Rmu_sc0004695.1_g000004

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004695.1
OG0008283
Physical Location & Seq
Forward (+)
14081 .. 15258
1178 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004695.1_g000004.1.cds

Sequence Viewer

Length: 888 bp
atgttttggtttttagatgatggtccaggctcacttcgtgaaggatgccggaaacaagaaccactttggattgtctttgaggtttccggcaccattattctctcttcttacttaagtgtgtcatcttacaagacaattgatggccgagggcagagagtaaagctcacaggcaagggccttagactgaaggaatgtgaacacataatcatatgcaaccttgagtttgaaggtggtagaggacatgatgttgatggcattcagataaaaccaaattccaggcatatatggatcgaccgatgcagccttcgtgattatgatgatggactcatagatatcacccgacaaagtacagacataactatttctagatgttacttccatcaacatgacaagacgatgcttattggagcggacccttcacatgttggtgacagatgcattcgggtcactattcatcattgtttttttgatgggacaaggcaaaggcaacctcgtgttagatttgggaaagttcatctttacaacaattacactcgtaactggggtatatatgctgtttgtgccagcgtagaatcccagatatactctcaatgcaacatatatgaagcaggaacgaagaagaagacgtttgaatattatacagaaaaggctgcagacagagaagtggcgaaatctggcctcataagatctgagggggacgtattcctgaatggagcccaaccatgcacattaacagggttcagtgaagaatgcatgttccatccaagtgaattttatccactttggactatggaagcagcctcagattctcttaaaggtgttctccacattcttacgggttggcaatccattcataggccaccagtagagcagctggtaatggtatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

295

Amino Acids

33.77

Weight (kDa)

6.85

Isoelectric Point (pI)

