FvH4_7g12030

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0016387
Physical Location & Seq
Forward (+)
10939695 .. 10940585
891 bp
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UTR
Exon/CDS
Intron
FvH4_7g12030.t1

Sequence Viewer

Length: 588 bp
ATGAGTTGTTTTAGGATTTGCTTGACGGCTCTCCGGCGGTGCACGTTTAGTGGATGCAAGAGCTTTCGAAAGAAGAGAAGAAGGTCTTCCAACTTGGATGCACCGGCGACTGCTGCATCGTCTTTTGCGGCCATGGAAGTGTCAAATCAGTTCAAGCAAGTGTTTGAGGTGATGGACGCGAACGGGGACGGGAAGATTTCTCCGGTGGAGCTCAGTGAAGTTCTCTGCTGCCTCGGGTACAAGAACAAGTCGGCGGCTGCGAAAGAAGCCGAAGGGATGGTGAGAGAGATGGACTGCAATGGAGACGGGTTCATTGACTTGGATGAGTTCATGACTGCTGTGAGTGCTAACGATACAAAGCGTAGTGGTAACGATGGGGGTGATGATCTCATGGATGCTTTTCTTATATTTGATACTGATAAGAATGGGAAGATTTCCGCCAAGGAGTTGAGGAGGGTGCTGGTCAGCCTTGGATGTGAGAGAATCAGTCTCAAAGAGTGTAAGCAAATGATCAAAGGGGTTGATAGGAATGGGGATGGGGTTGTGGATTTTGAAGAATTTAGGTTGATGATGACCAGAAATGTGTAA

Protein Analysis

196

Amino Acids

21.57

Weight (kDa)

6.39

Isoelectric Point (pI)

