1242, 1337, 1582
SfcI CTRYAG 3 cut(s) 49, 930, 2019
SfoI GGCGCC 1 cut(s) 66
SinI GGWCC 1 cut(s) 749
SmiMI CAYNNNNRTG 3 cut(s) 660, 1260, 1766
SmlI CTYRAG 1 cut(s) 905
SmoI CTYRAG 1 cut(s) 905
SsiI CCGC 6 cut(s) 652, 919, 1512, 1642, 1699, 1967
SspDI GGCGCC 1 cut(s) 64
SspMI CTAG 2 cut(s) 57, 860
SstI GAGCTC 1 cut(s) 472
StyD4I CCNGG 4 cut(s) 278, 433, 617, 2085
StyI CCWWGG 4 cut(s) 162, 179, 195, 342
TaaI ACNGT 8 cut(s) 395, 541, 662, 931, 1325, 1600, 1633, 2038
TaiI ACGT 1 cut(s) 444
TaqI TCGA 4 cut(s) 234, 307, 456, 2147
TaqII GACCGA 2 cut(s) 615, 2148
TatI WGTACW 3 cut(s) 395, 985, 2196
TauI GCSGC 1 cut(s) 1970
TfiI GAWTC 7 cut(s) 219, 295, 338, 631, 1167, 1430, 1459
TscAI CASTG 6 cut(s) 667, 1401, 1603, 1918, 1983, 2269
TseFI GTSAC 2 cut(s) 437, 805
TseI GCWGC 7 cut(s) 95, 383, 665, 1543, 1826, 1934, 2267
Tsp45I GTSAC 2 cut(s) 437, 805
TspDTI ATGAA 7 cut(s) 569, 734, 743, 946, 1083, 1188, 1830
TspGWI ACGGA 1 cut(s) 119
TspRI CASTG 6 cut(s) 667, 1401, 1603, 1918, 1983, 2269
Tth111I GACNNNGTC 1 cut(s) 1686
Van91I CCANNNNNTGG 1 cut(s) 1903
VpaK11BI GGWCC 1 cut(s) 749
VspI ATTAAT 2 cut(s) 1212, 1844
XapI RAATTY 4 cut(s) 941, 1751, 1789, 2183
XbaI TCTAGA 1 cut(s) 56
XceI RCATGY 3 cut(s) 1292, 1868, 2137
XmnI GAANNNNTTC 3 cut(s) 780, 1569, 1814
XspI CTAG 2 cut(s) 57, 860
Zsp2I ATGCAT 2 cut(s) 1257, 1948
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.