Rw1G024360

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0016654
Physical Location & Seq
Forward (+)
50558800 .. 50560364
1565 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G024360.1

Sequence Viewer

Length: 1440 bp
ATGAGGAAACCAAGTGTTGTTATGAAGTTGGGAACATGGAGAGGAGTTCAGAGGTCAATTTTTATATTGGGTAGTTTTGCTCTTCTCATCTCAATTGTTACCTTATCATCCAAATTCTACTCTCTCAAATCATTTCATGTTACTGATACCTTCTGCAATTATGTAAATACTGATAGTCAAAGCAGGATTGACAGTCATGTAAAAGTCACTCTCGAAACTGTGATTCAGAAACTCCAACAGGAACTTAACACGTTCAGAGATTCAACAACAGATACGTCGTCATCTGCGCATTTGTTAAAGCACAGTACGTTTCTTGCTGATATTTTGGGGCTGATCGAGTCGGTGCACGAATCTCTGGCGCAGAATGATGAGACGCCTCAGAAGTTAAGCAATGATGTCAAAGACATCCATCCTCTGCTAAAACCGAAGCAGCACTCTGATGAGCCTGCGGAGTATTTTCTAATAGAAGAGATTCGGAAGTATGTCAAGATCAAGCCGAACAGATTGGGAAAGCAGAATTTCATGGGAGCTAATGGGACTTTCACTAGCATTGGCCATGCTTGCTTTGCTATGAAGACAGAACTTGAAGAATACATGAACTACGACATTGGCGATATCTGCAATGACGATTGGAAGCTAGCTCAGAAGCTCATGGTTCATGGATGCGATCCACTTCCGAGGAGGAGGTGCTTCGCTCTAGCCCCAAAGATATATAGCAAGCCATTCCCAATACATGAGTCCATGTGGAAGCTCCCAGACAATAGAAATGTCCGGTGGAGTCAGTATCGGTGCAAGAACTTTACTTGCCTTGCAAGTAATTCCGCTCCCAAGGGGTTCTTCAAATGTGCTGATTGCTTCAATTTGTCACACCACGAAATGCCTAGATGGGTGCAACAAGTAAGTCTAGACTCAAAATTGAATCTGACTGCAGATTTTCTTATCCCCGAAGTGCTTCAAATCAAGCCGCGTGAGATCAGAATCGGACTGGATTTTAGCGTAGGGACTGGGACTTTTGCAGCTAGGATGAGGGAGTTCAATGTCACCATTGTCTCGGCAACTATCAATCTCGGGGCACCTTTTAATGAAATGATAGCTCTTCGTGGACTCGTCCCTCTTTACTTGACAATCAATCAACGGCTGCCATTCTTCGACAACACTCTGGATTTGATTCACACCACAAGATTTCTGGATGGCTGGATTGATTTTGTGCTGCTAGACTTTGTCTTGTATGATTGGGATAGAGTTTTGAGGCCTGGGGGTTTGCTGTGGATTGATAGCTTCTTCTGTGTGAAAGAAGATTTGAATGCTTATTTGGAGTCCCTTAAGATGCTGAGGTACAAGAAGCACAAATGGGTTGTTGTTCCAAAGATTGATAAAGATGATGATAGAGAGGTTTTCTTTTCTGCTGTTCTAGAAAAGCCGCCTAGACCATTCCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29 zf-RING_2

Protein Analysis

479

Amino Acids

55.34

Weight (kDa)

8.65

Isoelectric Point (pI)

