Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr7

Orthogroup ID

OG0012306

Physical Location & Seq

Genomic Coordinates Forward (+)

8853352 .. 8859963

Gene Structure 6612 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom07g09580.5

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2208 bp

ATGACGCGCTTCCCTCCACCCTCCACGCCACGCCTCCTTTACACCCTCTACAGATCTCGAACGGCGACGTCTCCTCCTTCCCCGTCCCCATCGGCAGCGTCTTCCCTCCTCATCGGCGCCTTCCACTTCCGCCAATTCTCCTCCGGCAACCTCGCGCGCGCCAAGGAGGACAAGGAGCCATGGTGGAAGGACTCCATGGACAAGCTTCGGAACATCGGAATCTCGGCCCACATCGACTCCGGCAAGACCACCCTCACCGAGAGAGTTCTGTTTTACACCGGGAAGATCCACGAGATTCACGAGGTTCGAGGCAGAGACGGCGTGGGTGCGAAAATGGATTCCATGGATTTGGAGAGGGAAAAGGGGATCACCATTCAGTCTGCTGCCACTTACTGTACTTGGAATGGCTATCAGATTAACATAATTGACACCCCAGGTCACGTTGATTTCACCATCGAGGTTGAGAGAGCTCTGCGTGTCCTTGATGGTGCCATTCTTGTCCTTTGTAGTGTTGGTGGAGTGCAAAGTCAGTCAATTACTGTTGATCGGCAAATGAAAAGATATGAGGTTCCAAGAATTGCATTTATAAACAAACTTGACCGAATGGGAGCAGATCCCTGGAAAGTTCTGAACCAGGCACGGGCTAAGCTCCGCCATCACAGTGCTGCCATGCAAGTTCCAATTGGGTTGGAAGAAGATTTTAAGGGTCTTATTGACCTTGTGCAGATGAAAGCTTACTATTTTCATGGTCCCAATGGTGAAAACATTATTACTGAAGAAGTTCCTGCTGATATGGAGGAGTTCGTCACAGAAAAGAGGCGCGAGCTAATTGAAGTTGTATCTGAAGTTGATGACAAACTAGCTGAAGCATTCCTTGATGATGAGCCCATATCACCTACTGATCTTGAGGAAGCTGTGCGGAGGGCTACTGTAGCAAGGAAGTTTATCCCTGTGTTCATGGGTAGTGCATTTAAAAACAAGGGTGTACAGCCACTTTTGAATGCTGTTCTTAATTATTTGCCTTGTCCGACTGAAGTCAGTAACTATGCTCTTGACCAGACTAATGATGAAGAGAAGGTTACATTAGGTGGAACTCCGGATGGGCCTCTTGTAGCATTGGCTTTCAAATTGGAGGAAGGGCGTTTTGGTCAGTTAACATATCTAAGAATCTATGAAGGTGTCATTCGGAAGGGTGATTTTATTTTTAACATTAATACCGGTAAAAGGATTAAGGTTCCTCGCTTGGTTCGGATGCATTCCAATGAGATGGAGGATATTCAAGAGGCACATGCTGGGCAAATCGTTGCTGTTTTTGGAGTGGACTGTGCATCAGGAGATACCTTTACTGATGGGTCGGTTAAATATACAATGACTTCTATGAGTGTCCCCGAGCCAGTGATGTCATTAGCTGTTCAACCAGTTTCAAAAGATTCTGGAGGACAATTCTCAAAGGCTTTGAATCGTTTTCAGAAAGAGGATCCAACATTTCGTGTTGGCTTGGATCCTGAGAGCGGACAGGTTGATGCCACTGTTGGAAAACCTCGTGTTAACTTCAGAGAGACTGTTACTCAGCGGGCTGAGTTTGATTATTTACATAAGAAGCAATCTGGAGGACAAGGTCAATATGGGCGGGTATGTGGGTATATTGAACCACTCCCTGCTGACTCACAGACCAAGTTTGAATTTGAAAACATTATTGTGGGACAAGCTATACCATCAAATTTTATTCCAGCTATTGAGAAGGGTTTCAAAGAAGCAGCCAATTCGGGTTCATTAATTGGACATCCAGTTGAGCATGTTCGTATTGTATTAACTGATGGCGCTTCCCATGCCGTGGACTCCAGTGAACTTGCCTTTAAATTAGCTGCAATTTATGCATTTAGAAAGTGCTACACGGCAGCAAGACCAGTGATATTGGAGCCCGTTATGCTGGTGGAATTGAAAGTACCTATAGAGTTTCAGGGTACTGTTGCTGGTGATCTTAACAAGAGAAAAGGTATCATTATTGGAAATGACCAGGAAGGAGATGACTCTGTTATTACTGCCCAAGTACCTCTGAACAACATGTTTGGGTATTCGACTTCTCTTCGGTCAATGACTCAGGGAAAAGGCGAATTCACAATGGAGTACAAAGAGCACTCGCCGGTTTCTAATGATGTGCAGGCTCAACTAGTCAAGAACTACAAGGGCACCACTGCTGCTGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003676 GO:0003723 GO:0003746 GO:0003824 GO:0003924 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006412 GO:0006414 GO:0006518 GO:0006807 GO:0008135 GO:0008144 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019538 GO:0030554 GO:0032543 GO:0032553 GO:0032555 GO:0032559 GO:0034641 GO:0034645 GO:0035639 GO:0036094 GO:0042221 GO:0043043 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0050896 GO:0070125 GO:0071704 GO:0097159 GO:0097367 GO:0140053 GO:1901265 GO:1901363 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EFG_C EFG_II EFG_IV GTP_EFTU GTP_EFTU_D2

