RchiOBHm_Chr1g0350741

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0016387
Physical Location & Seq
Forward (+)
43903110 .. 43903688
579 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57657

Sequence Viewer

Length: 579 bp
ATGAGTTATTTTAGAAGTTGCTTGACCGCTCTCCGGCGGAGCACGTCTAGTGGATGCAAGAGCTTCCGAAAGAAGAGAAAAATGTCTTCCAACTCTGATGCACCGTCGACTACTGCATCGTCGTTTGCGGCCATGGAAGTGTCAAATCAGTTCAAGCTAGTGTTTGAGGTGATAGACGCAAACGGGGACGGGAAGATATCTCCGGTGGAGCTCAGTGAAGTTCTCTGCTGCCTCGGGTACAAGAACAAGTCCGCGGCGGTGAAAGAAGCCGAAGGAATGGTGAGAGAGATGGACTGCAATGGAGACGGGTTCATTGACTTGGATGAGTTCATGAATGCTGTGAATGTTAATGATACGAAGAGTAATGGCTGTGATGATCTCATGGATGCTTTTCTCATATTTGATACTGATAAGAACGGAAAGATTTCAGCCAAGGAGTTGAGGAGGGTGCTAGTTAGCCTTGGATGTGAAAAAAGCAGTCTCAAAGAGTGTAAGCAAATGATCAAAGGGGTTGATAAGAATGGAGATGGGGTGGTGGATTTTGAAGAATTTAGGTTGATGATGACAAAAAATGTTTAA

Protein Analysis

192

Amino Acids

21.16

Weight (kDa)

5.7

Isoelectric Point (pI)

