Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1D

Orthogroup ID

OG0012306

Physical Location & Seq

Genomic Coordinates Forward (+)

40517091 .. 40524825

Gene Structure 7735 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1DG207300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2223 bp

ATGCCCCGCTTCCCTCGCTTCTCCACCCCACGCCTCCTCTACTCCCTCCACACCTCCAAAACGACGCCGTCCCGCCCATCCCGGTCCTCCTCCTCCTCTCTCCTCGTCGGCTCCTTCCACCTCCGCCAATTCTCCGCCGGCAACCTCGCACGTGCCAAGGACGACAAGGAGCTATGGTGGACGGACGTGATGGCCAGGCTCCGCAACATCGGAATCTCGGCCCATATCGACTCCGGCAAGACCACTCTCACCGAGAGAATTCTCTACTACACCGGAAAGATCCACGAGATTCACGAGGTCAGAGGCCGAGACGGCGTCGGCGCCAAAATGGACTCCATGGATTTGGAGAGAGAGAAGGGAATTACCATTCAGTCCGCTGCCACTTACTGCACTTGGAATGGTTATCAGATTAACATAATTGACACCCCTGGTCACGTTGATTTCACCATTGAGGTTGAGAGAGCTTTGCGTGTTCTTGATGGAGCCATCCTTGTCCTTTGTAGCGTTGGTGGTGTACAGAGTCAGTCAATTACTGTTGATCGGCAAATGAAAAGATATGAGGTTCCAAGAATTGCATTTATAAACAAACTTGATCGAATGGGAGCAGATCCATGGAAAGTTCTGAATCAGATGAGGTCTAAGCTCCGGCATCATGCTGCTGCATTGCAACTTCCTATTGGGTTGGAAGATGATTTTAAGGGTCTTATTGATCTTGTGCAGTTAAAAGCTTTGTATTTTCATGGTTCTAGCGGTCAGGAAATTGTCATTGAAGACATTCCTTCTGATATGGAGGCGTTAGTCGCAGAAAAAAGGCGTGAGCTAATTGAAATTGTTTCCGAAGTTGATGACCAACTTGCTGAAGCATTTCTTGAAGATGTGCCCATTACACCTACTGATCTGGAGGAAGCTGTGCGAAGGGCTACCATCGCAAGGAAATTTATACCTGTGTTCATGGGTAGTGCATTTAAAAACAAGGGTGTACAACCACTTTTGAATGGTGTACTTGGTTATTTGCCTTGTCCATCTGAAGTCAGTAACTATGCTCTTGACCAGACTAAGGATGAAGAGAAGGTTATATTAGGTGGAACTCCAGATGGACCTCTTGTAGCATTGGCCTTTAAATTGGAGGAAGGGCGTTTTGGTCAGTTAACCTTTCTAAGAATCTATGAAGGTGTTATACGGAAGGGTGATTTCATTTTTAACATTAACACTGGCAAGAAGATTAAGGTTCCTCGCTTGGTCCGGATGCATTCTGATGAGATGGAGGATATTCAAGAGGCACATGCTGGACAAATAGTCGCTGTATTTGGTGTGGATTGTGCATCAGGAGATACATTTACTGACGGTTCAGTTAAATATACCATGACTTCTATGAATGTCCCTGAGCCAGTGATGTCATTAGCTGTTCAGCCAGTTTCAAAAGATTCTGGAGGACAATTTTCGAAGGCTTTGAATCGTTTTCAGAAAGAAGATCCTACCTTCCGTGTTGGCTTGGACCCTGAGAGCGGACAGACAATCATATCAGGAATGGGAGAGCTGCATTTGGAAGTTTATGTTGAACGCATTCGGAGAGAATATAAGGTTGATGCAACTGTTGGAAAGCCTCGTGTAAACTTCAGAGAGACTGTTACTCAACGTGCTGATTTTGATTATTTACATAAAAAGCAATCTGGAGGACAGGGTCAATATGGGCGAGTAACTGGATTTATCGAACCGCTGCCTGCAGGATCAGCGACAAAGTTTGAATTTGAAAACAGTATTGTGGGACAAGCTATACCATCAAATTTTATCCCAGCTATTGAGAAGGGTTTTAAAGAAGCAGCCAATTCGGGCTCATTAATTGGCCATCCAGTTGAAAATGTTCGCATTGTTTTAACTGATGGTGCTTCCCATGCTGTGGATTCTAGTGAACTCGCCTTCAAATTAGCTGCAATATATGCCTTTAGAAAGTGCTATACGGTGGCAAAGCCGGTGATACTGGAACCTGTTATGTTGGTGGAGTTGAAAGTACCTACAGAATTTCAGGGCACTGTTGCTGGTGATATTAACAAGAGAAAAGGTGTCATTATTGGAAATGATCAGGAAGGGGATGACTCTGTTATTACGGCCCAAGTAAGTATTTTCTTTTATGTAATTCTACTTCATACATTTACTTTGCTTATGCAACAAAAGGGGTTTTCACAGGTAATTAAGGACACTATTGTACTTGAAATCTTAAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003676 GO:0003723 GO:0003746 GO:0003824 GO:0003924 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006412 GO:0006414 GO:0006518 GO:0006807 GO:0008135 GO:0008144 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019538 GO:0030554 GO:0032543 GO:0032553 GO:0032555 GO:0032559 GO:0034641 GO:0034645 GO:0035639 GO:0036094 GO:0042221 GO:0043043 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0050896 GO:0070125 GO:0071704 GO:0097159 GO:0097367 GO:0140053 GO:1901265 GO:1901363 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EFG_C EFG_II EFG_IV GTP_EFTU GTP_EFTU_D2