Protein Analysis

736

Amino Acids

81.39

Weight (kDa)

5.81

Isoelectric Point (pI)

40.68

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012306)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G45332 AT1G45332 AT2G45030
fragaria_vesca	FvH4_7g10500 FvH4_7g10500 FvH4_7g10500
malus_domestica	MD07G1111000.v1.1
prunus_persica	Prupe.2G134500_v2.0.a1
pyrus_communis	pycom07g09580
rosa_chinensis	RchiOBHm_Chr1g0348291
rosa_laevigata	RLG00000028669
rosa_multiflora	Rmu_sc0027364.1_g000002
rosa_roxburghii	Rroxscaffold_4G00307040
rosa_rugosa	Rorug01G0194100
rosa_samantha	Rh1AG211300 Rh1BG177800 Rh1CG195700 Rh1DG207300
rosa_wichuraiana	Rw1G017850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	587
AatII	GACGTC	1 cut(s)	71
AccB1I	GGYRCC	3 cut(s)	116, 488, 2189
AccB7I	CCANNNNNTGG	1 cut(s)	1834
AccBSI	CCGCTC	1 cut(s)	1512
AccII	CGCG	5 cut(s)	7, 155, 157, 159, 822
AccIII	TCCGGA	1 cut(s)	1096
AciI	CCGC	6 cut(s)	130, 652, 919, 1512, 1573, 1630
AclWI	GGATC	7 cut(s)	280, 374, 608, 1472, 1485, 1496, 1509
AcsI	RAATTY	3 cut(s)	1682, 1720, 2114
AcuI	CTGAAG	5 cut(s)	795, 864, 885, 1053, 1537
AcyI	GRCGYC	2 cut(s)	68, 117
AfaI	GTAC	6 cut(s)	397, 987, 1947, 1966, 2052, 2129
AfiI	CCNNNNNNNGG	4 cut(s)	640, 1224, 1511, 1834
AflIII	ACRYGT	1 cut(s)	2064
AgeI	ACCGGT	1 cut(s)	1217
AhlI	ACTAGT	1 cut(s)	2170
AjnI	CCWGG	4 cut(s)	433, 617, 633, 2016
AjuI	GAANNNNNNNTTGG	2 cut(s)	1128, 1160
Alw21I	GWGCWC	2 cut(s)	472, 2139
Alw26I	GTCTC	3 cut(s)	75, 309, 1553
AlwI	GGATC	7 cut(s)	280, 374, 608, 1472, 1485, 1496, 1509
Ama87I	CYCGRG	1 cut(s)	1388
Aor13HI	TCCGGA	1 cut(s)	1096
AoxI	GGCC	2 cut(s)	225, 1103
ApeKI	GCWGC	7 cut(s)	95, 383, 665, 1757, 1865, 1898, 2198
ApoI	RAATTY	3 cut(s)	1682, 1720, 2114
AseI	ATTAAT	2 cut(s)	1212, 1775
AsiGI	ACCGGT	1 cut(s)	1217
Asp700I	GAANNNNTTC	2 cut(s)	780, 1745
AspLEI	GCGC	7 cut(s)	9, 119, 157, 159, 161, 822, 1823
AspS9I	GGNCC	3 cut(s)	226, 749, 1103
AsuC2I	CCSGG	1 cut(s)	280
AsuHPI	GGTGA	7 cut(s)	247, 361, 442, 770, 885, 1205, 1988
AvaI	CYCGRG	1 cut(s)	1388
AvaII	GGWCC	1 cut(s)	749
BaeGI	GKGCMC	1 cut(s)	2192
