MD02G1202000.v1.1

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0016387
Physical Location & Seq
Reverse (-)
20017159 .. 20017776
618 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1202000.v1.1.491

Sequence Viewer

Length: 618 bp
ATGATGAGTTGTATCAGAATTTTCTTGGCGTCAACCGTCCAGAGGATGACAAGTGGATACAAGAAAAGAAGGACACCGAAACTTTCTTCCAGGTTTGATGTGCGGCCTGCGGCTGCAACCTCATCTTTTGCAGGCATGGAAGTCTCCAGCCAATTCAGGCAAGTGTTTGAGGTCATGGATGCAAATGGGGACGGGAAAATCTCCCCTCTCGAGCTCAGCGCCGTGCTTTCGTGCCTCGGAAACAAGAAGTCGATCGCCGCAGAGGAAGCTGAAGGGATTGTGAGGGAAATGGATCGTAACGGAGATGGATGCATAGACTTGGATGAATTCATGAACGCAGTGAATGTTGACAGATCAAGAAGTAATGATAGTGATAGTGTTGGGTTTGGAAATGAGGAAGAAGACTGTGAACTCATGGATGCTTTTCGTATTTTCGACACGGATAAGAACGGGAAGATTTCAGCCAAGGAGTTGCAGAGGGTTCTGGTCAGCCTTGGATGCAAAGAATGCAGCCTTAGAGAGTGTAGGCAAATGATTAAAGGGGTTGATAGGAATGGAGATGGAGCTGTGGATTTTGAAGAATTTAGGTTGATGATGACAAGAAACTATGCGTGCTGA

Protein Analysis

206

Amino Acids

22.82

Weight (kDa)

5.05

Isoelectric Point (pI)

