RLG00000028448

Ankyrin repeat and zinc finger domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0007214
Physical Location & Seq
Forward (+)
22953309 .. 22957950
4642 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028448

Sequence Viewer

Length: 1878 bp
ATGGCGTCGGAGCCTCCTCGCACCACCACCACCACCACGTCATCCACCGCGACCGCCGCCGCAAAAGAGAAACGACACCGTTCCATCTTCGAACTCCCGGCCAACTTCTTCGACTCCTGCGCCCTCCTCACTTCTTCTGGCCTCTCAGTTGCTTCGACTTTCGAGACCTCCAACAACAGCAACTCCGCTCACGAAACCCTAGACGAGTCCGTGTCCGATAACACCGATCAAGACCGCTCCCTAAACGCCGCCGTATCGACTCGGTGGACCTGCAACACTTGCAAGGCTGAGTTCGAGTCCCTCCATGAGCAGCGATTTCACTTCAAATCCGACATTCACCGATTCAATGTGAAGCTAAGCCTTGCTGGAAAGAATATTGTGAAGGAGGAGGACTTTGATGAGTTCACGTCTGATTCTTTTAAAGACTTTGATGTCTCGAGTATATCGGGATCAGAGGATGATGAACCTGAAAAAGTTGGCCCTTTCAAGCTTTCAAGGGCAGCTGCTAGGCAGAAATTACTCATCCACCTTCAGACGGGAGAAAGAATTTCGATTTGGAAGTGTTTGATTACAAATGAATCTGGACCTGAAGGTAATTTGTCATGTGATGAATTGTTGGGGAGATTGAACACGGTGGTTCATGAGCCTAGAGATAATACCTGTTTTAGAGTTGTGTTGCTTGCAAGTGGTGGGCACTTTGCTGGTTGTGTGTTTGATGGTAGCAAGATTGTAGCTTACAAGACTTTCCACAGGTATGTCGTAAGGGCCAAGTCTGGAAAAAAACAGTCATCCAATGATGCATCTGGAAGGTTTGCTCATTCCGCTGGAGCTTCTCTTCGTCGCCATAATGAACTTGCATTGAAAAAGGATATTCAAGAGCTACTTGCTGCATGGAAGCCTTATTTTGTTGCTTCGTCTTGCGTTTTTGTTTATGCTCCTTCTAGTAATTATCAGCTGCTATCAAATAGGGAGACACCATATTTTAGTCATCAGAAAGTCCGAAATATTCCAATCTCTATTCGGAGACCTACTATGAAGGAAGCTCGGCGGATATATGACCAGTTGACACAAGTCTCTTATGAACAAGAGGAGAATGAAGCTTCTTTAGTTACCAAAAAGGAATTGCCGTCTAGTTCAGGAATTGAAAATGATATCATCTCAAGCATGGCGAAAGTCAACATTGTTGAGCCTTTTGTTGAGCCTAGTACAGAAGCTTGCTCAAGTACTAGAGAGTCCAATGAACTCCCTATATCAAGTGAGAGTAGTAATGAGGTTAAAGGTTCATCAACACCTTTGCATGAGGCAGCACAGTCTGGGAATGCTCTCAAGGTTTTGGAACTCTTGGAACAGGGTCTGGATCCTTGTGCCAGAGATGAAAGGGGACAGACTCCATACACGGTAGCAAATGAAAAGGAGGTCAGAAACACATTTAGACGGTTCATGGCCTCAAATCTAGATAAGTGGGACTGGAATGCTGCTAAGGTACCTAGTGCATTGACAAAGGAGCTGGAAGAATCTCAAGCTGCTAAGCAGGCTGAAAAAGAAGCGAAAAGGAAAGCTAAAGCAAAAGAATTGAAGAAACTACGCAAAGAAAAAGAAAAGAAAGCTCAGGCTGAGGCTGCTGCTTCCAAGAATACTTCAACCATTACAAAGTCCTCTTCATCATCTCAGCCCAGTCGTGCATCACAGATCTCTAAAGAGGAGGAACTAAAAAGGGCACTGGTAGCCGAGAGGGAAAAACGAGCAGCCGCAGCTGAGCGGAGAATGGCTGCAGCTGCAGCCCTTAGTCCTCAAGGTAATAGCACCACTCCACAACCCAAAACTGGGGAAGCTGGAAGTTTTGAGATTCTGAAAGAATTTCACAACGATGGACTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

626

Amino Acids

69.08

Weight (kDa)

7.93

Isoelectric Point (pI)

