RLG00000028458

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0016387
Physical Location & Seq
Reverse (-)
23049073 .. 23050096
1024 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028458

Sequence Viewer

Length: 681 bp
ATGTCTTCCAACTCTGATGCACCGGCGACTATTGCATCGTCGTTTGCGGCCATGGAAGTGTCAAATCAGTTCAAGCAAGTGTTTGAGGTGATGGACGCAAACGGGGACGGGAAGATATCTCCGGTGGAGCTCAGTGAAGTTCTCTGCTGCCTCGGGTACAAGAACAAGTCCGCGGCGGCGAAAGAAGCCGAAGGGATGGTGAGAGAGATGGACTGCAATGGAGACGGGTTCATTGACTTAGATGAGTTCATGAATGCTGTGAATGTTAATGATACGAACAGTAATGGCTGTGATGATCTCATGGATGCTTTTCTTATATTTGATACTGATAAGAACGGAAAGATTTCAGCCAAGGAGTTGAGGAGGGTGCTAGTTAGCCTTGGATGTGAAAAAAGCAGTCTCAAAGAGTGTAAGCAAATGATCAAAGGGGTTGATAAGAATGGAGATGGGGTTGTGGATTTTGAAGAATTTAGAAAAAGAAACAAGAATGCGATACTGCGATACATATGGCAGTCTTATTCTCATGATACGGAGCACGTGGAAGGCTGTGGGAGTCCATTGGCATGGCACTTCAAAGTAGAAGCATACGTGGCTTCAATTAATTACCATGTCATCCACAGGTTGTTTGGATCTGTTCGAGGGGGTGGACTCGGTGTAATCGGTTTGAGGGTTGAATCTTAG

Protein Analysis

227

Amino Acids

24.83

Weight (kDa)

5.09

Isoelectric Point (pI)