BamHI	GGATCC	2 cut(s)	1477, 1501
BanI	GGYRCC	3 cut(s)	116, 488, 2189
BanII	GRGCYC	3 cut(s)	472, 888, 1923
BauI	CACGAG	3 cut(s)	290, 299, 1542
BbsI	GAAGAC	1 cut(s)	93
Bbv12I	GWGCWC	2 cut(s)	472, 2139
BbvI	GCAGC	7 cut(s)	107, 370, 652, 1769, 1852, 1910, 2185
BccI	CCATC	9 cut(s)	97, 461, 479, 663, 1094, 1261, 1343, 1723, 1811
BceAI	ACGGC	4 cut(s)	78, 334, 1817, 1911
BcgI	CGANNNNNNTGC	2 cut(s)	1746, 1780
BciT130I	CCWGG	4 cut(s)	435, 619, 635, 2018
BcnI	CCSGG	1 cut(s)	280
BcoDI	GTCTC	3 cut(s)	75, 309, 1553
BcuI	ACTAGT	1 cut(s)	2170
BfaI	CTAG	2 cut(s)	860, 2171
BfmI	CTRYAG	3 cut(s)	49, 930, 1950
BfoI	RGCGCY	2 cut(s)	120, 1824
BglII	AGATCT	1 cut(s)	53
BisI	GCNGC	7 cut(s)	96, 384, 666, 1758, 1866, 1899, 2199
BlpI	GCTNAGC	1 cut(s)	645
BlsI	GCNGC	7 cut(s)	97, 385, 667, 1759, 1867, 1900, 2200
Bme1390I	CCNGG	5 cut(s)	280, 435, 619, 635, 2018
Bme18I	GGWCC	1 cut(s)	749
BmeT110I	CYCGRG	1 cut(s)	1388
BmgT120I	GGNCC	3 cut(s)	226, 749, 1103
BmrFI	CCNGG	5 cut(s)	280, 435, 619, 635, 2018
BmsI	GCATC	3 cut(s)	1242, 1337, 1513
BoxI	GACNNNNGTC	1 cut(s)	1034
BpiI	GAAGAC	1 cut(s)	93
BpmI	CTGGAG	3 cut(s)	1455, 1629, 1825
Bpu1102I	GCTNAGC	1 cut(s)	645
BpuEI	CTTGAG	1 cut(s)	926
BpuMI	CCSGG	1 cut(s)	280
BsaHI	GRCGYC	2 cut(s)	68, 117
BsaJI	CCNNGG	7 cut(s)	162, 179, 195, 342, 433, 617, 1833
BsaWI	WCCGGW	2 cut(s)	1096, 1217
BsaXI	ACNNNNNCTCC	6 cut(s)	58, 88, 167, 197, 221, 251
Bsc4I	CCNNNNNNNGG	4 cut(s)	640, 1224, 1511, 1834
Bse118I	RCCGGY	2 cut(s)	1217, 2143
Bse1I	ACTGG	5 cut(s)	1394, 1418, 1787, 1842, 1907
BseAI	TCCGGA	1 cut(s)	1096
BseBI	CCWGG	4 cut(s)	435, 619, 635, 2018
BseDI	CCNNGG	7 cut(s)	162, 179, 195, 342, 433, 617, 1833
BseGI	GGATG	3 cut(s)	1105, 1257, 1783
BseLI	CCNNNNNNNGG	4 cut(s)	640, 1224, 1511, 1834
BseMII	CTCAG	4 cut(s)	1497, 1569, 1583, 2114
BseNI	ACTGG	5 cut(s)	1394, 1418, 1787, 1842, 1907
BsePI	GCGCGC	2 cut(s)	155, 157
BseRI	GAGGAG	4 cut(s)	63, 98, 130, 812
BseSI	GKGCMC	1 cut(s)	2192
BseXI	GCAGC	7 cut(s)	107, 370, 652, 1769, 1852, 1910, 2185
BseYI	CCCAGC	1 cut(s)	1292
BsgI	GTGCAG	2 cut(s)	743, 2180
