FvH4_7g20920

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0008283
Physical Location & Seq
Forward (+)
16910980 .. 16916336
5357 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g20920.t2

Sequence Viewer

Length: 1053 bp
ATGAAGCTACAAATATCGGGGCAACCGGTGAAACGTCCCATTTCTGTTCTTCTTTTTCCGGTGAATTTTGTAACCCGCCAAATTTTCCAGCGCGACGGCGTTACGGAGAGGAAGACGGAGATGACTGCGCTACCGTACGGCGACGTGGATTCCCATCTGAAAGCAATGGCCGGTAAGGCCGAGGGCTTCGGCCGGTTCGCCATCGGAGGCCTCCACGGCCCCGTCTATTCCGTCACCACATTGGCAGATGATGGTCCAGGCTCACTTCGTGAAGGATGCCGGAAACAAGAACCACTCTGGATTCTCTTTGAGGTTTCAGGCACCATTCATCTCTCTTCTTATTTGAGCGTTTCATCTTACAAGACAATTGATGGCCGGGGGCGAAGAGTAAAGCTCACAGGCAAGGGCCTTAGACTGAAGGAATGTGAACACATAATCATATGCAACCTTGAGTTTGAAGGTGGTAGAGGACATGATGTAGATGGCATTCAGATAAAACCAAATTCCAGGCATATATGGATTGACCGATGCAGCCTTCGTGATTATGATGATGGTCTCATAGATATCACCCGACAAAGTACAGACATAACTATTTCTAGATGCATTCGGGTCACAATTCATCATTGTTTTTTTGACGGGACAAGACAAAGGCAACCTCGTGTTAGATTTGGGAAAGTTCATCTTTACAACAATTACACTCGTAACTGGGGTATATATGCTGTTTGTGCCAGCGTAGAATCCCAGATATACTCTCAATGCAACATATATGAAGCAGGAACGAAGAAGAAGACTTTTGAATATTATACTGAGAAGGCTGCAGATAGAGAAGAGGCGAAATCTGGCTTCATACGATCTGAGGGGGATGTATTCCTGAATGGAGCTCAACCATGCACATTAACAGGGTTCAGTGAAGACTGCATGTTCCATCCAAGTGAATTTTATCCAGTTTGGACTATGGAAGCAGCCTCAGATTCTCTTAAAGGTGTTCTCCACATTCTTACGGGTTGGCAACCCATTCAAAGGCCGCCATTAGAGCAGCCGATAATGGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

351

Amino Acids

39.58

Weight (kDa)

8.45

Isoelectric Point (pI)

