MD07G1130500.v1.1

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0016654
Physical Location & Seq
Reverse (-)
18644894 .. 18648340
3447 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1130500.v1.1.491

Sequence Viewer

Length: 2109 bp
ATGTCCTCCTCCAACTCCACCTCCCACTCCCCCACTAATTACCTAACTACCCTCGGCCTCGGCTACGGCATCGCCATTTCCCTCGGCTTCCTCGTCCTCTTCTCCACCCTCCTCCTCGCCTCCTACCTCTGCTGCCGCGTCTCCCGCCACCGCCCTCCTCAGCCCCGAAACCCCAATCCGAATCCGAACCCCGAGGGCGTCATCCTTCCCCGCATCATCTTCGTCTCCGAAGACGACAACGACCAGGAGCAGCAGGACGACGATGAGAACGCCGTCGTCGGGCTCCACCCGAGCGTCATCAACTCCTACCCGAAATTCCCGTTCTCCAAGGAGGCGGCGACCCCCGATTCGTCCACTTGCTCCATCTGCCTGTGCGACTACAAGGACGCAGAGATGCTCCGGATGATGCCGGAGTGCAGGCACTACTTCCACCTGTTGTGCCTCGACGCGTGGCTCCGGCTCAACGGGTCGTGTCCAGTTTGCCGGAACTCGCCGCTTCCAACGCCTCTGTCGACTCCGTTGCAGGAGGTCGTGCCTTTGTCTCAGTACCCAGCCGATCGGGGATGGAGTAGAATTGTGTTAGATTTTGAATGGAGTGTGGGTGGATTGAATTGTTGCTTGGTTTGGGGAGATTGGGAAATGTACATTTTTTGGGATTGTGAAATTGATATGAGGAAACCAAATGTTTTCAAGAACTTGGGAACATGGAGAGGAAGAGTTCGCAGCTCAATCTTTGCATTAAGTGCTTTTGCTCTTCTCATCTCTGTTGTTACTTTATCCAAATTCAACTCTATCGGATCGTTTCACGTCACCGATACGCTCTGCGGTTACGTAAATTCTGATCAGAGTCCAAGCAGGAGCGATGGCCATGTCACAAAAACGCTTGAAACTGTAATCCAGAAAATCGAAACAGAACTTAAGAATTTGAGAGATTCGCAACCAGAGGCGTCACACGGACTTGTGTTGAAATACAGTTCCTTTCTTGCTGATGTTCTGGGGCTTATTGAGTCAGTGCAGACATCTCTGTCACAGAATGAAGAGACTCACAAGGTAAACAGTGGGAGCGAAGACATTCATCCGCTCTTAAAACCGAAGCAGCAATCCGATGAACCTGCTAATTACTTTCTGACCGAAGAGATTCGAAAGTACGTCAAGATCAAGCCGAACAGATTAGGGAAGCAGAATTTCATGGGAGCCAATGGGACTTTCACCAGCATCGGCCATGCTTGCTTTGCTATGAAGAAAGAGCTCGAAGAGTACATGGACTATGACATCGGCGACATTTGCAACGATGACTGGAGGCTGGCTCAGAAGCTGATGATTCACGGATGTGATCCGCTTCCGAGGAGGAGGTGCTTCTCCAGAGCCCCAAAGCTATACAGCACGCCGTTTCCAATTCGTGAGTCCATGTGGAAGCTCCCTGATGATAGAAATGTCCGCTGGAGTAACTACATTTGCAAAAACTTTACTTGCCTCGCAAGCAACAAAACTCGGAAGGGGTTCTTCAAATGTGCAGATTGCTTCAATCTTGCAGGCCACGAAAAGCCAAGATGGATCCAACCAGCAAACCTAGAAGACTCCAATTCGAATGTAACCACAGATTTTCTCATCCCTGAAGTGCTCGAAATCAAGCCCGGGGAGATCAGGATTGGGCTCGATTTCAGCGTAGGAACTGGGACTTTTGCAGCAAGGATGAGGGAGTTCAATGTGACTATAGTCTCGGCAACTATCAATCTTGGAGCACCTTTCAACGAAATGATAGCGCTGCGTGGACTTGTTCCGCTTTACCTGACAGTAAATCAACGGCTTCCATTTTTCGATAACACCCTTGATTTGATCCACACCACGAGGTTTCTAGACGGATGGATAGATCTCGTGCTACTGGACTTTGTTCTTTATGATTGGGACAGAGTTCTGAGGCCGGGCGGATTCTTGTGGATCGACAGCTTCTTTTGTTTGAAGGAGGATTTGAGTGATTATTTGGAATCCCTGAAGATGCTGAGGTACAAGAAGCACAAATGGGTTGTTGTTCCAAAGCTTGATAAAAGTGATGATAAGGAAGTTTTCTTCTCTGCTCTATTAGAGAAGCCGGGAAGGCCGTTCCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29 zf-RING_2

