Rh1BG240200

Putative S-adenosyl-L-methionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0016654
Physical Location & Seq
Forward (+)
36318824 .. 36320260
1437 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG240200.1

Sequence Viewer

Length: 1437 bp
ATGAGGAAACCAAGTGTTGTTATGAAGTTGGGAACATGGAGAGGAGTTCAGAGGTCAATTTTTATATTGGGTAGTTTTGCTCTTCTCATCTCAATTGTTACCTTATCATCCAAATTCTACTCTCTCAAATCATTTCATGTTACTGATACCTTCTGCAATTATGTAAATACTGATAGTCAAAGCAGGATTGACAGTCATAAAGTCACTCTCGAAACTGTGATTCAGAAACTCCAACAGGAACTTAAAACGTTCAGAGATTCAACAACAGATTCGTCGTCATCTGCGCATTTGTTAAAGCACAGTACGTTTCTTGCTGATATTTTGGGGCTGATCGAGTCGGTGCACGAATCTCTGGTGCAGAATGACGAGACGACTCAGAAGTTAAGCAATGATGTCAAAGACATCCATCCTCTGCTAAAACCGAAGCAGCACTCTGATGAGCCTGCGGAGTATTTTCTAATAGAAGAGATTCGGAAGTATGTCAAGATCAAGCCGAACAGATTGGGAAAGCAGAATTTCATGGGAGCTAATGGGACTTTCACTAGCATTGGCCATGCTTGCTTTGCCATGAAGACAGAACTTGAAGAATACATGAACTACGACATTGGAGATATCTGCAATGACGATTGGAAGCTAGCTCAGAAGCTCATGGTTCATGGATGCGATCCACTTCCAAGGAGGAGGTGCTTCGCTCTGGCCCCGAAGATATATAGCAAGCCACTCCCAATCCATGAGTCCATGTGGAAGCTCCCAGACAATAGAAATGTCCGGTGGAGTCAGTATCGGTGCAAGAACTTTACTTGCCTTGCAAGTAATTCCGCTCCCAAGGGGTTCTTCAAATGTGCAGATTGCTTCAATCTGTCACACCACGAAATGCCTAGATGGATGCAACAAGTAAGTCTAGACTCAAAATTGAATCTGACTGCAGATTTTCTTATCCCCGAAGTGCTTCAAATCAAGCCGCGAGAGATCAGAATCGGACTGGATTTTAGCGTAGGGACTGGGACTTTTGCAGCTAGGATGAGGGAGTTCAATGTCACCATTGTCTCGGCAACTATCAATCTTGGGGCACCTTTTAATGAAATGATAGCTCTTCGTGGACTCGTCCCTCTTTACTTGACAATCAATCAACGGCTGCCATTCTTCGACAACACTCTGGATTTGATTCACACCACAAGATTTCTGGATGGCTGGATTGATTTTGTGCTGCTAGACTTTGTCTTGTATGATTGGGATAGAGTTTTGAGGCCTGGGGGTTTGCTGTGGATTGATAGCTTCTTCTGTGTGAAGGAAGATTTGAATGCTTATTTGGAGTCCCTTAAGATGCTGAGGTACAAGAAGCACAAATGGGTTGTTGTTCCAAAGATTGATAAAGATGATGATAGAGAGGTTTTCTTTTCTGCTGTTCTAGAAAAGCCGCCCAGACCATTCCGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29 zf-RING_2

Protein Analysis

478

Amino Acids

55.27

Weight (kDa)

8.74

Isoelectric Point (pI)

37.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 318 - 441 5.8e-06 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016654)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G01240
fragaria_vesca FvH4_7g15510
malus_domestica MD07G1130500.v1.1
prunus_persica Prupe.2G178700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0357841
rosa_laevigata RLG00000027992
rosa_multiflora Rmu_sc0002071.1_g000002 Rmu_sc0011109.1_g000006
rosa_roxburghii Rroxscaffold_4G00298460
rosa_rugosa Rorug01G0258900
rosa_samantha Rh1AG272900 Rh1BG240200 Rh1DG268500
rosa_wichuraiana Rw1G024360