Bsh1236I	CGCG	5 cut(s)	7, 155, 157, 159, 822
BshFI	GGCC	2 cut(s)	227, 1105
BshNI	GGYRCC	3 cut(s)	116, 488, 2189
BshTI	ACCGGT	1 cut(s)	1217
BsiHKAI	GWGCWC	2 cut(s)	472, 2139
BsiHKCI	CYCGRG	1 cut(s)	1388
BsiSI	CCGG	6 cut(s)	144, 240, 279, 1097, 1218, 2144
BslFI	GGGAC	4 cut(s)	70, 735, 1370, 1716
BslI	CCNNNNNNNGG	4 cut(s)	640, 1224, 1511, 1834
BsmAI	GTCTC	3 cut(s)	75, 309, 1553
BsmBI	CGTCTC	2 cut(s)	75, 309
BsmFI	GGGAC	4 cut(s)	70, 735, 1370, 1716
BsmI	GAATGC	3 cut(s)	869, 1006, 1255
BsnI	GGCC	2 cut(s)	227, 1105
BsoBI	CYCGRG	1 cut(s)	1388
Bsp1286I	GDGCHC	5 cut(s)	472, 888, 1923, 2139, 2192
Bsp13I	TCCGGA	1 cut(s)	1096
Bsp1407I	TGTACA	1 cut(s)	985
Bsp143I	GATC	9 cut(s)	53, 285, 366, 544, 613, 901, 1477, 1501, 1978
Bsp1720I	GCTNAGC	1 cut(s)	645
Bsp19I	CCATGG	3 cut(s)	179, 195, 342
BspACI	CCGC	6 cut(s)	130, 652, 919, 1512, 1573, 1630
BspANI	GGCC	2 cut(s)	227, 1105
BspCNI	CTCAG	4 cut(s)	1498, 1570, 1582, 2113
BspEI	TCCGGA	1 cut(s)	1096
BspFNI	CGCG	5 cut(s)	7, 155, 157, 159, 822
BspPI	GGATC	7 cut(s)	280, 374, 608, 1472, 1485, 1496, 1509
BspT107I	GGYRCC	3 cut(s)	116, 488, 2189
BsrBI	CCGCTC	1 cut(s)	1512
BsrFI	RCCGGY	2 cut(s)	1217, 2143
BsrGI	TGTACA	1 cut(s)	985
BsrI	ACTGG	5 cut(s)	1394, 1418, 1787, 1842, 1907
BssAI	RCCGGY	2 cut(s)	1217, 2143
BssECI	CCNNGG	7 cut(s)	162, 179, 195, 342, 433, 617, 1833
BssHII	GCGCGC	2 cut(s)	155, 157
BssMI	GATC	9 cut(s)	53, 285, 366, 544, 613, 901, 1477, 1501, 1978
BssNI	GRCGYC	2 cut(s)	68, 117
BssSI	CACGAG	3 cut(s)	290, 299, 1542
BssT1I	CCWWGG	4 cut(s)	162, 179, 195, 342
Bst2BI	CACGAG	3 cut(s)	290, 299, 1542
Bst2UI	CCWGG	4 cut(s)	435, 619, 635, 2018
Bst4CI	ACNGT	8 cut(s)	395, 541, 662, 931, 1325, 1531, 1564, 1969
Bst6I	CTCTTC	2 cut(s)	1065, 2091
BstACI	GRCGYC	2 cut(s)	68, 117
BstAPI	GCANNNNNTGC	1 cut(s)	1874
BstAUI	TGTACA	1 cut(s)	985
BstC8I	GCNNGC	5 cut(s)	157, 159, 824, 1575, 2163
BstDEI	CTNAG	6 cut(s)	645, 1163, 1506, 1569, 1578, 2100
BstDSI	CCRYGG	4 cut(s)	179, 195, 342, 1833
BstF5I	GGATG	3 cut(s)	1105, 1257, 1783
BstFNI	CGCG	5 cut(s)	7, 155, 157, 159, 822
BstH2I	RGCGCY	2 cut(s)	120, 1824
