MD07G1188200.v1.1

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0008283
Physical Location & Seq
Forward (+)
26866877 .. 26870908
4032 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1188200.v1.1.491

Sequence Viewer

Length: 996 bp
ATGGCTTCGCTACCGTACGCCGAAGTGGATTTTAGCCTGAGATCAATGGCCGGTCGGGCCGAGGGCTTTGGCCGGTTCGCCATCGGAGGCCTCAACGGGCCGGTCTATTTCGTCACCACGTTGGCAGATGATGGTCCAGGCTCACTTCGTGAAGGATGCCGAAAACAAGAACCACTCTGGATTGTCTTTGAGGTTTCTGGCACCATTCACCTTTCATCTTACTTGAGCGTGTCGTCTTATAAGACAATTGATGGACGAGGTCAGAGGATAAAGCTCACGGGAAAGGGACTTAGGCTCAAGGAATGTGAGCATATAATTGTATGCAACCTCGAGTTTGAAGGTGGTAGAGGACATGATGTTGATGGCATTCAGATTAAACCGAACTCTAGGCACATATGGATAGACCGATGCAGCCTTCGTGATTATGATGATGGACTCATAGATATCACCAGACAAAGTACAGACATAACTATCTCTAGATGTTGCTTTGCACAACATGACAAGACGATGCTTATTGGAGCAGACCCCTCGCACGTTGGTGACAGATGCATTCGGGTGACAATCCATCATTGTTTTTTTGATGGAACAAGGCAAAGACAACCTCGTCTTAGATTTGGGAAAGTTCATCTTTACAACAATTACACTCGAAACTGGGGTATATATGCCGTTTGCGCCAGCATAGAATCCCAGATATACTCTCAATGCAACATTTATGAAGCAGGGAATAAGAAAAAAACCTTTGAATATTATACAGAAAAGGCAGCAGACAGAGAAGTGGCAAAGTCTGGCTTCATAATATCCGAGGGCGATCTATTCCTCAATGGAGCTCAACCATGCACATTGACAGGGTTCAGTGAAGAATGCCTGTTCCATCCAAGTGAATTTTATCCACAGTGGACTATGGAAGCAGCCTCAGATTCTCTTAAAGCCATTCTCCAAATTCTTACTGGCTGGCAATCCATTCATCGGCCAGCAGAGCAGGTGGTAACTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

332

Amino Acids

37.26

Weight (kDa)

6.6

Isoelectric Point (pI)

