Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0007214

Physical Location & Seq

Genomic Coordinates Forward (+)

19889780 .. 19894943

Gene Structure 5164 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1201200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1986 bp

ATGGCGACCGAGCCTCTTCGCACCACCGCAACCCCAGCAACTCACACGGCGGCGCTGACAAAAGAGAAACGACCCCGTTCCATCTTCGACGTCGGGGCCGACTTCTTCGACTCCTGCGAGCTCCTTCATTCTTCTGGCCTCTCTACTGCCTCAACCACCGATGCCTCCAACTACAATTCCGCCGTCGAAACCCTAGACGACTTCGAGCCAGAGCGCAGTGAACAAAACCCCTCCCAAAACGCCGTCGTAGCAGGACCCCGGTGGACCTGCAACACTTGCAAGGCCGAGTTCGACTCCCTCCAAGACCAGCGCTCGCACTTCAAATCCGACATTCACCGATTCAACGTGAAGTTAAGTGTTGCTGGAAAGAGTATTGTGAAGGAGGATGACTTTGATGAGTTAACATCCGATTCTTTTAAAGACTTTGATGTCTCAAGTATATCGGGGTCAGATGAAGATGAACCTGAAAGGGGAGTTCGCTCTTTGAAGTTTTCAAGGGAAAATATCAGGCAGAAGTTATTTTTCCACCTTCCGACTGGTGAGAGGGTTTCAGTTTGGAAGTGTTTAGTTACAAATGATTCTGGACCTGAAGGTACTTTGTCAGATGAGGAAGTGAGAGAGAGATTGAAAACGTTGGTTCATGAACCTAGGGATAAGACCCGTTTAAGAATCATGTTGCTTGCAAGTGGTGGGCACTTTGCCGGTTGTGTCTTTGATGGTAACTTGGTTGTGGATCACAAGACCTTCCACAGATATGTTGTAAGGGGCAAGTCTGGTAAAAAACAGTCATCCAAAGATGCAGGTGGCAGGTTTGCTCATTCTGCAGGAGCTTCTATGCGTCGTTATAATGAACTTGCTTTGAAAAAGGAAATTCAAGAGCTGCTTGTTGCTTGGAAGCAATACTTTGATTCTTCCTCTTGCGTTTTTGTTTATGCTCCTTCTAGCAATCATCAGCTGCTTTTTAATGGGGACAACAAACCATATTTTAGTCATCAGAATTGTGTTGTTCAAAATGTTCCAATGACTGTTCGGAGACCTACTTATAAGGAAGCTCGGCGTATATATGAGCAATTGACCCAAGTTGTTTATGAACTAGATGAAAATGTAGCTCCACCAAGTTTCAAAGAGGACCCGCCACTTACTTCAGGTATTAACAGCGACCTCATCCCAAGAATGAACAAAGTCGATATTGCTGACAGTTCAGATTGTCGAGAAAGACCTGAAGCTTGCCTAGATAGTAGAAAGTCCAATGAATTCCCTATATCAAGTGACAGTGATGGTGAGGAAATATGTTCGTCAACACCCTTGCACGAAGCAGCGCATTCTGGAAATACTCTTAAGGTTTTGGAACTATTGGAACAAGGCCTGGATCCTTGCATCAAAGATGAAAGGGGACTGACTCCATACATGCTAGCAAGTGATAAAGAGGTCAGAAACACATTTAGACGGTTCATGGCCTCAAACCCGGATAAGTGGGATTGGAATGCTGCTAAGGTACCTAGTCCATTGACAAAAGAGTTGGAGGAATCTCAAGCTGCTAAGCAGGCTGAAAAAGATGCCAAAAGGAAAGTAAGAGCAAAAGAATTGAAGAAATTACGAAAAGAAAAAGAAAAGAAAGCTCAGGCTGAGGCTGCTGCTTCCCAAAAAGCTTCGACGTCTTCTAGTCTTAAGGGAAAGCCTCAACCTAGTTCTGGATCACATAGCTCTAAAGAGGAGGAACTGAAAAGGGTGCAGGCAGCTGAGAGGGAGAAAAGAGCAGCTGCTGCGGAGAGGAGAAGAGCAGCAGCTGAAGCTCTTAGCGCTCAAGGCAATAGCAGTAGCGTTGCACCGAGCACTGTTCAGCCCAAGAGCGGGGTAGCTGGTGACATTAACTGCTCCTGTTGTAATGTATCACTGGCTGGTAAAGTTCCTTTTCATAGGTACAATTACAAATACTGCAGCACATCATGCATGCATTTGCACCGTGAGATTCTTGAGGATGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