BstHHI	GCGC	7 cut(s)	9, 119, 157, 159, 161, 822, 1823
BstKTI	GATC	9 cut(s)	56, 288, 369, 547, 616, 904, 1480, 1504, 1981
BstMAI	GTCTC	3 cut(s)	75, 309, 1553
BstMBI	GATC	9 cut(s)	53, 285, 366, 544, 613, 901, 1477, 1501, 1978
BstMWI	GCNNNNNNNGC	5 cut(s)	318, 932, 1829, 1874, 1927
BstNI	CCWGG	4 cut(s)	435, 619, 635, 2018
BstNSI	RCATGY	3 cut(s)	1292, 1799, 2068
BstPAI	GACNNNNGTC	1 cut(s)	1034
BstSCI	CCNGG	5 cut(s)	278, 433, 617, 633, 2016
BstSFI	CTRYAG	3 cut(s)	49, 930, 1950
BstSLI	GKGCMC	1 cut(s)	2192
BstUI	CGCG	5 cut(s)	7, 155, 157, 159, 822
BstV1I	GCAGC	7 cut(s)	107, 370, 652, 1769, 1852, 1910, 2185
BstV2I	GAAGAC	1 cut(s)	93
BstX2I	RGATCY	5 cut(s)	53, 285, 613, 1477, 1501
BstXI	CCANNNNNNTGG	2 cut(s)	349, 1267
BstYI	RGATCY	5 cut(s)	53, 285, 613, 1477, 1501
BsuRI	GGCC	2 cut(s)	227, 1105
BtgI	CCRYGG	4 cut(s)	179, 195, 342, 1833
BtsCI	GGATG	3 cut(s)	1105, 1257, 1783
BtsI	GCAGTG	1 cut(s)	2193
BtsIMutI	CAGTG	6 cut(s)	667, 1401, 1527, 1849, 1914, 2193
Cac8I	GCNNGC	5 cut(s)	157, 159, 824, 1575, 2163
CfoI	GCGC	7 cut(s)	9, 119, 157, 159, 161, 822, 1823
Cfr10I	RCCGGY	2 cut(s)	1217, 2143
Cfr13I	GGNCC	3 cut(s)	226, 749, 1103
CseI	GACGC	2 cut(s)	13, 87
Csp6I	GTAC	6 cut(s)	396, 986, 1946, 1965, 2051, 2128
CspAI	ACCGGT	1 cut(s)	1217
CviQI	GTAC	6 cut(s)	396, 986, 1946, 1965, 2051, 2128
DdeI	CTNAG	6 cut(s)	645, 1163, 1506, 1569, 1578, 2100
DinI	GGCGCC	1 cut(s)	118
DpnI	GATC	9 cut(s)	55, 287, 368, 546, 615, 903, 1479, 1503, 1980
DpnII	GATC	9 cut(s)	53, 285, 366, 544, 613, 901, 1477, 1501, 1978
DraI	TTTAAA	2 cut(s)	973, 1858
Eam1104I	CTCTTC	2 cut(s)	1065, 2091
EarI	CTCTTC	2 cut(s)	1065, 2091
EciI	GGCGGA	2 cut(s)	119, 641
Ecl136II	GAGCTC	1 cut(s)	470
Eco130I	CCWWGG	4 cut(s)	162, 179, 195, 342
Eco24I	GRGCYC	3 cut(s)	472, 888, 1923
Eco47I	GGWCC	1 cut(s)	749
Eco53kI	GAGCTC	1 cut(s)	470
Eco57I	CTGAAG	5 cut(s)	795, 864, 885, 1053, 1537
Eco88I	CYCGRG	1 cut(s)	1388
EcoICRI	GAGCTC	1 cut(s)	470
EcoRI	GAATTC	1 cut(s)	2114
EcoRII	CCWGG	4 cut(s)	433, 617, 633, 2016
EcoT14I	CCWWGG	4 cut(s)	162, 179, 195, 342
EcoT22I	ATGCAT	2 cut(s)	1257, 1879