Protein Analysis

703

Amino Acids

79.96

Weight (kDa)

6.15

Isoelectric Point (pI)

44.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 119 - 162 1.1e-11 Ring finger domain
zf-RING_11 PF17123 120 - 148 6.7e-07 RING-like zinc finger
zf-rbx1 PF12678 125 - 162 3.6e-07 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016654)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G01240
fragaria_vesca FvH4_7g15510
malus_domestica MD07G1130500.v1.1
prunus_persica Prupe.2G178700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0357841
rosa_laevigata RLG00000027992
rosa_multiflora Rmu_sc0002071.1_g000002 Rmu_sc0011109.1_g000006
rosa_roxburghii Rroxscaffold_4G00298460
rosa_rugosa Rorug01G0258900
rosa_samantha Rh1AG272900 Rh1BG240200 Rh1DG268500
rosa_wichuraiana Rw1G024360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1024
Acc36I ACCTGC 1 cut(s) 1120
AccBSI CCGCTC 1 cut(s) 1081
AccI GTMKAC 1 cut(s) 512
AccII CGCG 2 cut(s) 138, 449
AccIII TCCGGA 1 cut(s) 399
AclWI GGATC 6 cut(s) 805, 1328, 1549, 1562, 1831, 1946
AcoI YGGCCR 2 cut(s) 865, 1219
AcsI RAATTY 5 cut(s) 314, 782, 835, 922, 1183
AcuI CTGAAG 2 cut(s) 1635, 2012
AcyI GRCGYC 2 cut(s) 198, 947
AfaI GTAC 5 cut(s) 548, 644, 1148, 1259, 2006
AfeI AGCGCT 1 cut(s) 1764
AfiI CCNNNNNNNGG 1 cut(s) 279
AflII CTTAAG 1 cut(s) 917
AflIII ACRYGT 1 cut(s) 447
AjiI CACGTC 1 cut(s) 808
AjnI CCWGG 1 cut(s) 243
AjuI GAANNNNNNNTTGG 4 cut(s) 602, 634, 1190, 1222
AleI CACNNNNGTG 1 cut(s) 1329
AluBI AGCT 7 cut(s) 726, 1249, 1315, 1375, 1417, 1947, 2038
AluI AGCT 7 cut(s) 726, 1249, 1315, 1375, 1417, 1947, 2038
Alw21I GWGCWC 3 cut(s) 1251, 1623, 1744
Alw26I GTCTC 5 cut(s) 145, 229, 546, 1034, 1723
AlwI GGATC 6 cut(s) 805, 1328, 1549, 1562, 1831, 1946
AlwNI CAGNNNCTG 1 cut(s) 1315
Ama87I CYCGRG 3 cut(s) 191, 289, 1634
Aor13HI TCCGGA 1 cut(s) 399
Aor51HI AGCGCT 1 cut(s) 1764
AoxI GGCC 6 cut(s) 55, 865, 1219, 1534, 1919, 2096
ApeKI GCWGC 6 cut(s) 132, 250, 723, 1096, 1685, 1765
ApoI RAATTY 5 cut(s) 314, 782, 835, 922, 1183
ArsI GACNNNNNNTTYG 2 cut(s) 331, 363
Asp700I GAANNNNTTC 3 cut(s) 1071, 1137, 1499
AspLEI GCGC 1 cut(s) 1765
AsuC2I CCSGG 4 cut(s) 1635, 1636, 1923, 2091
AsuHPI GGTGA 2 cut(s) 802, 1201
AsuII TTCGAA 2 cut(s) 1141, 1586