41.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectate_lyase_4 PF00544 75 - 246 1e-27 Pectate lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 240
AarI CACCTGC 1 cut(s) 970
AasI GACNNNNNNGTC 1 cut(s) 603
Acc36I ACCTGC 1 cut(s) 970
AccB1I GGYRCC 1 cut(s) 200
AcoI YGGCCR 3 cut(s) 48, 70, 968
AcsI RAATTY 2 cut(s) 881, 939
AdeI CACNNNGTG 1 cut(s) 149
AfaI GTAC 2 cut(s) 17, 460
AfiI CCNNNNNNNGG 1 cut(s) 966
AgsI TTSAA 2 cut(s) 338, 743
AjnI CCWGG 1 cut(s) 136
AleI CACNNNNGTG 1 cut(s) 537
AluBI AGCT 2 cut(s) 274, 827
AluI AGCT 2 cut(s) 274, 827
Alw21I GWGCWC 1 cut(s) 829
Ama87I CYCGRG 1 cut(s) 329
AoxI GGCC 6 cut(s) 48, 57, 70, 88, 98, 968
ApeKI GCWGC 3 cut(s) 411, 761, 908
ApoI RAATTY 2 cut(s) 881, 939
AspLEI GCGC 1 cut(s) 674
AspS9I GGNCC 3 cut(s) 57, 98, 134
AsuHPI GGTGA 5 cut(s) 106, 200, 439, 551, 568
AvaI CYCGRG 1 cut(s) 329
AvaII GGWCC 1 cut(s) 134
BanI GGYRCC 1 cut(s) 200
BanII GRGCYC 1 cut(s) 829
Bbv12I GWGCWC 1 cut(s) 829
BbvI GCAGC 3 cut(s) 423, 773, 920
BccI CCATC 8 cut(s) 89, 125, 245, 356, 425, 573, 575, 879
BceAI ACGGC 1 cut(s) 650
BcgI CGANNNNNNTGC 2 cut(s) 510, 544
BciT130I CCWGG 1 cut(s) 138
BfaI CTAG 2 cut(s) 387, 477
BfuAI ACCTGC 1 cut(s) 970
BglI GCCNNNNNGGC 1 cut(s) 56
BisI GCNGC 3 cut(s) 412, 762, 909
BlsI GCNGC 3 cut(s) 413, 763, 910
Bme1390I CCNGG 1 cut(s) 138
Bme18I GGWCC 1 cut(s) 134
BmeT110I CYCGRG 1 cut(s) 329
BmgT120I GGNCC 3 cut(s) 57, 98, 134
BmiI GGNNCC 1 cut(s) 202
BmrFI CCNGG 1 cut(s) 138
BmrI ACTGGG 1 cut(s) 661
BmsI GCATC 4 cut(s) 146, 398, 498, 536
BmuI ACTGGG 1 cut(s) 661
BpuEI CTTGAG 2 cut(s) 244, 281
BsaJI CCNNGG 2 cut(s) 60, 801
Bsc4I CCNNNNNNNGG 1 cut(s) 966
Bse118I RCCGGY 3 cut(s) 50, 72, 100
Bse1I ACTGG 2 cut(s) 656, 952
BseBI CCWGG 1 cut(s) 138
BseDI CCNNGG 2 cut(s) 60, 801
BseGI GGATG 2 cut(s) 161, 871
BseLI CCNNNNNNNGG 1 cut(s) 966
BseMII CTCAG 2 cut(s) 29, 927
BseNI ACTGG 2 cut(s) 656, 952
BseXI GCAGC 3 cut(s) 423, 773, 920
Bsh1285I CGRYCG 1 cut(s) 55
BshFI GGCC 6 cut(s) 50, 59, 72, 90, 100, 970
BshNI GGYRCC 1 cut(s) 200
BsiEI CGRYCG 1 cut(s) 55
BsiHKAI GWGCWC 1 cut(s) 829
BsiHKCI CYCGRG 1 cut(s) 329
BsiSI CCGG 3 cut(s) 51, 73, 101
BsiWI CGTACG 1 cut(s) 15
BslFI GGGAC 1 cut(s) 300
BslI CCNNNNNNNGG 1 cut(s) 966
BsmFI GGGAC 1 cut(s) 300
BsmI GAATGC 3 cut(s) 366, 549, 866
BsnI GGCC 6 cut(s) 50, 59, 72, 90, 100, 970
BsoBI CYCGRG 1 cut(s) 329
Bsp1286I GDGCHC 1 cut(s) 829
Bsp143I GATC 2 cut(s) 41, 808
BspANI GGCC 6 cut(s) 50, 59, 72, 90, 100, 970
BspCNI CTCAG 2 cut(s) 30, 926
BspLI GGNNCC 1 cut(s) 202
BspMI ACCTGC 1 cut(s) 970
BspT107I GGYRCC 1 cut(s) 200
BsrFI RCCGGY 3 cut(s) 50, 72, 100
BsrI ACTGG 2 cut(s) 656, 952
BssAI RCCGGY 3 cut(s) 50, 72, 100
BssECI CCNNGG 2 cut(s) 60, 801
BssMI GATC 2 cut(s) 41, 808
Bst2UI CCWGG 1 cut(s) 138