42.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 89
AccBSI CCGCTC 1 cut(s) 410
AciI CCGC 1 cut(s) 410
AclWI GGATC 1 cut(s) 296
AcoI YGGCCR 1 cut(s) 142
AcsI RAATTY 2 cut(s) 271, 770
AcuI CTGAAG 1 cut(s) 206
AdeI CACNNNGTG 1 cut(s) 38
AfaI GTAC 1 cut(s) 349
AfiI CCNNNNNNNGG 1 cut(s) 855
AflII CTTAAG 1 cut(s) 112
AflIII ACRYGT 1 cut(s) 421
AgsI TTSAA 2 cut(s) 227, 632
AjnI CCWGG 2 cut(s) 25, 275
AluBI AGCT 2 cut(s) 163, 874
AluI AGCT 2 cut(s) 163, 874
AlwI GGATC 1 cut(s) 296
AoxI GGCC 4 cut(s) 142, 175, 676, 857
ApeKI GCWGC 4 cut(s) 300, 650, 797, 871
ApoI RAATTY 2 cut(s) 271, 770
AspS9I GGNCC 3 cut(s) 23, 175, 412
AsuHPI GGTGA 2 cut(s) 328, 440
AvaII GGWCC 2 cut(s) 23, 412
BanI GGYRCC 1 cut(s) 89
BanII GRGCYC 1 cut(s) 718
BauI CACGAG 1 cut(s) 492
BbsI GAAGAC 1 cut(s) 629
BbvI GCAGC 4 cut(s) 312, 637, 809, 883
BccI CCATC 7 cut(s) 14, 134, 245, 314, 387, 464, 768
BciT130I CCWGG 2 cut(s) 27, 277
BfaI CTAG 1 cut(s) 366
BfmI CTRYAG 1 cut(s) 651
BfrI CTTAAG 1 cut(s) 112
BglII AGATCT 1 cut(s) 686
BisI GCNGC 4 cut(s) 301, 651, 798, 872
BlsI GCNGC 4 cut(s) 302, 652, 799, 873
Bme1390I CCNGG 2 cut(s) 27, 277
Bme18I GGWCC 2 cut(s) 23, 412
BmgT120I GGNCC 3 cut(s) 23, 175, 412
BmiI GGNNCC 3 cut(s) 91, 414, 715
BmrFI CCNGG 2 cut(s) 27, 277
BmrI ACTGGG 1 cut(s) 550
BmsI GCATC 4 cut(s) 35, 287, 387, 425
BmuI ACTGGG 1 cut(s) 550
BpiI GAAGAC 1 cut(s) 629
BplI GAGNNNNNCTC 2 cut(s) 147, 179
BpuEI CTTGAG 1 cut(s) 239
BsaJI CCNNGG 1 cut(s) 145
Bsc4I CCNNNNNNNGG 1 cut(s) 855
Bse1I ACTGG 2 cut(s) 545, 863
BseBI CCWGG 2 cut(s) 27, 277
BseDI CCNNGG 1 cut(s) 145
BseGI GGATG 2 cut(s) 50, 760
BseLI CCNNNNNNNGG 1 cut(s) 855
BseMII CTCAG 2 cut(s) 681, 816
BseNI ACTGG 2 cut(s) 545, 863
BseXI GCAGC 4 cut(s) 312, 637, 809, 883
Bsh1285I CGRYCG 1 cut(s) 295
BshFI GGCC 4 cut(s) 144, 177, 678, 859
BshNI GGYRCC 1 cut(s) 89
BsiEI CGRYCG 1 cut(s) 295
BsiSI CCGG 2 cut(s) 49, 87
BslFI GGGAC 2 cut(s) 487, 710
BslI CCNNNNNNNGG 1 cut(s) 855
BsmFI GGGAC 2 cut(s) 487, 710
BsmI GAATGC 3 cut(s) 255, 438, 755
BsnI GGCC 4 cut(s) 144, 177, 678, 859
Bsp1286I GDGCHC 1 cut(s) 718
Bsp143I GATC 2 cut(s) 288, 686
BspACI CCGC 1 cut(s) 410
BspANI GGCC 4 cut(s) 144, 177, 678, 859
BspCNI CTCAG 2 cut(s) 682, 815
BspLI GGNNCC 3 cut(s) 91, 414, 715
BspMAI CTGCAG 1 cut(s) 655
BspPI GGATC 1 cut(s) 296
BspT107I GGYRCC 1 cut(s) 89
BspTI CTTAAG 1 cut(s) 112
BsrBI CCGCTC 1 cut(s) 410
BsrI ACTGG 2 cut(s) 545, 863
BssECI CCNNGG 1 cut(s) 145
BssMI GATC 2 cut(s) 288, 686
BssSI CACGAG 1 cut(s) 492
Bst2BI CACGAG 1 cut(s) 492
Bst2UI CCWGG 2 cut(s) 27, 277
Bst6I CTCTTC 1 cut(s) 109