30.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 23 - 86 7.3e-11 EF-hand domain pair
EF-hand_5 PF13202 24 - 47 4.9e-06 EF hand
EF-hand_1 PF00036 64 - 87 9.1e-06 EF hand domain
EF-hand_1 PF00036 99 - 126 2.1e-06 EF hand domain
EF-hand_7 PF13499 99 - 159 2.5e-12 EF-hand domain pair
EF-hand_6 PF13405 99 - 128 6.4e-08 EF-hand domain
EF-hand_5 PF13202 101 - 124 1.3e-06 EF hand
EF-hand_8 PF13833 112 - 158 9.8e-07 EF-hand domain pair
EF-hand_1 PF00036 136 - 159 2.3e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016387)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g12030
malus_domestica MD02G1202000.v1.1 MD07G1123300.v1.1
prunus_persica Prupe.2G146900_v2.0.a1
pyrus_communis pycom07g11200
rosa_chinensis RchiOBHm_Chr1g0350741
rosa_laevigata RLG00000028458
rosa_multiflora Rmu_sc0007810.1_g000002
rosa_roxburghii Rroxscaffold_4G00304540
rosa_rugosa Rorug01G0213700
rosa_samantha Rh1AG227900 Rh1BG197400 Rh1CG212800 Rh1DG225300
rosa_wichuraiana Rw1G019740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 173
AciI CCGC 4 cut(s) 47, 171, 173, 176
AclWI GGATC 1 cut(s) 637
AcoI YGGCCR 1 cut(s) 48
AcsI RAATTY 1 cut(s) 467
AcvI CACGTG 1 cut(s) 538
AfaI GTAC 1 cut(s) 158
AgsI TTSAA 5 cut(s) 73, 464, 574, 597, 674
AluBI AGCT 1 cut(s) 130
AluI AGCT 1 cut(s) 130
Alw21I GWGCWC 2 cut(s) 132, 537
Alw26I GTCTC 2 cut(s) 216, 404
AlwI GGATC 1 cut(s) 637
Ama87I CYCGRG 1 cut(s) 152
AoxI GGCC 1 cut(s) 48
ApeKI GCWGC 1 cut(s) 147
ApoI RAATTY 1 cut(s) 467
AseI ATTAAT 1 cut(s) 600
Asp700I GAANNNNTTC 1 cut(s) 343
AsuHPI GGTGA 2 cut(s) 100, 211
AvaI CYCGRG 1 cut(s) 152
BanII GRGCYC 1 cut(s) 132
BbrPI CACGTG 1 cut(s) 538
Bbv12I GWGCWC 2 cut(s) 132, 537
BbvI GCAGC 1 cut(s) 134
BccI CCATC 4 cut(s) 85, 190, 202, 440
BclI TGATCA 1 cut(s) 420
BcoDI GTCTC 2 cut(s) 216, 404
BfaI CTAG 1 cut(s) 371
BisI GCNGC 4 cut(s) 48, 148, 174, 177
BlsI GCNGC 4 cut(s) 49, 149, 175, 178
BmeT110I CYCGRG 1 cut(s) 152
BmsI GCATC 3 cut(s) 7, 44, 295
BsaAI YACGTR 2 cut(s) 538, 589
BsaJI CCNNGG 5 cut(s) 51, 151, 171, 351, 379
BsaWI WCCGGW 1 cut(s) 121
BsaXI ACNNNNNCTCC 2 cut(s) 435, 465
Bse118I RCCGGY 1 cut(s) 22
Bse3DI GCAATG 1 cut(s) 223
BseDI CCNNGG 5 cut(s) 51, 151, 171, 351, 379
BseGI GGATG 4 cut(s) 201, 310, 389, 612
BseMI GCAATG 1 cut(s) 223
BseMII CTCAG 1 cut(s) 145
BseRI GAGGAG 1 cut(s) 376
BseXI GCAGC 1 cut(s) 134
Bsh1236I CGCG 1 cut(s) 173
BshFI GGCC 1 cut(s) 50
BsiHKAI GWGCWC 2 cut(s) 132, 537
BsiHKCI CYCGRG 1 cut(s) 152
BsiSI CCGG 2 cut(s) 23, 122
BslFI GGGAC 1 cut(s) 119
BsmAI GTCTC 2 cut(s) 216, 404
BsmBI CGTCTC 1 cut(s) 216
BsmFI GGGAC 1 cut(s) 119
BsmI GAATGC 2 cut(s) 259, 493
BsnI GGCC 1 cut(s) 50
BsoBI CYCGRG 1 cut(s) 152
Bsp1286I GDGCHC 2 cut(s) 132, 537