Bst4CI ACNGT 3 cut(s) 15, 894, 991
BstC8I GCNNGC 3 cut(s) 676, 953, 972
BstDEI CTNAG 4 cut(s) 38, 290, 608, 913
BstF5I GGATG 2 cut(s) 161, 871
BstHHI GCGC 1 cut(s) 674
BstKTI GATC 2 cut(s) 44, 811
BstMBI GATC 2 cut(s) 41, 808
BstMCI CGRYCG 1 cut(s) 55
BstMWI GCNNNNNNNGC 3 cut(s) 56, 671, 976
BstNI CCWGG 1 cut(s) 138
BstSCI CCNGG 1 cut(s) 136
BstV1I GCAGC 3 cut(s) 423, 773, 920
BsuRI GGCC 6 cut(s) 50, 59, 72, 90, 100, 970
BtsCI GGATG 2 cut(s) 161, 871
BtsIMutI CAGTG 2 cut(s) 859, 899
BveI ACCTGC 1 cut(s) 970
Cac8I GCNNGC 3 cut(s) 676, 953, 972
CfoI GCGC 1 cut(s) 674
Cfr10I RCCGGY 3 cut(s) 50, 72, 100
Cfr13I GGNCC 3 cut(s) 57, 98, 134
Csp6I GTAC 2 cut(s) 16, 459
CviAII CATG 3 cut(s) 353, 497, 834
CviQI GTAC 2 cut(s) 16, 459
DdeI CTNAG 4 cut(s) 38, 290, 608, 913
DpnI GATC 2 cut(s) 43, 810
DpnII GATC 2 cut(s) 41, 808
DraIII CACNNNGTG 1 cut(s) 149
DrdI GACNNNNNNGTC 1 cut(s) 603
DseDI GACNNNNNNGTC 1 cut(s) 603
EaeI YGGCCR 3 cut(s) 48, 70, 968
Ecl136II GAGCTC 1 cut(s) 827
Eco147I AGGCCT 1 cut(s) 90
Eco24I GRGCYC 1 cut(s) 829
Eco32I GATATC 1 cut(s) 445
Eco47I GGWCC 1 cut(s) 134
Eco53kI GAGCTC 1 cut(s) 827
Eco88I CYCGRG 1 cut(s) 329
EcoICRI GAGCTC 1 cut(s) 827
EcoRII CCWGG 1 cut(s) 136
EcoRV GATATC 1 cut(s) 445
EcoT22I ATGCAT 1 cut(s) 551
EcoT38I GRGCYC 1 cut(s) 829
FaeI CATG 3 cut(s) 356, 500, 837
FalI AAGNNNNNCTT 4 cut(s) 612, 644, 773, 805
FaqI GGGAC 1 cut(s) 300
FatI CATG 3 cut(s) 352, 496, 833
FauNDI CATATG 1 cut(s) 395
Fnu4HI GCNGC 3 cut(s) 412, 762, 909
FokI GGATG 2 cut(s) 168, 858
FriOI GRGCYC 1 cut(s) 829
Fsp4HI GCNGC 3 cut(s) 412, 762, 909
FspBI CTAG 2 cut(s) 387, 477
GlaI GCGC 1 cut(s) 673
GluI GCNGC 3 cut(s) 412, 762, 909
HaeIII GGCC 6 cut(s) 50, 59, 72, 90, 100, 970
HapII CCGG 3 cut(s) 51, 73, 101
HhaI GCGC 1 cut(s) 674
Hin1II CATG 3 cut(s) 356, 500, 837
Hin6I GCGC 1 cut(s) 672
HinP1I GCGC 1 cut(s) 672
HinfI GANTC 3 cut(s) 435, 683, 917
HpaII CCGG 3 cut(s) 51, 73, 101
HphI GGTGA 5 cut(s) 106, 200, 439, 551, 568
Hpy166II GTNNAC 1 cut(s) 897
Hpy188I TCNGA 5 cut(s) 86, 264, 372, 802, 916
Hpy188III TCNNGA 4 cut(s) 149, 178, 419, 477
Hpy8I GTNNAC 1 cut(s) 897
HpyAV CCTTC 3 cut(s) 146, 332, 425
HpyCH4III ACNGT 3 cut(s) 15, 894, 991
HpyCH4IV ACGT 2 cut(s) 119, 534
HpyCH4V TGCA 6 cut(s) 324, 411, 491, 549, 705, 837
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 671, 976
HpyF3I CTNAG 4 cut(s) 38, 290, 608, 913
HpySE526I ACGT 2 cut(s) 119, 534
Hsp92II CATG 3 cut(s) 356, 500, 837
HspAI GCGC 1 cut(s) 672
Kzo9I GATC 2 cut(s) 41, 808
LmnI GCTCC 2 cut(s) 518, 824
Lsp1109I GCAGC 3 cut(s) 423, 773, 920
LweI GCATC 4 cut(s) 146, 398, 498, 536
MaeI CTAG 2 cut(s) 387, 477
MaeII ACGT 2 cut(s) 119, 534
MaeIII GTNAC 4 cut(s) 112, 539, 556, 985
MalI GATC 2 cut(s) 43, 810