49.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bVLRF1 PF18826 224 - 362 3.2e-44 Bacteroidetes VLRF1 release factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 431
Acc36I ACCTGC 1 cut(s) 278
Acc65I GGTACC 1 cut(s) 1483
AccB1I GGYRCC 1 cut(s) 1483
AccBSI CCGCTC 3 cut(s) 188, 237, 1759
AccII CGCG 1 cut(s) 50
AclWI GGATC 3 cut(s) 457, 1352, 1365
AcoI YGGCCR 1 cut(s) 99
AcsI RAATTY 2 cut(s) 546, 1856
AcuI CTGAAG 2 cut(s) 515, 609
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 3 cut(s) 1207, 1225, 1485
AfiI CCNNNNNNNGG 3 cut(s) 535, 1823, 1824
AjiI CACGTC 2 cut(s) 39, 408
AjuI GAANNNNNNNTTGG 2 cut(s) 538, 570
Alw26I GTCTC 5 cut(s) 158, 439, 965, 1018, 1078
AlwI GGATC 3 cut(s) 457, 1352, 1365
AlwNI CAGNNNCTG 1 cut(s) 1354
Ama87I CYCGRG 1 cut(s) 436
AoxI GGCC 5 cut(s) 99, 139, 478, 765, 1443
ApoI RAATTY 2 cut(s) 546, 1856
Asp718I GGTACC 1 cut(s) 1483
AspLEI GCGC 1 cut(s) 122
AspS9I GGNCC 4 cut(s) 267, 479, 584, 765
AsuC2I CCSGG 1 cut(s) 98
AsuHPI GGTGA 1 cut(s) 329
AsuII TTCGAA 1 cut(s) 90
AvaI CYCGRG 1 cut(s) 436
AvaII GGWCC 2 cut(s) 267, 584
BaeGI GKGCMC 2 cut(s) 696, 1720
BamHI GGATCC 1 cut(s) 1357
BanI GGYRCC 1 cut(s) 1483
BbvCI CCTCAGC 1 cut(s) 1614
BccI CCATC 3 cut(s) 92, 710, 1862
BceAI ACGGC 2 cut(s) 236, 1111
BcnI CCSGG 1 cut(s) 98
BcoDI GTCTC 5 cut(s) 158, 439, 965, 1018, 1078
BfaI CTAG 9 cut(s) 200, 507, 648, 942, 1131, 1203, 1227, 1454, 1488
BfmI CTRYAG 2 cut(s) 1770, 1776
BfuAI ACCTGC 1 cut(s) 278
BglII AGATCT 1 cut(s) 1689
BlpI GCTNAGC 3 cut(s) 356, 1527, 1755
BmcAI AGTACT 1 cut(s) 1225
Bme1390I CCNGG 1 cut(s) 98
Bme18I GGWCC 2 cut(s) 267, 584
BmeT110I CYCGRG 1 cut(s) 436
BmgBI CACGTC 2 cut(s) 39, 408
BmgT120I GGNCC 4 cut(s) 267, 479, 584, 765
BmiI GGNNCC 3 cut(s) 12, 1359, 1485
BmrFI CCNGG 1 cut(s) 98
BmrI ACTGGG 2 cut(s) 1668, 1833
BmsI GCATC 3 cut(s) 787, 809, 1691
BmuI ACTGGG 2 cut(s) 1668, 1833
BoxI GACNNNNGTC 1 cut(s) 1070
BpmI CTGGAG 1 cut(s) 846
Bpu10I CCTNAGC 3 cut(s) 1479, 1608, 1614
Bpu1102I GCTNAGC 3 cut(s) 356, 1527, 1755
Bpu14I TTCGAA 1 cut(s) 90
BpuEI CTTGAG 5 cut(s) 1144, 1204, 1310, 1503, 1776
BpuMI CCSGG 1 cut(s) 98
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 2 cut(s) 158, 1018
BsaXI ACNNNNNCTCC 2 cut(s) 167, 197
Bsc4I CCNNNNNNNGG 3 cut(s) 535, 1823, 1824
Bse1I ACTGG 5 cut(s) 1060, 1472, 1674, 1725, 1828
BseGI GGATG 4 cut(s) 41, 463, 522, 788
BseLI CCNNNNNNNGG 3 cut(s) 535, 1823, 1824
BseMII CTCAG 6 cut(s) 159, 279, 1605, 1622, 1682, 1746
BseNI ACTGG 5 cut(s) 1060, 1472, 1674, 1725, 1828
