Rh1CG212800

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0016387
Physical Location & Seq
Forward (+)
45900818 .. 45902206
1389 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG212800.1

Sequence Viewer

Length: 579 bp
ATGAGTTATTTTAGAAGTTGCTTGACCGCTCTCCGGCGGAGCACGTCTAGTGGATGCAAGAGCTTCCGAAAGAAGAGAAAAATGTCTTCCAACTCTGATGCACCGGCGACTACTGCATCGTCGTTTGCGGCCATGGAAGTGTCAAATCAGTTCAAGCAAGTGTTTGAGGTGATAGACGCAAACGGGGACGGGAAGATATCTCCGGTGGAGCTCAGTGAAGTTCTCTGCTGCCTCGGGTACAAGAACAAGTCCGCGGCGGCGAAAGAAGCCGAAGGAATGGTGAGAGAGATGGACTGCAATGGAGACGGGTTCATTGACTTGGATGAGTTCATGAATGCTGTGAATGTTAATGATACGAAGAGTAGTGGCTGTGATGATCTCATGGATGCTTTTCTCATATTTGATACTGATAAGAACGGAAAGATTTCAGCCAAGGAGTTGAGGAGGGTGCTAGTTAGCCTTGGATGTGAAAAAAGCAGTCTCAAAGAGTGTAAGCAAATGATCAAAGGGGTTGATAAGAATGGAGATGGGGTGGTGGATTTTGAAGAATTTAGGTTGATGATGACAAGAAATGTTTAA

Protein Analysis

192

Amino Acids

21.13

Weight (kDa)

5.7

Isoelectric Point (pI)

