Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0012306

Physical Location & Seq

Genomic Coordinates Reverse (-)

25925367 .. 25932648

Gene Structure 7282 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000028669

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2256 bp

ATGCCCCGCTTCCCCCGCTTCTCCACCCCACGCCTCCTCTACTCCCTCCACACCTCCAAAACGACGCCGTCCCCGTCCTCCTCTCTCCTCGTCGGCTCCTTCCACCTCCGCCAATTCTCCGCCGGCAACCTCGCACGTGCCAAGGACGACAAGGAGCTATGGTGGACGGACGTGATGGCCAGGCTCCGCAACATCGGAATCTCGGCCCATATCGACTCCGGCAAGACCACCCTCACCGAGAGAATTCTCTACTACACCGGAAAGATCCACGAGATTCATGAGGTCAGAGGCCGAGACGGCGTCGGCGCCAAAATGGACTCCATGGATTTGGAGAGAGAGAAGGGAATTACCATTCAGTCCGCTGCCACTTACTGCACTTGGAACGGTTATCAGATTAACATAATTGACACCCCTGGTCACGTTGATTTCACCATTGAGGTTGAGAGAGCTTTGCGTGTTCTTGATGGAGCCATCCTTGTCCTTTGTAGTGTTGGTGGTGTACAGAGTCAGTCAATTACTGTTGATCGGCAAATGAAAAGATACGAGGTTCCAAGAATTGCATTTATAAACAAACTTGATCGAATGGGAGCAGATCCATGGAAAGTTCTGAATCAGATGAGGTCTAAGCTCCGGCATCATGCTGCTGCATTGCAACTGCCTATTGGGTTGGAAGATGATTTTAAGGGTCTTATTGATCTTGTGCAGTTAAAAGCTTTGTATTTTCATGGTTCTAGCGGTCAGGAAATTGTCATTGAAGACATTCCTTCTGATATGGAGGCGTTAGTCGCGGAAAAAAGGCGTGAGCTAATTGAAATTGTTTCCGAAGTTGATGACCAACTTGCTGAAGCATTTCTTGAAGATGTGCCCATTACACCTACTGATCTGGAGGAAGCTGTGCGAAGGGCTACCATCGCAAGGAAATTTATACCTGTGTTCATGGGTAGTGCATTTAAAAACAAGGGTGTACAACCACTTTTGAATGGTGTACTTGGTTATTTGCCTTGTCCATCTGAAGTCAGTAACTATGCTCTTGACCAGACTAAGGATGAAGAGAAGGTTATATTAGGTGGAACTCCAGATGGACCTCTTGTAGCATTGGCCTTTAAATTGGAGGAAGGGCGTTTTGGTCAGTTAACCTTTCTAAGAATCTATGAAGGTGTTATACGGAAGGGTGATTTCATTTTTAACATTAACACTGGCAAGAAGATTAAGGTTCCTCGCTTGGTTCGGATGCATTCTGATGAGATGGAGGATATTCAAGAGGCACATGCTGGACAAATAGTTGCTGTATTTGGTGTGGATTGTGCATCAGGAGATACATTTACTGACGGTTCAGTTAAATATACCATGACTTCTATGAATGTCCCTGAGCCAGTGATGTCATTAGCTGTTCAGCCAGTTTCAAAAGATTCAGGAGGACAATTTTCGAAGGCTTTGAATCGTTTTCAGAAAGAAGATCCTACCTTCCGTGTTGGCTTGGACCCTGAGAGCGGACAGACAATCATATCAGGAATGGGAGAGCTGCATTTGGAAGTTTATGTTGAACGCATTCGGAGAGAATATAAGGTTGATGCAACTGTTGGAAAGCCTCGTGTAAACTTCAGAGAGACTGTTACTCAACGTGCTGACTTTGATTATTTACATAAAAAGCAATCTGGAGGACAGGGTCAATATGGGCGAGTAACTGGATTTATAGAACCGCTGCCTGCAGGATCAGCGACAAAGTTTGAATTTGAAAACAGTATTGTGGGACAAGCTATACCATCAAATTTTATCCCAGCTATTGAGAAGGGTTTTAAAGAAGCAGCCAATTCGGGCTCATTAATTGGCCATCCAGTTGAAAATGTTCGCATTGTTTTAACTGATGGTGCTTCCCATGCTGTGGATTCTAGTGAACTTGCCTTCAAATTAGCTGCAATATATGCCTTTAGAAAGTGCTATACGGTGGCGAAGCCGGTGATACTGGAGCCTGTTATGTTGGTGGAGTTGAAAGTACCTATAGAATTTCAGGGCACTGTTGCTGGTGATATTAACAAGAGAAAAGGTGTCATTATTGGAAATGATCAGGAAGGGGATGACTCTGTTATTACGGCCCAAGTCCCTCTGAACAACATGTTTGGCTATTCGACGTCTCTTCGATCAATGACACAGGGAAAAGGCGAATTCACAATGGAGTACAAAGAGCACTCGCCCGTTTCTCATGATGTGCAGGCGCAATTAGTAAACAATTACAAGGGACCCAATGCCACTGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003676 GO:0003723 GO:0003746 GO:0003824 GO:0003924 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006412 GO:0006414 GO:0006518 GO:0006807 GO:0008135 GO:0008144 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0010035 GO:0010038 GO:0010467 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019538 GO:0030554 GO:0032543 GO:0032553 GO:0032555 GO:0032559 GO:0034641 GO:0034645 GO:0035639 GO:0036094 GO:0042221 GO:0043043 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0046686 GO:0050896 GO:0070125 GO:0071704 GO:0097159 GO:0097367 GO:0140053 GO:1901265 GO:1901363 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EFG_C EFG_II EFG_IV GTP_EFTU GTP_EFTU_D2

