Rh1BG199100

Ankyrin repeat and zinc finger domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0007214
Physical Location & Seq
Reverse (-)
31564101 .. 31568541
4441 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG199100.1

Sequence Viewer

Length: 1689 bp
ATGGCGTCGGAGCCTCCTCGCACCACCACGTCATCCACCACGACCGCCGCCGAAAAAGAGAAACGACACCGTTCCATCTTCGAACTCCCGGCCAACTTCTTCGACTCCTGCGCCCTCCTCACTTCTTCCGGCCTCTCAGTTGCTTCAACTTTCGAGACCTCTAACAACAGCAACAACTCCGCCCTCGAAACCCTAGACGAGTCTGTGTCCGATAACACCGATCAAGACCGCTCCCTAAACGCCGCCGTATCGACTCGGTGGACCTGCAACACTTGCAAGGCTGAGTTCGAGTCCCTCCAAGAGCAGCAATTTCACTTCAAATCCGATATTCACCGATTCAATGTGAAGCTAAGCCTTGCTGGAAAGAATATTGTGAAGGAGGAGGACTTTGATGAGTTAACGTCTGATTCTTTTAAAGACTTTGATGTCTCGAGTATATCGGGATCAGAGGATGATGAACCTGAAAAAGTTGGCCCTTTCAAGCTTTCAAGGGCAACTGCTAGGCAGAAATTACTCATCCACCTTCAGACGGGAGAAAGAATTTCGATTTGGAAGTGTTTGATTACAAATGAATCTGGACCTGAAGGTAATTTGTCGTGTGATGAATTGTTGGGGAGATTGAACACGGTGGTTCATGAGCCTAGAGATAATACCTGTTTTAGAGTTGTGTTGCTTGCAAGTGGTGGGCACTTTGCTGGTTGTGTGTTTGATGGTAGCAAGATTGTAGCTCACAAGACTTTCCACAGGTATGTCGTAAGGGCCAAGTCTGGAAAAAAACAGTCATCCAATGATGCATCTGGAAGGTTTGCTCATTCCGCTGGAGCTTCTCTTCGTCGCCATAATGAACTTGCATTGAAAAAGGATATTCAAGAGCTACTTGCTGCATGGAAGCCTTATTTTGATGCTTCGTCTTGCGTTTTTGTTTATGCTCCTTCTAGTAATCATCAGCTGCTATCTAATAGGGAGACACCATATTTTAGTCATCAGAAAGTCCGAAATATTCCAATCCCTATTCGGAGACCTACTATGAAGGAAGCTCGGCGGATATATGACCAGTTGACACAAGTCTCTTATGAACAAGAGGAGAATGAAACTCCTTTAGTTACCAAAAAGGAATTGCCGTCTAGTTCAGGAATTGAAAATGATATCATCTCAAGCATGGTGAAAGTCAACATTGTTGAGCCTTTTGTTGAGCCTAGTACAGAAGCTTGCTCAAGTATTAGAGAGTCCAATGAACTCCCTATATCAAGTGAGAGTAGTAATGAGGTTAAAGGTTCGTCAACACCTTTGCATGAGGCAGCAGAGTCTGGGAATGCTCTCAAGGTTTTGGAACTCTTGGAACAGGGTCTGGATCCTTGTGCCAGAGATGAAAGGGGACAGACTCCATACACGGTAGCAAACGAAAAGGAGGTCAGAAACACATTTAGACGGTTCATGGCCTCAAATCTAGATAAGTGGGACTGGAATGCTGCTAAGGTACCTAGTGCATTGACAAAGGAGATGGAGGAATCTCAAGCTGCTAAGCAGGAGGAACTGAAAAGGGCACTGGTAGCCGAGAGGGAAAAACGAGCAGCCGCAGCCGAGCGGAGAATGGCTGCAGCTGCAGCCCTTAGTGCTCAAGTAATTGAAGCAAATGGCAATAGTAGGAATATTTGTGACAATGAGAATGTAGATGTGCTCCGACTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

562

Amino Acids

62.45

Weight (kDa)

5.7

Isoelectric Point (pI)

