Rh1DG227000

Ankyrin repeat and zinc finger domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0007214
Physical Location & Seq
Reverse (-)
43728202 .. 43732642
4441 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG227000.1

Sequence Viewer

Length: 1392 bp
ATGGCGTCGGAGCCTCCTCGCACCACCACGTCATCCACCACGACCGCCGCCGAAAAAGAGAAACGACACCGTTCCATCTTCGAACTCCCGGCCAACTTCTTCGACTCCTGCGCCCTCCTCACTTCTTCCGGCCTCTCAGTTGCTTCAACTTTCGAGACCTCTAACAACAGCAACAACTCCGCCCTCGAAACCCTAGACGAGTCTGTGTCCGATAACACCGATCAAGACCGCTCCCTAAACGCCGCCGTATCGACTCGGTGGACCTGCAACACTTGCAAGGCTGAGTTCGAGTCCCTCCAAGAGCAGCAATTTCACTTCAAATCCGATATTCACCGATTCAATGTGAAGCTAAGCCTTGCTGGAAAGAATATTGTGAAGGAGGAGGACTTTGATGAGTTAACGTCTGATTCTTTTAAAGACTTTGATGTCTCGAGTATATCGGGATCAGAGGATGATGAACCTGAAAAAGTTGGCCCTTTCAAGCTTTCAAGGGCAACTGCTAGGCAGAAATTACTCATCCACCTTCAGACGGGAGAAAGAATTTCGATTTGGAAGTGTTTGATTACAAATGAATCTGGACCTGAAGGTAATTTGTCGTGTGATGAATTGTTGGGGAGATTGAACACGGTGGTTCATGAGCCTAGAGATAATACCTGTTTTAGAGTTGTGTTGCTTGCAAGTGGTGGGCACTTTGCTGGTTGTGTGTTTGATGGTAGCAAGATTGTAGCTCACAAGACTTTCCACAGGTATGTCGTAAGGGCCAAGTCTGGAAAAAAACAGTCATCCAATGATGCATCTGGAAGGTTTGCTCATTCCGCTGGAGCTTCTCTTCGTCGCCATAATGAACTTGCATTGAAAAAGGAATTGCCGTCTAGTTCAGGAATTGAAAATGATATCATCTCAAGCATGGTGAAAGTCAACATTGTTGAGCCTTTTGTTGAGCCTAGTACAGAAGCTTGCTCAAGTATTAGAGAGTCCAATGAACTCCCTATATCAAGTGAGAGTAGTAATGAGGTTAAAGGTTCGTCAACACCTTTGCATGAGGCAGCAGAGTCTGGGAATGCTCTCAAGGTTTTGGAACTCTTGGAACAGGGTCTGGATCCTTGTGCCAGAGATGAAAGGGGACAGACTCCATACACGGCTGAGGCTGCTGCTTCCAAGAATACTTCGACCATTACAAAGTCCTCTTCATCATCTCAGCCCAGTCGTGCATCACAGATCTCTAAAGAGGAGGAACTGAAAAGGGCACTGGTAGCCGAGAGGGAAAAACGAGCAGCCGCAGCCGAGCGGAGAATGGCTGCAGCTGCAGCCCTTAGTGCTCAAGTAATTGAAGCAAATGGCAATAGTAGGAATATTTGTGACAATGAGAATGTAGATGTGCTCCGACTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

463

Amino Acids

50.37

Weight (kDa)

5.55

Isoelectric Point (pI)

