FvH4_7g12110

Ankyrin repeat and zinc finger domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0007214
Physical Location & Seq
Reverse (-)
11018032 .. 11023498
5467 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g12110.t3

Sequence Viewer

Length: 1641 bp
ATGGCGTCCGAGCCTCCCCGCAGCACCACTGCCACGTCATCCACCGCAGCAAAAGAGAAACGACACCGTTCCATCTTCGAACTCCCGGCCAACTTCTTCGACTCCTGCGCGCTCCTCACTTCCTCCGGCCTCTCAGTTGCTTCAACCTTCGAGGCCTCTAACACCACCAACAGCTCCGCCCTCGAAACCCTAGACGAGTCTTTGACTGAGAACACCGATCAAGACCGCTCCCAAAGCGGCGCCGTTTCGACTCGGCTGACCTGCAACACTTGCAAGGCTGAGTTCGAGTCCCTCCAGGACCAGCGCTTTCACTTCAAATCCGATATTCACCGATTCAATGTGAAGCTAAGCCTTGCCGGGAAGAATATTGTGAAGGAGGAGGACTTTGATGAGCTGACGTCTGATTCTTTTAAAGACTTTGATGTCTCGAGTATATCGGGATCGGAGGACGATGAACCTGAAAAAGTCGGGCCTTACAAGCTTTCAAGGGCATCTGCAAGGCAGAAATTATTCATCCACCTTCAGACGGGAGAAAGAGTTTCGGTTTGGAAGTGTTTGATTACTAACGAATCTGGACCTGAAAGTGATTTGTCATGTGATGAATTGTTAGGGAGATTGAACAAGGTGGTTCATGAGCCTAGAGATAGTACCTGTTTTAGAGTTGTGTTGCTTGCAAGTGGTGGGCACTTTGCTGGTTGTGTGTTTGATGGTAACAAGATTGTTGCTCACAAGACCTTCCACAGATATGTCGTAAGGGCCAAATCTGGAAAAAAACAGTCATCAAATGATGCATCTAGGTTTGCTCATTCTGCTGGAGCTTCTCTTCGTCGCCATAATGAACTTGCATTGAAAAAGGATATTCAAGAGCTACTTGCTGCTTGGAAGCCTTACTTCGATGCTTCGTCTTGCGTTTTCGTTTATGCCCCTTCTAGCAATCATCAGCTTCTATCTAACGGGGAGAAACCATATTTTAGTCATCATAAAGTCAGGAATATTCCAATCACTATTCGGAGACCTACTATGAAGGAAGCCCGGCGGATATATGAACAGTTGACACAAGTTTCTAATGAACAAGAGGAGATTGAAGCTCCTTTAGCTACCAAAAAGGAACTACCCTCTAGTTCAGGGATTGAAAATGATATCATCTCAAGCATGGCGAAAGTCAACATTATTGAGCCTAGTACAGAAGCTTGCTCAAGTACTAGTGAGTCCAATGAACTACCCATATCAAGTGAGAGTGATAATGTGGTTAAAGGTTCGTCAACACCTTTGCATGAAGCAGTACAGTCTGGGAATGCTCTCAAGGTTTTGGAACTCTTGGAACAGGGTCTGGATCCTTGTGCCAGAGATGAAAGGGGACAGACTCCATACATGGTAGCGAACGAAAAGGAGGTCAGAAATACATTTAGACGTTTCATGGCCTCAAATCTAGATAAGTGGGACTGGAATGCTGCTAAGGTACCTAGTGCATTGACAAAGGAGATGGAGGAATCTCAGGCTGCTAAACAGGTAATTATTTGTGTGCCCCTCCACCTGTTAATTAATATAAAAAATGAAATTGTATTCTTGTTGTATTCAGGAGTTGACAGGCTCTACTTCAATAGAGAAATTTGTTCACTCATCCCATTCTATTTCTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

547

Amino Acids

60.83

Weight (kDa)

5.98

Isoelectric Point (pI)

54.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bVLRF1 PF18826 221 - 356 1.5e-46 Bacteroidetes VLRF1 release factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 422
AatII GACGTC 1 cut(s) 401
Acc36I ACCTGC 1 cut(s) 269