36.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 319 - 442 6.1e-06 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016654)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G01240
fragaria_vesca FvH4_7g15510
malus_domestica MD07G1130500.v1.1
prunus_persica Prupe.2G178700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0357841
rosa_laevigata RLG00000027992
rosa_multiflora Rmu_sc0002071.1_g000002 Rmu_sc0011109.1_g000006
rosa_roxburghii Rroxscaffold_4G00298460
rosa_rugosa Rorug01G0258900
rosa_samantha Rh1AG272900 Rh1BG240200 Rh1DG268500
rosa_wichuraiana Rw1G024360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 288
AccB1I GGYRCC 1 cut(s) 1072
AccBSI CCGCTC 1 cut(s) 824
AccII CGCG 1 cut(s) 967
AciI CCGC 4 cut(s) 449, 822, 965, 1421
AclWI GGATC 1 cut(s) 662
AcoI YGGCCR 1 cut(s) 553
AcsI RAATTY 2 cut(s) 113, 517
AcyI GRCGYC 1 cut(s) 374
AfaI GTAC 2 cut(s) 307, 1337
AflII CTTAAG 1 cut(s) 1322
AflIII ACRYGT 1 cut(s) 249
AgsI TTSAA 8 cut(s) 264, 587, 841, 859, 919, 956, 1036, 1303
AjnI CCWGG 1 cut(s) 1252
AjuI GAANNNNNNNTTGG 4 cut(s) 821, 853, 1295, 1327
AluBI AGCT 8 cut(s) 530, 637, 641, 649, 751, 1019, 1094, 1278
AluI AGCT 8 cut(s) 530, 637, 641, 649, 751, 1019, 1094, 1278
Alw21I GWGCWC 1 cut(s) 348
Alw26I GTCTC 2 cut(s) 365, 1054
Alw44I GTGCAC 1 cut(s) 344
AlwI GGATC 1 cut(s) 662
Ama87I CYCGRG 1 cut(s) 1067
AoxI GGCC 2 cut(s) 553, 1250
ApaLI GTGCAC 1 cut(s) 344
ApeKI GCWGC 4 cut(s) 430, 1016, 1138, 1210
ApoI RAATTY 2 cut(s) 113, 517
Asp700I GAANNNNTTC 2 cut(s) 471, 951
AspLEI GCGC 2 cut(s) 289, 361
AsuHPI GGTGA 1 cut(s) 1033
AsuNHI GCTAGC 1 cut(s) 637
AvaI CYCGRG 1 cut(s) 1067
BaeGI GKGCMC 2 cut(s) 348, 1075
BalI TGGCCA 1 cut(s) 555
BanI GGYRCC 1 cut(s) 1072
BbsI GAAGAC 1 cut(s) 581
Bbv12I GWGCWC 1 cut(s) 348
BbvCI CCTCAGC 1 cut(s) 1331
BbvI GCAGC 4 cut(s) 442, 1028, 1125, 1197
BccI CCATC 3 cut(s) 417, 879, 1184
BceAI ACGGC 1 cut(s) 1151
BciT130I CCWGG 1 cut(s) 1254
BcoDI GTCTC 2 cut(s) 365, 1054
BfaI CTAG 9 cut(s) 546, 638, 698, 882, 905, 1020, 1214, 1412, 1425
BfmI CTRYAG 1 cut(s) 927
BfrI CTTAAG 1 cut(s) 1322
BisI GCNGC 6 cut(s) 431, 965, 1017, 1139, 1211, 1421
BlsI GCNGC 6 cut(s) 432, 966, 1018, 1140, 1212, 1422
Bme1390I CCNGG 1 cut(s) 1254
BmeT110I CYCGRG 1 cut(s) 1067
BmiI GGNNCC 1 cut(s) 1074
BmrFI CCNGG 1 cut(s) 1254
BmrI ACTGGG 1 cut(s) 1014
BmsI GCATC 2 cut(s) 653, 1317
BmtI GCTAGC 1 cut(s) 641
BmuI ACTGGG 1 cut(s) 1014
BpiI GAAGAC 1 cut(s) 581
Bpu10I CCTNAGC 1 cut(s) 1331
BsaBI GATNNNNATC 1 cut(s) 977
BsaHI GRCGYC 1 cut(s) 374