Bsp143I GATC 3 cut(s) 295, 420, 629
Bsp19I CCATGG 1 cut(s) 51
BspACI CCGC 4 cut(s) 47, 171, 173, 176
BspANI GGCC 1 cut(s) 50
BspCNI CTCAG 1 cut(s) 144
BspFNI CGCG 1 cut(s) 173
BspHI TCATGA 2 cut(s) 249, 523
BspPI GGATC 1 cut(s) 637
BsrDI GCAATG 1 cut(s) 223
BsrFI RCCGGY 1 cut(s) 22
BssAI RCCGGY 1 cut(s) 22
BssECI CCNNGG 5 cut(s) 51, 151, 171, 351, 379
BssMI GATC 3 cut(s) 295, 420, 629
BssT1I CCWWGG 3 cut(s) 51, 351, 379
Bst4CI ACNGT 1 cut(s) 281
BstBAI YACGTR 2 cut(s) 538, 589
BstDEI CTNAG 3 cut(s) 131, 238, 678
BstDSI CCRYGG 2 cut(s) 51, 171
BstF5I GGATG 4 cut(s) 201, 310, 389, 612
BstFNI CGCG 1 cut(s) 173
BstKTI GATC 3 cut(s) 298, 423, 632
BstMAI GTCTC 2 cut(s) 216, 404
BstMBI GATC 3 cut(s) 295, 420, 629
BstMWI GCNNNNNNNGC 3 cut(s) 32, 185, 590
BstUI CGCG 1 cut(s) 173
BstV1I GCAGC 1 cut(s) 134
BstX2I RGATCY 1 cut(s) 629
BstXI CCANNNNNNTGG 1 cut(s) 564
BstYI RGATCY 1 cut(s) 629
BsuRI GGCC 1 cut(s) 50
BtgI CCRYGG 2 cut(s) 51, 171
BtsCI GGATG 4 cut(s) 201, 310, 389, 612
BtsIMutI CAGTG 1 cut(s) 139
CciI TCATGA 2 cut(s) 249, 523
Cfr10I RCCGGY 1 cut(s) 22
Cfr42I CCGCGG 1 cut(s) 174
CseI GACGC 1 cut(s) 104
Csp6I GTAC 1 cut(s) 157
CviAII CATG 6 cut(s) 52, 250, 301, 524, 564, 608
CviJI RGCY 8 cut(s) 50, 130, 188, 288, 350, 378, 546, 593
CviKI_1 RGCY 8 cut(s) 50, 130, 188, 288, 350, 378, 546, 593
CviQI GTAC 1 cut(s) 157
DdeI CTNAG 3 cut(s) 131, 238, 678
DpnI GATC 3 cut(s) 297, 422, 631
DpnII GATC 3 cut(s) 295, 420, 629
EaeI YGGCCR 1 cut(s) 48
Ecl136II GAGCTC 1 cut(s) 130
Eco130I CCWWGG 3 cut(s) 51, 351, 379
Eco24I GRGCYC 1 cut(s) 132
Eco32I GATATC 1 cut(s) 117
Eco53kI GAGCTC 1 cut(s) 130
Eco72I CACGTG 1 cut(s) 538
Eco88I CYCGRG 1 cut(s) 152
EcoICRI GAGCTC 1 cut(s) 130
EcoRV GATATC 1 cut(s) 117
EcoT14I CCWWGG 3 cut(s) 51, 351, 379
EcoT38I GRGCYC 1 cut(s) 132
ErhI CCWWGG 3 cut(s) 51, 351, 379
Esp3I CGTCTC 1 cut(s) 216
FaeI CATG 6 cut(s) 55, 253, 304, 527, 567, 611
FaqI GGGAC 1 cut(s) 119
FatI CATG 6 cut(s) 51, 249, 300, 523, 563, 607
FauNDI CATATG 1 cut(s) 506
FbaI TGATCA 1 cut(s) 420
Fnu4HI GCNGC 4 cut(s) 48, 148, 174, 177
FokI GGATG 4 cut(s) 208, 317, 396, 599
FriOI GRGCYC 1 cut(s) 132
Fsp4HI GCNGC 4 cut(s) 48, 148, 174, 177
FspBI CTAG 1 cut(s) 371
GluI GCNGC 4 cut(s) 48, 148, 174, 177
HaeIII GGCC 1 cut(s) 50
HapII CCGG 2 cut(s) 23, 122
HgaI GACGC 1 cut(s) 104
Hin1II CATG 6 cut(s) 55, 253, 304, 527, 567, 611
HinfI GANTC 3 cut(s) 553, 648, 674
HpaII CCGG 2 cut(s) 23, 122
HphI GGTGA 2 cut(s) 100, 211
Hpy166II GTNNAC 1 cut(s) 647
Hpy188I TCNGA 1 cut(s) 16
Hpy188III TCNNGA 2 cut(s) 250, 524
Hpy8I GTNNAC 1 cut(s) 647
Hpy99I CGWCG 1 cut(s) 43
HpyAV CCTTC 2 cut(s) 185, 536
HpyCH4III ACNGT 1 cut(s) 281
HpyCH4IV ACGT 2 cut(s) 537, 588