Acc65I GGTACC 1 cut(s) 1459
AccB1I GGYRCC 2 cut(s) 239, 1459
AccBSI CCGCTC 1 cut(s) 228
AccII CGCG 1 cut(s) 110
AciI CCGC 6 cut(s) 19, 45, 177, 226, 237, 1036
AclWI GGATC 3 cut(s) 448, 1328, 1341
AcoI YGGCCR 1 cut(s) 87
AcsI RAATTY 1 cut(s) 1608
AcuI CTGAAG 1 cut(s) 506
AcyI GRCGYC 3 cut(s) 5, 240, 398
AfaI GTAC 5 cut(s) 649, 1183, 1201, 1284, 1461
AfeI AGCGCT 1 cut(s) 305
AfiI CCNNNNNNNGG 1 cut(s) 526
AhlI ACTAGT 1 cut(s) 1202
AjiI CACGTC 1 cut(s) 36
AjnI CCWGG 1 cut(s) 294
Alw26I GTCTC 2 cut(s) 430, 1006
AlwI GGATC 3 cut(s) 448, 1328, 1341
AlwNI CAGNNNCTG 1 cut(s) 1330
Ama87I CYCGRG 1 cut(s) 427
Aor51HI AGCGCT 1 cut(s) 305
AoxI GGCC 6 cut(s) 87, 127, 153, 470, 756, 1419
ApeKI GCWGC 5 cut(s) 21, 47, 875, 1451, 1499
ApoI RAATTY 1 cut(s) 1608
ArsI GACNNNNNNTTYG 2 cut(s) 1377, 1409
AseI ATTAAT 1 cut(s) 1542
Asp700I GAANNNNTTC 1 cut(s) 509
Asp718I GGTACC 1 cut(s) 1459
AspLEI GCGC 4 cut(s) 110, 112, 242, 306
AspS9I GGNCC 4 cut(s) 298, 470, 575, 756
AsuC2I CCSGG 3 cut(s) 86, 358, 1033
AsuHPI GGTGA 1 cut(s) 320
AsuII TTCGAA 1 cut(s) 78
AvaI CYCGRG 1 cut(s) 427
AvaII GGWCC 2 cut(s) 298, 575
BaeGI GKGCMC 2 cut(s) 687, 1527
BamHI GGATCC 1 cut(s) 1333
BanI GGYRCC 2 cut(s) 239, 1459
BbvI GCAGC 5 cut(s) 33, 59, 862, 1438, 1486
BccI CCATC 3 cut(s) 80, 701, 1477
BceAI ACGGC 1 cut(s) 227
BciT130I CCWGG 1 cut(s) 296
BcnI CCSGG 3 cut(s) 86, 358, 1033
BcoDI GTCTC 2 cut(s) 430, 1006
BcuI ACTAGT 1 cut(s) 1202
BfaI CTAG 9 cut(s) 191, 639, 795, 930, 1119, 1179, 1203, 1430, 1464
BfoI RGCGCY 2 cut(s) 243, 307
BfuAI ACCTGC 1 cut(s) 269
BisI GCNGC 6 cut(s) 22, 48, 238, 876, 1452, 1500
BlpI GCTNAGC 1 cut(s) 347
BlsI GCNGC 6 cut(s) 23, 49, 239, 877, 1453, 1501
BmcAI AGTACT 1 cut(s) 1201
Bme1390I CCNGG 4 cut(s) 86, 296, 358, 1033
Bme18I GGWCC 2 cut(s) 298, 575
BmeT110I CYCGRG 1 cut(s) 427
BmgBI CACGTC 1 cut(s) 36
BmgT120I GGNCC 4 cut(s) 298, 470, 575, 756
BmiI GGNNCC 3 cut(s) 241, 1335, 1461
BmrFI CCNGG 4 cut(s) 86, 296, 358, 1033
BmsI GCATC 4 cut(s) 500, 778, 800, 886
BpmI CTGGAG 2 cut(s) 278, 834
Bpu10I CCTNAGC 1 cut(s) 1455
Bpu1102I GCTNAGC 1 cut(s) 347
Bpu14I TTCGAA 1 cut(s) 78
BpuEI CTTGAG 3 cut(s) 1132, 1180, 1286
BpuMI CCSGG 3 cut(s) 86, 358, 1033
BsaHI GRCGYC 3 cut(s) 5, 240, 398
BsaI GGTCTC 1 cut(s) 1006