43.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 115 - 285 4.3e-28 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 320
AccII CGCG 1 cut(s) 93
AciI CCGC 2 cut(s) 76, 1025
AcoI YGGCCR 3 cut(s) 168, 190, 373
AcsI RAATTY 4 cut(s) 64, 81, 502, 935
AcuI CTGAAG 1 cut(s) 437
AdeI CACNNNGTG 1 cut(s) 269
AfaI GTAC 2 cut(s) 137, 580
AfiI CCNNNNNNNGG 1 cut(s) 1020
AgeI ACCGGT 1 cut(s) 25
AgsI TTSAA 3 cut(s) 458, 797, 1019
AjiI CACGTC 1 cut(s) 145
AjnI CCWGG 2 cut(s) 256, 506
AluBI AGCT 3 cut(s) 7, 394, 881
AluI AGCT 3 cut(s) 7, 394, 881
Alw21I GWGCWC 1 cut(s) 883
Alw26I GTCTC 1 cut(s) 560
AoxI GGCC 8 cut(s) 168, 177, 190, 208, 217, 373, 406, 1022
ApeKI GCWGC 4 cut(s) 531, 815, 962, 1036
ApoI RAATTY 4 cut(s) 64, 81, 502, 935
AsiGI ACCGGT 1 cut(s) 25
AspLEI GCGC 2 cut(s) 93, 130
AspS9I GGNCC 3 cut(s) 218, 254, 406
AsuC2I CCSGG 1 cut(s) 377
AsuHPI GGTGA 4 cut(s) 40, 73, 226, 559
AvaII GGWCC 1 cut(s) 254
BanI GGYRCC 1 cut(s) 320
BanII GRGCYC 1 cut(s) 883
BauI CACGAG 1 cut(s) 657
BbsI GAAGAC 3 cut(s) 119, 794, 918
Bbv12I GWGCWC 1 cut(s) 883
BbvI GCAGC 4 cut(s) 543, 802, 974, 1048
BccI CCATC 7 cut(s) 162, 209, 245, 365, 476, 545, 933
BceAI ACGGC 3 cut(s) 112, 154, 232
BciT130I CCWGG 2 cut(s) 258, 508
BcnI CCSGG 1 cut(s) 377
BcoDI GTCTC 1 cut(s) 560
BfaI CTAG 1 cut(s) 597
BfmI CTRYAG 1 cut(s) 816
BglI GCCNNNNNGGC 2 cut(s) 176, 216
BisI GCNGC 5 cut(s) 532, 816, 963, 1025, 1037
BlsI GCNGC 5 cut(s) 533, 817, 964, 1026, 1038
Bme1390I CCNGG 3 cut(s) 258, 377, 508
Bme18I GGWCC 1 cut(s) 254
BmgBI CACGTC 1 cut(s) 145
BmgT120I GGNCC 3 cut(s) 218, 254, 406
BmiI GGNNCC 2 cut(s) 220, 322
BmrFI CCNGG 3 cut(s) 258, 377, 508
BmrI ACTGGG 1 cut(s) 715
BmsI GCATC 3 cut(s) 266, 518, 590
BmuI ACTGGG 1 cut(s) 715
BpiI GAAGAC 3 cut(s) 119, 794, 918
BplI GAGNNNNNCTC 2 cut(s) 378, 410
BpuEI CTTGAG 1 cut(s) 470
BpuMI CCSGG 1 cut(s) 377
BsaBI GATNNNNATC 1 cut(s) 153
BsaI GGTCTC 1 cut(s) 560
BsaJI CCNNGG 3 cut(s) 180, 214, 376
BsaWI WCCGGW 2 cut(s) 25, 58
Bsc4I CCNNNNNNNGG 1 cut(s) 1020
Bse118I RCCGGY 3 cut(s) 25, 170, 192
Bse1I ACTGG 2 cut(s) 710, 944
Bse3DI GCAATG 1 cut(s) 171
Bse8I GATNNNNATC 1 cut(s) 153
BseBI CCWGG 2 cut(s) 258, 508
BseDI CCNNGG 3 cut(s) 180, 214, 376
BseGI GGATG 3 cut(s) 281, 868, 925
BseJI GATNNNNATC 1 cut(s) 153
BseLI CCNNNNNNNGG 1 cut(s) 1020
BseMI GCAATG 1 cut(s) 171
BseMII CTCAG 3 cut(s) 798, 846, 981
BseNI ACTGG 2 cut(s) 710, 944