662

Amino Acids

73.68

Weight (kDa)

7.01

Isoelectric Point (pI)

47.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
bVLRF1	PF18826	224 - 361	5.7e-46	Bacteroidetes VLRF1 release factor
VATC	PF18716	624 - 656	1.7e-11	Vms1-associating treble clef domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007214)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G01930
fragaria_vesca	FvH4_7g12110 FvH4_7g12110 FvH4_7g12110 FvH4_7g12110 FvH4_7g12110 FvH4_7g12110 FvH4_7g12110
malus_domestica	MD02G1201200.v1.1
prunus_persica	Prupe.2G147600_v2.0.a1 Prupe.2G147600_v2.0.a1
pyrus_communis	pycom02g16460
rosa_chinensis	RchiOBHm_Chr1g0350861
rosa_laevigata	RLG00000028448
rosa_multiflora	Rmu_sc0006200.1_g000008 Rmu_sc0006200.1_g000010 Rmu_sc0008251.1_g000001 Rmu_sc0014584.1_g000013
rosa_roxburghii	Rroxscaffold_4G00304460
rosa_rugosa	Rorug01G0214700
rosa_samantha	Rh1AG229100 Rh1BG199100 Rh1CG213900 Rh1DG227000
rosa_wichuraiana	Rw1G019850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	846, 1044
AarI	CACCTGC	1 cut(s)	791
AasI	GACNNNNNNGTC	1 cut(s)	428
AatII	GACGTC	2 cut(s)	93, 1658
Acc36I	ACCTGC	3 cut(s)	275, 791, 798
Acc65I	GGTACC	1 cut(s)	1495
AccB1I	GGYRCC	1 cut(s)	1495
AccBSI	CCGCTC	1 cut(s)	1851
AciI	CCGC	6 cut(s)	27, 50, 180, 1133, 1766, 1851
AclI	AACGTT	1 cut(s)	632
AclWI	GGATC	4 cut(s)	741, 1364, 1377, 1702
AcsI	RAATTY	2 cut(s)	870, 1253
AcuI	CTGAAG	4 cut(s)	609, 1128, 1242, 1809
AcyI	GRCGYC	2 cut(s)	90, 1655
AfaI	GTAC	3 cut(s)	595, 1497, 1922
AfeI	AGCGCT	2 cut(s)	311, 1801
AfiI	CCNNNNNNNGG	4 cut(s)	470, 1691, 1850, 1851
AflII	CTTAAG	2 cut(s)	1337, 1667
AjnI	CCWGG	1 cut(s)	1365
Alw21I	GWGCWC	2 cut(s)	123, 1835
Alw26I	GTCTC	2 cut(s)	436, 1027
AlwI	GGATC	4 cut(s)	741, 1364, 1377, 1702
AlwNI	CAGNNNCTG	2 cut(s)	1763, 1787
Aor51HI	AGCGCT	2 cut(s)	311, 1801
AoxI	GGCC	5 cut(s)	96, 136, 282, 1363, 1455
ApoI	RAATTY	2 cut(s)	870, 1253
Asp718I	GGTACC	1 cut(s)	1495
AspA2I	CCTAGG	1 cut(s)	647
AspLEI	GCGC	5 cut(s)	55, 216, 312, 1321, 1802
AspS9I	GGNCC	5 cut(s)	96, 254, 264, 584, 1129
AsuC2I	CCSGG	2 cut(s)	259, 1466
AsuHPI	GGTGA	4 cut(s)	326, 551, 1292, 1874
AsuNHI	GCTAGC	1 cut(s)	1411
AvaII	GGWCC	4 cut(s)	254, 264, 584, 1129
AvrII	CCTAGG	1 cut(s)	647
BaeGI	GKGCMC	1 cut(s)	696