BseRI GAGGAG 5 cut(s) 6, 116, 401, 1103, 1715
BseSI GKGCMC 2 cut(s) 696, 1720
Bsh1236I CGCG 1 cut(s) 50
Bsh1285I CGRYCG 1 cut(s) 54
BshFI GGCC 5 cut(s) 101, 141, 480, 767, 1445
BshNI GGYRCC 1 cut(s) 1483
BsiEI CGRYCG 1 cut(s) 54
BsiHKCI CYCGRG 1 cut(s) 436
BsiSI CCGG 1 cut(s) 98
BslFI GGGAC 3 cut(s) 283, 1395, 1478
BslI CCNNNNNNNGG 3 cut(s) 535, 1823, 1824
BsmAI GTCTC 5 cut(s) 158, 439, 965, 1018, 1078
BsmFI GGGAC 3 cut(s) 283, 1395, 1478
BsmI GAATGC 2 cut(s) 1324, 1477
BsnI GGCC 5 cut(s) 101, 141, 480, 767, 1445
Bso31I GGTCTC 2 cut(s) 158, 1018
BsoBI CYCGRG 1 cut(s) 436
Bsp119I TTCGAA 1 cut(s) 90
Bsp1286I GDGCHC 2 cut(s) 696, 1720
Bsp143I GATC 4 cut(s) 226, 449, 1357, 1689
Bsp1720I GCTNAGC 3 cut(s) 356, 1527, 1755
BspANI GGCC 5 cut(s) 101, 141, 480, 767, 1445
BspCNI CTCAG 6 cut(s) 158, 280, 1606, 1621, 1681, 1747
BspFNI CGCG 1 cut(s) 50
BspHI TCATGA 1 cut(s) 640
BspLI GGNNCC 3 cut(s) 12, 1359, 1485
BspMAI CTGCAG 2 cut(s) 1774, 1780
BspMI ACCTGC 1 cut(s) 278
BspPI GGATC 3 cut(s) 457, 1352, 1365
BspT104I TTCGAA 1 cut(s) 90
BspT107I GGYRCC 1 cut(s) 1483
BspTNI GGTCTC 2 cut(s) 158, 1018
BsrBI CCGCTC 3 cut(s) 188, 237, 1759
BsrI ACTGG 5 cut(s) 1060, 1472, 1674, 1725, 1828
BssMI GATC 4 cut(s) 226, 449, 1357, 1689
BssNI GRCGYC 1 cut(s) 5
Bst4CI ACNGT 6 cut(s) 80, 634, 786, 1311, 1399, 1437
Bst6I CTCTTC 2 cut(s) 840, 1663
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 279
BstBI TTCGAA 1 cut(s) 90
BstC8I GCNNGC 3 cut(s) 681, 1216, 1533
BstF5I GGATG 4 cut(s) 41, 463, 522, 788
BstFNI CGCG 1 cut(s) 50
BstHHI GCGC 1 cut(s) 122
BstKTI GATC 4 cut(s) 229, 452, 1360, 1692
BstMAI GTCTC 5 cut(s) 158, 439, 965, 1018, 1078
BstMBI GATC 4 cut(s) 226, 449, 1357, 1689
BstMCI CGRYCG 1 cut(s) 54
BstMWI GCNNNNNNNGC 9 cut(s) 56, 279, 821, 1532, 1619, 1724, 1751, 1775, 1778
BstPAI GACNNNNGTC 1 cut(s) 1070
BstSCI CCNGG 1 cut(s) 96
BstSFI CTRYAG 2 cut(s) 1770, 1776
BstSLI GKGCMC 2 cut(s) 696, 1720
BstUI CGCG 1 cut(s) 50
BstX2I RGATCY 2 cut(s) 1357, 1689
BstYI RGATCY 2 cut(s) 1357, 1689
BsuRI GGCC 5 cut(s) 101, 141, 480, 767, 1445
BtrI CACGTC 2 cut(s) 39, 408
BtsCI GGATG 4 cut(s) 41, 463, 522, 788
BtsIMutI CAGTG 1 cut(s) 1718
BveI ACCTGC 1 cut(s) 278
Cac8I GCNNGC 3 cut(s) 681, 1216, 1533
CaiI CAGNNNCTG 1 cut(s) 1354
CciI TCATGA 1 cut(s) 640
CfoI GCGC 1 cut(s) 122
Cfr13I GGNCC 4 cut(s) 267, 479, 584, 765
Csp6I GTAC 3 cut(s) 1206, 1224, 1484
CviAII CATG 7 cut(s) 305, 603, 641, 891, 1165, 1298, 1441
CviQI GTAC 3 cut(s) 1206, 1224, 1484
DpnI GATC 4 cut(s) 228, 451, 1359, 1691