Protein Analysis

740

Amino Acids

82.1

Weight (kDa)

6.2

Isoelectric Point (pI)

37.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GTP_EFTU	PF00009	66 - 339	8.6e-61	Elongation factor Tu GTP binding domain
EF-G_D2	PF22042	380 - 448	8.2e-11	Elongation factor G domain 2
GTP_EFTU_D2	PF03144	381 - 447	4.4e-14	Elongation factor Tu domain 2
EFG_III	PF14492	460 - 534	2.5e-29	Elongation Factor G, domain III
EFG_IV	PF03764	535 - 655	6.1e-38	Elongation factor G, domain IV
EFG_C	PF00679	659 - 705	7.6e-09	Elongation factor G C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0012306)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G45332 AT1G45332 AT2G45030
fragaria_vesca	FvH4_7g10500 FvH4_7g10500 FvH4_7g10500
malus_domestica	MD07G1111000.v1.1
prunus_persica	Prupe.2G134500_v2.0.a1
pyrus_communis	pycom07g09580
rosa_chinensis	RchiOBHm_Chr1g0348291
rosa_laevigata	RLG00000028669
rosa_multiflora	Rmu_sc0027364.1_g000002
rosa_roxburghii	Rroxscaffold_4G00307040
rosa_rugosa	Rorug01G0194100
rosa_samantha	Rh1AG211300 Rh1BG177800 Rh1CG195700 Rh1DG207300
rosa_wichuraiana	Rw1G017850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	581
AccB1I	GGYRCC	1 cut(s)	320
AccB7I	CCANNNNNTGG	1 cut(s)	1897
AccBSI	CCGCTC	1 cut(s)	1506
AccIII	TCCGGA	1 cut(s)	1242
AciI	CCGC	9 cut(s)	7, 73, 124, 135, 202, 375, 750, 1506, 1715
AclWI	GGATC	4 cut(s)	274, 602, 1466, 1735
AcoI	YGGCCR	2 cut(s)	192, 1843
AcsI	RAATTY	5 cut(s)	258, 935, 1745, 1783, 2018
AcuI	CTGAAG	3 cut(s)	879, 1047, 1600
AcvI	CACGTG	1 cut(s)	152
AcyI	GRCGYC	3 cut(s)	65, 315, 321
AfaI	GTAC	5 cut(s)	516, 981, 1002, 2010, 2205
AfiI	CCNNNNNNNGG	4 cut(s)	930, 1057, 1505, 1897
AhdI	GACNNNNNGTC	1 cut(s)	1295
AjiI	CACGTC	1 cut(s)	187
AjnI	CCWGG	2 cut(s)	194, 427
AjuI	GAANNNNNNNTTGG	2 cut(s)	1122, 1154
Alw26I	GTCTC	2 cut(s)	303, 1616
AlwI	GGATC	4 cut(s)	274, 602, 1466, 1735
Aor13HI	TCCGGA	1 cut(s)	1242
AoxI	GGCC	6 cut(s)	192, 219, 304, 1113, 1843, 2106
ApeKI	GCWGC	7 cut(s)	377, 656, 659, 1537, 1717, 1820, 1928
ApoI	RAATTY	5 cut(s)	258, 935, 1745, 1783, 2018
AseI	ATTAAT	1 cut(s)	1838
Asp700I	GAANNNNTTC	4 cut(s)	774, 864, 1563, 1860
