FvH4_7g15510

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0016654
Physical Location & Seq
Forward (+)
13416603 .. 13418339
1737 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g15510.t1

Sequence Viewer

Length: 1434 bp
ATGAGGAAACCAAGTGTTGTGAAGTTGGGAACATGGAGAGGAGTTCAGAGGTCAATTTTCATATTGGGTAGTTTTGCTCTTCTCATCTCTATTGTTACATTAGCATCCAAATTCTACTCTCTCAAATCATTTCATGTTACTGATACCATCTGCAATTATGTAAATACTGATAGTCAAAGCAGGATTGGCAGTCATGTAAGAATCACTCTTGAAACTGTGATTCAGAAACTCCAACAGGAACTTAACAAGTTCAGAGATTCAACAACAGATTCGTCGTCATCTGCGCATTTGTTGAAGCACAGTTCGTTTCTTGCTGATATTTTGGGATTAGTCGAGTCGGTGCACGAATCTCTGGTGCAGAACGAAGGGACGGCTCAGAAGTTAGGCAATGATGTCAAAGACATCCATCCTCTGCTAAAACCGAAGCAACAATCCGATGAGCCTGCGGACTACTTTCTGATAGAAGAGATTCGGAAGTATGTCAAGATCAAACCGAACAGATTGGGAAAGCAGAATTTCATGGGAGCTAATGGGACTTTCACTAGCATTGGCCATGCTTGCTTTGCTATGAAGAAAGAACTTGAAGAATACATGACCTATGACATTGGTGATATCTGCAATGATGACTGGAAGCTAGCTCAGAAGCTCATGGTTCATGGATGTGATCCACTTCCGAGGAGGAGGTGCTTCTCTATTGCCCCAAAGATATATAGCAAGCCATTCCCAATTACTGAGTCCTTGTGGAAGCTCCCGGACAACAGAAATGTGCGGTGGAGTCACTATCGGTGCAAGAACTTTACTTGCCTCGCAAGTAATTCCGCTCCCAAGGGGTTCTTCAAATGTGCAGATTGCTTCAATCTGTCACACCATGAAATGCCTAGATGGATGAAACTAAATGCAGACCAGAATTTGAATCAGACTGCGGATTTTCTTATTCCTGAAGTGCTTCAGATCAAGCCGGGGGAGATCAGAATCGGACTGGATTTTAGCGTAGGCACGGGGACTTTTGCAGCTAGGATGAGGGAGTTCAATGTCACCATTGTCTCGGCAACAATCAATCTCGGGGCACCTTTCAACGAAATGATAGCTCTTCGTGGACTCGTCCCTCTTTACTTGACAATCAATCAAAGGCTACCATTCTTCGACAACACTTTGGATTTGATCCACACCACAAGATTTCTTGATGGCTGGATTGATTTTGTGCTGCTAGACTTTGTTCTGTTTGATTGGGATAGAGTTCTGAGGCCTGGGGGTTTGCTTTGGATTGACAGCTTCTTCTGTGTGAAGGAAGATTTGAATGATTATTTGGAGTCCCTGAAGATGCTGAGGTACAAGAAGCACAAATGGGTTATTGTTCCAAAGATTGATAAAGATGATGACAAAGAGGTTTTCTTTTCTGCTGTTCTAGAAAAGCCACCTAGACCATTCCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29 zf-RING_2

Protein Analysis

478

Amino Acids

54.86

Weight (kDa)

8.83

Isoelectric Point (pI)