44.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 50 - 114 3.8e-11 EF-hand domain pair
EF-hand_5 PF13202 51 - 74 4e-06 EF hand
EF-hand_1 PF00036 91 - 115 4e-06 EF hand domain
EF-hand_1 PF00036 129 - 156 1.7e-06 EF hand domain
EF-hand_6 PF13405 129 - 158 5.1e-08 EF-hand domain
EF-hand_7 PF13499 129 - 191 4.9e-12 EF-hand domain pair
EF-hand_5 PF13202 131 - 154 1e-06 EF hand
EF-hand_8 PF13833 142 - 194 5.2e-10 EF-hand domain pair
EF-hand_1 PF00036 166 - 193 9.7e-08 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016387)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g12030
malus_domestica MD02G1202000.v1.1 MD07G1123300.v1.1
prunus_persica Prupe.2G146900_v2.0.a1
pyrus_communis pycom07g11200
rosa_chinensis RchiOBHm_Chr1g0350741
rosa_laevigata RLG00000028458
rosa_multiflora Rmu_sc0007810.1_g000002
rosa_roxburghii Rroxscaffold_4G00304540
rosa_rugosa Rorug01G0213700
rosa_samantha Rh1AG227900 Rh1BG197400 Rh1CG212800 Rh1DG225300
rosa_wichuraiana Rw1G019740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 179
AciI CCGC 4 cut(s) 37, 128, 254, 438
AcoI YGGCCR 1 cut(s) 129
AcsI RAATTY 1 cut(s) 557
AfaI GTAC 1 cut(s) 239
AgsI TTSAA 2 cut(s) 154, 554
AluBI AGCT 2 cut(s) 63, 211
AluI AGCT 2 cut(s) 63, 211
Alw21I GWGCWC 2 cut(s) 44, 213
Alw26I GTCTC 2 cut(s) 297, 494
Alw44I GTGCAC 1 cut(s) 40
Ama87I CYCGRG 1 cut(s) 233
AoxI GGCC 1 cut(s) 129
ApaLI GTGCAC 1 cut(s) 40
ApeKI GCWGC 3 cut(s) 113, 228, 257
ApoI RAATTY 1 cut(s) 557
Asp700I GAANNNNTTC 1 cut(s) 85
AsuHPI GGTGA 3 cut(s) 181, 292, 392
AsuII TTCGAA 1 cut(s) 67
AvaI CYCGRG 1 cut(s) 233
BaeGI GKGCMC 1 cut(s) 44
BanII GRGCYC 1 cut(s) 213
BbsI GAAGAC 1 cut(s) 78
Bbv12I GWGCWC 2 cut(s) 44, 213
BbvI GCAGC 3 cut(s) 100, 215, 244
BccI CCATC 5 cut(s) 166, 271, 283, 368, 530
BceAI ACGGC 1 cut(s) 42
BclI TGATCA 1 cut(s) 510
BcoDI GTCTC 2 cut(s) 297, 494
BisI GCNGC 5 cut(s) 114, 129, 229, 255, 258
BlsI GCNGC 5 cut(s) 115, 130, 230, 256, 259
BmeT110I CYCGRG 1 cut(s) 233
BmsI GCATC 4 cut(s) 44, 88, 125, 385
BpiI GAAGAC 1 cut(s) 78
Bpu14I TTCGAA 1 cut(s) 67
BsaJI CCNNGG 4 cut(s) 132, 232, 441, 469
BsaWI WCCGGW 1 cut(s) 202
Bse118I RCCGGY 1 cut(s) 103
Bse3DI GCAATG 1 cut(s) 304
BseDI CCNNGG 4 cut(s) 132, 232, 441, 469
BseGI GGATG 7 cut(s) 59, 103, 282, 328, 400, 479, 541
BseMI GCAATG 1 cut(s) 304
BseMII CTCAG 1 cut(s) 226
BseRI GAGGAG 1 cut(s) 466
BseSI GKGCMC 1 cut(s) 44
BseXI GCAGC 3 cut(s) 100, 215, 244
Bsh1236I CGCG 1 cut(s) 179
BshFI GGCC 1 cut(s) 131
BsiHKAI GWGCWC 2 cut(s) 44, 213
BsiHKCI CYCGRG 1 cut(s) 233
BsiSI CCGG 3 cut(s) 34, 104, 203
BslFI GGGAC 1 cut(s) 200
BsmAI GTCTC 2 cut(s) 297, 494
BsmBI CGTCTC 1 cut(s) 297
BsmFI GGGAC 1 cut(s) 200
BsnI GGCC 1 cut(s) 131
BsoBI CYCGRG 1 cut(s) 233
Bsp119I TTCGAA 1 cut(s) 67
Bsp1286I GDGCHC 2 cut(s) 44, 213
Bsp143I GATC 2 cut(s) 385, 510
Bsp19I CCATGG 1 cut(s) 132
BspACI CCGC 4 cut(s) 37, 128, 254, 438
BspANI GGCC 1 cut(s) 131
BspCNI CTCAG 1 cut(s) 225
BspFNI CGCG 1 cut(s) 179
BspHI TCATGA 1 cut(s) 330
BspT104I TTCGAA 1 cut(s) 67
BsrDI GCAATG 1 cut(s) 304
BsrFI RCCGGY 1 cut(s) 103
BssAI RCCGGY 1 cut(s) 103
BssECI CCNNGG 4 cut(s) 132, 232, 441, 469
BssMI GATC 2 cut(s) 385, 510
BssT1I CCWWGG 3 cut(s) 132, 441, 469
Bst6I CTCTTC 1 cut(s) 68
BstBI TTCGAA 1 cut(s) 67
BstDEI CTNAG 1 cut(s) 212
BstDSI CCRYGG 1 cut(s) 132
BstF5I GGATG 7 cut(s) 59, 103, 282, 328, 400, 479, 541
BstFNI CGCG 1 cut(s) 179
BstKTI GATC 2 cut(s) 388, 513
BstMAI GTCTC 2 cut(s) 297, 494
BstMBI GATC 2 cut(s) 385, 510
BstMWI GCNNNNNNNGC 3 cut(s) 113, 266, 344
BstSLI GKGCMC 1 cut(s) 44
BstUI CGCG 1 cut(s) 179
BstV1I GCAGC 3 cut(s) 100, 215, 244