Protein Analysis

752

Amino Acids

83.15

Weight (kDa)

5.95

Isoelectric Point (pI)

36.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GTP_EFTU	PF00009	61 - 334	8.8e-61	Elongation factor Tu GTP binding domain
EF-G_D2	PF22042	375 - 443	8.4e-11	Elongation factor G domain 2
GTP_EFTU_D2	PF03144	376 - 442	4.5e-14	Elongation factor Tu domain 2
EFG_III	PF14492	455 - 529	2.6e-29	Elongation Factor G, domain III
EFG_IV	PF03764	530 - 650	6.2e-38	Elongation factor G, domain IV
EFG_C	PF00679	654 - 739	2e-25	Elongation factor G C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0012306)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G45332 AT1G45332 AT2G45030
fragaria_vesca	FvH4_7g10500 FvH4_7g10500 FvH4_7g10500
malus_domestica	MD07G1111000.v1.1
prunus_persica	Prupe.2G134500_v2.0.a1
pyrus_communis	pycom07g09580
rosa_chinensis	RchiOBHm_Chr1g0348291
rosa_laevigata	RLG00000028669
rosa_multiflora	Rmu_sc0027364.1_g000002
rosa_roxburghii	Rroxscaffold_4G00307040
rosa_rugosa	Rorug01G0194100
rosa_samantha	Rh1AG211300 Rh1BG177800 Rh1CG195700 Rh1DG207300
rosa_wichuraiana	Rw1G017850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	566
AatII	GACGTC	1 cut(s)	2132
AccB1I	GGYRCC	1 cut(s)	305
AccB7I	CCANNNNNTGG	1 cut(s)	1882
AccBSI	CCGCTC	1 cut(s)	1491
AccII	CGCG	1 cut(s)	788
AclWI	GGATC	4 cut(s)	259, 587, 1451, 1720
AcoI	YGGCCR	2 cut(s)	177, 1828
AcsI	RAATTY	6 cut(s)	243, 920, 1730, 1768, 2003, 2162
AcuI	CTGAAG	3 cut(s)	864, 1032, 1585
AcvI	CACGTG	1 cut(s)	137
AcyI	GRCGYC	4 cut(s)	65, 300, 306, 2129
AfaI	GTAC	5 cut(s)	501, 966, 987, 1995, 2177
AfiI	CCNNNNNNNGG	4 cut(s)	915, 1042, 1490, 1882
AflIII	ACRYGT	1 cut(s)	2112
AjiI	CACGTC	1 cut(s)	172
AjnI	CCWGG	2 cut(s)	179, 412
AjuI	GAANNNNNNNTTGG	2 cut(s)	1107, 1139
Alw21I	GWGCWC	1 cut(s)	2187
Alw26I	GTCTC	3 cut(s)	288, 1601, 2136
AlwI	GGATC	4 cut(s)	259, 587, 1451, 1720
AoxI	GGCC	6 cut(s)	177, 204, 289, 1098, 1828, 2091
ApeKI	GCWGC	7 cut(s)	362, 641, 644, 1522, 1702, 1805, 1913