BsaXI ACNNNNNCTCC 2 cut(s) 522, 552
Bsc4I CCNNNNNNNGG 1 cut(s) 526
Bse1I ACTGG 1 cut(s) 1448
BseBI CCWGG 1 cut(s) 296
BseGI GGATG 3 cut(s) 38, 513, 1620
BseLI CCNNNNNNNGG 1 cut(s) 526
BseMII CTCAG 4 cut(s) 147, 198, 270, 1508
BseNI ACTGG 1 cut(s) 1448
BsePI GCGCGC 1 cut(s) 108
BseRI GAGGAG 3 cut(s) 104, 392, 1091
BseSI GKGCMC 2 cut(s) 687, 1527
BseXI GCAGC 5 cut(s) 33, 59, 862, 1438, 1486
Bsh1236I CGCG 1 cut(s) 110
BshFI GGCC 6 cut(s) 89, 129, 155, 472, 758, 1421
BshNI GGYRCC 2 cut(s) 239, 1459
BsiHKCI CYCGRG 1 cut(s) 427
BsiSI CCGG 4 cut(s) 86, 126, 357, 1033
BslFI GGGAC 3 cut(s) 274, 1371, 1454
BslI CCNNNNNNNGG 1 cut(s) 526
BsmAI GTCTC 2 cut(s) 430, 1006
BsmFI GGGAC 3 cut(s) 274, 1371, 1454
BsmI GAATGC 2 cut(s) 1300, 1453
BsnI GGCC 6 cut(s) 89, 129, 155, 472, 758, 1421
Bso31I GGTCTC 1 cut(s) 1006
BsoBI CYCGRG 1 cut(s) 427
Bsp119I TTCGAA 1 cut(s) 78
Bsp1286I GDGCHC 2 cut(s) 687, 1527
Bsp143I GATC 3 cut(s) 217, 440, 1333
Bsp1720I GCTNAGC 1 cut(s) 347
BspACI CCGC 6 cut(s) 19, 45, 177, 226, 237, 1036
BspANI GGCC 6 cut(s) 89, 129, 155, 472, 758, 1421
BspCNI CTCAG 4 cut(s) 146, 199, 271, 1507
BspFNI CGCG 1 cut(s) 110
BspHI TCATGA 1 cut(s) 631
BspLI GGNNCC 3 cut(s) 241, 1335, 1461
BspMI ACCTGC 1 cut(s) 269
BspPI GGATC 3 cut(s) 448, 1328, 1341
BspT104I TTCGAA 1 cut(s) 78
BspT107I GGYRCC 2 cut(s) 239, 1459
BspTNI GGTCTC 1 cut(s) 1006
BsrBI CCGCTC 1 cut(s) 228
BsrI ACTGG 1 cut(s) 1448
BssHII GCGCGC 1 cut(s) 108
BssMI GATC 3 cut(s) 217, 440, 1333
BssNI GRCGYC 3 cut(s) 5, 240, 398
Bst2UI CCWGG 1 cut(s) 296
Bst4CI ACNGT 4 cut(s) 68, 777, 1050, 1287
Bst6I CTCTTC 1 cut(s) 828
BstACI GRCGYC 3 cut(s) 5, 240, 398
BstAPI GCANNNNNTGC 1 cut(s) 270
BstBI TTCGAA 1 cut(s) 78
BstC8I GCNNGC 3 cut(s) 110, 672, 1192
BstDEI CTNAG 6 cut(s) 133, 207, 279, 347, 1455, 1494
BstF5I GGATG 3 cut(s) 38, 513, 1620
BstFNI CGCG 1 cut(s) 110
BstH2I RGCGCY 2 cut(s) 243, 307
BstHHI GCGC 4 cut(s) 110, 112, 242, 306
BstKTI GATC 3 cut(s) 220, 443, 1336
BstMAI GTCTC 2 cut(s) 430, 1006
BstMBI GATC 3 cut(s) 217, 440, 1333
BstMWI GCNNNNNNNGC 5 cut(s) 234, 270, 478, 809, 1094
BstNI CCWGG 1 cut(s) 296
BstSCI CCNGG 4 cut(s) 84, 294, 356, 1031
BstSLI GKGCMC 2 cut(s) 687, 1527
BstUI CGCG 1 cut(s) 110
BstV1I GCAGC 5 cut(s) 33, 59, 862, 1438, 1486
BstX2I RGATCY 1 cut(s) 1333