BseX3I CGGCCG 1 cut(s) 190
BseXI GCAGC 4 cut(s) 543, 802, 974, 1048
Bsh1236I CGCG 1 cut(s) 93
Bsh1285I CGRYCG 1 cut(s) 193
BshFI GGCC 8 cut(s) 170, 179, 192, 210, 219, 375, 408, 1024
BshNI GGYRCC 1 cut(s) 320
BshTI ACCGGT 1 cut(s) 25
BsiEI CGRYCG 1 cut(s) 193
BsiHKAI GWGCWC 1 cut(s) 883
BsiSI CCGG 6 cut(s) 26, 59, 171, 193, 280, 376
BsiWI CGTACG 1 cut(s) 135
BslFI GGGAC 2 cut(s) 21, 652
BslI CCNNNNNNNGG 1 cut(s) 1020
BsmAI GTCTC 1 cut(s) 560
BsmFI GGGAC 2 cut(s) 21, 652
BsmI GAATGC 2 cut(s) 486, 603
BsnI GGCC 8 cut(s) 170, 179, 192, 210, 219, 375, 408, 1024
Bso31I GGTCTC 1 cut(s) 560
Bsp1286I GDGCHC 1 cut(s) 883
Bsp143I GATC 1 cut(s) 851
BspACI CCGC 2 cut(s) 76, 1025
BspANI GGCC 8 cut(s) 170, 179, 192, 210, 219, 375, 408, 1024
BspCNI CTCAG 3 cut(s) 799, 847, 980
BspFNI CGCG 1 cut(s) 93
BspLI GGNNCC 2 cut(s) 220, 322
BspMAI CTGCAG 1 cut(s) 820
BspT107I GGYRCC 1 cut(s) 320
BspTNI GGTCTC 1 cut(s) 560
BsrDI GCAATG 1 cut(s) 171
BsrFI RCCGGY 3 cut(s) 25, 170, 192
BsrI ACTGG 2 cut(s) 710, 944
BssAI RCCGGY 3 cut(s) 25, 170, 192
BssECI CCNNGG 3 cut(s) 180, 214, 376
BssMI GATC 1 cut(s) 851
BssSI CACGAG 1 cut(s) 657
Bst2BI CACGAG 1 cut(s) 657
Bst2UI CCWGG 2 cut(s) 258, 508
Bst4CI ACNGT 1 cut(s) 135
Bst6I CTCTTC 3 cut(s) 340, 379, 822
BstC8I GCNNGC 1 cut(s) 730
BstDEI CTNAG 4 cut(s) 410, 807, 855, 967
BstDSI CCRYGG 1 cut(s) 214
BstF5I GGATG 3 cut(s) 281, 868, 925
BstFNI CGCG 1 cut(s) 93
BstHHI GCGC 2 cut(s) 93, 130
BstKTI GATC 1 cut(s) 854
BstMAI GTCTC 1 cut(s) 560
BstMBI GATC 1 cut(s) 851
BstMCI CGRYCG 1 cut(s) 193
BstMWI GCNNNNNNNGC 4 cut(s) 176, 216, 725, 1033
BstNI CCWGG 2 cut(s) 258, 508
BstNSI RCATGY 1 cut(s) 922
BstSCI CCNGG 3 cut(s) 256, 375, 506
BstSFI CTRYAG 1 cut(s) 816
BstUI CGCG 1 cut(s) 93
BstV1I GCAGC 4 cut(s) 543, 802, 974, 1048
BstV2I GAAGAC 3 cut(s) 119, 794, 918
BstZI CGGCCG 1 cut(s) 190
BsuRI GGCC 8 cut(s) 170, 179, 192, 210, 219, 375, 408, 1024
BtgI CCRYGG 1 cut(s) 214
BtrI CACGTC 1 cut(s) 145
BtsCI GGATG 3 cut(s) 281, 868, 925
BtsIMutI CAGTG 1 cut(s) 913
Cac8I GCNNGC 1 cut(s) 730
CfoI GCGC 2 cut(s) 93, 130
Cfr10I RCCGGY 3 cut(s) 25, 170, 192
Cfr13I GGNCC 3 cut(s) 218, 254, 406
Csp6I GTAC 2 cut(s) 136, 579
CspAI ACCGGT 1 cut(s) 25
CviAII CATG 3 cut(s) 473, 888, 919
CviQI GTAC 2 cut(s) 136, 579
DdeI CTNAG 4 cut(s) 410, 807, 855, 967
DpnI GATC 1 cut(s) 853
DpnII GATC 1 cut(s) 851
DraIII CACNNNGTG 1 cut(s) 269
EaeI YGGCCR 3 cut(s) 168, 190, 373
