Prupe.2G178700_v2.0.a1

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0016654
Physical Location & Seq
Forward (+)
22282616 .. 22284518
1903 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G178700.1

Sequence Viewer

Length: 1434 bp
ATGGGGAAACCAAATGTTTTTAATAAGTTAGGAACATGGAGAGGAGGAGTTCGCAGCTCGATCTTTGTATTAAGTATCTTTGCTCTTCTCATCTCTCTTGGTACTTTGTCCAAATTCAACTCTCTCGGATCATTTCATGTCACTGATACTCTCTGCAATTATGTAAACCCTGAGAGCCAAAGCAGGCCAGACAGCCATGTGAGAAGAACTCTTGAAACTGTTATTCAGAAAATCCAACAAGAGCTCGACAAGTTCAGAGATTCAGAACCAGAGCCGTCTTCATCGAAACATGTACTGAAATACAATTCCTTTCTTGCTGATATTTTGGGGCATATTGAGTCAGTGCGGACATCTCTGTCACAGAATGAAGAGACTCATGAGCTGAACAGTGGTGCCAAAGACATCCATCCTCTCTTAAAACCGAAGCAGCAGTCCGATGAGCCTGCAGATTACTTTCTGATCGAGGAGATTCGAAAGTATGTCAAGATCAAGCCGAATAGATTAGGAAAGCAGAATTTCATGGGAGCCAATGGAACTTTCACCAGTATTGGCCATGCTTGCTTTGCTATGAAGAAAGAGCTTGAAGAATACATGGACTATGACATTGGTGAAATCTGCAATGATGATTGGAGGCTGGCTCAGAAGCTAATGGTTCATGGATGTGATCCACTTCCAAGGAGGAGGTGCTTTTCCAGGGCCCCAAAGCTATACAGCAAGCCATTTCCAATTCATGAGTCCATGTGGAAGCTCCCTGATAATCGAAATGTCCGGTGGAGCAACTACAGGTGCAAAAACTTTACTTGCTTGGCAAGCAACAAGACTCAAAAGGGGTTCTTCAAATGTGCAGATTGCTTCAATCTTCAAGACCATGAAAAGCCCAGATGGATCAAACCAGCATATCTAGACACCAATTCAAATGTGACTGCAGATTTCCTCATCCCTGAAGTGCTTAACATCAAGCCTGGAGAGATCAGAATTGGACTGGATTTTAGTGTAGGAACTGGGACTTTTGCAGCCAGGATGAGGGAATTCAATGTCACTATTGTCTCAGCAACTATCAATCTTGGGGCACCTTTCAATGAAATGATAGCTCTTCGTGGACTCGTCCCACTTTACTTGACGATCAATCAACGGGTGCCATTTTTCGATAACACTCTGGATTTGATCCACACAACAAGATTTCTAGATGGTTGGATTGATTTTGTGCTGCTGGACTTTGTTTTGTATGATTGGGACAGAGCTCTGAGGCCTGGGGGTTTCTTGTGGGTTGACAGCTTCTTCTGTTTGAAGGAGGATTTGAGTGATTATTTGGAAGCCCTTAGGATGCTGAGATATAAGAAGCACAAATGGGTTGTTGTTCCAAAGCTTGATAAAGATGATAAAGAGGTTTTCTTTTCTGCTCTATTAGAAAAGCCGGGTAGACCTTTCCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29

Protein Analysis

478

Amino Acids

55.1

Weight (kDa)

8.74

Isoelectric Point (pI)