50.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bVLRF1 PF18826 222 - 289 3.1e-25 Bacteroidetes VLRF1 release factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 425
Acc36I ACCTGC 1 cut(s) 272
AccBSI CCGCTC 2 cut(s) 231, 1288
AciI CCGC 8 cut(s) 45, 48, 180, 229, 243, 816, 1278, 1288
AclWI GGATC 3 cut(s) 451, 1094, 1107
AcoI YGGCCR 1 cut(s) 90
AcsI RAATTY 1 cut(s) 540
AcuI CTGAAG 2 cut(s) 509, 603
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 949
AfiI CCNNNNNNNGG 1 cut(s) 529
AgsI TTSAA 9 cut(s) 147, 319, 340, 481, 489, 622, 856, 887, 1331
AjiI CACGTC 1 cut(s) 30
AjuI GAANNNNNNNTTGG 2 cut(s) 532, 564
AluBI AGCT 6 cut(s) 349, 484, 728, 824, 956, 1304
AluI AGCT 6 cut(s) 349, 484, 728, 824, 956, 1304
Alw21I GWGCWC 2 cut(s) 1321, 1383
Alw26I GTCTC 2 cut(s) 149, 433
AlwI GGATC 3 cut(s) 451, 1094, 1107
AlwNI CAGNNNCTG 2 cut(s) 1055, 1096
Ama87I CYCGRG 1 cut(s) 430
AoxI GGCC 4 cut(s) 90, 130, 472, 759
ApoI RAATTY 1 cut(s) 540
AspLEI GCGC 1 cut(s) 113
AspS9I GGNCC 4 cut(s) 261, 473, 578, 759
AsuC2I CCSGG 1 cut(s) 89
AsuHPI GGTGA 2 cut(s) 323, 922
AsuII TTCGAA 1 cut(s) 81
AvaI CYCGRG 1 cut(s) 430
AvaII GGWCC 2 cut(s) 261, 578
BaeGI GKGCMC 2 cut(s) 690, 1249
BamHI GGATCC 1 cut(s) 1099
Bbv12I GWGCWC 2 cut(s) 1321, 1383
BbvCI CCTCAGC 1 cut(s) 1143
BccI CCATC 2 cut(s) 83, 704
BceAI ACGGC 3 cut(s) 230, 853, 1155
BcnI CCSGG 1 cut(s) 89
BcoDI GTCTC 2 cut(s) 149, 433
BfaI CTAG 5 cut(s) 194, 501, 642, 873, 945
BfmI CTRYAG 3 cut(s) 1299, 1305, 1388
BfuAI ACCTGC 1 cut(s) 272
BglII AGATCT 1 cut(s) 1218
BlpI GCTNAGC 1 cut(s) 350
Bme1390I CCNGG 1 cut(s) 89
Bme18I GGWCC 2 cut(s) 261, 578
BmeT110I CYCGRG 1 cut(s) 430
BmgBI CACGTC 1 cut(s) 30
BmgT120I GGNCC 4 cut(s) 261, 473, 578, 759
BmiI GGNNCC 2 cut(s) 12, 1101
BmrFI CCNGG 1 cut(s) 89
BmrI ACTGGG 1 cut(s) 1197
BmsI GCATC 3 cut(s) 781, 803, 1220
BmuI ACTGGG 1 cut(s) 1197
BpmI CTGGAG 1 cut(s) 840
Bpu10I CCTNAGC 1 cut(s) 1143
Bpu1102I GCTNAGC 1 cut(s) 350
Bpu14I TTCGAA 1 cut(s) 81
BpuEI CTTGAG 4 cut(s) 886, 946, 1052, 1305
BpuMI CCSGG 1 cut(s) 89
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 149
Bsc4I CCNNNNNNNGG 1 cut(s) 529
Bse1I ACTGG 2 cut(s) 1203, 1254
BseGI GGATG 4 cut(s) 32, 457, 516, 782
BseLI CCNNNNNNNGG 1 cut(s) 529
BseMII CTCAG 4 cut(s) 150, 273, 1134, 1211
BseNI ACTGG 2 cut(s) 1203, 1254
BseRI GAGGAG 4 cut(s) 6, 107, 395, 1244
BseSI GKGCMC 2 cut(s) 690, 1249