45.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_6 PF13405 51 - 80 9e-06 EF-hand domain
EF-hand_7 PF13499 51 - 113 1.4e-13 EF-hand domain pair
EF-hand_5 PF13202 53 - 75 7.3e-07 EF hand
EF-hand_8 PF13833 64 - 114 7.2e-06 EF-hand domain pair
EF-hand_1 PF00036 137 - 164 1.5e-07 EF hand domain
EF-hand_6 PF13405 137 - 166 6.7e-09 EF-hand domain
EF-hand_7 PF13499 137 - 199 1.4e-12 EF-hand domain pair
EF-hand_5 PF13202 139 - 162 6.8e-08 EF hand
EF-hand_8 PF13833 150 - 202 8.7e-10 EF-hand domain pair
EF-hand_1 PF00036 174 - 201 3.1e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0016387)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g12030
malus_domestica MD02G1202000.v1.1 MD07G1123300.v1.1
prunus_persica Prupe.2G146900_v2.0.a1
pyrus_communis pycom07g11200
rosa_chinensis RchiOBHm_Chr1g0350741
rosa_laevigata RLG00000028458
rosa_multiflora Rmu_sc0007810.1_g000002
rosa_roxburghii Rroxscaffold_4G00304540
rosa_rugosa Rorug01G0213700
rosa_samantha Rh1AG227900 Rh1BG197400 Rh1CG212800 Rh1DG225300
rosa_wichuraiana Rw1G019740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 103, 110, 258
AclWI GGATC 1 cut(s) 300
AcsI RAATTY 3 cut(s) 18, 326, 581
AcuI CTGAAG 1 cut(s) 291
AcyI GRCGYC 1 cut(s) 29
AfiI CCNNNNNNNGG 1 cut(s) 42
AgsI TTSAA 1 cut(s) 578
AjnI CCWGG 1 cut(s) 89
AluBI AGCT 3 cut(s) 214, 269, 566
AluI AGCT 3 cut(s) 214, 269, 566
Alw21I GWGCWC 1 cut(s) 216
Alw26I GTCTC 1 cut(s) 148
AlwI GGATC 1 cut(s) 300
Ama87I CYCGRG 1 cut(s) 209
AoxI GGCC 1 cut(s) 104
ApeKI GCWGC 2 cut(s) 113, 510
ApoI RAATTY 3 cut(s) 18, 326, 581
AspLEI GCGC 1 cut(s) 221
AvaI CYCGRG 1 cut(s) 209
BanII GRGCYC 1 cut(s) 216
BbsI GAAGAC 1 cut(s) 408
Bbv12I GWGCWC 1 cut(s) 216
BbvI GCAGC 2 cut(s) 100, 522
BccI CCATC 2 cut(s) 299, 554
BceAI ACGGC 1 cut(s) 206
BciT130I CCWGG 1 cut(s) 91
BciVI GTATCC 1 cut(s) 50
BcoDI GTCTC 1 cut(s) 148
BfoI RGCGCY 1 cut(s) 222
BfuI GTATCC 1 cut(s) 50
BisI GCNGC 5 cut(s) 104, 111, 114, 258, 511
BlpI GCTNAGC 1 cut(s) 215
BlsI GCNGC 5 cut(s) 105, 112, 115, 259, 512
Bme1390I CCNGG 1 cut(s) 91
BmeT110I CYCGRG 1 cut(s) 209
BmrFI CCNGG 1 cut(s) 91
BmsI GCATC 4 cut(s) 169, 299, 409, 488
BpiI GAAGAC 1 cut(s) 408
BpmI CTGGAG 1 cut(s) 130
Bpu1102I GCTNAGC 1 cut(s) 215
BsaHI GRCGYC 1 cut(s) 29
BsaJI CCNNGG 3 cut(s) 235, 465, 493
Bsc4I CCNNNNNNNGG 1 cut(s) 42
BseBI CCWGG 1 cut(s) 91
BseDI CCNNGG 3 cut(s) 235, 465, 493
BseGI GGATG 6 cut(s) 51, 184, 314, 328, 424, 503
BseLI CCNNNNNNNGG 1 cut(s) 42
BseMII CTCAG 1 cut(s) 229
BseXI GCAGC 2 cut(s) 100, 522
Bsh1285I CGRYCG 1 cut(s) 255
BshFI GGCC 1 cut(s) 106