40.33

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016654)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G01240
fragaria_vesca FvH4_7g15510
malus_domestica MD07G1130500.v1.1
prunus_persica Prupe.2G178700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0357841
rosa_laevigata RLG00000027992
rosa_multiflora Rmu_sc0002071.1_g000002 Rmu_sc0011109.1_g000006
rosa_roxburghii Rroxscaffold_4G00298460
rosa_rugosa Rorug01G0258900
rosa_samantha Rh1AG272900 Rh1BG240200 Rh1DG268500
rosa_wichuraiana Rw1G024360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 355
AccB1I GGYRCC 3 cut(s) 392, 1069, 1135
AccI GTMKAC 1 cut(s) 1420
AciI CCGC 1 cut(s) 346
AclWI GGATC 4 cut(s) 136, 659, 893, 1159
AcoI YGGCCR 1 cut(s) 550
AcsI RAATTY 3 cut(s) 113, 514, 1028
AcuI CTGAAG 1 cut(s) 963
AfaI GTAC 2 cut(s) 103, 294
AfiI CCNNNNNNNGG 1 cut(s) 1023
AflIII ACRYGT 1 cut(s) 289
AjnI CCWGG 4 cut(s) 692, 961, 1016, 1249
AjuI GAANNNNNNNTTGG 2 cut(s) 521, 553
Alw21I GWGCWC 2 cut(s) 246, 1243
Alw26I GTCTC 2 cut(s) 365, 1051
AlwI GGATC 4 cut(s) 136, 659, 893, 1159
AoxI GGCC 4 cut(s) 185, 550, 696, 1247
ApaI GGGCCC 1 cut(s) 700
ApeKI GCWGC 4 cut(s) 54, 427, 1013, 1207
ApoI RAATTY 3 cut(s) 113, 514, 1028
ArsI GACNNNNNNTTYG 2 cut(s) 416, 448
AspS9I GGNCC 2 cut(s) 696, 697
AsuC2I CCSGG 1 cut(s) 1416
AsuHPI GGTGA 2 cut(s) 532, 620
AsuII TTCGAA 1 cut(s) 472
AxyI CCTNAGG 1 cut(s) 1319
BaeGI GKGCMC 2 cut(s) 700, 1072
BalI TGGCCA 1 cut(s) 552
BanI GGYRCC 3 cut(s) 392, 1069, 1135
BanII GRGCYC 3 cut(s) 246, 700, 1243
BbsI GAAGAC 1 cut(s) 270
Bbv12I GWGCWC 2 cut(s) 246, 1243
BbvI GCAGC 4 cut(s) 66, 439, 1025, 1194
BccI CCATC 3 cut(s) 414, 876, 1181
BceAI ACGGC 1 cut(s) 259
BcgI CGANNNNNNTGC 2 cut(s) 425, 459
BciT130I CCWGG 4 cut(s) 694, 963, 1018, 1251
BcnI CCSGG 1 cut(s) 1416
BcoDI GTCTC 2 cut(s) 365, 1051
BfaI CTAG 2 cut(s) 902, 1184
BfmI CTRYAG 3 cut(s) 444, 781, 924
BisI GCNGC 4 cut(s) 55, 428, 1014, 1208
BlsI GCNGC 4 cut(s) 56, 429, 1015, 1209
Bme1390I CCNGG 5 cut(s) 694, 963, 1018, 1251, 1416
BmgT120I GGNCC 2 cut(s) 696, 697
BmiI GGNNCC 6 cut(s) 394, 526, 698, 699, 1071, 1137
BmrFI CCNGG 5 cut(s) 694, 963, 1018, 1251, 1416
BmrI ACTGGG 1 cut(s) 1011
BmsI GCATC 1 cut(s) 1314
BmuI ACTGGG 1 cut(s) 1011
BpiI GAAGAC 1 cut(s) 270
BplI GAGNNNNNCTC 4 cut(s) 193, 225, 622, 654
BpmI CTGGAG 1 cut(s) 984
Bpu14I TTCGAA 1 cut(s) 472
BpuMI CCSGG 1 cut(s) 1416
BsaJI CCNNGG 3 cut(s) 674, 693, 1250
BsaWI WCCGGW 2 cut(s) 768, 1428
Bsc4I CCNNNNNNNGG 1 cut(s) 1023
Bse1I ACTGG 3 cut(s) 543, 987, 1006
Bse21I CCTNAGG 1 cut(s) 1319
Bse3DI GCAATG 1 cut(s) 625
BseBI CCWGG 4 cut(s) 694, 963, 1018, 1251