42.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 50 - 113 3.1e-11 EF-hand domain pair
EF-hand_5 PF13202 51 - 74 3.1e-06 EF hand
EF-hand_1 PF00036 91 - 114 7.2e-06 EF hand domain
EF-hand_1 PF00036 126 - 153 1.6e-06 EF hand domain
EF-hand_6 PF13405 126 - 155 5e-08 EF-hand domain
EF-hand_7 PF13499 126 - 188 7.2e-13 EF-hand domain pair
EF-hand_5 PF13202 128 - 151 1e-06 EF hand
EF-hand_8 PF13833 139 - 191 1e-07 EF-hand domain pair
EF-hand_1 PF00036 163 - 190 4.2e-08 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016387)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g12030
malus_domestica MD02G1202000.v1.1 MD07G1123300.v1.1
prunus_persica Prupe.2G146900_v2.0.a1
pyrus_communis pycom07g11200
rosa_chinensis RchiOBHm_Chr1g0350741
rosa_laevigata RLG00000028458
rosa_multiflora Rmu_sc0007810.1_g000002
rosa_roxburghii Rroxscaffold_4G00304540
rosa_rugosa Rorug01G0213700
rosa_samantha Rh1AG227900 Rh1BG197400 Rh1CG212800 Rh1DG225300
rosa_wichuraiana Rw1G019740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 29
AccII CGCG 1 cut(s) 254
AciI CCGC 6 cut(s) 27, 37, 128, 252, 254, 257
AcoI YGGCCR 1 cut(s) 129
AcsI RAATTY 1 cut(s) 548
AfaI GTAC 1 cut(s) 239
AfiI CCNNNNNNNGG 1 cut(s) 33
AgsI TTSAA 2 cut(s) 154, 545
AjiI CACGTC 1 cut(s) 45
AluBI AGCT 2 cut(s) 63, 211
AluI AGCT 2 cut(s) 63, 211
Alw21I GWGCWC 2 cut(s) 44, 213
Alw26I GTCTC 2 cut(s) 297, 485
Ama87I CYCGRG 1 cut(s) 233
AoxI GGCC 1 cut(s) 129
ApeKI GCWGC 1 cut(s) 228
ApoI RAATTY 1 cut(s) 548
Asp700I GAANNNNTTC 1 cut(s) 424
AsuHPI GGTGA 2 cut(s) 181, 292
AvaI CYCGRG 1 cut(s) 233
BanII GRGCYC 1 cut(s) 213
BbsI GAAGAC 1 cut(s) 78
Bbv12I GWGCWC 2 cut(s) 44, 213
BbvI GCAGC 1 cut(s) 215
BccI CCATC 2 cut(s) 283, 521
BclI TGATCA 1 cut(s) 501
BcoDI GTCTC 2 cut(s) 297, 485
BfaI CTAG 2 cut(s) 48, 452
BisI GCNGC 4 cut(s) 129, 229, 255, 258
BlsI GCNGC 4 cut(s) 130, 230, 256, 259
BmeT110I CYCGRG 1 cut(s) 233
BmgBI CACGTC 1 cut(s) 45
BmsI GCATC 4 cut(s) 44, 88, 125, 376
BpiI GAAGAC 1 cut(s) 78
BsaJI CCNNGG 5 cut(s) 132, 232, 252, 432, 460
BsaWI WCCGGW 1 cut(s) 202
BsaXI ACNNNNNCTCC 2 cut(s) 516, 546
Bsc4I CCNNNNNNNGG 1 cut(s) 33
Bse118I RCCGGY 1 cut(s) 103
Bse3DI GCAATG 1 cut(s) 304
BseDI CCNNGG 5 cut(s) 132, 232, 252, 432, 460
BseGI GGATG 4 cut(s) 59, 328, 391, 470
BseLI CCNNNNNNNGG 1 cut(s) 33
BseMI GCAATG 1 cut(s) 304
BseMII CTCAG 1 cut(s) 226
BseRI GAGGAG 1 cut(s) 457
BseXI GCAGC 1 cut(s) 215
Bsh1236I CGCG 1 cut(s) 254
BshFI GGCC 1 cut(s) 131
BsiHKAI GWGCWC 2 cut(s) 44, 213
BsiHKCI CYCGRG 1 cut(s) 233
BsiSI CCGG 3 cut(s) 34, 104, 203
BslFI GGGAC 1 cut(s) 200
BslI CCNNNNNNNGG 1 cut(s) 33
BsmAI GTCTC 2 cut(s) 297, 485
BsmBI CGTCTC 1 cut(s) 297
BsmFI GGGAC 1 cut(s) 200
BsmI GAATGC 1 cut(s) 340
BsnI GGCC 1 cut(s) 131
BsoBI CYCGRG 1 cut(s) 233
Bsp1286I GDGCHC 2 cut(s) 44, 213
Bsp143I GATC 2 cut(s) 376, 501
Bsp19I CCATGG 1 cut(s) 132
BspACI CCGC 6 cut(s) 27, 37, 128, 252, 254, 257
BspANI GGCC 1 cut(s) 131
BspCNI CTCAG 1 cut(s) 225
BspFNI CGCG 1 cut(s) 254
BspHI TCATGA 1 cut(s) 330
BsrBI CCGCTC 1 cut(s) 29
BsrDI GCAATG 1 cut(s) 304
BsrFI RCCGGY 1 cut(s) 103
BssAI RCCGGY 1 cut(s) 103
BssECI CCNNGG 5 cut(s) 132, 232, 252, 432, 460
BssMI GATC 2 cut(s) 376, 501
BssT1I CCWWGG 3 cut(s) 132, 432, 460
Bst6I CTCTTC 2 cut(s) 68, 353
BstDEI CTNAG 1 cut(s) 212
BstDSI CCRYGG 2 cut(s) 132, 252
BstF5I GGATG 4 cut(s) 59, 328, 391, 470
BstFNI CGCG 1 cut(s) 254
BstKTI GATC 2 cut(s) 379, 504
BstMAI GTCTC 2 cut(s) 297, 485
BstMBI GATC 2 cut(s) 376, 501
BstMWI GCNNNNNNNGC 2 cut(s) 113, 266
BstUI CGCG 1 cut(s) 254
BstV1I GCAGC 1 cut(s) 215
BstV2I GAAGAC 1 cut(s) 78
BsuRI GGCC 1 cut(s) 131
BtgI CCRYGG 2 cut(s) 132, 252
BtrI CACGTC 1 cut(s) 45
BtsCI GGATG 4 cut(s) 59, 328, 391, 470