AvaI CYCGRG 3 cut(s) 191, 289, 1634
BalI TGGCCA 1 cut(s) 867
BamHI GGATCC 1 cut(s) 1554
BanII GRGCYC 4 cut(s) 285, 1251, 1369, 1656
BauI CACGAG 2 cut(s) 1846, 1874
BbsI GAAGAC 3 cut(s) 237, 1074, 1581
Bbv12I GWGCWC 3 cut(s) 1251, 1623, 1744
BbvCI CCTCAGC 2 cut(s) 159, 2000
BbvI GCAGC 6 cut(s) 119, 262, 735, 1108, 1697, 1752
BccI CCATC 5 cut(s) 371, 558, 857, 1545, 1857
BceAI ACGGC 5 cut(s) 82, 257, 1372, 1820, 2083
BcgI CGANNNNNNTGC 6 cut(s) 202, 236, 502, 536, 1094, 1128
BciT130I CCWGG 1 cut(s) 245
BclI TGATCA 1 cut(s) 841
BcnI CCSGG 4 cut(s) 1635, 1636, 1923, 2091
BcoDI GTCTC 5 cut(s) 145, 229, 546, 1034, 1723
BfaI CTAG 2 cut(s) 1571, 1856
BfmI CTRYAG 1 cut(s) 1713
BfoI RGCGCY 1 cut(s) 1766
BfrI CTTAAG 1 cut(s) 917
BfuAI ACCTGC 1 cut(s) 1120
BglI GCCNNNNNGGC 1 cut(s) 2095
BglII AGATCT 1 cut(s) 1870
BisI GCNGC 9 cut(s) 133, 136, 251, 336, 494, 724, 1097, 1686, 1766
BlsI GCNGC 9 cut(s) 134, 137, 252, 337, 495, 725, 1098, 1687, 1767
Bme1390I CCNGG 5 cut(s) 245, 1635, 1636, 1923, 2091
BmeT110I CYCGRG 3 cut(s) 191, 289, 1634
BmgBI CACGTC 1 cut(s) 808
BmiI GGNNCC 4 cut(s) 284, 455, 1195, 1556
BmrFI CCNGG 5 cut(s) 245, 1635, 1636, 1923, 2091
BmrI ACTGGG 1 cut(s) 1683
BmsI GCATC 6 cut(s) 78, 222, 384, 396, 1224, 1986
BmuI ACTGGG 1 cut(s) 1683
BoxI GACNNNNGTC 1 cut(s) 1715
BpiI GAAGAC 3 cut(s) 237, 1074, 1581
BplI GAGNNNNNCTC 2 cut(s) 1291, 1323
BpmI CTGGAG 3 cut(s) 1318, 1345, 1462
Bpu10I CCTNAGC 2 cut(s) 159, 2000
Bpu14I TTCGAA 2 cut(s) 1141, 1586
BpuMI CCSGG 4 cut(s) 1635, 1636, 1923, 2091
BsaAI YACGTR 1 cut(s) 832
BsaHI GRCGYC 2 cut(s) 198, 947
BsaJI CCNNGG 8 cut(s) 52, 58, 82, 192, 327, 1343, 1634, 1635
BsaWI WCCGGW 1 cut(s) 399
BsaXI ACNNNNNCTCC 4 cut(s) 5, 35, 586, 616
Bsc4I CCNNNNNNNGG 1 cut(s) 279
Bse1I ACTGG 4 cut(s) 476, 1301, 1678, 1887
BseAI TCCGGA 1 cut(s) 399
BseBI CCWGG 1 cut(s) 245
BseDI CCNNGG 8 cut(s) 52, 58, 82, 192, 327, 1343, 1634, 1635
BseGI GGATG 8 cut(s) 201, 408, 569, 1075, 1334, 1608, 1698, 1868
BseLI CCNNNNNNNGG 1 cut(s) 279
BseMII CTCAG 5 cut(s) 173, 557, 1322, 1907, 1991
BseNI ACTGG 4 cut(s) 476, 1301, 1678, 1887
BseRI GAGGAG 5 cut(s) 101, 104, 147, 1360, 1363
BseXI GCAGC 6 cut(s) 119, 262, 735, 1108, 1697, 1752
BseYI CCCAGC 1 cut(s) 550
BsgI GTGCAG 3 cut(s) 436, 1034, 1533
Bsh1236I CGCG 2 cut(s) 138, 449
Bsh1285I CGRYCG 1 cut(s) 559
BshFI GGCC 6 cut(s) 57, 867, 1221, 1536, 1921, 2098