BstAFI CTTAAG 1 cut(s) 112
BstC8I GCNNGC 1 cut(s) 565
BstDEI CTNAG 3 cut(s) 179, 690, 802
BstF5I GGATG 2 cut(s) 50, 760
BstKTI GATC 2 cut(s) 291, 689
BstMBI GATC 2 cut(s) 288, 686
BstMCI CGRYCG 1 cut(s) 295
BstMWI GCNNNNNNNGC 1 cut(s) 560
BstNI CCWGG 2 cut(s) 27, 277
BstNSI RCATGY 2 cut(s) 425, 757
BstSCI CCNGG 2 cut(s) 25, 275
BstSFI CTRYAG 1 cut(s) 651
BstV1I GCAGC 4 cut(s) 312, 637, 809, 883
BstV2I GAAGAC 1 cut(s) 629
BstX2I RGATCY 1 cut(s) 686
BstYI RGATCY 1 cut(s) 686
BsuRI GGCC 4 cut(s) 144, 177, 678, 859
BtsCI GGATG 2 cut(s) 50, 760
BtsIMutI CAGTG 1 cut(s) 748
Cac8I GCNNGC 1 cut(s) 565
Cfr13I GGNCC 3 cut(s) 23, 175, 412
Csp6I GTAC 1 cut(s) 348
CviAII CATG 5 cut(s) 242, 386, 422, 723, 754
CviQI GTAC 1 cut(s) 348
DdeI CTNAG 3 cut(s) 179, 690, 802
DpnI GATC 2 cut(s) 290, 688
DpnII GATC 2 cut(s) 288, 686
DraIII CACNNNGTG 1 cut(s) 38
EaeI YGGCCR 1 cut(s) 142
Eam1104I CTCTTC 1 cut(s) 109
EarI CTCTTC 1 cut(s) 109
Eco24I GRGCYC 1 cut(s) 718
Eco32I GATATC 1 cut(s) 334
Eco47I GGWCC 2 cut(s) 23, 412
Eco57I CTGAAG 1 cut(s) 206
EcoO109I RGGNCCY 1 cut(s) 175
EcoRII CCWGG 2 cut(s) 25, 275
EcoRV GATATC 1 cut(s) 334
EcoT22I ATGCAT 2 cut(s) 440, 755
EcoT38I GRGCYC 1 cut(s) 718
FaeI CATG 5 cut(s) 245, 389, 425, 726, 757
FalI AAGNNNNNCTT 4 cut(s) 48, 80, 501, 533
FaqI GGGAC 2 cut(s) 487, 710
FatI CATG 5 cut(s) 241, 385, 421, 722, 753
FauNDI CATATG 1 cut(s) 209
Fnu4HI GCNGC 4 cut(s) 301, 651, 798, 872
FokI GGATG 2 cut(s) 57, 747
FriOI GRGCYC 1 cut(s) 718
Fsp4HI GCNGC 4 cut(s) 301, 651, 798, 872
FspBI CTAG 1 cut(s) 366
GluI GCNGC 4 cut(s) 301, 651, 798, 872
HaeIII GGCC 4 cut(s) 144, 177, 678, 859
HapII CCGG 2 cut(s) 49, 87
Hin1II CATG 5 cut(s) 245, 389, 425, 726, 757
HinfI GANTC 3 cut(s) 324, 572, 806
HpaII CCGG 2 cut(s) 49, 87
HphI GGTGA 2 cut(s) 328, 440
Hpy166II GTNNAC 1 cut(s) 197
Hpy188I TCNGA 3 cut(s) 261, 691, 805
Hpy188III TCNNGA 4 cut(s) 38, 308, 366, 706
Hpy8I GTNNAC 1 cut(s) 197
HpyAV CCTTC 5 cut(s) 35, 181, 221, 314, 426
HpyCH4IV ACGT 2 cut(s) 626, 699
HpyCH4V TGCA 7 cut(s) 213, 300, 438, 594, 653, 726, 753
HpyF10VI GCNNNNNNNGC 1 cut(s) 560
HpyF3I CTNAG 3 cut(s) 179, 690, 802
HpySE526I ACGT 2 cut(s) 626, 699
Hsp92II CATG 5 cut(s) 245, 389, 425, 726, 757
Kzo9I GATC 2 cut(s) 288, 686
LmnI GCTCC 2 cut(s) 407, 713
Lsp1109I GCAGC 4 cut(s) 312, 637, 809, 883
LweI GCATC 4 cut(s) 35, 287, 387, 425
MaeI CTAG 1 cut(s) 366
MaeII ACGT 2 cut(s) 626, 699
MaeIII GTNAC 4 cut(s) 371, 428, 445, 536
MalI GATC 2 cut(s) 290, 688
MbiI CCGCTC 1 cut(s) 410
MboI GATC 2 cut(s) 288, 686
MboII GAAGA 5 cut(s) 96, 628, 631, 634, 758