Bsh1285I CGRYCG 1 cut(s) 45
BshFI GGCC 4 cut(s) 92, 132, 474, 761
BsiEI CGRYCG 1 cut(s) 45
BsiHKAI GWGCWC 2 cut(s) 1321, 1383
BsiHKCI CYCGRG 1 cut(s) 430
BsiSI CCGG 2 cut(s) 89, 129
BslFI GGGAC 2 cut(s) 277, 1137
BslI CCNNNNNNNGG 1 cut(s) 529
BsmAI GTCTC 2 cut(s) 149, 433
BsmFI GGGAC 2 cut(s) 277, 1137
BsmI GAATGC 1 cut(s) 1066
BsnI GGCC 4 cut(s) 92, 132, 474, 761
Bso31I GGTCTC 1 cut(s) 149
BsoBI CYCGRG 1 cut(s) 430
Bsp119I TTCGAA 1 cut(s) 81
Bsp1286I GDGCHC 4 cut(s) 690, 1249, 1321, 1383
Bsp143I GATC 4 cut(s) 220, 443, 1099, 1218
Bsp1720I GCTNAGC 1 cut(s) 350
BspACI CCGC 8 cut(s) 45, 48, 180, 229, 243, 816, 1278, 1288
BspANI GGCC 4 cut(s) 92, 132, 474, 761
BspCNI CTCAG 4 cut(s) 149, 274, 1135, 1210
BspHI TCATGA 1 cut(s) 634
BspLI GGNNCC 2 cut(s) 12, 1101
BspMAI CTGCAG 2 cut(s) 1303, 1309
BspMI ACCTGC 1 cut(s) 272
BspPI GGATC 3 cut(s) 451, 1094, 1107
BspT104I TTCGAA 1 cut(s) 81
BspTNI GGTCTC 1 cut(s) 149
BsrBI CCGCTC 2 cut(s) 231, 1288
BsrI ACTGG 2 cut(s) 1203, 1254
BssMI GATC 4 cut(s) 220, 443, 1099, 1218
BssNI GRCGYC 1 cut(s) 5
Bst4CI ACNGT 4 cut(s) 71, 628, 780, 1389
Bst6I CTCTTC 2 cut(s) 834, 1192
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 273
BstBI TTCGAA 1 cut(s) 81
BstC8I GCNNGC 2 cut(s) 675, 958
BstDEI CTNAG 6 cut(s) 136, 282, 350, 1143, 1197, 1313
BstF5I GGATG 4 cut(s) 32, 457, 516, 782
BstHHI GCGC 1 cut(s) 113
BstKTI GATC 4 cut(s) 223, 446, 1102, 1221
BstMAI GTCTC 2 cut(s) 149, 433
BstMBI GATC 4 cut(s) 220, 443, 1099, 1218
BstMCI CGRYCG 1 cut(s) 45
BstMWI GCNNNNNNNGC 8 cut(s) 273, 815, 1148, 1253, 1280, 1304, 1307, 1316
BstSCI CCNGG 1 cut(s) 87
BstSFI CTRYAG 3 cut(s) 1299, 1305, 1388
BstSLI GKGCMC 2 cut(s) 690, 1249
BstX2I RGATCY 2 cut(s) 1099, 1218
BstYI RGATCY 2 cut(s) 1099, 1218
BsuRI GGCC 4 cut(s) 92, 132, 474, 761
BtrI CACGTC 1 cut(s) 30
BtsCI GGATG 4 cut(s) 32, 457, 516, 782
BtsIMutI CAGTG 1 cut(s) 1247
BveI ACCTGC 1 cut(s) 272
Cac8I GCNNGC 2 cut(s) 675, 958
CaiI CAGNNNCTG 2 cut(s) 1055, 1096
CciI TCATGA 1 cut(s) 634
CfoI GCGC 1 cut(s) 113
Cfr13I GGNCC 4 cut(s) 261, 473, 578, 759
Csp6I GTAC 1 cut(s) 948
CviAII CATG 3 cut(s) 635, 907, 1040
CviQI GTAC 1 cut(s) 948
DdeI CTNAG 6 cut(s) 136, 282, 350, 1143, 1197, 1313
DpnI GATC 4 cut(s) 222, 445, 1101, 1220
DpnII GATC 4 cut(s) 220, 443, 1099, 1218
DraI TTTAAA 1 cut(s) 415
DrdI GACNNNNNNGTC 1 cut(s) 425
DseDI GACNNNNNNGTC 1 cut(s) 425
