Rorug01G0258900

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0016654
Physical Location & Seq
Reverse (-)
36832864 .. 36833677
814 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0258900.1

Sequence Viewer

Length: 729 bp
ATGGAAGAGAAGCCCTCAAAAACCCTCAATTTGATCGACAAGTTAATGCATGCAGCAGAATCGCCCTCAACTGCTAGTAGTAGCAGAACCAGTACTGCAGGAGACAACCAACAACTCTGTGATGCAGCCACTGCTTTAACTTTGCATCAATCTTGGTTGCAGCCGGCATCGACCACAAACAAGAGAAAAGAAAAAGCACCAGTTATACCGGAAAAGGAAGACAGAATCGAGAAACCAGCCGCTCCTACTAATCCGAAACATCAAAAACTAGTTCTCTCTGATCATGAGAATATAGAGTTGAAGAAGAAACGTGACCCAGTGGCTCGTGACCCAGCCTTATACAAAATAAAGAAGAAGTTGACTCTAAGTGACCTTGGAAACTTGAGCAGGCTCTTGGTGCCGAAAGAGATGGTCAAGAAACATGTTCTGCCTTCGGTGGATGCCGACTTTGTACAAAGACTCGAGAGCAAAGCCGGTAAACAAGTTACGGTTAAAGACTCTGACAAAGAGACCGAGCATCAATTAACTTTTAGATACTTGAGGTCGTCGAAGAGTTATGTTTTCAATGGAAATTGGATGGAAATCGTGAAGGGAAGAGATTTGAAGAAGGATGATGAAATTGGACTTTACTGGGACACTCTCAATTCCAAGTTCCACTTTTCTCTTCTGAAAGTTGCAAGCCAAGAAAGCCAAGCTGCCGATGAAGTTACTCGTGTTCATCAACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29 zf-RING_2

Protein Analysis

242

Amino Acids

27.41

Weight (kDa)

9.27

Isoelectric Point (pI)