38.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 51 - 113 1.1e-10 EF-hand domain pair
EF-hand_5 PF13202 52 - 74 9.9e-06 EF hand
EF-hand_1 PF00036 91 - 114 7.2e-06 EF hand domain
EF-hand_1 PF00036 126 - 153 1.6e-06 EF hand domain
EF-hand_6 PF13405 126 - 155 5e-08 EF-hand domain
EF-hand_7 PF13499 126 - 188 7.2e-13 EF-hand domain pair
EF-hand_5 PF13202 128 - 151 1e-06 EF hand
EF-hand_8 PF13833 139 - 190 1.7e-07 EF-hand domain pair
EF-hand_1 PF00036 163 - 190 3.2e-08 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016387)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g12030
malus_domestica MD02G1202000.v1.1 MD07G1123300.v1.1
prunus_persica Prupe.2G146900_v2.0.a1
pyrus_communis pycom07g11200
rosa_chinensis RchiOBHm_Chr1g0350741
rosa_laevigata RLG00000028458
rosa_multiflora Rmu_sc0007810.1_g000002
rosa_roxburghii Rroxscaffold_4G00304540
rosa_rugosa Rorug01G0213700
rosa_samantha Rh1AG227900 Rh1BG197400 Rh1CG212800 Rh1DG225300
rosa_wichuraiana Rw1G019740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 29
AccI GTMKAC 1 cut(s) 107
AccII CGCG 1 cut(s) 254
AciI CCGC 6 cut(s) 27, 37, 128, 252, 254, 257
AcoI YGGCCR 1 cut(s) 129
AcsI RAATTY 1 cut(s) 548
AfaI GTAC 1 cut(s) 239
AfiI CCNNNNNNNGG 1 cut(s) 33
AgsI TTSAA 2 cut(s) 154, 545
AjiI CACGTC 1 cut(s) 45
AluBI AGCT 3 cut(s) 63, 157, 211
AluI AGCT 3 cut(s) 63, 157, 211
Alw21I GWGCWC 2 cut(s) 44, 213
Alw26I GTCTC 2 cut(s) 297, 485
Ama87I CYCGRG 1 cut(s) 233
AoxI GGCC 1 cut(s) 129
ApeKI GCWGC 1 cut(s) 228
ApoI RAATTY 1 cut(s) 548
Asp700I GAANNNNTTC 1 cut(s) 424
AsuHPI GGTGA 3 cut(s) 181, 271, 292
AvaI CYCGRG 1 cut(s) 233
BanII GRGCYC 1 cut(s) 213
BbsI GAAGAC 1 cut(s) 78
Bbv12I GWGCWC 2 cut(s) 44, 213
BbvI GCAGC 1 cut(s) 215
BccI CCATC 2 cut(s) 283, 521
BclI TGATCA 1 cut(s) 501
BcoDI GTCTC 2 cut(s) 297, 485
BfaI CTAG 3 cut(s) 48, 158, 452
BisI GCNGC 3 cut(s) 129, 229, 255
BlsI GCNGC 3 cut(s) 130, 230, 256
BmeT110I CYCGRG 1 cut(s) 233
BmgBI CACGTC 1 cut(s) 45
BmsI GCATC 4 cut(s) 44, 88, 125, 376
BpiI GAAGAC 1 cut(s) 78
BsaJI CCNNGG 5 cut(s) 132, 232, 252, 432, 460
BsaWI WCCGGW 1 cut(s) 202
BsaXI ACNNNNNCTCC 2 cut(s) 516, 546
Bsc4I CCNNNNNNNGG 1 cut(s) 33
Bse3DI GCAATG 1 cut(s) 304
BseDI CCNNGG 5 cut(s) 132, 232, 252, 432, 460
BseGI GGATG 4 cut(s) 59, 328, 391, 470
BseLI CCNNNNNNNGG 1 cut(s) 33
BseMI GCAATG 1 cut(s) 304
BseMII CTCAG 1 cut(s) 226
BseRI GAGGAG 1 cut(s) 457
BseXI GCAGC 1 cut(s) 215
Bsh1236I CGCG 1 cut(s) 254
BshFI GGCC 1 cut(s) 131
BsiHKAI GWGCWC 2 cut(s) 44, 213