EaeI YGGCCR 1 cut(s) 90
Eam1104I CTCTTC 2 cut(s) 834, 1192
EarI CTCTTC 2 cut(s) 834, 1192
EciI GGCGGA 1 cut(s) 169
Eco31I GGTCTC 1 cut(s) 149
Eco32I GATATC 1 cut(s) 895
Eco47I GGWCC 2 cut(s) 261, 578
Eco57I CTGAAG 2 cut(s) 509, 603
Eco88I CYCGRG 1 cut(s) 430
EcoRV GATATC 1 cut(s) 895
EcoT22I ATGCAT 1 cut(s) 796
FaeI CATG 3 cut(s) 638, 910, 1043
FaiI YATR 8 cut(s) 437, 636, 750, 840, 908, 992, 1041, 1135
FaqI GGGAC 2 cut(s) 277, 1137
FatI CATG 3 cut(s) 634, 906, 1039
FokI GGATG 4 cut(s) 19, 464, 503, 769
FspBI CTAG 5 cut(s) 194, 501, 642, 873, 945
GlaI GCGC 1 cut(s) 112
GsuI CTGGAG 1 cut(s) 840
HaeIII GGCC 4 cut(s) 92, 132, 474, 761
HapII CCGG 2 cut(s) 89, 129
HhaI GCGC 1 cut(s) 113
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 3 cut(s) 638, 910, 1043
Hin6I GCGC 1 cut(s) 111
HinP1I GCGC 1 cut(s) 111
HincII GTYRAC 3 cut(s) 399, 919, 1029
HindII GTYRAC 3 cut(s) 399, 919, 1029
HindIII AAGCTT 2 cut(s) 482, 954
HpaI GTTAAC 1 cut(s) 399
HpaII CCGG 2 cut(s) 89, 129
HphI GGTGA 2 cut(s) 323, 922
Hpy166II GTNNAC 4 cut(s) 261, 399, 919, 1029
Hpy188I TCNGA 7 cut(s) 10, 211, 325, 406, 448, 528, 1385
Hpy8I GTNNAC 4 cut(s) 261, 399, 919, 1029
Hpy99I CGWCG 2 cut(s) 10, 837
HpyAV CCTTC 4 cut(s) 370, 533, 578, 795
HpyCH4III ACNGT 4 cut(s) 71, 628, 780, 1389
HpyCH4IV ACGT 2 cut(s) 29, 401
HpyCH4V TGCA 9 cut(s) 267, 276, 677, 794, 851, 1039, 1211, 1301, 1307
HpyF10VI GCNNNNNNNGC 8 cut(s) 273, 815, 1148, 1253, 1280, 1304, 1307, 1316
HpyF3I CTNAG 6 cut(s) 136, 282, 350, 1143, 1197, 1313
HpySE526I ACGT 2 cut(s) 29, 401
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 3 cut(s) 638, 910, 1043
HspAI GCGC 1 cut(s) 111
KspAI GTTAAC 1 cut(s) 399
Kzo9I GATC 4 cut(s) 220, 443, 1099, 1218
LmnI GCTCC 4 cut(s) 10, 236, 821, 1386
LweI GCATC 3 cut(s) 781, 803, 1220
MaeI CTAG 5 cut(s) 194, 501, 642, 873, 945
MaeII ACGT 2 cut(s) 29, 401
MaeIII GTNAC 1 cut(s) 1358
MalI GATC 4 cut(s) 222, 445, 1101, 1220
MbiI CCGCTC 2 cut(s) 231, 1288
MboI GATC 4 cut(s) 220, 443, 1099, 1218
MboII GAAGA 5 cut(s) 70, 91, 117, 821, 1179
MflI RGATCY 2 cut(s) 1099, 1218
MhlI GDGCHC 4 cut(s) 690, 1249, 1321, 1383
MluCI AATT 8 cut(s) 308, 509, 540, 589, 605, 863, 882, 1326
MlyI GAGTC 7 cut(s) 98, 209, 247, 299, 983, 1061, 1123
Mph1103I ATGCAT 1 cut(s) 796
MseI TTAA 3 cut(s) 398, 414, 1017
MslI CAYNNNNRTG 1 cut(s) 747
MspA1I CMGCKG 2 cut(s) 818, 1304
MspI CCGG 2 cut(s) 89, 129