ApoI	RAATTY	6 cut(s)	243, 920, 1730, 1768, 2003, 2162
AseI	ATTAAT	1 cut(s)	1823
Asp700I	GAANNNNTTC	4 cut(s)	759, 849, 1548, 1845
AspLEI	GCGC	2 cut(s)	308, 2215
AspS9I	GGNCC	5 cut(s)	205, 1082, 1480, 2092, 2237
AsuHPI	GGTGA	5 cut(s)	226, 421, 1184, 1969, 2036
AsuII	TTCGAA	1 cut(s)	1427
AvaII	GGWCC	3 cut(s)	1082, 1480, 2237
BaeGI	GKGCMC	2 cut(s)	867, 2015
BalI	TGGCCA	2 cut(s)	179, 1830
BanI	GGYRCC	1 cut(s)	305
BanII	GRGCYC	1 cut(s)	1820
BarI	GAAGNNNNNNTAC	2 cut(s)	1146, 1178
BauI	CACGAG	2 cut(s)	269, 1590
BbrPI	CACGTG	1 cut(s)	137
BbsI	GAAGAC	1 cut(s)	762
Bbv12I	GWGCWC	1 cut(s)	2187
BbvI	GCAGC	7 cut(s)	349, 628, 631, 1509, 1689, 1817, 1900
BceAI	ACGGC	3 cut(s)	52, 313, 2106
BcgI	CGANNNNNNTGC	2 cut(s)	1794, 1828
BciT130I	CCWGG	2 cut(s)	181, 414
BclI	TGATCA	1 cut(s)	2062
BcoDI	GTCTC	3 cut(s)	288, 1601, 2136
BfaI	CTAG	2 cut(s)	732, 1890
BfmI	CTRYAG	2 cut(s)	1707, 1998
BfoI	RGCGCY	1 cut(s)	309
BglI	GCCNNNNNGGC	1 cut(s)	297
BisI	GCNGC	7 cut(s)	363, 642, 645, 1523, 1703, 1806, 1914
BlsI	GCNGC	7 cut(s)	364, 643, 646, 1524, 1704, 1807, 1915
Bme1390I	CCNGG	2 cut(s)	181, 414
Bme18I	GGWCC	3 cut(s)	1082, 1480, 2237
BmgBI	CACGTC	1 cut(s)	172
BmgT120I	GGNCC	5 cut(s)	205, 1082, 1480, 2092, 2237
BmrFI	CCNGG	2 cut(s)	181, 414
BmsI	GCATC	4 cut(s)	643, 1221, 1316, 1561
BpiI	GAAGAC	1 cut(s)	762
BpmI	CTGGAG	4 cut(s)	905, 1059, 1677, 1985
Bpu10I	CCTNAGC	1 cut(s)	1368
Bpu14I	TTCGAA	1 cut(s)	1427
BsaAI	YACGTR	1 cut(s)	137
BsaHI	GRCGYC	4 cut(s)	65, 300, 306, 2129
BsaJI	CCNNGG	4 cut(s)	141, 321, 412, 596
BsaWI	WCCGGW	1 cut(s)	257
BsaXI	ACNNNNNCTCC	2 cut(s)	146, 176
Bsc4I	CCNNNNNNNGG	4 cut(s)	915, 1042, 1490, 1882
Bse118I	RCCGGY	2 cut(s)	122, 1954
Bse1I	ACTGG	6 cut(s)	1201, 1373, 1397, 1690, 1835, 1968
Bse3DI	GCAATG	1 cut(s)	647
BseBI	CCWGG	2 cut(s)	181, 414
BseDI	CCNNGG	4 cut(s)	141, 321, 412, 596
BseGI	GGATG	5 cut(s)	471, 1051, 1236, 1831, 2080
BseLI	CCNNNNNNNGG	4 cut(s)	915, 1042, 1490, 1882