50.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bVLRF1 PF18826 222 - 360 6.6e-47 Bacteroidetes VLRF1 release factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 425
Acc36I ACCTGC 1 cut(s) 272
Acc65I GGTACC 1 cut(s) 1477
AccB1I GGYRCC 1 cut(s) 1477
AccBSI CCGCTC 2 cut(s) 231, 1585
AciI CCGC 9 cut(s) 45, 48, 180, 229, 243, 816, 1042, 1575, 1585
AclWI GGATC 3 cut(s) 451, 1346, 1359
AcoI YGGCCR 1 cut(s) 90
AcsI RAATTY 1 cut(s) 540
AcuI CTGAAG 2 cut(s) 509, 603
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 1201, 1479
AfiI CCNNNNNNNGG 1 cut(s) 529
AjiI CACGTC 1 cut(s) 30
AjuI GAANNNNNNNTTGG 2 cut(s) 532, 564
Alw21I GWGCWC 2 cut(s) 1618, 1680
Alw26I GTCTC 5 cut(s) 149, 433, 959, 1012, 1072
AlwI GGATC 3 cut(s) 451, 1346, 1359
AlwNI CAGNNNCTG 2 cut(s) 1307, 1348
Ama87I CYCGRG 1 cut(s) 430
AoxI GGCC 5 cut(s) 90, 130, 472, 759, 1437
ApoI RAATTY 1 cut(s) 540
ArsI GACNNNNNNTTYG 2 cut(s) 1395, 1427
Asp718I GGTACC 1 cut(s) 1477
AspLEI GCGC 1 cut(s) 113
AspS9I GGNCC 4 cut(s) 261, 473, 578, 759
AsuC2I CCSGG 1 cut(s) 89
AsuHPI GGTGA 2 cut(s) 323, 1174
AsuII TTCGAA 1 cut(s) 81
AvaI CYCGRG 1 cut(s) 430
AvaII GGWCC 2 cut(s) 261, 578
BaeGI GKGCMC 2 cut(s) 690, 1546
BamHI GGATCC 1 cut(s) 1351
BanI GGYRCC 1 cut(s) 1477
Bbv12I GWGCWC 2 cut(s) 1618, 1680
BccI CCATC 3 cut(s) 83, 704, 1495
BceAI ACGGC 2 cut(s) 230, 1105
BcnI CCSGG 1 cut(s) 89
BcoDI GTCTC 5 cut(s) 149, 433, 959, 1012, 1072
BfaI CTAG 8 cut(s) 194, 501, 642, 936, 1125, 1197, 1448, 1482
BfmI CTRYAG 3 cut(s) 1596, 1602, 1685
BfuAI ACCTGC 1 cut(s) 272
BlpI GCTNAGC 2 cut(s) 350, 1521
Bme1390I CCNGG 1 cut(s) 89
Bme18I GGWCC 2 cut(s) 261, 578
BmeT110I CYCGRG 1 cut(s) 430
BmgBI CACGTC 1 cut(s) 30
BmgT120I GGNCC 4 cut(s) 261, 473, 578, 759
BmiI GGNNCC 3 cut(s) 12, 1353, 1479
BmrFI CCNGG 1 cut(s) 89
BmsI GCATC 3 cut(s) 781, 803, 892
BoxI GACNNNNGTC 1 cut(s) 1064
BpmI CTGGAG 1 cut(s) 840
Bpu10I CCTNAGC 1 cut(s) 1473
Bpu1102I GCTNAGC 2 cut(s) 350, 1521
Bpu14I TTCGAA 1 cut(s) 81
BpuEI CTTGAG 5 cut(s) 1138, 1198, 1304, 1497, 1602
BpuMI CCSGG 1 cut(s) 89
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 2 cut(s) 149, 1012
Bsc4I CCNNNNNNNGG 1 cut(s) 529
Bse1I ACTGG 3 cut(s) 1054, 1466, 1551
BseGI GGATG 4 cut(s) 32, 457, 516, 782
BseLI CCNNNNNNNGG 1 cut(s) 529
BseMII CTCAG 2 cut(s) 150, 273
BseNI ACTGG 3 cut(s) 1054, 1466, 1551
BseRI GAGGAG 4 cut(s) 6, 107, 395, 1097
BseSI GKGCMC 2 cut(s) 690, 1546
Bsh1285I CGRYCG 1 cut(s) 45
BshFI GGCC 5 cut(s) 92, 132, 474, 761, 1439