AspLEI	GCGC	1 cut(s)	323
AspS9I	GGNCC	6 cut(s)	84, 220, 1097, 1240, 1495, 2107
AsuC2I	CCSGG	1 cut(s)	82
AsuHPI	GGTGA	5 cut(s)	241, 436, 1199, 1984, 2051
AsuII	TTCGAA	1 cut(s)	1442
AvaII	GGWCC	4 cut(s)	84, 1097, 1240, 1495
BaeGI	GKGCMC	2 cut(s)	882, 2030
BalI	TGGCCA	2 cut(s)	194, 1845
BanI	GGYRCC	1 cut(s)	320
BanII	GRGCYC	1 cut(s)	1835
BarI	GAAGNNNNNNTAC	4 cut(s)	1161, 1193, 2124, 2156
BauI	CACGAG	3 cut(s)	284, 293, 1605
BbrPI	CACGTG	1 cut(s)	152
BbsI	GAAGAC	1 cut(s)	777
BbvI	GCAGC	7 cut(s)	364, 643, 646, 1524, 1704, 1832, 1915
BceAI	ACGGC	3 cut(s)	52, 328, 2121
BcgI	CGANNNNNNTGC	2 cut(s)	1809, 1843
BciT130I	CCWGG	2 cut(s)	196, 429
BclI	TGATCA	1 cut(s)	2077
BcnI	CCSGG	1 cut(s)	82
BcoDI	GTCTC	2 cut(s)	303, 1616
BfaI	CTAG	2 cut(s)	747, 1905
BfmI	CTRYAG	2 cut(s)	1722, 2013
BfoI	RGCGCY	1 cut(s)	324
BglI	GCCNNNNNGGC	1 cut(s)	312
BisI	GCNGC	7 cut(s)	378, 657, 660, 1538, 1718, 1821, 1929
BlsI	GCNGC	7 cut(s)	379, 658, 661, 1539, 1719, 1822, 1930
Bme1390I	CCNGG	3 cut(s)	82, 196, 429
Bme18I	GGWCC	4 cut(s)	84, 1097, 1240, 1495
BmeRI	GACNNNNNGTC	1 cut(s)	1295
BmgBI	CACGTC	1 cut(s)	187
BmgT120I	GGNCC	6 cut(s)	84, 220, 1097, 1240, 1495, 2107
BmiI	GGNNCC	8 cut(s)	112, 200, 322, 484, 564, 1230, 1497, 1983
BmrFI	CCNGG	3 cut(s)	82, 196, 429
BmsI	GCATC	4 cut(s)	658, 1236, 1331, 1576
BpiI	GAAGAC	1 cut(s)	777
BpmI	CTGGAG	4 cut(s)	920, 1074, 1449, 1692
Bpu10I	CCTNAGC	1 cut(s)	1383
Bpu14I	TTCGAA	1 cut(s)	1442
BpuMI	CCSGG	1 cut(s)	82
BsaAI	YACGTR	1 cut(s)	152
BsaHI	GRCGYC	3 cut(s)	65, 315, 321
BsaJI	CCNNGG	4 cut(s)	156, 336, 427, 611
BsaWI	WCCGGW	2 cut(s)	272, 1242
BsaXI	ACNNNNNCTCC	2 cut(s)	161, 191
Bsc4I	CCNNNNNNNGG	4 cut(s)	930, 1057, 1505, 1897
Bse118I	RCCGGY	2 cut(s)	137, 1969
Bse1I	ACTGG	6 cut(s)	1216, 1388, 1412, 1705, 1850, 1983
Bse3DI	GCAATG	1 cut(s)	662
BseAI	TCCGGA	1 cut(s)	1242
BseBI	CCWGG	2 cut(s)	196, 429
BseDI	CCNNGG	4 cut(s)	156, 336, 427, 611
BseGI	GGATG	6 cut(s)	77, 486, 1066, 1251, 1846, 2095
BseLI	CCNNNNNNNGG	4 cut(s)	930, 1057, 1505, 1897
BseMI	GCAATG	1 cut(s)	662
BseMII	CTCAG	2 cut(s)	1374, 1491
BseNI	ACTGG	6 cut(s)	1216, 1388, 1412, 1705, 1850, 1983