MspR9I CCNGG 1 cut(s) 89
Mva1269I GAATGC 1 cut(s) 1066
MwoI GCNNNNNNNGC 8 cut(s) 273, 815, 1148, 1253, 1280, 1304, 1307, 1316
NciI CCSGG 1 cut(s) 89
NdeII GATC 4 cut(s) 220, 443, 1099, 1218
NlaIII CATG 3 cut(s) 638, 910, 1043
NlaIV GGNNCC 2 cut(s) 12, 1101
NmeAIII GCCGAG 2 cut(s) 1282, 1309
NmuCI GTSAC 1 cut(s) 1358
NsiI ATGCAT 1 cut(s) 796
NspV TTCGAA 1 cut(s) 81
PaeR7I CTCGAG 1 cut(s) 430
PagI TCATGA 1 cut(s) 634
PctI GAATGC 1 cut(s) 1066
PfeI GAWTC 3 cut(s) 336, 407, 572
PleI GAGTC 7 cut(s) 98, 208, 247, 298, 982, 1060, 1123
PpsI GAGTC 7 cut(s) 98, 208, 247, 298, 982, 1060, 1123
PspN4I GGNNCC 2 cut(s) 12, 1101
PspPI GGNCC 4 cut(s) 261, 473, 578, 759
PstI CTGCAG 2 cut(s) 1303, 1309
PstNI CAGNNNCTG 2 cut(s) 1055, 1096
PsuI RGATCY 2 cut(s) 1099, 1218
PvuII CAGCTG 1 cut(s) 1304
RsaI GTAC 1 cut(s) 949
RsaNI GTAC 1 cut(s) 948
RseI CAYNNNNRTG 1 cut(s) 747
SaqAI TTAA 3 cut(s) 398, 414, 1017
Sau3AI GATC 4 cut(s) 220, 443, 1099, 1218
Sau96I GGNCC 4 cut(s) 261, 473, 578, 759
SchI GAGTC 7 cut(s) 98, 209, 247, 299, 983, 1061, 1123
ScrFI CCNGG 1 cut(s) 89
SduI GDGCHC 4 cut(s) 690, 1249, 1321, 1383
SfaNI GCATC 3 cut(s) 781, 803, 1220
SfcI CTRYAG 3 cut(s) 1299, 1305, 1388
Sfr274I CTCGAG 1 cut(s) 430
SfuI TTCGAA 1 cut(s) 81
SinI GGWCC 2 cut(s) 261, 578
SlaI CTCGAG 1 cut(s) 430
SmiMI CAYNNNNRTG 1 cut(s) 747
SmlI CTYRAG 5 cut(s) 430, 901, 961, 1067, 1320
SmoI CTYRAG 5 cut(s) 430, 901, 961, 1067, 1320
Sse9I AATT 8 cut(s) 308, 509, 540, 589, 605, 863, 882, 1326
SsiI CCGC 8 cut(s) 45, 48, 180, 229, 243, 816, 1278, 1288
SspI AATATT 2 cut(s) 370, 1354
SspMI CTAG 5 cut(s) 194, 501, 642, 873, 945
StyD4I CCNGG 1 cut(s) 87
TaaI ACNGT 4 cut(s) 71, 628, 780, 1389
TaiI ACGT 2 cut(s) 32, 404
TaqI TCGA 9 cut(s) 81, 102, 153, 186, 251, 288, 431, 545, 1169
TasI AATT 8 cut(s) 308, 509, 540, 589, 605, 863, 882, 1326
TatI WGTACW 1 cut(s) 947
TauI GCSGC 3 cut(s) 50, 245, 1280
TfiI GAWTC 3 cut(s) 336, 407, 572
Tru1I TTAA 3 cut(s) 398, 414, 1017
Tru9I TTAA 3 cut(s) 398, 414, 1017
TscAI CASTG 1 cut(s) 1254
TseFI GTSAC 1 cut(s) 1358
Tsp45I GTSAC 1 cut(s) 1358
TspDTI ATGAA 8 cut(s) 471, 585, 618, 623, 858, 996, 1131, 1179
TspRI CASTG 1 cut(s) 1254
VpaK11BI GGWCC 2 cut(s) 261, 578
XapI RAATTY 1 cut(s) 540
XhoI CTCGAG 1 cut(s) 430
XspI CTAG 5 cut(s) 194, 501, 642, 873, 945
Zsp2I ATGCAT 1 cut(s) 796
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.