BstYI RGATCY 1 cut(s) 1333
BsuRI GGCC 6 cut(s) 89, 129, 155, 472, 758, 1421
BtrI CACGTC 1 cut(s) 36
BtsCI GGATG 3 cut(s) 38, 513, 1620
BtsI GCAGTG 1 cut(s) 27
BtsIMutI CAGTG 1 cut(s) 27
BveI ACCTGC 1 cut(s) 269
Cac8I GCNNGC 3 cut(s) 110, 672, 1192
CaiI CAGNNNCTG 1 cut(s) 1330
CciI TCATGA 1 cut(s) 631
CfoI GCGC 4 cut(s) 110, 112, 242, 306
Cfr13I GGNCC 4 cut(s) 298, 470, 575, 756
Csp6I GTAC 5 cut(s) 648, 1182, 1200, 1283, 1460
CviAII CATG 6 cut(s) 594, 632, 1153, 1274, 1372, 1417
CviQI GTAC 5 cut(s) 648, 1182, 1200, 1283, 1460
DdeI CTNAG 6 cut(s) 133, 207, 279, 347, 1455, 1494
DinI GGCGCC 1 cut(s) 241
DpnI GATC 3 cut(s) 219, 442, 1335
DpnII GATC 3 cut(s) 217, 440, 1333
DraI TTTAAA 1 cut(s) 412
DrdI GACNNNNNNGTC 1 cut(s) 422
DseDI GACNNNNNNGTC 1 cut(s) 422
EaeI YGGCCR 1 cut(s) 87
Eam1104I CTCTTC 1 cut(s) 828
EarI CTCTTC 1 cut(s) 828
EciI GGCGGA 2 cut(s) 166, 1051
Eco147I AGGCCT 1 cut(s) 155
Eco31I GGTCTC 1 cut(s) 1006
Eco32I GATATC 1 cut(s) 1141
Eco47I GGWCC 2 cut(s) 298, 575
Eco47III AGCGCT 1 cut(s) 305
Eco57I CTGAAG 1 cut(s) 506
Eco88I CYCGRG 1 cut(s) 427
EcoRII CCWGG 1 cut(s) 294
EcoRV GATATC 1 cut(s) 1141
EcoT22I ATGCAT 1 cut(s) 793
EgeI GGCGCC 1 cut(s) 241
EheI GGCGCC 1 cut(s) 241
FaeI CATG 6 cut(s) 597, 635, 1156, 1277, 1375, 1420
FalI AAGNNNNNCTT 4 cut(s) 855, 887, 875, 907
FaqI GGGAC 3 cut(s) 274, 1371, 1454
FatI CATG 6 cut(s) 593, 631, 1152, 1273, 1371, 1416
FauI CCCGC 1 cut(s) 26
Fnu4HI GCNGC 6 cut(s) 22, 48, 238, 876, 1452, 1500
FokI GGATG 3 cut(s) 25, 500, 1607
Fsp4HI GCNGC 6 cut(s) 22, 48, 238, 876, 1452, 1500
FspBI CTAG 9 cut(s) 191, 639, 795, 930, 1119, 1179, 1203, 1430, 1464
GlaI GCGC 4 cut(s) 109, 111, 241, 305
GluI GCNGC 6 cut(s) 22, 48, 238, 876, 1452, 1500
GsuI CTGGAG 2 cut(s) 278, 834
HaeII RGCGCY 2 cut(s) 243, 307
HaeIII GGCC 6 cut(s) 89, 129, 155, 472, 758, 1421
HapII CCGG 4 cut(s) 86, 126, 357, 1033
HhaI GCGC 4 cut(s) 110, 112, 242, 306
Hin1I GRCGYC 3 cut(s) 5, 240, 398
Hin1II CATG 6 cut(s) 597, 635, 1156, 1277, 1375, 1420
Hin6I GCGC 4 cut(s) 108, 110, 240, 304
HinP1I GCGC 4 cut(s) 108, 110, 240, 304
HincII GTYRAC 4 cut(s) 1053, 1165, 1263, 1585
HindII GTYRAC 4 cut(s) 1053, 1165, 1263, 1585
HindIII AAGCTT 2 cut(s) 479, 1188
HpaII CCGG 4 cut(s) 86, 126, 357, 1033
HphI GGTGA 1 cut(s) 320