BseRI	GAGGAG	5 cut(s)	26, 79, 82, 85, 92
BseSI	GKGCMC	2 cut(s)	882, 2030
BseXI	GCAGC	7 cut(s)	364, 643, 646, 1524, 1704, 1832, 1915
BseYI	CCCAGC	1 cut(s)	1792
BsgI	GTGCAG	2 cut(s)	373, 737
BshFI	GGCC	6 cut(s)	194, 221, 306, 1115, 1845, 2108
BshNI	GGYRCC	1 cut(s)	320
BsiSI	CCGG	7 cut(s)	82, 138, 234, 273, 646, 1243, 1970
BslFI	GGGAC	3 cut(s)	55, 1364, 1779
BslI	CCNNNNNNNGG	4 cut(s)	930, 1057, 1505, 1897
BsmAI	GTCTC	2 cut(s)	303, 1616
BsmBI	CGTCTC	1 cut(s)	303
BsmFI	GGGAC	3 cut(s)	55, 1364, 1779
BsmI	GAATGC	2 cut(s)	1249, 1563
BsnI	GGCC	6 cut(s)	194, 221, 306, 1115, 1845, 2108
Bsp119I	TTCGAA	1 cut(s)	1442
Bsp1286I	GDGCHC	3 cut(s)	882, 1835, 2030
Bsp13I	TCCGGA	1 cut(s)	1242
Bsp1407I	TGTACA	2 cut(s)	514, 979
Bsp143I	GATC	9 cut(s)	279, 538, 592, 607, 709, 895, 1471, 1727, 2077
Bsp19I	CCATGG	2 cut(s)	336, 611
BspACI	CCGC	9 cut(s)	7, 73, 124, 135, 202, 375, 750, 1506, 1715
BspANI	GGCC	6 cut(s)	194, 221, 306, 1115, 1845, 2108
BspCNI	CTCAG	2 cut(s)	1375, 1492
BspEI	TCCGGA	1 cut(s)	1242
BspLI	GGNNCC	8 cut(s)	112, 200, 322, 484, 564, 1230, 1497, 1983
BspMAI	CTGCAG	1 cut(s)	1726
BspPI	GGATC	4 cut(s)	274, 602, 1466, 1735
BspT104I	TTCGAA	1 cut(s)	1442
BspT107I	GGYRCC	1 cut(s)	320
BsrBI	CCGCTC	1 cut(s)	1506
BsrDI	GCAATG	1 cut(s)	662
BsrFI	RCCGGY	2 cut(s)	137, 1969
BsrGI	TGTACA	2 cut(s)	514, 979
BsrI	ACTGG	6 cut(s)	1216, 1388, 1412, 1705, 1850, 1983
BssAI	RCCGGY	2 cut(s)	137, 1969
BssECI	CCNNGG	4 cut(s)	156, 336, 427, 611
BssMI	GATC	9 cut(s)	279, 538, 592, 607, 709, 895, 1471, 1727, 2077
BssNI	GRCGYC	3 cut(s)	65, 315, 321
BssSI	CACGAG	3 cut(s)	284, 293, 1605
BssT1I	CCWWGG	3 cut(s)	156, 336, 611
Bst2BI	CACGAG	3 cut(s)	284, 293, 1605
Bst2UI	CCWGG	2 cut(s)	196, 429
Bst4CI	ACNGT	7 cut(s)	535, 1346, 1594, 1627, 1757, 1960, 2032
Bst6I	CTCTTC	1 cut(s)	1059
BstACI	GRCGYC	3 cut(s)	65, 315, 321
BstAPI	GCANNNNNTGC	1 cut(s)	1937
BstAUI	TGTACA	2 cut(s)	514, 979
BstBAI	YACGTR	1 cut(s)	152
BstBI	TTCGAA	1 cut(s)	1442
BstC8I	GCNNGC	2 cut(s)	139, 1722
BstDEI	CTNAG	5 cut(s)	639, 1056, 1157, 1383, 1500
BstDSI	CCRYGG	2 cut(s)	336, 611
BstF5I	GGATG	6 cut(s)	77, 486, 1066, 1251, 1846, 2095