EagI CGGCCG 1 cut(s) 190
Eam1104I CTCTTC 3 cut(s) 340, 379, 822
EarI CTCTTC 3 cut(s) 340, 379, 822
Ecl136II GAGCTC 1 cut(s) 881
EclXI CGGCCG 1 cut(s) 190
Eco147I AGGCCT 1 cut(s) 210
Eco24I GRGCYC 1 cut(s) 883
Eco31I GGTCTC 1 cut(s) 560
Eco32I GATATC 1 cut(s) 565
Eco47I GGWCC 1 cut(s) 254
Eco52I CGGCCG 1 cut(s) 190
Eco53kI GAGCTC 1 cut(s) 881
Eco57I CTGAAG 1 cut(s) 437
EcoICRI GAGCTC 1 cut(s) 881
EcoO109I RGGNCCY 1 cut(s) 406
EcoRII CCWGG 2 cut(s) 256, 506
EcoRV GATATC 1 cut(s) 565
EcoT22I ATGCAT 1 cut(s) 605
EcoT38I GRGCYC 1 cut(s) 883
FaeI CATG 3 cut(s) 476, 891, 922
FalI AAGNNNNNCTT 2 cut(s) 666, 698
FaqI GGGAC 2 cut(s) 21, 652
FatI CATG 3 cut(s) 472, 887, 918
FauI CCCGC 1 cut(s) 83
FauNDI CATATG 1 cut(s) 440
Fnu4HI GCNGC 5 cut(s) 532, 816, 963, 1025, 1037
FokI GGATG 3 cut(s) 288, 875, 912
FriOI GRGCYC 1 cut(s) 883
Fsp4HI GCNGC 5 cut(s) 532, 816, 963, 1025, 1037
FspBI CTAG 1 cut(s) 597
GlaI GCGC 2 cut(s) 92, 129
GluI GCNGC 5 cut(s) 532, 816, 963, 1025, 1037
HaeIII GGCC 8 cut(s) 170, 179, 192, 210, 219, 375, 408, 1024
HapII CCGG 6 cut(s) 26, 59, 171, 193, 280, 376
HhaI GCGC 2 cut(s) 93, 130
Hin1II CATG 3 cut(s) 476, 891, 922
Hin6I GCGC 2 cut(s) 91, 128
HinP1I GCGC 2 cut(s) 91, 128
HinfI GANTC 4 cut(s) 149, 301, 737, 971
HpaII CCGG 6 cut(s) 26, 59, 171, 193, 280, 376
HphI GGTGA 4 cut(s) 40, 73, 226, 559
Hpy166II GTNNAC 1 cut(s) 428
Hpy188I TCNGA 5 cut(s) 159, 206, 492, 856, 970
Hpy188III TCNNGA 5 cut(s) 269, 298, 539, 597, 871
Hpy8I GTNNAC 1 cut(s) 428
Hpy99I CGWCG 2 cut(s) 98, 146
HpyAV CCTTC 5 cut(s) 266, 412, 452, 545, 805
HpyCH4III ACNGT 1 cut(s) 135
HpyCH4IV ACGT 2 cut(s) 34, 144
HpyCH4V TGCA 7 cut(s) 444, 531, 603, 759, 818, 891, 918
HpyF10VI GCNNNNNNNGC 4 cut(s) 176, 216, 725, 1033
HpyF3I CTNAG 4 cut(s) 410, 807, 855, 967
HpySE526I ACGT 2 cut(s) 34, 144
Hsp92II CATG 3 cut(s) 476, 891, 922
HspAI GCGC 2 cut(s) 91, 128
Kzo9I GATC 1 cut(s) 851
LmnI GCTCC 1 cut(s) 878
Lsp1109I GCAGC 4 cut(s) 543, 802, 974, 1048
LweI GCATC 3 cut(s) 266, 518, 590
MaeI CTAG 1 cut(s) 597
MaeII ACGT 2 cut(s) 34, 144
MaeIII GTNAC 5 cut(s) 70, 100, 232, 610, 701
MalI GATC 1 cut(s) 853
MboI GATC 1 cut(s) 851
MboII GAAGA 9 cut(s) 41, 124, 327, 396, 793, 796, 799, 839, 923
MfeI CAATTG 1 cut(s) 366
MhlI GDGCHC 1 cut(s) 883
MluCI AATT 7 cut(s) 64, 81, 366, 502, 615, 691, 935
Mph1103I ATGCAT 1 cut(s) 605
MseI TTAA 2 cut(s) 896, 978
MslI CAYNNNNRTG 1 cut(s) 930