37.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 316 - 440 2e-06 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016654)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G01240
fragaria_vesca FvH4_7g15510
malus_domestica MD07G1130500.v1.1
prunus_persica Prupe.2G178700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0357841
rosa_laevigata RLG00000027992
rosa_multiflora Rmu_sc0002071.1_g000002 Rmu_sc0011109.1_g000006
rosa_roxburghii Rroxscaffold_4G00298460
rosa_rugosa Rorug01G0258900
rosa_samantha Rh1AG272900 Rh1BG240200 Rh1DG268500
rosa_wichuraiana Rw1G024360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 285
AccB1I GGYRCC 1 cut(s) 1066
AccBSI CCGCTC 1 cut(s) 821
AciI CCGC 4 cut(s) 446, 769, 819, 923
AclWI GGATC 2 cut(s) 659, 1156
AcoI YGGCCR 1 cut(s) 550
AcsI RAATTY 3 cut(s) 110, 514, 907
AcuI CTGAAG 3 cut(s) 932, 960, 1337
AfaI GTAC 1 cut(s) 1331
AjnI CCWGG 1 cut(s) 1246
AjuI GAANNNNNNNTTGG 4 cut(s) 818, 850, 1289, 1321
AluBI AGCT 8 cut(s) 527, 634, 638, 646, 748, 1013, 1088, 1272
AluI AGCT 8 cut(s) 527, 634, 638, 646, 748, 1013, 1088, 1272
Alw21I GWGCWC 1 cut(s) 345
Alw26I GTCTC 1 cut(s) 1048
Alw44I GTGCAC 1 cut(s) 341
AlwI GGATC 2 cut(s) 659, 1156
Ama87I CYCGRG 1 cut(s) 1061
AoxI GGCC 2 cut(s) 550, 1244
ApaLI GTGCAC 1 cut(s) 341
ApeKI GCWGC 2 cut(s) 1010, 1204
ApoI RAATTY 3 cut(s) 110, 514, 907
Asp700I GAANNNNTTC 2 cut(s) 468, 945
AspLEI GCGC 1 cut(s) 286
AsuC2I CCSGG 2 cut(s) 752, 960
AsuHPI GGTGA 2 cut(s) 620, 1027
AsuNHI GCTAGC 1 cut(s) 634
AvaI CYCGRG 1 cut(s) 1061
BaeGI GKGCMC 2 cut(s) 345, 1069
BalI TGGCCA 1 cut(s) 552
BanI GGYRCC 1 cut(s) 1066
Bbv12I GWGCWC 1 cut(s) 345
BbvCI CCTCAGC 1 cut(s) 1325
BbvI GCAGC 2 cut(s) 1022, 1191
BccI CCATC 4 cut(s) 155, 414, 876, 1178
BceAI ACGGC 1 cut(s) 387
BcgI CGANNNNNNTGC 2 cut(s) 425, 459
BciT130I CCWGG 1 cut(s) 1248
BcnI CCSGG 2 cut(s) 752, 960
BcoDI GTCTC 1 cut(s) 1048
BfaI CTAG 7 cut(s) 543, 635, 879, 1014, 1208, 1406, 1419
BisI GCNGC 2 cut(s) 1011, 1205
BlsI GCNGC 2 cut(s) 1012, 1206
Bme1390I CCNGG 3 cut(s) 752, 960, 1248
BmeT110I CYCGRG 1 cut(s) 1061
BmiI GGNNCC 1 cut(s) 1068
BmrFI CCNGG 3 cut(s) 752, 960, 1248
BmsI GCATC 2 cut(s) 113, 1311
BmtI GCTAGC 1 cut(s) 638
Bpu10I CCTNAGC 1 cut(s) 1325
BpuMI CCSGG 2 cut(s) 752, 960