MboI GATC 2 cut(s) 41, 808
MboII GAAGA 1 cut(s) 869
MfeI CAATTG 1 cut(s) 246
MhlI GDGCHC 1 cut(s) 829
MluCI AATT 5 cut(s) 246, 315, 637, 881, 939
MlyI GAGTC 1 cut(s) 429
Mph1103I ATGCAT 1 cut(s) 551
MseI TTAA 2 cut(s) 375, 924
MslI CAYNNNNRTG 3 cut(s) 537, 554, 876
MspI CCGG 3 cut(s) 51, 73, 101
MspR9I CCNGG 1 cut(s) 138
MunI CAATTG 1 cut(s) 246
Mva1269I GAATGC 3 cut(s) 366, 549, 866
MvaI CCWGG 1 cut(s) 138
MwoI GCNNNNNNNGC 3 cut(s) 56, 671, 976
NdeI CATATG 1 cut(s) 395
NdeII GATC 2 cut(s) 41, 808
NlaIII CATG 3 cut(s) 356, 500, 837
NlaIV GGNNCC 1 cut(s) 202
NmeAIII GCCGAG 1 cut(s) 85
NmuCI GTSAC 3 cut(s) 112, 539, 556
NsiI ATGCAT 1 cut(s) 551
OliI CACNNNNGTG 1 cut(s) 537
PaeR7I CTCGAG 1 cut(s) 329
PaqCI CACCTGC 1 cut(s) 970
PceI AGGCCT 1 cut(s) 90
PctI GAATGC 3 cut(s) 366, 549, 866
PfeI GAWTC 2 cut(s) 683, 917
Pfl23II CGTACG 1 cut(s) 15
PflFI GACNNNGTC 1 cut(s) 258
PkrI GCNGC 3 cut(s) 413, 763, 910
PleI GAGTC 1 cut(s) 429
PpsI GAGTC 1 cut(s) 429
PsiI TTATAA 1 cut(s) 240
Psp124BI GAGCTC 1 cut(s) 829
Psp6I CCWGG 1 cut(s) 136
PspGI CCWGG 1 cut(s) 136
PspLI CGTACG 1 cut(s) 15
PspN4I GGNNCC 1 cut(s) 202
PspPI GGNCC 3 cut(s) 57, 98, 134
PspXI VCTCGAGB 1 cut(s) 329
PsyI GACNNNGTC 1 cut(s) 258
RsaI GTAC 2 cut(s) 17, 460
RsaNI GTAC 2 cut(s) 16, 459
RseI CAYNNNNRTG 3 cut(s) 537, 554, 876
SacI GAGCTC 1 cut(s) 829
SaqAI TTAA 2 cut(s) 375, 924
SatI GCNGC 3 cut(s) 412, 762, 909
Sau3AI GATC 2 cut(s) 41, 808
Sau96I GGNCC 3 cut(s) 57, 98, 134
SchI GAGTC 1 cut(s) 429
ScrFI CCNGG 1 cut(s) 138
SduI GDGCHC 1 cut(s) 829
SfaNI GCATC 4 cut(s) 146, 398, 498, 536
SfiI GGCCNNNNNGGCC 1 cut(s) 56
Sfr274I CTCGAG 1 cut(s) 329
SinI GGWCC 1 cut(s) 134
SlaI CTCGAG 1 cut(s) 329
SmiMI CAYNNNNRTG 3 cut(s) 537, 554, 876
SmlI CTYRAG 3 cut(s) 223, 296, 329
SmoI CTYRAG 3 cut(s) 223, 296, 329
Sse9I AATT 5 cut(s) 246, 315, 637, 881, 939
SseBI AGGCCT 1 cut(s) 90
SspI AATATT 1 cut(s) 746
SspMI CTAG 2 cut(s) 387, 477
SstI GAGCTC 1 cut(s) 829
StuI AGGCCT 1 cut(s) 90
StyD4I CCNGG 1 cut(s) 136
TaaI ACNGT 3 cut(s) 15, 894, 991
TaiI ACGT 2 cut(s) 122, 537
TaqI TCGA 2 cut(s) 330, 646
TaqII GACCGA 1 cut(s) 420
TasI AATT 5 cut(s) 246, 315, 637, 881, 939
TatI WGTACW 1 cut(s) 458
TfiI GAWTC 2 cut(s) 683, 917
Tru1I TTAA 2 cut(s) 375, 924
Tru9I TTAA 2 cut(s) 375, 924
TscAI CASTG 2 cut(s) 859, 899
TseFI GTSAC 3 cut(s) 112, 539, 556
TseI GCWGC 3 cut(s) 411, 761, 908
Tsp45I GTSAC 3 cut(s) 112, 539, 556
TspDTI ATGAA 5 cut(s) 204, 614, 729, 781, 953
TspRI CASTG 2 cut(s) 859, 899
Tth111I GACNNNGTC 1 cut(s) 258
VpaK11BI GGWCC 1 cut(s) 134
XapI RAATTY 2 cut(s) 881, 939
XbaI TCTAGA 1 cut(s) 476
XcmI CCANNNNNNNNNTGG 1 cut(s) 944
XhoI CTCGAG 1 cut(s) 329
XspI CTAG 2 cut(s) 387, 477
Zsp2I ATGCAT 1 cut(s) 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.