BsiEI CGRYCG 1 cut(s) 559
BsiHKAI GWGCWC 3 cut(s) 1251, 1623, 1744
BsiHKCI CYCGRG 3 cut(s) 191, 289, 1634
BsiSI CCGG 7 cut(s) 400, 410, 457, 484, 1635, 1922, 2090
BslFI GGGAC 3 cut(s) 1216, 1690, 1919
BslI CCNNNNNNNGG 1 cut(s) 279
BsmAI GTCTC 5 cut(s) 145, 229, 546, 1034, 1723
BsmBI CGTCTC 2 cut(s) 145, 229
BsmFI GGGAC 3 cut(s) 1216, 1690, 1919
BsnI GGCC 6 cut(s) 57, 867, 1221, 1536, 1921, 2098
BsoBI CYCGRG 3 cut(s) 191, 289, 1634
Bsp119I TTCGAA 2 cut(s) 1141, 1586
Bsp1286I GDGCHC 6 cut(s) 285, 1251, 1369, 1623, 1656, 1744
Bsp13I TCCGGA 1 cut(s) 399
Bsp1407I TGTACA 1 cut(s) 642
BspANI GGCC 6 cut(s) 57, 867, 1221, 1536, 1921, 2098
BspCNI CTCAG 5 cut(s) 172, 556, 1321, 1908, 1992
BspEI TCCGGA 1 cut(s) 399
BspFNI CGCG 2 cut(s) 138, 449
BspLI GGNNCC 4 cut(s) 284, 455, 1195, 1556
BspMI ACCTGC 1 cut(s) 1120
BspPI GGATC 6 cut(s) 805, 1328, 1549, 1562, 1831, 1946
BspQI GCTCTTC 1 cut(s) 759
BspT104I TTCGAA 2 cut(s) 1141, 1586
BspTI CTTAAG 1 cut(s) 917
BsrBI CCGCTC 1 cut(s) 1081
BsrGI TGTACA 1 cut(s) 642
BsrI ACTGG 4 cut(s) 476, 1301, 1678, 1887
BssECI CCNNGG 8 cut(s) 52, 58, 82, 192, 327, 1343, 1634, 1635
BssNI GRCGYC 2 cut(s) 198, 947
BssSI CACGAG 2 cut(s) 1846, 1874
BssT1I CCWWGG 1 cut(s) 327
Bst2BI CACGAG 2 cut(s) 1846, 1874
Bst2UI CCWGG 1 cut(s) 245
Bst4CI ACNGT 4 cut(s) 892, 974, 1058, 1795
Bst6I CTCTTC 6 cut(s) 104, 709, 759, 1032, 1128, 1248
BstACI GRCGYC 2 cut(s) 198, 947
BstAFI CTTAAG 1 cut(s) 917
BstAPI GCANNNNNTGC 1 cut(s) 743
BstAUI TGTACA 1 cut(s) 642
BstBAI YACGTR 1 cut(s) 832
BstBI TTCGAA 2 cut(s) 1141, 1586
BstC8I GCNNGC 6 cut(s) 419, 1228, 1305, 1385, 1480, 1534
BstDEI CTNAG 5 cut(s) 159, 543, 1308, 1916, 2000
BstF5I GGATG 8 cut(s) 201, 408, 569, 1075, 1334, 1608, 1698, 1868
BstFNI CGCG 2 cut(s) 138, 449
BstH2I RGCGCY 1 cut(s) 1766
BstHHI GCGC 1 cut(s) 1765
BstMAI GTCTC 5 cut(s) 145, 229, 546, 1034, 1723
BstMCI CGRYCG 1 cut(s) 559
BstMWI GCNNNNNNNGC 9 cut(s) 144, 366, 502, 743, 1227, 1232, 1284, 1479, 2095
BstNI CCWGG 1 cut(s) 245
BstPAI GACNNNNGTC 1 cut(s) 1715
BstSCI CCNGG 5 cut(s) 243, 1633, 1634, 1921, 2089
BstSFI CTRYAG 1 cut(s) 1713
BstSNI TACGTA 1 cut(s) 832
BstUI CGCG 2 cut(s) 138, 449
BstV1I GCAGC 6 cut(s) 119, 262, 735, 1108, 1697, 1752
BstV2I GAAGAC 3 cut(s) 237, 1074, 1581
BstX2I RGATCY 2 cut(s) 1554, 1870
BstYI RGATCY 2 cut(s) 1554, 1870