BseDI CCNNGG 3 cut(s) 674, 693, 1250
BseGI GGATG 6 cut(s) 402, 406, 665, 936, 1026, 1329
BseLI CCNNNNNNNGG 1 cut(s) 1023
BseMI GCAATG 1 cut(s) 625
BseMII CTCAG 5 cut(s) 162, 653, 1062, 1235, 1319
BseNI ACTGG 3 cut(s) 543, 987, 1006
BseRI GAGGAG 4 cut(s) 57, 60, 479, 694
BseSI GKGCMC 2 cut(s) 700, 1072
BseXI GCAGC 4 cut(s) 66, 439, 1025, 1194
BsgI GTGCAG 1 cut(s) 864
BshFI GGCC 4 cut(s) 187, 552, 698, 1249
BshNI GGYRCC 3 cut(s) 392, 1069, 1135
BsiHKAI GWGCWC 2 cut(s) 246, 1243
BsiSI CCGG 3 cut(s) 769, 1415, 1429
BslFI GGGAC 3 cut(s) 1018, 1091, 1247
BslI CCNNNNNNNGG 1 cut(s) 1023
BsmAI GTCTC 2 cut(s) 365, 1051
BsmFI GGGAC 3 cut(s) 1018, 1091, 1247
BsnI GGCC 4 cut(s) 187, 552, 698, 1249
Bsp119I TTCGAA 1 cut(s) 472
Bsp120I GGGCCC 1 cut(s) 696
Bsp1286I GDGCHC 4 cut(s) 246, 700, 1072, 1243
Bsp143I GATC 9 cut(s) 60, 128, 459, 486, 664, 885, 969, 1122, 1164
BspACI CCGC 1 cut(s) 346
BspANI GGCC 4 cut(s) 187, 552, 698, 1249
BspCNI CTCAG 5 cut(s) 163, 652, 1061, 1236, 1320
BspHI TCATGA 2 cut(s) 376, 730
BspLI GGNNCC 6 cut(s) 394, 526, 698, 699, 1071, 1137
BspMAI CTGCAG 2 cut(s) 448, 928
BspPI GGATC 4 cut(s) 136, 659, 893, 1159
BspQI GCTCTTC 2 cut(s) 90, 1098
BspT104I TTCGAA 1 cut(s) 472
BspT107I GGYRCC 3 cut(s) 392, 1069, 1135
BsrDI GCAATG 1 cut(s) 625
BsrI ACTGG 3 cut(s) 543, 987, 1006
BssECI CCNNGG 3 cut(s) 674, 693, 1250
BssMI GATC 9 cut(s) 60, 128, 459, 486, 664, 885, 969, 1122, 1164
BssT1I CCWWGG 1 cut(s) 674
Bst2UI CCWGG 4 cut(s) 694, 963, 1018, 1251
Bst4CI ACNGT 2 cut(s) 220, 389
Bst6I CTCTTC 3 cut(s) 90, 363, 1098
BstBI TTCGAA 1 cut(s) 472
BstC8I GCNNGC 6 cut(s) 185, 444, 559, 636, 716, 811
BstDEI CTNAG 6 cut(s) 171, 639, 1048, 1244, 1319, 1328
BstF5I GGATG 6 cut(s) 402, 406, 665, 936, 1026, 1329
BstKTI GATC 9 cut(s) 63, 131, 462, 489, 667, 888, 972, 1125, 1167
BstMAI GTCTC 2 cut(s) 365, 1051
BstMBI GATC 9 cut(s) 60, 128, 459, 486, 664, 885, 969, 1122, 1164
BstMWI GCNNNNNNNGC 3 cut(s) 558, 563, 810
BstNI CCWGG 4 cut(s) 694, 963, 1018, 1251
BstNSI RCATGY 1 cut(s) 293
BstSCI CCNGG 5 cut(s) 692, 961, 1016, 1249, 1414
BstSFI CTRYAG 3 cut(s) 444, 781, 924
BstSLI GKGCMC 2 cut(s) 700, 1072
BstV1I GCAGC 4 cut(s) 66, 439, 1025, 1194
BstV2I GAAGAC 1 cut(s) 270
Bsu36I CCTNAGG 1 cut(s) 1319
BsuRI GGCC 4 cut(s) 187, 552, 698, 1249
BtsCI GGATG 6 cut(s) 402, 406, 665, 936, 1026, 1329
BtsIMutI CAGTG 3 cut(s) 141, 348, 394
Cac8I GCNNGC 6 cut(s) 185, 444, 559, 636, 716, 811
CciI TCATGA 2 cut(s) 376, 730
Cfr13I GGNCC 2 cut(s) 696, 697
Csp6I GTAC 2 cut(s) 102, 293
CspCI CAANNNNNGTGG 2 cut(s) 1098, 1133
CviQI GTAC 2 cut(s) 102, 293