BsiHKCI CYCGRG 1 cut(s) 233
BsiSI CCGG 2 cut(s) 34, 203
BslFI GGGAC 1 cut(s) 200
BslI CCNNNNNNNGG 1 cut(s) 33
BsmAI GTCTC 2 cut(s) 297, 485
BsmBI CGTCTC 1 cut(s) 297
BsmFI GGGAC 1 cut(s) 200
BsmI GAATGC 1 cut(s) 340
BsnI GGCC 1 cut(s) 131
BsoBI CYCGRG 1 cut(s) 233
Bsp1286I GDGCHC 2 cut(s) 44, 213
Bsp143I GATC 2 cut(s) 376, 501
Bsp19I CCATGG 1 cut(s) 132
BspACI CCGC 6 cut(s) 27, 37, 128, 252, 254, 257
BspANI GGCC 1 cut(s) 131
BspCNI CTCAG 1 cut(s) 225
BspFNI CGCG 1 cut(s) 254
BspHI TCATGA 1 cut(s) 330
BsrBI CCGCTC 1 cut(s) 29
BsrDI GCAATG 1 cut(s) 304
BssECI CCNNGG 5 cut(s) 132, 232, 252, 432, 460
BssMI GATC 2 cut(s) 376, 501
BssT1I CCWWGG 3 cut(s) 132, 432, 460
Bst4CI ACNGT 1 cut(s) 105
Bst6I CTCTTC 2 cut(s) 68, 353
BstDEI CTNAG 1 cut(s) 212
BstDSI CCRYGG 2 cut(s) 132, 252
BstF5I GGATG 4 cut(s) 59, 328, 391, 470
BstFNI CGCG 1 cut(s) 254
BstKTI GATC 2 cut(s) 379, 504
BstMAI GTCTC 2 cut(s) 297, 485
BstMBI GATC 2 cut(s) 376, 501
BstUI CGCG 1 cut(s) 254
BstV1I GCAGC 1 cut(s) 215
BstV2I GAAGAC 1 cut(s) 78
BsuRI GGCC 1 cut(s) 131
BtgI CCRYGG 2 cut(s) 132, 252
BtrI CACGTC 1 cut(s) 45
BtsCI GGATG 4 cut(s) 59, 328, 391, 470
BtsIMutI CAGTG 1 cut(s) 220
CciI TCATGA 1 cut(s) 330
Cfr42I CCGCGG 1 cut(s) 255
CseI GACGC 1 cut(s) 185
Csp6I GTAC 1 cut(s) 238
CviAII CATG 3 cut(s) 133, 331, 382
CviJI RGCY 8 cut(s) 63, 131, 157, 211, 269, 369, 431, 459
CviKI_1 RGCY 8 cut(s) 63, 131, 157, 211, 269, 369, 431, 459
CviQI GTAC 1 cut(s) 238
DdeI CTNAG 1 cut(s) 212
DpnI GATC 2 cut(s) 378, 503
DpnII GATC 2 cut(s) 376, 501
EaeI YGGCCR 1 cut(s) 129
Eam1104I CTCTTC 2 cut(s) 68, 353
EarI CTCTTC 2 cut(s) 68, 353
EciI GGCGGA 1 cut(s) 52
Ecl136II GAGCTC 1 cut(s) 211
Eco130I CCWWGG 3 cut(s) 132, 432, 460
Eco24I GRGCYC 1 cut(s) 213
Eco32I GATATC 1 cut(s) 198
Eco53kI GAGCTC 1 cut(s) 211
Eco88I CYCGRG 1 cut(s) 233
EcoICRI GAGCTC 1 cut(s) 211
EcoRV GATATC 1 cut(s) 198
EcoT14I CCWWGG 3 cut(s) 132, 432, 460
EcoT38I GRGCYC 1 cut(s) 213
ErhI CCWWGG 3 cut(s) 132, 432, 460
Esp3I CGTCTC 1 cut(s) 297
FaeI CATG 3 cut(s) 136, 334, 385
FaiI YATR 4 cut(s) 134, 332, 383, 398
FaqI GGGAC 1 cut(s) 200
FatI CATG 3 cut(s) 132, 330, 381
FbaI TGATCA 1 cut(s) 501
FblI GTMKAC 1 cut(s) 107
Fnu4HI GCNGC 3 cut(s) 129, 229, 255
FokI GGATG 4 cut(s) 66, 335, 398, 477
FriOI GRGCYC 1 cut(s) 213
Fsp4HI GCNGC 3 cut(s) 129, 229, 255
FspBI CTAG 3 cut(s) 48, 158, 452
GluI GCNGC 3 cut(s) 129, 229, 255
HaeIII GGCC 1 cut(s) 131
HapII CCGG 2 cut(s) 34, 203
HgaI GACGC 1 cut(s) 185
Hin1II CATG 3 cut(s) 136, 334, 385