MfeI CAATTG 1 cut(s) 135
MflI RGATCY 1 cut(s) 686
MhlI GDGCHC 1 cut(s) 718
MluCI AATT 4 cut(s) 135, 271, 526, 770
MlyI GAGTC 1 cut(s) 318
MnlI CCTC 7 cut(s) 73, 140, 230, 501, 685, 689, 811
Mph1103I ATGCAT 2 cut(s) 440, 755
MseI TTAA 3 cut(s) 113, 731, 813
MslI CAYNNNNRTG 3 cut(s) 384, 426, 765
MspA1I CMGCKG 1 cut(s) 874
MspCI CTTAAG 1 cut(s) 112
MspI CCGG 2 cut(s) 49, 87
MspR9I CCNGG 2 cut(s) 27, 277
MunI CAATTG 1 cut(s) 135
Mva1269I GAATGC 3 cut(s) 255, 438, 755
MvaI CCWGG 2 cut(s) 27, 277
MwoI GCNNNNNNNGC 1 cut(s) 560
NdeI CATATG 1 cut(s) 209
NdeII GATC 2 cut(s) 288, 686
NlaIII CATG 5 cut(s) 245, 389, 425, 726, 757
NlaIV GGNNCC 3 cut(s) 91, 414, 715
NmeAIII GCCGAG 1 cut(s) 170
NmuCI GTSAC 2 cut(s) 428, 445
NsiI ATGCAT 2 cut(s) 440, 755
NspI RCATGY 2 cut(s) 425, 757
PciI ACATGT 1 cut(s) 421
PctI GAATGC 3 cut(s) 255, 438, 755
PfeI GAWTC 2 cut(s) 572, 806
PkrI GCNGC 4 cut(s) 302, 652, 799, 873
PleI GAGTC 1 cut(s) 318
PpsI GAGTC 1 cut(s) 318
PscI ACATGT 1 cut(s) 421
Psp6I CCWGG 2 cut(s) 25, 275
PspGI CCWGG 2 cut(s) 25, 275
PspN4I GGNNCC 3 cut(s) 91, 414, 715
PspPI GGNCC 3 cut(s) 23, 175, 412
PstI CTGCAG 1 cut(s) 655
PsuI RGATCY 1 cut(s) 686
PvuII CAGCTG 1 cut(s) 874
RsaI GTAC 1 cut(s) 349
RsaNI GTAC 1 cut(s) 348
RseI CAYNNNNRTG 3 cut(s) 384, 426, 765
SaqAI TTAA 3 cut(s) 113, 731, 813
SatI GCNGC 4 cut(s) 301, 651, 798, 872
Sau3AI GATC 2 cut(s) 288, 686
Sau96I GGNCC 3 cut(s) 23, 175, 412
SchI GAGTC 1 cut(s) 318
ScrFI CCNGG 2 cut(s) 27, 277
SduI GDGCHC 1 cut(s) 718
SetI ASST 9 cut(s) 84, 165, 219, 232, 493, 629, 702, 820, 876
SfaNI GCATC 4 cut(s) 35, 287, 387, 425
SfcI CTRYAG 1 cut(s) 651
SinI GGWCC 2 cut(s) 23, 412
SmiMI CAYNNNNRTG 3 cut(s) 384, 426, 765
SmlI CTYRAG 2 cut(s) 112, 218
SmoI CTYRAG 2 cut(s) 112, 218
Sse9I AATT 4 cut(s) 135, 271, 526, 770
SsiI CCGC 1 cut(s) 410
SspI AATATT 1 cut(s) 635
SspMI CTAG 1 cut(s) 366
StyD4I CCNGG 2 cut(s) 25, 275
TaiI ACGT 2 cut(s) 629, 702
TaqI TCGA 1 cut(s) 291
TaqII GACCGA 1 cut(s) 309
TasI AATT 4 cut(s) 135, 271, 526, 770
TatI WGTACW 1 cut(s) 347
TfiI GAWTC 2 cut(s) 572, 806
Tru1I TTAA 3 cut(s) 113, 731, 813
Tru9I TTAA 3 cut(s) 113, 731, 813
TscAI CASTG 1 cut(s) 748
TseFI GTSAC 2 cut(s) 428, 445
TseI GCWGC 4 cut(s) 300, 650, 797, 871
Tsp45I GTSAC 2 cut(s) 428, 445
TspDTI ATGAA 4 cut(s) 443, 503, 618, 842
TspRI CASTG 1 cut(s) 748
Vha464I CTTAAG 1 cut(s) 112
VpaK11BI GGWCC 2 cut(s) 23, 412
XapI RAATTY 2 cut(s) 271, 770
XbaI TCTAGA 1 cut(s) 365
XceI RCATGY 2 cut(s) 425, 757
XspI CTAG 1 cut(s) 366
Zsp2I ATGCAT 2 cut(s) 440, 755
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.