DdeI CTNAG 6 cut(s) 171, 639, 1048, 1244, 1319, 1328
DpnI GATC 9 cut(s) 62, 130, 461, 488, 666, 887, 971, 1124, 1166
DpnII GATC 9 cut(s) 60, 128, 459, 486, 664, 885, 969, 1122, 1164
DrdI GACNNNNNNGTC 1 cut(s) 355
DseDI GACNNNNNNGTC 1 cut(s) 355
EaeI YGGCCR 1 cut(s) 550
Eam1104I CTCTTC 3 cut(s) 90, 363, 1098
EarI CTCTTC 3 cut(s) 90, 363, 1098
Ecl136II GAGCTC 2 cut(s) 244, 1241
Eco130I CCWWGG 1 cut(s) 674
Eco147I AGGCCT 1 cut(s) 1249
Eco24I GRGCYC 3 cut(s) 246, 700, 1243
Eco53kI GAGCTC 2 cut(s) 244, 1241
Eco57I CTGAAG 1 cut(s) 963
Eco81I CCTNAGG 1 cut(s) 1319
EcoICRI GAGCTC 2 cut(s) 244, 1241
EcoO109I RGGNCCY 2 cut(s) 696, 697
EcoRI GAATTC 1 cut(s) 1028
EcoRII CCWGG 4 cut(s) 692, 961, 1016, 1249
EcoT14I CCWWGG 1 cut(s) 674
EcoT38I GRGCYC 3 cut(s) 246, 700, 1243
ErhI CCWWGG 1 cut(s) 674
FalI AAGNNNNNCTT 2 cut(s) 818, 850
FaqI GGGAC 3 cut(s) 1018, 1091, 1247
FblI GTMKAC 1 cut(s) 1420
Fnu4HI GCNGC 4 cut(s) 55, 428, 1014, 1208
FokI GGATG 6 cut(s) 389, 393, 672, 923, 1033, 1336
FriOI GRGCYC 3 cut(s) 246, 700, 1243
Fsp4HI GCNGC 4 cut(s) 55, 428, 1014, 1208
FspBI CTAG 2 cut(s) 902, 1184
GluI GCNGC 4 cut(s) 55, 428, 1014, 1208
GsuI CTGGAG 1 cut(s) 984
HaeIII GGCC 4 cut(s) 187, 552, 698, 1249
HapII CCGG 3 cut(s) 769, 1415, 1429
HincII GTYRAC 1 cut(s) 1270
HindII GTYRAC 1 cut(s) 1270
HindIII AAGCTT 1 cut(s) 1364
HinfI GANTC 7 cut(s) 260, 338, 373, 469, 734, 820, 1101
HpaII CCGG 3 cut(s) 769, 1415, 1429
HphI GGTGA 2 cut(s) 532, 620
Hpy166II GTNNAC 4 cut(s) 166, 1100, 1270, 1421
Hpy188I TCNGA 9 cut(s) 128, 228, 257, 265, 436, 459, 642, 974, 1245
Hpy188III TCNNGA 8 cut(s) 212, 377, 484, 731, 863, 902, 1157, 1184
Hpy8I GTNNAC 4 cut(s) 166, 1100, 1270, 1421
HpyAV CCTTC 1 cut(s) 1282
HpyCH4III ACNGT 2 cut(s) 220, 389
HpyCH4V TGCA 7 cut(s) 156, 446, 618, 789, 845, 926, 1013
HpyF10VI GCNNNNNNNGC 3 cut(s) 558, 563, 810
HpyF3I CTNAG 6 cut(s) 171, 639, 1048, 1244, 1319, 1328
Kzo9I GATC 9 cut(s) 60, 128, 459, 486, 664, 885, 969, 1122, 1164
LguI GCTCTTC 2 cut(s) 90, 1098
LmnI GCTCC 3 cut(s) 524, 753, 774
Lsp1109I GCAGC 4 cut(s) 66, 439, 1025, 1194
LweI GCATC 1 cut(s) 1314
MaeI CTAG 2 cut(s) 902, 1184
MaeIII GTNAC 4 cut(s) 139, 357, 919, 1036
MalI GATC 9 cut(s) 62, 130, 461, 488, 666, 887, 971, 1124, 1166
MboI GATC 9 cut(s) 60, 128, 459, 486, 664, 885, 969, 1122, 1164
MhlI GDGCHC 4 cut(s) 246, 700, 1072, 1243
MlsI TGGCCA 1 cut(s) 552
MluCI AATT 8 cut(s) 113, 157, 304, 514, 726, 910, 975, 1028
MluNI TGGCCA 1 cut(s) 552
MlyI GAGTC 5 cut(s) 347, 367, 743, 814, 1095
MmeI TCCRAC 2 cut(s) 259, 1172
Mox20I TGGCCA 1 cut(s) 552
MscI TGGCCA 1 cut(s) 552
MseI TTAA 4 cut(s) 21, 71, 416, 951