BstV2I GAAGAC 1 cut(s) 78
BsuRI GGCC 1 cut(s) 131
BtgI CCRYGG 1 cut(s) 132
BtsCI GGATG 7 cut(s) 59, 103, 282, 328, 400, 479, 541
BtsIMutI CAGTG 1 cut(s) 220
CciI TCATGA 1 cut(s) 330
Cfr10I RCCGGY 1 cut(s) 103
CseI GACGC 1 cut(s) 185
Csp6I GTAC 1 cut(s) 238
CviAII CATG 3 cut(s) 133, 331, 391
CviJI RGCY 7 cut(s) 29, 63, 131, 211, 257, 269, 468
CviKI_1 RGCY 7 cut(s) 29, 63, 131, 211, 257, 269, 468
CviQI GTAC 1 cut(s) 238
DdeI CTNAG 1 cut(s) 212
DpnI GATC 2 cut(s) 387, 512
DpnII GATC 2 cut(s) 385, 510
EaeI YGGCCR 1 cut(s) 129
Eam1104I CTCTTC 1 cut(s) 68
EarI CTCTTC 1 cut(s) 68
EciI GGCGGA 1 cut(s) 427
Ecl136II GAGCTC 1 cut(s) 211
Eco130I CCWWGG 3 cut(s) 132, 441, 469
Eco24I GRGCYC 1 cut(s) 213
Eco53kI GAGCTC 1 cut(s) 211
Eco88I CYCGRG 1 cut(s) 233
EcoICRI GAGCTC 1 cut(s) 211
EcoT14I CCWWGG 3 cut(s) 132, 441, 469
EcoT38I GRGCYC 1 cut(s) 213
ErhI CCWWGG 3 cut(s) 132, 441, 469
Esp3I CGTCTC 1 cut(s) 297
FaeI CATG 3 cut(s) 136, 334, 394
FaiI YATR 4 cut(s) 134, 332, 392, 407
FalI AAGNNNNNCTT 2 cut(s) 70, 102
FaqI GGGAC 1 cut(s) 200
FatI CATG 3 cut(s) 132, 330, 390
FbaI TGATCA 1 cut(s) 510
Fnu4HI GCNGC 5 cut(s) 114, 129, 229, 255, 258
FokI GGATG 7 cut(s) 66, 110, 289, 335, 407, 486, 548
FriOI GRGCYC 1 cut(s) 213
Fsp4HI GCNGC 5 cut(s) 114, 129, 229, 255, 258
GluI GCNGC 5 cut(s) 114, 129, 229, 255, 258
HaeIII GGCC 1 cut(s) 131
HapII CCGG 3 cut(s) 34, 104, 203
HgaI GACGC 1 cut(s) 185
Hin1II CATG 3 cut(s) 136, 334, 394
HinfI GANTC 1 cut(s) 483
HpaII CCGG 3 cut(s) 34, 104, 203
HphI GGTGA 3 cut(s) 181, 292, 392
Hpy166II GTNNAC 1 cut(s) 42
Hpy188III TCNNGA 1 cut(s) 331
Hpy8I GTNNAC 1 cut(s) 42
HpyAV CCTTC 2 cut(s) 75, 266
HpyCH4IV ACGT 1 cut(s) 44
HpyCH4V TGCA 5 cut(s) 42, 57, 101, 116, 297
HpyF10VI GCNNNNNNNGC 3 cut(s) 113, 266, 344
HpyF3I CTNAG 1 cut(s) 212
HpySE526I ACGT 1 cut(s) 44
Hsp92II CATG 3 cut(s) 136, 334, 394
Ksp22I TGATCA 1 cut(s) 510
Kzo9I GATC 2 cut(s) 385, 510
LmnI GCTCC 1 cut(s) 208
LpnPI CCDG 4 cut(s) 47, 117, 216, 446
Lsp1109I GCAGC 3 cut(s) 100, 215, 244
LweI GCATC 4 cut(s) 44, 88, 125, 385
MaeII ACGT 1 cut(s) 44
MaeIII GTNAC 1 cut(s) 368
MalI GATC 2 cut(s) 387, 512
MboI GATC 2 cut(s) 385, 510
MboII GAAGA 6 cut(s) 78, 85, 90, 205, 442, 566
MhlI GDGCHC 2 cut(s) 44, 213
MluCI AATT 1 cut(s) 557
MmeI TCCRAC 1 cut(s) 114
MnlI CCTC 4 cut(s) 160, 242, 444, 447
MroXI GAANNNNTTC 1 cut(s) 85
MslI CAYNNNNRTG 1 cut(s) 137
MspI CCGG 3 cut(s) 34, 104, 203
MvnI CGCG 1 cut(s) 179
MwoI GCNNNNNNNGC 3 cut(s) 113, 266, 344
NcoI CCATGG 1 cut(s) 132
NdeII GATC 2 cut(s) 385, 510
NlaIII CATG 3 cut(s) 136, 334, 394
NspV TTCGAA 1 cut(s) 67
PagI TCATGA 1 cut(s) 330
PcsI WCGNNNNNNNCGW 1 cut(s) 257
PdmI GAANNNNTTC 1 cut(s) 85
PfeI GAWTC 1 cut(s) 483
PkrI GCNGC 5 cut(s) 115, 130, 230, 256, 259
Psp124BI GAGCTC 1 cut(s) 213
RsaI GTAC 1 cut(s) 239
RsaNI GTAC 1 cut(s) 238
RseI CAYNNNNRTG 1 cut(s) 137
SacI GAGCTC 1 cut(s) 213
SatI GCNGC 5 cut(s) 114, 129, 229, 255, 258
Sau3AI GATC 2 cut(s) 385, 510
SduI GDGCHC 2 cut(s) 44, 213
SetI ASST 6 cut(s) 47, 65, 86, 171, 213, 566
SfaNI GCATC 4 cut(s) 44, 88, 125, 385
SfuI TTCGAA 1 cut(s) 67
SgrAI CRCCGGYG 1 cut(s) 103
SmiMI CAYNNNNRTG 1 cut(s) 137
Sse9I AATT 1 cut(s) 557
SsiI CCGC 4 cut(s) 37, 128, 254, 438
SstI GAGCTC 1 cut(s) 213
StyI CCWWGG 3 cut(s) 132, 441, 469
TaiI ACGT 1 cut(s) 47
TaqI TCGA 1 cut(s) 67
TasI AATT 1 cut(s) 557
TauI GCSGC 2 cut(s) 131, 257
TfiI GAWTC 1 cut(s) 483
TscAI CASTG 1 cut(s) 220
TseI GCWGC 3 cut(s) 113, 228, 257
TspDTI ATGAA 2 cut(s) 301, 319
TspRI CASTG 1 cut(s) 220
VneI GTGCAC 1 cut(s) 40
XapI RAATTY 1 cut(s) 557
XmnI GAANNNNTTC 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.