45.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 118 - 221 1.2e-07 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016654)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G01240
fragaria_vesca FvH4_7g15510
malus_domestica MD07G1130500.v1.1
prunus_persica Prupe.2G178700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0357841
rosa_laevigata RLG00000027992
rosa_multiflora Rmu_sc0002071.1_g000002 Rmu_sc0011109.1_g000006
rosa_roxburghii Rroxscaffold_4G00298460
rosa_rugosa Rorug01G0258900
rosa_samantha Rh1AG272900 Rh1BG240200 Rh1DG268500
rosa_wichuraiana Rw1G024360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 397
AccBSI CCGCTC 1 cut(s) 242
AciI CCGC 1 cut(s) 240
AfaI GTAC 2 cut(s) 94, 453
AflIII ACRYGT 1 cut(s) 421
AgsI TTSAA 3 cut(s) 301, 565, 604
AhlI ACTAGT 1 cut(s) 268
AluBI AGCT 1 cut(s) 695
AluI AGCT 1 cut(s) 695
Alw26I GTCTC 2 cut(s) 96, 503
AlwNI CAGNNNCTG 1 cut(s) 131
Ama87I CYCGRG 1 cut(s) 461
ApeKI GCWGC 4 cut(s) 53, 125, 160, 695
AvaI CYCGRG 1 cut(s) 461
BanI GGYRCC 1 cut(s) 397
BauI CACGAG 2 cut(s) 324, 711
BbsI GAAGAC 1 cut(s) 225
BbvI GCAGC 4 cut(s) 65, 137, 172, 682
BccI CCATC 2 cut(s) 403, 571
BcgI CGANNNNNNTGC 2 cut(s) 42, 76
BclI TGATCA 1 cut(s) 280
BcoDI GTCTC 2 cut(s) 96, 503
BcuI ACTAGT 1 cut(s) 268
BfaI CTAG 2 cut(s) 75, 269
BfmI CTRYAG 1 cut(s) 96
BisI GCNGC 5 cut(s) 54, 126, 161, 240, 696
BlsI GCNGC 5 cut(s) 55, 127, 162, 241, 697
BmcAI AGTACT 1 cut(s) 94
BmeT110I CYCGRG 1 cut(s) 461
BmiI GGNNCC 1 cut(s) 399
BmrI ACTGGG 2 cut(s) 311, 640
BmsI GCATC 5 cut(s) 112, 154, 176, 430, 526
BmuI ACTGGG 2 cut(s) 311, 640
BpiI GAAGAC 1 cut(s) 225
BplI GAGNNNNNCTC 1 cut(s) 31
BpuEI CTTGAG 2 cut(s) 403, 559
BsaBI GATNNNNATC 1 cut(s) 581
BsaI GGTCTC 1 cut(s) 503
BsaJI CCNNGG 1 cut(s) 373
BsaWI WCCGGW 1 cut(s) 208
Bse118I RCCGGY 2 cut(s) 163, 473
Bse1I ACTGG 4 cut(s) 90, 200, 317, 635
Bse8I GATNNNNATC 1 cut(s) 581
BseDI CCNNGG 1 cut(s) 373
BseGI GGATG 3 cut(s) 445, 582, 616
BseJI GATNNNNATC 1 cut(s) 581
BseNI ACTGG 4 cut(s) 90, 200, 317, 635
BseXI GCAGC 4 cut(s) 65, 137, 172, 682
BseYI CCCAGC 1 cut(s) 331
BshNI GGYRCC 1 cut(s) 397
BsiHKCI CYCGRG 1 cut(s) 461
BsiSI CCGG 3 cut(s) 164, 209, 474
BslFI GGGAC 1 cut(s) 647
BsmAI GTCTC 2 cut(s) 96, 503
BsmFI GGGAC 1 cut(s) 647
Bso31I GGTCTC 1 cut(s) 503
BsoBI CYCGRG 1 cut(s) 461
Bsp1407I TGTACA 1 cut(s) 451
Bsp143I GATC 2 cut(s) 33, 280
BspACI CCGC 1 cut(s) 240
BspHI TCATGA 1 cut(s) 283
BspLI GGNNCC 1 cut(s) 399
BspMAI CTGCAG 1 cut(s) 100
BspT107I GGYRCC 1 cut(s) 397
BspTNI GGTCTC 1 cut(s) 503
BsrBI CCGCTC 1 cut(s) 242
BsrFI RCCGGY 2 cut(s) 163, 473