BseMI	GCAATG	1 cut(s)	647
BseMII	CTCAG	2 cut(s)	1359, 1476
BseNI	ACTGG	6 cut(s)	1201, 1373, 1397, 1690, 1835, 1968
BseRI	GAGGAG	3 cut(s)	26, 70, 77
BseSI	GKGCMC	2 cut(s)	867, 2015
BseXI	GCAGC	7 cut(s)	349, 628, 631, 1509, 1689, 1817, 1900
BseYI	CCCAGC	1 cut(s)	1777
BsgI	GTGCAG	3 cut(s)	358, 722, 2228
Bsh1236I	CGCG	1 cut(s)	788
BshFI	GGCC	6 cut(s)	179, 206, 291, 1100, 1830, 2093
BshNI	GGYRCC	1 cut(s)	305
BsiHKAI	GWGCWC	1 cut(s)	2187
BsiSI	CCGG	5 cut(s)	123, 219, 258, 631, 1955
BslFI	GGGAC	5 cut(s)	55, 1349, 1764, 2084, 2250
BslI	CCNNNNNNNGG	4 cut(s)	915, 1042, 1490, 1882
BsmAI	GTCTC	3 cut(s)	288, 1601, 2136
BsmBI	CGTCTC	2 cut(s)	288, 2136
BsmFI	GGGAC	5 cut(s)	55, 1349, 1764, 2084, 2250
BsmI	GAATGC	2 cut(s)	1234, 1548
BsnI	GGCC	6 cut(s)	179, 206, 291, 1100, 1830, 2093
Bsp119I	TTCGAA	1 cut(s)	1427
Bsp1286I	GDGCHC	4 cut(s)	867, 1820, 2015, 2187
Bsp1407I	TGTACA	2 cut(s)	499, 964
Bsp19I	CCATGG	2 cut(s)	321, 596
BspANI	GGCC	6 cut(s)	179, 206, 291, 1100, 1830, 2093
BspCNI	CTCAG	2 cut(s)	1360, 1477
BspFNI	CGCG	1 cut(s)	788
BspHI	TCATGA	2 cut(s)	277, 2200
BspMAI	CTGCAG	1 cut(s)	1711
BspPI	GGATC	4 cut(s)	259, 587, 1451, 1720
BspT104I	TTCGAA	1 cut(s)	1427
BspT107I	GGYRCC	1 cut(s)	305
BsrBI	CCGCTC	1 cut(s)	1491
BsrDI	GCAATG	1 cut(s)	647
BsrFI	RCCGGY	2 cut(s)	122, 1954
BsrGI	TGTACA	2 cut(s)	499, 964
BsrI	ACTGG	6 cut(s)	1201, 1373, 1397, 1690, 1835, 1968
BssAI	RCCGGY	2 cut(s)	122, 1954
BssECI	CCNNGG	4 cut(s)	141, 321, 412, 596
BssNI	GRCGYC	4 cut(s)	65, 300, 306, 2129
BssSI	CACGAG	2 cut(s)	269, 1590
BssT1I	CCWWGG	3 cut(s)	141, 321, 596
Bst2BI	CACGAG	2 cut(s)	269, 1590
Bst2UI	CCWGG	2 cut(s)	181, 414
Bst4CI	ACNGT	8 cut(s)	386, 520, 1331, 1579, 1612, 1742, 1945, 2017
Bst6I	CTCTTC	2 cut(s)	1044, 2139
BstACI	GRCGYC	4 cut(s)	65, 300, 306, 2129
BstAPI	GCANNNNNTGC	1 cut(s)	1922
BstAUI	TGTACA	2 cut(s)	499, 964
BstBAI	YACGTR	1 cut(s)	137
BstBI	TTCGAA	1 cut(s)	1427
BstC8I	GCNNGC	3 cut(s)	124, 1707, 2211