BtsIMutI CAGTG 1 cut(s) 220
CciI TCATGA 1 cut(s) 330
Cfr10I RCCGGY 1 cut(s) 103
Cfr42I CCGCGG 1 cut(s) 255
CseI GACGC 1 cut(s) 185
Csp6I GTAC 1 cut(s) 238
CviAII CATG 3 cut(s) 133, 331, 382
CviJI RGCY 7 cut(s) 63, 131, 211, 269, 369, 431, 459
CviKI_1 RGCY 7 cut(s) 63, 131, 211, 269, 369, 431, 459
CviQI GTAC 1 cut(s) 238
DdeI CTNAG 1 cut(s) 212
DpnI GATC 2 cut(s) 378, 503
DpnII GATC 2 cut(s) 376, 501
EaeI YGGCCR 1 cut(s) 129
Eam1104I CTCTTC 2 cut(s) 68, 353
EarI CTCTTC 2 cut(s) 68, 353
EciI GGCGGA 1 cut(s) 52
Ecl136II GAGCTC 1 cut(s) 211
Eco130I CCWWGG 3 cut(s) 132, 432, 460
Eco24I GRGCYC 1 cut(s) 213
Eco32I GATATC 1 cut(s) 198
Eco53kI GAGCTC 1 cut(s) 211
Eco88I CYCGRG 1 cut(s) 233
EcoICRI GAGCTC 1 cut(s) 211
EcoRV GATATC 1 cut(s) 198
EcoT14I CCWWGG 3 cut(s) 132, 432, 460
EcoT38I GRGCYC 1 cut(s) 213
ErhI CCWWGG 3 cut(s) 132, 432, 460
Esp3I CGTCTC 1 cut(s) 297
FaeI CATG 3 cut(s) 136, 334, 385
FaiI YATR 4 cut(s) 134, 332, 383, 398
FaqI GGGAC 1 cut(s) 200
FatI CATG 3 cut(s) 132, 330, 381
FbaI TGATCA 1 cut(s) 501
Fnu4HI GCNGC 4 cut(s) 129, 229, 255, 258
FokI GGATG 4 cut(s) 66, 335, 398, 477
FriOI GRGCYC 1 cut(s) 213
Fsp4HI GCNGC 4 cut(s) 129, 229, 255, 258
FspBI CTAG 2 cut(s) 48, 452
GluI GCNGC 4 cut(s) 129, 229, 255, 258
HaeIII GGCC 1 cut(s) 131
HapII CCGG 3 cut(s) 34, 104, 203
HgaI GACGC 1 cut(s) 185
Hin1II CATG 3 cut(s) 136, 334, 385
HpaII CCGG 3 cut(s) 34, 104, 203
HphI GGTGA 2 cut(s) 181, 292
Hpy188I TCNGA 2 cut(s) 68, 97
Hpy188III TCNNGA 1 cut(s) 331
Hpy99I CGWCG 1 cut(s) 124
HpyAV CCTTC 1 cut(s) 266
HpyCH4IV ACGT 1 cut(s) 44
HpyCH4V TGCA 4 cut(s) 57, 101, 116, 297
HpyF10VI GCNNNNNNNGC 2 cut(s) 113, 266
HpyF3I CTNAG 1 cut(s) 212
HpySE526I ACGT 1 cut(s) 44
Hsp92II CATG 3 cut(s) 136, 334, 385
Ksp22I TGATCA 1 cut(s) 501
KspI CCGCGG 1 cut(s) 255
Kzo9I GATC 2 cut(s) 376, 501
LmnI GCTCC 2 cut(s) 39, 208
LpnPI CCDG 3 cut(s) 47, 117, 216
Lsp1109I GCAGC 1 cut(s) 215
LweI GCATC 4 cut(s) 44, 88, 125, 376
MaeI CTAG 2 cut(s) 48, 452
MaeII ACGT 1 cut(s) 44
MalI GATC 2 cut(s) 378, 503
MbiI CCGCTC 1 cut(s) 29
MboI GATC 2 cut(s) 376, 501
MboII GAAGA 5 cut(s) 78, 85, 205, 370, 557
MhlI GDGCHC 2 cut(s) 44, 213
MluCI AATT 1 cut(s) 548
MmeI TCCRAC 1 cut(s) 114
MnlI CCTC 4 cut(s) 160, 242, 435, 438
MroXI GAANNNNTTC 1 cut(s) 424
MseI TTAA 2 cut(s) 348, 577
MslI CAYNNNNRTG 1 cut(s) 137
MspA1I CMGCKG 1 cut(s) 254
MspI CCGG 3 cut(s) 34, 104, 203
Mva1269I GAATGC 1 cut(s) 340
MvnI CGCG 1 cut(s) 254
MwoI GCNNNNNNNGC 2 cut(s) 113, 266
NcoI CCATGG 1 cut(s) 132
NdeII GATC 2 cut(s) 376, 501
NlaIII CATG 3 cut(s) 136, 334, 385
PagI TCATGA 1 cut(s) 330
PctI GAATGC 1 cut(s) 340
PdmI GAANNNNTTC 1 cut(s) 424
PkrI GCNGC 4 cut(s) 130, 230, 256, 259
Psp124BI GAGCTC 1 cut(s) 213
RsaI GTAC 1 cut(s) 239
RsaNI GTAC 1 cut(s) 238
RseI CAYNNNNRTG 1 cut(s) 137
SacI GAGCTC 1 cut(s) 213
SacII CCGCGG 1 cut(s) 255
SaqAI TTAA 2 cut(s) 348, 577
SatI GCNGC 4 cut(s) 129, 229, 255, 258
Sau3AI GATC 2 cut(s) 376, 501
SduI GDGCHC 2 cut(s) 44, 213
SetI ASST 5 cut(s) 47, 65, 171, 213, 557
SfaNI GCATC 4 cut(s) 44, 88, 125, 376
Sfr303I CCGCGG 1 cut(s) 255
SgrAI CRCCGGYG 1 cut(s) 103
SgrBI CCGCGG 1 cut(s) 255
SmiMI CAYNNNNRTG 1 cut(s) 137
Sse9I AATT 1 cut(s) 548
SsiI CCGC 6 cut(s) 27, 37, 128, 252, 254, 257
SspMI CTAG 2 cut(s) 48, 452
SstI GAGCTC 1 cut(s) 213
StyI CCWWGG 3 cut(s) 132, 432, 460
TaiI ACGT 1 cut(s) 47
TasI AATT 1 cut(s) 548
TauI GCSGC 3 cut(s) 131, 257, 260
Tru1I TTAA 2 cut(s) 348, 577
Tru9I TTAA 2 cut(s) 348, 577
TscAI CASTG 1 cut(s) 220
TseI GCWGC 1 cut(s) 228
TspDTI ATGAA 3 cut(s) 301, 319, 347
TspGWI ACGGA 1 cut(s) 432
TspRI CASTG 1 cut(s) 220
XapI RAATTY 1 cut(s) 548
XmnI GAANNNNTTC 1 cut(s) 424
XspI CTAG 2 cut(s) 48, 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.