EcoT38I	GRGCYC	3 cut(s)	472, 888, 1923
EgeI	GGCGCC	1 cut(s)	118
EheI	GGCGCC	1 cut(s)	118
ErhI	CCWWGG	4 cut(s)	162, 179, 195, 342
Esp3I	CGTCTC	2 cut(s)	75, 309
FalI	AAGNNNNNCTT	2 cut(s)	858, 890
FaqI	GGGAC	4 cut(s)	70, 735, 1370, 1716
FauI	CCCGC	2 cut(s)	1566, 1623
Fnu4HI	GCNGC	7 cut(s)	96, 384, 666, 1758, 1866, 1899, 2199
FokI	GGATG	3 cut(s)	1112, 1264, 1770
FriOI	GRGCYC	3 cut(s)	472, 888, 1923
Fsp4HI	GCNGC	7 cut(s)	96, 384, 666, 1758, 1866, 1899, 2199
FspBI	CTAG	2 cut(s)	860, 2171
GlaI	GCGC	7 cut(s)	8, 118, 156, 158, 160, 821, 1822
GluI	GCNGC	7 cut(s)	96, 384, 666, 1758, 1866, 1899, 2199
GsaI	CCCAGC	1 cut(s)	1296
GsuI	CTGGAG	3 cut(s)	1455, 1629, 1825
HaeII	RGCGCY	2 cut(s)	120, 1824
HaeIII	GGCC	2 cut(s)	227, 1105
HapII	CCGG	6 cut(s)	144, 240, 279, 1097, 1218, 2144
HgaI	GACGC	2 cut(s)	13, 87
HhaI	GCGC	7 cut(s)	9, 119, 157, 159, 161, 822, 1823
Hin1I	GRCGYC	2 cut(s)	68, 117
Hin6I	GCGC	7 cut(s)	7, 117, 155, 157, 159, 820, 1821
HinP1I	GCGC	7 cut(s)	7, 117, 155, 157, 159, 820, 1821
HincII	GTYRAC	2 cut(s)	1155, 1549
HindII	GTYRAC	2 cut(s)	1155, 1549
HindIII	AAGCTT	2 cut(s)	203, 732
HpaI	GTTAAC	2 cut(s)	1155, 1549
HpaII	CCGG	6 cut(s)	144, 240, 279, 1097, 1218, 2144
HphI	GGTGA	7 cut(s)	247, 361, 442, 770, 885, 1205, 1988
Hpy166II	GTNNAC	6 cut(s)	986, 1155, 1321, 1549, 1837, 1847
Hpy8I	GTNNAC	6 cut(s)	986, 1155, 1321, 1549, 1837, 1847
Hpy99I	CGWCG	1 cut(s)	70
HpyAV	CCTTC	9 cut(s)	87, 130, 181, 1069, 1130, 1169, 1183, 1735, 2015
HpyCH4III	ACNGT	8 cut(s)	395, 541, 662, 931, 1325, 1531, 1564, 1969
HpyCH4IV	ACGT	2 cut(s)	68, 441
HpyF10VI	GCNNNNNNNGC	5 cut(s)	318, 932, 1829, 1874, 1927
HpyF3I	CTNAG	6 cut(s)	645, 1163, 1506, 1569, 1578, 2100
HpySE526I	ACGT	2 cut(s)	68, 441
Hsp92I	GRCGYC	2 cut(s)	68, 117
HspAI	GCGC	7 cut(s)	7, 117, 155, 157, 159, 820, 1821
KasI	GGCGCC	1 cut(s)	116
Kpn2I	TCCGGA	1 cut(s)	1096
KspAI	GTTAAC	2 cut(s)	1155, 1549
Kzo9I	GATC	9 cut(s)	53, 285, 366, 544, 613, 901, 1477, 1501, 1978
LmnI	GCTCC	4 cut(s)	175, 608, 654, 1918
Lsp1109I	GCAGC	7 cut(s)	107, 370, 652, 1769, 1852, 1910, 2185
LweI	GCATC	3 cut(s)	1242, 1337, 1513