BstDEI	CTNAG	5 cut(s)	624, 1041, 1142, 1368, 1485
BstDSI	CCRYGG	2 cut(s)	321, 596
BstF5I	GGATG	5 cut(s)	471, 1051, 1236, 1831, 2080
BstFNI	CGCG	1 cut(s)	788
BstH2I	RGCGCY	1 cut(s)	309
BstHHI	GCGC	2 cut(s)	308, 2215
BstMAI	GTCTC	3 cut(s)	288, 1601, 2136
BstMWI	GCNNNNNNNGC	7 cut(s)	15, 297, 785, 911, 1715, 1877, 1922
BstNI	CCWGG	2 cut(s)	181, 414
BstNSI	RCATGY	2 cut(s)	1271, 2116
BstSCI	CCNGG	2 cut(s)	179, 412
BstSFI	CTRYAG	2 cut(s)	1707, 1998
BstSLI	GKGCMC	2 cut(s)	867, 2015
BstUI	CGCG	1 cut(s)	788
BstV1I	GCAGC	7 cut(s)	349, 628, 631, 1509, 1689, 1817, 1900
BstV2I	GAAGAC	1 cut(s)	762
BstX2I	RGATCY	3 cut(s)	264, 592, 1456
BstXI	CCANNNNNNTGG	1 cut(s)	328
BstYI	RGATCY	3 cut(s)	264, 592, 1456
BsuRI	GGCC	6 cut(s)	179, 206, 291, 1100, 1830, 2093
BtgI	CCRYGG	2 cut(s)	321, 596
BtgZI	GCGATG	1 cut(s)	895
BtrI	CACGTC	1 cut(s)	172
BtsCI	GGATG	5 cut(s)	471, 1051, 1236, 1831, 2080
BtsIMutI	CAGTG	4 cut(s)	1194, 1380, 2013, 2247
Cac8I	GCNNGC	3 cut(s)	124, 1707, 2211
CciI	TCATGA	2 cut(s)	277, 2200
CfoI	GCGC	2 cut(s)	308, 2215
Cfr10I	RCCGGY	2 cut(s)	122, 1954
Cfr13I	GGNCC	5 cut(s)	205, 1082, 1480, 2092, 2237
CseI	GACGC	2 cut(s)	73, 289
Csp6I	GTAC	5 cut(s)	500, 965, 986, 1994, 2176
CviQI	GTAC	5 cut(s)	500, 965, 986, 1994, 2176
DdeI	CTNAG	5 cut(s)	624, 1041, 1142, 1368, 1485
DinI	GGCGCC	1 cut(s)	307
DraI	TTTAAA	3 cut(s)	952, 1105, 1798
EaeI	YGGCCR	2 cut(s)	177, 1828
Eam1104I	CTCTTC	2 cut(s)	1044, 2139
EarI	CTCTTC	2 cut(s)	1044, 2139
EciI	GGCGGA	2 cut(s)	98, 109
Eco130I	CCWWGG	3 cut(s)	141, 321, 596
Eco24I	GRGCYC	1 cut(s)	1820
Eco47I	GGWCC	3 cut(s)	1082, 1480, 2237
Eco57I	CTGAAG	3 cut(s)	864, 1032, 1585
Eco72I	CACGTG	1 cut(s)	137
EcoO109I	RGGNCCY	1 cut(s)	2237
EcoRI	GAATTC	2 cut(s)	243, 2162
EcoRII	CCWGG	2 cut(s)	179, 412
EcoT14I	CCWWGG	3 cut(s)	141, 321, 596
EcoT22I	ATGCAT	1 cut(s)	1236
EcoT38I	GRGCYC	1 cut(s)	1820
EgeI	GGCGCC	1 cut(s)	307
EheI	GGCGCC	1 cut(s)	307
ErhI	CCWWGG	3 cut(s)	141, 321, 596