BsuRI GGCC 6 cut(s) 57, 867, 1221, 1536, 1921, 2098
BtgZI GCGATG 2 cut(s) 55, 876
BtrI CACGTC 1 cut(s) 808
BtsCI GGATG 8 cut(s) 201, 408, 569, 1075, 1334, 1608, 1698, 1868
BtsIMutI CAGTG 2 cut(s) 1017, 1063
BveI ACCTGC 1 cut(s) 1120
Cac8I GCNNGC 6 cut(s) 419, 1228, 1305, 1385, 1480, 1534
CaiI CAGNNNCTG 1 cut(s) 1315
CfoI GCGC 1 cut(s) 1765
Cfr9I CCCGGG 1 cut(s) 1634
CseI GACGC 6 cut(s) 127, 187, 283, 395, 455, 936
Csp6I GTAC 5 cut(s) 547, 643, 1147, 1258, 2005
CviAII CATG 6 cut(s) 705, 869, 1189, 1223, 1261, 1408
CviQI GTAC 5 cut(s) 547, 643, 1147, 1258, 2005
DdeI CTNAG 5 cut(s) 159, 543, 1308, 1916, 2000
DrdI GACNNNNNNGTC 1 cut(s) 1024
DseDI GACNNNNNNGTC 1 cut(s) 1024
EaeI YGGCCR 2 cut(s) 865, 1219
Eam1104I CTCTTC 6 cut(s) 104, 709, 759, 1032, 1128, 1248
EarI CTCTTC 6 cut(s) 104, 709, 759, 1032, 1128, 1248
EciI GGCGGA 1 cut(s) 1941
Ecl136II GAGCTC 1 cut(s) 1249
Eco105I TACGTA 1 cut(s) 832
Eco130I CCWWGG 1 cut(s) 327
Eco24I GRGCYC 4 cut(s) 285, 1251, 1369, 1656
Eco47III AGCGCT 1 cut(s) 1764
Eco53kI GAGCTC 1 cut(s) 1249
Eco57I CTGAAG 2 cut(s) 1635, 2012
Eco88I CYCGRG 3 cut(s) 191, 289, 1634
EcoICRI GAGCTC 1 cut(s) 1249
EcoRII CCWGG 1 cut(s) 243
EcoT14I CCWWGG 1 cut(s) 327
EcoT38I GRGCYC 4 cut(s) 285, 1251, 1369, 1656
ErhI CCWWGG 1 cut(s) 327
Esp3I CGTCTC 2 cut(s) 145, 229
FaeI CATG 6 cut(s) 708, 872, 1192, 1226, 1264, 1411
FalI AAGNNNNNCTT 2 cut(s) 1487, 1519
FaqI GGGAC 3 cut(s) 1216, 1690, 1919
FatI CATG 6 cut(s) 704, 868, 1188, 1222, 1260, 1407
FauI CCCGC 2 cut(s) 152, 218
FbaI TGATCA 1 cut(s) 841
FblI GTMKAC 1 cut(s) 512
Fnu4HI GCNGC 9 cut(s) 133, 136, 251, 336, 494, 724, 1097, 1686, 1766
FokI GGATG 8 cut(s) 188, 415, 576, 1062, 1341, 1595, 1705, 1875
FriOI GRGCYC 4 cut(s) 285, 1251, 1369, 1656
Fsp4HI GCNGC 9 cut(s) 133, 136, 251, 336, 494, 724, 1097, 1686, 1766
FspBI CTAG 2 cut(s) 1571, 1856
GlaI GCGC 1 cut(s) 1764
GluI GCNGC 9 cut(s) 133, 136, 251, 336, 494, 724, 1097, 1686, 1766
GsaI CCCAGC 1 cut(s) 554
GsuI CTGGAG 3 cut(s) 1318, 1345, 1462
HaeII RGCGCY 1 cut(s) 1766
HaeIII GGCC 6 cut(s) 57, 867, 1221, 1536, 1921, 2098
HapII CCGG 7 cut(s) 400, 410, 457, 484, 1635, 1922, 2090
HgaI GACGC 6 cut(s) 127, 187, 283, 395, 455, 936
HhaI GCGC 1 cut(s) 1765
Hin1I GRCGYC 2 cut(s) 198, 947
Hin1II CATG 6 cut(s) 708, 872, 1192, 1226, 1264, 1411
Hin6I GCGC 1 cut(s) 1763
HinP1I GCGC 1 cut(s) 1763