BsrGI TGTACA 1 cut(s) 451
BsrI ACTGG 4 cut(s) 90, 200, 317, 635
BssAI RCCGGY 2 cut(s) 163, 473
BssECI CCNNGG 1 cut(s) 373
BssMI GATC 2 cut(s) 33, 280
BssSI CACGAG 2 cut(s) 324, 711
BssT1I CCWWGG 1 cut(s) 373
Bst2BI CACGAG 2 cut(s) 324, 711
Bst4CI ACNGT 1 cut(s) 490
Bst6I CTCTTC 3 cut(s) 545, 589, 669
BstAPI GCANNNNNTGC 1 cut(s) 131
BstAUI TGTACA 1 cut(s) 451
BstC8I GCNNGC 4 cut(s) 51, 165, 389, 679
BstDEI CTNAG 1 cut(s) 365
BstF5I GGATG 3 cut(s) 445, 582, 616
BstKTI GATC 2 cut(s) 36, 283
BstMAI GTCTC 2 cut(s) 96, 503
BstMBI GATC 2 cut(s) 33, 280
BstMWI GCNNNNNNNGC 3 cut(s) 131, 397, 687
BstNSI RCATGY 2 cut(s) 53, 425
BstSFI CTRYAG 1 cut(s) 96
BstV1I GCAGC 4 cut(s) 65, 137, 172, 682
BstV2I GAAGAC 1 cut(s) 225
BtsCI GGATG 3 cut(s) 445, 582, 616
BtsI GCAGTG 1 cut(s) 129
BtsIMutI CAGTG 2 cut(s) 129, 324
Cac8I GCNNGC 4 cut(s) 51, 165, 389, 679
CaiI CAGNNNCTG 1 cut(s) 131
CciI TCATGA 1 cut(s) 283
Cfr10I RCCGGY 2 cut(s) 163, 473
Csp6I GTAC 2 cut(s) 93, 452
CviAII CATG 3 cut(s) 50, 284, 422
CviQI GTAC 2 cut(s) 93, 452
DdeI CTNAG 1 cut(s) 365
DpnI GATC 2 cut(s) 35, 282
DpnII GATC 2 cut(s) 33, 280
Eam1104I CTCTTC 3 cut(s) 545, 589, 669
EarI CTCTTC 3 cut(s) 545, 589, 669
Eco130I CCWWGG 1 cut(s) 373
Eco31I GGTCTC 1 cut(s) 503
Eco88I CYCGRG 1 cut(s) 461
EcoT14I CCWWGG 1 cut(s) 373
EcoT22I ATGCAT 1 cut(s) 51
ErhI CCWWGG 1 cut(s) 373
FaeI CATG 3 cut(s) 53, 287, 425
FaiI YATR 7 cut(s) 51, 206, 285, 293, 340, 423, 558
FalI AAGNNNNNCTT 2 cut(s) 641, 673
FaqI GGGAC 1 cut(s) 647
FatI CATG 3 cut(s) 49, 283, 421
FbaI TGATCA 1 cut(s) 280
Fnu4HI GCNGC 5 cut(s) 54, 126, 161, 240, 696
FokI GGATG 3 cut(s) 452, 589, 623
Fsp4HI GCNGC 5 cut(s) 54, 126, 161, 240, 696
FspBI CTAG 2 cut(s) 75, 269
GluI GCNGC 5 cut(s) 54, 126, 161, 240, 696
GsaI CCCAGC 1 cut(s) 335
HapII CCGG 3 cut(s) 164, 209, 474
Hin1II CATG 3 cut(s) 53, 287, 425
HincII GTYRAC 1 cut(s) 360
HindII GTYRAC 1 cut(s) 360
HinfI GANTC 5 cut(s) 59, 225, 361, 459, 497
HpaII CCGG 3 cut(s) 164, 209, 474
Hpy166II GTNNAC 2 cut(s) 360, 479
Hpy188I TCNGA 4 cut(s) 255, 280, 502, 669
Hpy188III TCNNGA 6 cut(s) 229, 284, 326, 415, 463, 586
Hpy8I GTNNAC 2 cut(s) 360, 479
Hpy99I CGWCG 1 cut(s) 550
HpyAV CCTTC 3 cut(s) 441, 583, 601
HpyCH4III ACNGT 1 cut(s) 490
HpyCH4IV ACGT 1 cut(s) 310
HpyCH4V TGCA 7 cut(s) 49, 53, 98, 125, 145, 160, 677
HpyF10VI GCNNNNNNNGC 3 cut(s) 131, 397, 687
HpyF3I CTNAG 1 cut(s) 365
HpySE526I ACGT 1 cut(s) 310
Hsp92II CATG 3 cut(s) 53, 287, 425
KroI GCCGGC 1 cut(s) 163
KroNI GCCGGC 1 cut(s) 165
Ksp22I TGATCA 1 cut(s) 280
Kzo9I GATC 2 cut(s) 33, 280