BsaBI GATNNNNATC 1 cut(s) 971
BsaJI CCNNGG 4 cut(s) 674, 825, 959, 1247
BsaWI WCCGGW 1 cut(s) 1428
Bse1I ACTGG 2 cut(s) 632, 984
Bse3DI GCAATG 2 cut(s) 394, 625
Bse8I GATNNNNATC 1 cut(s) 971
BseBI CCWGG 1 cut(s) 1248
BseDI CCNNGG 4 cut(s) 674, 825, 959, 1247
BseGI GGATG 6 cut(s) 104, 402, 406, 665, 891, 1023
BseJI GATNNNNATC 1 cut(s) 971
BseMI GCAATG 2 cut(s) 394, 625
BseMII CTCAG 5 cut(s) 389, 653, 723, 1232, 1316
BseNI ACTGG 2 cut(s) 632, 984
BseRI GAGGAG 3 cut(s) 54, 691, 694
BseSI GKGCMC 2 cut(s) 345, 1069
BseXI GCAGC 2 cut(s) 1022, 1191
BsgI GTGCAG 2 cut(s) 377, 864
BshFI GGCC 2 cut(s) 552, 1246
BshNI GGYRCC 1 cut(s) 1066
BsiHKAI GWGCWC 1 cut(s) 345
BsiHKCI CYCGRG 1 cut(s) 1061
BsiSI CCGG 3 cut(s) 752, 959, 1429
BslFI GGGAC 5 cut(s) 382, 547, 1015, 1088, 1297
BsmAI GTCTC 1 cut(s) 1048
BsmFI GGGAC 5 cut(s) 382, 547, 1015, 1088, 1297
BsnI GGCC 2 cut(s) 552, 1246
BsoBI CYCGRG 1 cut(s) 1061
Bsp1286I GDGCHC 2 cut(s) 345, 1069
Bsp143I GATC 5 cut(s) 486, 664, 951, 966, 1161
BspACI CCGC 4 cut(s) 446, 769, 819, 923
BspANI GGCC 2 cut(s) 552, 1246
BspCNI CTCAG 5 cut(s) 388, 652, 724, 1233, 1317
BspLI GGNNCC 1 cut(s) 1068
BspOI GCTAGC 1 cut(s) 638
BspPI GGATC 2 cut(s) 659, 1156
BspQI GCTCTTC 2 cut(s) 84, 1095
BspT107I GGYRCC 1 cut(s) 1066
BsrBI CCGCTC 1 cut(s) 821
BsrDI GCAATG 2 cut(s) 394, 625
BsrI ACTGG 2 cut(s) 632, 984
BssECI CCNNGG 4 cut(s) 674, 825, 959, 1247
BssMI GATC 5 cut(s) 486, 664, 951, 966, 1161
BssT1I CCWWGG 1 cut(s) 825
Bst2UI CCWGG 1 cut(s) 1248
Bst4CI ACNGT 2 cut(s) 217, 302
Bst6I CTCTTC 3 cut(s) 84, 459, 1095
BstC8I GCNNGC 4 cut(s) 444, 559, 636, 716
BstDEI CTNAG 5 cut(s) 375, 639, 732, 1241, 1325
BstF5I GGATG 6 cut(s) 104, 402, 406, 665, 891, 1023
BstHHI GCGC 1 cut(s) 286
BstKTI GATC 5 cut(s) 489, 667, 954, 969, 1164
BstMAI GTCTC 1 cut(s) 1048
BstMBI GATC 5 cut(s) 486, 664, 951, 966, 1161
BstMWI GCNNNNNNNGC 3 cut(s) 186, 558, 563
BstNI CCWGG 1 cut(s) 1248
BstSCI CCNGG 3 cut(s) 750, 958, 1246
BstSLI GKGCMC 2 cut(s) 345, 1069
BstV1I GCAGC 2 cut(s) 1022, 1191
BsuRI GGCC 2 cut(s) 552, 1246
BtsCI GGATG 6 cut(s) 104, 402, 406, 665, 891, 1023
Cac8I GCNNGC 4 cut(s) 444, 559, 636, 716
CfoI GCGC 1 cut(s) 286
Csp6I GTAC 1 cut(s) 1330
CviAII CATG 9 cut(s) 33, 134, 194, 520, 554, 592, 649, 656, 869
CviQI GTAC 1 cut(s) 1330