Esp3I	CGTCTC	2 cut(s)	288, 2136
FalI	AAGNNNNNCTT	2 cut(s)	837, 869
FaqI	GGGAC	5 cut(s)	55, 1349, 1764, 2084, 2250
FauI	CCCGC	2 cut(s)	14, 23
FbaI	TGATCA	1 cut(s)	2062
Fnu4HI	GCNGC	7 cut(s)	363, 642, 645, 1523, 1703, 1806, 1914
FokI	GGATG	5 cut(s)	458, 1058, 1243, 1818, 2087
FriOI	GRGCYC	1 cut(s)	1820
Fsp4HI	GCNGC	7 cut(s)	363, 642, 645, 1523, 1703, 1806, 1914
FspBI	CTAG	2 cut(s)	732, 1890
GlaI	GCGC	2 cut(s)	307, 2214
GluI	GCNGC	7 cut(s)	363, 642, 645, 1523, 1703, 1806, 1914
GsaI	CCCAGC	1 cut(s)	1781
GsuI	CTGGAG	4 cut(s)	905, 1059, 1677, 1985
HaeII	RGCGCY	1 cut(s)	309
HaeIII	GGCC	6 cut(s)	179, 206, 291, 1100, 1830, 2093
HapII	CCGG	5 cut(s)	123, 219, 258, 631, 1955
HgaI	GACGC	2 cut(s)	73, 289
HhaI	GCGC	2 cut(s)	308, 2215
Hin1I	GRCGYC	4 cut(s)	65, 300, 306, 2129
Hin6I	GCGC	2 cut(s)	306, 2213
HinP1I	GCGC	2 cut(s)	306, 2213
HincII	GTYRAC	1 cut(s)	1134
HindII	GTYRAC	1 cut(s)	1134
HindIII	AAGCTT	1 cut(s)	711
HpaI	GTTAAC	1 cut(s)	1134
HpaII	CCGG	5 cut(s)	123, 219, 258, 631, 1955
HphI	GGTGA	5 cut(s)	226, 421, 1184, 1969, 2036
Hpy166II	GTNNAC	8 cut(s)	165, 500, 965, 986, 1134, 1597, 1895, 2224
Hpy8I	GTNNAC	8 cut(s)	165, 500, 965, 986, 1134, 1597, 1895, 2224
Hpy99I	CGWCG	4 cut(s)	67, 95, 305, 2131
HpyCH4III	ACNGT	8 cut(s)	386, 520, 1331, 1579, 1612, 1742, 1945, 2017
HpyCH4IV	ACGT	5 cut(s)	136, 171, 420, 1621, 2129
HpyF10VI	GCNNNNNNNGC	7 cut(s)	15, 297, 785, 911, 1715, 1877, 1922
HpyF3I	CTNAG	5 cut(s)	624, 1041, 1142, 1368, 1485
HpySE526I	ACGT	5 cut(s)	136, 171, 420, 1621, 2129
Hsp92I	GRCGYC	4 cut(s)	65, 300, 306, 2129
HspAI	GCGC	2 cut(s)	306, 2213
KasI	GGCGCC	1 cut(s)	305
KflI	GGGWCCC	1 cut(s)	2237
KroI	GCCGGC	1 cut(s)	122
KroNI	GCCGGC	1 cut(s)	124
Ksp22I	TGATCA	1 cut(s)	2062
KspAI	GTTAAC	1 cut(s)	1134
LmnI	GCTCC	7 cut(s)	101, 154, 189, 467, 587, 633, 1966
Lsp1109I	GCAGC	7 cut(s)	349, 628, 631, 1509, 1689, 1817, 1900
LweI	GCATC	4 cut(s)	643, 1221, 1316, 1561
MaeI	CTAG	2 cut(s)	732, 1890