BsiEI CGRYCG 1 cut(s) 255
BsiHKAI GWGCWC 1 cut(s) 216
BsiHKCI CYCGRG 1 cut(s) 209
BslFI GGGAC 1 cut(s) 203
BslI CCNNNNNNNGG 1 cut(s) 42
BsmAI GTCTC 1 cut(s) 148
BsmFI GGGAC 1 cut(s) 203
BsmI GAATGC 1 cut(s) 512
BsnI GGCC 1 cut(s) 106
BsoBI CYCGRG 1 cut(s) 209
Bsp1286I GDGCHC 1 cut(s) 216
Bsp143I GATC 3 cut(s) 252, 292, 353
Bsp1720I GCTNAGC 1 cut(s) 215
BspACI CCGC 3 cut(s) 103, 110, 258
BspANI GGCC 1 cut(s) 106
BspCNI CTCAG 1 cut(s) 228
BspHI TCATGA 1 cut(s) 330
BspPI GGATC 1 cut(s) 300
BssECI CCNNGG 3 cut(s) 235, 465, 493
BssMI GATC 3 cut(s) 252, 292, 353
BssNI GRCGYC 1 cut(s) 29
BssT1I CCWWGG 2 cut(s) 465, 493
Bst2UI CCWGG 1 cut(s) 91
Bst4CI ACNGT 2 cut(s) 37, 407
BstACI GRCGYC 1 cut(s) 29
BstAPI GCANNNNNTGC 1 cut(s) 507
BstC8I GCNNGC 3 cut(s) 108, 133, 613
BstDEI CTNAG 2 cut(s) 215, 515
BstF5I GGATG 6 cut(s) 51, 184, 314, 328, 424, 503
BstH2I RGCGCY 1 cut(s) 222
BstHHI GCGC 1 cut(s) 221
BstKTI GATC 3 cut(s) 255, 295, 356
BstMAI GTCTC 1 cut(s) 148
BstMBI GATC 3 cut(s) 252, 292, 353
BstMCI CGRYCG 1 cut(s) 255
BstMWI GCNNNNNNNGC 3 cut(s) 266, 498, 507
BstNI CCWGG 1 cut(s) 91
BstSCI CCNGG 1 cut(s) 89
BstV1I GCAGC 2 cut(s) 100, 522
BstV2I GAAGAC 1 cut(s) 408
BsuI GTATCC 1 cut(s) 50
BsuRI GGCC 1 cut(s) 106
BtsCI GGATG 6 cut(s) 51, 184, 314, 328, 424, 503
BtsI GCAGTG 1 cut(s) 345
BtsIMutI CAGTG 1 cut(s) 345
Cac8I GCNNGC 3 cut(s) 108, 133, 613
CciI TCATGA 1 cut(s) 330
CfoI GCGC 1 cut(s) 221
CseI GACGC 1 cut(s) 18
CviAII CATG 4 cut(s) 136, 175, 331, 415
CviJI RGCY 9 cut(s) 106, 113, 150, 214, 269, 464, 492, 513, 566
CviKI_1 RGCY 9 cut(s) 106, 113, 150, 214, 269, 464, 492, 513, 566
DdeI CTNAG 2 cut(s) 215, 515
DpnI GATC 3 cut(s) 254, 294, 355
DpnII GATC 3 cut(s) 252, 292, 353
Ecl136II GAGCTC 1 cut(s) 214
Eco130I CCWWGG 2 cut(s) 465, 493
Eco24I GRGCYC 1 cut(s) 216
Eco53kI GAGCTC 1 cut(s) 214
Eco57I CTGAAG 1 cut(s) 291
Eco88I CYCGRG 1 cut(s) 209
EcoICRI GAGCTC 1 cut(s) 214
EcoRI GAATTC 1 cut(s) 326
EcoRII CCWGG 1 cut(s) 89
EcoT14I CCWWGG 2 cut(s) 465, 493
EcoT22I ATGCAT 1 cut(s) 314
EcoT38I GRGCYC 1 cut(s) 216
ErhI CCWWGG 2 cut(s) 465, 493
FaeI CATG 4 cut(s) 139, 178, 334, 418
FaiI YATR 6 cut(s) 137, 176, 314, 332, 416, 609
FaqI GGGAC 1 cut(s) 203
FatI CATG 4 cut(s) 135, 174, 330, 414
Fnu4HI GCNGC 5 cut(s) 104, 111, 114, 258, 511
FokI GGATG 6 cut(s) 58, 191, 321, 335, 431, 510
FriOI GRGCYC 1 cut(s) 216
Fsp4HI GCNGC 5 cut(s) 104, 111, 114, 258, 511
GlaI GCGC 1 cut(s) 220
GluI GCNGC 5 cut(s) 104, 111, 114, 258, 511
GsuI CTGGAG 1 cut(s) 130
HaeII RGCGCY 1 cut(s) 222
HaeIII GGCC 1 cut(s) 106
HgaI GACGC 1 cut(s) 18
HhaI GCGC 1 cut(s) 221