Hpy166II GTNNAC 5 cut(s) 1053, 1165, 1263, 1585, 1616
Hpy188I TCNGA 7 cut(s) 10, 322, 403, 445, 525, 1011, 1397
Hpy8I GTNNAC 5 cut(s) 1053, 1165, 1263, 1585, 1616
Hpy99I CGWCG 1 cut(s) 831
HpyAV CCTTC 6 cut(s) 157, 367, 530, 745, 936, 1018
HpyCH4III ACNGT 4 cut(s) 68, 777, 1050, 1287
HpyCH4IV ACGT 3 cut(s) 35, 398, 1411
HpyCH4V TGCA 8 cut(s) 264, 273, 497, 674, 791, 845, 1273, 1469
HpyF10VI GCNNNNNNNGC 5 cut(s) 234, 270, 478, 809, 1094
HpyF3I CTNAG 6 cut(s) 133, 207, 279, 347, 1455, 1494
HpySE526I ACGT 3 cut(s) 35, 398, 1411
Hsp92I GRCGYC 3 cut(s) 5, 240, 398
Hsp92II CATG 6 cut(s) 597, 635, 1156, 1277, 1375, 1420
HspAI GCGC 4 cut(s) 108, 110, 240, 304
KasI GGCGCC 1 cut(s) 239
KpnI GGTACC 1 cut(s) 1463
Kzo9I GATC 3 cut(s) 217, 440, 1333
LmnI GCTCC 5 cut(s) 117, 179, 233, 815, 1093
Lsp1109I GCAGC 5 cut(s) 33, 59, 862, 1438, 1486
LweI GCATC 4 cut(s) 500, 778, 800, 886
MaeI CTAG 9 cut(s) 191, 639, 795, 930, 1119, 1179, 1203, 1430, 1464
MaeII ACGT 3 cut(s) 35, 398, 1411
MaeIII GTNAC 1 cut(s) 710
MalI GATC 3 cut(s) 219, 442, 1335
MbiI CCGCTC 1 cut(s) 228
MboI GATC 3 cut(s) 217, 440, 1333
MboII GAAGA 4 cut(s) 67, 88, 373, 815
MflI RGATCY 1 cut(s) 1333
MhlI GDGCHC 2 cut(s) 687, 1527
MluCI AATT 6 cut(s) 506, 602, 1512, 1539, 1557, 1608
Mly113I GGCGCC 1 cut(s) 240
MlyI GAGTC 6 cut(s) 95, 206, 244, 296, 1217, 1357
Mph1103I ATGCAT 1 cut(s) 793
MroXI GAANNNNTTC 1 cut(s) 509
MseI TTAA 4 cut(s) 411, 1251, 1538, 1542
MslI CAYNNNNRTG 1 cut(s) 744
MspI CCGG 4 cut(s) 86, 126, 357, 1033
MspR9I CCNGG 4 cut(s) 86, 296, 358, 1033
Mva1269I GAATGC 2 cut(s) 1300, 1453
MvaI CCWGG 1 cut(s) 296
MvnI CGCG 1 cut(s) 110
MwoI GCNNNNNNNGC 5 cut(s) 234, 270, 478, 809, 1094
NarI GGCGCC 1 cut(s) 240
NciI CCSGG 3 cut(s) 86, 358, 1033
NdeII GATC 3 cut(s) 217, 440, 1333
NlaIII CATG 6 cut(s) 597, 635, 1156, 1277, 1375, 1420
NlaIV GGNNCC 3 cut(s) 241, 1335, 1461
NmeAIII GCCGAG 1 cut(s) 232
NsiI ATGCAT 1 cut(s) 793
NspV TTCGAA 1 cut(s) 78
PacI TTAATTAA 1 cut(s) 1542
PaeR7I CTCGAG 1 cut(s) 427
PagI TCATGA 1 cut(s) 631
PauI GCGCGC 1 cut(s) 108
PceI AGGCCT 1 cut(s) 155
PctI GAATGC 2 cut(s) 1300, 1453
PdmI GAANNNNTTC 1 cut(s) 509
PfeI GAWTC 4 cut(s) 333, 404, 569, 1490
PfoI TCCNGGA 1 cut(s) 294
PkrI GCNGC 6 cut(s) 23, 49, 239, 877, 1453, 1501