HincII GTYRAC 1 cut(s) 513
HindII GTYRAC 1 cut(s) 513
HindIII AAGCTT 1 cut(s) 2036
HpaII CCGG 7 cut(s) 400, 410, 457, 484, 1635, 1922, 2090
HphI GGTGA 2 cut(s) 802, 1201
Hpy166II GTNNAC 4 cut(s) 354, 513, 1054, 1772
Hpy188III TCNNGA 8 cut(s) 400, 691, 898, 1153, 1362, 1400, 1645, 1856
Hpy8I GTNNAC 4 cut(s) 354, 513, 1054, 1772
Hpy99I CGWCG 4 cut(s) 263, 278, 281, 449
HpyAV CCTTC 4 cut(s) 215, 1489, 1954, 2088
HpyCH4III ACNGT 4 cut(s) 892, 974, 1058, 1795
HpyCH4IV ACGT 3 cut(s) 807, 831, 1149
HpyCH4V TGCA 9 cut(s) 417, 523, 737, 1015, 1287, 1458, 1514, 1532, 1685
HpyF10VI GCNNNNNNNGC 9 cut(s) 144, 366, 502, 743, 1227, 1232, 1284, 1479, 2095
HpyF3I CTNAG 5 cut(s) 159, 543, 1308, 1916, 2000
HpySE526I ACGT 3 cut(s) 807, 831, 1149
Hsp92I GRCGYC 2 cut(s) 198, 947
Hsp92II CATG 6 cut(s) 708, 872, 1192, 1226, 1264, 1411
HspAI GCGC 1 cut(s) 1763
Kpn2I TCCGGA 1 cut(s) 399
Ksp22I TGATCA 1 cut(s) 841
LguI GCTCTTC 1 cut(s) 759
Lsp1109I GCAGC 6 cut(s) 119, 262, 735, 1108, 1697, 1752
LweI GCATC 6 cut(s) 78, 222, 384, 396, 1224, 1986
MaeI CTAG 2 cut(s) 1571, 1856
MaeII ACGT 3 cut(s) 807, 831, 1149
MaeIII GTNAC 9 cut(s) 769, 808, 827, 871, 948, 1026, 1445, 1591, 1708
MbiI CCGCTC 1 cut(s) 1081
MflI RGATCY 2 cut(s) 1554, 1870
MhlI GDGCHC 6 cut(s) 285, 1251, 1369, 1623, 1656, 1744
MlsI TGGCCA 1 cut(s) 867
MluI ACGCGT 1 cut(s) 447
MluNI TGGCCA 1 cut(s) 867
MlyI GAGTC 6 cut(s) 508, 856, 1016, 1036, 1412, 1571
MmeI TCCRAC 3 cut(s) 36, 524, 1582
Mox20I TGGCCA 1 cut(s) 867
MroI TCCGGA 1 cut(s) 399
MroXI GAANNNNTTC 3 cut(s) 1071, 1137, 1499
MscI TGGCCA 1 cut(s) 867
MseI TTAA 3 cut(s) 740, 918, 1085
MslI CAYNNNNRTG 1 cut(s) 1329
Msp20I TGGCCA 1 cut(s) 867
MspA1I CMGCKG 1 cut(s) 1440
MspCI CTTAAG 1 cut(s) 917
MspI CCGG 7 cut(s) 400, 410, 457, 484, 1635, 1922, 2090
MspR9I CCNGG 5 cut(s) 245, 1635, 1636, 1923, 2091
MvaI CCWGG 1 cut(s) 245
MvnI CGCG 2 cut(s) 138, 449
MwoI GCNNNNNNNGC 9 cut(s) 144, 366, 502, 743, 1227, 1232, 1284, 1479, 2095
NciI CCSGG 4 cut(s) 1635, 1636, 1923, 2091
NlaIII CATG 6 cut(s) 708, 872, 1192, 1226, 1264, 1411
NlaIV GGNNCC 4 cut(s) 284, 455, 1195, 1556
NmeAIII GCCGAG 4 cut(s) 33, 39, 63, 1700
NmuCI GTSAC 5 cut(s) 808, 871, 948, 1026, 1708
NspV TTCGAA 2 cut(s) 1141, 1586
OliI CACNNNNGTG 1 cut(s) 1329
PciSI GCTCTTC 1 cut(s) 759
PcsI WCGNNNNNNNCGW 3 cut(s) 90, 509, 1662