BsaJI CCNNGG 3 cut(s) 677, 828, 1253
BsaWI WCCGGW 2 cut(s) 771, 1434
Bse1I ACTGG 2 cut(s) 990, 1009
Bse3DI GCAATG 2 cut(s) 397, 628
Bse8I GATNNNNATC 1 cut(s) 977
BseBI CCWGG 1 cut(s) 1254
BseDI CCNNGG 3 cut(s) 677, 828, 1253
BseGI GGATG 6 cut(s) 107, 405, 409, 668, 1029, 1195
BseJI GATNNNNATC 1 cut(s) 977
BseMI GCAATG 2 cut(s) 397, 628
BseMII CTCAG 3 cut(s) 392, 656, 1322
BseNI ACTGG 2 cut(s) 990, 1009
BseRI GAGGAG 3 cut(s) 57, 694, 697
BseSI GKGCMC 2 cut(s) 348, 1075
BseXI GCAGC 4 cut(s) 442, 1028, 1125, 1197
Bsh1236I CGCG 1 cut(s) 967
BshFI GGCC 2 cut(s) 555, 1252
BshNI GGYRCC 1 cut(s) 1072
BsiHKAI GWGCWC 1 cut(s) 348
BsiHKCI CYCGRG 1 cut(s) 1067
BsiSI CCGG 2 cut(s) 772, 1435
BslFI GGGAC 5 cut(s) 550, 1015, 1021, 1094, 1303
BsmAI GTCTC 2 cut(s) 365, 1054
BsmBI CGTCTC 1 cut(s) 365
BsmFI GGGAC 5 cut(s) 550, 1015, 1021, 1094, 1303
BsmI GAATGC 1 cut(s) 1309
BsnI GGCC 2 cut(s) 555, 1252
BsoBI CYCGRG 1 cut(s) 1067
Bsp1286I GDGCHC 2 cut(s) 348, 1075
Bsp143I GATC 4 cut(s) 333, 489, 667, 972
BspACI CCGC 4 cut(s) 449, 822, 965, 1421
BspANI GGCC 2 cut(s) 555, 1252
BspCNI CTCAG 3 cut(s) 391, 655, 1323
BspFNI CGCG 1 cut(s) 967
BspLI GGNNCC 1 cut(s) 1074
BspMAI CTGCAG 1 cut(s) 931
BspOI GCTAGC 1 cut(s) 641
BspPI GGATC 1 cut(s) 662
BspQI GCTCTTC 2 cut(s) 87, 1101
BspT107I GGYRCC 1 cut(s) 1072
BspTI CTTAAG 1 cut(s) 1322
BsrBI CCGCTC 1 cut(s) 824
BsrDI GCAATG 2 cut(s) 397, 628
BsrI ACTGG 2 cut(s) 990, 1009
BssECI CCNNGG 3 cut(s) 677, 828, 1253
BssMI GATC 4 cut(s) 333, 489, 667, 972
BssNI GRCGYC 1 cut(s) 374
BssT1I CCWWGG 1 cut(s) 828
Bst2UI CCWGG 1 cut(s) 1254
Bst4CI ACNGT 3 cut(s) 194, 220, 305
Bst6I CTCTTC 3 cut(s) 87, 462, 1101
BstACI GRCGYC 1 cut(s) 374
BstAFI CTTAAG 1 cut(s) 1322
BstC8I GCNNGC 4 cut(s) 447, 562, 639, 719
BstDEI CTNAG 3 cut(s) 378, 642, 1331
BstF5I GGATG 6 cut(s) 107, 405, 409, 668, 1029, 1195
BstFNI CGCG 1 cut(s) 967
BstHHI GCGC 2 cut(s) 289, 361
BstKTI GATC 4 cut(s) 336, 492, 670, 975
BstMAI GTCTC 2 cut(s) 365, 1054
BstMBI GATC 4 cut(s) 333, 489, 667, 972
BstMWI GCNNNNNNNGC 3 cut(s) 561, 566, 618
BstNI CCWGG 1 cut(s) 1254
BstSCI CCNGG 1 cut(s) 1252
BstSFI CTRYAG 1 cut(s) 927
BstSLI GKGCMC 2 cut(s) 348, 1075
BstUI CGCG 1 cut(s) 967
BstV1I GCAGC 4 cut(s) 442, 1028, 1125, 1197
BstV2I GAAGAC 1 cut(s) 581
BsuRI GGCC 2 cut(s) 555, 1252
BtsCI GGATG 6 cut(s) 107, 405, 409, 668, 1029, 1195
Cac8I GCNNGC 4 cut(s) 447, 562, 639, 719
CfoI GCGC 2 cut(s) 289, 361
CseI GACGC 1 cut(s) 382
Csp6I GTAC 2 cut(s) 306, 1336
CviQI GTAC 2 cut(s) 306, 1336
DdeI CTNAG 3 cut(s) 378, 642, 1331