BstH2I	RGCGCY	1 cut(s)	324
BstHHI	GCGC	1 cut(s)	323
BstKTI	GATC	9 cut(s)	282, 541, 595, 610, 712, 898, 1474, 1730, 2080
BstMAI	GTCTC	2 cut(s)	303, 1616
BstMBI	GATC	9 cut(s)	279, 538, 592, 607, 709, 895, 1471, 1727, 2077
BstMWI	GCNNNNNNNGC	7 cut(s)	15, 312, 800, 926, 1730, 1892, 1937
BstNI	CCWGG	2 cut(s)	196, 429
BstNSI	RCATGY	1 cut(s)	1286
BstSCI	CCNGG	3 cut(s)	80, 194, 427
BstSFI	CTRYAG	2 cut(s)	1722, 2013
BstSLI	GKGCMC	2 cut(s)	882, 2030
BstV1I	GCAGC	7 cut(s)	364, 643, 646, 1524, 1704, 1832, 1915
BstV2I	GAAGAC	1 cut(s)	777
BstX2I	RGATCY	3 cut(s)	279, 607, 1471
BstXI	CCANNNNNNTGG	1 cut(s)	343
BstYI	RGATCY	3 cut(s)	279, 607, 1471
BsuRI	GGCC	6 cut(s)	194, 221, 306, 1115, 1845, 2108
BtgI	CCRYGG	2 cut(s)	336, 611
BtgZI	GCGATG	1 cut(s)	910
BtrI	CACGTC	1 cut(s)	187
BtsCI	GGATG	6 cut(s)	77, 486, 1066, 1251, 1846, 2095
BtsIMutI	CAGTG	3 cut(s)	1209, 1395, 2028
Cac8I	GCNNGC	2 cut(s)	139, 1722
CfoI	GCGC	1 cut(s)	323
Cfr10I	RCCGGY	2 cut(s)	137, 1969
Cfr13I	GGNCC	6 cut(s)	84, 220, 1097, 1240, 1495, 2107
CseI	GACGC	2 cut(s)	73, 304
Csp6I	GTAC	5 cut(s)	515, 980, 1001, 2009, 2204
CviAII	CATG	8 cut(s)	337, 612, 653, 740, 952, 1283, 1363, 1892
CviQI	GTAC	5 cut(s)	515, 980, 1001, 2009, 2204
DdeI	CTNAG	5 cut(s)	639, 1056, 1157, 1383, 1500
DinI	GGCGCC	1 cut(s)	322
DpnI	GATC	9 cut(s)	281, 540, 594, 609, 711, 897, 1473, 1729, 2079
DpnII	GATC	9 cut(s)	279, 538, 592, 607, 709, 895, 1471, 1727, 2077
DraI	TTTAAA	3 cut(s)	967, 1120, 1813
DriI	GACNNNNNGTC	1 cut(s)	1295
EaeI	YGGCCR	2 cut(s)	192, 1843
Eam1104I	CTCTTC	1 cut(s)	1059
Eam1105I	GACNNNNNGTC	1 cut(s)	1295
EarI	CTCTTC	1 cut(s)	1059
EciI	GGCGGA	2 cut(s)	113, 124
Eco130I	CCWWGG	3 cut(s)	156, 336, 611
Eco24I	GRGCYC	1 cut(s)	1835
Eco47I	GGWCC	4 cut(s)	84, 1097, 1240, 1495
Eco57I	CTGAAG	3 cut(s)	879, 1047, 1600
Eco72I	CACGTG	1 cut(s)	152
EcoRI	GAATTC	1 cut(s)	258
EcoRII	CCWGG	2 cut(s)	194, 427
EcoT14I	CCWWGG	3 cut(s)	156, 336, 611
EcoT22I	ATGCAT	1 cut(s)	1251
EcoT38I	GRGCYC	1 cut(s)	1835
EgeI	GGCGCC	1 cut(s)	322
EheI	GGCGCC	1 cut(s)	322
ErhI	CCWWGG	3 cut(s)	156, 336, 611
Esp3I	CGTCTC	1 cut(s)	303
FaeI	CATG	8 cut(s)	340, 615, 656, 743, 955, 1286, 1366, 1895