HincII GTYRAC 1 cut(s) 108
HindII GTYRAC 1 cut(s) 108
HpaII CCGG 2 cut(s) 34, 203
HphI GGTGA 3 cut(s) 181, 271, 292
Hpy166II GTNNAC 1 cut(s) 108
Hpy188I TCNGA 2 cut(s) 68, 97
Hpy188III TCNNGA 1 cut(s) 331
Hpy8I GTNNAC 1 cut(s) 108
Hpy99I CGWCG 2 cut(s) 109, 124
HpyAV CCTTC 1 cut(s) 266
HpyCH4III ACNGT 1 cut(s) 105
HpyCH4IV ACGT 1 cut(s) 44
HpyCH4V TGCA 4 cut(s) 57, 101, 116, 297
HpyF3I CTNAG 1 cut(s) 212
HpySE526I ACGT 1 cut(s) 44
Hsp92II CATG 3 cut(s) 136, 334, 385
Ksp22I TGATCA 1 cut(s) 501
KspI CCGCGG 1 cut(s) 255
Kzo9I GATC 2 cut(s) 376, 501
LmnI GCTCC 2 cut(s) 39, 208
LpnPI CCDG 2 cut(s) 47, 216
Lsp1109I GCAGC 1 cut(s) 215
LweI GCATC 4 cut(s) 44, 88, 125, 376
MaeI CTAG 3 cut(s) 48, 158, 452
MaeII ACGT 1 cut(s) 44
MalI GATC 2 cut(s) 378, 503
MbiI CCGCTC 1 cut(s) 29
MboI GATC 2 cut(s) 376, 501
MboII GAAGA 5 cut(s) 78, 85, 205, 370, 557
MhlI GDGCHC 2 cut(s) 44, 213
MluCI AATT 1 cut(s) 548
MmeI TCCRAC 1 cut(s) 114
MnlI CCTC 4 cut(s) 160, 242, 435, 438
MroXI GAANNNNTTC 1 cut(s) 424
MseI TTAA 2 cut(s) 348, 577
MslI CAYNNNNRTG 1 cut(s) 137
MspA1I CMGCKG 1 cut(s) 254
MspI CCGG 2 cut(s) 34, 203
Mva1269I GAATGC 1 cut(s) 340
MvnI CGCG 1 cut(s) 254
NcoI CCATGG 1 cut(s) 132
NdeII GATC 2 cut(s) 376, 501
NlaIII CATG 3 cut(s) 136, 334, 385
PagI TCATGA 1 cut(s) 330
PctI GAATGC 1 cut(s) 340
PdmI GAANNNNTTC 1 cut(s) 424
PkrI GCNGC 3 cut(s) 130, 230, 256
Psp124BI GAGCTC 1 cut(s) 213
RsaI GTAC 1 cut(s) 239
RsaNI GTAC 1 cut(s) 238
RseI CAYNNNNRTG 1 cut(s) 137
SacI GAGCTC 1 cut(s) 213
SacII CCGCGG 1 cut(s) 255
SalI GTCGAC 1 cut(s) 106
SaqAI TTAA 2 cut(s) 348, 577
SatI GCNGC 3 cut(s) 129, 229, 255
Sau3AI GATC 2 cut(s) 376, 501
SduI GDGCHC 2 cut(s) 44, 213
SetI ASST 6 cut(s) 47, 65, 159, 171, 213, 557
SfaNI GCATC 4 cut(s) 44, 88, 125, 376
Sfr303I CCGCGG 1 cut(s) 255
SgrBI CCGCGG 1 cut(s) 255
SmiMI CAYNNNNRTG 1 cut(s) 137
Sse9I AATT 1 cut(s) 548
SsiI CCGC 6 cut(s) 27, 37, 128, 252, 254, 257
SspMI CTAG 3 cut(s) 48, 158, 452
SstI GAGCTC 1 cut(s) 213
StyI CCWWGG 3 cut(s) 132, 432, 460
TaaI ACNGT 1 cut(s) 105
TaiI ACGT 1 cut(s) 47
TaqI TCGA 1 cut(s) 107
TasI AATT 1 cut(s) 548
TauI GCSGC 2 cut(s) 131, 257
Tru1I TTAA 2 cut(s) 348, 577
Tru9I TTAA 2 cut(s) 348, 577
TscAI CASTG 1 cut(s) 220
TseI GCWGC 1 cut(s) 228
TspDTI ATGAA 3 cut(s) 301, 319, 347
TspGWI ACGGA 1 cut(s) 432
TspRI CASTG 1 cut(s) 220
XapI RAATTY 1 cut(s) 548
XmiI GTMKAC 1 cut(s) 107
XmnI GAANNNNTTC 1 cut(s) 424
XspI CTAG 3 cut(s) 48, 158, 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.