BshNI GGYRCC 1 cut(s) 1477
BsiEI CGRYCG 1 cut(s) 45
BsiHKAI GWGCWC 2 cut(s) 1618, 1680
BsiHKCI CYCGRG 1 cut(s) 430
BsiSI CCGG 2 cut(s) 89, 129
BslFI GGGAC 3 cut(s) 277, 1389, 1472
BslI CCNNNNNNNGG 1 cut(s) 529
BsmAI GTCTC 5 cut(s) 149, 433, 959, 1012, 1072
BsmFI GGGAC 3 cut(s) 277, 1389, 1472
BsmI GAATGC 2 cut(s) 1318, 1471
BsnI GGCC 5 cut(s) 92, 132, 474, 761, 1439
Bso31I GGTCTC 2 cut(s) 149, 1012
BsoBI CYCGRG 1 cut(s) 430
Bsp119I TTCGAA 1 cut(s) 81
Bsp1286I GDGCHC 4 cut(s) 690, 1546, 1618, 1680
Bsp143I GATC 3 cut(s) 220, 443, 1351
Bsp1720I GCTNAGC 2 cut(s) 350, 1521
BspACI CCGC 9 cut(s) 45, 48, 180, 229, 243, 816, 1042, 1575, 1585
BspANI GGCC 5 cut(s) 92, 132, 474, 761, 1439
BspCNI CTCAG 2 cut(s) 149, 274
BspHI TCATGA 1 cut(s) 634
BspLI GGNNCC 3 cut(s) 12, 1353, 1479
BspMAI CTGCAG 2 cut(s) 1600, 1606
BspMI ACCTGC 1 cut(s) 272
BspPI GGATC 3 cut(s) 451, 1346, 1359
BspT104I TTCGAA 1 cut(s) 81
BspT107I GGYRCC 1 cut(s) 1477
BspTNI GGTCTC 2 cut(s) 149, 1012
BsrBI CCGCTC 2 cut(s) 231, 1585
BsrI ACTGG 3 cut(s) 1054, 1466, 1551
BssMI GATC 3 cut(s) 220, 443, 1351
BssNI GRCGYC 1 cut(s) 5
Bst4CI ACNGT 6 cut(s) 71, 628, 780, 1393, 1431, 1686
Bst6I CTCTTC 1 cut(s) 834
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 273
BstBI TTCGAA 1 cut(s) 81
BstC8I GCNNGC 2 cut(s) 675, 1210
BstDEI CTNAG 6 cut(s) 136, 282, 350, 1473, 1521, 1610
BstF5I GGATG 4 cut(s) 32, 457, 516, 782
BstHHI GCGC 1 cut(s) 113
BstKTI GATC 3 cut(s) 223, 446, 1354
BstMAI GTCTC 5 cut(s) 149, 433, 959, 1012, 1072
BstMBI GATC 3 cut(s) 220, 443, 1351
BstMCI CGRYCG 1 cut(s) 45
BstMWI GCNNNNNNNGC 7 cut(s) 273, 815, 1550, 1577, 1601, 1604, 1613
BstPAI GACNNNNGTC 1 cut(s) 1064
BstSCI CCNGG 1 cut(s) 87
BstSFI CTRYAG 3 cut(s) 1596, 1602, 1685
BstSLI GKGCMC 2 cut(s) 690, 1546
BstX2I RGATCY 1 cut(s) 1351
BstYI RGATCY 1 cut(s) 1351
BsuRI GGCC 5 cut(s) 92, 132, 474, 761, 1439
BtrI CACGTC 1 cut(s) 30
BtsCI GGATG 4 cut(s) 32, 457, 516, 782
BtsIMutI CAGTG 1 cut(s) 1544
BveI ACCTGC 1 cut(s) 272
Cac8I GCNNGC 2 cut(s) 675, 1210
CaiI CAGNNNCTG 2 cut(s) 1307, 1348
CciI TCATGA 1 cut(s) 634
CfoI GCGC 1 cut(s) 113
Cfr13I GGNCC 4 cut(s) 261, 473, 578, 759
Csp6I GTAC 2 cut(s) 1200, 1478
CviAII CATG 5 cut(s) 635, 885, 1159, 1292, 1435
CviQI GTAC 2 cut(s) 1200, 1478
DdeI CTNAG 6 cut(s) 136, 282, 350, 1473, 1521, 1610
DpnI GATC 3 cut(s) 222, 445, 1353
DpnII GATC 3 cut(s) 220, 443, 1351
DraI TTTAAA 1 cut(s) 415
DrdI GACNNNNNNGTC 1 cut(s) 425