PdmI GAANNNNTTC 3 cut(s) 1071, 1137, 1499
PfeI GAWTC 7 cut(s) 181, 347, 932, 1138, 1321, 1929, 1985
PkrI GCNGC 9 cut(s) 134, 137, 252, 337, 495, 725, 1098, 1687, 1767
Ple19I CGATCG 1 cut(s) 559
PleI GAGTC 6 cut(s) 508, 855, 1015, 1036, 1411, 1571
PpsI GAGTC 6 cut(s) 508, 855, 1015, 1036, 1411, 1571
Ppu21I YACGTR 1 cut(s) 832
PshAI GACNNNNGTC 1 cut(s) 1715
Psp124BI GAGCTC 1 cut(s) 1251
Psp6I CCWGG 1 cut(s) 243
PspFI CCCAGC 1 cut(s) 550
PspGI CCWGG 1 cut(s) 243
PspN4I GGNNCC 4 cut(s) 284, 455, 1195, 1556
PstNI CAGNNNCTG 1 cut(s) 1315
PsuI RGATCY 2 cut(s) 1554, 1870
PvuI CGATCG 1 cut(s) 559
RsaI GTAC 5 cut(s) 548, 644, 1148, 1259, 2006
RsaNI GTAC 5 cut(s) 547, 643, 1147, 1258, 2005
RseI CAYNNNNRTG 1 cut(s) 1329
SacI GAGCTC 1 cut(s) 1251
SalI GTCGAC 1 cut(s) 511
SapI GCTCTTC 1 cut(s) 759
SaqAI TTAA 3 cut(s) 740, 918, 1085
SatI GCNGC 9 cut(s) 133, 136, 251, 336, 494, 724, 1097, 1686, 1766
SchI GAGTC 6 cut(s) 508, 856, 1016, 1036, 1412, 1571
ScrFI CCNGG 5 cut(s) 245, 1635, 1636, 1923, 2091
SduI GDGCHC 6 cut(s) 285, 1251, 1369, 1623, 1656, 1744
SfaNI GCATC 6 cut(s) 78, 222, 384, 396, 1224, 1986
SfcI CTRYAG 1 cut(s) 1713
SfuI TTCGAA 2 cut(s) 1141, 1586
SmaI CCCGGG 1 cut(s) 1636
SmiMI CAYNNNNRTG 1 cut(s) 1329
SmlI CTYRAG 1 cut(s) 917
SmoI CTYRAG 1 cut(s) 917
SnaBI TACGTA 1 cut(s) 832
SspMI CTAG 2 cut(s) 1571, 1856
SstI GAGCTC 1 cut(s) 1251
StyD4I CCNGG 5 cut(s) 243, 1633, 1634, 1921, 2089
StyI CCWWGG 1 cut(s) 327
TaaI ACNGT 4 cut(s) 892, 974, 1058, 1795
TaiI ACGT 3 cut(s) 810, 834, 1152
TaqII GACCGA 1 cut(s) 1145
TatI WGTACW 2 cut(s) 642, 1257
TauI GCSGC 3 cut(s) 138, 338, 496
TfiI GAWTC 7 cut(s) 181, 347, 932, 1138, 1321, 1929, 1985
Tru1I TTAA 3 cut(s) 740, 918, 1085
Tru9I TTAA 3 cut(s) 740, 918, 1085
TscAI CASTG 2 cut(s) 1017, 1063
TseFI GTSAC 5 cut(s) 808, 871, 948, 1026, 1708
TseI GCWGC 6 cut(s) 132, 250, 723, 1096, 1685, 1765
Tsp45I GTSAC 5 cut(s) 808, 871, 948, 1026, 1708
TspDTI ATGAA 5 cut(s) 1050, 1064, 1122, 1177, 1253
TspGWI ACGGA 5 cut(s) 507, 969, 1341, 1875, 2093
TspMI CCCGGG 1 cut(s) 1634
TspRI CASTG 2 cut(s) 1017, 1063
Vha464I CTTAAG 1 cut(s) 917
XapI RAATTY 5 cut(s) 314, 782, 835, 922, 1183
XbaI TCTAGA 1 cut(s) 1855
XmaI CCCGGG 1 cut(s) 1634
XmiI GTMKAC 1 cut(s) 512
XmnI GAANNNNTTC 3 cut(s) 1071, 1137, 1499
XspI CTAG 2 cut(s) 1571, 1856
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.