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 285
AccB1I GGYRCC 1 cut(s) 1069
AccBSI CCGCTC 1 cut(s) 821
AccII CGCG 1 cut(s) 964
AciI CCGC 4 cut(s) 446, 819, 962, 1418
AclI AACGTT 1 cut(s) 248
AclWI GGATC 1 cut(s) 659
AcoI YGGCCR 1 cut(s) 550
AcsI RAATTY 2 cut(s) 113, 514
AfaI GTAC 2 cut(s) 304, 1334
AflII CTTAAG 1 cut(s) 1319
AgsI TTSAA 8 cut(s) 261, 584, 838, 856, 916, 953, 1033, 1300
AjnI CCWGG 1 cut(s) 1249
AjuI GAANNNNNNNTTGG 4 cut(s) 818, 850, 1292, 1324
AluBI AGCT 8 cut(s) 527, 634, 638, 646, 748, 1016, 1091, 1275
AluI AGCT 8 cut(s) 527, 634, 638, 646, 748, 1016, 1091, 1275
Alw21I GWGCWC 1 cut(s) 345
Alw26I GTCTC 2 cut(s) 362, 1051
Alw44I GTGCAC 1 cut(s) 341
AlwI GGATC 1 cut(s) 659
AoxI GGCC 3 cut(s) 550, 696, 1247
ApaLI GTGCAC 1 cut(s) 341
ApeKI GCWGC 4 cut(s) 427, 1013, 1135, 1207
ApoI RAATTY 2 cut(s) 113, 514
Asp700I GAANNNNTTC 2 cut(s) 468, 948
AspLEI GCGC 1 cut(s) 286
AspS9I GGNCC 1 cut(s) 697
AsuHPI GGTGA 1 cut(s) 1030
AsuNHI GCTAGC 1 cut(s) 634
BaeGI GKGCMC 2 cut(s) 345, 1072
BalI TGGCCA 1 cut(s) 552
BanI GGYRCC 1 cut(s) 1069
BbsI GAAGAC 1 cut(s) 578
Bbv12I GWGCWC 1 cut(s) 345
BbvCI CCTCAGC 1 cut(s) 1328
BbvI GCAGC 4 cut(s) 439, 1025, 1122, 1194
BccI CCATC 3 cut(s) 414, 876, 1181
BceAI ACGGC 1 cut(s) 1148
BciT130I CCWGG 1 cut(s) 1251
BcoDI GTCTC 2 cut(s) 362, 1051
BfaI CTAG 7 cut(s) 543, 635, 879, 902, 1017, 1211, 1409
BfmI CTRYAG 1 cut(s) 924
BfrI CTTAAG 1 cut(s) 1319
BisI GCNGC 6 cut(s) 428, 962, 1014, 1136, 1208, 1418
BlsI GCNGC 6 cut(s) 429, 963, 1015, 1137, 1209, 1419
Bme1390I CCNGG 1 cut(s) 1251
BmgT120I GGNCC 1 cut(s) 697
BmiI GGNNCC 2 cut(s) 699, 1071
BmrFI CCNGG 1 cut(s) 1251
BmrI ACTGGG 1 cut(s) 1011
BmsI GCATC 3 cut(s) 650, 876, 1314
BmtI GCTAGC 1 cut(s) 638
BmuI ACTGGG 1 cut(s) 1011
BpiI GAAGAC 1 cut(s) 578
Bpu10I CCTNAGC 1 cut(s) 1328
BsaBI GATNNNNATC 1 cut(s) 974
BsaJI CCNNGG 3 cut(s) 674, 825, 1250
BsaWI WCCGGW 2 cut(s) 768, 1431
Bse1I ACTGG 2 cut(s) 987, 1006
Bse3DI GCAATG 2 cut(s) 394, 625
Bse8I GATNNNNATC 1 cut(s) 974
BseBI CCWGG 1 cut(s) 1251
BseDI CCNNGG 3 cut(s) 674, 825, 1250
BseGI GGATG 7 cut(s) 107, 402, 406, 665, 891, 1026, 1192
BseJI GATNNNNATC 1 cut(s) 974
BseMI GCAATG 2 cut(s) 394, 625
BseMII CTCAG 3 cut(s) 389, 653, 1319
BseNI ACTGG 2 cut(s) 987, 1006
BseRI GAGGAG 2 cut(s) 57, 694