FalI	AAGNNNNNCTT	2 cut(s)	852, 884
FaqI	GGGAC	3 cut(s)	55, 1364, 1779
FatI	CATG	8 cut(s)	336, 611, 652, 739, 951, 1282, 1362, 1891
FauI	CCCGC	2 cut(s)	14, 80
FbaI	TGATCA	1 cut(s)	2077
Fnu4HI	GCNGC	7 cut(s)	378, 657, 660, 1538, 1718, 1821, 1929
FokI	GGATG	6 cut(s)	64, 473, 1073, 1258, 1833, 2102
FriOI	GRGCYC	1 cut(s)	1835
Fsp4HI	GCNGC	7 cut(s)	378, 657, 660, 1538, 1718, 1821, 1929
FspBI	CTAG	2 cut(s)	747, 1905
GlaI	GCGC	1 cut(s)	322
GluI	GCNGC	7 cut(s)	378, 657, 660, 1538, 1718, 1821, 1929
GsaI	CCCAGC	1 cut(s)	1796
GsuI	CTGGAG	4 cut(s)	920, 1074, 1449, 1692
HaeII	RGCGCY	1 cut(s)	324
HaeIII	GGCC	6 cut(s)	194, 221, 306, 1115, 1845, 2108
HapII	CCGG	7 cut(s)	82, 138, 234, 273, 646, 1243, 1970
HgaI	GACGC	2 cut(s)	73, 304
HhaI	GCGC	1 cut(s)	323
Hin1I	GRCGYC	3 cut(s)	65, 315, 321
Hin1II	CATG	8 cut(s)	340, 615, 656, 743, 955, 1286, 1366, 1895
Hin6I	GCGC	1 cut(s)	321
HinP1I	GCGC	1 cut(s)	321
HincII	GTYRAC	1 cut(s)	1149
HindII	GTYRAC	1 cut(s)	1149
HindIII	AAGCTT	1 cut(s)	726
HpaI	GTTAAC	1 cut(s)	1149
HpaII	CCGG	7 cut(s)	82, 138, 234, 273, 646, 1243, 1970
HphI	GGTGA	5 cut(s)	241, 436, 1199, 1984, 2051
Hpy166II	GTNNAC	7 cut(s)	180, 515, 980, 1001, 1149, 1612, 1910
Hpy8I	GTNNAC	7 cut(s)	180, 515, 980, 1001, 1149, 1612, 1910
Hpy99I	CGWCG	3 cut(s)	67, 110, 320
HpyCH4III	ACNGT	7 cut(s)	535, 1346, 1594, 1627, 1757, 1960, 2032
HpyCH4IV	ACGT	4 cut(s)	151, 186, 435, 1636
HpyF10VI	GCNNNNNNNGC	7 cut(s)	15, 312, 800, 926, 1730, 1892, 1937
HpyF3I	CTNAG	5 cut(s)	639, 1056, 1157, 1383, 1500
HpySE526I	ACGT	4 cut(s)	151, 186, 435, 1636
Hsp92I	GRCGYC	3 cut(s)	65, 315, 321
Hsp92II	CATG	8 cut(s)	340, 615, 656, 743, 955, 1286, 1366, 1895
HspAI	GCGC	1 cut(s)	321
KasI	GGCGCC	1 cut(s)	320
Kpn2I	TCCGGA	1 cut(s)	1242
KroI	GCCGGC	1 cut(s)	137
KroNI	GCCGGC	1 cut(s)	139
Ksp22I	TGATCA	1 cut(s)	2077
KspAI	GTTAAC	1 cut(s)	1149
Kzo9I	GATC	9 cut(s)	279, 538, 592, 607, 709, 895, 1471, 1727, 2077
LmnI	GCTCC	6 cut(s)	116, 169, 204, 482, 602, 648
Lsp1109I	GCAGC	7 cut(s)	364, 643, 646, 1524, 1704, 1832, 1915
LweI	GCATC	4 cut(s)	658, 1236, 1331, 1576
MaeI	CTAG	2 cut(s)	747, 1905