DpnII GATC 4 cut(s) 226, 449, 1357, 1689
DraI TTTAAA 1 cut(s) 421
DrdI GACNNNNNNGTC 1 cut(s) 431
DseDI GACNNNNNNGTC 1 cut(s) 431
EaeI YGGCCR 1 cut(s) 99
Eam1104I CTCTTC 2 cut(s) 840, 1663
EarI CTCTTC 2 cut(s) 840, 1663
EciI GGCGGA 1 cut(s) 1063
Eco31I GGTCTC 2 cut(s) 158, 1018
Eco32I GATATC 1 cut(s) 1153
Eco47I GGWCC 2 cut(s) 267, 584
Eco57I CTGAAG 2 cut(s) 515, 609
Eco88I CYCGRG 1 cut(s) 436
EcoRV GATATC 1 cut(s) 1153
EcoT22I ATGCAT 1 cut(s) 802
FaeI CATG 7 cut(s) 308, 606, 644, 894, 1168, 1301, 1444
FalI AAGNNNNNCTT 2 cut(s) 867, 899
FaqI GGGAC 3 cut(s) 283, 1395, 1478
FatI CATG 7 cut(s) 304, 602, 640, 890, 1164, 1297, 1440
FokI GGATG 4 cut(s) 28, 470, 509, 775
FspBI CTAG 9 cut(s) 200, 507, 648, 942, 1131, 1203, 1227, 1454, 1488
GlaI GCGC 1 cut(s) 121
GsuI CTGGAG 1 cut(s) 846
HaeIII GGCC 5 cut(s) 101, 141, 480, 767, 1445
HapII CCGG 1 cut(s) 98
HhaI GCGC 1 cut(s) 122
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 7 cut(s) 308, 606, 644, 894, 1168, 1301, 1444
Hin6I GCGC 1 cut(s) 120
HinP1I GCGC 1 cut(s) 120
HincII GTYRAC 2 cut(s) 1065, 1177
HindII GTYRAC 2 cut(s) 1065, 1177
HindIII AAGCTT 3 cut(s) 488, 1098, 1212
HpaII CCGG 1 cut(s) 98
HphI GGTGA 1 cut(s) 329
Hpy166II GTNNAC 4 cut(s) 267, 405, 1065, 1177
Hpy8I GTNNAC 4 cut(s) 267, 405, 1065, 1177
Hpy99I CGWCG 2 cut(s) 10, 843
HpyAV CCTTC 6 cut(s) 376, 539, 584, 801, 948, 1030
HpyCH4III ACNGT 6 cut(s) 80, 634, 786, 1311, 1399, 1437
HpyCH4IV ACGT 2 cut(s) 38, 407
HpyF10VI GCNNNNNNNGC 9 cut(s) 56, 279, 821, 1532, 1619, 1724, 1751, 1775, 1778
HpySE526I ACGT 2 cut(s) 38, 407
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 7 cut(s) 308, 606, 644, 894, 1168, 1301, 1444
HspAI GCGC 1 cut(s) 120
KpnI GGTACC 1 cut(s) 1487
Kzo9I GATC 4 cut(s) 226, 449, 1357, 1689
LmnI GCTCC 5 cut(s) 10, 242, 827, 940, 1504
LweI GCATC 3 cut(s) 787, 809, 1691
MaeI CTAG 9 cut(s) 200, 507, 648, 942, 1131, 1203, 1227, 1454, 1488
MaeII ACGT 2 cut(s) 38, 407
MaeIII GTNAC 1 cut(s) 1108
MalI GATC 4 cut(s) 228, 451, 1359, 1691
MbiI CCGCTC 3 cut(s) 188, 237, 1759
MboI GATC 4 cut(s) 226, 449, 1357, 1689
MboII GAAGA 7 cut(s) 79, 100, 126, 827, 1523, 1588, 1650
MflI RGATCY 2 cut(s) 1357, 1689
MhlI GDGCHC 2 cut(s) 696, 1720
MluCI AATT 9 cut(s) 515, 546, 595, 611, 946, 1121, 1140, 1571, 1856
MlyI GAGTC 6 cut(s) 107, 215, 253, 305, 1241, 1381
MmeI TCCRAC 2 cut(s) 195, 354
Mph1103I ATGCAT 1 cut(s) 802
MseI TTAA 3 cut(s) 420, 1275, 1876
MslI CAYNNNNRTG 2 cut(s) 753, 1866
MspA1I CMGCKG 5 cut(s) 503, 824, 955, 1754, 1775
MspI CCGG 1 cut(s) 98