MaeI	CTAG	2 cut(s)	860, 2171
MaeII	ACGT	2 cut(s)	68, 441
MaeIII	GTNAC	5 cut(s)	437, 805, 1040, 1078, 1564
MalI	GATC	9 cut(s)	55, 287, 368, 546, 615, 903, 1479, 1503, 1980
MauBI	CGCGCGCG	1 cut(s)	155
MbiI	CCGCTC	1 cut(s)	1512
MboI	GATC	9 cut(s)	53, 285, 366, 544, 613, 901, 1477, 1501, 1978
MboII	GAAGA	7 cut(s)	93, 295, 704, 707, 788, 1082, 2078
MfeI	CAATTG	1 cut(s)	681
MflI	RGATCY	5 cut(s)	53, 285, 613, 1477, 1501
MhlI	GDGCHC	5 cut(s)	472, 888, 1923, 2139, 2192
Mly113I	GGCGCC	1 cut(s)	117
MlyI	GAGTC	6 cut(s)	185, 230, 1658, 1832, 2024, 2092
MmeI	TCCRAC	4 cut(s)	669, 1052, 1505, 1513
Mph1103I	ATGCAT	2 cut(s)	1257, 1879
MroI	TCCGGA	1 cut(s)	1096
MroXI	GAANNNNTTC	2 cut(s)	780, 1745
MslI	CAYNNNNRTG	3 cut(s)	660, 1260, 1697
MspA1I	CMGCKG	1 cut(s)	1573
MspI	CCGG	6 cut(s)	144, 240, 279, 1097, 1218, 2144
MspR9I	CCNGG	5 cut(s)	280, 435, 619, 635, 2018
MunI	CAATTG	1 cut(s)	681
Mva1269I	GAATGC	3 cut(s)	869, 1006, 1255
MvaI	CCWGG	4 cut(s)	435, 619, 635, 2018
MvnI	CGCG	5 cut(s)	7, 155, 157, 159, 822
MwoI	GCNNNNNNNGC	5 cut(s)	318, 932, 1829, 1874, 1927
NarI	GGCGCC	1 cut(s)	117
NciI	CCSGG	1 cut(s)	280
NcoI	CCATGG	3 cut(s)	179, 195, 342
NdeII	GATC	9 cut(s)	53, 285, 366, 544, 613, 901, 1477, 1501, 1978
NmeAIII	GCCGAG	1 cut(s)	203
NmuCI	GTSAC	2 cut(s)	437, 805
NsiI	ATGCAT	2 cut(s)	1257, 1879
NspI	RCATGY	3 cut(s)	1292, 1799, 2068
PauI	GCGCGC	2 cut(s)	155, 157
PciI	ACATGT	1 cut(s)	2064
PcsI	WCGNNNNNNNCGW	1 cut(s)	297
PctI	GAATGC	3 cut(s)	869, 1006, 1255
PdmI	GAANNNNTTC	2 cut(s)	780, 1745
PfeI	GAWTC	6 cut(s)	219, 295, 338, 1167, 1430, 1459
PflFI	GACNNNGTC	1 cut(s)	1617
PflMI	CCANNNNNTGG	1 cut(s)	1834
PinAI	ACCGGT	1 cut(s)	1217
PkrI	GCNGC	7 cut(s)	97, 385, 667, 1759, 1867, 1900, 2200
PleI	GAGTC	6 cut(s)	185, 230, 1658, 1832, 2024, 2092
PluTI	GGCGCC	1 cut(s)	120
PpsI	GAGTC	6 cut(s)	185, 230, 1658, 1832, 2024, 2092
PscI	ACATGT	1 cut(s)	2064
PshAI	GACNNNNGTC	1 cut(s)	1034
PshBI	ATTAAT	2 cut(s)	1212, 1775
PsiI	TTATAA	1 cut(s)	587
Psp124BI	GAGCTC	1 cut(s)	472
Psp6I	CCWGG	4 cut(s)	433, 617, 633, 2016
PspFI	CCCAGC	1 cut(s)	1292
