Rh1AG272900

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0016654
Physical Location & Seq
Forward (+)
50076663 .. 50078102
1440 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG272900.1

Sequence Viewer

Length: 1440 bp
ATGAGGAAACCGAGTGTTGTTATGAAGTTGGGAACATGGAGAGGAGTTCAGAGGTCAATTTTTATATTGGGTAGTTTTGCTCTTCTCATCTCAATTGTTACCTTATCATCCAAATTCTACTCTCTCAAATCATTTCATGTTACCGATACCTTCTGCAATTATGTAAATACTGATAGTCAAAGCAGGATTGACAGTCATGTAAAAGTCAATCTCGAAACTGTGATTCAGAAACTCCAACAGGAACTTAAAACGTTCAGAGATTCAACAGCAGATTCGTCGTCATCTGTGCATTTGTTAAAGCACAGCACGTTTCTTGCTGATATTTTGGGGCTGATCGAGTCGGTGCATGAATCTCTGGTGCAGAATGATGAGACGACTCAGAAGTTAAGCAATGATGTCAAAGACATCCATCCTCTGCTAAAACCGAAGCAGCACTCTGATGAGCCTGCGGAGTATTTTCTAATAGAAGAGATTCGGAAGTATGTCAAGATCAAGCCGAACAGATTGGGAAAGCAGAATTTCATGGGAGCTAATGGGACTTTCACTAGCATTGGCCATGCTTGCTTTGCTATGAAGACAGAACTTGAAGAATACATGAACTACGACATTGGCGATATCTGCAATGACGATTGGAAGCTAGCTCAGAAGCTCATGGTTCATGGATGCGATCCACTTCCGAGGAGGAGGTGCTTCGCTCTAGCCCCAAAGATATATAGCAAGCCATTCCCAATCCATGAGTCCATATGGAAGCTCCCAGACAATAGAAATGTCCGGTGGAGTCAGTATCGGTGCAAGAACTTTACTTGCCTCGCAAGTAATTCCGCTCCCAAGGGGTTCTTCAAATGTGCAGATTGCTTCAATCTGTCACACCACGAAATGCCTAGATGGATGCAACAAGTAAGTCTAGACTCAAAATTGAATCTGACTGCAGATTTTCTTATCCCTGAAGTGCTTCAAATCAAGCCGGGAGAGATCAGAATCGGACTGGATTTTAGCGTAGGGACTGGGACTTTTGCAGCTAGGATGAGGGAGTTCAATGTCACCATGGTCTCGGCAACTATCAATCTCGGGGCACCTTTTAATGAAATGATAGCTCTTCGTGGACTCGTCCCTCTTTACTTGACAATCAATCAACGGCTGCCATTCTTCGACAACACTCTGGATTTGATTCACACCACAAGATTTCTGGATGGCTGGATTGATTTTGTGCTGCTAGACTTTGTCTTGTATGATTGGGATAGAGTTTTGAGGCCTGGGGGTTTGCTGTGGATTGATAGCTTCTTCTGTGTGAAGGAAGATTTGAATGCTTATTTGGAGTCCCTTAAGATGCTTAGGTACAAGAAGCACAAATGGGTTGTTGTTCCAAAGATTGATAAAGATGATGATAGAGAGGTTTTCTTTTCTGCTGTTCTAGAAAAACCGCCCAGACCATTCCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29 zf-RING_2

Protein Analysis

479

Amino Acids

55.31

Weight (kDa)

8.64

Isoelectric Point (pI)