BseSI GKGCMC 2 cut(s) 345, 1072
BseXI GCAGC 4 cut(s) 439, 1025, 1122, 1194
BsgI GTGCAG 2 cut(s) 377, 864
Bsh1236I CGCG 1 cut(s) 964
BshFI GGCC 3 cut(s) 552, 698, 1249
BshNI GGYRCC 1 cut(s) 1069
BsiHKAI GWGCWC 1 cut(s) 345
BsiSI CCGG 2 cut(s) 769, 1432
BslFI GGGAC 5 cut(s) 547, 1012, 1018, 1091, 1300
BsmAI GTCTC 2 cut(s) 362, 1051
BsmBI CGTCTC 1 cut(s) 362
BsmFI GGGAC 5 cut(s) 547, 1012, 1018, 1091, 1300
BsmI GAATGC 1 cut(s) 1306
BsnI GGCC 3 cut(s) 552, 698, 1249
Bsp1286I GDGCHC 2 cut(s) 345, 1072
Bsp143I GATC 4 cut(s) 330, 486, 664, 969
BspACI CCGC 4 cut(s) 446, 819, 962, 1418
BspANI GGCC 3 cut(s) 552, 698, 1249
BspCNI CTCAG 3 cut(s) 388, 652, 1320
BspFNI CGCG 1 cut(s) 964
BspLI GGNNCC 2 cut(s) 699, 1071
BspMAI CTGCAG 1 cut(s) 928
BspOI GCTAGC 1 cut(s) 638
BspPI GGATC 1 cut(s) 659
BspQI GCTCTTC 2 cut(s) 87, 1098
BspT107I GGYRCC 1 cut(s) 1069
BspTI CTTAAG 1 cut(s) 1319
BsrBI CCGCTC 1 cut(s) 821
BsrDI GCAATG 2 cut(s) 394, 625
BsrI ACTGG 2 cut(s) 987, 1006
BssECI CCNNGG 3 cut(s) 674, 825, 1250
BssMI GATC 4 cut(s) 330, 486, 664, 969
BssT1I CCWWGG 2 cut(s) 674, 825
Bst2UI CCWGG 1 cut(s) 1251
Bst4CI ACNGT 3 cut(s) 194, 217, 302
Bst6I CTCTTC 3 cut(s) 87, 459, 1098
BstAFI CTTAAG 1 cut(s) 1319
BstC8I GCNNGC 4 cut(s) 444, 559, 636, 716
BstDEI CTNAG 3 cut(s) 375, 639, 1328
BstF5I GGATG 7 cut(s) 107, 402, 406, 665, 891, 1026, 1192
BstFNI CGCG 1 cut(s) 964
BstHHI GCGC 1 cut(s) 286
BstKTI GATC 4 cut(s) 333, 489, 667, 972
BstMAI GTCTC 2 cut(s) 362, 1051
BstMBI GATC 4 cut(s) 330, 486, 664, 969
BstMWI GCNNNNNNNGC 2 cut(s) 558, 563
BstNI CCWGG 1 cut(s) 1251
BstSCI CCNGG 1 cut(s) 1249
BstSFI CTRYAG 1 cut(s) 924
BstSLI GKGCMC 2 cut(s) 345, 1072
BstUI CGCG 1 cut(s) 964
BstV1I GCAGC 4 cut(s) 439, 1025, 1122, 1194
BstV2I GAAGAC 1 cut(s) 578
BsuRI GGCC 3 cut(s) 552, 698, 1249
BtsCI GGATG 7 cut(s) 107, 402, 406, 665, 891, 1026, 1192
Cac8I GCNNGC 4 cut(s) 444, 559, 636, 716
CfoI GCGC 1 cut(s) 286
Cfr13I GGNCC 1 cut(s) 697
Csp6I GTAC 2 cut(s) 303, 1333
CviQI GTAC 2 cut(s) 303, 1333
DdeI CTNAG 3 cut(s) 375, 639, 1328
DpnI GATC 4 cut(s) 332, 488, 666, 971
DpnII GATC 4 cut(s) 330, 486, 664, 969
EaeI YGGCCR 1 cut(s) 550
Eam1104I CTCTTC 3 cut(s) 87, 459, 1098
EarI CTCTTC 3 cut(s) 87, 459, 1098
Eco130I CCWWGG 2 cut(s) 674, 825
Eco147I AGGCCT 1 cut(s) 1249
Eco32I GATATC 1 cut(s) 613