MspR9I CCNGG 1 cut(s) 98
Mva1269I GAATGC 2 cut(s) 1324, 1477
MvnI CGCG 1 cut(s) 50
MwoI GCNNNNNNNGC 9 cut(s) 56, 279, 821, 1532, 1619, 1724, 1751, 1775, 1778
NciI CCSGG 1 cut(s) 98
NdeII GATC 4 cut(s) 226, 449, 1357, 1689
NlaIII CATG 7 cut(s) 308, 606, 644, 894, 1168, 1301, 1444
NlaIV GGNNCC 3 cut(s) 12, 1359, 1485
NmeAIII GCCGAG 2 cut(s) 1024, 1753
NsiI ATGCAT 1 cut(s) 802
NspV TTCGAA 1 cut(s) 90
PaeR7I CTCGAG 1 cut(s) 436
PagI TCATGA 1 cut(s) 640
PctI GAATGC 2 cut(s) 1324, 1477
PfeI GAWTC 5 cut(s) 342, 413, 578, 1514, 1846
PleI GAGTC 6 cut(s) 107, 214, 253, 304, 1240, 1381
PpsI GAGTC 6 cut(s) 107, 214, 253, 304, 1240, 1381
PshAI GACNNNNGTC 1 cut(s) 1070
PspN4I GGNNCC 3 cut(s) 12, 1359, 1485
PspPI GGNCC 4 cut(s) 267, 479, 584, 765
PstI CTGCAG 2 cut(s) 1774, 1780
PstNI CAGNNNCTG 1 cut(s) 1354
PsuI RGATCY 2 cut(s) 1357, 1689
PvuII CAGCTG 4 cut(s) 503, 955, 1754, 1775
RsaI GTAC 3 cut(s) 1207, 1225, 1485
RsaNI GTAC 3 cut(s) 1206, 1224, 1484
RseI CAYNNNNRTG 2 cut(s) 753, 1866
SaqAI TTAA 3 cut(s) 420, 1275, 1876
Sau3AI GATC 4 cut(s) 226, 449, 1357, 1689
Sau96I GGNCC 4 cut(s) 267, 479, 584, 765
ScaI AGTACT 1 cut(s) 1225
SchI GAGTC 6 cut(s) 107, 215, 253, 305, 1241, 1381
ScrFI CCNGG 1 cut(s) 98
SduI GDGCHC 2 cut(s) 696, 1720
SfaNI GCATC 3 cut(s) 787, 809, 1691
SfcI CTRYAG 2 cut(s) 1770, 1776
Sfr274I CTCGAG 1 cut(s) 436
SfuI TTCGAA 1 cut(s) 90
SinI GGWCC 2 cut(s) 267, 584
SlaI CTCGAG 1 cut(s) 436
SmiMI CAYNNNNRTG 2 cut(s) 753, 1866
SmlI CTYRAG 6 cut(s) 436, 1159, 1219, 1325, 1518, 1791
SmoI CTYRAG 6 cut(s) 436, 1159, 1219, 1325, 1518, 1791
Sse9I AATT 9 cut(s) 515, 546, 595, 611, 946, 1121, 1140, 1571, 1856
SspI AATATT 2 cut(s) 376, 1006
SspMI CTAG 9 cut(s) 200, 507, 648, 942, 1131, 1203, 1227, 1454, 1488
StyD4I CCNGG 1 cut(s) 96
TaaI ACNGT 6 cut(s) 80, 634, 786, 1311, 1399, 1437
TaiI ACGT 2 cut(s) 41, 410
TaqI TCGA 8 cut(s) 90, 111, 155, 162, 257, 294, 437, 551
TasI AATT 9 cut(s) 515, 546, 595, 611, 946, 1121, 1140, 1571, 1856
TatI WGTACW 2 cut(s) 1205, 1223
TauI GCSGC 4 cut(s) 59, 62, 251, 1751
TfiI GAWTC 5 cut(s) 342, 413, 578, 1514, 1846
Tru1I TTAA 3 cut(s) 420, 1275, 1876
Tru9I TTAA 3 cut(s) 420, 1275, 1876
TscAI CASTG 1 cut(s) 1725
TspGWI ACGGA 1 cut(s) 199
TspRI CASTG 1 cut(s) 1725
VpaK11BI GGWCC 2 cut(s) 267, 584
XapI RAATTY 2 cut(s) 546, 1856
XbaI TCTAGA 1 cut(s) 1453
XhoI CTCGAG 1 cut(s) 436
XspI CTAG 9 cut(s) 200, 507, 648, 942, 1131, 1203, 1227, 1454, 1488
ZrmI AGTACT 1 cut(s) 1225
Zsp2I ATGCAT 1 cut(s) 802
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.