37.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 319 - 442 9.9e-06 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016654)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G01240
fragaria_vesca FvH4_7g15510
malus_domestica MD07G1130500.v1.1
prunus_persica Prupe.2G178700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0357841
rosa_laevigata RLG00000027992
rosa_multiflora Rmu_sc0002071.1_g000002 Rmu_sc0011109.1_g000006
rosa_roxburghii Rroxscaffold_4G00298460
rosa_rugosa Rorug01G0258900
rosa_samantha Rh1AG272900 Rh1BG240200 Rh1DG268500
rosa_wichuraiana Rw1G024360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1072
AccBSI CCGCTC 1 cut(s) 824
AciI CCGC 3 cut(s) 449, 822, 1421
AclI AACGTT 1 cut(s) 251
AclWI GGATC 1 cut(s) 662
AcoI YGGCCR 1 cut(s) 553
AcsI RAATTY 2 cut(s) 113, 517
AcuI CTGAAG 1 cut(s) 966
AfaI GTAC 1 cut(s) 1337
AflII CTTAAG 1 cut(s) 1322
AgsI TTSAA 8 cut(s) 264, 587, 841, 859, 919, 956, 1036, 1303
AjnI CCWGG 1 cut(s) 1252
AjuI GAANNNNNNNTTGG 4 cut(s) 821, 853, 1295, 1327
AluBI AGCT 8 cut(s) 530, 637, 641, 649, 751, 1019, 1094, 1278
AluI AGCT 8 cut(s) 530, 637, 641, 649, 751, 1019, 1094, 1278
Alw26I GTCTC 2 cut(s) 365, 1054
AlwI GGATC 1 cut(s) 662
Ama87I CYCGRG 1 cut(s) 1067
AoxI GGCC 2 cut(s) 553, 1250
ApeKI GCWGC 4 cut(s) 430, 1016, 1138, 1210
ApoI RAATTY 2 cut(s) 113, 517
Asp700I GAANNNNTTC 2 cut(s) 471, 951
AsuC2I CCSGG 1 cut(s) 966
AsuHPI GGTGA 1 cut(s) 1033
AsuNHI GCTAGC 1 cut(s) 637
AvaI CYCGRG 1 cut(s) 1067
BaeGI GKGCMC 1 cut(s) 1075
BalI TGGCCA 1 cut(s) 555
BanI GGYRCC 1 cut(s) 1072
BbsI GAAGAC 1 cut(s) 581
BbvI GCAGC 4 cut(s) 442, 1028, 1125, 1197
BccI CCATC 3 cut(s) 417, 879, 1184
BceAI ACGGC 1 cut(s) 1151
BcgI CGANNNNNNTGC 2 cut(s) 258, 292
BciT130I CCWGG 1 cut(s) 1254
BcnI CCSGG 1 cut(s) 966
BcoDI GTCTC 2 cut(s) 365, 1054
BfaI CTAG 8 cut(s) 546, 638, 698, 882, 905, 1020, 1214, 1412
BfmI CTRYAG 1 cut(s) 927
BfrI CTTAAG 1 cut(s) 1322
BisI GCNGC 4 cut(s) 431, 1017, 1139, 1211
BlsI GCNGC 4 cut(s) 432, 1018, 1140, 1212
Bme1390I CCNGG 2 cut(s) 966, 1254
BmeT110I CYCGRG 1 cut(s) 1067
BmiI GGNNCC 1 cut(s) 1074
BmrFI CCNGG 2 cut(s) 966, 1254
BmrI ACTGGG 1 cut(s) 1014
BmsI GCATC 3 cut(s) 653, 879, 1317
BmtI GCTAGC 1 cut(s) 641
BmuI ACTGGG 1 cut(s) 1014
BpiI GAAGAC 1 cut(s) 581
Bpu10I CCTNAGC 1 cut(s) 1331
BpuMI CCSGG 1 cut(s) 966
BsaBI GATNNNNATC 1 cut(s) 977
BsaI GGTCTC 1 cut(s) 1054