BamHI	GGATCC	1 cut(s)	1369
BanI	GGYRCC	1 cut(s)	1495
BanII	GRGCYC	1 cut(s)	123
BbsI	GAAGAC	1 cut(s)	1650
Bbv12I	GWGCWC	2 cut(s)	123, 1835
BbvCI	CCTCAGC	1 cut(s)	1626
BccI	CCATC	3 cut(s)	89, 710, 1271
BceAI	ACGGC	3 cut(s)	63, 167, 227
BciT130I	CCWGG	1 cut(s)	1367
BcnI	CCSGG	2 cut(s)	259, 1466
BcoDI	GTCTC	2 cut(s)	436, 1027
BfaI	CTAG	9 cut(s)	194, 648, 942, 1094, 1232, 1412, 1500, 1662, 1686
BfmI	CTRYAG	2 cut(s)	822, 1936
BfoI	RGCGCY	3 cut(s)	56, 313, 1803
BfrI	CTTAAG	2 cut(s)	1337, 1667
BfuAI	ACCTGC	3 cut(s)	275, 791, 798
BlnI	CCTAGG	1 cut(s)	647
BlpI	GCTNAGC	1 cut(s)	1539
Bme1390I	CCNGG	3 cut(s)	259, 1367, 1466
Bme18I	GGWCC	4 cut(s)	254, 264, 584, 1129
BmgT120I	GGNCC	5 cut(s)	96, 254, 264, 584, 1129
BmiI	GGNNCC	5 cut(s)	97, 256, 1131, 1371, 1497
BmrFI	CCNGG	3 cut(s)	259, 1367, 1466
BmsI	GCATC	4 cut(s)	151, 787, 1386, 1546
BmtI	GCTAGC	1 cut(s)	1415
BpiI	GAAGAC	1 cut(s)	1650
BplI	GAGNNNNNCTC	2 cut(s)	278, 310
Bpu10I	CCTNAGC	3 cut(s)	1491, 1620, 1626
Bpu1102I	GCTNAGC	1 cut(s)	1539
BpuEI	CTTGAG	3 cut(s)	418, 1515, 1788
BpuMI	CCSGG	2 cut(s)	259, 1466
BsaHI	GRCGYC	2 cut(s)	90, 1655
BsaI	GGTCTC	1 cut(s)	1027
BsaJI	CCNNGG	2 cut(s)	257, 647
Bsc4I	CCNNNNNNNGG	4 cut(s)	470, 1691, 1850, 1851
Bse118I	RCCGGY	1 cut(s)	701
Bse1I	ACTGG	2 cut(s)	541, 1899
BseBI	CCWGG	1 cut(s)	1367
BseDI	CCNNGG	2 cut(s)	257, 647
BseGI	GGATG	5 cut(s)	391, 404, 788, 1164, 1984
BseLI	CCNNNNNNNGG	4 cut(s)	470, 1691, 1850, 1851
BseMII	CTCAG	3 cut(s)	1617, 1634, 1731
BseNI	ACTGG	2 cut(s)	541, 1899
BseRI	GAGGAG	2 cut(s)	1727, 1786
BseSI	GKGCMC	1 cut(s)	696
BseYI	CCCAGC	1 cut(s)	34
BsgI	GTGCAG	1 cut(s)	1751
Bsh1285I	CGRYCG	1 cut(s)	9
BshFI	GGCC	5 cut(s)	98, 138, 284, 1365, 1457
BshNI	GGYRCC	1 cut(s)	1495
BsiEI	CGRYCG	1 cut(s)	9
BsiHKAI	GWGCWC	2 cut(s)	123, 1835
BsiSI	CCGG	3 cut(s)	259, 702, 1466
BslFI	GGGAC	2 cut(s)	983, 1407
BslI	CCNNNNNNNGG	4 cut(s)	470, 1691, 1850, 1851
BsmAI	GTCTC	2 cut(s)	436, 1027
BsmFI	GGGAC	2 cut(s)	983, 1407
BsmI	GAATGC	2 cut(s)	1321, 1489
BsnI	GGCC	5 cut(s)	98, 138, 284, 1365, 1457
Bso31I	GGTCTC	1 cut(s)	1027
Bsp1286I	GDGCHC	3 cut(s)	123, 696, 1835
Bsp143I	GATC	3 cut(s)	733, 1369, 1694
Bsp1720I	GCTNAGC	1 cut(s)	1539
BspACI	CCGC	6 cut(s)	27, 50, 180, 1133, 1766, 1851