MaeII	ACGT	5 cut(s)	136, 171, 420, 1621, 2129
MaeIII	GTNAC	4 cut(s)	416, 1019, 1612, 1681
MbiI	CCGCTC	1 cut(s)	1491
MboII	GAAGA	7 cut(s)	683, 767, 869, 1061, 1216, 1466, 2126
MflI	RGATCY	3 cut(s)	264, 592, 1456
MhlI	GDGCHC	4 cut(s)	867, 1820, 2015, 2187
MlsI	TGGCCA	2 cut(s)	179, 1830
MluNI	TGGCCA	2 cut(s)	179, 1830
Mly113I	GGCGCC	1 cut(s)	306
MlyI	GAGTC	4 cut(s)	209, 311, 514, 2072
MmeI	TCCRAC	2 cut(s)	648, 1561
Mox20I	TGGCCA	2 cut(s)	179, 1830
Mph1103I	ATGCAT	1 cut(s)	1236
MroNI	GCCGGC	1 cut(s)	122
MroXI	GAANNNNTTC	4 cut(s)	759, 849, 1548, 1845
MscI	TGGCCA	2 cut(s)	179, 1830
MslI	CAYNNNNRTG	1 cut(s)	1239
Msp20I	TGGCCA	2 cut(s)	179, 1830
MspA1I	CMGCKG	2 cut(s)	362, 1702
MspI	CCGG	5 cut(s)	123, 219, 258, 631, 1955
MspR9I	CCNGG	2 cut(s)	181, 414
Mva1269I	GAATGC	2 cut(s)	1234, 1548
MvaI	CCWGG	2 cut(s)	181, 414
MvnI	CGCG	1 cut(s)	788
MwoI	GCNNNNNNNGC	7 cut(s)	15, 297, 785, 911, 1715, 1877, 1922
NaeI	GCCGGC	1 cut(s)	124
NarI	GGCGCC	1 cut(s)	306
NcoI	CCATGG	2 cut(s)	321, 596
NgoMIV	GCCGGC	1 cut(s)	122
NmeAIII	GCCGAG	2 cut(s)	182, 317
NmuCI	GTSAC	1 cut(s)	416
NsiI	ATGCAT	1 cut(s)	1236
NspI	RCATGY	2 cut(s)	1271, 2116
NspV	TTCGAA	1 cut(s)	1427
PagI	TCATGA	2 cut(s)	277, 2200
PciI	ACATGT	1 cut(s)	2112
PcsI	WCGNNNNNNNCGW	1 cut(s)	71
PctI	GAATGC	2 cut(s)	1234, 1548
PdiI	GCCGGC	1 cut(s)	124
PdmI	GAANNNNTTC	4 cut(s)	759, 849, 1548, 1845
PfeI	GAWTC	7 cut(s)	198, 274, 610, 1146, 1409, 1438, 1886
PflFI	GACNNNGTC	3 cut(s)	67, 299, 1665
PflMI	CCANNNNNTGG	1 cut(s)	1882
PkrI	GCNGC	7 cut(s)	364, 643, 646, 1524, 1704, 1807, 1915
PleI	GAGTC	4 cut(s)	209, 311, 513, 2072
PluTI	GGCGCC	1 cut(s)	309
PmaCI	CACGTG	1 cut(s)	137
PmlI	CACGTG	1 cut(s)	137
PpsI	GAGTC	4 cut(s)	209, 311, 513, 2072
Ppu21I	YACGTR	1 cut(s)	137
PpuMI	RGGWCCY	1 cut(s)	2237
PscI	ACATGT	1 cut(s)	2112
PshBI	ATTAAT	1 cut(s)	1823
PsiI	TTATAA	1 cut(s)	566
Psp5II	RGGWCCY	1 cut(s)	2237
Psp6I	CCWGG	2 cut(s)	179, 412
PspCI	CACGTG	1 cut(s)	137
PspFI	CCCAGC	1 cut(s)	1777