BsaJI CCNNGG 4 cut(s) 677, 828, 1044, 1253
BsaWI WCCGGW 2 cut(s) 771, 1434
Bse1I ACTGG 2 cut(s) 990, 1009
Bse3DI GCAATG 2 cut(s) 397, 628
Bse8I GATNNNNATC 1 cut(s) 977
BseBI CCWGG 1 cut(s) 1254
BseDI CCNNGG 4 cut(s) 677, 828, 1044, 1253
BseGI GGATG 7 cut(s) 107, 405, 409, 668, 894, 1029, 1195
BseJI GATNNNNATC 1 cut(s) 977
BseMI GCAATG 2 cut(s) 397, 628
BseMII CTCAG 2 cut(s) 392, 656
BseNI ACTGG 2 cut(s) 990, 1009
BseRI GAGGAG 3 cut(s) 57, 694, 697
BseSI GKGCMC 1 cut(s) 1075
BseXI GCAGC 4 cut(s) 442, 1028, 1125, 1197
BsgI GTGCAG 2 cut(s) 380, 867
BshFI GGCC 2 cut(s) 555, 1252
BshNI GGYRCC 1 cut(s) 1072
BsiHKCI CYCGRG 1 cut(s) 1067
BsiSI CCGG 3 cut(s) 772, 965, 1435
BslFI GGGAC 5 cut(s) 550, 1015, 1021, 1094, 1303
BsmAI GTCTC 2 cut(s) 365, 1054
BsmBI CGTCTC 1 cut(s) 365
BsmFI GGGAC 5 cut(s) 550, 1015, 1021, 1094, 1303
BsmI GAATGC 1 cut(s) 1309
BsnI GGCC 2 cut(s) 555, 1252
Bso31I GGTCTC 1 cut(s) 1054
BsoBI CYCGRG 1 cut(s) 1067
Bsp1286I GDGCHC 1 cut(s) 1075
Bsp143I GATC 4 cut(s) 333, 489, 667, 972
Bsp19I CCATGG 1 cut(s) 1044
BspACI CCGC 3 cut(s) 449, 822, 1421
BspANI GGCC 2 cut(s) 555, 1252
BspCNI CTCAG 2 cut(s) 391, 655
BspLI GGNNCC 1 cut(s) 1074
BspMAI CTGCAG 1 cut(s) 931
BspOI GCTAGC 1 cut(s) 641
BspPI GGATC 1 cut(s) 662
BspQI GCTCTTC 2 cut(s) 87, 1101
BspT107I GGYRCC 1 cut(s) 1072
BspTI CTTAAG 1 cut(s) 1322
BspTNI GGTCTC 1 cut(s) 1054
BsrBI CCGCTC 1 cut(s) 824
BsrDI GCAATG 2 cut(s) 397, 628
BsrI ACTGG 2 cut(s) 990, 1009
BssECI CCNNGG 4 cut(s) 677, 828, 1044, 1253
BssMI GATC 4 cut(s) 333, 489, 667, 972
BssT1I CCWWGG 2 cut(s) 828, 1044
Bst2UI CCWGG 1 cut(s) 1254
Bst4CI ACNGT 2 cut(s) 194, 220
Bst6I CTCTTC 3 cut(s) 87, 462, 1101
BstAFI CTTAAG 1 cut(s) 1322
BstC8I GCNNGC 4 cut(s) 447, 562, 639, 719
BstDEI CTNAG 3 cut(s) 378, 642, 1331
BstDSI CCRYGG 1 cut(s) 1044
BstF5I GGATG 7 cut(s) 107, 405, 409, 668, 894, 1029, 1195
BstKTI GATC 4 cut(s) 336, 492, 670, 975
BstMAI GTCTC 2 cut(s) 365, 1054
BstMBI GATC 4 cut(s) 333, 489, 667, 972
BstMWI GCNNNNNNNGC 3 cut(s) 561, 566, 618
BstNI CCWGG 1 cut(s) 1254
BstSCI CCNGG 2 cut(s) 964, 1252
BstSFI CTRYAG 1 cut(s) 927
BstSLI GKGCMC 1 cut(s) 1075
BstV1I GCAGC 4 cut(s) 442, 1028, 1125, 1197
BstV2I GAAGAC 1 cut(s) 581
BsuRI GGCC 2 cut(s) 555, 1252
BtgI CCRYGG 1 cut(s) 1044
BtsCI GGATG 7 cut(s) 107, 405, 409, 668, 894, 1029, 1195
Cac8I GCNNGC 4 cut(s) 447, 562, 639, 719
Csp6I GTAC 1 cut(s) 1336
CviQI GTAC 1 cut(s) 1336