DseDI GACNNNNNNGTC 1 cut(s) 425
EaeI YGGCCR 1 cut(s) 90
Eam1104I CTCTTC 1 cut(s) 834
EarI CTCTTC 1 cut(s) 834
EciI GGCGGA 2 cut(s) 169, 1057
Eco31I GGTCTC 2 cut(s) 149, 1012
Eco32I GATATC 1 cut(s) 1147
Eco47I GGWCC 2 cut(s) 261, 578
Eco57I CTGAAG 2 cut(s) 509, 603
Eco88I CYCGRG 1 cut(s) 430
EcoRV GATATC 1 cut(s) 1147
EcoT22I ATGCAT 1 cut(s) 796
FaeI CATG 5 cut(s) 638, 888, 1162, 1295, 1438
FalI AAGNNNNNCTT 2 cut(s) 861, 893
FaqI GGGAC 3 cut(s) 277, 1389, 1472
FatI CATG 5 cut(s) 634, 884, 1158, 1291, 1434
FokI GGATG 4 cut(s) 19, 464, 503, 769
FspBI CTAG 8 cut(s) 194, 501, 642, 936, 1125, 1197, 1448, 1482
GlaI GCGC 1 cut(s) 112
GsuI CTGGAG 1 cut(s) 840
HaeIII GGCC 5 cut(s) 92, 132, 474, 761, 1439
HapII CCGG 2 cut(s) 89, 129
HhaI GCGC 1 cut(s) 113
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 5 cut(s) 638, 888, 1162, 1295, 1438
Hin6I GCGC 1 cut(s) 111
HinP1I GCGC 1 cut(s) 111
HincII GTYRAC 4 cut(s) 399, 1059, 1171, 1281
HindII GTYRAC 4 cut(s) 399, 1059, 1171, 1281
HindIII AAGCTT 2 cut(s) 482, 1206
HpaI GTTAAC 1 cut(s) 399
HpaII CCGG 2 cut(s) 89, 129
HphI GGTGA 2 cut(s) 323, 1174
Hpy166II GTNNAC 5 cut(s) 261, 399, 1059, 1171, 1281
Hpy8I GTNNAC 5 cut(s) 261, 399, 1059, 1171, 1281
Hpy99I CGWCG 2 cut(s) 10, 837
HpyAV CCTTC 6 cut(s) 370, 533, 578, 795, 942, 1024
HpyCH4III ACNGT 6 cut(s) 71, 628, 780, 1393, 1431, 1686
HpyCH4IV ACGT 2 cut(s) 29, 401
HpyF10VI GCNNNNNNNGC 7 cut(s) 273, 815, 1550, 1577, 1601, 1604, 1613
HpyF3I CTNAG 6 cut(s) 136, 282, 350, 1473, 1521, 1610
HpySE526I ACGT 2 cut(s) 29, 401
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 5 cut(s) 638, 888, 1162, 1295, 1438
HspAI GCGC 1 cut(s) 111
KpnI GGTACC 1 cut(s) 1481
KspAI GTTAAC 1 cut(s) 399
Kzo9I GATC 3 cut(s) 220, 443, 1351
LmnI GCTCC 5 cut(s) 10, 236, 821, 934, 1683
LweI GCATC 3 cut(s) 781, 803, 892
MaeI CTAG 8 cut(s) 194, 501, 642, 936, 1125, 1197, 1448, 1482
MaeII ACGT 2 cut(s) 29, 401
MaeIII GTNAC 2 cut(s) 1102, 1655
MalI GATC 3 cut(s) 222, 445, 1353
MbiI CCGCTC 2 cut(s) 231, 1585
MboI GATC 3 cut(s) 220, 443, 1351
MboII GAAGA 4 cut(s) 70, 91, 117, 821
MflI RGATCY 1 cut(s) 1351
MhlI GDGCHC 4 cut(s) 690, 1546, 1618, 1680
MluCI AATT 8 cut(s) 308, 509, 540, 589, 605, 1115, 1134, 1623
MlyI GAGTC 7 cut(s) 98, 209, 247, 299, 1235, 1313, 1375
Mph1103I ATGCAT 1 cut(s) 796
MseI TTAA 3 cut(s) 398, 414, 1269
MslI CAYNNNNRTG 1 cut(s) 747
MspA1I CMGCKG 3 cut(s) 818, 949, 1601
MspI CCGG 2 cut(s) 89, 129
MspR9I CCNGG 1 cut(s) 89