BspANI	GGCC	5 cut(s)	98, 138, 284, 1365, 1457
BspCNI	CTCAG	3 cut(s)	1618, 1633, 1732
BspHI	TCATGA	1 cut(s)	640
BspLI	GGNNCC	5 cut(s)	97, 256, 1131, 1371, 1497
BspMAI	CTGCAG	2 cut(s)	826, 1940
BspMI	ACCTGC	3 cut(s)	275, 791, 798
BspOI	GCTAGC	1 cut(s)	1415
BspPI	GGATC	4 cut(s)	741, 1364, 1377, 1702
BspQI	GCTCTTC	1 cut(s)	1771
BspT107I	GGYRCC	1 cut(s)	1495
BspTI	CTTAAG	2 cut(s)	1337, 1667
BspTNI	GGTCTC	1 cut(s)	1027
BsrBI	CCGCTC	1 cut(s)	1851
BsrFI	RCCGGY	1 cut(s)	701
BsrI	ACTGG	2 cut(s)	541, 1899
BssAI	RCCGGY	1 cut(s)	701
BssECI	CCNNGG	2 cut(s)	257, 647
BssMI	GATC	3 cut(s)	733, 1369, 1694
BssNI	GRCGYC	2 cut(s)	90, 1655
BssT1I	CCWWGG	1 cut(s)	647
Bst2UI	CCWGG	1 cut(s)	1367
Bst4CI	ACNGT	7 cut(s)	786, 1027, 1199, 1274, 1449, 1837, 1964
Bst6I	CTCTTC	2 cut(s)	21, 1771
BstACI	GRCGYC	2 cut(s)	90, 1655
BstAFI	CTTAAG	2 cut(s)	1337, 1667
BstAPI	GCANNNNNTGC	3 cut(s)	276, 1763, 1947
BstC8I	GCNNGC	8 cut(s)	119, 314, 681, 1228, 1413, 1545, 1734, 1952
BstDEI	CTNAG	6 cut(s)	1491, 1539, 1620, 1626, 1740, 1796
BstF5I	GGATG	5 cut(s)	391, 404, 788, 1164, 1984
BstH2I	RGCGCY	3 cut(s)	56, 313, 1803
BstHHI	GCGC	5 cut(s)	55, 216, 312, 1321, 1802
BstKTI	GATC	3 cut(s)	736, 1372, 1697
BstMAI	GTCTC	2 cut(s)	436, 1027
BstMBI	GATC	3 cut(s)	733, 1369, 1694
BstMCI	CGRYCG	1 cut(s)	9
BstNI	CCWGG	1 cut(s)	1367
BstNSI	RCATGY	2 cut(s)	1411, 1954
BstSCI	CCNGG	3 cut(s)	257, 1365, 1464
BstSFI	CTRYAG	2 cut(s)	822, 1936
BstSLI	GKGCMC	1 cut(s)	696
BstV2I	GAAGAC	1 cut(s)	1650
BstX2I	RGATCY	1 cut(s)	1369
BstYI	RGATCY	1 cut(s)	1369
BsuRI	GGCC	5 cut(s)	98, 138, 284, 1365, 1457
BtsCI	GGATG	5 cut(s)	391, 404, 788, 1164, 1984
BtsI	GCAGTG	1 cut(s)	223
BtsIMutI	CAGTG	4 cut(s)	223, 1279, 1833, 1892
BveI	ACCTGC	3 cut(s)	275, 791, 798
Cac8I	GCNNGC	8 cut(s)	119, 314, 681, 1228, 1413, 1545, 1734, 1952
CaiI	CAGNNNCTG	2 cut(s)	1763, 1787
CciI	TCATGA	1 cut(s)	640
CfoI	GCGC	5 cut(s)	55, 216, 312, 1321, 1802
Cfr10I	RCCGGY	1 cut(s)	701
Cfr13I	GGNCC	5 cut(s)	96, 254, 264, 584, 1129
CseI	GACGC	1 cut(s)	827
Csp6I	GTAC	3 cut(s)	594, 1496, 1921
CviAII	CATG	6 cut(s)	641, 673, 1408, 1453, 1947, 1951
CviQI	GTAC	3 cut(s)	594, 1496, 1921
DdeI	CTNAG	6 cut(s)	1491, 1539, 1620, 1626, 1740, 1796
DpnI	GATC	3 cut(s)	735, 1371, 1696
DpnII	GATC	3 cut(s)	733, 1369, 1694