PleI GAGTC 6 cut(s) 95, 205, 244, 295, 1216, 1357
PluTI GGCGCC 1 cut(s) 243
PpsI GAGTC 6 cut(s) 95, 205, 244, 295, 1216, 1357
PshBI ATTAAT 1 cut(s) 1542
Psp6I CCWGG 1 cut(s) 294
PspGI CCWGG 1 cut(s) 294
PspN4I GGNNCC 3 cut(s) 241, 1335, 1461
PspPI GGNCC 4 cut(s) 298, 470, 575, 756
PstNI CAGNNNCTG 1 cut(s) 1330
PsuI RGATCY 1 cut(s) 1333
PteI GCGCGC 1 cut(s) 108
RsaI GTAC 5 cut(s) 649, 1183, 1201, 1284, 1461
RsaNI GTAC 5 cut(s) 648, 1182, 1200, 1283, 1460
RseI CAYNNNNRTG 1 cut(s) 744
SaqAI TTAA 4 cut(s) 411, 1251, 1538, 1542
SatI GCNGC 6 cut(s) 22, 48, 238, 876, 1452, 1500
Sau3AI GATC 3 cut(s) 217, 440, 1333
Sau96I GGNCC 4 cut(s) 298, 470, 575, 756
ScaI AGTACT 1 cut(s) 1201
SchI GAGTC 6 cut(s) 95, 206, 244, 296, 1217, 1357
ScrFI CCNGG 4 cut(s) 86, 296, 358, 1033
SduI GDGCHC 2 cut(s) 687, 1527
SfaNI GCATC 4 cut(s) 500, 778, 800, 886
SfoI GGCGCC 1 cut(s) 241
Sfr274I CTCGAG 1 cut(s) 427
SfuI TTCGAA 1 cut(s) 78
SinI GGWCC 2 cut(s) 298, 575
SlaI CTCGAG 1 cut(s) 427
SmiMI CAYNNNNRTG 1 cut(s) 744
SmlI CTYRAG 4 cut(s) 427, 1147, 1195, 1301
SmoI CTYRAG 4 cut(s) 427, 1147, 1195, 1301
SpeI ACTAGT 1 cut(s) 1202
Sse9I AATT 6 cut(s) 506, 602, 1512, 1539, 1557, 1608
SseBI AGGCCT 1 cut(s) 155
SsiI CCGC 6 cut(s) 19, 45, 177, 226, 237, 1036
SspDI GGCGCC 1 cut(s) 239
SspI AATATT 2 cut(s) 367, 994
SspMI CTAG 9 cut(s) 191, 639, 795, 930, 1119, 1179, 1203, 1430, 1464
StuI AGGCCT 1 cut(s) 155
StyD4I CCNGG 4 cut(s) 84, 294, 356, 1031
TaaI ACNGT 4 cut(s) 68, 777, 1050, 1287
TaiI ACGT 3 cut(s) 38, 401, 1414
TaqI TCGA 8 cut(s) 78, 99, 150, 183, 248, 285, 428, 894
TasI AATT 6 cut(s) 506, 602, 1512, 1539, 1557, 1608
TatI WGTACW 3 cut(s) 1181, 1199, 1282
TauI GCSGC 1 cut(s) 240
TfiI GAWTC 4 cut(s) 333, 404, 569, 1490
Tru1I TTAA 4 cut(s) 411, 1251, 1538, 1542
Tru9I TTAA 4 cut(s) 411, 1251, 1538, 1542
TscAI CASTG 1 cut(s) 34
TseI GCWGC 5 cut(s) 21, 47, 875, 1451, 1499
TspRI CASTG 1 cut(s) 34
VpaK11BI GGWCC 2 cut(s) 298, 575
VspI ATTAAT 1 cut(s) 1542
XapI RAATTY 1 cut(s) 1608
XbaI TCTAGA 1 cut(s) 1429
XhoI CTCGAG 1 cut(s) 427
XmnI GAANNNNTTC 1 cut(s) 509
XspI CTAG 9 cut(s) 191, 639, 795, 930, 1119, 1179, 1203, 1430, 1464
ZraI GACGTC 1 cut(s) 399
ZrmI AGTACT 1 cut(s) 1201
Zsp2I ATGCAT 1 cut(s) 793
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.