Mva1269I GAATGC 2 cut(s) 1318, 1471
MwoI GCNNNNNNNGC 7 cut(s) 273, 815, 1550, 1577, 1601, 1604, 1613
NciI CCSGG 1 cut(s) 89
NdeII GATC 3 cut(s) 220, 443, 1351
NlaIII CATG 5 cut(s) 638, 888, 1162, 1295, 1438
NlaIV GGNNCC 3 cut(s) 12, 1353, 1479
NmeAIII GCCGAG 3 cut(s) 1018, 1579, 1606
NmuCI GTSAC 1 cut(s) 1655
NsiI ATGCAT 1 cut(s) 796
NspV TTCGAA 1 cut(s) 81
PaeR7I CTCGAG 1 cut(s) 430
PagI TCATGA 1 cut(s) 634
PctI GAATGC 2 cut(s) 1318, 1471
PfeI GAWTC 4 cut(s) 336, 407, 572, 1508
PleI GAGTC 7 cut(s) 98, 208, 247, 298, 1234, 1312, 1375
PpsI GAGTC 7 cut(s) 98, 208, 247, 298, 1234, 1312, 1375
PshAI GACNNNNGTC 1 cut(s) 1064
PspN4I GGNNCC 3 cut(s) 12, 1353, 1479
PspPI GGNCC 4 cut(s) 261, 473, 578, 759
PstI CTGCAG 2 cut(s) 1600, 1606
PstNI CAGNNNCTG 2 cut(s) 1307, 1348
PsuI RGATCY 1 cut(s) 1351
PvuII CAGCTG 2 cut(s) 949, 1601
RsaI GTAC 2 cut(s) 1201, 1479
RsaNI GTAC 2 cut(s) 1200, 1478
RseI CAYNNNNRTG 1 cut(s) 747
SaqAI TTAA 3 cut(s) 398, 414, 1269
Sau3AI GATC 3 cut(s) 220, 443, 1351
Sau96I GGNCC 4 cut(s) 261, 473, 578, 759
SchI GAGTC 7 cut(s) 98, 209, 247, 299, 1235, 1313, 1375
ScrFI CCNGG 1 cut(s) 89
SduI GDGCHC 4 cut(s) 690, 1546, 1618, 1680
SfaNI GCATC 3 cut(s) 781, 803, 892
SfcI CTRYAG 3 cut(s) 1596, 1602, 1685
Sfr274I CTCGAG 1 cut(s) 430
SfuI TTCGAA 1 cut(s) 81
SinI GGWCC 2 cut(s) 261, 578
SlaI CTCGAG 1 cut(s) 430
SmiMI CAYNNNNRTG 1 cut(s) 747
SmlI CTYRAG 6 cut(s) 430, 1153, 1213, 1319, 1512, 1617
SmoI CTYRAG 6 cut(s) 430, 1153, 1213, 1319, 1512, 1617
Sse9I AATT 8 cut(s) 308, 509, 540, 589, 605, 1115, 1134, 1623
SsiI CCGC 9 cut(s) 45, 48, 180, 229, 243, 816, 1042, 1575, 1585
SspI AATATT 3 cut(s) 370, 1000, 1651
SspMI CTAG 8 cut(s) 194, 501, 642, 936, 1125, 1197, 1448, 1482
StyD4I CCNGG 1 cut(s) 87
TaaI ACNGT 6 cut(s) 71, 628, 780, 1393, 1431, 1686
TaiI ACGT 2 cut(s) 32, 404
TaqI TCGA 8 cut(s) 81, 102, 153, 186, 251, 288, 431, 545
TasI AATT 8 cut(s) 308, 509, 540, 589, 605, 1115, 1134, 1623
TatI WGTACW 1 cut(s) 1199
TauI GCSGC 3 cut(s) 50, 245, 1577
TfiI GAWTC 4 cut(s) 336, 407, 572, 1508
Tru1I TTAA 3 cut(s) 398, 414, 1269
Tru9I TTAA 3 cut(s) 398, 414, 1269
TscAI CASTG 1 cut(s) 1551
TseFI GTSAC 1 cut(s) 1655
Tsp45I GTSAC 1 cut(s) 1655
TspRI CASTG 1 cut(s) 1551
VpaK11BI GGWCC 2 cut(s) 261, 578
XapI RAATTY 1 cut(s) 540
XbaI TCTAGA 1 cut(s) 1447
XhoI CTCGAG 1 cut(s) 430
XspI CTAG 8 cut(s) 194, 501, 642, 936, 1125, 1197, 1448, 1482
Zsp2I ATGCAT 1 cut(s) 796
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.