DraI	TTTAAA	1 cut(s)	418
DrdI	GACNNNNNNGTC	1 cut(s)	428
DseDI	GACNNNNNNGTC	1 cut(s)	428
Eam1104I	CTCTTC	2 cut(s)	21, 1771
EarI	CTCTTC	2 cut(s)	21, 1771
EciI	GGCGGA	1 cut(s)	169
Ecl136II	GAGCTC	1 cut(s)	121
Eco130I	CCWWGG	1 cut(s)	647
Eco147I	AGGCCT	1 cut(s)	1365
Eco24I	GRGCYC	1 cut(s)	123
Eco31I	GGTCTC	1 cut(s)	1027
Eco47I	GGWCC	4 cut(s)	254, 264, 584, 1129
Eco47III	AGCGCT	2 cut(s)	311, 1801
Eco53kI	GAGCTC	1 cut(s)	121
Eco57I	CTGAAG	4 cut(s)	609, 1128, 1242, 1809
EcoICRI	GAGCTC	1 cut(s)	121
EcoO109I	RGGNCCY	2 cut(s)	254, 1129
EcoRI	GAATTC	1 cut(s)	1253
EcoRII	CCWGG	1 cut(s)	1365
EcoT14I	CCWWGG	1 cut(s)	647
EcoT22I	ATGCAT	2 cut(s)	1952, 1956
EcoT38I	GRGCYC	1 cut(s)	123
ErhI	CCWWGG	1 cut(s)	647
FaeI	CATG	6 cut(s)	644, 676, 1411, 1456, 1950, 1954
FalI	AAGNNNNNCTT	4 cut(s)	867, 899, 887, 919
FaqI	GGGAC	2 cut(s)	983, 1407
FatI	CATG	6 cut(s)	640, 672, 1407, 1452, 1946, 1950
FauI	CCCGC	2 cut(s)	1140, 1844
FokI	GGATG	4 cut(s)	391, 398, 775, 1151
FriOI	GRGCYC	1 cut(s)	123
FspBI	CTAG	9 cut(s)	194, 648, 942, 1094, 1232, 1412, 1500, 1662, 1686
GlaI	GCGC	5 cut(s)	54, 215, 311, 1320, 1801
GsaI	CCCAGC	1 cut(s)	38
HaeII	RGCGCY	3 cut(s)	56, 313, 1803
HaeIII	GGCC	5 cut(s)	98, 138, 284, 1365, 1457
HapII	CCGG	3 cut(s)	259, 702, 1466
HgaI	GACGC	1 cut(s)	827
HhaI	GCGC	5 cut(s)	55, 216, 312, 1321, 1802
Hin1I	GRCGYC	2 cut(s)	90, 1655
Hin1II	CATG	6 cut(s)	644, 676, 1411, 1456, 1950, 1954
Hin6I	GCGC	5 cut(s)	53, 214, 310, 1319, 1800
HinP1I	GCGC	5 cut(s)	53, 214, 310, 1319, 1800
HincII	GTYRAC	2 cut(s)	402, 1299
HindII	GTYRAC	2 cut(s)	402, 1299
HindIII	AAGCTT	2 cut(s)	1224, 1647
HpaI	GTTAAC	1 cut(s)	402
HpaII	CCGG	3 cut(s)	259, 702, 1466
HphI	GGTGA	4 cut(s)	326, 551, 1292, 1874
Hpy166II	GTNNAC	4 cut(s)	221, 264, 402, 1299
Hpy188I	TCNGA	9 cut(s)	328, 409, 451, 534, 604, 996, 1032, 1204, 1433
Hpy188III	TCNNGA	7 cut(s)	582, 641, 875, 1211, 1326, 1692, 1973
Hpy8I	GTNNAC	4 cut(s)	221, 264, 402, 1299
Hpy99I	CGWCG	6 cut(s)	92, 95, 188, 248, 843, 1657
HpyAV	CCTTC	6 cut(s)	134, 373, 539, 584, 754, 948
HpyCH4III	ACNGT	7 cut(s)	786, 1027, 1199, 1274, 1449, 1837, 1964
HpyCH4IV	ACGT	4 cut(s)	90, 345, 632, 1655
HpyF3I	CTNAG	6 cut(s)	1491, 1539, 1620, 1626, 1740, 1796
HpySE526I	ACGT	4 cut(s)	90, 345, 632, 1655