EcoRII CCWGG 1 cut(s) 1249
EcoRV GATATC 1 cut(s) 613
EcoT14I CCWWGG 2 cut(s) 674, 825
ErhI CCWWGG 2 cut(s) 674, 825
Esp3I CGTCTC 1 cut(s) 362
FalI AAGNNNNNCTT 2 cut(s) 818, 850
FaqI GGGAC 5 cut(s) 547, 1012, 1018, 1091, 1300
Fnu4HI GCNGC 6 cut(s) 428, 962, 1014, 1136, 1208, 1418
FokI GGATG 7 cut(s) 94, 389, 393, 672, 898, 1033, 1199
Fsp4HI GCNGC 6 cut(s) 428, 962, 1014, 1136, 1208, 1418
FspBI CTAG 7 cut(s) 543, 635, 879, 902, 1017, 1211, 1409
FspI TGCGCA 1 cut(s) 285
GlaI GCGC 1 cut(s) 285
GluI GCNGC 6 cut(s) 428, 962, 1014, 1136, 1208, 1418
HaeIII GGCC 3 cut(s) 552, 698, 1249
HapII CCGG 2 cut(s) 769, 1432
HhaI GCGC 1 cut(s) 286
Hin6I GCGC 1 cut(s) 284
HinP1I GCGC 1 cut(s) 284
HpaII CCGG 2 cut(s) 769, 1432
HphI GGTGA 1 cut(s) 1030
Hpy166II GTNNAC 2 cut(s) 343, 1100
Hpy188III TCNNGA 6 cut(s) 209, 484, 902, 1157, 1184, 1409
Hpy8I GTNNAC 2 cut(s) 343, 1100
Hpy99I CGWCG 1 cut(s) 277
HpyAV CCTTC 2 cut(s) 160, 1282
HpyCH4III ACNGT 3 cut(s) 194, 217, 302
HpyCH4IV ACGT 2 cut(s) 248, 305
HpyF10VI GCNNNNNNNGC 2 cut(s) 558, 563
HpyF3I CTNAG 3 cut(s) 375, 639, 1328
HpySE526I ACGT 2 cut(s) 248, 305
HspAI GCGC 1 cut(s) 284
Kzo9I GATC 4 cut(s) 330, 486, 664, 969
LguI GCTCTTC 2 cut(s) 87, 1098
LmnI GCTCC 3 cut(s) 524, 753, 826
Lsp1109I GCAGC 4 cut(s) 439, 1025, 1122, 1194
LweI GCATC 3 cut(s) 650, 876, 1314
MaeI CTAG 7 cut(s) 543, 635, 879, 902, 1017, 1211, 1409
MaeII ACGT 2 cut(s) 248, 305
MaeIII GTNAC 5 cut(s) 97, 139, 202, 861, 1036
MalI GATC 4 cut(s) 332, 488, 666, 971
MbiI CCGCTC 1 cut(s) 821
MboI GATC 4 cut(s) 330, 486, 664, 969
MfeI CAATTG 1 cut(s) 93
MhlI GDGCHC 2 cut(s) 345, 1072
MlsI TGGCCA 1 cut(s) 552
MluCI AATT 7 cut(s) 57, 93, 113, 157, 514, 814, 911
MluNI TGGCCA 1 cut(s) 552
MlyI GAGTC 7 cut(s) 344, 367, 743, 784, 899, 1095, 1322
MmeI TCCRAC 1 cut(s) 256
Mox20I TGGCCA 1 cut(s) 552
MroXI GAANNNNTTC 2 cut(s) 468, 948
MscI TGGCCA 1 cut(s) 552
MseI TTAA 5 cut(s) 243, 293, 383, 1077, 1320
MslI CAYNNNNRTG 1 cut(s) 435
Msp20I TGGCCA 1 cut(s) 552
MspCI CTTAAG 1 cut(s) 1319
MspI CCGG 2 cut(s) 769, 1432
MspR9I CCNGG 1 cut(s) 1251
MunI CAATTG 1 cut(s) 93
Mva1269I GAATGC 1 cut(s) 1306
MvaI CCWGG 1 cut(s) 1251
MvnI CGCG 1 cut(s) 964
MwoI GCNNNNNNNGC 2 cut(s) 558, 563
NdeII GATC 4 cut(s) 330, 486, 664, 969
NheI GCTAGC 1 cut(s) 634
NlaIV GGNNCC 2 cut(s) 699, 1071