MspI CCGG 6 cut(s) 26, 59, 171, 193, 280, 376
MspR9I CCNGG 3 cut(s) 258, 377, 508
MunI CAATTG 1 cut(s) 366
Mva1269I GAATGC 2 cut(s) 486, 603
MvaI CCWGG 2 cut(s) 258, 508
MvnI CGCG 1 cut(s) 93
MwoI GCNNNNNNNGC 4 cut(s) 176, 216, 725, 1033
NciI CCSGG 1 cut(s) 377
NdeI CATATG 1 cut(s) 440
NdeII GATC 1 cut(s) 851
NlaIII CATG 3 cut(s) 476, 891, 922
NlaIV GGNNCC 2 cut(s) 220, 322
NmeAIII GCCGAG 1 cut(s) 205
NmuCI GTSAC 2 cut(s) 232, 610
NsiI ATGCAT 1 cut(s) 605
NspI RCATGY 1 cut(s) 922
PceI AGGCCT 1 cut(s) 210
PctI GAATGC 2 cut(s) 486, 603
PfeI GAWTC 4 cut(s) 149, 301, 737, 971
Pfl23II CGTACG 1 cut(s) 135
PinAI ACCGGT 1 cut(s) 25
PkrI GCNGC 5 cut(s) 533, 817, 964, 1026, 1038
Psp124BI GAGCTC 1 cut(s) 883
Psp6I CCWGG 2 cut(s) 256, 506
PspGI CCWGG 2 cut(s) 256, 506
PspLI CGTACG 1 cut(s) 135
PspN4I GGNNCC 2 cut(s) 220, 322
PspPI GGNCC 3 cut(s) 218, 254, 406
PstI CTGCAG 1 cut(s) 820
RsaI GTAC 2 cut(s) 137, 580
RsaNI GTAC 2 cut(s) 136, 579
RseI CAYNNNNRTG 1 cut(s) 930
SacI GAGCTC 1 cut(s) 883
SaqAI TTAA 2 cut(s) 896, 978
SatI GCNGC 5 cut(s) 532, 816, 963, 1025, 1037
Sau3AI GATC 1 cut(s) 851
Sau96I GGNCC 3 cut(s) 218, 254, 406
ScrFI CCNGG 3 cut(s) 258, 377, 508
SduI GDGCHC 1 cut(s) 883
SfaNI GCATC 3 cut(s) 266, 518, 590
SfcI CTRYAG 1 cut(s) 816
SfiI GGCCNNNNNGGCC 2 cut(s) 176, 216
SinI GGWCC 1 cut(s) 254
SmiMI CAYNNNNRTG 1 cut(s) 930
SmlI CTYRAG 1 cut(s) 449
SmoI CTYRAG 1 cut(s) 449
Sse9I AATT 7 cut(s) 64, 81, 366, 502, 615, 691, 935
SseBI AGGCCT 1 cut(s) 210
SsiI CCGC 2 cut(s) 76, 1025
SspI AATATT 1 cut(s) 800
SspMI CTAG 1 cut(s) 597
SstI GAGCTC 1 cut(s) 883
StuI AGGCCT 1 cut(s) 210
StyD4I CCNGG 3 cut(s) 256, 375, 506
TaaI ACNGT 1 cut(s) 135
TaiI ACGT 2 cut(s) 37, 147
TaqII GACCGA 1 cut(s) 540
TasI AATT 7 cut(s) 64, 81, 366, 502, 615, 691, 935
TatI WGTACW 1 cut(s) 578
TauI GCSGC 1 cut(s) 1027
TfiI GAWTC 4 cut(s) 149, 301, 737, 971
Tru1I TTAA 2 cut(s) 896, 978
Tru9I TTAA 2 cut(s) 896, 978
TscAI CASTG 1 cut(s) 913
TseFI GTSAC 2 cut(s) 232, 610
TseI GCWGC 4 cut(s) 531, 815, 962, 1036
Tsp45I GTSAC 2 cut(s) 232, 610
TspDTI ATGAA 7 cut(s) 17, 317, 342, 608, 668, 783, 835
TspGWI ACGGA 3 cut(s) 119, 131, 220
TspRI CASTG 1 cut(s) 913
VpaK11BI GGWCC 1 cut(s) 254
XapI RAATTY 4 cut(s) 64, 81, 502, 935
XbaI TCTAGA 1 cut(s) 596
XceI RCATGY 1 cut(s) 922
XspI CTAG 1 cut(s) 597
Zsp2I ATGCAT 1 cut(s) 605
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.