DdeI CTNAG 5 cut(s) 375, 639, 732, 1241, 1325
DpnI GATC 5 cut(s) 488, 666, 953, 968, 1163
DpnII GATC 5 cut(s) 486, 664, 951, 966, 1161
EaeI YGGCCR 1 cut(s) 550
Eam1104I CTCTTC 3 cut(s) 84, 459, 1095
EarI CTCTTC 3 cut(s) 84, 459, 1095
Eco130I CCWWGG 1 cut(s) 825
Eco147I AGGCCT 1 cut(s) 1246
Eco32I GATATC 1 cut(s) 613
Eco57I CTGAAG 3 cut(s) 932, 960, 1337
Eco88I CYCGRG 1 cut(s) 1061
EcoRII CCWGG 1 cut(s) 1246
EcoRV GATATC 1 cut(s) 613
EcoT14I CCWWGG 1 cut(s) 825
ErhI CCWWGG 1 cut(s) 825
FaeI CATG 9 cut(s) 36, 137, 197, 523, 557, 595, 652, 659, 872
FalI AAGNNNNNCTT 2 cut(s) 818, 850
FaqI GGGAC 5 cut(s) 382, 547, 1015, 1088, 1297
FatI CATG 9 cut(s) 32, 133, 193, 519, 553, 591, 648, 655, 868
Fnu4HI GCNGC 2 cut(s) 1011, 1205
FokI GGATG 6 cut(s) 91, 389, 393, 672, 898, 1030
Fsp4HI GCNGC 2 cut(s) 1011, 1205
FspBI CTAG 7 cut(s) 543, 635, 879, 1014, 1208, 1406, 1419
FspI TGCGCA 1 cut(s) 285
GlaI GCGC 1 cut(s) 285
GluI GCNGC 2 cut(s) 1011, 1205
HaeIII GGCC 2 cut(s) 552, 1246
HapII CCGG 3 cut(s) 752, 959, 1429
HhaI GCGC 1 cut(s) 286
Hin1II CATG 9 cut(s) 36, 137, 197, 523, 557, 595, 652, 659, 872
Hin6I GCGC 1 cut(s) 284
HinP1I GCGC 1 cut(s) 284
HpaII CCGG 3 cut(s) 752, 959, 1429
HphI GGTGA 2 cut(s) 620, 1027
Hpy166II GTNNAC 2 cut(s) 343, 1097
Hpy188III TCNNGA 5 cut(s) 209, 484, 938, 1181, 1406
Hpy8I GTNNAC 2 cut(s) 343, 1097
Hpy99I CGWCG 1 cut(s) 277
HpyAV CCTTC 2 cut(s) 359, 1279
HpyCH4III ACNGT 2 cut(s) 217, 302
HpyCH4V TGCA 8 cut(s) 153, 343, 358, 618, 789, 845, 899, 1010
HpyF10VI GCNNNNNNNGC 3 cut(s) 186, 558, 563
HpyF3I CTNAG 5 cut(s) 375, 639, 732, 1241, 1325
Hsp92II CATG 9 cut(s) 36, 137, 197, 523, 557, 595, 652, 659, 872
HspAI GCGC 1 cut(s) 284
Kzo9I GATC 5 cut(s) 486, 664, 951, 966, 1161
LguI GCTCTTC 2 cut(s) 84, 1095
LmnI GCTCC 3 cut(s) 524, 753, 826
Lsp1109I GCAGC 2 cut(s) 1022, 1191
LweI GCATC 2 cut(s) 113, 1311
MaeI CTAG 7 cut(s) 543, 635, 879, 1014, 1208, 1406, 1419
MaeIII GTNAC 5 cut(s) 94, 136, 776, 861, 1033
MalI GATC 5 cut(s) 488, 666, 953, 968, 1163
MbiI CCGCTC 1 cut(s) 821
MboI GATC 5 cut(s) 486, 664, 951, 966, 1161
MhlI GDGCHC 2 cut(s) 345, 1069
MlsI TGGCCA 1 cut(s) 552
MluCI AATT 7 cut(s) 54, 110, 154, 514, 726, 814, 907
MluNI TGGCCA 1 cut(s) 552
MlyI GAGTC 5 cut(s) 344, 743, 784, 1092, 1319