MaeII	ACGT	4 cut(s)	151, 186, 435, 1636
MaeIII	GTNAC	4 cut(s)	431, 1034, 1627, 1696
MalI	GATC	9 cut(s)	281, 540, 594, 609, 711, 897, 1473, 1729, 2079
MbiI	CCGCTC	1 cut(s)	1506
MboI	GATC	9 cut(s)	279, 538, 592, 607, 709, 895, 1471, 1727, 2077
MboII	GAAGA	6 cut(s)	698, 782, 884, 1076, 1231, 1481
MflI	RGATCY	3 cut(s)	279, 607, 1471
MhlI	GDGCHC	3 cut(s)	882, 1835, 2030
MlsI	TGGCCA	2 cut(s)	194, 1845
MluNI	TGGCCA	2 cut(s)	194, 1845
Mly113I	GGCGCC	1 cut(s)	321
MlyI	GAGTC	4 cut(s)	224, 326, 529, 2087
MmeI	TCCRAC	2 cut(s)	663, 1576
Mox20I	TGGCCA	2 cut(s)	194, 1845
Mph1103I	ATGCAT	1 cut(s)	1251
MroI	TCCGGA	1 cut(s)	1242
MroNI	GCCGGC	1 cut(s)	137
MroXI	GAANNNNTTC	4 cut(s)	774, 864, 1563, 1860
MscI	TGGCCA	2 cut(s)	194, 1845
MslI	CAYNNNNRTG	1 cut(s)	1254
Msp20I	TGGCCA	2 cut(s)	194, 1845
MspA1I	CMGCKG	2 cut(s)	377, 1717
MspI	CCGG	7 cut(s)	82, 138, 234, 273, 646, 1243, 1970
MspR9I	CCNGG	3 cut(s)	82, 196, 429
Mva1269I	GAATGC	2 cut(s)	1249, 1563
MvaI	CCWGG	2 cut(s)	196, 429
MwoI	GCNNNNNNNGC	7 cut(s)	15, 312, 800, 926, 1730, 1892, 1937
NaeI	GCCGGC	1 cut(s)	139
NarI	GGCGCC	1 cut(s)	321
NciI	CCSGG	1 cut(s)	82
NcoI	CCATGG	2 cut(s)	336, 611
NdeII	GATC	9 cut(s)	279, 538, 592, 607, 709, 895, 1471, 1727, 2077
NgoMIV	GCCGGC	1 cut(s)	137
NlaIII	CATG	8 cut(s)	340, 615, 656, 743, 955, 1286, 1366, 1895
NlaIV	GGNNCC	8 cut(s)	112, 200, 322, 484, 564, 1230, 1497, 1983
NmeAIII	GCCGAG	2 cut(s)	197, 332
NmuCI	GTSAC	1 cut(s)	431
NsiI	ATGCAT	1 cut(s)	1251
NspI	RCATGY	1 cut(s)	1286
NspV	TTCGAA	1 cut(s)	1442
PcsI	WCGNNNNNNNCGW	1 cut(s)	291
PctI	GAATGC	2 cut(s)	1249, 1563
PdiI	GCCGGC	1 cut(s)	139
PdmI	GAANNNNTTC	4 cut(s)	774, 864, 1563, 1860
PfeI	GAWTC	7 cut(s)	213, 289, 625, 1161, 1424, 1453, 1901
PflFI	GACNNNGTC	3 cut(s)	67, 314, 1680
PflMI	CCANNNNNTGG	1 cut(s)	1897
PkrI	GCNGC	7 cut(s)	379, 658, 661, 1539, 1719, 1822, 1930
PleI	GAGTC	4 cut(s)	224, 326, 528, 2087
PluTI	GGCGCC	1 cut(s)	324
PmaCI	CACGTG	1 cut(s)	152
PmlI	CACGTG	1 cut(s)	152
PpsI	GAGTC	4 cut(s)	224, 326, 528, 2087
Ppu21I	YACGTR	1 cut(s)	152
PshBI	ATTAAT	1 cut(s)	1838
PsiI	TTATAA	1 cut(s)	581
Psp6I	CCWGG	2 cut(s)	194, 427