DdeI CTNAG 3 cut(s) 378, 642, 1331
DpnI GATC 4 cut(s) 335, 491, 669, 974
DpnII GATC 4 cut(s) 333, 489, 667, 972
EaeI YGGCCR 1 cut(s) 553
Eam1104I CTCTTC 3 cut(s) 87, 462, 1101
EarI CTCTTC 3 cut(s) 87, 462, 1101
Eco130I CCWWGG 2 cut(s) 828, 1044
Eco147I AGGCCT 1 cut(s) 1252
Eco31I GGTCTC 1 cut(s) 1054
Eco32I GATATC 1 cut(s) 616
Eco57I CTGAAG 1 cut(s) 966
Eco88I CYCGRG 1 cut(s) 1067
EcoRII CCWGG 1 cut(s) 1252
EcoRV GATATC 1 cut(s) 616
EcoT14I CCWWGG 2 cut(s) 828, 1044
ErhI CCWWGG 2 cut(s) 828, 1044
Esp3I CGTCTC 1 cut(s) 365
FalI AAGNNNNNCTT 2 cut(s) 821, 853
FaqI GGGAC 5 cut(s) 550, 1015, 1021, 1094, 1303
FauNDI CATATG 1 cut(s) 743
Fnu4HI GCNGC 4 cut(s) 431, 1017, 1139, 1211
FokI GGATG 7 cut(s) 94, 392, 396, 675, 901, 1036, 1202
Fsp4HI GCNGC 4 cut(s) 431, 1017, 1139, 1211
FspBI CTAG 8 cut(s) 546, 638, 698, 882, 905, 1020, 1214, 1412
GluI GCNGC 4 cut(s) 431, 1017, 1139, 1211
HaeIII GGCC 2 cut(s) 555, 1252
HapII CCGG 3 cut(s) 772, 965, 1435
HpaII CCGG 3 cut(s) 772, 965, 1435
HphI GGTGA 1 cut(s) 1033
Hpy166II GTNNAC 1 cut(s) 1103
Hpy188III TCNNGA 6 cut(s) 212, 487, 905, 1160, 1187, 1412
Hpy8I GTNNAC 1 cut(s) 1103
Hpy99I CGWCG 1 cut(s) 280
HpyAV CCTTC 2 cut(s) 160, 1285
HpyCH4III ACNGT 2 cut(s) 194, 220
HpyCH4IV ACGT 2 cut(s) 251, 308
HpyF10VI GCNNNNNNNGC 3 cut(s) 561, 566, 618
HpyF3I CTNAG 3 cut(s) 378, 642, 1331
HpySE526I ACGT 2 cut(s) 251, 308
Kzo9I GATC 4 cut(s) 333, 489, 667, 972
LguI GCTCTTC 2 cut(s) 87, 1101
LmnI GCTCC 3 cut(s) 527, 756, 829
Lsp1109I GCAGC 4 cut(s) 442, 1028, 1125, 1197
LweI GCATC 3 cut(s) 653, 879, 1317
MaeI CTAG 8 cut(s) 546, 638, 698, 882, 905, 1020, 1214, 1412
MaeII ACGT 2 cut(s) 251, 308
MaeIII GTNAC 4 cut(s) 97, 139, 864, 1039
MalI GATC 4 cut(s) 335, 491, 669, 974
MbiI CCGCTC 1 cut(s) 824
MboI GATC 4 cut(s) 333, 489, 667, 972
MboII GAAGA 9 cut(s) 74, 479, 586, 599, 829, 1088, 1138, 1273, 1307
MfeI CAATTG 1 cut(s) 93
MhlI GDGCHC 1 cut(s) 1075
MlsI TGGCCA 1 cut(s) 555
MluCI AATT 7 cut(s) 57, 93, 113, 157, 517, 817, 914
MluNI TGGCCA 1 cut(s) 555
MlyI GAGTC 7 cut(s) 347, 370, 746, 787, 902, 1098, 1325
MmeI TCCRAC 1 cut(s) 259
Mox20I TGGCCA 1 cut(s) 555
MroXI GAANNNNTTC 2 cut(s) 471, 951
MscI TGGCCA 1 cut(s) 555
MseI TTAA 5 cut(s) 246, 296, 386, 1080, 1323
MslI CAYNNNNRTG 1 cut(s) 438
Msp20I TGGCCA 1 cut(s) 555
MspCI CTTAAG 1 cut(s) 1322
MspI CCGG 3 cut(s) 772, 965, 1435
MspR9I CCNGG 2 cut(s) 966, 1254
MunI CAATTG 1 cut(s) 93