MmeI TCCRAC 1 cut(s) 256
Mox20I TGGCCA 1 cut(s) 552
MroXI GAANNNNTTC 2 cut(s) 468, 945
MscI TGGCCA 1 cut(s) 552
MseI TTAA 1 cut(s) 243
MslI CAYNNNNRTG 1 cut(s) 660
Msp20I TGGCCA 1 cut(s) 552
MspI CCGG 3 cut(s) 752, 959, 1429
MspR9I CCNGG 3 cut(s) 752, 960, 1248
MvaI CCWGG 1 cut(s) 1248
MwoI GCNNNNNNNGC 3 cut(s) 186, 558, 563
NciI CCSGG 2 cut(s) 752, 960
NdeII GATC 5 cut(s) 486, 664, 951, 966, 1161
NheI GCTAGC 1 cut(s) 634
NlaIII CATG 9 cut(s) 36, 137, 197, 523, 557, 595, 652, 659, 872
NlaIV GGNNCC 1 cut(s) 1068
NmeAIII GCCGAG 1 cut(s) 1025
NmuCI GTSAC 3 cut(s) 776, 861, 1033
NsbI TGCGCA 1 cut(s) 285
PceI AGGCCT 1 cut(s) 1246
PciSI GCTCTTC 2 cut(s) 84, 1095
PdmI GAANNNNTTC 2 cut(s) 468, 945
PfeI GAWTC 8 cut(s) 201, 220, 257, 269, 347, 469, 913, 972
PfoI TCCNGGA 1 cut(s) 750
PkrI GCNGC 2 cut(s) 1012, 1206
PleI GAGTC 5 cut(s) 343, 742, 783, 1092, 1318
PpsI GAGTC 5 cut(s) 343, 742, 783, 1092, 1318
Psp6I CCWGG 1 cut(s) 1246
PspGI CCWGG 1 cut(s) 1246
PspN4I GGNNCC 1 cut(s) 1068
RsaI GTAC 1 cut(s) 1331
RsaNI GTAC 1 cut(s) 1330
RseI CAYNNNNRTG 1 cut(s) 660
SapI GCTCTTC 2 cut(s) 84, 1095
SaqAI TTAA 1 cut(s) 243
SatI GCNGC 2 cut(s) 1011, 1205
Sau3AI GATC 5 cut(s) 486, 664, 951, 966, 1161
SchI GAGTC 5 cut(s) 344, 743, 784, 1092, 1319
ScrFI CCNGG 3 cut(s) 752, 960, 1248
SduI GDGCHC 2 cut(s) 345, 1069
SfaNI GCATC 2 cut(s) 113, 1311
SmiMI CAYNNNNRTG 1 cut(s) 660
Sse9I AATT 7 cut(s) 54, 110, 154, 514, 726, 814, 907
SseBI AGGCCT 1 cut(s) 1246
SsiI CCGC 4 cut(s) 446, 769, 819, 923
SspMI CTAG 7 cut(s) 543, 635, 879, 1014, 1208, 1406, 1419
StuI AGGCCT 1 cut(s) 1246
StyD4I CCNGG 3 cut(s) 750, 958, 1246
StyI CCWWGG 1 cut(s) 825
TaaI ACNGT 2 cut(s) 217, 302
TaqI TCGA 2 cut(s) 333, 1143
TasI AATT 7 cut(s) 54, 110, 154, 514, 726, 814, 907
TfiI GAWTC 8 cut(s) 201, 220, 257, 269, 347, 469, 913, 972
Tru1I TTAA 1 cut(s) 243
Tru9I TTAA 1 cut(s) 243
TseFI GTSAC 3 cut(s) 776, 861, 1033
TseI GCWGC 2 cut(s) 1010, 1204
Tsp45I GTSAC 3 cut(s) 776, 861, 1033
TspDTI ATGAA 7 cut(s) 49, 122, 508, 584, 644, 885, 902
VneI GTGCAC 1 cut(s) 341
XapI RAATTY 3 cut(s) 110, 514, 907
XbaI TCTAGA 1 cut(s) 1405
XmnI GAANNNNTTC 2 cut(s) 468, 945
XspI CTAG 7 cut(s) 543, 635, 879, 1014, 1208, 1406, 1419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.