DpnI GATC 4 cut(s) 335, 491, 669, 974
DpnII GATC 4 cut(s) 333, 489, 667, 972
EaeI YGGCCR 1 cut(s) 553
Eam1104I CTCTTC 3 cut(s) 87, 462, 1101
EarI CTCTTC 3 cut(s) 87, 462, 1101
Eco130I CCWWGG 1 cut(s) 828
Eco147I AGGCCT 1 cut(s) 1252
Eco32I GATATC 1 cut(s) 616
Eco88I CYCGRG 1 cut(s) 1067
EcoRII CCWGG 1 cut(s) 1252
EcoRV GATATC 1 cut(s) 616
EcoT14I CCWWGG 1 cut(s) 828
ErhI CCWWGG 1 cut(s) 828
Esp3I CGTCTC 1 cut(s) 365
FalI AAGNNNNNCTT 2 cut(s) 821, 853
FaqI GGGAC 5 cut(s) 550, 1015, 1021, 1094, 1303
Fnu4HI GCNGC 6 cut(s) 431, 965, 1017, 1139, 1211, 1421
FokI GGATG 6 cut(s) 94, 392, 396, 675, 1036, 1202
Fsp4HI GCNGC 6 cut(s) 431, 965, 1017, 1139, 1211, 1421
FspBI CTAG 9 cut(s) 546, 638, 698, 882, 905, 1020, 1214, 1412, 1425
FspI TGCGCA 1 cut(s) 288
GlaI GCGC 2 cut(s) 288, 360
GluI GCNGC 6 cut(s) 431, 965, 1017, 1139, 1211, 1421
HaeIII GGCC 2 cut(s) 555, 1252
HapII CCGG 2 cut(s) 772, 1435
HgaI GACGC 1 cut(s) 382
HhaI GCGC 2 cut(s) 289, 361
Hin1I GRCGYC 1 cut(s) 374
Hin6I GCGC 2 cut(s) 287, 359
HinP1I GCGC 2 cut(s) 287, 359
HpaII CCGG 2 cut(s) 772, 1435
HphI GGTGA 1 cut(s) 1033
Hpy166II GTNNAC 2 cut(s) 346, 1103
Hpy188III TCNNGA 6 cut(s) 212, 487, 905, 1160, 1187, 1412
Hpy8I GTNNAC 2 cut(s) 346, 1103
Hpy99I CGWCG 1 cut(s) 280
HpyAV CCTTC 1 cut(s) 160
HpyCH4III ACNGT 3 cut(s) 194, 220, 305
HpyCH4IV ACGT 3 cut(s) 251, 275, 308
HpyCH4V TGCA 8 cut(s) 156, 346, 621, 792, 812, 892, 929, 1016
HpyF10VI GCNNNNNNNGC 3 cut(s) 561, 566, 618
HpyF3I CTNAG 3 cut(s) 378, 642, 1331
HpySE526I ACGT 3 cut(s) 251, 275, 308
Hsp92I GRCGYC 1 cut(s) 374
HspAI GCGC 2 cut(s) 287, 359
Kzo9I GATC 4 cut(s) 333, 489, 667, 972
LguI GCTCTTC 2 cut(s) 87, 1101
LmnI GCTCC 3 cut(s) 527, 756, 829
Lsp1109I GCAGC 4 cut(s) 442, 1028, 1125, 1197
LweI GCATC 2 cut(s) 653, 1317
MaeI CTAG 9 cut(s) 546, 638, 698, 882, 905, 1020, 1214, 1412, 1425
MaeII ACGT 3 cut(s) 251, 275, 308
MaeIII GTNAC 5 cut(s) 97, 139, 205, 864, 1039
MalI GATC 4 cut(s) 335, 491, 669, 974
MbiI CCGCTC 1 cut(s) 824
MboI GATC 4 cut(s) 333, 489, 667, 972
MboII GAAGA 9 cut(s) 74, 479, 586, 599, 829, 1088, 1138, 1273, 1307
MfeI CAATTG 1 cut(s) 93
MhlI GDGCHC 2 cut(s) 348, 1075
MlsI TGGCCA 1 cut(s) 555
MluCI AATT 8 cut(s) 57, 93, 113, 157, 517, 817, 859, 914
MluNI TGGCCA 1 cut(s) 555
MlyI GAGTC 6 cut(s) 347, 746, 787, 902, 1098, 1325
MmeI TCCRAC 1 cut(s) 259
Mox20I TGGCCA 1 cut(s) 555
MroXI GAANNNNTTC 2 cut(s) 471, 951
MscI TGGCCA 1 cut(s) 555
MseI TTAA 5 cut(s) 246, 296, 386, 1080, 1323
MslI CAYNNNNRTG 1 cut(s) 438
Msp20I TGGCCA 1 cut(s) 555
MspCI CTTAAG 1 cut(s) 1322