MslI CAYNNNNRTG 1 cut(s) 660
Msp20I TGGCCA 1 cut(s) 552
MspI CCGG 3 cut(s) 769, 1415, 1429
MspR9I CCNGG 5 cut(s) 694, 963, 1018, 1251, 1416
MvaI CCWGG 4 cut(s) 694, 963, 1018, 1251
MwoI GCNNNNNNNGC 3 cut(s) 558, 563, 810
NciI CCSGG 1 cut(s) 1416
NdeII GATC 9 cut(s) 60, 128, 459, 486, 664, 885, 969, 1122, 1164
NlaIV GGNNCC 6 cut(s) 394, 526, 698, 699, 1071, 1137
NmuCI GTSAC 4 cut(s) 139, 357, 919, 1036
NspI RCATGY 1 cut(s) 293
NspV TTCGAA 1 cut(s) 472
PagI TCATGA 2 cut(s) 376, 730
PceI AGGCCT 1 cut(s) 1249
PciI ACATGT 1 cut(s) 289
PciSI GCTCTTC 2 cut(s) 90, 1098
PfeI GAWTC 2 cut(s) 260, 469
PkrI GCNGC 4 cut(s) 56, 429, 1015, 1209
PleI GAGTC 5 cut(s) 346, 367, 742, 814, 1095
PpsI GAGTC 5 cut(s) 346, 367, 742, 814, 1095
PscI ACATGT 1 cut(s) 289
Psp124BI GAGCTC 2 cut(s) 246, 1243
Psp6I CCWGG 4 cut(s) 692, 961, 1016, 1249
PspGI CCWGG 4 cut(s) 692, 961, 1016, 1249
PspN4I GGNNCC 6 cut(s) 394, 526, 698, 699, 1071, 1137
PspOMI GGGCCC 1 cut(s) 696
PspPI GGNCC 2 cut(s) 696, 697
PstI CTGCAG 2 cut(s) 448, 928
RsaI GTAC 2 cut(s) 103, 294
RsaNI GTAC 2 cut(s) 102, 293
RseI CAYNNNNRTG 1 cut(s) 660
SacI GAGCTC 2 cut(s) 246, 1243
SapI GCTCTTC 2 cut(s) 90, 1098
SaqAI TTAA 4 cut(s) 21, 71, 416, 951
SatI GCNGC 4 cut(s) 55, 428, 1014, 1208
Sau3AI GATC 9 cut(s) 60, 128, 459, 486, 664, 885, 969, 1122, 1164
Sau96I GGNCC 2 cut(s) 696, 697
SchI GAGTC 5 cut(s) 347, 367, 743, 814, 1095
ScrFI CCNGG 5 cut(s) 694, 963, 1018, 1251, 1416
SduI GDGCHC 4 cut(s) 246, 700, 1072, 1243
SfaNI GCATC 1 cut(s) 1314
SfcI CTRYAG 3 cut(s) 444, 781, 924
SfuI TTCGAA 1 cut(s) 472
SmiMI CAYNNNNRTG 1 cut(s) 660
Sse9I AATT 8 cut(s) 113, 157, 304, 514, 726, 910, 975, 1028
SseBI AGGCCT 1 cut(s) 1249
SsiI CCGC 1 cut(s) 346
SspMI CTAG 2 cut(s) 902, 1184
SstI GAGCTC 2 cut(s) 246, 1243
StuI AGGCCT 1 cut(s) 1249
StyD4I CCNGG 5 cut(s) 692, 961, 1016, 1249, 1414
StyI CCWWGG 1 cut(s) 674
TaaI ACNGT 2 cut(s) 220, 389
TaqI TCGA 7 cut(s) 59, 246, 284, 462, 472, 760, 1146
TasI AATT 8 cut(s) 113, 157, 304, 514, 726, 910, 975, 1028
TatI WGTACW 1 cut(s) 292
TfiI GAWTC 2 cut(s) 260, 469
Tru1I TTAA 4 cut(s) 21, 71, 416, 951
Tru9I TTAA 4 cut(s) 21, 71, 416, 951
TscAI CASTG 3 cut(s) 148, 348, 394
TseFI GTSAC 4 cut(s) 139, 357, 919, 1036
TseI GCWGC 4 cut(s) 54, 427, 1013, 1207
Tsp45I GTSAC 4 cut(s) 139, 357, 919, 1036
TspDTI ATGAA 9 cut(s) 125, 270, 381, 508, 584, 644, 719, 885, 1095
TspRI CASTG 3 cut(s) 148, 348, 394
XapI RAATTY 3 cut(s) 113, 514, 1028
XbaI TCTAGA 2 cut(s) 901, 1183
XceI RCATGY 1 cut(s) 293
XmiI GTMKAC 1 cut(s) 1420
XspI CTAG 2 cut(s) 902, 1184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.