Hsp92I	GRCGYC	2 cut(s)	90, 1655
Hsp92II	CATG	6 cut(s)	644, 676, 1411, 1456, 1950, 1954
HspAI	GCGC	5 cut(s)	53, 214, 310, 1319, 1800
KpnI	GGTACC	1 cut(s)	1499
KspAI	GTTAAC	1 cut(s)	402
Kzo9I	GATC	3 cut(s)	733, 1369, 1694
LguI	GCTCTTC	1 cut(s)	1771
LmnI	GCTCC	5 cut(s)	126, 827, 940, 1114, 1880
LweI	GCATC	4 cut(s)	151, 787, 1386, 1546
MaeI	CTAG	9 cut(s)	194, 648, 942, 1094, 1232, 1412, 1500, 1662, 1686
MaeII	ACGT	4 cut(s)	90, 345, 632, 1655
MaeIII	GTNAC	4 cut(s)	568, 719, 1268, 1862
MalI	GATC	3 cut(s)	735, 1371, 1696
MbiI	CCGCTC	1 cut(s)	1851
MboI	GATC	3 cut(s)	733, 1369, 1694
MboII	GAAGA	9 cut(s)	8, 76, 97, 123, 467, 903, 1600, 1650, 1788
MfeI	CAATTG	1 cut(s)	1070
MflI	RGATCY	1 cut(s)	1369
MhlI	GDGCHC	3 cut(s)	123, 696, 1835
MluCI	AATT	8 cut(s)	175, 870, 997, 1070, 1253, 1583, 1592, 1924
MlyI	GAGTC	3 cut(s)	104, 287, 1393
MmeI	TCCRAC	4 cut(s)	192, 351, 557, 1500
Mph1103I	ATGCAT	2 cut(s)	1952, 1956
MseI	TTAA	9 cut(s)	353, 401, 417, 665, 963, 1152, 1338, 1668, 1869
MslI	CAYNNNNRTG	1 cut(s)	753
MspA1I	CMGCKG	4 cut(s)	955, 1739, 1760, 1787
MspCI	CTTAAG	2 cut(s)	1337, 1667
MspI	CCGG	3 cut(s)	259, 702, 1466
MspR9I	CCNGG	3 cut(s)	259, 1367, 1466
MunI	CAATTG	1 cut(s)	1070
Mva1269I	GAATGC	2 cut(s)	1321, 1489
MvaI	CCWGG	1 cut(s)	1367
NciI	CCSGG	2 cut(s)	259, 1466
NdeII	GATC	3 cut(s)	733, 1369, 1694
NheI	GCTAGC	1 cut(s)	1411
NlaIII	CATG	6 cut(s)	644, 676, 1411, 1456, 1950, 1954
NlaIV	GGNNCC	5 cut(s)	97, 256, 1131, 1371, 1497
NmeAIII	GCCGAG	2 cut(s)	310, 1033
NmuCI	GTSAC	2 cut(s)	1268, 1862
NsiI	ATGCAT	2 cut(s)	1952, 1956
NspI	RCATGY	2 cut(s)	1411, 1954
PaeI	GCATGC	1 cut(s)	1954
PagI	TCATGA	1 cut(s)	640
PaqCI	CACCTGC	1 cut(s)	791
PceI	AGGCCT	1 cut(s)	1365
PciSI	GCTCTTC	1 cut(s)	1771
PcsI	WCGNNNNNNNCGW	2 cut(s)	96, 114
PctI	GAATGC	2 cut(s)	1321, 1489
PfeI	GAWTC	7 cut(s)	339, 410, 578, 669, 908, 1526, 1969
PleI	GAGTC	3 cut(s)	104, 287, 1393
PpsI	GAGTC	3 cut(s)	104, 287, 1393
PpuMI	RGGWCCY	2 cut(s)	254, 1129
PsiI	TTATAA	2 cut(s)	846, 1044
Psp124BI	GAGCTC	1 cut(s)	123
Psp1406I	AACGTT	1 cut(s)	632
Psp5II	RGGWCCY	2 cut(s)	254, 1129
Psp6I	CCWGG	1 cut(s)	1365
PspFI	CCCAGC	1 cut(s)	34
PspGI	CCWGG	1 cut(s)	1365
PspN4I	GGNNCC	5 cut(s)	97, 256, 1131, 1371, 1497
PspPI	GGNCC	5 cut(s)	96, 254, 264, 584, 1129