Hin1I GRCGYC 1 cut(s) 29
Hin1II CATG 4 cut(s) 139, 178, 334, 418
Hin6I GCGC 1 cut(s) 219
HinP1I GCGC 1 cut(s) 219
HincII GTYRAC 2 cut(s) 33, 349
HindII GTYRAC 2 cut(s) 33, 349
Hpy166II GTNNAC 3 cut(s) 33, 349, 410
Hpy188I TCNGA 2 cut(s) 17, 239
Hpy188III TCNNGA 4 cut(s) 40, 209, 331, 357
Hpy8I GTNNAC 3 cut(s) 33, 349, 410
HpyAV CCTTC 2 cut(s) 63, 266
HpyCH4III ACNGT 2 cut(s) 37, 407
HpyCH4V TGCA 7 cut(s) 116, 131, 182, 312, 475, 501, 510
HpyF10VI GCNNNNNNNGC 3 cut(s) 266, 498, 507
HpyF3I CTNAG 2 cut(s) 215, 515
Hsp92I GRCGYC 1 cut(s) 29
Hsp92II CATG 4 cut(s) 139, 178, 334, 418
HspAI GCGC 1 cut(s) 219
Kzo9I GATC 3 cut(s) 252, 292, 353
LmnI GCTCC 1 cut(s) 563
LpnPI CCDG 8 cut(s) 53, 76, 103, 117, 120, 142, 160, 470
Lsp1109I GCAGC 2 cut(s) 100, 522
LweI GCATC 4 cut(s) 169, 299, 409, 488
MaeIII GTNAC 1 cut(s) 296
MalI GATC 3 cut(s) 254, 294, 355
MboI GATC 3 cut(s) 252, 292, 353
MboII GAAGA 5 cut(s) 78, 410, 413, 466, 590
MhlI GDGCHC 1 cut(s) 216
MluCI AATT 4 cut(s) 18, 152, 326, 581
MnlI CCTC 9 cut(s) 36, 130, 163, 216, 245, 256, 276, 388, 471
Mph1103I ATGCAT 1 cut(s) 314
MseI TTAA 1 cut(s) 537
MspR9I CCNGG 1 cut(s) 91
Mva1269I GAATGC 1 cut(s) 512
MvaI CCWGG 1 cut(s) 91
MwoI GCNNNNNNNGC 3 cut(s) 266, 498, 507
NdeII GATC 3 cut(s) 252, 292, 353
NlaIII CATG 4 cut(s) 139, 178, 334, 418
NsiI ATGCAT 1 cut(s) 314
PaeR7I CTCGAG 1 cut(s) 209
PagI TCATGA 1 cut(s) 330
PctI GAATGC 1 cut(s) 512
PkrI GCNGC 5 cut(s) 105, 112, 115, 259, 512
Ple19I CGATCG 1 cut(s) 255
Psp124BI GAGCTC 1 cut(s) 216
Psp6I CCWGG 1 cut(s) 89
PspGI CCWGG 1 cut(s) 89
PvuI CGATCG 1 cut(s) 255
SacI GAGCTC 1 cut(s) 216
SaqAI TTAA 1 cut(s) 537
SatI GCNGC 5 cut(s) 104, 111, 114, 258, 511
Sau3AI GATC 3 cut(s) 252, 292, 353
ScrFI CCNGG 1 cut(s) 91
SduI GDGCHC 1 cut(s) 216
SetI ASST 7 cut(s) 95, 122, 174, 216, 271, 568, 590
SfaNI GCATC 4 cut(s) 169, 299, 409, 488
Sfr274I CTCGAG 1 cut(s) 209
SlaI CTCGAG 1 cut(s) 209
SmlI CTYRAG 1 cut(s) 209
SmoI CTYRAG 1 cut(s) 209
Sse9I AATT 4 cut(s) 18, 152, 326, 581
SsiI CCGC 3 cut(s) 103, 110, 258
SstI GAGCTC 1 cut(s) 216
StyD4I CCNGG 1 cut(s) 89
StyI CCWWGG 2 cut(s) 465, 493
TaaI ACNGT 2 cut(s) 37, 407
TaqI TCGA 3 cut(s) 210, 251, 435
TasI AATT 4 cut(s) 18, 152, 326, 581
TauI GCSGC 3 cut(s) 106, 113, 260
Tru1I TTAA 1 cut(s) 537
Tru9I TTAA 1 cut(s) 537
TscAI CASTG 1 cut(s) 345
TseI GCWGC 2 cut(s) 113, 510
TspDTI ATGAA 3 cut(s) 319, 339, 347
TspGWI ACGGA 2 cut(s) 315, 455
TspRI CASTG 1 cut(s) 345
XapI RAATTY 3 cut(s) 18, 326, 581
XhoI CTCGAG 1 cut(s) 209
Zsp2I ATGCAT 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.