MspI CCGG 2 cut(s) 772, 1435
MspR9I CCNGG 1 cut(s) 1254
MunI CAATTG 1 cut(s) 93
Mva1269I GAATGC 1 cut(s) 1309
MvaI CCWGG 1 cut(s) 1254
MvnI CGCG 1 cut(s) 967
MwoI GCNNNNNNNGC 3 cut(s) 561, 566, 618
NdeII GATC 4 cut(s) 333, 489, 667, 972
NheI GCTAGC 1 cut(s) 637
NlaIV GGNNCC 1 cut(s) 1074
NmeAIII GCCGAG 1 cut(s) 1031
NmuCI GTSAC 3 cut(s) 205, 864, 1039
NsbI TGCGCA 1 cut(s) 288
PceI AGGCCT 1 cut(s) 1252
PciSI GCTCTTC 2 cut(s) 87, 1101
PcsI WCGNNNNNNNCGW 1 cut(s) 609
PctI GAATGC 1 cut(s) 1309
PdmI GAANNNNTTC 2 cut(s) 471, 951
PfeI GAWTC 7 cut(s) 223, 260, 350, 472, 919, 978, 1168
PflFI GACNNNGTC 1 cut(s) 1220
PkrI GCNGC 6 cut(s) 432, 966, 1018, 1140, 1212, 1422
PleI GAGTC 6 cut(s) 346, 745, 786, 902, 1098, 1324
PpsI GAGTC 6 cut(s) 346, 745, 786, 902, 1098, 1324
Psp6I CCWGG 1 cut(s) 1252
PspGI CCWGG 1 cut(s) 1252
PspN4I GGNNCC 1 cut(s) 1074
PstI CTGCAG 1 cut(s) 931
PsyI GACNNNGTC 1 cut(s) 1220
RsaI GTAC 2 cut(s) 307, 1337
RsaNI GTAC 2 cut(s) 306, 1336
RseI CAYNNNNRTG 1 cut(s) 438
SapI GCTCTTC 2 cut(s) 87, 1101
SaqAI TTAA 5 cut(s) 246, 296, 386, 1080, 1323
SatI GCNGC 6 cut(s) 431, 965, 1017, 1139, 1211, 1421
Sau3AI GATC 4 cut(s) 333, 489, 667, 972
SchI GAGTC 6 cut(s) 347, 746, 787, 902, 1098, 1325
ScrFI CCNGG 1 cut(s) 1254
SduI GDGCHC 2 cut(s) 348, 1075
SfaNI GCATC 2 cut(s) 653, 1317
SfcI CTRYAG 1 cut(s) 927
SmiMI CAYNNNNRTG 1 cut(s) 438
SmlI CTYRAG 1 cut(s) 1322
SmoI CTYRAG 1 cut(s) 1322
Sse9I AATT 8 cut(s) 57, 93, 113, 157, 517, 817, 859, 914
SseBI AGGCCT 1 cut(s) 1252
SsiI CCGC 4 cut(s) 449, 822, 965, 1421
SspMI CTAG 9 cut(s) 546, 638, 698, 882, 905, 1020, 1214, 1412, 1425
StuI AGGCCT 1 cut(s) 1252
StyD4I CCNGG 1 cut(s) 1252
StyI CCWWGG 1 cut(s) 828
TaaI ACNGT 3 cut(s) 194, 220, 305
TaiI ACGT 3 cut(s) 254, 278, 311
TaqI TCGA 3 cut(s) 213, 336, 1149
TasI AATT 8 cut(s) 57, 93, 113, 157, 517, 817, 859, 914
TauI GCSGC 2 cut(s) 967, 1423
TfiI GAWTC 7 cut(s) 223, 260, 350, 472, 919, 978, 1168
Tru1I TTAA 5 cut(s) 246, 296, 386, 1080, 1323
Tru9I TTAA 5 cut(s) 246, 296, 386, 1080, 1323
TseFI GTSAC 3 cut(s) 205, 864, 1039
TseI GCWGC 4 cut(s) 430, 1016, 1138, 1210
Tsp45I GTSAC 3 cut(s) 205, 864, 1039
TspDTI ATGAA 7 cut(s) 38, 125, 511, 587, 611, 647, 1098
Tth111I GACNNNGTC 1 cut(s) 1220
Vha464I CTTAAG 1 cut(s) 1322
VneI GTGCAC 1 cut(s) 344
XapI RAATTY 2 cut(s) 113, 517
XbaI TCTAGA 2 cut(s) 904, 1411
XcmI CCANNNNNNNNNTGG 1 cut(s) 1183
XmnI GAANNNNTTC 2 cut(s) 471, 951
XspI CTAG 9 cut(s) 546, 638, 698, 882, 905, 1020, 1214, 1412, 1425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.