PspGI	CCWGG	4 cut(s)	433, 617, 633, 2016
PspPI	GGNCC	3 cut(s)	226, 749, 1103
PsuI	RGATCY	5 cut(s)	53, 285, 613, 1477, 1501
PsyI	GACNNNGTC	1 cut(s)	1617
PteI	GCGCGC	2 cut(s)	155, 157
RsaI	GTAC	6 cut(s)	397, 987, 1947, 1966, 2052, 2129
RsaNI	GTAC	6 cut(s)	396, 986, 1946, 1965, 2051, 2128
RseI	CAYNNNNRTG	3 cut(s)	660, 1260, 1697
SacI	GAGCTC	1 cut(s)	472
SatI	GCNGC	7 cut(s)	96, 384, 666, 1758, 1866, 1899, 2199
Sau3AI	GATC	9 cut(s)	53, 285, 366, 544, 613, 901, 1477, 1501, 1978
Sau96I	GGNCC	3 cut(s)	226, 749, 1103
SchI	GAGTC	6 cut(s)	185, 230, 1658, 1832, 2024, 2092
ScrFI	CCNGG	5 cut(s)	280, 435, 619, 635, 2018
SduI	GDGCHC	5 cut(s)	472, 888, 1923, 2139, 2192
SfaNI	GCATC	3 cut(s)	1242, 1337, 1513
SfcI	CTRYAG	3 cut(s)	49, 930, 1950
SfoI	GGCGCC	1 cut(s)	118
SinI	GGWCC	1 cut(s)	749
SmiMI	CAYNNNNRTG	3 cut(s)	660, 1260, 1697
SmlI	CTYRAG	1 cut(s)	905
SmoI	CTYRAG	1 cut(s)	905
SpeI	ACTAGT	1 cut(s)	2170
SsiI	CCGC	6 cut(s)	130, 652, 919, 1512, 1573, 1630
SspDI	GGCGCC	1 cut(s)	116
SspMI	CTAG	2 cut(s)	860, 2171
SstI	GAGCTC	1 cut(s)	472
StyD4I	CCNGG	5 cut(s)	278, 433, 617, 633, 2016
StyI	CCWWGG	4 cut(s)	162, 179, 195, 342
TaaI	ACNGT	8 cut(s)	395, 541, 662, 931, 1325, 1531, 1564, 1969
TaiI	ACGT	2 cut(s)	71, 444
TaqI	TCGA	5 cut(s)	58, 234, 307, 456, 2078
TaqII	GACCGA	2 cut(s)	615, 2079
TatI	WGTACW	3 cut(s)	395, 985, 2127
TfiI	GAWTC	6 cut(s)	219, 295, 338, 1167, 1430, 1459
TscAI	CASTG	6 cut(s)	667, 1401, 1534, 1849, 1914, 2200
TseFI	GTSAC	2 cut(s)	437, 805
TseI	GCWGC	7 cut(s)	95, 383, 665, 1757, 1865, 1898, 2198
Tsp45I	GTSAC	2 cut(s)	437, 805
TspDTI	ATGAA	7 cut(s)	569, 734, 743, 946, 1083, 1188, 1761
TspRI	CASTG	6 cut(s)	667, 1401, 1534, 1849, 1914, 2200
Tth111I	GACNNNGTC	1 cut(s)	1617
Van91I	CCANNNNNTGG	1 cut(s)	1834
VpaK11BI	GGWCC	1 cut(s)	749
VspI	ATTAAT	2 cut(s)	1212, 1775
XapI	RAATTY	3 cut(s)	1682, 1720, 2114
XceI	RCATGY	3 cut(s)	1292, 1799, 2068
XmnI	GAANNNNTTC	2 cut(s)	780, 1745
XspI	CTAG	2 cut(s)	860, 2171
ZraI	GACGTC	1 cut(s)	69
Zsp2I	ATGCAT	2 cut(s)	1257, 1879

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.