HpyCH4V TGCA 3 cut(s) 20, 35, 216
HpyF10VI GCNNNNNNNGC 3 cut(s) 32, 185, 590
HpyF3I CTNAG 3 cut(s) 131, 238, 678
HpySE526I ACGT 2 cut(s) 537, 588
Hsp92II CATG 6 cut(s) 55, 253, 304, 527, 567, 611
Ksp22I TGATCA 1 cut(s) 420
KspI CCGCGG 1 cut(s) 174
Kzo9I GATC 3 cut(s) 295, 420, 629
LmnI GCTCC 2 cut(s) 127, 532
LpnPI CCDG 3 cut(s) 36, 135, 604
Lsp1109I GCAGC 1 cut(s) 134
LweI GCATC 3 cut(s) 7, 44, 295
MaeI CTAG 1 cut(s) 371
MaeII ACGT 2 cut(s) 537, 588
MalI GATC 3 cut(s) 297, 422, 631
MboI GATC 3 cut(s) 295, 420, 629
MboII GAAGA 2 cut(s) 124, 476
MflI RGATCY 1 cut(s) 629
MhlI GDGCHC 2 cut(s) 132, 537
MluCI AATT 3 cut(s) 467, 597, 601
MlyI GAGTC 2 cut(s) 562, 642
MmeI TCCRAC 1 cut(s) 33
MnlI CCTC 6 cut(s) 79, 161, 354, 357, 632, 660
MroXI GAANNNNTTC 1 cut(s) 343
MseI TTAA 2 cut(s) 267, 600
MslI CAYNNNNRTG 2 cut(s) 56, 562
MspA1I CMGCKG 1 cut(s) 173
MspI CCGG 2 cut(s) 23, 122
Mva1269I GAATGC 2 cut(s) 259, 493
MvnI CGCG 1 cut(s) 173
MwoI GCNNNNNNNGC 3 cut(s) 32, 185, 590
NcoI CCATGG 1 cut(s) 51
NdeI CATATG 1 cut(s) 506
NdeII GATC 3 cut(s) 295, 420, 629
NlaIII CATG 6 cut(s) 55, 253, 304, 527, 567, 611
PagI TCATGA 2 cut(s) 249, 523
PctI GAATGC 2 cut(s) 259, 493
PdmI GAANNNNTTC 1 cut(s) 343
PfeI GAWTC 1 cut(s) 674
PkrI GCNGC 4 cut(s) 49, 149, 175, 178
PleI GAGTC 2 cut(s) 561, 642
PmaCI CACGTG 1 cut(s) 538
PmlI CACGTG 1 cut(s) 538
PpsI GAGTC 2 cut(s) 561, 642
Ppu21I YACGTR 2 cut(s) 538, 589
PshBI ATTAAT 1 cut(s) 600
Psp124BI GAGCTC 1 cut(s) 132
PspCI CACGTG 1 cut(s) 538
PsuI RGATCY 1 cut(s) 629
RsaI GTAC 1 cut(s) 158
RsaNI GTAC 1 cut(s) 157
RseI CAYNNNNRTG 2 cut(s) 56, 562
SacI GAGCTC 1 cut(s) 132
SacII CCGCGG 1 cut(s) 174
SaqAI TTAA 2 cut(s) 267, 600
SatI GCNGC 4 cut(s) 48, 148, 174, 177
Sau3AI GATC 3 cut(s) 295, 420, 629
SchI GAGTC 2 cut(s) 562, 642
SduI GDGCHC 2 cut(s) 132, 537
SetI ASST 5 cut(s) 90, 132, 540, 591, 623
SfaNI GCATC 3 cut(s) 7, 44, 295
Sfr303I CCGCGG 1 cut(s) 174
SgrAI CRCCGGYG 1 cut(s) 22
SgrBI CCGCGG 1 cut(s) 174
SmiMI CAYNNNNRTG 2 cut(s) 56, 562
Sse9I AATT 3 cut(s) 467, 597, 601
SsiI CCGC 4 cut(s) 47, 171, 173, 176
SspMI CTAG 1 cut(s) 371
SstI GAGCTC 1 cut(s) 132
StyI CCWWGG 3 cut(s) 51, 351, 379
TaaI ACNGT 1 cut(s) 281
TaiI ACGT 2 cut(s) 540, 591
TaqI TCGA 1 cut(s) 637
TasI AATT 3 cut(s) 467, 597, 601
TauI GCSGC 3 cut(s) 50, 176, 179
TfiI GAWTC 1 cut(s) 674
Tru1I TTAA 2 cut(s) 267, 600
Tru9I TTAA 2 cut(s) 267, 600
TscAI CASTG 1 cut(s) 139
TseI GCWGC 1 cut(s) 147
TspDTI ATGAA 3 cut(s) 220, 238, 266
TspGWI ACGGA 2 cut(s) 351, 545
TspRI CASTG 1 cut(s) 139
VspI ATTAAT 1 cut(s) 600
XapI RAATTY 1 cut(s) 467
XcmI CCANNNNNNNNNTGG 1 cut(s) 623
XmnI GAANNNNTTC 1 cut(s) 343
XspI CTAG 1 cut(s) 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.