LmnI GCTCC 1 cut(s) 247
Lsp1109I GCAGC 4 cut(s) 65, 137, 172, 682
LweI GCATC 5 cut(s) 112, 154, 176, 430, 526
MaeI CTAG 2 cut(s) 75, 269
MaeII ACGT 1 cut(s) 310
MaeIII GTNAC 5 cut(s) 311, 326, 368, 484, 706
MalI GATC 2 cut(s) 35, 282
MbiI CCGCTC 1 cut(s) 242
MboI GATC 2 cut(s) 33, 280
MboII GAAGA 9 cut(s) 17, 230, 313, 316, 364, 562, 606, 616, 656
MluCI AATT 5 cut(s) 28, 521, 571, 618, 643
MlyI GAGTC 3 cut(s) 355, 453, 491
MnlI CCTC 4 cut(s) 25, 35, 76, 534
Mph1103I ATGCAT 1 cut(s) 51
MroNI GCCGGC 1 cut(s) 163
MseI TTAA 4 cut(s) 44, 137, 492, 524
MspI CCGG 3 cut(s) 164, 209, 474
MwoI GCNNNNNNNGC 3 cut(s) 131, 397, 687
NaeI GCCGGC 1 cut(s) 165
NdeII GATC 2 cut(s) 33, 280
NgoMIV GCCGGC 1 cut(s) 163
NlaIII CATG 3 cut(s) 53, 287, 425
NlaIV GGNNCC 1 cut(s) 399
NmuCI GTSAC 3 cut(s) 311, 326, 368
NsiI ATGCAT 1 cut(s) 51
NspI RCATGY 2 cut(s) 53, 425
PaeI GCATGC 1 cut(s) 53
PaeR7I CTCGAG 1 cut(s) 461
PagI TCATGA 1 cut(s) 283
PciI ACATGT 1 cut(s) 421
PdiI GCCGGC 1 cut(s) 165
PfeI GAWTC 2 cut(s) 59, 225
PkrI GCNGC 5 cut(s) 55, 127, 162, 241, 697
PleI GAGTC 3 cut(s) 355, 453, 491
PpsI GAGTC 3 cut(s) 355, 453, 491
PscI ACATGT 1 cut(s) 421
PspFI CCCAGC 1 cut(s) 331
PspN4I GGNNCC 1 cut(s) 399
PstI CTGCAG 1 cut(s) 100
PstNI CAGNNNCTG 1 cut(s) 131
RsaI GTAC 2 cut(s) 94, 453
RsaNI GTAC 2 cut(s) 93, 452
SaqAI TTAA 4 cut(s) 44, 137, 492, 524
SatI GCNGC 5 cut(s) 54, 126, 161, 240, 696
Sau3AI GATC 2 cut(s) 33, 280
ScaI AGTACT 1 cut(s) 94
SchI GAGTC 3 cut(s) 355, 453, 491
SetI ASST 4 cut(s) 313, 375, 545, 697
SfaNI GCATC 5 cut(s) 112, 154, 176, 430, 526
SfcI CTRYAG 1 cut(s) 96
Sfr274I CTCGAG 1 cut(s) 461
SlaI CTCGAG 1 cut(s) 461
SmlI CTYRAG 3 cut(s) 382, 461, 538
SmoI CTYRAG 3 cut(s) 382, 461, 538
SpeI ACTAGT 1 cut(s) 268
SphI GCATGC 1 cut(s) 53
Sse9I AATT 5 cut(s) 28, 521, 571, 618, 643
SsiI CCGC 1 cut(s) 240
SspMI CTAG 2 cut(s) 75, 269
StyI CCWWGG 1 cut(s) 373
TaaI ACNGT 1 cut(s) 490
TaiI ACGT 1 cut(s) 313
TaqI TCGA 5 cut(s) 36, 170, 228, 462, 548
TaqII GACCGA 1 cut(s) 527
TasI AATT 5 cut(s) 28, 521, 571, 618, 643
TatI WGTACW 2 cut(s) 92, 451
TauI GCSGC 1 cut(s) 242
TfiI GAWTC 2 cut(s) 59, 225
Tru1I TTAA 4 cut(s) 44, 137, 492, 524
Tru9I TTAA 4 cut(s) 44, 137, 492, 524
TscAI CASTG 2 cut(s) 136, 324
TseFI GTSAC 3 cut(s) 311, 326, 368
TseI GCWGC 4 cut(s) 53, 125, 160, 695
Tsp45I GTSAC 3 cut(s) 311, 326, 368
TspDTI ATGAA 3 cut(s) 630, 707, 717
TspRI CASTG 2 cut(s) 136, 324
XceI RCATGY 2 cut(s) 53, 425
XhoI CTCGAG 1 cut(s) 461
XspI CTAG 2 cut(s) 75, 269
ZrmI AGTACT 1 cut(s) 94
Zsp2I ATGCAT 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.