Mva1269I GAATGC 1 cut(s) 1309
MvaI CCWGG 1 cut(s) 1254
MwoI GCNNNNNNNGC 3 cut(s) 561, 566, 618
NciI CCSGG 1 cut(s) 966
NcoI CCATGG 1 cut(s) 1044
NdeI CATATG 1 cut(s) 743
NdeII GATC 4 cut(s) 333, 489, 667, 972
NheI GCTAGC 1 cut(s) 637
NlaIV GGNNCC 1 cut(s) 1074
NmeAIII GCCGAG 1 cut(s) 1031
NmuCI GTSAC 2 cut(s) 864, 1039
PceI AGGCCT 1 cut(s) 1252
PciSI GCTCTTC 2 cut(s) 87, 1101
PcsI WCGNNNNNNNCGW 1 cut(s) 609
PctI GAATGC 1 cut(s) 1309
PdmI GAANNNNTTC 2 cut(s) 471, 951
PfeI GAWTC 8 cut(s) 223, 260, 272, 350, 472, 919, 978, 1168
PflFI GACNNNGTC 1 cut(s) 1220
PkrI GCNGC 4 cut(s) 432, 1018, 1140, 1212
PleI GAGTC 7 cut(s) 346, 370, 745, 786, 902, 1098, 1324
PpsI GAGTC 7 cut(s) 346, 370, 745, 786, 902, 1098, 1324
Psp1406I AACGTT 1 cut(s) 251
Psp6I CCWGG 1 cut(s) 1252
PspGI CCWGG 1 cut(s) 1252
PspN4I GGNNCC 1 cut(s) 1074
PstI CTGCAG 1 cut(s) 931
PsyI GACNNNGTC 1 cut(s) 1220
RsaI GTAC 1 cut(s) 1337
RsaNI GTAC 1 cut(s) 1336
RseI CAYNNNNRTG 1 cut(s) 438
SapI GCTCTTC 2 cut(s) 87, 1101
SaqAI TTAA 5 cut(s) 246, 296, 386, 1080, 1323
SatI GCNGC 4 cut(s) 431, 1017, 1139, 1211
Sau3AI GATC 4 cut(s) 333, 489, 667, 972
SchI GAGTC 7 cut(s) 347, 370, 746, 787, 902, 1098, 1325
ScrFI CCNGG 2 cut(s) 966, 1254
SduI GDGCHC 1 cut(s) 1075
SfaNI GCATC 3 cut(s) 653, 879, 1317
SfcI CTRYAG 1 cut(s) 927
SmiMI CAYNNNNRTG 1 cut(s) 438
SmlI CTYRAG 1 cut(s) 1322
SmoI CTYRAG 1 cut(s) 1322
Sse9I AATT 7 cut(s) 57, 93, 113, 157, 517, 817, 914
SseBI AGGCCT 1 cut(s) 1252
SsiI CCGC 3 cut(s) 449, 822, 1421
SspMI CTAG 8 cut(s) 546, 638, 698, 882, 905, 1020, 1214, 1412
StuI AGGCCT 1 cut(s) 1252
StyD4I CCNGG 2 cut(s) 964, 1252
StyI CCWWGG 2 cut(s) 828, 1044
TaaI ACNGT 2 cut(s) 194, 220
TaiI ACGT 2 cut(s) 254, 311
TaqI TCGA 3 cut(s) 213, 336, 1149
TasI AATT 7 cut(s) 57, 93, 113, 157, 517, 817, 914
TfiI GAWTC 8 cut(s) 223, 260, 272, 350, 472, 919, 978, 1168
Tru1I TTAA 5 cut(s) 246, 296, 386, 1080, 1323
Tru9I TTAA 5 cut(s) 246, 296, 386, 1080, 1323
TseFI GTSAC 2 cut(s) 864, 1039
TseI GCWGC 4 cut(s) 430, 1016, 1138, 1210
Tsp45I GTSAC 2 cut(s) 864, 1039
TspDTI ATGAA 8 cut(s) 38, 125, 363, 511, 587, 611, 647, 1098
Tth111I GACNNNGTC 1 cut(s) 1220
Vha464I CTTAAG 1 cut(s) 1322
XapI RAATTY 2 cut(s) 113, 517
XbaI TCTAGA 2 cut(s) 904, 1411
XcmI CCANNNNNNNNNTGG 1 cut(s) 1183
XmnI GAANNNNTTC 2 cut(s) 471, 951
XspI CTAG 8 cut(s) 546, 638, 698, 882, 905, 1020, 1214, 1412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.