PspCI	CACGTG	1 cut(s)	152
PspFI	CCCAGC	1 cut(s)	1792
PspGI	CCWGG	2 cut(s)	194, 427
PspN4I	GGNNCC	8 cut(s)	112, 200, 322, 484, 564, 1230, 1497, 1983
PspPI	GGNCC	6 cut(s)	84, 220, 1097, 1240, 1495, 2107
PstI	CTGCAG	1 cut(s)	1726
PsuI	RGATCY	3 cut(s)	279, 607, 1471
PsyI	GACNNNGTC	3 cut(s)	67, 314, 1680
RsaI	GTAC	5 cut(s)	516, 981, 1002, 2010, 2205
RsaNI	GTAC	5 cut(s)	515, 980, 1001, 2009, 2204
RseI	CAYNNNNRTG	1 cut(s)	1254
SatI	GCNGC	7 cut(s)	378, 657, 660, 1538, 1718, 1821, 1929
Sau3AI	GATC	9 cut(s)	279, 538, 592, 607, 709, 895, 1471, 1727, 2077
Sau96I	GGNCC	6 cut(s)	84, 220, 1097, 1240, 1495, 2107
SbfI	CCTGCAGG	1 cut(s)	1726
SchI	GAGTC	4 cut(s)	224, 326, 529, 2087
ScrFI	CCNGG	3 cut(s)	82, 196, 429
SdaI	CCTGCAGG	1 cut(s)	1726
SduI	GDGCHC	3 cut(s)	882, 1835, 2030
SfaNI	GCATC	4 cut(s)	658, 1236, 1331, 1576
SfcI	CTRYAG	2 cut(s)	1722, 2013
SfoI	GGCGCC	1 cut(s)	322
SfuI	TTCGAA	1 cut(s)	1442
SinI	GGWCC	4 cut(s)	84, 1097, 1240, 1495
SmiMI	CAYNNNNRTG	1 cut(s)	1254
Sse8387I	CCTGCAGG	1 cut(s)	1726
SsiI	CCGC	9 cut(s)	7, 73, 124, 135, 202, 375, 750, 1506, 1715
SspDI	GGCGCC	1 cut(s)	320
SspMI	CTAG	2 cut(s)	747, 1905
StyD4I	CCNGG	3 cut(s)	80, 194, 427
StyI	CCWWGG	3 cut(s)	156, 336, 611
TaaI	ACNGT	7 cut(s)	535, 1346, 1594, 1627, 1757, 1960, 2032
TaiI	ACGT	4 cut(s)	154, 189, 438, 1639
TaqI	TCGA	4 cut(s)	228, 595, 1442, 1710
TatI	WGTACW	4 cut(s)	514, 979, 1000, 2203
TfiI	GAWTC	7 cut(s)	213, 289, 625, 1161, 1424, 1453, 1901
TscAI	CASTG	3 cut(s)	1216, 1395, 2035
TseFI	GTSAC	1 cut(s)	431
TseI	GCWGC	7 cut(s)	377, 656, 659, 1537, 1717, 1820, 1928
Tsp45I	GTSAC	1 cut(s)	431
TspDTI	ATGAA	8 cut(s)	563, 728, 940, 1077, 1182, 1183, 1388, 2132
TspGWI	ACGGA	3 cut(s)	197, 1195, 1472
TspRI	CASTG	3 cut(s)	1216, 1395, 2035
Tth111I	GACNNNGTC	3 cut(s)	67, 314, 1680
Van91I	CCANNNNNTGG	1 cut(s)	1897
VpaK11BI	GGWCC	4 cut(s)	84, 1097, 1240, 1495
VspI	ATTAAT	1 cut(s)	1838
XapI	RAATTY	5 cut(s)	258, 935, 1745, 1783, 2018
XceI	RCATGY	1 cut(s)	1286
XmnI	GAANNNNTTC	4 cut(s)	774, 864, 1563, 1860
XspI	CTAG	2 cut(s)	747, 1905
Zsp2I	ATGCAT	1 cut(s)	1251

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.