PspPPI	RGGWCCY	2 cut(s)	254, 1129
PstI	CTGCAG	2 cut(s)	826, 1940
PstNI	CAGNNNCTG	2 cut(s)	1763, 1787
PsuI	RGATCY	1 cut(s)	1369
PvuII	CAGCTG	4 cut(s)	955, 1739, 1760, 1787
RsaI	GTAC	3 cut(s)	595, 1497, 1922
RsaNI	GTAC	3 cut(s)	594, 1496, 1921
RseI	CAYNNNNRTG	1 cut(s)	753
SacI	GAGCTC	1 cut(s)	123
SapI	GCTCTTC	1 cut(s)	1771
SaqAI	TTAA	9 cut(s)	353, 401, 417, 665, 963, 1152, 1338, 1668, 1869
Sau3AI	GATC	3 cut(s)	733, 1369, 1694
Sau96I	GGNCC	5 cut(s)	96, 254, 264, 584, 1129
SchI	GAGTC	3 cut(s)	104, 287, 1393
ScrFI	CCNGG	3 cut(s)	259, 1367, 1466
SduI	GDGCHC	3 cut(s)	123, 696, 1835
SfaNI	GCATC	4 cut(s)	151, 787, 1386, 1546
SfcI	CTRYAG	2 cut(s)	822, 1936
SinI	GGWCC	4 cut(s)	254, 264, 584, 1129
SmiMI	CAYNNNNRTG	1 cut(s)	753
SmlI	CTYRAG	6 cut(s)	433, 1337, 1530, 1667, 1803, 1973
SmoI	CTYRAG	6 cut(s)	433, 1337, 1530, 1667, 1803, 1973
SphI	GCATGC	1 cut(s)	1954
Sse9I	AATT	8 cut(s)	175, 870, 997, 1070, 1253, 1583, 1592, 1924
SseBI	AGGCCT	1 cut(s)	1365
SsiI	CCGC	6 cut(s)	27, 50, 180, 1133, 1766, 1851
SspMI	CTAG	9 cut(s)	194, 648, 942, 1094, 1232, 1412, 1500, 1662, 1686
SstI	GAGCTC	1 cut(s)	123
StuI	AGGCCT	1 cut(s)	1365
StyD4I	CCNGG	3 cut(s)	257, 1365, 1464
StyI	CCWWGG	1 cut(s)	647
TaaI	ACNGT	7 cut(s)	786, 1027, 1199, 1274, 1449, 1837, 1964
TaiI	ACGT	4 cut(s)	93, 348, 635, 1658
TaqI	TCGA	8 cut(s)	87, 108, 186, 204, 291, 1185, 1210, 1652
TaqII	GACCGA	1 cut(s)	23
TasI	AATT	8 cut(s)	175, 870, 997, 1070, 1253, 1583, 1592, 1924
TauI	GCSGC	1 cut(s)	53
TfiI	GAWTC	7 cut(s)	339, 410, 578, 669, 908, 1526, 1969
Tru1I	TTAA	9 cut(s)	353, 401, 417, 665, 963, 1152, 1338, 1668, 1869
Tru9I	TTAA	9 cut(s)	353, 401, 417, 665, 963, 1152, 1338, 1668, 1869
TscAI	CASTG	4 cut(s)	223, 1279, 1840, 1899
TseFI	GTSAC	2 cut(s)	1268, 1862
Tsp45I	GTSAC	2 cut(s)	1268, 1862
TspRI	CASTG	4 cut(s)	223, 1279, 1840, 1899
Vha464I	CTTAAG	2 cut(s)	1337, 1667
VpaK11BI	GGWCC	4 cut(s)	254, 264, 584, 1129
XapI	RAATTY	2 cut(s)	870, 1253
XceI	RCATGY	2 cut(s)	1411, 1954
XcmI	CCANNNNNNNNNTGG	1 cut(s)	533
XmaJI	CCTAGG	1 cut(s)	647
XspI	CTAG	9 cut(s)	194, 648, 942, 1094, 1232, 1412, 1500, 1662, 1686
ZraI	GACGTC	2 cut(s)	91, 1656
Zsp2I	ATGCAT	2 cut(s)	1952, 1956

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.