PspGI	CCWGG	2 cut(s)	179, 412
PspPI	GGNCC	5 cut(s)	205, 1082, 1480, 2092, 2237
PspPPI	RGGWCCY	1 cut(s)	2237
PstI	CTGCAG	1 cut(s)	1711
PsuI	RGATCY	3 cut(s)	264, 592, 1456
PsyI	GACNNNGTC	3 cut(s)	67, 299, 1665
RsaI	GTAC	5 cut(s)	501, 966, 987, 1995, 2177
RsaNI	GTAC	5 cut(s)	500, 965, 986, 1994, 2176
RseI	CAYNNNNRTG	1 cut(s)	1239
SatI	GCNGC	7 cut(s)	363, 642, 645, 1523, 1703, 1806, 1914
Sau96I	GGNCC	5 cut(s)	205, 1082, 1480, 2092, 2237
SbfI	CCTGCAGG	1 cut(s)	1711
SchI	GAGTC	4 cut(s)	209, 311, 514, 2072
ScrFI	CCNGG	2 cut(s)	181, 414
SdaI	CCTGCAGG	1 cut(s)	1711
SduI	GDGCHC	4 cut(s)	867, 1820, 2015, 2187
SfaNI	GCATC	4 cut(s)	643, 1221, 1316, 1561
SfcI	CTRYAG	2 cut(s)	1707, 1998
SfoI	GGCGCC	1 cut(s)	307
SfuI	TTCGAA	1 cut(s)	1427
SinI	GGWCC	3 cut(s)	1082, 1480, 2237
SmiMI	CAYNNNNRTG	1 cut(s)	1239
Sse8387I	CCTGCAGG	1 cut(s)	1711
SspDI	GGCGCC	1 cut(s)	305
SspMI	CTAG	2 cut(s)	732, 1890
StyD4I	CCNGG	2 cut(s)	179, 412
StyI	CCWWGG	3 cut(s)	141, 321, 596
TaaI	ACNGT	8 cut(s)	386, 520, 1331, 1579, 1612, 1742, 1945, 2017
TaiI	ACGT	5 cut(s)	139, 174, 423, 1624, 2132
TaqI	TCGA	5 cut(s)	213, 580, 1427, 2126, 2137
TatI	WGTACW	4 cut(s)	499, 964, 985, 2175
TfiI	GAWTC	7 cut(s)	198, 274, 610, 1146, 1409, 1438, 1886
TscAI	CASTG	4 cut(s)	1201, 1380, 2020, 2254
TseFI	GTSAC	1 cut(s)	416
TseI	GCWGC	7 cut(s)	362, 641, 644, 1522, 1702, 1805, 1913
Tsp45I	GTSAC	1 cut(s)	416
TspDTI	ATGAA	8 cut(s)	266, 548, 713, 925, 1062, 1167, 1168, 1373
TspGWI	ACGGA	3 cut(s)	182, 1180, 1457
TspRI	CASTG	4 cut(s)	1201, 1380, 2020, 2254
Tth111I	GACNNNGTC	3 cut(s)	67, 299, 1665
Van91I	CCANNNNNTGG	1 cut(s)	1882
VpaK11BI	GGWCC	3 cut(s)	1082, 1480, 2237
VspI	ATTAAT	1 cut(s)	1823
XapI	RAATTY	6 cut(s)	243, 920, 1730, 1768, 2003, 2162
XceI	RCATGY	2 cut(s)	1271, 2116
XmnI	GAANNNNTTC	4 cut(s)	759, 849, 1548, 1845
XspI	CTAG	2 cut(s)	732, 1890
ZraI	GACGTC	1 cut(s)	2130
Zsp2I	ATGCAT	1 cut(s)	1236

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.