NmeAIII GCCGAG 1 cut(s) 1028
NmuCI GTSAC 3 cut(s) 202, 861, 1036
NsbI TGCGCA 1 cut(s) 285
PceI AGGCCT 1 cut(s) 1249
PciSI GCTCTTC 2 cut(s) 87, 1098
PctI GAATGC 1 cut(s) 1306
PdmI GAANNNNTTC 2 cut(s) 468, 948
PfeI GAWTC 8 cut(s) 220, 257, 269, 347, 469, 916, 975, 1165
PflFI GACNNNGTC 1 cut(s) 1217
PkrI GCNGC 6 cut(s) 429, 963, 1015, 1137, 1209, 1419
PleI GAGTC 7 cut(s) 343, 367, 742, 783, 899, 1095, 1321
PpsI GAGTC 7 cut(s) 343, 367, 742, 783, 899, 1095, 1321
Psp1406I AACGTT 1 cut(s) 248
Psp6I CCWGG 1 cut(s) 1249
PspGI CCWGG 1 cut(s) 1249
PspN4I GGNNCC 2 cut(s) 699, 1071
PspPI GGNCC 1 cut(s) 697
PstI CTGCAG 1 cut(s) 928
PsyI GACNNNGTC 1 cut(s) 1217
RsaI GTAC 2 cut(s) 304, 1334
RsaNI GTAC 2 cut(s) 303, 1333
RseI CAYNNNNRTG 1 cut(s) 435
SapI GCTCTTC 2 cut(s) 87, 1098
SaqAI TTAA 5 cut(s) 243, 293, 383, 1077, 1320
SatI GCNGC 6 cut(s) 428, 962, 1014, 1136, 1208, 1418
Sau3AI GATC 4 cut(s) 330, 486, 664, 969
Sau96I GGNCC 1 cut(s) 697
SchI GAGTC 7 cut(s) 344, 367, 743, 784, 899, 1095, 1322
ScrFI CCNGG 1 cut(s) 1251
SduI GDGCHC 2 cut(s) 345, 1072
SfaNI GCATC 3 cut(s) 650, 876, 1314
SfcI CTRYAG 1 cut(s) 924
SmiMI CAYNNNNRTG 1 cut(s) 435
SmlI CTYRAG 1 cut(s) 1319
SmoI CTYRAG 1 cut(s) 1319
Sse9I AATT 7 cut(s) 57, 93, 113, 157, 514, 814, 911
SseBI AGGCCT 1 cut(s) 1249
SsiI CCGC 4 cut(s) 446, 819, 962, 1418
SspMI CTAG 7 cut(s) 543, 635, 879, 902, 1017, 1211, 1409
StuI AGGCCT 1 cut(s) 1249
StyD4I CCNGG 1 cut(s) 1249
StyI CCWWGG 2 cut(s) 674, 825
TaaI ACNGT 3 cut(s) 194, 217, 302
TaiI ACGT 2 cut(s) 251, 308
TaqI TCGA 3 cut(s) 210, 333, 1146
TasI AATT 7 cut(s) 57, 93, 113, 157, 514, 814, 911
TauI GCSGC 2 cut(s) 964, 1420
TfiI GAWTC 8 cut(s) 220, 257, 269, 347, 469, 916, 975, 1165
Tru1I TTAA 5 cut(s) 243, 293, 383, 1077, 1320
Tru9I TTAA 5 cut(s) 243, 293, 383, 1077, 1320
TseFI GTSAC 3 cut(s) 202, 861, 1036
TseI GCWGC 4 cut(s) 427, 1013, 1135, 1207
Tsp45I GTSAC 3 cut(s) 202, 861, 1036
TspDTI ATGAA 7 cut(s) 38, 125, 508, 584, 608, 644, 1095
Tth111I GACNNNGTC 1 cut(s) 1217
Vha464I CTTAAG 1 cut(s) 1319
VneI GTGCAC 1 cut(s) 341
XapI RAATTY 2 cut(s) 113, 514
XbaI TCTAGA 2 cut(s) 901, 1408
XcmI CCANNNNNNNNNTGG 1 cut(s) 1180
XmnI GAANNNNTTC 2 cut(s) 468, 948
XspI CTAG 7 cut(s) 543, 635, 879, 902, 1017, 1211, 1409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.